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1 oth in the presence and absence of the glial feeder layer.
2 ed the survival of CLL cells on a macrophage feeder layer.
3 n serum-containing medium using a fibroblast feeder layer.
4  pure neuronal cultures deprived of the glia feeder layer.
5 use embryonic fibroblast (iMEF) is used as a feeder layer.
6 n blot; SC clonal growth was measured on 3T3 feeder layers.
7 c cultures in the presence or absence of MSC feeder layers.
8 ed in media with or without serum and/or 3T3 feeder layers.
9 in an undifferentiated state by culturing on feeder layers.
10 animal-derived 'serum replacements' on mouse feeder layers.
11 t mechanisms when neurons are grown on glial feeder layers.
12 oly-L-lysine-coated glass substrates without feeder layers.
13 use yolk sac-derived endothelial cell (C166) feeder layers.
14                    While culture of HMECs on feeder layers abrogates M0 and p16(INK4A) inactivation,
15                        A cardiac mesenchymal feeder layer allows renewal of the isolated progenitor c
16 lips, which are suspended above an astrocyte feeder layer and maintained in serum-free medium.
17 J2 with a growth arrested primary fibroblast feeder layer and using pooled Human Platelet lysate (HPL
18 showed vivid clonal growth on 3T3 fibroblast feeder layers and complete epithelial-mesenchymal transi
19 in the germ line has allowed a separation of feeder layers and contaminating testis somatic cells fro
20 er different conditions were seeded onto 3T3 feeder layers and cultured for 12 days.
21  episomal or integrated HPV-16, but required feeder-layer and growth-factor support, were consistentl
22                        In vitro selection of feeder-layer and growth-factor-independent variants even
23 ioned medium from mouse embryonic fibroblast feeder layers, and (ii) direct cell differentiation.
24  cells and their progeny to animal and human feeder layers, and thus the risk of contamination with p
25 ells cultured on bone marrow-derived stromal feeder layers are more resistant to chemotherapy, increa
26 ltivating the telomerase-expressing cells on feeder layers avoids the growth arrest associated with i
27 ) cell lines were derived 25 years ago using feeder-layer-based blastocyst cultures, subsequent effor
28                                    Without a feeder layer but in the presence of hepatocyte growth fa
29   By culturing MGE progenitors on a cortical feeder layer, cell fate analyses suggest that Shh signal
30      Once cell colonies were established REF feeder layers could be replaced with REF conditioned med
31 e of the role of HA in early development and feeder layer cultures of hESCs and the controllability o
32          Previous developments using stromal feeder layers, defined media cocktails, and bioengineeri
33                Cells grown on inserts with a feeder layer developed into confluent monolayers consist
34    Here we show that primary HMECs, grown on feeder layers, do not undergo this growth arrest and can
35 agated indefinitely when placed onto stromal feeder layers engineered to produce IL-7.
36 gation of hESCs on mouse fibroblast or human feeder layers, enzymatic cell removal, and spontaneous d
37 ures from mice lacking nNOS grown on a glial feeder layer fail to respond to neurotrophin-mediated en
38 bal specimens and clonally expanded on a 3T3 feeder layer, followed by subcultivation of holoclones o
39  and obviates the requirement for serum or a feeder layer for maintenance.
40  primary human skin fibroblasts were used as feeder layers for cloning lymphoid cells by limiting dil
41 ore, mouse and human amniocytes can serve as feeder layers for iPS cells and for mouse and human embr
42  limbal stroma when seeded on 3T3 fibroblast feeder layers from P1 to P3.
43                         Mitotically inactive feeder layers have been used previously to support the g
44 kely to require using human serum with human feeder layers, ideally starting with fresh HESC that hav
45 on by culture on a mitomycin C treated STON+ feeder layer in a hepatoblast culture medium consisting
46 ation) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leuke
47  in Matrigel in the absence of a mesenchymal feeder layer, individual cells divided and formed self-o
48 ead angiogenesis assay experiment, FCSC cell feeder layer induced HUVECs to form significantly shorte
49 e primary neuronal cultures grown on a glial feeder layer is significantly less than that of neurons
50   In vitro colony assays with rUCMS cells as feeder layers markedly reduced Mat B III colony size and
51 ouse-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in n
52 pluripotent stem cells requires the use of a feeder layer of cells.
53 udies demonstrated the use of an alternative feeder layer of human amniotic epithelial cells (hAECs)
54  cultures by using a combination of L1 and a feeder layer of human hair follicle-derived mesenchymal
55 gen-coated culture inserts suspended above a feeder layer of myofibroblasts.
56 cosal tissues (ANM cells) were plated onto a feeder layer of newborn rat cortical glia (astrocytes) i
57  Blastocysts were cultured individually on a feeder layer of rat embryonic fibroblasts (REFs) in fibr
58                                              Feeder layers of cerebellar granule cells derived from w
59 bone marrow microenvironment and cultured on feeder layers of CSF-1, DBP-MAF, or DBP-MAF/CSF-1 for co
60 By contrast, mouse EBs cultured alone, or on feeder layers of mouse embryonic fibroblasts or avian no
61 nic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (M
62 rmatogonial behavior similar to that seen on feeder layers of SNL fibroblasts.
63 tent cells without the need for coculture on feeder layers or cell sorting to obtain a highly enriche
64 (hESCs) are routinely cultured on fibroblast feeder layers or in fibroblast-conditioned medium (CM).
65 f these iPS cells on mitotically inactivated feeder layers prepared from the same amniocytes.
66 ddition of neuregulin-1 to cultures on MSC-1 feeder layers resulted in spermatogonial behavior simila
67 ulture conditions consisting of a fibroblast feeder layer, rho-associated coiled coil protein kinase
68 enescence on plastic (S-P) and senescence on feeder layers (S-FL) in HMECs, as it does also in cultur
69 ns cultivated in the absence of an astrocyte feeder layer showed abundant AbetaO binding to dendritic
70                   Serially passaged with 3T3 feeder layer support, the keratinocytes were examined fo
71 nsional collagen gel matrix and a fibroblast feeder layer that supports hepatocytic differentiation f
72 ficient, serum-free culture medium without a feeder layer, that could be used for intra-oral grafting
73                        When cultured without feeder layers, the early lineage markers disappear, and
74 hen cortical neurons are cultured on a glial feeder layer, they do not reach nearly as mature a pheno
75 nd cell mixtures failed to produce a stromal feeder layer to support marrow cell growth in vitro.
76 rly hepatic lineage markers when cultured on feeder layers; under these conditions, neither mature he
77           The presence of a mesenchymal cell feeder layer was essential for supporting hepatocytic di
78 ch passage, clonogenicity on 3T3 fibroblasts feeder layers was compared among progenitor cells remove
79 nic stem cells (hESCs) differentiated on S17 feeder layer were transplanted by intraperitoneal inject
80 ies had no animal-derived products or murine feeder layers, whereas only 1 study had both.
81 e system using fetal bone stromal cells as a feeder layer, which facilitates the survival and growth
82 earch utilises growth arrested murine 3T3 J2 feeder layers, which are not available for use as a GMP
83 experiments use primary embryonic fibroblast feeder layers, which assist in the maintenance of the pl