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1 od at steady state = 300 ppb, per voxel = 45 femtomoles).
2 ificity, and has a limit of detection of 1.5 femtomoles.
3 ion limits were improved to approximately 10 femtomoles.
4 cros with sample consumption on the order of femtomoles.
5 n values ranged from 63.75 attomoles to 1.21 femtomoles.
6 xing time of 8 mus and sample consumption of femtomoles.
9 ethodology capable of quantifying at the low femtomoles/10(6) cells level was necessary to understand
11 ion intensity and quantity from picomole to femtomole amounts by liquid chromatography-electrospray
12 om proteins and cells, and enrichment of sub-femtomole amounts of ADP-ribosylated peptides from cell
13 detection of enriched phosphopeptides in mid-femtomole amounts of alpha-casein digests per MALDI spot
14 have been developed for the determination of femtomole amounts of mycothiol (MSH), the main low-molec
15 thodology to assay development, by detecting femtomole amounts of N-terminal FLAG-bacteria alkaline p
16 noassays, diverse misfolded proteins and sub-femtomole amounts of oligomeric aggregates can be detect
23 metry (TOF-SIMS) has been utilized to detect femtomole and attomole quantities of organic species fro
24 nge from 1 x 10(-2) to 1 x 10(-4) M allowing femtomole and even attomole quantities of material to be
25 izes high sensitivity (detection limit: ~100 femtomole) and reproducibility in the peptide screening.
26 vestigated, very between 85 attomoles and 25 femtomoles, and typical acquisition times are approximat
27 e and lactate concentrations by fractions of femtomoles are detected from just 2000 live cells at a t
28 120 kDa in size present at levels to tens of femtomoles, as compared to the 100 proteins found by sil
30 ography/mass spectrometry method that allows femtomole detection and quantification of anandamide and
32 -power MS analysis with results showing ~7.0 femtomole detection limits and linear spectral response
36 ocked and unblocked beacons offer nanomolar (femtomole) detection limits, response times on the order
39 ith the detection limits of approximately 40 femtomole (for cholic acid) and identification through C
40 molar levels (x10(-12) M, equivalent to ~1.1 femtomoles IsdA) with a dynamic range extending to ~40 n
41 ity of the method was found to be at the low-femtomole level (1.25 ng/band), determined by analyzing
42 determined, with the detection limit in the femtomole level and the calibration range spanning over
45 r enabled us to identify proteins at the low femtomole level in solution-phase digests and at the pic
47 roteins added to serum, this method provides femtomole level sensitivity and reproducible label-free
48 is immunoassay achieves optimal sensitivity (femtomole level) with minimal sample handling and is ame
49 were obtained for all the samples at the low femtomole level, proving that LC-AP-ECD-MS is suitable f
55 DCDB MIBD could facilitate nanoliter-volume, femtomole-level universal detection in applications rang
59 ino acids were separated and detected at low-femtomole levels using a 130-cm-long, 20-microm-i.d., 15
60 a protein kinase A peptide substrate, at low femtomole levels when it was added as a trace marker com
61 (LOQ) of nine standard glycoproteins reached femtomole levels with dynamic ranges spanning 3-4 orders
65 ths after a desalination rinse, resulting in femtomole limits of detection via matrix-assisted laser
68 it fraction were 0.77+/-0.03 nM and 180+/-21 femtomoles/mg protein and 4.3 nM and 1.3 picomoles/mg pr
69 tein), lowest in the neurosensory retina (14 femtomoles/mg protein), and intermediate in the other tw
70 ergic receptors was highest in the iris (440 femtomoles/mg protein), lowest in the neurosensory retin
71 membranes with a Kd = 69.6 pM and Bmax = 80 femtomoles/mg protein; no specific binding was found in
73 l aptamer-based assay capable of detecting 1 femtomole of dNTP, enabling dNTP measurements in virions
75 trate and detect the presence of less than a femtomole of streptavidin in 10 muL of sample using fluo
78 Our new model assay technology can detect femtomoles of biotinylated BSA in less than 2 min and ca
81 er software for the detailed analysis of low femtomoles of N-linked sugars released from glycoprotein
84 cific sensitivity thresholds on the order of femtomoles of proteins and dynamic ranges of over two or
86 le molecular interaction can be studied with femtomoles of solute, and (3) BIRC has the potential to
87 NA) sensor built on this strategy can detect femtomoles of target DNA without employing cumbersome an
88 The assay is complete within 5 min, and <100 femtomoles of target produces color changes observable w
90 ion and quantification of attomoles or a few femtomoles of two (or potentially more) nucleic acid tar
93 magnitude, with detection limits in the low femtomole per milligram of protein level, making this wo
95 f ovalbumin is demonstrated where binding of femtomole protein is differentiable from the background
98 throughput thermal stability measurements of femtomole quantities of CCR5 in detergent and in enginee
99 solution structural information from scarce, femtomole quantities of Moloney murine leukemia virus (M
101 , a recently developed technique to sequence femtomole quantities of polyacrylamide gel-separated pro
102 zation mass spectrometry (ESI-MS) to measure femtomole quantities of proteins in individual pico- to
106 We demonstrate the method on detection of femtomole quantities of untagged oligonucleotides in an
108 e and quantified 12 nucleotide sugars at low femtomole quantities, with linear responses up to four o
109 Limits of detection in the single to tens of femtomole range are reported for selected compounds.
111 cluding nerves and neuronal clusters, in the femtomole range with a limit of detection of 19 fmol.
112 s rapid detection of coat protein in the low-femtomole range, as estimated by titering plaque-forming
113 vantages include inherent sensitivity in the femtomole range, compatibility with collisional activati
114 and with enzyme detection limits in the low femtomole range, supports the utility of this assay syst
115 on (LODs) range from the low attomole to the femtomole range, with 5-hydroxytryptamine (or serotonin
116 own over almost 3 orders of magnitude in the femtomole range, with limits of detection and quantifica
130 cotinamide-dependent enzymes in the nano- to femtomole scale, in alternative enzymatic assays, and in
131 on conditions were optimized down to the low-femtomole sensitivities with the use of an ion trap mass
133 ensors are a surface-sensitive NMR tool with femtomole sensitivity for in situ analysis in catalysis,
135 alysis of proteins/peptides in solution, low femtomole sensitivity has been achieved (attomoles for s
140 ed proteins that are 20-100 times lower (low femtomole) than those reported for previous polymer- or
141 feasibility of the routine quantification of femtomole to attomole amounts of known proteins by immun
143 eparation and identification of catechins at femtomole to low-picomole levels without extraction or s
144 and mass spectrometry (LC-ESI-MS) to detect femtomole to picomole amounts of reporter molecules resu
146 eins by their amino acid sequence at the low-femtomole to subfemtomole sensitivity level is described
148 only 25 ng of total AAV capsid proteins (4.3 femtomole virus particles) were loaded to the autosample
150 olutions as diluted as 1 pM at 450 muL (0.45 femtomole) was achieved in less than 3h, making our appr