ライフサイエンスコーパス: expression vector

1 ner, as a result of the design of a novel co-expression vector.

2 bone marrow cells transfected with a Prdm14 expression vector.

3 possibility of using the virus as a vaccine expression vector.

4 ized and cloned into a small interfering RNA expression vector.

5 ulated by co-transfection with the C/EBPbeta expression vector.

6 in vivo using a transposon-based eukaryotic expression vector.

7 transfection of an exogenous PPARbeta/delta expression vector.

8 7 cells stably transfected with an aromatase expression vector.

9 ary DNA (cDNA) using pc DNA3.1 (+)/Myc-His A expression vector.

10 cells, which lack EGFR expression, with EGFR expression vector.

11 stem (ES) cells were transfected with a Tlx3 expression vector.

12 NA induction by a constitutively active CHK2 expression vector.

13 poprotein genes were cloned into a mammalian expression vector.

14 a cells were stably transfected with a DAbR1 expression vector.

15 ompatible and reading frame-compatible plant expression vector.

16 osiphum padi virus (RhPV) into a baculovirus expression vector.

17 and TIMP2 cDNA were cloned into a mammalian expression vector.

18 over cells transfected with the parent eGFP expression vector.

19 encoding Firefly luciferase in a dicistronic expression vector.

20 rexpressing PKC-delta using a Zn2+ inducible expression vector.

21 falciparum and cloned this into a mammalian expression vector.

22 erexpressed via transient transfection of an expression vector.

23 nally into a zero background BsaI/ccdB-based expression vector.

24 inal end to generate CPD-MAGE-A3 in a pQE-70 expression vector.

25 ovel hypoxia and hepatoma dual specific gene expression vector.

26 nalyses using a zucchini yellow mosaic virus expression vector.

27 d cell lines and cell lines transfected with expression vectors.

28 tituting them with mutant and wild-type TRIF expression vectors.

29 mal RNA and transcripts of their baculoviral expression vectors.

30 epG2 cells stably transduced with lentiviral expression vectors.

31 RV vaccines, better antiviral therapies, and expression vectors.

32 ZF-TF that can be transferred to a number of expression vectors.

33 FK506 and transfected with GRbeta and FKBP51 expression vectors.

34 and independent of cloning dsRNAs into plant expression vectors.

35 fied by PCR and cloned into Escherichia coli expression vectors.

36 ughput transfer of ORF inserts into suitable expression vectors.

37 e constructed using product similarity among expression vectors.

38 tion of either of two dominant-negative CREB expression vectors.

39 and SUMO-1 (small ubiquitin-like modifier-1) expression vectors.

40 ked promoter:BvLz lines with additional BvLz expression vectors.

41 ave practical implications for the design of expression vectors.

42 were transfected with EpoR and/or RNF41 gene expression vectors.

43 pid and efficient cloning of gRNA pairs into expression vectors.

44 rturbation libraries that contain dual-guide expression vectors.

45 nically or with adeno-associated virus (AAV) expression vectors.

46 lls that overexpressed GATA6 from lentiviral expression vectors.

47 the TF sequences into a number of different expression vectors.

48 cable means to limit off-targeting from RNAi expression vectors.

49 e relative concentrations of the Flp and Int expression vectors.

50 genes are typically transferred to mammalian expression vectors, a major rate-limiting step in the it

51 Transfection of HUVECs with MCPIP expression vector activated the expression of cdh12 and

52 e recombinases-the amount of the recombinase expression vectors added at transfection-and on the orde

53 pression assays (agroinfiltration) and virus expression vectors (agroinfection) to express functional

54 hain variable regions were subcloned into an expression vector allowing the production of recombinant

55 iently transfected with a wild-type (WT) p53 expression vector along with a luciferase reporter conta

56 with the LmGT4 gene on a multicopy episomal expression vector also reverted these phenotypes, confir

57 Using a Pichinde virus (PICV) GP expression vector and a PICV reverse genetics system, we

58 bly expressing a Tet-on RORalpha or RORgamma expression vector and a ROR-responsive element (RORE)-LU

59 cloning of the TM1418-TM1419 operon in pBAD expression vector and confirmed to function in the crude

60 s stably transfected with a HIF-1alpha siRNA expression vector and found that HIF-1alpha mRNA silenci

61 th the pLucRLuc:p53/Wrap53beta bidirectional expression vector and known p53 transcriptional regulato

62 The DNA encoding lpt3 was cloned into an expression vector and Lpt3 was purified.

63 taVIII was examined by subcloning it into an expression vector and raising an antibody specific to PK

64 ESRP1 expression vector and small interference RNA (siRNA) tar

65 ies in soluble form from an Escherichia coli expression vector and the enzyme stripped the PDGA capsu

66 cNr, the coding region was subcloned into an expression vector and transformed into Escherichia coli.

67 Notably, when agaF+ was cloned into an expression vector and transformed into six spinach isola

68 collection was transferred into the relevant expression vector and used to produce a protein microarr

69 ive and culm-regulated promoters on separate expression vectors and combinatorial plant transformatio

70 Compared to conventional plasmid-based expression vectors and donor templates, we show that com

71 he viral glycoproteins and CD163 receptor in expression vectors and examined their expression and int

72 g, easily cultivated haloarchaeon, including expression vectors and gene-deletion strategies, includi

73 ro and were able to transfect them with gene-expression vectors and incorporate them into the gonads

74 reproducible protocol for electroporation of expression vectors and morpholino oligonucleotides into

75 erapeutic agents, short interfering RNA, DNA expression vectors and proteins to different types of ce

76 irus replication and lead to improvements in expression vectors and recombinant vaccines.

77 hich the mutant libraries are transferred to expression vectors and screened for phenotypes of intere

78 and knockdown were performed using specific expression vectors and siRNA technology, respectively.

79 inatorial assembly of functional (multi)gene expression vectors and their efficient and specific targ

80 lified by RT-PCR and nested PCR, cloned into expression vectors and transfected into a human cell lin

81 olactin beta was inserted into two different expression vectors and transfected into E. coli or P. pa

82 gated for their applications as vaccines and expression vectors and, more recently, because of concer

83 trate, using an miR-10b synthetic precursor, expression vector, and antisense oligonucleotide, that m

84 ll-length cDNA was cloned into a baculovirus expression vector, and recombinant Aed a 3 (rAed a 3) wa

85 The MHC-I cDNA was cloned into a mammalian expression vector, and stable B78H1 cell lines were gene

86 althy T cells by transfection with CREMalpha expression vector, and Syk expression and phosphorylatio

87 were cloned individually into a prokaryotic expression vector, and their gene products were purified

88 atient-derived serum samples, cloned into an expression vector, and used to generate viral pseudopart

89 Once the proper expression vectors are cloned, our protocol should allow

90 Virus-based expression vectors are commonplace tools for the product

91 Baculovirus expression vectors are used for introducing the AAV gene

92 Multicloning sites (MCSs) in standard expression vectors are widely used and thought to be ben

93 nhibits expression of exogenous MDM2 from an expression vector as long as the vector contains an AU-/

94 These genes were cloned into three expression vectors as native sequences and as N-terminal

95 Optimization of codon use in expression vectors, as expected, restores efficient prot

96 Fragments are then combined with linearized expression vectors, assembled in vitro as part of a sequ

97 er, IL-21 in a synergistic manner with IL-15 expression vector augmented the vaccine-induced recall r

98 This method utilizing the antibody expression vectors, available at Addgene, has many appli

99 f fully assembled ZFNs into Escherichia coli expression vectors, bacterial lysates were found to be m

100 Here, a series of expression vectors based on Oryza sativa MIR390 (OsMIR39

101 ribes the construction of a set of versatile expression vectors based on the In-Fusion cloning enzyme

102 cialties, the design of application-specific expression vectors becomes the new norm.

103 tudying EBV capsid assembly, the baculovirus expression vector (BEV) system was used to express the c

104 e cell is infected with a single baculovirus expression vector (BEV).

105 adenoviral transduction of dominant negative expression vectors blocked IL-17-mediated CRP induction.

106 d vector that can be rapidly converted to an expression vector by a simple restriction enzyme digesti

107 Tobacco mosaic virus (TMV)-based transient expression vectors can express very high levels of forei

108 linical studies is developing a suitable CAR expression vector capable of genetically modifying a bro

109 Considerations of expression vector choice, modifications to sequence, tro

110 A mammalian expression vector coding for FLAG-tagged human ADAMTS4 w

111 We initially constructed a beta-globin expression vector composed of the hybrid cytomegalovirus

112 targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused

113 Transfection of CHO cells with a GPIHBP1 expression vector confers on cells the ability to bind b

114 Fusion expression vectors contained either bacterial alkaline p

115 cotransfected into TM cells with a mammalian expression vector containing a dominant-negative mutant

116 s, we stably transfected INS-1 cells with an expression vector containing the gene for the DNA repair

117 Using an expression vector containing the minimal tissue factor p

118 Co-transformation of an expression vector containing this gene and pSUPAR6-L3-3S

119 Expression vectors containing constitutively active CaMK

120 Two tissue specific fluorescent expression vectors containing either a GFP or mCherry tr

121 By delivering expression vectors containing either wild-type or mutant

122 ently transfected with combinations of human expression vectors containing genes for TLR2, TLR1, and

123 ategy described here to synthesize mammalian expression vectors containing site-specific cisplatin IC

124 binary vectors from pGWBs series to produce expression vectors containing the stevia's UGT, enables

125 Cotransfection of RNA and an I-SceI expression vector demonstrated insertion of RNA-derived

126 A new protein expression vector design utilizing an N-terminal six-his

127 In this work, a new GFP expression vector designated pUAS-Neostinger was constru

128 Protein expression vectors designed for large-scale protein prod

129 process, we constructed a series of plasmid expression vectors, each containing a different type II

130 novel transgenic mouse model with this EGFP expression vector (EGFP-mdx23 Tg).

131 Transfection of an expression vector encoding constitutively active IKK2 in

132 e inflammasome adaptor ASC, transfected with expression vectors encoding A1PI-M or A1PI-Z.

133 Immunization with plasmid expression vectors encoding hemagglutinin (HA) elicited

134 Expression vectors encoding mutants of the Ras/mitogen-a

135 Cotransfections with expression vectors encoding NF-kappaB subunits were carr

136 We constructed expression vectors encoding short fusion peptides corres

137 -transfections was performed using mammalian expression vectors encoding specific glycosyltransferase

138 a series of co-transfections using mammalian expression vectors encoding specific glycosyltransferase

139 transfection of a luciferase construct with expression vectors following MITF siRNA revealed that TF

140 yeast to design a single genomic capture and expression vector for antibiotic production in Bacillus

141 epatocytes were transduced with a lentiviral expression vector for Cd47 using a refined in vitro tran

142 zyme sites for inserting the product into an expression vector for cloning purposes.

143 Cloning of the gene in an expression vector for ex vivo cell-cell fusion and pseud

144 expression, PC12 cells were transfected with expression vector for full length or truncated inactive

145 hage display are typically subcloned into an expression vector for further biochemical characterizati

146 biotechnological applications, is used as an expression vector for gene delivery in mammalian cells.

147 Introduction of an expression vector for KLF15 short hairpin RNA, together

148 nd metastatic by stable transfection with an expression vector for OPN to identify RAN GTPase (RAN) a

149 ransfection of noninvasive R37 cells with an expression vector for RAN resulted in increased anchorag

150 id (MIP) is an alternative, robust transgene expression vector for reprogramming.

151 Transfection of an expression vector for S100P into a benign, nonmetastatic

152 HepG2 cells transfected with an expression vector for the active nuclear form of SREBP-1

153 a one-step cloning, into a binary multigene expression vector for transient or stable coexpression w

154 Finally, we modified existing expression vectors for 19 epitope-tagged human WNT prote

155 n of fluorescent CNS reporter constructs and expression vectors for constitutively active signaling p

156 We report the generation of versatile expression vectors for detection and isolation of protei

157 transport genes and Gateway((R))-compatible expression vectors for functional analysis and subcellul

158 Introduction of expression vectors for miR-203 into keratinocytes that s

159 Introduction of expression vectors for miR-424 reduced both the levels o

160 be easily adapted for developing specialized expression vectors for other organisms, zero background

161 ein we report development and testing of new expression vectors for Sindbis, Chikungunya, and eastern

162 ric mouse-human genes were cloned into plant expression vectors for stable nuclear transformation of

163 We built cDNA expression vectors for the 13 previously undescribed mis

164 ith a CYP3A4 proximal promoter construct and expression vectors for the NFI isoforms NFIA1.1, NFIB2,

165 Here, we report construction of new expression vectors for two Old World arthritogenic alpha

166 r RNA, and development of poxviruses as gene expression vectors for use as recombinant vaccines.

167 in mice in the absence of tumor cells using expression vectors for VEGF-A(164).

168 nzymes, HEK293-F cells were transfected with expression vectors for visual cycle proteins and co-tran

169 Expression vectors for wild-type and mutant WNT10A were

170 -/-) MEFs that were infected with adenoviral expression vectors for Zmiz2.

171 ibe a detailed protocol for constructing ZFN expression vectors, for generating and introducing ZFN-e

172 In contrast, genomic DNA expression vectors generate physiologically-relevant lev

173 Expression vectors generated by in vitro recombination o

174 oding hemagglutinin-tagged, full-length PUMA expression vector (HA-PUMA), PUMA lacking the Bcl-2 homo

175 lin G (IgG) 5H2, originally produced from an expression vector, has been shown to be a variant contai

176 MBP-facilitated crystallization, a series of expression vectors have been designed with either a shor

177 Improved expression vectors have been tested for protein expressi

178 strate the feasibility of miRNA targeting of expression vector helper functions that are provided in

179 uggest that rotaviruses will prove useful as expression vectors.IMPORTANCE Rotaviruses are a major ca

180 ters was significantly augmented by an LRH-1 expression vector in a co-transfection assay.

181 wo guide RNAs (gRNAs) and Cas9 from a single expression vector in combination with single-cell clonin

182 As to be assembled into a single baculoviral expression vector in only two steps.

183 hree overlapping fragments from a lentiviral expression vector in the epidermis of living skin equiva

184 lytic form of viral replication with a BZLF1 expression vector in the presence and absence of various

185 wo guide RNAs (gRNAs) and Cas9 from a single expression vector in transfected cells in combination wi

186 First, we built an expression vector in which the Oct3/4 promoter drives th

187 Using expression vectors in 293T cells or Jurkat T cells, we s

188 Coexpression of WT TLR4, CD14, and MD-2 expression vectors in HEK293T cells was first optimized

189 athogens that have been developed as protein expression vectors in insect cells and as transduction v

190 by transfection with C/EBPbeta and NF-kappaB expression vectors in the presence or absence of IL-1bet

191 Gene transfer using an adenoviral hRAMP1 expression vector increased the maximal production of cA

192 Transfection of HUVECs with an MCPIP expression vector induced angiogenesis-related genes and

193 stability, we found that transfection of ZFN-expression vectors induced up to a 15-fold increase in c

194 and prostate cancer cells with a DLC-3alpha expression vector inhibited cell proliferation, colony f

195 transfected with a doxycycline-regulated AHR expression vector, inhibition of AHR expression causes a

196 A Ski siRNA expression vector injected into nude mice resulted in a

197 Using YFP-fusion gene expression vectors, interaction between 14-3-3zeta and c

198 initransposon that can function as a protein expression vector into a plasmid that contains the open

199 of CRC cells stably transfected with a Cten expression vector into nude mice and (b) testing a serie

200 By electroporating a cre-recombinase expression vector into the cortex of E13.5 embryos carry

201 into the node, and electroporation of a GFP expression vector into the midline neural plate, reveale

202 cell lines by stable transfection of ERalpha expression vectors into ER- breast cancer BCap37 cells.

203 16-specific DNA methyltransferase (P16-dnmt) expression vector is designed using a P16 promoter-speci

204 The newly created expression vector is expected to enhance transgene expre

205 delivery systems and hypoxia-regulated gene expression vectors is the most important factors.

206 Introducing a LSD1 expression vector lacking the miR-137 recognition site r

207 reversed by re-expression of FUNDC1 and NIX expression vectors lacking the miR-137 recognition sites

208 nt into a heterologous intron in a mammalian expression vector markedly reduced the expression of the

209 Finally, we demonstrate that the ble-2A expression vector may be used to fluorescently label an

210 lower in KO mice than in cell lines via RGN expression vector-mediated genome editing.

211 were first implemented as heterologous gene expression vectors more than three decades ago.

212 Introducing an expression vector of Tlx or cyclin D1 that lacks the let

213 rix indicating the cosine similarity between expression vectors of genes in this database.

214 For hippocampal CA3-->CA1 synapses, AAV expression vectors of serotype 1, 5, and 8 led to light-

215 a/K-ATPase alpha1 knockdown PY-17 cells with expression vectors of wild type or mutant alpha1 carryin

216 Episomal gene expression vectors offer a safe and attractive alternati

217 -008a were expressed from the same mammalian expression vector, only Ser5-001 was stably expressed, w

218 rescued by ectopic expression of a PKCalpha expression vector or an active Src.

219 th mu-36p66ShcA (micro-36) dominant negative expression vector or isoform-specific p66-small interfer

220 e transfected cells with a wild-type GPIHBP1 expression vector or mutant GPIHBP1 vectors in which spe

221 gether, our findings suggest that miR-143 re-expression vectors or selective agents directed at miR-1

222 ecific Fabpl promoter-driven Cre recombinase expression vector packaged into transferrin-coated nanop

223 We cloned the MnSOD gene, sodA, using the expression vector pBAD, overexpressed the product in Esc

224 A full-length Cx43 cloned into mammalian expression vector pCI-neo was used to transfect testes o

225 alpha3(IV) chain was cloned into a mammalian expression vector, pCI-neo, with and without a collagen

226 1 site in the Gateway (Invitrogen) bacterial expression vector pDEST17, necessary for in vitro site-s

227 n inhibiting osteosarcoma, we constructed an expression vector pEGFP-ING4 and transfected the human o

228 C-terminus, were cloned into the recombinant expression vector pET-28a-PelB and auto-induced in Esche

229 aryotic expression plasmid using prokaryotic expression vector (pET-28a(+)) and converted it to the c

230 entiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of mul

231 They are derived from the broad host range expression vector pNSGroE and have several advantages (i

232 strategies for developing recombinant RVs as expression vectors, potentially leading to next-generati

233 tic function by cloning it into a lentiviral expression vector prior to introduction into the human N

234 he liver of C57BL/6 mice using an adenoviral expression vector produced significant decreases in ADMA

235 c cDNAs with a vaccine strain background and expression vector properties.

236 Co-transfection of APOBEC3A with a TRIB3 expression vector reduced nuclear DNA editing whereas si

237 phorbol 12-myristate 13-acetate or an egr-1 expression vector rescued BKS-2 cells from BCR signal-in

238 f SKI-1/S1P-deficient cells with a SKI-1/S1P expression vector restored release of infectious virus (

239 eroidogenic COS-1 cells with P450c17 and p38 expression vectors showed that p38alpha, but not p38beta

240 Transfection of cell lines with ZNHIT3 expression vectors showed that the PEHO syndrome mutant

241 Use of a dominant-negative Smad3 (Smad3 DN) expression vector, Smad3 small interfering RNA, and inhi

242 Using this TMV expression vector, some foreign proteins were expressed

243 and light-chain DNAs into a single mammalian expression vector, starting from DNAs encoding the desir

244 he incorporation of the DNMT1-specific shRNA expression vector, suggesting a growth defect with reduc

245 Expression vector synthesis and purification of the zymo

246 and Nanog in Sf9 cells using the baculovirus expression vector system (BEVS).

247 prompted the development of a bidirectional expression vector system (pLucRLuc) that is capable of m

248 , we have taken advantage of the baculovirus expression vector system for production of endotoxin-fre

249 apsids in insect cells using the baculovirus expression vector system.

250 JAK2 were overproduced using the baculoviral expression vector system.

251 nomechanics, we designed a family of cloning/expression vectors, termed pFS (plasmid for force spectr

252 nduced phenotypes in vivo, we describe siRNA expression vectors that allow coexpression of one or mor

253 Gateway-compatible pIEx-derived baculovirus expression vectors that allow the rapid and cost-effecti

254 Here we have used these features to design expression vectors that can stably express virtually any

255 he roles of PKCbeta1 and beta2, we used cDNA expression vectors that encode wild-type and constitutiv

256 ly, we have generated a series of multi-copy expression vectors that use resistance to nourseothricin

257 on of opsin/iNOS transgene in the CMVSport 6 expression vector, the 4.4 kb Acc65I/Xhol mouse rod opsi

258 however, when treated with FGFR1OP2/wit3.0 -expression vector, the skin wound closure was significan

259 the fragment cloned into other targeting or expression vectors, the multi-cloning sites of three shu

260 clone PCR products directly into destination/expression vectors, thereby bypassing the requirement fo

261 oosing an appropriate ER signal sequence and expression vector, this simple technology can likely be

262 r Huh7 cells transfected with empty or MAT1A expression vector to establish tumors.

263 to enhance cellular uptake and a linearized expression vector to induce enhanced expression of brain

264 human Cu,Zn-SOD gene with TAT in a bacterial expression vector to produce a genetic in-frame His-tagg

265 a lentiviral-based short hairpin RNA (shRNA) expression vector to stably reduce c-Myc expression in a

266 ct genetic approaches were used to construct expression vectors to achieve SAP overexpression, and bo

267 We designed lentiviral re-expression vectors to achieve targeted re-expression of

268 ssing IFN-beta expression, we used transient expression vectors to test the abilities of a diverse co

269 were co-transfected with GAL3ST3 and B3GNT7 expression vectors, transfected cells weakly expressed H

270 e Nurr1 overexpressed SH-SY5Y cells by Nurr1 expression vector transfection rescued the lactacystin-i

271 r recessive null conditions via a viral gene expression vector transferring a cDNA encoding an enzyme

272 Expression vectors used in different biotechnology appli

273 using mixed infections of a wild-type Trpv1 expression vector vTTHR and a nonfunctional 'poreless' T

274 amplified by homology-based cloning, and its expression vector was constructed for transient expressi

275 C1-peptide cloned into the pCI-neo mammalian expression vector was overexpressed in the testis, to be

276 A zinc-inducible human antizyme gene expression vector was transfected into UM1 human oral sq

277 Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in v

278 type and A205T mutant of the STK11/LKB1 gene expression vectors were created and transfected into RPM

279 High throughput-ready expression vectors were developed that permit rapid clon

280 Based on these findings, dual gene expression vectors were generated, producing an optimal

281 ere introduced into the DHBV genome, genomic expression vectors were transfected into cells which sup

282 oral regulation, Cre/loxP-mediated inducible expression vectors were used in combination with a vecto

283 ide modulator of splicing (bombesin) and SRp expression vectors were used to modulate SRp levels and

284 scFv antibodies (after transfer to mammalian expression vectors) were screened for viability in a neu

285 -viral transfection of a single multiprotein expression vector, which comprises the coding sequences

286 y CD4(+)CD25(-)FOXP3(-) T cells with a FOXP3 expression vector, which resulted in prompt production o

287 commercially available fluorochromes to DNA expression vectors while retaining biological function.

288 It is proposed that this new transient expression vector will be a useful tool for expressing r

289 The optimized FIV-based RNAi expression vectors will find broad use given the extensi

290 me of human BCO1 was cloned into the pET-28b expression vector with a C-terminal polyhistidine tag, a

291 We design a new expression vector with a CAG promoter to detect the EGFP

292 promoter, and that cotransfection of an IRF8 expression vector with an Mdm2 reporter construct stimul

293 Its gene was cloned into an expression vector with an N-terminal polyhistidine tag,

294 ore, the hypoxia/hepatoma dual specific gene expression vector with the Epo enhancer and AFP promoter

295 To test this, we constructed new expression vectors with an artificially synthesized NTR

296 (family Togaviridae) have been developed as expression vectors with demonstrated potential in vaccin

297 AT/enhancer binding protein beta (C/EBPbeta) expression vectors with or without resistin.

298 modules and transfer of assembled arrays to expression vectors without the need for specialized know

299 e transferred from the phagemid to different expression vectors without the need to amplify them by P

300 infection of cells transfected with an ICP22 expression vector yielded ICP22 protein that was modifie