ライフサイエンスコーパス: fMet

1 fMet-Leu-Phe triggered association of a cytosolic NADPH

2 responses to the neutrophil-activating agent fMet-Leu-Phe.

3 rophils were present, the neutrophil agonist fMet-Leu-Phe triggered calcium signaling in Fura-2-loade

4 ion in phorbol 12-myristate 13-acetate - and fMet-Leu-Phe-stimulated COS(phox) cells.

5 l transduction pathways that mediate C5a and fMet-Leu-Phe (fMLP)-induced pertussis toxin (PTx)-sensit

6 ic HL-60 cells, T leukemic MOLT-4 cells, and fMet-Leu-Phe-activated, but not resting, human neutrophi

7 joining, initiation factor dissociation and fMet-tRNA(fMet) positioning during formation of the 70S

8 ed that the ribosome, initiation factors and fMet-tRNA(fMet) can acquire different conformations in t

9 ents to the ribosome, initiation factors and fMet-tRNA(fMet) that occur during maturation of a 70S in

10 apid 50S subunit joining involves a GTP- and fMet-tRNA(fMet)-dependent "activation" of IF2, but a lac

11 e intensities of fluorophore-labeled IF2 and fMet-tRNA(f)(Met).

12 n intensities of fluorophore-labeled IF2 and fMet-tRNA(fMet) to determine the effects on both 30SIC f

13 ormational changes of ribosome-bound IF2 and fMet-tRNA(fMet), which are monitored by spectral changes

14 hanges of fluorescent derivatives of IF2 and fMet-tRNA(fMet).

15 ild-type 70S complex with lambdacI lmRNA and fMet-tRNA(fMet).

16 tion of a binary complex between IF2(mt) and fMet-tRNA that may play an important role in mitochondri

17 contacts the 30S and 50S subunits as well as fMet-tRNA(fMet).

18 -tRNA whereas the fVal-tRNA bound as well as fMet-tRNA.

19 at there was significant competition between fMet-ethyl ester formation and release in all three rele

20 ndrial 28S subunits have the ability to bind fMet-tRNA in the absence of mRNA.

21 essenger via interaction with P-site binding fMet-tRNAfMet.

22 Activation of the respiratory burst by fMet-Leu-Phe was optimal at pH 7.2, but was significantl

23 oss-phosphorylated and cross-desensitized by fMet-Leu-Phe, C5a, and IL-8.

24 ch a single site, the P site, is occupied by fMet-tRNAfMet as directed by an AUG codon containing mRN

25 hese data indicate that activation of p38 by fMet-Leu-Phe and lipopolysaccharide involve different me

26 N-terminal part of PSMalpha2 was replaced by fMet-Ile-Phe-Leu (an FPR1-selective peptide agonist) pot

27 he release of leukotriene C(4) stimulated by fMet-Leu-Phe in IL-5-primed eosinophils.

28 ve inhibition of O-2 generation triggered by fMet-Leu-Phe, immune complexes, or phorbol myristate ace

29 g to programmed ribosomal complexes carrying fMet-tRNA in the P site.

30 trophils stimulated with the chemoattractant fMet-Leu-Phe (fMLP) are known to exhibit rapid activatio

31 L-selectin receptor, or the chemoattractant fMet-Leu-Phe (fMLP), platelet-activating factor, leukotr

32 The chemoattractant fMet-Leu-Phe and the phorbol ester phorbol myristate ace

33 We have reported that the chemoattractant, fMet-Leu-Phe (fMLP), induces the activation of NF-kappaB

34 NAs requires a SD sequence, the start codon, fMet-tRNA(fMet), and the GTP bound form of initiation fa

35 onyl-tRNA formyltransferase, lack detectable fMet-tRNAfMet but exhibit normal mitochondrial function

36 ) release, changes conformation facilitating fMet-tRNA(i)(fMet) accommodation into the P site and tra

37 These are 1.8, 3.5, and 10.5 microM for fMet-tRNA, fVal-tRNA, and fGln-tRNA, respectively.

38 bosomes, exhibiting a 50-fold preference for fMet-tRNA over Met-tRNA in this assay.

39 from tRNA, explaining how IF2 makes way for fMet-tRNA(i)(fMet) accommodation into the P site.

40 initiator tRNAs that carry formylmethionine (fMet), formylglutamine (fGln), or formylvaline (fVal).

41 the level of circulating N-formylmethionine (fMet), which initiates mitochondrial protein translation

42 absence of the N-terminal formylmethionine (fMet), or conversion of the sulfur in this fMet to the s

43 odel of IF2 activation that reveals how GTP, fMet-tRNA(fMet), and specific structural elements of IF2

44 tor transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece of messenger RNA (

45 anges conformation facilitating fMet-tRNA(i)(fMet) accommodation into the P site and transition of th

46 xplaining how IF2 makes way for fMet-tRNA(i)(fMet) accommodation into the P site.

47 bosome in complex with the initiator tRNA(i)(fMet) and a short mRNA.

48 s the proper positioning of the fMet-tRNA(i)(fMet) for the formation of the first peptide bond during

49 RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece of messenger RNA (mRNA) at a re

50 ) holds initiator transfer RNA (fMet-tRNA(i)(fMet)) in a specific orientation in the peptidyl (P) sit

51 involved in neutrophil activation, including fMet-Leu-Phe (fMLP), platelet-activating factor (PAF), g

52 of eosinophils, chemotactic agents including fMet-Leu-Phe, IL-8, and RANTES, promote vigorous transie

53 In a further 11,966 individuals, fMet levels contributed to all-cause mortality and the d

54 nitiation factors, and a dedicated initiator fMet-tRNA(fMet) bind the small (30S) ribosomal subunit.

55 by interfering with the binding of initiator fMet-tRNA(i)(Met) to the ribosomal peptidyltransferase P

56 ed Phe-tRNA(Phe), an analog of the initiator fMet-tRNA(Met), enhanced the population of complexes tha

57 that oxazolidinones interfere with initiator fMet-tRNA binding to the P-site of the ribosomal peptidy

58 mammalian IF2(mt) in complex with initiator fMet-tRNA(iMet) and the eubacterial ribosome.

59 the presence of an aminoacylated initiator, fMet-tRNA(fMet), and IF2 in the GTP-bound state.

60 show that the N terminus of S105 retains its fMet residue but that the N terminus of S107 is fully de

61 replace yeast IF-2(mt) in strains that lack fMet-tRNA which suggests that this paradigm may extend t

62 Chemoattractants like fMet-Leu-Phe (fMLP) induce neutrophils to polarize with

63 same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visuali

64 In human cytoplasmic hybrid (cybrid) lines, fMet modulated both mitochondrial and cytosolic proteins

65 rt peptides bearing N-formylated methionine (fMet) that are characteristic of protein synthesis in ba

66 d on its deletion, proper N-formyl-methionyl(fMet)-tRNA(fMet) positioning and efficient transpeptidat

67 ity of bovine IF-2(mt) to bind mitochondrial fMet-tRNA.

68 a 25-fold greater affinity for mitochondrial fMet-tRNA than Met-tRNA, using either the native mitocho

69 al 55S ribosomes in the presence of IF2(mt), fMet-tRNA and poly(A,U,G).

70 ramework of the crystal structure of the MTF.fMet-tRNA complex published recently.

71 as seen in the crystal structure of the MTF.fMet-tRNA(fMet) complex.

72 critical roles of the first 3 aa linked to N-fMet as well as the C terminus of PSMalpha2 in promoting

73 38 in response to lipopolysaccharide but not fMet-Leu-Phe.

74 In spite of the absence of fMet-tRNA(fMet), the mutant strains exhibited normal mit

75 oop of 16S rRNA or the extended anticodon of fMet-tRNA.

76 dissociation and in promoting the binding of fMet-tRNA to E. coli ribosomes.

77 Full-length IF3(mt) reduces the binding of fMet-tRNA to the 28S subunit in the absence of mRNA.

78 nit association, recruitment, and binding of fMet-tRNA to the ribosomal P-site and initiation dipepti

79 of IF3(mt) to reduce the level of binding of fMet-tRNA to the ribosome in the absence of mRNA.

80 Specific binding of fMet-tRNA(fMet) in the peptidyl (P) site is mediated by

81 70 S formation, but allows normal binding of fMet-tRNA(fMet)(prf20) to the P-site.

82 of translation, inducing the dissociation of fMet-tRNA(fMet) from the 30 S initiation complexes (30SI

83 of EF-P to enhance the rate of formation of fMet-Lys or fMet-Phe, indicating that the role of EF-P i

84 errin release from the secondary granules of fMet-Leu-Phe-activated PMNs was significantly lower at p

85 28S subunits also bind mRNA independently of fMet-tRNA or added initiation factors.

86 IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel o

87 f the 5'-AUG is dependent on the presence of fMet-tRNA and is enhanced by the presence of the mitocho

88 In addition to the removal of fMet, removal of the next two amino acids also resulted

89 n the buffer condition used, whereas that of fMet-tRNAfMet remains the same in both buffer conditions

90 suppresses the respiratory burst of not only fMet-Leu-Phe but also phorbol 12-myristate 13-acetate-st

91 in response to phorbol myristate acetate or fMet-Leu-Phe was reduced in beta-PKC-depleted cells.

92 tion with phorbol 12-myristate 13-acetate or fMet-Leu-Phe, p40(phox) translocated to plasma membrane

93 enhance the rate of formation of fMet-Lys or fMet-Phe, indicating that the role of EF-P is not to spe

94 In contrast, the binding of fVal-tRNA or fMet-tRNA was not affected much by the addition of IF2.

95 Either deacylated tRNAfMet or fMet-tRNAfMet were bound to the 70 S ribosomes, which we

96 Peak fMet-Leu-Phe-induced Ca2+ levels were significantly high

97 here that the prototypic chemotactic peptide fMet-Leu-Phe (fMLF) stimulates the activation of nuclear

98 of receptors include the chemotactic peptide fMet-Leu-Phe, lipoxin A(4), serum amyloid A and beta-amy

99 e cells are treated with chemotactic peptide fMet-Leu-Phe.

100 n complex bound to S. aureus-derived peptide fMet-Ile-Phe-Leu (fMIFL) and E. coli-derived peptide fMe

101 -Phe-Leu (fMIFL) and E. coli-derived peptide fMet-Leu-Phe (fMLF).

102 d not migrate toward the formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albica

103 t affect stable 70 S formation, but perturbs fMet-tRNA(fMet) positioning in the P-site.

104 osomal subunit to an initiator transfer RNA (fMet-tRNA(fMet))-containing 30S ribosomal initiation com

105 factor 2 (IF2) holds initiator transfer RNA (fMet-tRNA(i)(fMet)) in a specific orientation in the pep

106 ndrial initiation factor 2 (IF2(mt)), [(35)S]fMet-tRNA, and either poly(A,U,G) or an in vitro transcr

107 el electrophoresis systems that can separate fMet-tRNA(fMet), Met-tRNA(fMet), and tRNA(fMet) shows th

108 kely forwarded from IF2-G2 to the C-terminal fMet-tRNA binding domain (IF2-C2) because the connected

109 The fGln-tRNA bound less well than fMet-tRNA whereas the fVal-tRNA bound as well as fMet-tR

110 We previously demonstrated that fMet-Leu-Phe (fMLP) stimulates NF-kappaB activation, and

111 Together, these findings indicate that fMet plays a key role in common age-related disease thro

112 coli 70S initiation complexes containing the fMet-tRNA(fMet) M1 variant paired to the noncanonical CU

113 P) site is mediated by the inspection of the fMet moiety by initiation factor IF2 and of three conser

114 IF3(mt) promotes the dissociation of the fMet-tRNA bound in the absence of mRNA.

115 ven slight changes to the recognition of the fMet-tRNA(fMet) anticodon stem by the ribosome can impac

116 -P facilitates the proper positioning of the fMet-tRNA(i)(fMet) for the formation of the first peptid

117 egron pathway, the Pro/N-degron pathway, the fMet/N-degron pathway, and the newly named, in this pers

118 d extended base pairing interaction with the fMet-tRNA anticodon loop, suggesting that this interacti

119 (fMet), or conversion of the sulfur in this fMet to the sulfoxide, resulted in a decrease in LH1 for

120 igration in response to these agonists or to fMet-Leu-Phe occurs only after exposure to differentiati

121 ed equivalent amounts of O(2) in response to fMet-Leu-Phe and phorbol myristate acetate.

122 Boyden chamber, the chemotactic response to fMet-Leu-Phe was maximal at pH 7.2.

123 e accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recogni

124 quire a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation.

125 uses a formylated initiator methionyl-tRNA (fMet-tRNA(f)(Met)).

126 (ICs) that carry an N-formyl-methionyl-tRNA (fMet-tRNA(fMet)).

127 quire a formylated initiator methionyl-tRNA (fMet-tRNAfMet) in a process involving initiation factor

128 vealed that in vitro formation of a 30S-tRNA(fMet)-mRNA ternary complex was inhibited unless a 5' del

129 tor tRNAs, N-acetyl-aminoacyl-tRNAs and tRNA(fMet) dissociated from the P site at a similar low rate,

130 te fMet-tRNA(fMet), Met-tRNA(fMet), and tRNA(fMet) shows that there is no formylation in vivo of the

131 on weakens A-minor interactions between tRNA(fMet) and 16S nucleotides A1339 and G1338, with IF2 stre

132 C40 pair of tRNA(fMet) with C-G (called tRNA(fMet) M1) reduces discrimination against the noncanonica

133 planting identity elements into E. coli tRNA(fMet) , we have engineered an orthogonal initiator tRNA

134 position 37 of an ectopically expressed tRNA(fMet).

135 letion, proper N-formyl-methionyl(fMet)-tRNA(fMet) positioning and efficient transpeptidation are aff

136 changes to the recognition of the fMet-tRNA(fMet) anticodon stem by the ribosome can impact the star

137 factors, and a dedicated initiator fMet-tRNA(fMet) bind the small (30S) ribosomal subunit.

138 e ribosome, initiation factors and fMet-tRNA(fMet) can acquire different conformations in these compl

139 n the crystal structure of the MTF.fMet-tRNA(fMet) complex.

140 tion, inducing the dissociation of fMet-tRNA(fMet) from the 30 S initiation complexes (30SIC) contain

141 Specific binding of fMet-tRNA(fMet) in the peptidyl (P) site is mediated by the inspec

142 nitiation complexes containing the fMet-tRNA(fMet) M1 variant paired to the noncanonical CUG start co

143 initiation factor dissociation and fMet-tRNA(fMet) positioning during formation of the 70S elongation

144 table 70 S formation, but perturbs fMet-tRNA(fMet) positioning in the P-site.

145 e ribosome, initiation factors and fMet-tRNA(fMet) that occur during maturation of a 70S initiation c

146 ies of fluorophore-labeled IF2 and fMet-tRNA(fMet) to determine the effects on both 30SIC formation a

147 tion, but allows normal binding of fMet-tRNA(fMet)(prf20) to the P-site.

148 rmylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation.

149 ation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.

150 unit to an initiator transfer RNA (fMet-tRNA(fMet))-containing 30S ribosomal initiation complex to fo

151 carry an N-formyl-methionyl-tRNA (fMet-tRNA(fMet)).

152 nce of an aminoacylated initiator, fMet-tRNA(fMet), and IF2 in the GTP-bound state.

153 2 activation that reveals how GTP, fMet-tRNA(fMet), and specific structural elements of IF2 drive and

154 es a SD sequence, the start codon, fMet-tRNA(fMet), and the GTP bound form of initiation factor 2 bou

155 The 70SIC contains initiator tRNA, fMet-tRNA(fMet), bound in the P (peptidyl)-site in response to the

156 phoresis systems that can separate fMet-tRNA(fMet), Met-tRNA(fMet), and tRNA(fMet) shows that there i

157 In spite of the absence of fMet-tRNA(fMet), the mutant strains exhibited normal mitochondrial

158 to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation

159 changes of ribosome-bound IF2 and fMet-tRNA(fMet), which are monitored by spectral changes of fluore

160 ubunit joining involves a GTP- and fMet-tRNA(fMet)-dependent "activation" of IF2, but a lack of data

161 0S complex with lambdacI lmRNA and fMet-tRNA(fMet).

162 fluorescent derivatives of IF2 and fMet-tRNA(fMet).

163 he 30S and 50S subunits as well as fMet-tRNA(fMet).

164 that these interactions play a role in tRNA(fMet) discrimination by IF3.

165 for the vital need of the 3GC pairs in tRNA(fMet) for its function in Escherichia coli.

166 t the 3GC pairs play a critical role in tRNA(fMet) retention in ribosome during the conformational ch

167 n is less dependent on the 3GC pairs in tRNA(fMet).

168 ond, the models reconcile how initiator tRNA(fMet) interacts less strongly with the L1 stalk compared

169 I cleaves each strand of the intronless tRNA(fMet) gene adjacent to the anticodon triplet leaving 3 b

170 study the interactions between the Met-tRNA(fMet) and MTF in solution.

171 on of the initiator methionyl-tRNA (Met-tRNA(fMet)).

172 that can separate fMet-tRNA(fMet), Met-tRNA(fMet), and tRNA(fMet) shows that there is no formylation

173 ive promoter (FP1) located 5' to the mt tRNA(fMet)-RNase P RNA-tRNA(Pro) gene cluster, so that the mi

174 In vivo, the 3GC mutant tRNA(fMet) occurred less abundantly in 70S ribosomes but norm

175 enhanced initiation with the 3GC mutant tRNA(fMet), suggesting that the 70S mode of initiation is les

176 AspRS, was found to bind to noncognate tRNA(fMet) in the presence of Mg(2+).

177 de the sole contact to the G-C pairs of tRNA(fMet) bound to the ribosomal P site.

178 ow yields, deleting redundant copies of tRNA(fMet) from the genome afforded an E. coli strain in whic

179 The binding of tRNA(fMet) to amino-terminal peptides was also observed using

180 Swapping the G30-C40 pair of tRNA(fMet) with C-G (called tRNA(fMet) M1) reduces discrimina

181 A-3' terminus and the anticodon loop of tRNA(fMet), and its tRNA specificity is controlled by these i

182 ranslation by site-specific cleavage of tRNA(fMet).

183 -tRNA binding and suggest that ribosome-tRNA(fMet) interactions are uniquely tuned for tight binding.

184 omoter (SP)which is located between the tRNA(fMet) and RPM1 genes.

185 at least one additional feature of the tRNA(fMet) anticodon stem loop.

186 oup I intron has also been found in the tRNA(fMet) gene of some cyanobacteria but not in plastids, su

187 A self-splicing intron in the tRNA(fMet) gene of Synechocystis PCC 6803, which has been pro

188 e entire anticodon stem and loop of the tRNA(fMet) gene.

189 Conversely, the tRNA(fMet) intron has a sporadic distribution, implying that

190 0S initiation complexes containing this tRNA(fMet) variant paired to the canonical bacterial start co

191 no-terminal peptides bound similarly to tRNA(fMet), whereas little or no binding of polynucleotides,

192 uridine (D) stem of the initiator tRNA (tRNA(fMet)).

193 coupled from the expression of upstream tRNA(fMet) gene, and that RPM1 might be independently transcr

194 The 70SIC contains initiator tRNA, fMet-tRNA(fMet), bound in the P (peptidyl)-site in respo

195 of binding affinities of various fAA-tRNAs (fMet-, fGln-, fVal-, fIle-, and fPhe-tRNAs) to IF2 using

196 d formation with the N-formyl group and with fMet, a model supported by MD simulation and functional

197 to mount a rise in Ca2+ when challenged with fMet-Leu-Phe, they increase Ca2+ in response to P2U agon

198 th the fluorophore-Met-tRNA(f) compared with fMet-tRNA(f) with pyrene having the least and eosin the

199 F-2(mt) responsible for the interaction with fMet-tRNA was mapped to the C2 sub-domain of domain VI o

200 The ribosomal reaction of puromycin with fMet-tRNA proceeds 3 x 107-fold more rapidly, with a sec