ライフサイエンスコーパス: floor plate

1 ns with precise trajectories parallel to the floor plate.

2 misguidance of commissural axons towards the floor plate.

3 g and turn anteriorly only after exiting the floor plate.

4 EphB receptors are expressed at the ventral floor plate.

5 cretion, is conditionally knocked out in the floor plate.

6 s in a Shh dosage-dependent reduction of the floor plate.

7 sonic hedgehog (Shh) from the notochord and floor plate.

8 he floor plate or extend directly across the floor plate.

9 ith, and regulated by, Shh expression in the floor plate.

10 iminates primary motoneurons, but not medial floor plate.

11 trajectory on the contralateral side of the floor plate.

12 ntributes to notochord, prechordal plate and floor plate.

13 d in the longitudinal plane, parallel to the floor plate.

14 hog (Shh), secreted by the notochord and the floor plate.

15 for commissural axons in the vicinity of the floor plate.

16 y1 expression is absent from the prospective floor plate.

17 ormally attracts motor neurons closer to the floor plate.

18 one, most prominently in the alar region and floor plate.

19 of inductive signals from the notochord and floor plate.

20 notochord and have a reduced, discontinuous floor plate.

21 mmissural growth cones upon contact with the floor plate.

22 ment of ventral neuroectoderm, including the floor plate.

23 Hedgehog, secreted by the notochord and the floor plate.

24 atic clusters, the motor nuclei, next to the floor plate.

25 ll as agenesis of DA neurons in the midbrain floor plate.

26 o3.2 transcripts in axons that encounter the floor plate.

27 row along the opposite direction towards the floor plate.

28 ibuting to the axon guidance function of the floor plate.

29 midbrain dopaminergic (mDA) neurons and the floor plate.

30 ion in repelling neuron cell bodies from the floor plate.

31 and E3 epithelia originate from the anterior floor plate.

32 s using guidance molecules released from the floor plate.

33 xial mesoderm that lies directly beneath the floor plate.

34 ntrolateral funiculus, and never reenter the floor plate.

35 of the chick embryo to the notochord and the floor plate.

36 le navigating their intermediate target, the floor plate.

37 fically inactivated Shh in the notochord and floor plate.

38 of the neural tube in cells that make up the floor plate.

39 nt5a, and Wnt5b) expressed in and around the floor plate.

40 itors for mDNs reside at the ventral midline floor plate, a region that also serves as a source of in

41 alon-diencephalon border and adjacent to the floor plate, a source of the secreted signaling molecule

42 mmissural neurons grow toward and across the floor plate, a specialized structure located at the vent

43 line of the neural tube, particularly in the floor plate, a structure with key functions in patternin

44 bryonic CNS, mDA originate from the midbrain floor plate, a ventral midline structure that is operati

45 The repulsion is mimicked by a soluble floor plate activity.

46 rious fates in adjacent tissues, such as the floor plate, after the completion of gastrulation.

47 commissural (ILC) axons travel away from the floor plate along an arcuate trajectory into intermediat

48 The floor plate, an essential ventral midline organizing cen

49 We report that Vema is expressed in the floor plate, an intermediate target for pathfinding comm

50 othened is required for induction of lateral floor plate and a subpopulation of hypothalamic cells an

51 e zebrafish Xnot homologue, display enhanced floor plate and adaxial muscle phenotypes.

52 Netrins are secreted by the floor plate and attract commissural axons toward the mid

53 ve rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of

54 10 was highest in the dorsal spinal cord and floor plate and exhibited complex patterning in several

55 t they extend axons that reach and cross the floor plate and express apparently normal levels of the

56 including notochord, prechordal mesendoderm, floor plate and forebrain.

57 We demonstrate here that floor plate and hypochord cells arise from distinct regi

58 Notch signaling promotes floor plate and hypochord fates over notochord, but has

59 ial identity of prechordal plate, notochord, floor plate and hypochord progenitors during gastrulatio

60 thalamic cells and for maintenance of medial floor plate and hypothalamic cells.

61 2 mouse mutants, which lack specifically the floor plate and immediately adjacent interneurons.

62 hevelled at the cilium base in the zebrafish floor plate and in mammalian renal cells.

63 tions, in the presence of a mature notoplate/floor plate and in the absence of the notochord, the cha

64 ution of chick axial progenitor cells to the floor plate and inhibits contribution to the notochord.

65 gehog (Shh) is produced by the notochord and floor plate and is responsible for inducing ventral neur

66 The neurogenic potential of the floor plate and its underlying mechanisms remain unknown

67 f (formerly lefty-1) in the left side of the floor plate and Leftb (formerly lefty-2), nodal and Pitx

68 es of Gli proteins have been performed using floor plate and neuronal induction assays in frog embryo

69 n the notochord lineage, suggesting that the floor plate and notochord arise from distinct precursors

70 that sonic hedgehog (Shh) secreted from the floor plate and notochord is required for specification

71 as detected in the mouse node from where the floor plate and notochord precursors derive.

72 ous work has indicated that signals from the floor plate and notochord promote chondrogenesis of the

73 terns of Nr-CAM in the chick spinal cord and floor plate and on commissural axons, where Nr-CAM has b

74 from nearby organizing centers, such as the floor plate and paraxial mesoderm.

75 al axons grow long distances parallel to the floor plate and precisely maintain their positions using

76 ost-crossing axons allows expulsion from the floor plate and prevents recrossing.

77 dary motoneurons, but the presence of medial floor plate and primary motoneurons in smoothened mutant

78 os are cyclopean, show a significant loss of floor plate and primary motorneurons and display disrupt

79 evelopment, inferior olivary axons cross the floor plate and project from the caudal to the rostral h

80 t ntl function is required to repress medial floor plate and promote notochord fate in cells of the w

81 tube defects revealed the absence of lateral floor plate and secondary motoneurons, but the presence

82 -associated protein Vema is localized to the floor plate and the optic chiasm, intermediate targets l

83 at delimit motor axon trajectories, i.e. the floor plate and the rhombic lip.

84 ate boundaries, at the interface between the floor plate and the ventral funiculus.

85 e cyclops mutation leads to a loss of medial floor plate and to severe deficits in ventral forebrain

86 i3 requires endogenous Gli1 for induction of floor plate and V3 interneurons.

87 w that ephrin-B ligands are expressed in the floor plate and within a dorsal region of the embryonic

88 urce of Sonic Hedgehog (SHH) by ablating the floor plate and/or notochord, or inhibiting SHH function

89 erned in this axis as there is a roof plate, floor plate, and differing numbers and types of neurobla

90 jected their axons longitudinally within the floor plate, and failed to reach their normal exit point

91 h the transverse plane, perpendicular to the floor plate, and in the longitudinal plane, parallel to

92 issural axons before and as they crossed the floor plate, and Netrin-1 stimulation dramatically incre

93 easing anterior-to-posterior gradient in the floor plate, and that a directed source of Wnt4 protein

94 of interneurons that send their axons to the floor plate, and the NGN2 progenitors in the ventral dom

95 Sonic hedgehog (Shh) from the notochord and floor plate appears to generate a ventral-to-dorsal grad

96 hat are absent in Shh mutants, including the floor plate, are present in Shh opb double mutants.

97 gehog (Shh) secreted by the notochord and/or floor plate as a primary regulator of auditory cell fate

98 ges 13-14), will independently mature into a floor plate as assayed three criteria: (1) continued exp

99 Neuregulin 1 (NRG)-1, localized in the floor plate as well as in motor and sensory neurons, is

100 fate specification of the notochord and the floor plate, as well as signaling within and between the

101 n bilateral stripes just dorsolateral to the floor plate at E12.5.

102 Commissural neurons project axons across the floor plate at the spinal cord ventral midline.

103 pioneer a circumferential trajectory to the floor plate at the ventral midline directed by secreted

104 The establishment of the floor plate at the ventral midline of the CNS is depende

105 Induction of the floor plate at the ventral midline of the neural tube is

106 Here we present a novel floor-plate-based strategy for the derivation of human D

107 opriate attraction to Netrin produced by the floor plate because of an accumulation of DCC receptor f

108 s notochord within a population of notochord/floor plate bipotential cells through negative transcrip

109 s ephrin protein is expressed at the lateral floor plate boundaries, at the interface between the flo

110 matically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish.

111 ract pre-crossing axons toward the hindbrain floor plate, but is active in post-crossing guidance.

112 spinal cord are guided ventrally toward the floor plate, but subsequently cross the midline and sele

113 actions also resulted in an avoidance of the floor plate, but without inducing growth-cone collapse.

114 showed that disruption of the notochord and floor plate by diphtheria toxin (DTA)-mediated cell abla

115 and Notch signaling in specification of the floor plate, by late inductive or early allocation mecha

116 This result demonstrates that while the floor plate can serve as a source of long-range cues for

117 These results show that the notoplate/floor plate capacity to induce the medially directed mot

118 nduced transcription factor FoxA2 to specify floor plate cells and dorsally in combination with BMP s

119 heir receptors are reciprocally expressed on floor plate cells and longitudinally projecting axons in

120 uroepithelium in positions characteristic of floor plate cells and motor neurons.

121 ck spinal cord netrin-1 mRNA is expressed by floor plate cells and netrin-2 mRNA by neural epithelial

122 os results in the ectopic differentiation of floor plate cells and ventral neurons within the neural

123 Floor plate cells are a population of epithelial cells t

124 Floor plate cells are induced by sonic hedgehog (SHH) se

125 t interactions between commissural axons and floor plate cells are required to modulate the localizat

126 Notochord and floor plate cells are sources of molecules that pattern

127 i2 and Gli3 repress the ectopic induction of floor plate cells by Gli1 in co-injection assays and inh

128 s the only Gli gene expressed in prospective floor plate cells of frog embryos, we have investigated

129 Foxj1 in the formation of long motile cilia, floor plate cells produce cilia that are longer than the

130 specification but only reduced the number of floor plate cells that developed compared to control emb

131 demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration.

132 eedback loop triggered by Shh that restricts floor plate cells to the midline.

133 SCF is expressed by midline floor plate cells, and its receptor Kit, a receptor tyro

134 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.

135 lic dopamine cell origin from shh expressing floor plate cells.

136 rganized by Sonic hedgehog (Shh) secreted by floor plate cells.

137 only Gli1 can induce the differentiation of floor plate cells.

138 eural tube expressed FP-1, characteristic of floor plate cells.

139 es that correlated with remnants of apparent floor plate cells.

140 he basolateral, but not apical, membranes of floor plate cells.

141 to many notochord cells overlaid by a row of floor-plate cells.

142 In the embryonic spinal cord, the floor plate chemoattractant Netrin-1 is required to guid

143 Although hindbrain floor plate cilia are disorganized in vangl2 mutant embr

144 transient Shh signals from the notochord and floor plate confer a competence in somitic tissue for su

145 Thus, pioneer axons depend on long-range floor plate cues, with Slit/Robo signaling required for

146 neurons whose generation is specified by the floor plate-derived morphogenic signal sonic hedgehog (S

147 hed online at Nature-independently show that floor plate-derived netrin-1 is dispensable for commissu

148 Our results show that floor plate-derived neuropilin-2 is developmentally regu

149 We discovered that a novel source of floor plate-derived, but not axon-derived, neuropilin-2

150 Robo3.2 translation is induced by floor-plate-derived signals as axons cross the spinal co

151 d its midline derivatives, the notochord and floor plate, develop normally in both categories of muta

152 ated by the Ntl protein acting to antagonize floor plate development as well as to promote notochord

153 rmation and promoting hypochord and possibly floor plate development, but the precise mechanisms by w

154 ingly in ntl or spt enhance posterior medial floor plate development.

155 al mesoderm cells is not a pre-requisite for floor plate differentiation and suggest parallels betwee

156 1 and Shh may define the lateral boundary of floor plate differentiation.

157 n co-injection assays and inhibit endogenous floor plate differentiation.

158 lls and mediates the effects of Shh inducing floor plate differentiation.

159 Ectopic induction or genetic deletion of the floor plate diverted longitudinal axons into abnormal tr

160 The definition of the floor plate domain, therefore, appears to be defined by

161 ngly, mAb SAC1 also labels the notochord and floor plate during most stages of spinal cord developmen

162 Since Slits are expressed in the septum and floor plate during the period when these tissues cause c

163 wo organizers: sonic hedgehog (Shh) from the floor plate (DV) and Fgf8 from the isthmus (AP).

164 og (Shh) expression through binding to a Shh floor plate enhancer (SFPE2).

165 comparisons narrowed the activity of the Shh floor plate enhancer to an 88-bp sequence within intron

166 are each required for activation of the Shh floor plate enhancer, whereas the Tbx site was required

167 he early neural midline, the future anterior floor plate, exists as a separate population of floor pl

168 Semaphorins and Slits, which regulate their floor plate exit and restrict their post-crossing trajec

169 cues of the Slit family were altered in the floor plate experiments, suggesting their involvement in

170 prevented the growth cones from entering the floor-plate explants.

171 Foxa2 also regulates the expression of floor plate factors that control axon trajectories aroun

172 ted with differentiation of these cells to a floor plate fate reveals a role for the nascent prechord

173 h Shh to induce the epiblast precursors to a floor-plate fate.

174 ong period when their axons extend along the floor plate, following which they develop additional tro

175 than one inductive event is associated with floor plate formation along the length of the neuraxis.

176 To begin to reconcile models of floor plate formation in the vertebrate neural tube, we

177 n node and notochord, and can induce ectopic floor plate formation when misexpressed in the developin

178 re required non cell-autonomously for medial floor plate formation, suggesting that an inducing signa

179 ue development, likely prior to notochord or floor plate formation.

180 o a repulsive cue expressed at the embryonic floor plate (FP) and also the injured adult CNS.

181 Netrin1 has been proposed to act from the floor plate (FP) as a long-range diffusible chemoattract

182 e spinal cord, ephrin-B3 is localized to the floor plate (FP) at the ventral midline (VM), ephrin-B1

183 vels, Shh produced by both the notochord and floor plate (FP) diffuses dorsally to organize patterned

184 The floor plate (FP) is a midline signaling center, known to

185 gehog (SHH) source in the limb, the SHH-rich floor plate (FP) is reduced in the talpid2 midbrain.

186 the developing nervous system including the floor plate (FP) of the spinal cord where its function i

187 ow towards the midline in the absence of the floor plate (FP), a glial structure occupying the midlin

188 mmissural axons extend toward and across the floor plate (FP), an intermediate target at the ventral

189 gside or significant distances away from the floor plate (FP).

190 midline, send axons ventrally and across the floor plate (FP).

191 s from dorsal spinal cord to ventral midline floor plate (FP). FP cells produce a chemoattractive act

192 idline and functionally resemble the midline floor plate glia of the vertebrate spinal cord.

193 n in craniofacial cartilage, ear, notochord, floor plate, hypochord and fins in a pattern similar to

194 Nevertheless, floor plate identity and ciliogenesis are unaffected in

195 est parallels between the development of the floor plate in amniote and anamniote embryos.

196 pathfinding on the contralateral side of the floor plate in higher vertebrates.

197 While the role of the notochord and floor plate in patterning the dorsal-ventral (D/V) axis

198 t understanding the development of the early floor plate in the chick embryo.

199 pathfinding on the contralateral side of the floor plate in the embryonic rodent spinal cord.

200 is required for specification of the lateral floor plate in the frog.

201 ations in the ventral midline, including the floor plate in the spinal cord, the hindbrain and midbra

202 roper navigation of commissural axons to the floor plate in the spinal cord.

203 in guidance of commissural axons across the floor plate in the spinal cord.

204 e additional chemoattractant activity of the floor plate in vitro and can act directly as a chemoattr

205 e additional chemoattractant activity of the floor plate in vitro and for the normal projection of co

206 ormal projection of commissural axons to the floor plate in vivo.

207 ocalization within the basement membrane and floor plate in vivo.

208 We also find that the medial floor plate in Xenopus comprises two distinct population

209 The midline is similar to the vertebrate floor plate, in that it plays an essential role in cell

210 hat are generated in the region flanking the floor plate, including dopaminergic and serotonergic neu

211 nferior olivary axons; prior exposure to the floor plate increases responsiveness to these cues.

212 pts neurulation by permitting more extensive floor plate induction by Shh, thereby inhibiting midline

213 To understand molecularly how floor plate induction proceeds we identified a Shh-respo

214 required for axons to project away from the floor plate into the lateral funiculus.

215 For many axons, the floor plate is a source of long- and short-range guidanc

216 mpared to that of floating head mutants; the floor plate is almost complete and an anterior notochord

217 the notable exception that posterior medial floor plate is completely absent.

218 The floor plate is known to be a source of repellent signals

219 ord does not develop, and in cyc mutants the floor plate is nearly entirely missing.

220 t the mouse midbrain, but not the hindbrain, floor plate is neurogenic, giving rise to dopamine (DA)

221 Finally, we show that the floor plate is not required for the early trajectory of

222 edgehog (Shh), produced by the notochord and floor plate, is proposed to function as an inductive and

223 rise in close proximity to the notochord and floor plate, it has been assumed that their development,

224 r, our study shows that a subset of anterior floor plate-like cells gives rise to Fgf3(+) SOX3(+) pro

225 We show that the downregulation of Shh in floor plate-like cells in the forebrain governs their pr

226 The mesodiencephalic floor plate (mdFP) is the source of diverse neuron types

227 ural axons are observed crossing the ventral floor plate midline perpendicularly at about 20 microns/

228 ngly, our studies identified a rostral brain floor plate Neurog2 enhancer that requires direct input

229 ta-catenin signaling and Shh may orchestrate floor plate neurogenesis or a lack thereof.

230 evelopment of the vertebrate dorsal midline (floor plate, notochord, and hypochord) has been an area

231 floorplate, is not found in the prospective floor plate of nt embryos.

232 In cells of the neural tube floor plate of p190 RhoGAP mutant mice, polymerized acti

233 let1(+) motor neuron cell bodies invaded the floor plate of Robo1/2 double mutant mouse embryos or Sl

234 are thought to arise from progenitors in the floor plate of the caudal diencephalon and midbrain.

235 dance of commissural growth cones across the floor plate of the embryonic chicken spinal cord.

236 In addition, netrin-1 mRNA is found in the floor plate of the embryonic nervous system, an intermed

237 ce cue expressed in the ventricular zone and floor plate of the embryonic neural tube.

238 2-regulated enhancers that are active in the floor plate of the midbrain or along the length of the e

239 h it is expressed in an adjacent tissue: the floor plate of the nerve cord.

240 FOXA2, which marks the floor plate of the neural tube and definitive endoderm,

241 t for patterning of the ectodermally derived floor plate of the neural tube and the mesodermally deri

242 e notochord, dorsal foregut, and part of the floor plate of the neural tube.

243 and was coexpressed with Shh in the ventral floor plate of the neural tube.

244 n's node such as endoderm, notochord and the floor plate of the neural tube.

245 alisation with that of Sonic hedgehog in the floor plate of the neural tube.

246 from one of their intermediate targets, the floor plate of the spinal cord.

247 ute two orthogonal turns before crossing the floor plate or extend directly across the floor plate.

248 s on the presence of either a differentiated floor plate or notochord.

249 itor cells that can contribute to either the floor plate or the notochord.

250 nmental factors in the ventricular zone, the floor plate, or other tissues coordinate its function.

251 or plate, exists as a separate population of floor plate precursor cells in the epiblast of the gastr

252 Midbrain floor-plate precursors are derived from PSCs 11 days aft

253 The floor plate produces several types of guidance cues with

254 anding large numbers of homogeneous midbrain floor plate progenitors (mFPPs) that retain efficient DA

255 aled that Notch signaling maintains dividing floor plate progenitors.

256 l commissural (MLC) axons grow alongside the floor plate, projecting primarily in the rostral directi

257 , including stalling and knotting inside the floor plate, recrossing, randomized anterior-posterior p

258 ) specifies a ventral progenitor fate in the floor plate region that later gives rise to mDA neurons.

259 e for conferring proper cell identity in the floor-plate region of midbrain and does not require an O

260 n post-crossing commissural axons to mediate floor plate repulsion in the mouse spinal cord.

261 al axon navigation across the midline in the floor plate requires repulsive forces from local Slit re

262 motor axons when co-cultured with septum or floor plate respectively.

263 placement of the notochord from the midbrain floor plate resulted in abnormal folding and overall col

264 In the mouse cochlea and in the zebrafish floor plate, Rpgrip1l was required for positioning the b

265 , prechordal plate, notochord, hypochord and floor plate share a common embryonic origin.

266 ional transcription factors expressed in the floor plate share overlapping functions with Foxj1.

267 the ventral midline of the neural tube, the floor plate, share the property of being a source of the

268 t is under positive control by notochord and floor plate (Shh), dorsal neural tube (Wnt1) and surface

269 ultures, olivary axons which had crossed the floor plate showed an increased tendency to respond to c

270 transiently at the contralateral edge of the floor plate, showing a delay in midline exit.

271 ts support the view that either notochord or floor plate signaling can specify Lim3-positive motoneur

272 Floor plate-specific deletion of neuropilin-2 significan

273 F-spondin; (2) the display of the notoplate/floor plate-specific randomly oriented protrusive activi

274 otochord decisions and plays a minor role in floor plate specification, where it acts in parallel to

275 ally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role

276 he expression of all three Slit genes in the floor plate suggests that they are likely to share the s

277 gical and functional features of the amniote floor plate that distinguish these cells from the rest o

278 experiments, we showed that in the zebrafish floor plate the function of Rpgrip1l in planar polarity

279 lf extends many cell diameters dorsal to the floor plate, the site of netrin-1 expression.

280 though Slits are expressed in the septum and floor plate, their proteins do not contribute to the maj

281 e hindbrain roof plate; and that, unlike the floor plate, these dorsal organizing centers develop in

282 We have found that the floor plate throughout the midbrain, hindbrain and spina

283 ance mechanisms in vivo, the overall role of floor plate tissue and the specific roles of Slit/Robo s

284 Here, we show that notochord or floor plate tissue can induce the formation of ectopic s

285 ord, sonic hedgehog (Shh) is secreted by the floor plate to control the generation of distinct classe

286 Commissural filopodia appear on the floor plate to interact with the nascent neural network,

287 (SCO)-spondin-GFP protein secretion from the floor plate to merge with the fiber.

288 though commissural axons readily entered the floor plate under control conditions, perturbations of e

289 After initial attraction to the floor plate using Netrin1 activation of its main attract

290 Likewise, signals from notochord and floor plate ventralise the somite, at high levels overri

291 find that Shh signaling is not required for floor plate vs. notochord decisions and plays a minor ro

292 f hypochord at the expense of notochord, but floor plate was not affected.

293 st establish that the notoplate (presumptive floor plate), when separated from the underlying notocho

294 loping neural tube, but is excluded from the floor plate where Sonic hedgehog (Shh) is expressed.

295 spinal cord send axons ventrally toward the floor plate, where they cross the midline and turn anter

296 f Shh decreased locally in the notochord and floor plate, whereas overt patterning and differentiatio

297 we cocultured cortical explants with E13 rat floor plate, which expresses Netrin-1, or with Netrin-1-

298 be Cdo is expressed within the Shh-dependent floor plate while Boc expression lies within the dorsal

299 y 1/2 and activation of SMADs 2 and 3 at the floor plate, while cell fate specification of the notoch

300 n and spinal cord despite the absence of the floor plate, while no tail mutant embryos, which lack a