ライフサイエンスコーパス: fibrillarin

1 ng the Tudor domain, mediates the binding of fibrillarin.

2 nvolved in rRNA processing and interact with fibrillarin.

3 mice that targets the nucleolar autoantigen fibrillarin.

4 ages resulted in the proteolytic cleavage of fibrillarin.

5 hypermethylation and fail to associate with fibrillarin.

6 in boxes C, C', D, and D' and associate with fibrillarin.

7 of MPP10 arrived at the nucleolus later than fibrillarin.

8 NA) species, the vast majority of which bind fibrillarin.

9 f the NTD itself prevented dimerization with fibrillarin.

10 minal domain of Nop56/58 that interacts with fibrillarin.

11 along with three proteins: L7Ae, Nop5p, and fibrillarin.

12 otein encoded by open reading frame 3), with fibrillarin.

13 olar markers such as B23 (nucleophosmin) and fibrillarin.

14 differences at the cofactor-binding site in fibrillarin.

15 ith Nop58, and the D and D' boxes contacting fibrillarin.

16 NLS-containing proteins Arabidopsis thaliana fibrillarin 1 (AtFib1) and the Nuk6, Nuk7 and Nuk12 cand

17 ies of nucleostemin differed strikingly from fibrillarin (a protein directly involved in rRNA process

18 n prostate cancer, we examined FBL (encoding fibrillarin), a MYC target gene, and report that fibrill

19 nucleolar autoantibody response that targets fibrillarin, a 34-kDa protein component of many small nu

20 Nm modifications in eukaryotes are placed by Fibrillarin, a conserved methyltransferase belonging to

21 ve demonstrated that SMN also interacts with fibrillarin, a highly conserved nucleolar protein that i

22 Some of these bodies lacked fibrillarin, a previously described component of nucleol

23 hout mitosis, it co-localizes with NOP56 and fibrillarin, a putative methyl transferase, only during

24 miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional

25 t H-2s does not program mice to produce anti-fibrillarin Abs in response to nonspecific immune stimul

26 In contrast, mercuric chloride induces anti-fibrillarin Abs only in SJL and other H-2s mice, and not

27 brillarin, the 19-kDa fragment produced anti-fibrillarin Abs with some of the properties of the HgCl2

28 Calorimetry studies of cofactor binding to fibrillarin alone and to fibrillarin-Nop5p binary comple

29 Knockdown of fibrillarin also reduces nucleolar size and extends life

30 strate such as the glutathione S-transferase-fibrillarin amino-terminal fusion protein (GST-GAR), it

31 histone H2A and a glutathione S-transferase-fibrillarin (amino acids 1-148) fusion protein (glutathi

32 o catalyze the in vitro methylation of a GST-fibrillarin (amino acids 1-148) fusion protein (GST-GAR)

33 a cation-pi bridge formed between Tyr-89 of fibrillarin and Arg-169 of Nop5p, although dispensable f

34 cently determined crystal structures of free fibrillarin and fibrillarin-Nop5p-AdoMet tertiary comple

35 e arginine- and glycine-rich domains of both fibrillarin and GAR1 and is defective in SMN mutants fou

36 Fibrillarin and GAR1 are specific markers of the two cla

37 These findings identify fibrillarin and GAR1 as novel interactors of SMN and sug

38 f SMN to the nucleolar periphery and loss of fibrillarin and GAR1 colocalization with SMN in gems.

39 two major families of small nucleolar RNPs, fibrillarin and GAR1, suggesting that SMN may also funct

40 We show that SMN binds directly to fibrillarin and GAR1.

41 homologs of the box C/D snoRNP core proteins fibrillarin and Nop56/58 have also been identified but a

42 F57 associates with snoRNAs independently of fibrillarin and NOP58 proteins and with rRNA in the regi

43 In Archaea, fibrillarin and Nop5p form the core complex of box C/D s

44 tructure of the core protein complex between fibrillarin and Nop5p.

45 harbors nascent pol I transcripts as well as fibrillarin and nucleolin, which function in early phase

46 h antibodies against two nucleolar proteins, fibrillarin and nucleolin.

47 uorescence, MPP10 colocalized with nucleolar fibrillarin and other known nucleolar proteins in interp

48 Fibrillarin and SMN co-immunoprecipitate from HeLa cell

49 autoantibodies (ANoA) and for antibodies to fibrillarin and the U3 snoRNP-specific proteins Mpp10 an

50 nstrate that the SMN complex associates with fibrillarin and with GAR1 in vivo.

51 RGG box region of hnRNP U, with itself, with fibrillarin and with several novel proteins.

52 immunodominant peptides of topoisomerase 1, fibrillarin, and centromere protein A and discovered tha

53 he expression of ribosomal protein genes and fibrillarin, and reduced nascent protein synthesis, indi

54 inding factor [UBF]), processing (nucleolin, fibrillarin, and RNase MRP subunits, Rpp29), and ribosom

55 creased frequencies of anti-topoisomerase I, fibrillarin, and RNP autoantibodies compared with whites

56 eport that the nucleolar RNA proteins Rpp29, fibrillarin, and RPL23a are also components of this H3.3

57 BIG1, nucleolin, U3, the U3-binding protein fibrillarin, and the RNA-binding protein La may exist to

58 s infectious virus than extracts depleted of fibrillarin, another resident of the nucleolus, indicati

59 ations from T. brucei extracts with the anti-fibrillarin antibodies indicated that this trypanosomati

60 on in the Drosophila eye imaginal disc using fibrillarin antibody labeling.

61 duced cell death was associated with loss of fibrillarin antigenicity and modification of the molecul

62 modification of the molecular properties of fibrillarin as revealed by aberrant migration under nonr

63 larin autoantibodies, pointing to unmodified fibrillarin as the B cell Ag and implicating mercury-mod

64 e B cell Ag and implicating mercury-modified fibrillarin as the source of T cell antigenicity.

65 ient, impair the interaction between SMN and fibrillarin (as well as the common snRNP protein SmB).

66 include the nucleolar proteins nucleolin and fibrillarin, as well as the eukaryotic initiation factor

67 Our results indicate that T. brucei has many fibrillarin-associated box C/D snoRNAs with roles in 2'-

68 We report the identification of 17 box C/D fibrillarin-associated small nucleolar RNAs (snoRNAs) fr

69 Most of the fibrillarin-associated snoRNAs can form 10- to 21-nt dup

70 box C, D, C', and D' elements, a hallmark of fibrillarin-associated snoRNAs in eukaryotes.

71 e show that, in addition to U22, seven novel fibrillarin-associated snoRNAs, named U25-U31, are encod

72 ted that this trypanosomatid has at least 30 fibrillarin-associated snoRNAs.

73 mercury reduced immunoprecipitation by anti-fibrillarin autoantibodies, pointing to unmodified fibri

74 We observed that the nucleolar protein fibrillarin began to redistribute by 6 h after oxaliplat

75 associates with the rRNA processing protein fibrillarin but, surprisingly, that processed rRNA produ

76 uclei also resulted in aberrant migration of fibrillarin, but not other nuclear autoantigens.

77 e modification of the molecular structure of fibrillarin by mercury reduced immunoprecipitation by an

78 We previously proposed that replacement of fibrillarin by Nop52 (RRP1/NNP-1) for the binding to p32

79 ount for the enhanced binding of cofactor to fibrillarin by Nop5p.

80 Evidence that BIG1 and nucleolin, but not fibrillarin, can be present with p62 at the nuclear enve

81 nucleolar dense fibrillar component protein fibrillarin closely matched the level of nucleolar assem

82 report the crystal structure of the Nop56/58-fibrillarin complex bound with methylation cofactor, S-a

83 x revealed a symmetric dimer of two Nop56/58-fibrillarin complexes linked by the coiled-coil domains

84 Subsequent binding of the Nop56/58 and fibrillarin core proteins to the L7-sRNA complex further

85 ransferase reaction by bridging the L7Ae and fibrillarin core proteins.

86 RNA knockdown confirmed that methylation is fibrillarin dependent.

87 ibosomal proteins, and the nucleolar protein fibrillarin, dependent on NCL-1.

88 th the accumulation of large precursors, and fibrillarin does not accumulate in nucleoli.

89 2) messenger RNA, a modification conveyed by fibrillarin during erythropoiesis, subsequently reducing

90 g CBs to enter the nucleolus and, along with fibrillarin, exit the nucleus to form viral 'transport-c

91 Knockdown of fibrillarin expression decreases the expression of snoRN

92 eroderma, coupled with the observations that fibrillarin expression is positively linked to collagen

93 e-peptide mutants exhibit small nucleoli and fibrillarin expression, as do long-lived dietary restric

94 d cell cycle factors, the nucleolar proteins fibrillarin (Fb) and Nopp140 (Nopp), the survival motor

95 ues performed a CRISPR screen and identified fibrillarin (FBL) as a new driver in AML leukemogenesis.

96 ethyltransferase, can directly interact with fibrillarin (FBL) to exert its role in translational reg

97 We report that snoRNAs and fibrillarin (FBL, an enzymatic small nucleolar ribonucle

98 ar dynamics and ribosome function, including fibrillarin (FIB-1/FBL) and RPL-11 (RPL11).

99 /D RNP, the affinity of the catalytic module fibrillarin for the substrate-guide helix is dependent o

100 ein via Cajal bodies, relocalization of some fibrillarin from the nucleolus to cytoplasm, and assembl

101 s, myelin basic protein, a fragment of human fibrillarin (GAR) and spliceosomal protein SmB.

102 The archaeal prp and fibrillarin gene homologs were found adjacent to each ot

103 very after photobleaching (FRAP) showed that fibrillarin-GFP reassociates with the NDF and PNBs at ra

104 cepting substrates glutathione S-transferase fibrillarin glycine arginine domain fusion protein or he

105 g a GST fusion with the N-terminal domain of fibrillarin (GST-GAR), myelin basic protein, and recombi

106 Fibrillarin has a conserved methyltransferase fold and e

107 hii (Mj) Nop56/58 with the methyltransferase fibrillarin has been investigated using site-directed mu

108 g recombinant core proteins L7, Nop56/58 and fibrillarin has yielded an RNA:protein enzyme that guide

109 The Nop56/58-fibrillarin heterocomplex is a core protein complex of t

110 The extreme stability of the Nop56/58-fibrillarin heterodimer was confirmed in both chemical a

111 many yeast nucleolar proteins, including the fibrillarin homolog Nop1p, to relocate to the cytoplasm.

112 n (named FibM) was identified as an archaeal fibrillarin homolog.

113 Here we report the crystal structure of the fibrillarin homologue from Methanococcus jannaschii, a h

114 uces autoantibodies to the nucleolar protein fibrillarin in H-2s, but not in H-2b, mice.

115 ly by inhibiting the rRNA methyl-transferase fibrillarin in human cells.

116 vealed that the P20 protein colocalized with fibrillarin in the nucleoli and formed punctate structur

117 s, which abolished the aberrant migration of fibrillarin in the presence of HgCl2.

118 mall nucleolar RNP (snoRNP) proteins, Nop1p (fibrillarin in vertebrates) and Nop58p (also known as No

119 smic relocalization of nucleolin, but not of fibrillarin, in poliovirus-infected cells.

120 nd fib genes raises the possibility of a Prp-fibrillarin interaction in archaea.

121 e 19-kDa fragment, suggesting that a mercury-fibrillarin interaction was not necessary for the unique

122 enesis revealed an unusually strong Nop56/58-fibrillarin interaction.

123 Fibrillarin is a phylogenetically conserved protein esse

124 ts revealed that the major nucleolar protein fibrillarin is coprecipitated in the P20 protein complex

125 ndicate that the interaction between SMN and fibrillarin is direct and salt-stable.

126 how that the glycine/arginine-rich domain of fibrillarin is necessary and sufficient for SMN binding

127 Sm snRNP proteins, interaction with GAR1 and fibrillarin is not enhanced by arginine dimethylation.

128 Further, fibrillarin is overexpressed in PIN lesions induced by M

129 collagen expression in fibroblasts and that fibrillarin is overexpressed in scleroderma fibroblasts,

130 Importantly, fibrillarin is repositioned within the two complexes.

131 illarin), a MYC target gene, and report that fibrillarin is required for proliferation, clonogenic su

132 d, suggesting that binding of Nop5p alone to fibrillarin is sufficient to stabilize the AdoMet-bindin

133 hyltransferase in yeast and demonstrate that fibrillarin is the orthologue enzyme in human cells.

134 Proteolysis of fibrillarin lacking cysteines, and therefore unable to b

135 RNAs (SNORDs) and the 2'-O-methyltransferase fibrillarin lead to Nm modification in the protein-codin

136 panied by decreased nucleolar size and lower fibrillarin levels.

137 denosine deaminase acting on RNA 1 and 2 and fibrillarin negatively influence each other's expression

138 mperature-sensitive mutations found in yeast fibrillarin Nop1 to the Methanococcus homologue structur

139 RNP proteins including the enzymatic subunit fibrillarin/Nop1.

140 h the X. laevis Box C/D RNA binding proteins fibrillarin, Nop56, and Nop58.

141 oteins (snoRNPs) contain four core proteins: fibrillarin, Nop56, Nop58 and 15.5 kDa.

142 box C/D RNP containing three core proteins (fibrillarin, Nop56/58, and L7Ae) and a half-mer box C/D

143 e proteins around a box C/D RNA that include fibrillarin, Nop5p, and L7Ae.

144 crystal structure of Archaeoglobus fulgidus fibrillarin-Nop5p binary complex at 3.5 A.

145 cofactor binding to fibrillarin alone and to fibrillarin-Nop5p binary complex provided further suppor

146 ymmetries in both the box C/D RNA and in the fibrillarin-Nop5p complex are required for efficient cat

147 g one of the two box C/D motifs and a mutant fibrillarin-Nop5p complex deficient in self-association.

148 ric features both in box C/D RNAs and in the fibrillarin-Nop5p complex.

149 d crystal structures of free fibrillarin and fibrillarin-Nop5p-AdoMet tertiary complex revealed large

150 lude alpha tubulin, beta actin, desmoplakin, fibrillarin, nuclear lamin B1, nonmuscle myosin heavy ch

151 sing, including U3 small nucleolar (sno)RNA, fibrillarin, nucleolin, and proteins B23 and p52, accumu

152 g fusions of the processing-related proteins fibrillarin, nucleolin, or B23 with green fluorescent pr

153 Later analyses revealed the presence of fibrillarin, nucleoporin p62, and La in BIG1 and nucleol

154 , the survival of motor neurons protein, and fibrillarin occur together in a nuclear body that is clo

155 Fibrillarin, one of the major proteins of the nucleolus,

156 RNA modifications but does not interact with fibrillarin or NOP58.

157 Co-immunostaining with antibodies to fibrillarin or p80 coilin and immunoelectron microscopy

158 cted in the coiled bodies stained for either fibrillarin or p80 coilin, a protein found only in the c

159 ions that did not affect Nop56/58 binding to fibrillarin or sRNP assembly nevertheless disrupted sRNP

160 se a model of the structural organization of fibrillarin-ORF3 protein complexes and discuss potential

161 In particular, fibrillarin (p = 0.028), centromeric protein B (p = 0.01

162 own by decreased nascent pre-rRNA synthesis, fibrillarin perinucleolar cap formation and upregulation

163 whose exceptionally stable interaction with fibrillarin plays a role in both RNP assembly and methyl

164 and polyclonal antibodies to the recombinant fibrillarin protein were generated in rabbits.

165 Prp and fibrillarin proteins are involved in RNA processing in e

166 iously identified nucleolar proteins B23 and fibrillarin, proteins with electrophoretic mobilities ch

167 f enzyme mutants identified three additional fibrillarin residues (Thr-70, Glu-88, and Asp-133) to be

168 ific upstream binding factor and the protein fibrillarin, respectively.

169 of the properties of the HgCl2-induced anti-fibrillarin response.

170 ide insight into movement of satBaMV via the fibrillarin-satBaMV-P20 RNP complex in phloem-mediated s

171 ion of mercury with the two cysteines in the fibrillarin sequence.

172 as markedly reduced but bright nucleolin and fibrillarin staining remained.

173 Notably, silencing fibrillarin suppressed satBaMV-, but not HV-, phloem-bas

174 optosis suggests that the immune response to fibrillarin that characterizes a subset of patients with

175 h was the generation of a 19-kDa fragment of fibrillarin that was not found following apoptotic or no

176 In contrast to immunization with full-length fibrillarin, the 19-kDa fragment produced anti-fibrillar

177 metric, with the C' box contacting Nop56 and fibrillarin, the C box interacting with Nop58, and the D

178 Loss of Fibrillarin, the snoRNA-guided 2'-O-methyltransferase, i

179 distinct foci of other CB markers, including fibrillarin, the survival motor neuron (SMN) protein, U2

180 itivity of the HgCl2-induced modification of fibrillarin to 2-ME, iodoacetamide, and hydrogen peroxid

181 nvolved not only in competitive binding with fibrillarin to C1QBP on 90S but also in site 2 cleavage

182 se of the cell cycle, MPP10 colocalized with fibrillarin to chromosome surfaces.

183 nserved bktRNA1 guides the methyltransferase fibrillarin to install RNA methylation of U12 small nucl

184 clear and nucleolar fractions of the protein Fibrillarin to mark and locate where it associates with

185 We also estimate the molar ratio of fibrillarin to ORF3 protein in the complexes as approxim

186 function is to target the methyltransferase fibrillarin to rRNA (for example, SNORD27 performs 2'-O-

187 5p caused the nucleolar protein Nop1p (yeast fibrillarin) to be localized to the nucleus and cytosol.

188 extract made under native conditions, where fibrillarin was not detected, indicating that a fraction

189 The archaeal fibrillarin was shorter than its eukaryotic counterpart

190 g of the eukaryotic rRNA processing protein, fibrillarin, was also upregulated sixfold in the presenc

191 cDNA for the box C/D snoRNA common protein, fibrillarin, was cloned and polyclonal antibodies to the

192 sequence derived from the in vivo substrate fibrillarin we observed that PRMT1 methylated substrates

193 of the nucleolus where neither B23, UBF, or fibrillarin were concentrated.

194 leoprotein (RNP) complex composed of P20 and fibrillarin, whereas BaMV movement proteins, capsid prot

195 ght regions contain DmNopp140 and endogenous fibrillarin, whereas the phase-dark regions contain endo

196 methionine to the target RNA is performed by fibrillarin, which by itself has no affinity for the sRN

197 ze and number, we examined the expression of fibrillarin, which did not correlate with rRNA levels.