ライフサイエンスコーパス: filopodia

1 as well as the length and stability of cell filopodia.

2 ped beads results in sustained growth of the filopodia.

3 educed the concentration of wild-type DAT in filopodia.

4 ated into a nanoscopic volume at the tips of filopodia.

5 ts, R60A and W63A, that do not accumulate in filopodia.

6 surface projections such as lamellipodia and filopodia.

7 ki adheres to surfaces using actin-dependent filopodia.

8 endogenous adhesion signaling to growth cone filopodia.

9 extend into the peripheral domain and enter filopodia.

10 nsity, F-actin content, and the formation of filopodia.

11 he density of R60A, but not that of W63A, in filopodia.

12 nd close MT apposition to actin filaments in filopodia.

13 n filaments along axon shafts giving rise to filopodia.

14 nslocation of Arp2/3 and integrin-beta along filopodia.

15 turning from focal uncaging of Ca(2+) within filopodia.

16 of filopodia elongation to produce the long filopodia.

17 actin filaments within the actin bundles of filopodia.

18 in extension direction, thus producing long filopodia.

19 e axonal periphery, including at the tips of filopodia.

20 for its striking localization to the tips of filopodia.

21 state, also increased R60A concentration in filopodia.

22 nt targeting of DAT to, and accumulation in, filopodia.

23 in filaments and the formation of actin-rich filopodia.

24 otes actin convergence to create the base of filopodia.

25 and spatiotemporal protein enrichment along filopodia.

26 and results in the formation of nonpolarized filopodia.

27 odia-like structures, and a role for SNX9 in filopodia.

28 ntent of Arp2/3 and induced the formation of filopodia.

29 known to play a role in both invadopodia and filopodia.

30 not affect the number of substrate adherent filopodia.

31 h as formins elongating these filaments into filopodia.

32 ers from the cell body and are distinct from filopodia.

33 vinculin localize as distinct patches in the filopodia.

34 (Y499) to be important for the formation of filopodia.

35 tactin to regulate density but not length of filopodia.

36 ated with extensive actin assembly and dense filopodia.

37 embrane and deterministic forces from active filopodia?

38 The role of such forces in filopodia adhesion is however insufficiently understood.

39 ion or knockdown of myosin IIA abolishes the filopodia adhesion to the beads.

40 n a formin-dependent fashion is required for filopodia adhesion.

41 dynamic MTs from extending into growth cone filopodia along actin filament bundles.

42 DCs by modeling the generation of forces by filopodia and a force balance on the cell.

43 NMIIB at the perinuclear area and tip of the filopodia and affect cell migration differentially.

44 e, Heusermann et al. show that exosomes surf filopodia and are endocytosed in a process reminiscent t

45 Lack of SDF1alpha-induced filopodia and associated abnormal cell protrusions seen

46 is) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with

47 of actin-filled membrane protrusions such as filopodia and bind to microtubules (MT), suggesting that

48 disorganized microtubules that fail to enter filopodia and co-align with actin filaments.

49 r10-dependent interactions between fine axon filopodia and developing muscles.

50 itical for formation of endothelial tip cell filopodia and EC migration.

51 tion, loss of VEGFR2 increases the number of filopodia and enhances the growth rate of new branches.

52 edge, while higher concentrations inhibited filopodia and favored lamellipodia and pre-filopodia bun

53 The basal filopodia and FN pillars are also necessary for proper s

54 eta reduces dynamic movements of growth cone filopodia and impairs presynaptic differentiation.

55 e lower in dendritic spines than in immature filopodia and increased upon expression of a nonadhesive

56 llular matrix-dependent effect, coupled with filopodia and lamellipodia formation and an enrichment o

57 kinase B (Akt) to advance the development of filopodia and lamellipodia in Chinese hamster ovary cell

58 P1 colocalized at the leading edge including filopodia and lamellipodia of migrating cells.

59 leton undergoes rapid rearrangement, forming filopodia and lamellipodia.

60 ith the morphology of their tips, which lack filopodia and lamellipodia.

61 hind during the projection and retraction of filopodia and lamellipodia.

62 le for glycolytic ATP synthesis localizes in filopodia and lammelipodia, where ATP is rapidly consume

63 ells lose luminal-abluminal polarity, extend filopodia and migrate into extravascular space where the

64 F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating ce

65 y, irradiated podocytes demonstrated loss of filopodia and remodeling of cortical actin.

66 formation and distribution of intercellular filopodia and stress fibers in follicle cells.

67 How the interplay of filopodia and synapse assembly ensures robust connectivi

68 al FERM domain regulating both the number of filopodia and their elongation velocity.

69 docytes, THSD7A expression is accentuated at filopodia and thin arborized protrusions, an expression

70 eins, this chimera trafficked to the tips of filopodia and was also able to transport individual comp

71 ell polarization, regulates the formation of filopodia, and appears to assist in the organization of

72 CD73 associated with cell-cell contacts, filopodia, and membrane zippers, indicative of involveme

73 integrin alphaL toward the cell membrane and filopodia, and secretory vesicles containing the HSP90al

74 ics of the mitochondria, plasma membrane and filopodia, and the 2D and 3D dynamics of the endoplasmic

75 Commissural filopodia appear on the floor plate to interact with the

76 Dendritic filopodia are actin-filled dynamic subcellular structure

77 Filopodia are actin-filled membrane protrusions that pla

78 Filopodia are actin-filled protrusions employed by cells

79 Filopodia are actin-rich protrusions important for sensi

80 Dendritic filopodia are actin-rich structures that are thought to

81 also contribute to filopodial assembly, and filopodia are disproportionately associated with focal a

82 igh-speed Ca(2+) imaging, we show that these filopodia are dynamic structures that contain a secretor

83 Invadopodia and filopodia are dynamic, actin-based protrusions contribut

84 Filopodia are dynamic, actin-rich structures that transi

85 Filopodia are finger-like actin-rich protrusions that ex

86 Filopodia are finger-like protrusions from the plasma me

87 e turnover and the generation of presynaptic filopodia are microglia-dependent processes.

88 sin; MF) in the initiation and elongation of filopodia are not well defined and may reflect conserved

89 Filopodia are peripheral F-actin-rich structures that en

90 Filopodia are precursors of dendritic spines and polariz

91 sequent quiescence, whereas cells with large filopodia are related to activation of early response ge

92 Filopodia are thin, fingerlike structures that contain b

93 Filopodia are ubiquitous fingerlike protrusions, spawned

94 Fascin dynamics within filopodia are under the control of formins, and in parti

95 called convergent elongation, proposes that filopodia arise from Arp2/3 complex-nucleated dendritic

96 ve observed that human neutrophils use large filopodia as cellular tentacles to sense local environme

97 ngle vesicles to the cell body by surfing on filopodia as well as filopodia grabbing and pulling moti

98 isodic Ca(2+) signals present in both motile filopodia as well as in later-stabilized synaptic bouton

99 rete foci at the leading edge, VASP promotes filopodia assembly and forms part of a poorly understood

100 le for fascin that operates independently of filopodia assembly to promote efficient cell migration a

101 and quantify spatiotemporal localization of filopodia-associated proteins during the filopodial exte

102 and separable from a role for fascin within filopodia at the cell periphery.

103 Filopodia at the growth cone periphery have long been co

104 CLAMP accumulated in lamellipodia and filopodia at the leading edge of migrating cells in asso

105 such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell

106 sequential extension-retraction of platelet filopodia attached to fibrin fibers.

107 the presence of myosin IIA filaments at the filopodia bases.

108 either sex to inform modeling of sparse and filopodia-bearing mossy fibers, finding that these circu

109 ector that automatically finds lamellipodia, filopodia, blebs and other motifs.

110 d filopodia and favored lamellipodia and pre-filopodia bundles.

111 complex inhibits the formation of dendritic filopodia but that later during development, the Arp2/3

112 we confirmed that Hdl-Myo10 does not induce filopodia, but it strongly localized to the plasma membr

113 grade flow, MT growth speed, and invasion of filopodia by MTs, orchestrating cytoskeletal dynamics to

114 nt actin structures, such as lamellipodia or filopodia, by altering the balance of these assembly fac

115 ithelial tube, extends specialized signaling filopodia called cytonemes that take up signals such as

116 ular space is by traveling along specialized filopodia called cytonemes.

117 Filopodia can be mechanosensitive and function as cell-c

118 ormally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia.

119 r spreading and higher numbers of actin-rich filopodia (cell extensions) than controls.

120 The small size and transient nature of filopodia combined with shared usage of actin regulators

121 In particular, en passant bouton and filopodia connections with CA3 interneurons peak when ad

122 O mice exhibit a 50% decrease in endothelial filopodia, demonstrating that Myo10 is required to form

123 yo16 in cortical neurons altered growth cone filopodia density and axonal branching patterns in a TRI

124 a molecular heterogeneity whereby different filopodia display markedly different responses to change

125 This filopodia-driven exploration stage may represent an esse

126 ent, we examined the behavior of growth cone filopodia during the exploration of both correct and off

127 Filopodia, dynamic membrane protrusions driven by polyme

128 We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fl

129 ites reverses 14-3-3-dependent inhibition of filopodia dynamics and cancer cell chemotaxis.

130 l effectors, resulting in down-regulation of filopodia dynamics and cancer cell migration.

131 ediated depletion of Actalpha reduces axonal filopodia dynamics and disturbs collateral branch format

132 ly, our results demonstrate that MYO3A slows filopodia dynamics and enhances filopodia lifetime in CO

133 w, actin within filopodia is responsible for filopodia dynamics by conducting simultaneous force spec

134 Using DiI-SiR, we visualized filopodia dynamics in HeLa cells over 25 min at 0.5 s te

135 NA targeting-but not translation-depolarised filopodia dynamics in motile endothelial cells and induc

136 -domain protein IRSp53 is a key regulator of filopodia dynamics that couples Rho-GTPase signaling to

137 ar tufts, abnormal cell motility and altered filopodia dynamics when live-imaged.

138 tomyosin-driven mechanism controls dendritic filopodia dynamics.

139 vidual regulatory and structural proteins as filopodia elongate and subsequently retract.

140 myosin X then initiates the second cycle of filopodia elongation to produce the long filopodia.

141 d to be capable of rescuing the formation of filopodia, establishing the minimal elements necessary f

142 During filopodia extension, we found the translocation of Arp2/

143 sin X (encoded by Myo10)-induced multi-cycle filopodia extension.

144 ein function to create a polarised domain of filopodia extension.

145 by different cross-linking proteins, such as filopodia (fascin), lamellipodia (fimbrin), and stress f

146 This is realised through the extension of filopodia filled with low intracellular pH vesicles sugg

147 Macrophage filopodia, finger-like membrane protrusions, were first

148 Surprisingly, filopodia form and elongate toward sources of Slit, a re

149 Yet the mechanism by which 14--3-3 regulates filopodia formation and dynamics and its role in cell mi

150 t the CT region contributes to DAAM-mediated filopodia formation and dynamics in primary neurons.

151 tion triggers 14-3-3 binding, which inhibits filopodia formation and dynamics, irrespective of whethe

152 on underlies diverse molecular mechanisms of filopodia formation and extension.

153 is associated with the mechanism underlying filopodia formation and extension.

154 e engineered MYO6+ offers insights into both filopodia formation and MYO6 motor function at endosomes

155 of ATP drives EC rearrangement by promoting filopodia formation and reducing intercellular adhesion.

156 eveal that the principles of MF myosin-based filopodia formation are conserved via divergent mechanis

157 carcinoma in situ, we reveal a link between filopodia formation at the cell-matrix interface, in col

158 interaction-defective mutant of Pak2 rescued filopodia formation but led to abnormal F-actin bundles.

159 iogenesis by regulating endothelial DLL4 and filopodia formation in a rat model of ischemic stroke.

160 Filopodia formation in Amoebozoa and Metazoa requires th

161 fy the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncove

162 te migration rate (PMR) and restored reduced filopodia formation in shRNA-induced DAAM2-knockdown pod

163 Defective filopodia formation is linked to pathologies such as can

164 , but the repertoire of factors required for filopodia formation is only partially understood.

165 e involved in processes such as endocytosis, filopodia formation, and cell migration.

166 raction with beta-Pix are essential for HSPC filopodia formation, cytoskeletal integrity, and homing

167 f the DdMyo7 motor is found to weakly rescue filopodia formation, further highlighting the importance

168 othelial DLL4 expression, cell motility, and filopodia formation, is expected to be a novel druggable

169 growth of formed branches without affecting filopodia formation, loss of VEGFR2 increases the number

170 PA gel required Cdc42- and formin-dependent filopodia formation, whereas adhesion to a 60-kPa Ecad-F

171 IRSp53 is an essential regulator of filopodia formation, which couples Rho-GTPase signaling

172 dent, F-actin-independent, and important for filopodia formation.

173 that subsequently recruit VASP and initiate filopodia formation.

174 icantly attenuated the myosin-X-induced long filopodia formation.

175 lateral movement, and subsequently initiated filopodia formation.

176 up formation, but displayed markedly reduced filopodia formation.

177 diated by GIPC and PI(4,5)P2 are crucial for filopodia formation.

178 erpinning precise mRNA targeting to sites of filopodia formation.

179 n of Cdc42, the small GTPase responsible for filopodia formation.

180 rtactin/Arp2/3 signaling pathway controlling filopodia formation.

181 ivity to prevent inappropriate activation of filopodia formation.

182 cues in the microenvironment, implying that filopodia foster local signal transduction, yet their sm

183 the formation and elongation of actin-based filopodia from mouse dorsal root ganglion growth cones.

184 l glia in the developing brain extend motile filopodia from their primary stalk.

185 rin-beta then became localized at the tip of filopodia, from where myosin X initiated the second exte

186 te wide biological significance, delineating filopodia function in complex systems remains challengin

187 cell body by surfing on filopodia as well as filopodia grabbing and pulling motions to reach endocyti

188 ding fixation, (ii) capturing at the tip and filopodia-guided actin anterograde flow with phagocytic

189 Filopodia have important sensory and mechanical roles in

190 ity, we collected and analyzed image data on filopodia in cultured rat hippocampal neurons.

191 as Cdc42 is required for the predominance of filopodia in early neurulation.

192 e is widely applicable, capable of detecting filopodia in four different cell types in vitro and in v

193 olecular motor that localizes to the tips of filopodia in mammalian cells.

194 The formation of filopodia in Metazoa and Amoebozoa requires the activity

195 porter (DAT) has been shown to accumulate in filopodia in neurons and non-neuronal cells.

196 led multiple mechanisms involving macrophage filopodia in particle capture and engulfment.

197 der flow revealed that platelets rather form filopodia in the thrombus shell, and are flattened with

198 We examined embryonic growth cone filopodia in vivo to directly observe their exploration

199 opy, we observe that tumor spheroids display filopodia in vivo, supporting a potential role for these

200 Myo10 is required to form normal numbers of filopodia in vivo.

201 In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an end

202 (21MT-1 and 21PT) enhances the formation of filopodia, increases cell proliferation (Ki67 expression

203 y, electron microscopy experiments on mature filopodia indeed frequently reveal actin bundles that ar

204 Here, we study filopodia induced by overexpression of myosin X, typical

205 To explore the role of filopodia-inducing Cdc42, we generated myeloid-restricte

206 Filopodia-inducing Myo10 is implicated in axon guidance

207 anges in ApoER2 abundance modulate dendritic filopodia initiation and synapse maturation.

208 nt-based structures, including lamellipodia, filopodia, invadopodia, and membrane blebs, as well as o

209 The exploratory motion of the filopodia is crucial for synaptogenesis, but the underly

210 sexes revealed that the formation of ectopic filopodia is increased in Sema2a heterozygotes.

211 investigated whether, and how, actin within filopodia is responsible for filopodia dynamics by condu

212 the dimensions of actin-rich processes like filopodia, lamellipodia, microvilli, and stereocilia req

213 filaments, comprising the structural core of filopodia, largely determine their instantaneous lengths

214 phospho-deficient cortactin mutant decreased filopodia length and density, whereas overexpression of

215 n of a 499E phospho-mimetic mutant increased filopodia length.

216 MYO3A slows filopodia dynamics and enhances filopodia lifetime in COS7 cells.

217 M10(Full)LZ localizes at the tip of filopodia like myosin-X full-length (M10(Full)).

218 pha(13) Intriguingly, PRG DH/PH also induced filopodia-like cell protrusions and activated Cdc42.

219 auses dendritic spines to adopt an elongated filopodia-like morphology.

220 a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed- and live-cell

221 Taken together, our data suggest a role of filopodia-like protrusions in mediating signaling events

222 al neurons, knockdown of mis12 increased the filopodia-like protrusions in this region.

223 Wild-type mesoderm cells have long polarized filopodia-like protrusions, which are absent in beta-Pix

224 in is nucleated within the lamina, sprouting filopodia-like spikes towards the nuclear membranes.

225 ated and have a well-defined cell soma and a filopodia-like structure.

226 ntibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro.

227 body technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopo

228 EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathogen clearance by

229 cytoskeletal rearrangements and formation of filopodia-like structures.

230 pillars are specifically established through filopodia-mediated and pulsating force-related mechanism

231 Impeding filopodia-mediated nucleation and micron-scale assembly

232 Adhesions were formed by filopodia-mediated nucleation and micron-scale assembly

233 ity protein 2 (Vangl2)-regulated increase in filopodia number and polarization.

234 (2) neurite complexity, and (3) growth cone filopodia number, in accordance with CD2AP expression le

235 zation and dynamics in both lamellipodia and filopodia of Aplysia growth cones.

236 actin filaments and dynamic microtubules in filopodia of pseudopods of invading cells under a chemot

237 ng in radial glia in vivo to eliminate glial filopodia or impair glial motility profoundly impacted s

238 Cdc42 is not critical for filopodia or phagocytic cup formation, but plays a key r

239 stablishes a role for specialized signalling filopodia, or cytonemes, in morphogen dispersion and sig

240 ident Lifeact-EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathoge

241 tic boutons as well as the generation of new filopodia originating from spine heads or boutons.

242 ely attach to the motoneuron while extending filopodia over the cell body.

243 CLAMP knock-down reduced the number of filopodia, perturbed filopodia polarity, and altered the

244 n reduced the number of filopodia, perturbed filopodia polarity, and altered the organization of acti

245 The lifetime of such filopodia positively correlates with the presence of myo

246 ay facilitate actin filament convergence for filopodia production.

247 ed by lack of compatible methods to quantify filopodia properties.

248 Alongside the FN pillars, long filopodia protrude from the basal surfaces of somite epi

249 Filopodia protrude from the leading edge of cells and pl

250 n as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical

251 TIM1-mediated ER remodeling, particularly in filopodia, regulates spatiotemporal calcium signals duri

252 factors that regulate fascin dynamics within filopodia remain poorly understood.

253 We demonstrate that filopodia repeatedly contact off-target muscles over sev

254 Motoneuron filopodia repeatedly contacted off-target muscle fibers

255 ments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all

256 Filopodia restoration was also induced by the formin-act

257 thus mediating endothelial cell motility and filopodia retraction.

258 ity induces directional growth of spine head filopodia (SHF) followed by spine relocation.

259 se and, consequently, promotes the dendritic filopodia-spines (F-S) transition.

260 soforms, Actalpha, Actbeta, and Actgamma, to filopodia stability and dynamics during this process is

261 large variety of cellular structures such as filopodia, stress fibers, cytokinetic rings, and focal a

262 en actin filament bundles and dynamic MTs in filopodia, suggesting that tau links these two cytoskele

263 Filopodia supported the phagocytic uptake of bacterial (

264 In addition, filopodia supported the uptake of zymosan (Saccharomyces

265 re at any time point only 1-2 "synaptogenic" filopodia suppress the synaptic competence of other filo

266 uclei, thicker dendrites, and more dendritic filopodia than all other groups.

267 Consistently, Ena tetramers form more filopodia than mutant dimer and trimers in Drosophila cu

268 and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry.

269 tered localization of the remaining Ezrin to filopodia that form during activation.

270 n with the emergence of actin-rich dendritic filopodia that initiate contact with presynaptic axonal

271 and robustly decreases the density of bouton filopodia that provide feedforward inhibition.

272 cells, emerges from polarized intercellular filopodia that radiate from basal stress fibers and exte

273 ther facilitate FGF22 targeting to dendritic filopodia that receive presynaptic stimulation.

274 icit local Ca(2+) signals within growth cone filopodia that regulate axon guidance through activation

275 EB1/EB3, coupling ER to microtubules, within filopodia, thereby steering growth cones.

276 They often exhibit a high density of filopodia (thin actin bundles), which complicates the un

277 ia suppress the synaptic competence of other filopodia through competition for synaptic seeding facto

278 N pillar formation is dependent on the basal filopodia through these molecules.

279 tracheal elements, which dynamically extend filopodia throughout the central nervous system (CNS) to

280 Then myosin X moves to the filopodia tip and attracts integrin-beta and Arp2/3 for

281 Here we identify precursors of this filopodia tip complex in migrating B16F1 cells: small le

282 te that assembly and disassembly dynamics of filopodia tip complexes are determined, in part, by a ne

283 equired for lamellipodin to incorporate into filopodia tip complexes, and overexpression of monomeric

284 s (30-34) in the MYO3A tail does not prevent filopodia tip localization but abolishes the ability to

285 Application of pulling forces to the filopodia tips through attached fibronectin-coated laser

286 complex that remains associated with growing filopodia tips.

287 Low levels of PFN1 caused filopodia to form exclusively at the leading edge, while

288 protein that plays a key role in stabilizing filopodia to support efficient adhesion and migration.

289 lex drives the morphological maturation from filopodia to typical spine morphology.

290 , mDia2 is known to mediate the formation of filopodia-type (immature) spines.

291 rget molecule abundance, it is revealed that filopodia typically harbor higher densities of 3' phosph

292 n networks with diverse functions, including filopodia used for cell migration and contractile rings

293 We devised a scheme to estimate the radii of filopodia using either the membrane marker or volume mar

294 , whereas localization of MTs to growth cone filopodia was facilitated by direct MT binding and inter

295 oskeleton protrusions-i.e., lamellipodia and filopodia-were reduced after treatment.

296 s, actin filaments redistribute to extending filopodia where they exhibit increased dynamics.

297 bound R60A mutant predominantly localized in filopodia, whereas free R60A molecules were evenly distr

298 must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound

299 minus (GIPC) accumulate at the tips of these filopodia, while APPL1 endosomes accumulate at the base.

300 e myosin X initiated the second extension of filopodia with a change in extension direction, thus pro