ライフサイエンスコーパス: farnesylate

1 s mutated to SSIM, a sequence that cannot be farnesylated.

2 es not undergo cleavage and, indeed, remains farnesylated.

3 F2 when it was geranylgeranylated instead of farnesylated.

4 Both were farnesylated.

5 ts that, like Ras proteins, RhoE is normally farnesylated.

6 yl diphosphate, GST-lHDAg was preferentially farnesylated.

7 COS cells demonstrated that the protein was farnesylated.

8 with nonprenylated GTPases that will become farnesylated.

9 cits disease phenotypes whether or not it is farnesylated.

10 n and degradation of Cenp-F require it to be farnesylated [2].

11 of Nm23 proteins were found to contain more farnesylated 46- and 24-kDa proteins than did control ce

12 We examined hRCE1 activity using farnesylated 9 aa peptides with the core sequence, KSKTK

13 used by a mutation in LMNA that produces the farnesylated aberrant lamin A protein, progerin.

14 me caused by a mutation in LMNA yielding the farnesylated aberrant protein progerin.

15 While retinal transducin is farnesylated, all other gamma subunits are modified by g

16 In this study, farnesylated analogs corresponding to the second O-methy

17 The synthesis of two farnesylated analogs of intermediates in the ubiquinone

18 In vitro methyltransferase assays using farnesylated analogues of intermediates in the coenzyme

19 nge is found with various geranylgeranylated/farnesylated analogues.

20 yses showed that like Ras, Rheb proteins are farnesylated and are sensitive to farnesyltransferase in

21 All Ras isoforms are farnesylated and carboxyl-methylated at the terminal cys

22 We have employed properly farnesylated and carboxymethylated KRAS4B in lipid monol

23 roteins or suggesting distinct functions for farnesylated and for geranylgeranylated K-Ras, which is

24 The cysteine 192 RhoB mutant is farnesylated and geranylgeranylated as efficiently as wi

25 ariety of prenylated CaaX peptides including farnesylated and geranylgeranylated forms of human Ha-Ra

26 YopT also cleaves both farnesylated and geranylgeranylated forms of RhoA.

27 This issue is complex because farnesylated and geranylgeranylated forms of RhoB (RhoB-

28 Because K-Ras can be both farnesylated and geranylgeranylated it is not known whet

29 ecombinant hRCE1 so produced recognized both farnesylated and geranylgeranylated proteins as substrat

30 roteins such as Ras and RhoB, which are both farnesylated and involved in cell growth control.

31 aney nickel revealed that the enzyme is only farnesylated and is not geranylgeranylated.

32 the C-terminal amino acids together with the farnesylated and methylated C185 of KRAS4b that binds ti

33 njected into oocytes, a nonpalmitoylated but farnesylated and methylated mutant mislocalizes to the c

34 Prelamin A is farnesylated and methylated on the cysteine residue of a

35 the final processing step does not occur, a farnesylated and methylated prelamin A accumulates in ce

36 Chemical shift mapping studies of farnesylated and non-farnesylated forms of H-Ras with th

37 only farnesylated, like K-Ras4B; in state 2, farnesylated and palmitoylated, like N-Ras.

38 ciation with the plasma membrane through its farnesylated and positively charged C-terminal hypervari

39 known that membrane attachment involves the farnesylated and poylcationic C-terminus of the protein.

40 ng yeast, pheromones produced by a-cells are farnesylated and secreted in a signal peptide-independen

41 ells, prelamin A is a "CAAX protein" that is farnesylated and then processed further to generate matu

42 H-terminal mutants of Rac1 and Rac3 that are farnesylated and used them to characterize Rac1 and Rac3

43 rboxyl-terminal mutants that are exclusively farnesylated and verified that they retain the subcellul

44 rder to assess the relative contributions of farnesylated and/or geranylgeranylated proteins on cell

45 ot distinguish between contributions of C15 (farnesylated) and C20 (geranylgeranylated) species.

46 Some CaaX tetrapeptide inhibitors are not farnesylated, and are more effective inhibitors than far

47 of L-778,123, and a mixture of unprocessed, farnesylated, and geranylgeranylated-Ki4B-Ras in cells t

48 The C589S mutant of hGBP-1, which cannot be farnesylated, and the previously uncharacterized R48P mu

49 in vehicle-treated PSN-1 cells, a mixture of farnesylated- and geranylgeranylated-Ki4B-Ras in cells t

50 This progerin was farnesylated, as judged by metabolic labeling studies.

51 nusual among G(gamma) subunits because it is farnesylated at cysteine 107 and has the potential to be

52 ncated prelamin A, called progerin, which is farnesylated at its carboxyl terminus.

53 We demonstrate that both forms are farnesylated at the carboxyl-terminal cysteine of the CC

54 Ras produced in bacteria as well as in vitro farnesylated bacterial H-Ras are not substrates for PPT

55 CVFM and L-739,750 are not farnesylated, because these inhibitors bind in a conform

56 from binding of a remarkable combination of farnesylated bilins (recently identified, tentative heme

57 dynamic fashion by palmitate, without being farnesylated; bound to membranes modestly (40% as well a

58 the synthesis of a mutant prelamin A that is farnesylated but cannot be further processed to mature l

59 gether, this raises the possibility that the farnesylated but nonpalmitoylated state 1, like K-Ras4B,

60 The majority of the pool consists of farnesylated, but not palmitoylated, N-Ras that is assoc

61 brary also contained 85 peptides that can be farnesylated by FTase only under single-turnover (STO) c

62 etes in both cases but is almost exclusively farnesylated by PFT in the presence of the lamin B pepti

63 MceA is farnesylated by the host cell; however, this does not im

64 e have shown by high-resolution NMR that the farnesylated C-terminal peptide of Gtgamma(60-71), DKNPF

65 It contains a polybasic farnesylated C-terminus that is required for the correct

66 o microdomains are mediated by its polybasic farnesylated C-terminus.

67 Ste24p and Rce1p cleave the C-AAX bond of farnesylated CAAX box proteins.

68 per extremities, resembling mice lacking the farnesylated CAAX protein prelamin A.

69 ated, and are more effective inhibitors than farnesylated CaaX tetrapeptides.

70 s yielded a functional enzyme that correctly farnesylated CaaX-motif-containing peptides.

71 6 delta subunit (PDE6delta) shuttles several farnesylated cargos between membranes.

72 the localization to the kinetochores of two farnesylated centromeric proteins, CENP-E and CENP-F.

73 RAB28 is a farnesylated, ciliary G-protein.

74 udied due to the finding that Ras protein is farnesylated coupled with the observation that mutant fo

75 pidly and was monitored by the appearance of farnesylated CTA1-CVIM in the detergent phase of cell ex

76 at cleaves an intact tripeptide, VIS, from S-farnesylated-CVIS tetrapeptide.

77 tudies revealed high affinity binding of the farnesylated-Cys probe to the FKBP-like domain of AIPL1,

78 l of the final 18 amino acids, including the farnesylated cysteine residue.

79 oylation sites of the N-Ras peptide (the non-farnesylated cysteine) or H-Ras protein (cysteines 181 a

80 idues at the -1 and -3 positions relative to farnesylated cysteine.

81 e (Icmt)-mediated carboxylmethylation of the farnesylated cysteine.

82 or possesses the typical carboxyl-terminal S-farnesylated, cysteine methyl ester and that farnesylati

83 and farnesyl modified dansyl-GCVIM but only farnesylated dansyl-GCVLS.

84 We report here that membrane association of farnesylated Delta50 lamin A tail domains requires calci

85 cleavage step, causing the protein to remain farnesylated, displayed identical localization patterns

86 xpected, non-farnesylated prelamin A and non-farnesylated DNAJA1 accumulated in Fntb-deficient kerati

87 Based on our previous finding that farnesylated DNAJA1 is a crucial chaperone in maintainin

88 ioactive molecules that includes a-factor, a farnesylated dodecapeptide, involved in the mating of Sa

89 in Saccharomyces cerevisiae) or C-terminally farnesylated (e.g., a-factor in S. cerevisiae).

90 rate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo.

91 nhanced green fluorescent protein (EGFP) and farnesylated EGFP (EGFPf) mouse reporter strains to visu

92 greater than that obtained with the original farnesylated EGFP construct.

93 We quantified DPANs in mice expressing the farnesylated enhanced green fluorescent protein (EGFPf)

94 We use mice engineered to express the farnesylated enhanced green fluorescent protein (EGFPf)

95 otein nuDsRed2 and the plasma membrane-bound farnesylated enhanced green fluorescent protein in cultu

96 RhoB is both farnesylated (F) and geranylgeranylated (GG), and RhoB-F

97 on-dependent catalytic activities and can be farnesylated for membrane attachment, oxidized, and trun

98 Progeria (HGPS) is caused by a truncated and farnesylated form of Lamin A.

99 arnesylated prelamin A, comigrating with the farnesylated form of prelamin A that accumulates in ZMPS

100 t lopinavir leads to the accumulation of the farnesylated form of prelamin A.

101 ead to the accumulation of a lipid-modified (farnesylated) form of prelamin A, a protein that contrib

102 hift mapping studies of farnesylated and non-farnesylated forms of H-Ras with the Raf-CRD show that t

103 Moreover, a plasma membrane bound, farnesylated Fyn construct, G2A,C3S-FynKRas, was not enr

104 The farnesylated gamma(11) subunit is highly expressed in he

105 btype-dependent and is more effective toward farnesylated Ggamma types.

106 Endomembrane localization of farnesylated Ggamma(1), but not geranylgeranylated Ggamm

107 essing of two other prenylated proteins, the farnesylated Ggamma1 subunit of transducin and geranylge

108 By expressing farnesylated, GGTI-resistant RalA and RalB in Cos7 cells

109 ck-in mouse model in which an axonal tracer, farnesylated green fluorescence protein (GFP), is expres

110 tor encoding Cre recombinase (Cre)-regulated farnesylated green fluorescent protein (GFPf ) into the

111 inding assays indicate that both recombinant farnesylated GRK1 and geranylgeranylated GRK7 co-precipi

112 ) simulations, we observed that in solution, farnesylated guanosine 5'-diphosphate (GDP)-bound K-Ras4

113 motif (Ras-CVLS) as a substrate but also can farnesylate H-ras in which the CaaX motif is altered to

114 Non-farnesylated H-Ras produced in bacteria as well as in vi

115 effects of L744,832 correlated with loss of farnesylated H-Ras protein and a marked reduction in tra

116 Ras (residues 1-185) and full-length (1-189) farnesylated H-Ras.

117 cked the capacity to proteolytically process farnesylated Ha-Ras, N-Ras, and Ki-Ras or geranylgeranyl

118 hanges and lamellipodia than were induced by farnesylated Ha-Ras61L.

119 he presence of HAS alters the total level of farnesylated hemes, providing further evidence that HOS

120 as, farnesyl-N-Ras, farnesyl-Ha-Ras, and the farnesylated heterotrimeric G protein Ggamma1 subunit, a

121 The farnesylated hGBP1 (hGBP1(fn)) retains many characterist

122 Here, we show how the GTPase cycle of farnesylated hGBP1 (hGBP1F) regulates its self-assembly

123 We showed that hSpindly is farnesylated in vivo and farnesylation is essential for

124 Indeed, we have found that RhoE in farnesylated in vivo and that this modification is requi

125 hown that under normal conditions, Ki-Ras is farnesylated in vivo and when cells are treated with the

126 came more responsive to Ras-GRF2 when it was farnesylated instead of geranylgeranylated.

127 The large form (HDAg-L) is farnesylated, is expressed only at later times via RNA e

128 moval of the three C-terminal amino acids of farnesylated K-Ras with the specific endoprotease Rce1p

129 s.GTP, but not non-farnesylated K-Ras.GTP or farnesylated K-Ras.GDP, was able to activate c-Raf-1 to

130 ombinant farnesylated K-Ras.GTP, but not non-farnesylated K-Ras.GTP or farnesylated K-Ras.GDP, was ab

131 ired for activation, as purified recombinant farnesylated K-Ras.GTP, but not non-farnesylated K-Ras.G

132 and finally the membrane binding of released farnesylated K-Ras4B that leads to K-Ras4B-enriched micr

133 geneous membranes, resulting in a release of farnesylated K-Ras4B upon membrane contact.

134 sis of Ki-Ras prenylation in vivo found only farnesylated Ki-Ras.

135 We find primarily farnesylated-Ki4B-Ras in vehicle-treated PSN-1 cells, a

136 Membrane anchoring of farnesylated KRAS is critical for activation of RAF kina

137 Similarly, Cd-FPT can farnesylate leucine-terminated peptides.

138 In state 1, K-Ras4A is only farnesylated, like K-Ras4B; in state 2, farnesylated and

139 f this processing results in accumulation of farnesylated, membrane-associated prelamin A.

140 address this question, we studied INPP5A, a farnesylated, membrane-bound inositol polyphosphate 5-ph

141 as are not substrates for PPT nor is the non-farnesylated, methylated N-Ras peptide.

142 Crystal form I reveals structural details of farnesylated-methylated KRAS4b binding to PDEdelta, and

143 We solved structures of human farnesylated-methylated KRAS4b in complex with PDEdelta

144 form II, we see the full-length structure of farnesylated-methylated KRAS4b, including the hypervaria

145 by the Ggamma8 gene with an acetylated and a farnesylated-methylated N- and C-terminus, respectively.

146 The photoreceptor G protein, transducin, is farnesylated/methylated at this position.

147 Expression of a stably farnesylated mutant of lamin A phenocopied the immediate

148 e attributes of the dogmatic FTase that only farnesylates non-leucine-ending CAAX substrates and does

149 The ram1p-74D FTase farnesylated only Ras-CIIS and not Ras-CII(M,L), and it

150 ase" responsible for cleavage of prenylated (farnesylated or geranylgeranylated) substrates.

151 y seen in vitro by displaying an increase in farnesylated p21(ras) from 40% of total cellular Ras in

152 nsferase (FTase) and augments the amounts of farnesylated p21.

153 he alpha-subunit of FTase and the amounts of farnesylated p21.

154 AIPL1 has been shown to bind the farnesylated PDE6A subunit.

155 terminal FKBP-like domain of AIPL1 binds the farnesylated PDE6alpha subunit through direct interactio

156 omplex indicates that the sulfur atom of the farnesylated peptide also coordinates the metal.

157 SKOV3) cells with an NDBF-caged version of a farnesylated peptide followed by UV irradiation resulted

158 he GGPP binding mode with the binding of the farnesylated peptide product suggests that the bulkier i

159 Examination of the binding of a farnesylated peptide product to free enzyme revealed a b

160 several isoprenoid analogues, GGPP, and the farnesylated peptide product using a combination of bioc

161 strate was provided, the enzyme released the farnesylated peptide product with rates comparable with

162 quence for Kemptide in tandem, we designed a farnesylated peptide whose localization can dramatically

163 We demonstrated that farnesylated peptides are more strongly retained by reve

164 nce of this post-translational modification, farnesylated peptides have been investigated rarely by m

165 of Rce1, whose endopeptidase specificity for farnesylated peptides mimics that of eukaryotic Rce1.

166 ned the liquid chromatographic separation of farnesylated peptides with the help of the multidimensio

167 ivatives (N-acetyl-S-farnesyl-L-cysteine and farnesylated peptides) have been previously shown to hav

168 This allowed for the identification of farnesylated peptides, if spiked into complex peptide sa

169 visiae and C. albicans have transporters for farnesylated peptides, like the a-factor pheromone, whic

170 e inner leaflet of the plasma membrane via a farnesylated polybasic domain; however, the structural d

171 orescent protein variant that is linked to a farnesylated, polybasic peptide corresponding to the K-R

172 Prelamin A is a farnesylated precursor of lamin A, a nuclear lamina prot

173 olytic step in maturation of prelamin A, the farnesylated precursor of the nuclear scaffold protein l

174 efects in the biogenesis of lamin A from its farnesylated precursor, prelamin A, lead to the accumula

175 a protein lamin A is synthesized as a 74-kDa farnesylated precursor.

176 lele (LmnanPLAO) that yields exclusively non-farnesylated prelamin A (and no lamin C).

177 enzyme, lead to accumulation of full-length farnesylated prelamin A and cause related progeroid diso

178 As expected, non-farnesylated prelamin A and non-farnesylated DNAJA1 accu

179 mice provide a model to study the effects of farnesylated prelamin A during physiological aging.

180 We suspected that the non-farnesylated prelamin A in the tissues of these mice wou

181 tic cleavage of carboxy terminal residues of farnesylated prelamin A in two steps to form mature lami

182 We monitored the proteolysis of farnesylated prelamin A peptide by ZMPSTE24 and unexpect

183 Accumulation of the farnesylated prelamin A variant progerin, with an intern

184 ts solely due to the presence of permanently farnesylated prelamin A.

185 bitors, which lead to an accumulation of non-farnesylated prelamin A.

186 gene, resulting in production of a truncated farnesylated-prelamin A protein (progerin).

187 Additionally, stable FTase-farnesylated product complexes were formed using Ras pro

188 In this case, purified farnesylated product was unambiguously identified by ele

189 the enzyme resulted in the formation of the farnesylated product.

190 erated a second knock-in allele yielding non-farnesylated progerin (Lmna(csmHG)) in which the carboxy

191 uent studies showed that mice expressing non-farnesylated progerin (Lmna(nHG/+) mice, in which proger

192 These studies show that the ability of non-farnesylated progerin to elicit disease depends on the c

193 During interphase, irreversibly farnesylated progerin/LADelta50 anchors to the nuclear m

194 The 80-kDa farnesylated protein could be novel and unique to the le

195 ase block by these agents, suggesting that a farnesylated protein may regulate the mitotic check poin

196 be novel and unique to the lens, because no farnesylated protein of this size has been previously re

197 o homogeneity and identify cPLA(2)gamma as a farnesylated protein that undergoes at least three seque

198 In 2 mM CaCl2, the affinity of the farnesylated protein to a synthetic membrane is KD appro

199 tion of tumor cell growth, suggesting that a farnesylated protein(s) exerts a repressive effect on T

200 These results suggest that farnesylated protein(s) may be involved in regulating p5

201 The results demonstrate that a farnesylated protein(s) mediates IL-1beta induction of N

202 These results link a non-Ras farnesylated protein(s) to the negative regulation of Ca

203 and survival in low serum is dependent on a farnesylated protein, as treatment with farnesyltransfer

204 clathrin-coated vesicle ATPase, peroxisomal farnesylated protein, Ena/vasodilator-stimulated phospho

205 y reveals that rod cGMP phosphodiesterase, a farnesylated protein, is absent and cGMP levels are elev

206 uggesting that atorvastatin dually targets a farnesylated protein, such as Rheb, and a geranylgeranyl

207 ndings, we propose that AIPL1 interacts with farnesylated proteins and plays an essential role in pro

208 e modification enhances the lipophilicity of farnesylated proteins and promotes their association wit

209 Some of the most familiar farnesylated proteins belong to the Ras protein superfam

210 y suggests that the depletion of short-lived farnesylated proteins by manumycin suppresses the antiap

211 ells to proceed from G1 to S phase, and that farnesylated proteins do not play an essential role in t

212 proteins RhoA, Rap1, and R-Ras, but not the farnesylated proteins H-Ras and HDJ-2, is inhibited in t

213 and plays an essential role in processing of farnesylated proteins in retina.

214 Furthermore, the distribution of the total farnesylated proteins in zebrafish retina is also affect

215 However, since farnesylated proteins like Ras are also involved in intr

216 taining oncogenic K-Ras4B, implicating other farnesylated proteins or suggesting distinct functions f

217 Likely candidates are farnesylated proteins such as Ras and RhoB, which are bo

218 cytes revealed the differential responses of farnesylated proteins to an FTI.

219 Farnesylated proteins undergo a series of post-translati

220 ex19p and human and Chinese hamster PxF, all farnesylated proteins, as well as hypothetical proteins

221 function as a potential binding partner for farnesylated proteins, thereby leading to a modulation o

222 ces for FTase derived from analysis of known farnesylated proteins, we selected and screened a librar

223 tional lipid modification of H-Ras and other farnesylated proteins.

224 rated that AIPL1 interacts specifically with farnesylated proteins.

225 shows that AIPL1 enhances the processing of farnesylated proteins.

226 in photoreceptors requires interactions with farnesylated proteins.

227 synthetic mimic of the carboxyl terminus of farnesylated proteins.

228 rotenoids, dolichols, coenzyme Q, heme a and farnesylated proteins.

229 ompounds affect cellular processes involving farnesylated proteins.

230 e) have shown that the enzyme preferentially farnesylates proteins ending in CAAX (C = cysteine, A =

231 PRL-1 is a predominantly nuclear, farnesylated PTPase that has been linked to the control

232 Five farnesylated radiolabeled tetrapeptides, Ac-C(F)FM[3H]L,

233 Conversely, farnesylated RalA but not RalB expression renders MiaPaC

234 by GGTI-2417 treatment, we demonstrated that farnesylated RalB, but not RalA, confers resistance to t

235 Furthermore, farnesylated RalB, but not RalA, inhibits the ability of

236 es by decreasing geranylgeranylated RhoA and farnesylated Ras at the plasma membrane.

237 ssing is required for proper localization of farnesylated Ras but not geranygeranylated Rho proteins.

238 he apparent formation of stable complexes of farnesylated Ras protein that can then be optimally trap

239 In MEFs lacking Rce1 or Icmt, farnesylated Ras proteins were mislocalized.

240 o function as potential binding partners for farnesylated Ras proteins.

241 he first physiological function for this new farnesylated Ras superfamily G-protein.

242 We tested the hypothesis that inhibition of farnesylated Ras was causally related to manumycin-induc

243 The ram1p-206DDLF FTase farnesylated Ras-CII(S,M,L) at wild type levels but coul

244 The ram1p-351FSKN FTase farnesylated Ras-CIIS and Ras-CIIM but not Ras-CIIL.

245 th no change in the membrane localization of farnesylated Ras.

246 e to assess their functional conservation in farnesylating Ras and a-factor proteins, which are impor

247 Blocking of Eomes protein transfer using a farnesylated red fluorescent protein (CherryF) also prev

248 Although farnesylated Rheb exhibited partial endoplasmic reticulu

249 We proposed previously that farnesylated Rho proteins are important targets for alte

250 on, summarize findings suggesting a role for farnesylated Rho proteins prompted by studies on RhoB, a

251 Cells treated with FTIs exhibit a loss of farnesylated RhoB but a gain of geranylgeranylated RhoB

252 Immunocytochemistry showed that farnesylated rhodopsin kinase (GRK1) and prenylated rod

253 th a PDEdelta bait that it can interact with farnesylated rhodopsin kinase (GRK1) and that prenylatio

254 The crystal structure of a phosphorylated, farnesylated Rnd3 peptide with 14-3-3 reveals a hydropho

255 farnib inhibited signaling downstream of the farnesylated small G protein Rheb and synergistically en

256 Rheb (Ras homologue enriched in brain) is a farnesylated small GTPase that positively regulates mTOR

257 he geranylgeranylated mutant form of a novel farnesylated small GTPase, SpRheb, is able to suppress t

258 The methodology is applicable to many other farnesylated small GTPases.

259 cycle progression of S. pombe cells and that farnesylated SpRheb is critical in this process.

260 coli cell lysates and synthetically prepared farnesylated-substrate analogs indicate that UbiG methyl

261 smaller GTPases of the Ras/Rab family, it is farnesylated, suggesting it may dock into membranes and

262 combination with our finding that ScRheb is farnesylated, suggests that farnesylation plays a key ro

263 ells with wild-type Ras, implying that other farnesylated targets contribute to FTI action.

264 at regulate the G2/M checkpoint are critical farnesylated targets of FTI action.

265 ransfection studies, is more active with the farnesylated than geranylgeranylated cysteinyl substrate

266 enotype results mainly from the inability to farnesylate the Rheb G-protein, as normal cell cycle pro

267 d abnormal nuclear envelope association of a farnesylated, truncated variant of prelamin A called "pr

268 However, the farnesylated versions of both activated and wild-type Ra

269 Farnesylated versions of Rac1 and Rac3 retain the abilit

270 und that genotype 3 HDAg-L was inefficiently farnesylated when expressed in the absence of the small

271 The 50-kDa protein is shown to be farnesylated, whereas the 22-28-kDa proteins are geranyl

272 , suggesting that the diffusion barriers and farnesylated Ydj1 functioned together to confine protein