ライフサイエンスコーパス: flap endonuclease

1 nces relative to replication origins and the flap endonuclease.

2 clamp loader), family B DNA polymerase, and flap endonuclease.

3 lies at the base of the archway in phage T5 flap endonuclease.

4 a supplement or backup for the Rad27/Fen1 5' flap endonuclease.

5 port that the RAG1/ RAG2 recombinase is a 3' flap endonuclease.

6 ve 5'-3' exonuclease and secondarily as a 5'-flap endonuclease.

7 members such as FEN1, GEN1 is a monomeric 5'-flap endonuclease.

8 of the EXO1 gene, which encodes an enigmatic flap endonuclease.

9 EN1 is a member of the XPG/Rad2 family of 5'-flap endonucleases.

10 ncodes T4 RNase H, a relative of a family of flap endonucleases.

11 cific endonuclease activity similar to known flap endonucleases.

12 acts with DNA polymerase beta (Pol-beta) and flap endonuclease 1 (Fen-1) and blocks Pol-beta-directed

13 replication proteins, including the enzymes flap endonuclease 1 (FEN-1) and DNA ligase I that comple

14 ociation of condensin I with the BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/eps

15 The interaction between flap endonuclease 1 (FEN-1) and proliferation cell nucle

16 approach has been demonstrated that utilizes flap endonuclease 1 (FEN-1) fused to the Fok1 endonuclea

17 helicase-endonuclease that participates with flap endonuclease 1 (FEN-1) in Okazaki fragment processi

18 Human flap endonuclease 1 (FEN-1) is a member of the structure

19 PCNA binds to flap endonuclease 1 (FEN-1), a structure-specific endonu

20 ases is with the structure-specific nuclease Flap Endonuclease 1 (FEN-1), an enzyme that is implicate

21 Here we show that NEIL1 interacts with flap endonuclease 1 (FEN-1), an essential component of t

22 (RFC) complex, DNA polymerase delta (POLd), flap endonuclease 1 (FEN-1), and DNA ligase 1 (LIG1).

23 lly bound DNA polymerase beta (Pol beta) and flap endonuclease 1 (FEN-1).

24 a protein domain of WRN which interacts with flap endonuclease 1 (FEN-1).

25 stically, we showed that MLH1 interacts with Flap endonuclease 1 (FEN1) and competes to process the R

26 ivities of DNA polymerase delta (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (Lig1).

27 he enzymes DNA polymerase delta (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (LigI) that

28 Flap endonuclease 1 (FEN1) and Dna2 are responsible for

29 Flap endonuclease 1 (FEN1) and Dna2 endonuclease/helicas

30 Flap endonuclease 1 (Fen1) and exonuclease 1 (Exo1) have

31 Human flap endonuclease 1 (FEN1) and related structure-specifi

32 This flap structure was cleaved by flap endonuclease 1 (Fen1) and the resultant nick was li

33 onuclease 1 (APE1) physically interacts with flap endonuclease 1 (FEN1) and with proliferating cell n

34 DNA polymerase beta (pol beta) and flap endonuclease 1 (FEN1) are key players in pol beta-m

35 The Dna2 helicase/nuclease and then flap endonuclease 1 (FEN1) are proposed to act sequentia

36 Dna2 nuclease/helicase and flap endonuclease 1 (FEN1) are proposed to cleave the fl

37 This stimulated flap endonuclease 1 (FEN1) cleavage of TNRs engaged in a

38 Flap endonuclease 1 (FEN1) cleaved the displaced downstr

39 uted with purified enzymes demonstrated that Flap endonuclease 1 (FEN1) efficiently excises a displac

40 AMP-dRP group through its lyase activity and flap endonuclease 1 (FEN1) excises the 5'-AMP-dRP group

41 The structure-specific endonuclease flap endonuclease 1 (FEN1) has also been suggested to be

42 Flap endonuclease 1 (FEN1) is a central component of Oka

43 Flap endonuclease 1 (Fen1) is a highly conserved structu

44 Flap endonuclease 1 (FEN1) is a long-standing target of

45 Flap endonuclease 1 (FEN1) is a protein that plays a rol

46 Flap endonuclease 1 (FEN1) is a structure-specific nucle

47 Flap endonuclease 1 (FEN1) is a structure-specific nucle

48 Flap endonuclease 1 (FEN1) is a structure-specific nucle

49 Flap endonuclease 1 (FEN1) is a structure-specific nucle

50 5'-Flap endonuclease 1 (Fen1) is an important enzyme involv

51 We identify that the flap endonuclease 1 (FEN1) is one of the interacting pro

52 that the DNA replication and repair protein Flap endonuclease 1 (FEN1) is required for replication o

53 DNA replication and repair enzyme Flap Endonuclease 1 (FEN1) is vital for genome integrity

54 Flap endonuclease 1 (FEN1) participates in removal of RN

55 Flap endonuclease 1 (FEN1) phosphorylation is proposed t

56 Flap endonuclease 1 (FEN1) plays a crucial role in both

57 Flap Endonuclease 1 (FEN1) plays important roles both in

58 Here, we characterized a flap endonuclease 1 (FEN1) plus hairpin DNA probe (hpDNA

59 nuclease 1 (FAN1), exonuclease 1 (EXO1), and flap endonuclease 1 (FEN1) process a substrate reminisce

60 Flap endonuclease 1 (FEN1) processes Okazaki fragments i

61 Flap endonuclease 1 (FEN1) proteins, which are present i

62 nent in the maturation of Okazaki fragments, flap endonuclease 1 (FEN1) removes the 5'-flap and maint

63 In one model for this process, the flap endonuclease 1 (FEN1) removes the iRNA.

64 e proteome of FA-induced DPCs and found that flap endonuclease 1 (FEN1) resolves FA-induced DPCs.

65 s in concert with alternate flap cleavage by flap endonuclease 1 (FEN1) to mediate CAG repeat expansi

66 cates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to process the Okazaki fragme

67 s derived from Xenopus laevis, we found that flap endonuclease 1 (FEN1) was a factor responsible for

68 Recently, Flap endonuclease 1 (FEN1) was shown to contribute to te

69 Most flaps are cleaved by flap endonuclease 1 (FEN1) while short, and the remainin

70 Here, we demonstrate that flap endonuclease 1 (FEN1), a canonical lagging strand D

71 Here we show RAD27/FEN1, which encodes flap endonuclease 1 (FEN1), a structure-specific nucleas

72 Human flap endonuclease 1 (FEN1), an essential DNA replication

73 Polymorphisms and somatic mutations in Flap Endonuclease 1 (FEN1), an essential enzyme involved

74 se 1 (APE1), DNA polymerase beta (pol beta), flap endonuclease 1 (FEN1), and DNA ligase I (LigI).

75 n genes involved in DNA replication, such as flap endonuclease 1 (FEN1), can cause single-stranded DN

76 p21 disrupts PCNA-directed stimulation of flap endonuclease 1 (FEN1), DNA ligase I, and DNA polyme

77 AP endonuclease, replication factor C, PCNA, flap endonuclease 1 (FEN1), DNA polymerase delta, and DN

78 Flap endonuclease 1 (Fen1), has an endonuclease activity

79 In most cases, the flap was removed by flap endonuclease 1 (FEN1), in a reaction required to re

80 Flap endonuclease 1 (FEN1), involved in the joining of O

81 d cancer avoidance of the structure-specific flap endonuclease 1 (FEN1), its cellular compartmentaliz

82 , which is removed by the structure-specific flap endonuclease 1 (FEN1), leaving a nick for ligation.

83 is via long-patch BER (LP-BER) dependent on flap endonuclease 1 (FEN1), not previously known to be p

84 and handoff of DNA-editing enzymes, such as flap endonuclease 1 (FEN1), with sliding clamps are key

85 Flap endonuclease 1 (FEN1)-dependent long-patch repair h

86 brogated two of three nuclease activities of flap endonuclease 1 (FEN1).

87 age of the flap by Dna2 nuclease followed by flap endonuclease 1 (FEN1).

88 he primer into a flap that is cleaved off by flap endonuclease 1 (FEN1).

89 Okazaki fragments generated by Pol delta and Flap endonuclease 1 (FEN1).

90 Human flap endonuclease 1 (h-FEN1) mutations have dramatic eff

91 long flap (20 nucleotides) by M. acetivorans flap endonuclease 1 (MacFEN1).

92 e with a 5'- and 3'-flap that was cleaved by flap endonuclease 1 and a 3'-5' endonuclease Mus81/Eme1,

93 o profoundly altered PCNA's interaction with Flap endonuclease 1 and DNA Ligase 1, DNA metabolism enz

94 tion factor C complex, DNA polymerase delta, flap endonuclease 1 and DNA ligase 1.

95 these processes, acting in conjunction with flap endonuclease 1 and replication protein A in DNA lag

96 ses, apurinic/aprymidinic endonuclease 1 and flap endonuclease 1 and several other factors involved i

97 Treatment of extracts with flap endonuclease 1 antiserum significantly reduced MHEJ

98 Instead, flap endonuclease 1 must recognize and bind to the inter

99 We further demonstrate that hDNA2 and flap endonuclease 1 synergistically process intermediate

100 evolution methodology that uses the nuclease flap endonuclease 1 to achieve the efficient discovery o

101 ssed by DNA polymerase beta and processed by flap endonuclease 1 with different efficiency.

102 beled the eukaryotic DNA replication protein flap endonuclease 1 with mKikGR and added it to replicat

103 of the related RAD2 family nucleases, FEN1 (Flap endonuclease 1) and EXO1 (exonuclease 1), on substr

104 protein complex (LIG1, pol B, aprataxin, and flap endonuclease 1) can coordinate to prevent the forma

105 In most cases, FEN1 (flap endonuclease 1) is able to efficiently cleave short

106 Until cleaved by FEN1 (flap endonuclease 1), such flaps can initiate homologous

107 provide strand displacement synthesis, human flap endonuclease 1, and human DNA ligase I.

108 Finally, we found that aprataxin and flap endonuclease 1, as compensatory DNA-end processing

109 This resembles the mechanism of flap endonuclease 1, consistent with cooperation of thes

110 isomer of RECQL5, RECQL5beta, with the human flap endonuclease 1, FEN1, which plays a critical role i

111 ase/endonuclease, called RAD2 homologue 1 or flap endonuclease 1, has a unique cleavage activity, dep

112 Coordinating with flap endonuclease 1, the APE1 3'-5' exonuclease activity

113 a long flap that was efficiently cleaved by flap endonuclease 1, thereby leading to repeat deletion.

114 Flap endonuclease 1, which preferentially cleaves unanne

115 g is not channeled to the subsequent enzyme, flap endonuclease 1.

116 A and provides binding sites for polymerase, flap endonuclease-1 (FEN-1) and ligase during DNA replic

117 Flap EndoNuclease-1 (FEN-1) and the processivity factor

118 replication protein A (RPA), which inhibits flap endonuclease-1 (FEN-1) but stimulates Dna2 nuclease

119 y transfer (FRET) analyses show that WRN and Flap Endonuclease-1 (FEN-1) form a complex in vivo that

120 Flap endonuclease-1 (FEN-1) is a critical enzyme for DNA

121 Human flap endonuclease-1 (FEN-1) is a member of the structure

122 Human flap endonuclease-1 (FEN-1) is a member of the structure

123 Flap endonuclease-1 (FEN-1) is a multifunctional and str

124 Human flap endonuclease-1 (FEN-1) is a structure-specific endo

125 Mammalian flap endonuclease-1 (FEN-1) is a structure-specific meta

126 Flap endonuclease-1 (FEN-1) is a structure-specific nucl

127 termediates in DNA replication and repair by flap endonuclease-1 (FEN-1) is essential for mammalian g

128 a Caenorhabditis elegans homologue of human flap endonuclease-1 (FEN-1) that is normally involved in

129 Human flap endonuclease-1 (Fen-1) was examined for its ability

130 Flap endonuclease-1 (FEN-1), a 43-kDa protein, is a stru

131 rimidinic endonuclease, DNA polymerase beta, flap endonuclease-1 (FEN-1), and PARP-1.

132 olog-1 (RTH-1) class nuclease, also known as flap endonuclease-1 (FEN-1).

133 Flap endonuclease-1 (FEN1) belongs to the Rad2 family of

134 Flap endonuclease-1 (FEN1) is a multifunctional, structu

135 Flap endonuclease-1 (FEN1) is proposed to participate in

136 The prototypical 5'-nuclease, flap endonuclease-1 (FEN1), catalyzes the essential remo

137 cture- and strand-specific phosphodiesterase flap endonuclease-1 (FEN1), the prototypical 5'-nuclease

138 Interaction between human flap endonuclease-1 (hFEN-1) and proliferating cell nucl

139 Human flap endonuclease-1 (hFEN-1) is highly homologous to hum

140 Human flap endonuclease-1 (hFEN1) catalyzes the essential remo

141 ion of Okazaki fragments escapes cleavage by flap endonuclease-1 and anneals to a complementary ectop

142 sion repair proteins DNA polymerase beta and flap endonuclease-1 by 4-6-fold.

143 pt annealing at the ectopic site and promote flap endonuclease-1 cleavage.

144 substrate recognition and specificity of the flap endonuclease-1 enzymes.

145 Bacteriophage T4 RNase H, a flap endonuclease-1 family nuclease, removes RNA primers

146 This is the first structure of a flap endonuclease-1 family protein with its complete bra

147 cates a back-up function of exonuclease 1 to flap endonuclease-1 in RNA primer removal during lagging

148 Flap endonuclease-1 or FEN-1 is a structure-specific and

149 The role of human FEN1 (flap endonuclease-1), an RTH1 (RAD two homolog-1) class

150 Addition of Flap endonuclease-1, a nuclease known to remove 5' overh

151 Finally, a combination of HIV-1 RT, Flap endonuclease-1, and DNA ligase is capable of quanti

152 ivity of HEX1-N2 is similar to that of human flap endonuclease-1, both in terms of turnover efficienc

153 lymerase-1 (PARP-1) and the BER participants flap endonuclease-1, DNA polymerase beta, and apurinic/a

154 es lacking genes encoding the orthologues of flap endonuclease-1, PCNA, and MutS.

155 or recombination that may overlap with human flap endonuclease-1.

156 ctional enzyme composed of an N-terminal DNA flap endonuclease/5' exonuclease domain (FEN/EXO) and a

157 action of the WRN gene product with human 5' flap endonuclease/5'-3' exonuclease (FEN-1), a DNA struc

158 on of the WRN gene product with the human 5' flap endonuclease/5'-3' exonuclease (FEN-1), a DNA struc

159 raction of the BLM protein with the human 5'-flap endonuclease/5'-3' exonuclease (FEN-1), a genome st

160 e 1 (hExo1) possesses both 5'exonuclease and flap endonuclease activities and plays a role in DNA rep

161 ell understood, its 5' to 3'-exonuclease and flap endonuclease activities may cleave intermediates th

162 rocessive 5'-3' exonuclease and secondary 5'-flap endonuclease activities participate in various DNA

163 Both the 5' to 3' exonuclease and flap endonuclease activities require a divalent metal co

164 OEX1 has 5'-3'-exonuclease and flap endonuclease activities, with a high affinity for R

165 It has both 5' nuclease and flap endonuclease activities.

166 hPCNA-mediated stimulation of both exo- and flap endonuclease activities.

167 activity but significant single- and double-flap endonuclease activities.

168 Mutation of the Arg-70 significantly reduced flap endonuclease activity and eliminated exonuclease ac

169 ystal structures of related enzymes with the flap endonuclease activity and propose that there are tw

170 rad27-G240Dp displayed a significant double-flap endonuclease activity but was devoid of exonuclease

171 mutant Rad27 and FEN-1 enzymes with partial flap endonuclease activity but without nick-specific exo

172 The flap endonuclease activity is less active relative to it

173 Furthermore, although the flap endonuclease activity of FEN1 E359K was unaffected,

174 ctions between Fen1 and hChlR1 stimulate the flap endonuclease activity of Fen1.

175 The flap endonuclease activity of HEX1-N2 is similar to that

176 Flap endonuclease activity of the wild type and mutant e

177 nant human CtIP and find that it exhibits 5' flap endonuclease activity on branched DNA structures, i

178 The native exonuclease also had strong flap endonuclease activity similar to that observed with

179 T4 RNase H also has a flap endonuclease activity that cuts preferentially on e

180 Flap endonuclease activity was demonstrated in oocyte nu

181 Escherichia coli, the Xenopus enzymes showed flap endonuclease activity, a unique feature of this cla

182 Each enzyme has a flap endonuclease activity, cutting at or near the junct

183 the bacterial transposase Tn10 contains a 3' flap endonuclease activity, suggesting a mechanistic par

184 lease activity and showed only a weak single-flap endonuclease activity.

185 s been no biochemical evidence demonstrating flap endonuclease activity.

186 l acidic residue results in complete loss of flap endonuclease activity.

187 uplex as the 5'-tail inhibits gene 6 protein flap endonuclease activity.

188 ' exonuclease activity of Rad2p and not its "flap endonuclease" activity and is absolutely dependent

189 esidue is conserved in the structures of all flap endonucleases analysed to date.

190 esindicates that GST-Rad2p possesses both 5'-flap endonuclease and 5'-->3' double-stranded DNA exo-nu

191 suggest that PCNA mediates the entry of the flap endonuclease and DNA ligase I into the process of O

192 rs are removed from Okazaki fragments by the flap endonuclease and DNA ligase I joins nascent fragmen

193 SAV6 is an FEN1 homologue that shows double-flap endonuclease and gap-dependent endonuclease activit

194 Next, DNA repair activities of DNA ligase, flap endonuclease and RNase H2 were monitored.

195 Contacts between the 5' flap endonuclease and the sugar-phosphate backbone of th

196 t is processed by a 5'-3' exonuclease (or 5'-Flap endonuclease) and that NucS-promoted MMR is indepen

197 PCNA, or reverse transcriptase), a nuclease (flap endonuclease), and a ligase (ligase I, III, or IV a

198 eat tracts, mutations of RAD27, encoding the flap endonuclease, and CDC9, encoding DNA ligase I, incr

199 lycosylase, AP endonuclease, DNA polymerase, flap endonuclease, and DNA ligase activities.

200 We also propose that XPF, a 3'-flap endonuclease, and LIG3, a DNA ligase, are involved

201 The enzyme has 5'-3' exonuclease, flap endonuclease, and weak RNaseH activity in vitro, bu

202 ermore, BRCA2-deficient cells require the 5' flap endonuclease but not the 5'-3' exonuclease activity

203 Human LP-BER was driven by the Fen1 flap endonuclease, but it did not include 5' excision.

204 and RAD1, encoding part of the Rad1/Rad10 3'-flap endonuclease, caused synthetic growth defects in ye

205 Deletion of RAD27, which encodes a flap endonuclease, causes inviability in mre11 strains.

206 pair functions through its 5'-3'-exonuclease/flap-endonuclease domain.

207 The 5'-flap endonuclease Fen-1 is essential for Okazaki fragmen

208 EN) activities but retains almost all of its flap endonuclease (FEN) activity, we show severe defects

209 The flap endonuclease (FEN) of the hyperthermophilic archaeo

210 ndonuclease activity that is provided by the flap endonuclease (FEN-1) in the nucleus, resulting in m

211 Flap endonuclease (FEN-1) removes 5' overhanging flaps i

212 e repeats form structures that inhibit human flap endonuclease (FEN-1).

213 ermediates became substrates for cleavage by flap endonucleases (FEN-1 proteins).

214 In the presence of the flap endonuclease FEN1 (Rad27), the complex carried out

215 2 heterodimer, bound to a single copy of the flap endonuclease FEN1 at 2.9 A resolution.

216 The conserved, structure-specific flap endonuclease FEN1 cleaves 5' DNA flaps that arise d

217 nd ubiquitylation, cooperate to target human flap endonuclease FEN1 to degradation by the proteasome

218 flap pathway, Pol delta coordinates with the flap endonuclease FEN1 to degrade initiator RNA, whereas

219 ised by RNase H2, and further excised by the flap endonuclease FEN1 with strand displacement synthesi

220 dinated actions of DNA polymerase delta, the flap endonuclease FEN1, and DNA ligase I.

221 of DNA polymerase delta (Pol delta) and the FLAP endonuclease FEN1.

222 ynthetically lethal with mutations in the 5'-flap endonuclease FEN1/Rad27 in Saccharomyces cerevisiae

223 e that the exonuclease activity of mammalian flap endonuclease (FEN1) excises Pol alpha replication e

224 to cut at the base of single-stranded flaps, flap endonuclease (FEN1) is now recognized as a central

225 Flap endonuclease (Fen1) is required for DNA replication

226 idence indicates that null mutants of the 5'-flap endonuclease (FEN1) result in an expansion of repet

227 rate to a double flap that can bind Dna2 and flap endonuclease (FEN1) simultaneously.

228 combined activities of polymerase B (PolB), flap endonuclease (Fen1), and DNA ligase are required to

229 Flap endonuclease (FEN1), essential for DNA replication

230 Pol beta activity is enhanced by flap endonuclease (FEN1), which cleaves the resulting fl

231 ught to be removed by RNase HI and the 5'-3' flap endonuclease (FEN1).

232 The flap endonuclease, FEN1, is an evolutionarily conserved

233 The flap endonuclease, FEN1, plays a critical role in DNA re

234 Flap endonucleases (FENs) catalyse the exonucleolytic hy

235 During replication and repair flap endonucleases (FENs) catalyze endonucleolytic and e

236 Flap endonucleases (FENs) isolated from archaea are show

237 The flap endonucleases (FENs) participate in a wide range of

238 Flap endonucleases (FENs), essential for DNA replication

239 ed member of a novel subfamily of ubiquitous flap endonucleases (FENs), which possess only one of the

240 The Invader technology uses a Flap Endonuclease for allele discrimination and a univer

241 cloned three open reading frames encoding a flap endonuclease from Archaeglobus fulgidus, Methanococ

242 mensional model of the structure-specific 5' flap endonuclease from Pyrococcus furiosus in its comple

243 phosphate diester hydrolysis catalysed by a flap endonuclease has been studied.

244 Rad2, a FEN-1 flap endonuclease homolog, is involved in processing Oka

245 role of one of the DNA replication factors, flap endonuclease I (FEN1), in regulating telomerase act

246 a 1- or 10-nucleotide flap DNA substrate by Flap Endonuclease I.

247 show the importance of the C terminus of the flap endonuclease in DNA replication and repair and, by

248 Crenarchaeal XPF, a 3'-flap endonuclease, is also stimulated by PCNA in vitro.

249 We recovered the flap endonuclease mutation rad27-K325* with a stop codon

250 ol delta depended on RAD1, which encodes the flap endonuclease needed to cleave MMEJ intermediates be

251 The flap endonucleases, or 5' nucleases, are involved in DNA

252 removed by strand displacement synthesis and flap endonuclease processing via a long patch repair mec

253 In addition, S. pombe mutant for the flap endonuclease rad2 gene, whose precise function in D

254 mediated by exonuclease 1 (Exo1) and by the flap endonuclease Rad27 (human FEN1) coupled with DNA po

255 haromyces cerevisiae, the MMR system and the flap endonuclease Rad27 act in overlapping pathways that

256 teraction of DNA polymerase delta and the 5'-flap endonuclease Rad27/Fen1 with the PCNA sliding clamp

257 Mutants lacking the flap endonuclease Rad27p showed little change in the exp

258 Flap endonucleases remove flap structures generated duri

259 Genetic analysis revealed that the flap endonucleases Slx4 and Sae2 represent new pathways

260 ase 1 (Exo1) is a 5'-->3' exonuclease and 5'-flap endonuclease that plays a critical role in multiple

261 A replication and repair, is the major human flap endonuclease that recognizes and cleaves flap DNA s

262 To further refine the model, 5' flap endonuclease variants with alanine point substituti

263 Dna2, a flap endonuclease with 5'-3' helicase activity, is invol

264 tion-coupled ICL repair, we show that the 3' flap endonuclease XPF-ERCC1 cooperates with SLX4/FANCP t