ライフサイエンスコーパス: fission yeast

1 ffic to promote septum-driven cytokinesis in fission yeast.

2 ecting cell diameter in the rod-shaped model fission yeast.

3 cting RAS GTPase to nutrient availability in fission yeast.

4 dels in matched GI datasets from baker's and fission yeast.

5 and constriction of the contractile ring in fission yeast.

6 sure proper splicing of certain pre-mRNAs in fission yeast.

7 epigenetic inheritance of heterochromatin in fission yeast.

8 acterize the transcriptome in the meiosis of fission yeast.

9 RNAs), suggesting that dsRNAs form widely in fission yeast.

10 ion and influences cell cycle progression in fission yeast.

11 e isoform level in the sexual development of fission yeast.

12 ic tubule elongation and vesicle scission in fission yeast.

13 physical mechanism for typical conditions in fission yeast.

14 subunit and, thereby, controls exocytosis in fission yeast.

15 function of PS in the rod-shaped, polarized fission yeast.

16 ner similar to what has been demonstrated in fission yeast.

17 are coordinated to maintain lipid balance in fission yeast.

18 3 act in parallel to inhibit TOR function in fission yeast.

19 pt4 in controlling elongation in budding and fission yeast.

20 nesis functions of Cdc14/Flp1 in budding and fission yeast.

21 recent experiments on histone methylation in fission yeast.

22 ce a robust anti-stress cellular response in fission yeast.

23 tromeres cluster at the spindle pole body in fission yeast.

24 dscapes of the distantly related budding and fission yeast.

25 nd is a key determinant of cohesive sites in fission yeast.

26 stinct action of its GEFs, Gef1 and Scd1, in fission yeast.

27 reminiscent of natural polarity patterns in fission yeast.

28 novel protein, Rng10, during cytokinesis in fission yeast.

29 ent of heterochromatin at pericentromeres in fission yeast.

30 ed CDC mutants in the very distantly related fission yeast.

31 is catalyzed by the cohesin loader, Mis4 in fission yeast.

32 activity against Gram-positive bacteria and fission yeast.

33 mitochondrial and nuclear DNA of budding and fission yeast.

34 rapid genomic gene editing and regulation in fission yeast.

35 e II (RNAPII), and are mutually dependent in fission yeast.

36 p coordinate cytokinetic furrow formation in fission yeast.

37 tion speed and depth are two-fold greater in fission yeast.

38 RdDM, reflecting an analogous interaction in fission yeast.

39 ssembling genomes and centromeres of related fission yeasts.

40 se transcript landscapes between budding and fission yeasts.

41 which are absent or divergent in budding or fission yeasts.

42 on in both mammalian and yeast cells, and in fission yeast a single mitotic cyclin can drive the cell

43 In fission yeast, a JmjC domain protein, Epe1, promotes tra

44 t during quiescence, the unicellular haploid fission yeast accumulates mutations as a linear function

45 eviously discovered that competition between fission yeast actin binding proteins (ABPs) for binding

46 The fission yeast actin cytoskeleton is an ideal, simplified

47 By using mutants of the fission yeast actin severing protein Adf1, we observed t

48 Here, we develop a strategy for loading the fission yeast Ago1 with a single-stranded sRNA guide, wh

49 Whereas in fission yeast all kinetochores relaxed to a similar leng

50 MP as a highly sensitive calcium reporter in fission yeast, allowing us to capture calcium transients

51 ivity and resistance conferring mutations in fission yeast, along with biochemical assays with recomb

52 Fission yeast AMPK catalytic subunit Ssp2 is phosphoryla

53 A similar response can be observed in fission yeast and bacteria.

54 and regulatory basis of energy metabolism in fission yeast and beyond, and it pinpoints weaknesses of

55 We have tested Pomegranate on fission yeast and demonstrate its ability to 3D segment

56 fic manner in Schizosaccharomyces pombe Both fission yeast and human Sde2 are translated as inactive

57 Using in vivo live imaging in fission yeast and in vitro MT dynamics assays, we show t

58 In radially symmetric cells, such as fission yeast and many bacteria, this 2D segmentation ca

59 two zones of actin filaments is specific for fission yeast and not essential for CME.

60 omoter choices for potential applications in fission yeast and other organisms.

61 age analysis portfolio already available for fission yeast and other radially symmetric cell types.

62 Our results elucidate the Loz1 regulon in fission yeast and provide new insight into how eukaryoti

63 s with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells.

64 led previously undetected origin clusters in fission yeast and shows that in human cells replication

65 r target recognition in the TORC2 complex in fission yeast and the mTORC2 complex in mammals.

66 ed up to the length of entire chromosomes in fission yeast and up to 12 Mb fragments in human cells.

67 studies of Cdc42 polarization in budding and fission yeasts and demonstrate that models describing sy

68 pe in mammals, spindle pole body dynamics in fission yeast, and surveillance of defective nuclear por

69 i-mediated post-transcriptional silencing in fission yeast, and unveil an important role for post-tra

70 he RNA-binding characteristics we observe in fission yeast are likely to apply to related proteins in

71 anches formed from purified muscle actin and fission yeast Arp2/3 complex and observed debranching ev

72 Using fission yeast as a model, we report here that DBAN delay

73 oles of GR in cellular stress response using fission yeast as a model.We surprisingly discovered mark

74 Here, we show that fission yeast Aurora B localizes at telomeres during mei

75 ypes of data to form a comprehensive view of fission yeast biology.

76 Fission yeast Brc1 has emerged as a key factor in promot

77 rtholog) are required for both activities in fission yeast but whether they are genetically separable

78 points contributes to kinetochore capture in fission yeast, but the relative contributions of dynamic

79 daptor Nro1 regulate the hypoxic response in fission yeast by controlling activity of the sterol regu

80 ess, we have carried out a genetic screen in fission yeast by random mutation of the genome, looking

81 Remarkably, using passive cross-linkers, fission yeast can assemble a bipolar spindle in the abse

82 Expression of fission yeast CDC25 (Spcdc25) in tobacco results in smal

83 We report here that Rga6 is another fission yeast Cdc42 GAP which shares some functions with

84 only GAP described as negative regulator of fission yeast Cdc42.

85 Using fission yeast cell cycle as an example, we uncovered tha

86 g the stability, speed and robustness of the fission yeast cell cycle oscillations.

87 equentiality, as observed in the budding and fission yeast cell- cycle.

88 In further investigations of the budding and fission yeast cell-cycle, we identify two generic dynami

89 opy (FPALM), we probed this question in live fission yeast cells at unprecedented resolution.

90 s required for oxidative stress responses in fission yeast cells by promoting transcription initiatio

91 Here, we show that fission yeast cells carrying a mutation in the DNA-bindi

92 We show that fission yeast cells deficient in ER-PM contacts exhibit

93 Rod-shaped fission yeast cells divide at a threshold size partly du

94 ing of mRNA quantities is apparent in single fission yeast cells during a normal cell cycle.

95 We study cell polarization when fission yeast cells exit starvation.

96 examine the transcriptomes of >2,000 single fission yeast cells exposed to various environmental con

97 inuous replication of hundreds of individual fission yeast cells for over seventy-five generations.

98 Rod-shaped fission yeast cells grow in a highly polarized manner, a

99 phatases, promotes the symmetric division of fission yeast cells through spatial control of cytokines

100 erresolution localization microscopy of live fission yeast cells to improve the spatial resolution to

101 roscopy, and mathematical modeling in single fission yeast cells to uncover the precise molecular mec

102 of the popular green-to-red PCFP mEos3.2 in fission yeast cells under a wide range of imaging condit

103 In fission yeast cells, Cyclin B(Cdc13) scales with size, a

104 okinesis nodes and contractile rings in live fission yeast cells.

105 chromatin, we affinity-selected solubilized fission yeast CENP-A(Cnp1) chromatin.

106 g genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for terminat

107 hizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus.

108 onary divergence of this response within the fission yeast clade.

109 single misshaped cells, and even across the fission yeasts clade.

110 similar structures appear to be conserved in fission yeast, computational modeling and analysis of hi

111 e, we use efficient conditional depletion of fission yeast condensin to determine its contribution to

112 The fission yeast contractile ring has been proposed to asse

113 Using in vitro reconstitution of fission yeast contractile ring precursor nodes containin

114 gulation of nonmuscle tropomyosins, which in fission yeast controls actin filament stability and divi

115 Fission yeast Ctp1 and its budding yeast (Sae2) and huma

116 ivered by myosin-V on linear actin cables in fission yeast cytokinesis.

117 In fission yeast, cytokinesis involves the type II myosins

118 icient contractile ring assembly in vivo.The fission yeast cytokinetic ring assembles by Search-Captu

119 The developmental asymmetry of fission yeast daughter cells derives from inheriting 'ol

120 a GFP-based protein reporter and screened a fission yeast deletion collection using flow cytometry.

121 By screening the fission yeast deletion library, we found that heterochro

122 A large region of fission yeast DNA inserted into a mouse chromosome was p

123 oscopy indicate that the heterochromatinised fission yeast DNA is organised into smaller chromatin lo

124 l to analyze these results, we conclude that fission yeast does not age and that cellular aging and r

125 The related SHREC complex in fission yeast drives transcriptional gene silencing in h

126 actin nucleation plays an essential role in fission yeast endocytosis.

127 cerbated when combined with mutations in the fission yeast Ensa homologue, Igo1.

128 In the absence of mga2, fission yeast exhibited growth defects under both normox

129 In fission yeast, Exo1 is the primary resection nuclease, w

130 hat during transcriptional activation of the fission yeast fbp1 gene, binding of Rst2 (a critical C2H

131 ed lncRNAs (mlonRNAs) in the promoter of the fission yeast fbp1 gene, whose transcription is massivel

132 In fission yeast, force balance is restored when both kines

133 ith this, we show that R-loops formed at the fission yeast gene sum3 do not form detectable R-loop ob

134 ntragenic antisense transcription of ~10% of fission yeast genes, with each perturbation affecting la

135 d facultative heterochromatin islands in the fission yeast genome and found that RNA elimination mach

136 ve heterochromatin are maintained within the fission yeast genome through self-reinforcing mechanisms

137 matin at endogenous and ectopic sites in the fission yeast genome.

138 Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosom

139 In fission yeast, glucose starvation triggers lncRNA transc

140 complex are more sensitive to debranching by fission yeast GMF (glia maturation factor) than branches

141 During closed mitosis in fission yeast, growing microtubules push onto the nuclea

142 nt epimutants resistant to caffeine arise in fission yeast grown with threshold levels of caffeine.

143 k we determined the interaction of Clr4, the fission yeast H3K9 methyltransferase, with nucleosomes u

144 where a putative histone demethylase Epe1 in fission yeast, has a non-enzymatic function that opposes

145 In the last few decades, experiments on fission yeast have revealed different molecular players

146 Cell cycle mutants in the budding and fission yeasts have played critical roles in working out

147 Using the fission yeast, here, we track G0-associated chromatin an

148 ith each other in vitro, and at least in the fission yeast, heterologous Oxs1 and Pap1-homologues can

149 In fission yeast, histone H3 lysine 9 (H3K9) is methylated

150 Erh1, the fission yeast homolog of Enhancer of rudimentary, is imp

151 Here, we identify Fft3, a fission yeast homolog of the mammalian SMARCAD1 SNF2 chr

152 We find that the fission yeast homologues of Tristetraprolin/TTP and Pumi

153 We phenotype gene-deletion strains of fission yeast in 59,350 individual fitness assays in 70

154 Here we investigate the role of fission yeast inner nuclear membrane proteins in determi

155 Here we show that in fission yeast, introducing the K9M mutation into one of

156 t early divergence from a common ancestor in fission yeast involved important changes in the mechanis

157 is type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repe

158 Mer2, identified so far only in budding and fission yeasts, is in fact evolutionarily conserved from

159 s effective in predicting the DNA content of fission yeast, it is likely to have a broad application

160 for the bidirectional motility mechanism of fission yeast kinesin-5 and provide insight into the fun

161 olution structure of the motor domain of the fission yeast kinesin-5 Cut7 bound to fission yeast micr

162 s, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromo

163 Here, we describe a multiplexed fission yeast lifespan micro-dissector (multFYLM) and an

164 carried out a large-scale genetic screen in fission yeast looking for mutants with enhanced sensitiv

165 ivity of human Aurora A but also for that of fission yeast MAPK-activated kinase (Srk1) and PKA (Pka1

166 heterochromatin invasion by manipulating the fission yeast mating type locus boundary using a single-

167 In fission yeast, Mcp5 is the anchor protein of dynein and

168 In fission yeast, medial division is controlled through neg

169 In fission yeast, meiosis-specific transcripts are selectiv

170 In contrast, distinct pathways activate fission yeast Mga2 and Sre1.

171 of the fission yeast kinesin-5 Cut7 bound to fission yeast microtubules and explored the topology of

172 indle, we developed a computational model of fission-yeast mitosis.

173 ocking region, the hydrophobic patch, on the fission yeast mitotic cyclin Cdc13 as a potential mechan

174 he recent identification of Spo13 orthologs, fission yeast Moa1 and mouse MEIKIN, suggests that kinas

175 chromosome territories are organized in the fission yeast model organism.

176 riven cytokinesis stems extensively from the fission yeast model system.

177 Here, we show that fission yeast Mso1 is also a Sec1-binding protein and Ms

178 Fission yeast Mso1 shares homology with budding yeast Ms

179 , we discovered that the myosin I protein in fission yeast, Myo1, which is required for organization

180 ysical model of kinetochore capture in small fission-yeast nuclei using hybrid Brownian dynamics/kine

181 of nucleation-promoting factors (NPFs) as in fission yeast or a single ring of NPFs as in budding yea

182 suggests a mechanistic conservation between fission yeast PAF1 repressing AGO1/small interfering RNA

183 hat the greatwall-endosulfine (Ppk18-Igo1 in fission yeast) pathway couples the nutritional environme

184 Here we address this issue via analysis of fission yeast peptidyl-prolyl isomerase Pin1.

185 Fission yeast phosphate acquisition genes pho1, pho84, a

186 The fission yeast phosphate homeostasis (PHO) regulon compri

187 Fission yeast phosphate homeostasis genes are repressed

188 A new study in fission yeasts promotes the notion that transient polari

189 omyosin ring is essential for cytokinesis in fission yeast, proper furrow formation also requires sep

190 Moreover, the LIM domain region from the fission yeast protein paxillin like 1 (Pxl1) also locali

191 emonstrate the importance of uncharacterized fission yeast proteins Mso1 and Sec1 in membrane traffic

192 As a model for cellular energy regulation, fission yeast provides an attractive and complementary s

193 This analysis of H3-G34R mutant fission yeast provides mechanistic insight into how G34R

194 parts list of genes important for meiosis in fission yeast, providing a valuable resource to advance

195 n-containing region from mammalian zyxin and fission yeast Pxl1 binds to mechanically stressed F-acti

196 Fission yeast Rai1 also has HDH activity although it doe

197 res of mammalian DXO with 3'-FADP or CoA and fission yeast Rai1 with 3'-FADP provide elegant insight

198 Here, we identify new fission yeast regulatory lncRNAs that are targeted, at t

199 ibonucleotide maps from both the budding and fission yeast reveal conservation of these processes.

200 y, a superresolution microscopy study of the fission yeast ring revealed that myosins and formins tha

201 e a coarse-grained mathematical model of the fission yeast ring to explore essential consequences of

202 ere we present quantitative evidence that in fission yeast, ring tension originates from barbed-end a

203 g was highlighted by experiments on isolated fission yeast rings, where sections of ring became unanc

204 We examine the function of Swi1 and Swi3, fission yeast's primary FPC components, to elucidate how

205 The fission yeast scaffold molecule Sid4 anchors the septum

206 y interfering with scaling in the rod-shaped fission yeast Schizosaccharomyces japonicus that relies

207 probed these fundamental questions using the fission yeast Schizosaccharomyces japonicus, which break

208 Here, using the fission yeast Schizosaccharomyces pombe (a classical mod

209 Here we use the fission yeast Schizosaccharomyces pombe as a model to in

210 plored cytokinetic calcium transients in the fission yeast Schizosaccharomyces pombe by adopting GCaM

211 For example, rod-shaped fission yeast Schizosaccharomyces pombe cells, which div

212 quest to understand the morphogenesis of the fission yeast Schizosaccharomyces pombe drove us to inve

213 ean laboratories in the 1940s and 1950s, the fission yeast Schizosaccharomyces pombe has grown to bec

214 Studies in fission yeast Schizosaccharomyces pombe have provided th

215 Here, using Clr4, the fission yeast Schizosaccharomyces pombe homologue of mam

216 e oxygen-responsive lipid homeostasis in the fission yeast Schizosaccharomyces pombe in a manner anal

217 We recently showed that drug tolerance in fission yeast Schizosaccharomyces pombe is controlled by

218 re we present evidence that cell size in the fission yeast Schizosaccharomyces pombe is regulated by

219 The two PKC orthologs Pck1 and Pck2 in the fission yeast Schizosaccharomyces pombe operate in a red

220 Fission yeast Schizosaccharomyces pombe P and M cells, w

221 ory element-binding proteins (SREBPs) in the fission yeast Schizosaccharomyces pombe regulate lipid h

222 Here, we utilize the fission yeast Schizosaccharomyces pombe to investigate h

223 sue we performed ribosome profiling with the fission yeast Schizosaccharomyces pombe under conditions

224 In the fission yeast Schizosaccharomyces pombe, active Cdc42 an

225 In the fission yeast Schizosaccharomyces pombe, dynamic cytopla

226 In the fission yeast Schizosaccharomyces pombe, H3K9me heteroch

227 In the fission yeast Schizosaccharomyces pombe, heterochromatin

228 In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores sh

229 In the rod-shaped fission yeast Schizosaccharomyces pombe, symmetric divis

230 In the fission yeast Schizosaccharomyces pombe, the CaMKK-like

231 In the fission yeast Schizosaccharomyces pombe, the formin For3

232 In the fission yeast Schizosaccharomyces pombe, the multi-BRCT

233 In the fission yeast Schizosaccharomyces pombe, the protein kin

234 In the fission yeast Schizosaccharomyces pombe, the SREBP-2 hom

235 In the fission yeast Schizosaccharomyces pombe, TORC1 is essent

236 Here, in fission yeast Schizosaccharomyces pombe, we successfully

237 study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe.

238 sterol flow between PM and endosomes in the fission yeast Schizosaccharomyces pombe.

239 A translation during histidine starvation in fission yeast Schizosaccharomyces pombe.

240 egulators have come from in vivo analysis in fission yeast Schizosaccharomyces pombe.

241 f meiotic drive genes on chromosome 3 of the fission yeasts Schizosaccharomyces kambucha and S. pombe

242 NE management strategies between the related fission yeasts Schizosaccharomyces pombe and Schizosacch

243 ress this fundamental question using related fission yeasts Schizosaccharomyces pombe and Schizosacch

244 he most dramatic shift in gene expression in fission yeast (Schizosaccharomyces pombe), and this resp

245 In fission yeast (Schizosaccharomyces pombe), genetic scree

246 In fission yeast (Schizosaccharomyces pombe), mitochondria

247 Here, motivated by work in fission yeast (Schizosaccharomyces pombe), we generated

248 inc-requiring Pho8 alkaline phosphatase from fission yeast (Schizosaccharomyces pombe).

249 atalog of genes important for meiosis in the fission yeast, Schizosaccharomyces pombe Our genome-wide

250 In fission yeast, Schizosaccharomyces pombe, interactions b

251 To investigate these features for the fission yeast, Schizosaccharomyces pombe, we developed a

252 haracterized example of sizer behavior is in fission yeast, Schizosaccharomyces pombe, which enters m

253 he cleavage furrow during cytokinesis of the fission yeast, Schizosaccharomyces pombe.

254 Similar to the human shelterin, fission yeast shelterin is composed of telomeric sequenc

255 ypotheses, yielding additional evidence that fission yeast siRNA-Argonaute silencing complexes are re

256 inding mutant shows a reduced association of fission yeast SMC5/6 with chromatin.

257 The fission yeast specific chaperone Rng3 was thus not requi

258 We demonstrate that, similarly in fission yeast, spindle length and spindle dynamics adjus

259 Proteolytic release of fission yeast SREBPs from the membrane in response to lo

260 genic suppressors of a temperature-sensitive fission yeast strain mutated in the exocyst subunit Sec3

261 Here we show that the fission yeast stress-activated protein kinase Sty1, a ho

262 Therefore, knowledge from the budding and fission yeast systems illuminates highly conserved molec

263 In fission yeast, Tel1(ATM)/Rad3(ATR)-mediated phosphorylat

264 p1, an uncharacterized NAP family protein in fission yeast that antagonizes CENP-A loading at both ce

265 sight into the role of H3-G34R, we generated fission yeast that express only the mutant histone H3.

266 started a program of searches for mutants in fission yeast that revealed a range of phenotypes inform

267 assembly in Schizosaccharomyces japonicus, a fission yeast that undergoes partial mitotic NE breakdow

268 We show in fission yeast that, at low Cdc20 concentrations, Cdc20(M

269 Here we demonstrate, in both budding and fission yeast, that kinetochores and KNL1(Spc105/Spc7) c

270 In fission yeast, the correct ordering of CDK substrate pho

271 In fission yeast, the highly conserved ortholog of human ER

272 In fission yeast, the inverted repeats IR-L and IR-R functi

273 In fission yeast, the septation initiation network (SIN) en

274 In fission yeast, the ste11 gene encodes the master regulat

275 In fission yeast, the two HP1 proteins Chp2 and Swi6 assume

276 ied single-molecule speckle tracking in live fission yeast to directly measure molecular turnover wit

277 thyltransferase PRMT3 that is conserved from fission yeast to humans.

278 Here, we use fission yeast to investigate how phosphatase activity pa

279 and FPALM superresolution microscopy of live fission yeast to investigate the structures and assembly

280 From fission yeast to mammals, heterochromatin assembly at DN

281 In fission yeast, TORC2 is dispensable for proliferation un

282 Here, we report the crystal structure of the fission yeast Tpz1(475-508)-Poz1-Rap1(467-496) complex t

283 onclude that like the larger animal embryos, fission yeast triggers calcium transients that may play

284 Here, we provide evidence that the fission yeast tropomyosin, Cdc8, is regulated by phospho

285 which the motor activity and function of the fission yeast type one myosin, Myo1, is modulated by TOR

286 mic bundling is important for cytokinesis in fission yeast, we created the less dynamic bundling muta

287 In the isogamous organism fission yeast, we employed high-resolution fluorescence

288 Here using fission yeast, we identify Fun30(Fft3) as a chromatin re

289 ere, using a non-essential minichromosome in fission yeast, we identify roles for the HR factors Rqh1

290 Using live-cell imaging of fission yeast, we provide evidence that Dip1 is a single

291 Using replication fork barriers in fission yeast, we report that relocation of arrested for

292 To characterize R-loops in fission yeast, we used the S9.6 antibody-based DRIPc-seq

293 l1p, the main eisosome BAR-domain protein in fission yeast, we visualized whole eisosomes and, after

294 espondingly, upon onset of closed mitosis in fission yeast, wherein interphase microtubules assemble

295 omponents and the PCM by taking advantage of fission yeast, which has a centriole-free, PCM-containin

296 cytokinesis arrest in the erg11-1 mutant of fission yeast, which has a partial defect in the biosynt

297 Here, we show that fission yeast whole-cell poly(A)(+) RNA-protein crosslin

298 rding the human gene homologs in budding and fission yeast, worm, fly, fish, mouse, and rat on a sing

299 Pho7 is the Schizosaccharomyces pombe fission yeast Zn(2)Cys(6) transcriptional factor that dr

300 antified the localization pattern of Mcp5 in fission yeast zygotes and show by perturbation of phosph