ライフサイエンスコーパス: flexible linker

1 nto "head" and "core" domains connected by a flexible "linker".

2 inding domain, a MAPK substrate domain and a flexible linker.

3 rate-binding module, which is attached via a flexible linker.

4 motif) domains, connected by a 20-amino acid flexible linker.

5 d thioester domain moieties linked by a long flexible linker.

6 ng paraquat-based macrocycle by folding of a flexible linker.

7 omains (NTD and CTD) that are connected by a flexible linker.

8 ys to be glycosylated, which is present in a flexible linker.

9 rdomain motions in CaM-4Ca(2+), enabled by a flexible linker.

10 RfaH consists of two domains connected by a flexible linker.

11 er of two amphipathic helices connected by a flexible linker.

12 4 (Ffh) and a signal peptide connected via a flexible linker.

13 and a membrane domain joined by a 33-residue flexible linker.

14 onding to the B and C domains connected by a flexible linker.

15 n which the two subunits were connected by a flexible linker.

16 IgG heavy chain or light chain using a long flexible linker.

17 e polymerase domain through a structured but flexible linker.

18 and the C-terminal domain (CTD), joined by a flexible linker.

19 ggesting a role as a relatively nonspecific, flexible linker.

20 n connected to the variable light chain by a flexible linker.

21 and POU(S), connected by a relatively short flexible linker.

22 get form a bipartite duplex with an unpaired flexible linker.

23 o equivalent domains connected by a somewhat flexible linker.

24 nged in a predetermined order connected by a flexible linker.

25 reveals two globular domains connected by a flexible linker.

26 ed to the evolving library through an inert, flexible linker.

27 C-terminal QUA2 region by means of a highly flexible linker.

28 composed of IL-7 and HGFbeta connected by a flexible linker.

29 main and the Homeodomain connected by a long flexible linker.

30 and is separated into two short helices by a flexible linker.

31 Helix 1 is joined to helix 2 by a flexible linker.

32 -loop structure connected to rhodopsin via a flexible linker.

33 inal DNA-binding domain separated by a long, flexible linker.

34 posed of two globular domains connected by a flexible linker.

35 prises two functional domains separated by a flexible linker.

36 nal domain connected to the core domain by a flexible linker.

37 interact but are connected by an apparently flexible linker.

38 ded into well-defined domains separated by a flexible linker.

39 n), connected by an approximately 60-residue flexible linker.

40 consists of two domains connected by a long, flexible linker.

41 main connected to the other motifs through a flexible linker.

42 o a DNA-binding carboxy-terminal module by a flexible linker.

43 -terminal DNA-binding domain, separated by a flexible linker.

44 -terminal domain and its relationship to the flexible linker.

45 from the substrate through CUL5 to the RBX2 flexible linker.

46 catalytic and a lectin domain connected by a flexible linker.

47 e interconnected by the longest and the most flexible linker.

48 hat is connected to the FtsZ core by a long, flexible linker.

49 ossibility of coupling two binders through a flexible linker.

50 f two Ru(phen)2dppz(2+) moieties joined by a flexible linker.

51 ase domain and a helicase domain linked by a flexible linker.

52 and conserved modules, connected by a short flexible linker.

53 eract with each other and are connected by a flexible linker.

54 ain of cTnC, cNTnC and cCTnC) connected by a flexible linker.

55 nct N- and C-terminal domains separated by a flexible linker.

56 we created concatenated IP3R linked by short flexible linkers.

57 a hybrid DNA gel containing stiff tubes and flexible linkers.

58 nits, arranged in a linear chain joined with flexible linkers.

59 ch formed by portions of two helices and two flexible linkers.

60 gement of eight structured domains linked by flexible linkers.

61 three pleckstrin homology domains coupled by flexible linkers.

62 del of rigid filaments connected by multiple flexible linkers.

63 a(2)-microglobulin, and H chain connected by flexible linkers.

64 rivatives covalently linked via (Gly(3)-Ser) flexible linkers.

65 domains comprised of alpha-helices joined by flexible linkers.

66 rget-specific antibodies via nanometer-scale flexible linkers.

67 ate when they are tethered to a complex with flexible linkers.

68 , comprises a series of domains connected by flexible linkers.

69 d, but these domains are connected in Gag by flexible linkers.

70 ein containing discrete domains connected by flexible linkers.

71 , short basepaired regions connected by more flexible linkers.

72 horus ligands attached to the metals and the flexible linkers.

73 the contributions of the unfolded state and flexible linkers.

74 our understanding of the unfolded state and flexible linkers.

75 protein-protein interactions constrained by flexible linkers.

76 croglobulin, and heavy chain are attached by flexible linkers.

77 lengths, which have been proposed to act as flexible linkers.

78 120 and the ectodomain of gp41 are joined by flexible linkers.

79 for protein, DNA, or RNA and also including flexible linkers.

80 ng independently of each other at the end of flexible linkers.

81 mon chemical linkage groups through a set of flexible linkers.

82 ed to those of two related cages with longer flexible linkers.

83 re of a repeat protein scaffold and avoiding flexible linkers.

84 cted to the N-terminal four-helix bundle via flexible linkers.

85 of independently folded domains connected by flexible linkers.

86 s C- or N-terminus via three- or ten-residue flexible linkers.

87 location of its head domains, facilitated by flexible linkers.

88 are connected to a tetrameric stalk through flexible linkers.

89 omposed of two discrete domains connected by flexible linkers.

90 All domains are connected by flexible linkers.

91 ms adopt many conformations enabled by three flexible linkers.

92 Here we analyze three usages of flexible linkers: 1), intramolecular binding of proline-

93 dimeric neomycin-neomycin conjugate 3 with a flexible linker, 2,2'-(ethylenedioxy)bis(ethylamine), ha

94 ions: an EF-hand domain (PC2-EF, 720-797), a flexible linker (798-827), and an oligomeric coiled coil

95 our domains: the globular N-terminal core, a flexible linker, 8-9 conserved residues implicated in in

96 etal center is attached to the surface via a flexible linker (a propyl group), which allows the activ

97 ed of two globular domains linked by a short flexible linker: a catalytic domain and a ricin-like lec

98 I(Mtl)) comprises three domains connected by flexible linkers: a transmembrane domain (C) and two cyt

99 A long (14-residue), flexible linker afforded much faster electron transfer b

100 /-0.1) ns, respectively), are connected by a flexible linker (Ala147-Pro149), and do not give rise to

101 alently attached to the enzyme complex via a flexible linker, allowing the direct transfer of substra

102 This flexible linker allows the catalytic domain to move betw

103 ations of the chromophores, attached to long flexible linkers, also play an important role.

104 thesized that cTnT-Delta160E repositions the flexible linker, altering weak actomyosin electrostatic

105 porating a C-terminal extension comprising a flexible linker and 10-19 of the N-terminal residues of

106 The RPN13 PRU domain is followed by a flexible linker and a C-terminal deubiquitylase adaptor

107 s (RRMs): a tandem RRM1 and 2, followed by a flexible linker and a C-terminal RRM3.

108 .2.2]octane core was achieved by attaching a flexible linker and a potential second pharmacophore via

109 on motif forming a rigid unit, followed by a flexible linker and an alpha-helical core domain.

110 he two RBDs are separated by a 12 amino acid flexible linker and do not interact with one another in

111 , another synaptic SNARE subunit, contains a flexible linker and exhibits an atypical conjoined Q(bc)

112 h is loosely connected to the MO domain by a flexible linker and is far away from the catalytic site,

113 In contrast, C2 is attached to VC1 by a flexible linker and is fully independent.

114 uggesting that the domains are tethered by a flexible linker and lack a fixed orientation relative to

115 itionally, a short peptide that contains the flexible linker and RBD of Mre11 acts as an inhibitor of

116 r p53 binding to MdmX in the presence of the flexible linker and the intramolecular binding motif by

117 alyses to gain insights into the role of the flexible linker and the residues critical for the domain

118 n systems, even for systems that have highly flexible linkers and exhibit no domain-domain or domain-

119 These and other examples suggest that flexible linkers and sequence motifs tethered to them, l

120 A (2-methylphenoxy)acetic acid headgroup, a flexible linker, and a five-membered heteroaromatic cent

121 ssrA-tag binding and dimerization domain, a flexible linker, and a short peptide module that docks w

122 was then fused to the engineered stalk with flexible linkers, and a Factor Xa cleavage site was inse

123 tension probes displaying different ligands, flexible linkers, and fluorescent reporters, enabling th

124 us metal binding domains (MBDs) connected by flexible linkers, and these MBDs all can receive Cu(+) f

125 in the S. cerevisiae proteome indicated that flexible linkers are a common theme for PCNA-interacting

126 ded conformation in solution so that shorter flexible linkers are needed for larger peptide cores to

127 Flexible linkers are often found to tether binding seque

128 or interactions between domains connected by flexible linkers are predicted to be in the millimolar r

129 requires the use of a bulky chelator with a flexible linker attached to a Cys residue to bind Tb(3+)

130 mains, with the PLAT domains floating on the flexible linkers away from the main body of the dimer.

131 intramolecular binding motif by assuming the flexible linker behaves as a wormlike chain.

132 usion conformation, we have inserted a long, flexible linker between gp120 and gp41 in our stable gp1

133 n assays show that Siah-1 interacts with the flexible linker between SIP N and CS domains.

134 Because of the presence of a flexible linker between the OSCP and the biotinylation s

135 vage in an intervening domain creates a long flexible linker between the thioester domain and the mac

136 D, C-terminal NADH, and FAD domains, and the flexible linker between them is essential for optimal in

137 are likely to function independently with a flexible linker between them.

138 range of electrostatic interactions with the flexible linkers between coiled coils.

139 highlighted the critical role played by the flexible linkers between homologous domains.

140 nformational search space, combining it with flexible linkers between ligand binding repeats to inter

141 rminal, central, and C-terminal domains with flexible linkers between neighboring domains.

142 ophene skeleton, with a two-carbon (rigid or flexible) linker between the 5-position of the thiophene

143 d soluble gp140 construct, BG505.SOSIP, with flexible linkers can result in molecules that do not req

144 p tetramers, suggesting bifurcation into two flexible linkers clamped by inter-subunit covalent links

145 represents the hinge region positioned as a flexible linker connecting structurally isolated Fc and

146 s, consistent with a structural model with a flexible linker connecting the distal C-terminal domain

147 A flexible linker connecting the N-terminal domain (NTD) a

148 and dehydrogenase domains, consistent with a flexible linker connecting the two domains.

149 between the two dsRBDs that differs from the flexible linker connecting the two dsRBDs in the antivir

150 A flexible linker connects the central and the catalytic d

151 ar dynamics simulations on several GalNAc-T2 flexible linker constructs show altered remote prior gly

152 P8, attached to the body of the subunit by a flexible linker containing approximately 10 residues, is

153 ly preserved regions, mediated and guided by flexible linkers, defines the site of interaction with t

154 enetic fusion of two di-alpha-globins with a flexible linker demonstrated a decreased stability relat

155 Disordered flexible linkers (DFLs) are abundant and functionally im

156 ycosylation preferences, confirming that the flexible linker dictates the rotation of the lectin doma

157 In general, the presence of flexible linkers disrupted binding affinity, possibly du

158 richia coli, the CTT contains a structurally flexible linker domain and a small metal-binding domain

159 on distance is determined by the length of a flexible linker domain that connects the two dsRBDs.

160 al DNA-binding modules rotate freely about a flexible linker, enabling them to contact several arrang

161 iated disorder-to-order transition of SNAP25 flexible linker facilitates its interaction with syntaxi

162 The addition of a 20-residue flexible linker (FL20) between the gp120 and gp41 ectodo

163 The CTT consists of a flexible linker (FLD) and a small metal-binding domain (

164 at the fourth bridgehead position provides a flexible linker for attachment of effector molecules suc

165 Pc derivative 24, with a highly flexible linker group, and pc derivative 28, with a dend

166 The sequence of this flexible linker has an identity of 51% based on multiple

167 ludes two of the three CD4 sites even when a flexible linker has relieved the covalent constraint bet

168 r proteins with multiple domains tethered by flexible linkers have variable global architectures.

169 ed J-domain and a CSL-domain connected via a flexible linker-helix.

170 hRPA70 fragments containing the NTD and the flexible linker (hRPA70(1-168)).

171 nected to a ssDNA-binding domain (SSB1) by a flexible linker (hRPA70(1-326)).

172 s unique to the full-length conjugate with a flexible linker, implying that the structural context of

173 omain as well as in the participation of the flexible linker in membrane binding are proposed.

174 aves as a force transducer, rigid spacer, or flexible linker in proteins.

175 el-homology region, which are connected by a flexible linker in the heterodimer, communicate in such

176 t with success in quantifying the effects of flexible linkers in binding affinity enhancement and in

177 and enzymatic activity, and also the role of flexible linkers in mediating ubiquitin transfer and rea

178 Our results highlight the importance of flexible linkers in regulating multidomain chromatin bin

179 The unfolded state and flexible linkers in the folded structure play essential

180 orous metal-organic framework materials with flexible linkers in which the pore openings, as characte

181 the growing barbed end, we propose that the flexible linker influences the lifetime of this transloc

182 unoglobulin domains C1 and C2 connected by a flexible linker, interacts with the S2 segment of myosin

183 tein secretion activity, suggesting that the flexible linker is essential for the rod function.

184 conformational polymorphism facilitated by a flexible linker is intrinsic to NS1, and this may be the

185 , conferred by an interdomain glutamate-rich flexible linker, is critical for regulation of sigma(S)

186 We found that the flexible linker joining the two halves of the FH2 dimer

187 A flexible linker joins the recognition and lysis domains,

188 A flexible linker joins these to the dimeric nuclease doma

189 hat, together with the top of the stalk, the flexible linker keeps H heads on a leash long enough to

190 ptor and with binding sites held together by flexible linkers, lead to nearly quantitative clustering

191 amino-terminal coiled coil of Vps22 and the flexible linker leading to the ubiquitin-binding NZF dom

192 UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM an

193 A flexible linker (Lnk2) composed of 26 amino acids connec

194 conformational heterogeneity and relies on a flexible linker located between the catalytic and the le

195 We propose that the flexible linkers may allow PKR to productively dimerize

196 Flexible linkers may play three types of roles: (a) link

197 signed antiviral nanoparticles with long and flexible linkers mimicking HSPG, allowing for effective

198 in the monomeric protein are separated by a flexible linker, must communicate with each other at som

199 rd formed by two enhanced GFPs (eGFPs) and a flexible linker of 15 aminoacids (eGFP15eGFP) with this

200 ir degree of magnetic alignment, even with a flexible linker of 18 residues, exhibiting D(a) values o

201 The flexible linker of OmpR enables the second monomer to bi

202 is linked to the N terminus of another by a flexible linker of ten glycine/serine residues, has been

203 xible hinge region of the antibody or in the flexible linker of the peptide-Fc fusion proteins.

204 In this study, we inserted flexible linkers of 100 or 200 amino acid residues betwe

205 known atomic structure possibly connected by flexible linkers of known sequence, are assembled accord

206 ctors have been designed with either a short flexible linker or a set of rigid helical linkers.

207 alphaABD was joined with cadherin through a flexible linker or if it was replaced with an actin-bind

208 CG2 proteins joined either with or without a flexible linker peptide were expressed at the plasma mem

209 Flexible linker peptides connecting rigid protein domain

210 quantitative understanding of the role that flexible linkers play in intramolecular binding and prov

211 se gels; however, fusion of CaM to MBP via a flexible linker provides sufficient restriction of trans

212 ttached to ss(2)-microglobulin (ss(2)M) by a flexible linker (Qa-1 determinant modifier (Qdm)-ss(2)M)

213 e mainly to a considerably longer N-terminal flexible linker region (144 aa longer than in human).

214 Here, we investigated the importance of a flexible linker region (amino acids 93-111, referred to

215 ) and an effector domain (ED) separated by a flexible linker region (LR).

216 A flexible linker region between three fragments allows an

217 idues 118-194 contain the Hc alpha-helix and flexible linker region controlling transition of syntaxi

218 hese results indicate that the length of the flexible linker region is critical for interaction of ub

219 Vif contains a flexible linker region located at the hinge of the VCBC

220 Addition of an alanine residue to the flexible linker region of the Rieske protein lowers the

221 Additionally, a Syntaxin1A mutant lacking a flexible linker region that allows NRD movement abolishe

222 ed to hRpn1 and hRpn13, as expected from the flexible linker region that connects the two UIMs; nonet

223 in composed of an N-terminal domain (NTD), a flexible linker region, and a C-terminal domain (CTD).

224 x 2 from Chaetomium thermophilum (ctPRC2), a flexible linker region, but not the H3K27M cancer mutant

225 ransmembrane helix (helix-1) connected via a flexible linker region, including a Glu-Tyr-Arg (EYR) mo

226 This flexible linker region, which is unique for each K(2P)-c

227 in and a dimerization domain, connected by a flexible linker region.

228 egulator consists of two domains joined by a flexible linker region.

229 DNA binding domain, which are separated by a flexible linker region.

230 is connected to a transmembrane anchor by a flexible linker region.

231 ody movement of SRP domains connected by the flexible linker region.

232 contain four structured domains connected by flexible linker regions.

233 This demonstrates that the flexible linker regulates functional properties as well

234 A flexible linker (residues 197-209) connects the domains,

235 roles is the fact that unfolded proteins and flexible linkers sample many different conformations.

236 -coil domain to the protein through a short, flexible linker sequence, with the approximate length of

237 nected to the globular catalytic domain by a flexible linker sequence.

238 cular details of their interactions with the flexible linker sequences and enabled construction of fu

239 Flexible linker sequences, rather than interactions betw

240 Moreover, replacing the core aromatic with a flexible linker significantly improved selectivity.

241 omprised of three domains partitioned by two flexible linkers termed interdomain regions (IDRs).

242 HMG box domains, A and B, linked by a short flexible linker that allows the two domains to behave in

243 RRE function, provided they are joined by a flexible linker that allows the two domains to face each

244 stems and shown that DnaG interacts with the flexible linker that connects the N- and C-terminal doma

245 r(81) in the central sequence functions as a flexible linker that connects two structurally independe

246 a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monom

247 A branched flexible linker that incorporates a fluorescent dansyl m

248 he p53 binding domain of MdmX by a conserved flexible linker that is 85 residues long.

249 show that the two domains are connected by a flexible linker that is short enough to keep the binding

250 tween the minimal DNA-binding domain and the flexible linker that joins the DNA-binding domain to the

251 the additional deletion of the highly acidic flexible linker that lies between RBD and the main body

252 th the two domains connected by a 24-residue flexible linker that passes through the substrate-bindin

253 ned N- and C-terminal modules separated by a flexible linker that swivels by approximately 30 degrees

254 thalene diimide (NDI) units are connected by flexible linkers that alternate between the minor and ma

255 suggests that multivalent ligands containing flexible linkers that are longer than the spacing betwee

256 Additional domains, connected by flexible linkers that pass through the central opening o

257 mprising both CBM2bs covalently joined via a flexible linker, there was an approximate 18-20-fold inc

258 f BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adj

259 nal Inr binding domain (IBD) connected via a flexible linker to a C-terminal domain (C domain).

260 In R221239, there is a flexible linker to a furan ring that permits interaction

261 nding human antibody domains fused through a flexible linker to an engineered one-domain soluble huma

262 nsists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain.

263 omain having to undergo a large shift on its flexible linker to bind tRNA(Tyr) or the intron RNA on e

264 ubsequently conjugated these aptamers with a flexible linker to construct ultra-high-affinity bidenta

265 properties are covalently linked with a long flexible linker to create a bivalent ligand with signifi

266 ses conserved acidic segments separated by a flexible linker to grasp Rpn3 and Rpn7.

267 C terminus of NpSRII is connected by a short flexible linker to NpHtrII is active in phototaxis signa

268 ligand conformational change that allows the flexible linker to pass through the DNA duplex.

269 (ZA; the active ingredient in Relenza) via a flexible linker to poly-l-glutamine (PGN) enhances the a

270 they could be linked with a nanometer-scale flexible linker to produce bivalent ligands with improve

271 An oligoglycol was used as a flexible linker to produce macrocyclic polyether-linked

272 A glycine residue above W583 might act as flexible linker to rearrange the tryptophan gate.

273 ary of 9- and 10-mer peptides tethered via a flexible linker to the N terminus of beta2 microglobulin

274 sensory rhodopsin I (SRI) is connected by a flexible linker to the N-terminus of its transducer (Htr

275 onis sensory rhodopsin II (SRII), fused by a flexible linker to the two transmembrane helices of its

276 of blue fluorescent protein fused through a flexible linker to TRPC.

277 s and the unfolding of a protein attached by flexible linkers to an atomic force microscope.

278 solvent-compatible, must be tethered by the flexible linkers to the N-terminal domain for the produc

279 ng two FokI nuclease domains, connected by a flexible linker, to a ZFP with an N-terminal mitochondri

280 length up to propyl (C3), with longer, more flexible linkers (up to C5) providing no additional bene

281 assembly, as the insertion of a 5-amino-acid flexible linker upstream of the zipper domain leads to b

282 Long flexible linkers used to attach the oligonucleotides to

283 awS1 at multiple positions, and in situ, its flexible linker was removed, yielding fully mature heter

284 A flexible linker was required to maintain antinociceptive

285 ity of bivalent ligands with nanometer-scale flexible linkers, we constructed aptamer-based bivalent

286 nonraft transmembrane sequence containing a flexible linker were expressed in a cell line derived fr

287 enosine or 2'-deoxyadenosine units joined by flexible linkers were studied by femtosecond transient a

288 ins two alpha-helical regions connected by a flexible linker, whereas the N-terminus remains unstruct

289 e N- and C-terminal domains are joined via a flexible linker which enables them to function independe

290 , comprises two domains separated by a short flexible linker, which allows CaM to assume a wide range

291 ding domains (POUS and POUHD) connected by a flexible linker, which interact with DNA in a bipartite

292 ein with seven globular domains connected by flexible linkers, which enable substantial interdomain m

293 n (CTD) comprising four alpha-helices, and a flexible linker with a 310-helix connecting the two stru

294 Replacement of the acidic residues in the flexible linker with alanine elevates the Mre11 activity

295 ity density for the end-to-end vector of the flexible linker with L residues to have a distance d(0).

296 be effective in binding, and that the use of flexible linkers with lengths somewhat greater than the

297 re prepared, each labeled via nanometer size flexible linkers with short complementary oligonucleotid

298 Antigen peptide is conjugated using flexible linkers with short complementary oligonucleotid

299 own to interact with RNA polymerase, and two flexible linkers within the C-terminal domains may assis

300 B-34, but also reveal roles for the two long flexible linkers within the protein fragment, a result t