ライフサイエンスコーパス: flow cytometry

1 rus-specific T cells (quantified by cytokine flow cytometry).

2 3CD14CD15HLA-DR (monocytes), were defined by flow cytometry.

3 yzed by histology, immunohistochemistry, and flow cytometry.

4 by in situ hybridization for runx1/cmyb and flow cytometry.

5 ace P-selectin levels were measured by using flow cytometry.

6 emokine receptor expression were analyzed by flow cytometry.

7 rom submandibular lymph nodes as observed by flow cytometry.

8 hoid cells (ILC) from peripheral blood using flow cytometry.

9 ta3 expression in individual cells simply by flow cytometry.

10 d protists in the BW, which was supported by flow cytometry.

11 ion molecule PECAM-1 (CD31) when examined by flow cytometry.

12 Neutrophil viability was assessed by flow cytometry.

13 aphy, quantitative immunohistochemistry, and flow cytometry.

14 tion and regulatory T cells, was explored by flow cytometry.

15 ied and characterized from plasma samples by flow cytometry.

16 tage of nucleoprotein 1-positive cells using flow cytometry.

17 X and HDs exosomes were evaluated by on-bead flow cytometry.

18 typing at throughputs comparable to those of flow cytometry.

19 d markers of degranulation and activation by flow cytometry.

20 nt T cells in RM from 69 HIV-negative men by flow cytometry.

21 cell analyses and techniques such as imaging flow cytometry.

22 to evaluate T and NK cells reconstitution by flow cytometry.

23 he advantages of fluorescence microscopy and flow cytometry.

24 retinal wholemounts and cryosections and by flow cytometry.

25 )R-expressing Chinese hamster ovary cells by flow cytometry.

26 CD20 expression was measured by flow cytometry.

27 BP) Ab to visualize granules and assessed by flow cytometry.

28 f C1q and C4b from serum when analyzed using flow cytometry.

29 opy with high sample numbers associated with flow cytometry.

30 nd intranuclear) markers were assessed using flow cytometry.

31 no detectable residual disease (RD; 84%) by flow cytometry.

32 of hamster and human origin was confirmed by flow cytometry.

33 heral blood CD4(+) T cells was quantified by flow cytometry.

34 tein (ENV) or GAG peptides by multiparameter flow cytometry.

35 ferase gene-knockout (GTKO), and TKO pigs by flow cytometry.

36 generation by histology, RNA sequencing, and flow cytometry.

37 ing and phenotypes of T cells in blood using flow cytometry.

38 ive immune cell subsets using multiparameter flow cytometry.

39 ion of cell surface epitopes was analyzed by flow cytometry.

40 al barrier (polarized BeWo monolayers) using flow cytometry.

41 s CD4+ cells that can be readily detected by flow cytometry.

42 feration and cytokine secretion assays or by flow cytometry.

43 R, and the accuracy is comparable to that of flow cytometry.

44 typing of peripheral lymphocytes was done by flow cytometry.

45 te was evaluated by immunohistochemistry and flow cytometry.

46 be detected using both confocal imaging and flow cytometry.

47 xP3 (Forkhead Box P3) protein as detected by flow cytometry.

48 helper (T(FH)) cell subsets was analyzed by flow cytometry.

49 of blood leukocyte and lymphocyte subsets by flow cytometry.

50 thin live cells with confocal microscopy and flow cytometry.

51 nses in lymphoid tissues were confirmed with flow cytometry.

52 or distilled water (control) and analyzed by flow cytometry.

53 ression on CD4+ T cells was determined using flow cytometry.

54 ts using intracellular cytokine staining and flow cytometry.

55 ined using real-time PCR, immunoblotting and flow cytometry.

56 ng, real-time polymerase chain reaction, and flow cytometry.

57 for C3 detection in immunohistochemistry and flow cytometry.

58 oduction and CD107a membrane accumulation by flow cytometry.

59 antifying the T-cell frequency and number by flow cytometry.

60 ry, real-time polymerase chain reaction, and flow cytometry.

61 s by 50% as measured by Western blotting and flow cytometry.

62 ng CD38, HLADR, and/or Ki67 were assessed by flow cytometry.

63 tissues were analyzed by RNA sequencing and flow cytometry.

64 say and neutrophil survival was analyzed via flow cytometry.

65 tested using extracellular flux analysis and flow cytometry.

66 retion profiles and cellular polarization by flow cytometry.

67 ypes and plasma cytokines were determined by flow-cytometry.

68 ma by ExoQuick solution and characterized by flow-cytometry.

69 Utilizing flow cytometry, adoptive cell therapy and genetic approa

70 naive) CD154+ CD4+ T cells were analyzed by flow cytometry after 5 hours of stimulation with 200 umo

71 ST2 expression on lung ILC2 were measured by flow cytometry after treatment of rTSLP, rIL-33, rTSLP +

72 ce and intracellular cytokine phenotyping by flow cytometry along with serum antibody testing in 18 k

73 Flow cytometry, an automated technique for measuring and

74 Confocal microscopy and flow cytometry analyses showed efficient transfection ef

75 Flow cytometry analyses showed that more than 78% (HeLa)

76 Flow cytometry analysis demonstrated specific binding of

77 Imaging flow cytometry analysis demonstrated that the frequency

78 ress these issues, we have developed a novel flow cytometry analysis pipeline to identify a plethora

79 Flow cytometry analysis revealed a 2C-DNA value of 3.8 +

80 The flow cytometry analysis revealed that cellular uptake an

81 Flow cytometry analysis showed substantially increased n

82 Accordingly, the flow cytometry analysis showed that the counts of a subs

83 Flow cytometry analysis showed that the lipid accumulate

84 The results from logic-gated flow cytometry analysis was validated with bioinformatic

85 uantitative polymerase chain reaction assay, flow cytometry analysis, and Western blotting were appli

86 elial cell transduction based on imaging and flow cytometry analysis.

87 Blood lymphocytes were quantified by flow cytometry and antigen specificity by in vitro cytok

88 nt properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography.

89 ILC (ILC3) and NK cells using polychromatic flow cytometry and cell stimulation assays in colon, ton

90 rom pancreatic cancer cell populations using flow cytometry and characterized by tumor sphere formati

91 Immune reconstitution was monitored through flow cytometry and CMV viremia was tracked via quantitat

92 Cytotoxicity studies, clonogenic assay, flow cytometry and confocal imaging were conducted to ev

93 Flow cytometry and confocal microscopy detected the near

94 Using immunofluorescent labeling, flow cytometry and Cre-dependent ribosomal immunoprecipi

95 onstrate a novel technique combining imaging flow cytometry and cross-polarised light (ISX(+PL)) to r

96 Flow cytometry and cytokine measurements in bronchoalveo

97 we demonstrate the use of label-free imaging flow cytometry and deep learning to characterize RBC les

98 Further analysis with qPCR, flow cytometry and ELISA experiments revealed that GM-CS

99 Here, using flow cytometry and ELISA we show that GM-CSF induces an

100 controls was analyzed by techniques based on flow cytometry and enzyme-linked immunosorbent assays.

101 rom peripheral blood mononuclear cells using flow cytometry and enzyme-linked immunospot assays.

102 ls in Spodoptera frugiperda (Sf9) culture by flow cytometry and evaluating GPCR stability by size-exc

103 By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow

104 d mononuclear cells from MOG-AAD patients by flow cytometry and found a strong antigen specific centr

105 patches and lamina propria were analyzed by flow cytometry and IgA repertoire was determined by next

106 bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it s

107 cepted kidney allografts were analyzed using flow cytometry and immunohistochemical staining.

108 Flow cytometry and immunohistochemistry experiments on t

109 Flow cytometry and immunohistochemistry were used to ass

110 ped from their low-dimensional counterparts, flow cytometry and immunohistochemistry, to meet this ne

111 za-specific TFH-cell responses after LAIV by flow cytometry and immunohistochemistry.

112 Using flow cytometry and immunohistology, we found that CD26,

113 d patients with IPEX syndrome were tested by flow cytometry and in vitro suppression assays, and the

114 CD8(+) T-cell responses were measured using flow cytometry and intracellular cytokine staining and c

115 be used for the detection of TNF cleavage in flow cytometry and live-cell imaging applications.

116 e present exhibited decreased granularity by flow cytometry and marked depletion of intracytoplasmic

117 omosomes from metaphase-arrested cells using flow cytometry and perform LC-MS/MS to identify chromoso

118 rified ISCs and intestinal progenitors using flow cytometry and performed RNA-sequencing analysis.

119 ous T-cell lymphoma is typically assessed by flow cytometry and plays a critical role in diagnosis, c

120 Flow cytometry and qPCR further analyzed ex vivo the glo

121 PD-L1 and PD-L2 expression was assessed by flow cytometry and qRT-PCR in brain tumor cell lines and

122 Using flow cytometry and quantitative Polymerase Chain Reactio

123 s of the receptor's alpha-chain, analyzed by flow cytometry and quantitative RT-PCR.

124 nsplant DOCK8-deficient patients (n = 12) by flow cytometry and real-time qPCR.

125 d anaerobic (-195 +/- 15 mV) conditions, and flow cytometry and selective plating were used to quanti

126 Flow cytometry and sorted-blood-cell RNA-seq in addition

127 creased during flares in all 4 patients, and flow cytometry and sorted-cell RNA-seq confirmed the pre

128 We combine sequencing, flow cytometry and sorting, followed by microscopy to mo

129 combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to descri

130 Cell fate assays showed that multicolor flow cytometry and transcriptional profiling successfull

131 d Brazilian ZIKV isolate and used multicolor flow cytometry and transcriptional profiling to describe

132 essed by functional assays in vitro, whereas flow cytometry and transcriptomic analysis were used to

133 Using flow cytometry and Western blot analysis, we observed th

134 mounts are significantly reduced as shown by flow cytometry and Western blotting, using strains expre

135 ls and NK cells isolated from the spleen (by flow cytometry) and the presence of macrophages (Iba-1 p

136 ropsy, histologic examination, IHC analysis, flow cytometry, and advanced imaging.

137 ranscriptase quantitative PCR, intracellular flow cytometry, and ELISA.

138 damage by confocal microscopy, cell cycle by flow cytometry, and homologous recombination (HR) by a G

139 cell-based reporter assays, genome editing, flow cytometry, and immunofluorescence microscopy.

140 re profiled with single-cell RNA sequencing, flow cytometry, and immunofluorescence, with IL-5Ralpha

141 ere characterized by cytokine production and flow cytometry, and in a subset of children RNA sequenci

142 Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan pr

143 ining, knockdown and overexpression studies, flow cytometry, and luciferase reporter assays.

144 Fluorescence microscopy, flow cytometry, and PCR were performed to determine the

145 n by IL-7, IL-23, and IL-12 by cell culture, flow cytometry, and phospho-flow assays.

146 slit-lamp microscopy, immunohistochemistry, flow cytometry, and polymerase chain reaction.

147 D4+ and CD8+ T-cell subsets were measured by flow cytometry, and prevalent diabetes cases were adjudi

148 rity by immunocytochemistry, immunoblotting, flow cytometry, and real-time PCR to quantify gene expre

149 ed using immunohistochemistry, polychromatic flow cytometry, and reverse transcription-PCR.

150 T cells were analyzed by flow cytometry, and serum amyloid proteins (SAA) were an

151 re quantified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were m

152 on were evaluated using confocal microscopy, flow cytometry, and the flexiVent small-animal ventilato

153 unction were analyzed using high-dimensional flow cytometry, and the obtained data were compared with

154 immunoblotting, immunohistochemical testing, flow cytometry, and transcriptome and cytokine profiling

155 By using a multicolor flow cytometry approach to analyze and characterize diff

156 xpression within a comprehensive single-tube flow cytometry assay effectively overcomes interpretativ

157 We present a microsphere-based flow cytometry assay that quantifies the ability of plas

158 4-IgG and MOG-IgG by using a live-cell-based flow cytometry assay.

159 Using a fluorescent timer and flow cytometry-assisted organelle sorting, Yau et al. de

160 ies and cytokine expression were measured by flow cytometry at day 3 postinfection.

161 n to ECs (CLDN5-GFP hPSC-ECs), and performed flow cytometry-based chemogenomic library screening to m

162 We conducted a comprehensive phenotypic flow cytometry-based longitudinal analysis of the periph

163 -reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected ind

164 ns permits the use of immunofluorescence and flow cytometry-based methodologies to unambiguously trac

165 A flow cytometry-based phenotypic study and quantification

166 cell RNA and antigen receptor sequencing and flow cytometry-based validation.

167 easurable residual disease (MRD) assessed by flow cytometry before alloSCT as a strong predictor of r

168 cell profiling was performed by whole blood flow cytometry: CD4(+) T cells, Th2 cells (CD4(+) CRTh2(

169 Flow cytometry (CD45.1/45.2) demonstrated abundant blood

170 Flow cytometry, cell culturing, and microscopy may reach

171 ir functional properties were analyzed using flow cytometry, cell kinetic studies, co-culture with CD

172 allowing potential cross-referencing between flow cytometry, cellular indexing of transcriptomes and

173 s breast epithelial cells of luminal origin, flow cytometry characterization, and genomic sequencing,

174 ations in organoids using immunostaining and flow cytometry; cholangiocyte proliferation of cholangio

175 erculosis-specific CD4 T cells detectable by flow cytometry, combined with overall elevated T-cell ac

176 Imaging flow cytometry combines the morphological information pr

177 ation in DLB using multiplex immunoassay and flow cytometry concomitantly.

178 Finally, flow cytometry confirmed low-level engraftment of BM cel

179 ibody testing, autoreactive and alloreactive flow cytometry crossmatches (FXM) using traditional FXM

180 the CSA in combination with multiparametric flow-cytometry (CSA-Flow) may enable simultaneous isolat

181 Flow cytometry data are traditionally analyzed by (subje

182 Analysis of flow cytometry data reconstructed a detailed map of baso

183 he single-cell RNA sequencing work to recent flow cytometry data suggesting a strong atheroprotective

184 developed tools for single-cell analysis on flow cytometry data, as well.

185 rogeneity and intercellular relationships in flow cytometry data.

186 and additional prerequisites when applied on flow cytometry data.

187 Flow cytometry demonstrated a 4.5-fold increase in CX3CR

188 Flow cytometry demonstrated decreased numbers of CD45+ c

189 Flow cytometry demonstrated specific expression of CCR2

190 Quantitative, multicolor flow cytometry during a variety of NK cell activation co

191 Retinal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31(+) CD45(-)

192 Cytokine production was measured using flow cytometry, ELISA, RNA in situ hybridization and qua

193 BAL before and 3 days after challenge using flow cytometry, ELISA, RNA sequencing, and mass spectrom

194 Flow cytometry, ELISAs, cocultures, intracellular staini

195 B-cell subsets, and DSA were measured using flow cytometry; expression of cytokines and costimulator

196 o binding constants from radioligand binding/flow cytometry; fast association/dissociation (~2 min)]

197 igh-content multi-parameter phospho-specific flow cytometry, fluorescent cell barcoding and automated

198 genous CD4 and tetherin was quantified using flow cytometry following transfection into an immortaliz

199 temness was examined with salisphere assays, flow cytometry for ALDH/CD44 (CSC markers for MEC), and

200 at cerebellar atrophy is seen in other IGDs, flow cytometry for GPI-APs should be considered in the w

201 or the first time, to our knowledge, imaging flow cytometry for investigating interactions of represe

202 o ovalbumin-stimulated PBMC were analyzed by flow cytometry for presence of ovalbumin-specific regula

203 atients were characterized phenotypically by flow cytometry for single-cell resolution of distinct IL

204 s presented here suggest that large-particle flow cytometry has the potential to significantly increa

205 We performed multicolor flow cytometry, high-coverage single-cell RNA sequencing

206 We used flow cytometry, histology, and immunofluorescence to cha

207 IU/ml (HBs(hi)) using immunological assays (flow cytometry, ICS, ELISPOT).

208 tometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for

209 We have employed advanced imaging flow cytometry (iFCM) to explore the kinetics of allogra

210 The early analysis of progenitors using flow cytometry, immunocytochemistry, and qRT-PCR showed

211 Flow cytometry, immunocytochemistry, immunofluorescence,

212 umber, proliferation, and differentiation by flow cytometry, immunofluorescence, and organoid assays.

213 les) and in kidney podocytes was mapped with flow cytometry, immunoprecipitation, and trypanolysis as

214 Using ELISA, flow cytometry, immunoprecipitation, mass spectrometry,

215 In this study we used nanoscale flow cytometry in conjunction with an engineered FRET re

216 es in blood and cerebrospinal fluid (CSF) by flow cytometry in HIV-infected adults with cryptococcal

217 Using high-dimensional flow cytometry, in vitro assays, and RNA sequencing, we

218 Flow cytometry indicated that the IL22 was produced prim

219 Currently, flow cytometry is the gold standard for biomedical multi

220 Here, we used multidimensional flow cytometry (MFC) to prospectively screen for the pre

221 able residual disease (MRD) was monitored by flow cytometry (MFC-MRD) and correlated with outcome.

222 se cells based only on surface markers using flow cytometry might not provide a full phenotypic pictu

223 In mice treated with Leuko-Rapa, flow cytometry of disaggregated aortic tissue revealed f

224 Flow cytometry of dispersed tissue fragments and serial

225 These include flow cytometry of endogenously expressing cells, real-ti

226 Flow cytometry of the five most highly abundant peptides

227 Comprehensive flow cytometry of whole blood samples from 54 COVID-19 p

228 Flow-cytometry of allografts revealed liposome presence

229 Using flow cytometry on peripheral blood mononuclear cells and

230 Multiparametric flow cytometry on peripheral blood T cells was performed

231 Histology and flow cytometry on spleens and thymi from 3-week-old pups

232 n NRE PicoP abundances and composition using flow cytometry, over a 1.5 year period.

233 lyzed them using a high-dimensional spectral flow cytometry panel and confirmed our findings by confo

234 Using a flow cytometry panel to assess cellular phenotypes, mRNA

235 We show that imaging flow cytometry provided comprehensive population-based s

236 verall, this study demonstrates that imaging flow cytometry provides powerful means for disclosing po

237 Flow cytometry, quantitative PCR, next-generation sequen

238 cirrhosis (19 with SBP) and analyzed them by flow cytometry, quantitative real-time polymerase chain

239 multiple mouse tumor and viral models using flow cytometry, quantitative reverse-transcriptase PCR (

240 meters measured by conventional and spectral flow cytometry reinforces the need to apply many of the

241 y cytokine multiplex detection, qRT-PCR, and flow cytometry respectively.

242 lls were assessed by biophotonic imaging and flow cytometry, respectively.

243 luated by the MTS assay, Ki-67 staining, and flow cytometry, respectively.

244 Using a cut-off rule based on flow cytometry results of the negative control CD154-enr

245 Flow cytometry results showed that a significantly great

246 ese differences, quantification of spread by flow cytometry revealed remarkably similar spread effici

247 Flow cytometry revealed that chlamydial infection induce

248 Immunofluorescence microscopy and flow cytometry revealed that Langerin-AhR(-/-) but not C

249 feration and cytokine secretion assays or by flow cytometry, RNA sequencing, or real-time quantitativ

250 nsively analyzed the brain TME landscape via flow cytometry, RNA sequencing, protein arrays, culture

251 Poststroke inflammation was evaluated with flow cytometry, RT-PCR, MultiPlex, and immunofluorescenc

252 Results: Quantitative flow cytometry showed consistent expression of the NKp30

253 Immunocytochemistry and flow cytometry showed that F302L does not impair KCNA2 s

254 Intravital microscopy via cranial window and flow cytometry showed that in the inflamed brain anti-VC

255 Interestingly, utilization of flow cytometry showed that patients with active pemphigu

256 Combining this strategy with imaging flow cytometry, single-cell RNA sequencing, and maxRNA s

257 ovirus by combining Ifnb1 reporter mice with flow cytometry, single-cell RNA sequencing, confocal mic

258 trols and MM patients using multidimensional flow cytometry; surprisingly, we found that CD11b+CD14-C

259 ontrast, enzyme-linked immunospot (ELISPOT), flow cytometry, time-of-flight mass cytometry (CyTOF), a

260 r this lethal cancer, we applied DNA content flow cytometry to a series of 15 tumor samples including

261 , and fluorophore-based confocal imaging and flow cytometry to confirm EV uptake.

262 We used multiparametric flow cytometry to count 14 subsets of peripheral blood c

263 We used flow cytometry to determine tetraspanins CD9, CD63, and

264 onal immunophenotyping using multiparametric flow cytometry to examine peripheral immune changes unde

265 ombination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically

266 We performed multicolor flow cytometry to investigate CD57(+) FcepsilonRIgamma(n

267 ive RT-PCR, a validated GLP-1R antibody, and flow cytometry to quantify GLP-1R promoter activity, gen

268 of B cell responses through high-dimensional flow cytometry to reveal substantial heterogeneity in bo

269 Subsequently, we used flow cytometry to validate these interactions, and a tot

270 Flow cytometry, total internal reflection fluorescence a

271 their expression of activation markers using flow cytometry, traditional gating-based analysis method

272 In this work, we used a single-cell flow-cytometry trafficking assay to quantitatively exami

273 d performed histology, immunohistochemistry, flow cytometry, transcriptome, and real-time polymerase

274 en-thawed sperm (%ViableSperm) determined by flow cytometry varied from -2.2 in LF to + 7.8 in HF bul

275 ere measured at baseline and, in a subgroup, flow cytometry was performed at weeks 2 and 14.

276 Flow cytometry was performed to characterize CCR2+ cells

277 11-color flow cytometry was performed to determine CD27(+) and CD

278 Flow cytometry was used to analyze CD19(+) B cell subset

279 Flow cytometry was used to determine if lipid oxidation

280 Flow cytometry was used to measure IFN-gamma, IL-9, IL-1

281 Furthermore, flow cytometry was used to measure the frequency of EBV-

282 le cells RNA sequencing and high-dimensional flow cytometry, we demonstrate that T(SCM) heterogeneity

283 Using flow cytometry, we determined galectin-9 expression on i

284 n of the left anterior descending artery and flow cytometry, we first characterized the temporal and

285 mononuclear phagocytes and multidimensional flow cytometry, we found that CP- and ILF-associated CD1

286 Using single-cell RNA sequencing and flow cytometry, we found that miR-155 promotes the activ

287 ing, kinase assays, immunoprecipitation, and flow cytometry, we found that TGFbetaR signaling promote

288 Using high-dimensional flow cytometry, we phenotyped peripheral blood NK cells

289 Spirometry, respiratory symptom tests, and flow cytometry were performed at the same times to asses

290 tion tests, whole blood counts, and platelet flow cytometry were performed.

291 ohistochemistry, 3D confocal microscopy, and flow cytometry were used to characterize a novel mouse m

292 Gene expression profiling and flow cytometry were used to characterize Siglec-8 expres

293 Immunohistochemistry and flow-cytometry were used to determine the presence of pr

294 NA) analyses, complemented by microscopy and flow cytometry, were performed on each sample.

295 sualization of mucin-domain glycoproteins by flow cytometry, Western blot, and immunohistochemistry.

296 nd protein expression were assessed by qPCR, flow cytometry, Western blotting, and immunofluorescence

297 nce lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, im

298 cytosolic delivery efficiency using imaging flow cytometry with cytosolic delivery validated using c

299 Here we coupled multiparameter flow cytometry with lesion-specific photolyases that eli

300 complete white blood cell count, followed by flow cytometry with multiple markers, and cytology.