ライフサイエンスコーパス: fluorescence anisotropy

1 predict the glucose concentrations using the fluorescence anisotropy.

2 human serum albumin (HSA) was studied using fluorescence anisotropy.

3 ng to Fe-Fur and apo-Fur target sequences by fluorescence anisotropy.

4 ithin its matrix, this can result in a large fluorescence anisotropy.

5 aracterized its biophysical properties using fluorescence anisotropy.

6 protein, leading to a strong decrease in the fluorescence anisotropy.

7 mperature regulated high pressure cell using fluorescence anisotropy.

8 ing full-length HMGA1a protein binding using fluorescence anisotropy.

9 ect of nucleotides on this interaction using fluorescence anisotropy.

10 n fluorescence resonance energy transfer and fluorescence anisotropy.

11 d their binding to DNA was characterized via fluorescence anisotropy.

12 e SPR, isothermal titration calorimetry, and fluorescence anisotropy.

13 sed CTD motion or flexibility as measured by fluorescence anisotropy.

14 y tagging receptors and measuring changes in fluorescence anisotropy.

15 re detected and confirmed by competition and fluorescence anisotropy.

16 iation constant measured independently using fluorescence anisotropy.

17 conjugation was measured with time-resolved fluorescence anisotropy.

18 the D1D2 barrel of p97 that was confirmed by fluorescence anisotropy.

19 orescein tag to measure binding affinity via fluorescence anisotropy.

20 hemical analog of fluorescence polarization (fluorescence anisotropy), a versatile optical approach w

21 cently presented one such technique based on fluorescence anisotropy, a spectroscopic method based on

22 Using SEC HPLC and fluorescence anisotropy, absorption spectra were assigne

23 ow micromolar dissociation constants through fluorescence anisotropy after only two rounds of selecti

24 The fluorescence anisotropy, after an initial decay starting

25 -detection size-exclusion chromatography and fluorescence anisotropy allowed us to confirm that two d

26 Surface plasmon resonance and fluorescence anisotropy analyses demonstrated that hCpGA

27 Multisite FRET and fluorescence anisotropy analyses showed that S1 binding

28 tro using electrophoretic mobility shift and fluorescence anisotropy analyses.

29 The same effect is found for the fluorescence anisotropy analysis, for which the trans (c

30 s, electrophoretic mobility shift assay, and fluorescence anisotropy analysis, we determined key amin

31 s-isomer and was directly measurable through fluorescence anisotropy analysis.

32 d KaiA was stronger than normal, as shown by fluorescence anisotropy analysis.

33 omologous domains of p63 and p73 in vitro by fluorescence anisotropy, analytical ultracentrifugation

34 cells and are the active species as shown by fluorescence anisotropy and analytical ultracentrifugati

35 rget DNA sequence have been determined using fluorescence anisotropy and calorimetry.

36 opterin, incorporated into the P1 helix, and fluorescence anisotropy and catalytic activity were meas

37 tion of the RPEL(MAL):G-actin interaction by fluorescence anisotropy and cell reporter-based assays v

38 n binding site on cofilin, but we show using fluorescence anisotropy and chemical crosslinking that i

39 Using fluorescence anisotropy and electrophoretic mobility shi

40 ion of Lsr2 reduced DNA binding, measured by fluorescence anisotropy and electrophoretic mobility shi

41 Using fluorescence anisotropy and exploiting the symmetry of t

42 r such fluorescence changes were examined by fluorescence anisotropy and fluorescence intensity measu

43 Fluorescence anisotropy and fluorescence lifetime measur

44 We used fluorescence anisotropy and FRET imaging of Venus-tagged

45 onsensus RSS versus non-RSS substrates using fluorescence anisotropy and gel mobility shift assays.

46 Analysis of the sensitized fluorescence anisotropy and intensity decays indicates t

47 Fluorescence anisotropy and linear dichroism imaging hav

48 em conjugate via analysis of the time-domain fluorescence anisotropy and NMR chemical shift perturbat

49 The behavior of fluorescence anisotropy and polarization in systems with

50 d NS3, resulting in a hyperbolic increase in fluorescence anisotropy and providing an apparent equili

51 Fluorescence anisotropy and single molecule DNA stretchi

52 states were functionally characterized using fluorescence anisotropy and steady-state kinetics.

53 easurements using microscale thermophoresis, fluorescence anisotropy and surface plasmon resonance ch

54 Fluorescence anisotropy and surface plasmon resonance ex

55 Fluorescence anisotropy and surface plasmon resonance in

56 Time-dependent fluorescence anisotropy and temperature-dependent Forste

57 Using both fluorescence anisotropy and time-resolved fluorescence q

58 parameters from the simultaneous analysis of fluorescence anisotropy and total fluorescence and overc

59 Here, we apply transient-state fluorescence anisotropy and total fluorescence stopped-f

60 oaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (

61 Combining surface plasmon resonance, fluorescence anisotropy, and circular dichroism (CD), we

62 yed an array of conformational probes (FRET, fluorescence anisotropy, and circular dichroism) to reve

63 tro using analytical ultracentrifugation and fluorescence anisotropy, and in living cells using two-p

64 including the fluorescence emission maximum, fluorescence anisotropy, and membrane bilayer penetratio

65 circular dichroism, fluorescence quenching, fluorescence anisotropy, and NMR.

66 Single-turnover kinetics, fluorescence anisotropy, and single-molecule fluorescenc

67 Here, using hydrogen/deuterium exchange, fluorescence anisotropy, and structural analyses, we sho

68 ng UV-vis absorption, fluorescence emission, fluorescence anisotropy, and two-photon absorption (2PA)

69 Using a fluorescence anisotropy approach, PEA-15 is shown to be

70 ry single-molecule fluorescence and ensemble fluorescence anisotropy approaches to discover how NNRTI

71 l mobilities that are inaccessible with bulk fluorescence anisotropy approaches, and anticipate that

72 To this end, we used fluorescence anisotropy as the transduction mechanism to

73 on (binding to TNFalpha mRNA) by an in vitro fluorescence anisotropy assay and to modulate TNFalpha i

74 The dissociation constants obtained in the fluorescence anisotropy assay for binding of all compoun

75 A fluorescence anisotropy assay showed that Arp2 does not

76 Fluorescence anisotropy assay showed that FAM-UNO intera

77 M selectivity, here we developed a real-time fluorescence anisotropy assay to delineate the pMHCII in

78 Here, we have used a fluorescence anisotropy assay to demonstrate that eIF4G

79 del glycoprotein asialofetuin (ASF), using a fluorescence anisotropy assay to measure the concentrati

80 We used a novel fluorescence anisotropy assay to show that the specific

81 Using a competitive fluorescence anisotropy assay, we determined that monoph

82 Using a well established fluorescence anisotropy assay, we tested the direct inte

83 cond-order acylation rate constants with the fluorescence anisotropy assay.

84 binding was further quantified with a novel fluorescence anisotropy assay.

85 ia a virtual screen followed by testing in a fluorescence anisotropy assay.

86 bacterial two-hybrid assay and in vitro in a fluorescence anisotropy assay.

87 MSI family RRM domains using a quantitative fluorescence anisotropy assay.

88 Finally, fluorescence anisotropy assays indicate that Fe-Fur spec

89 Fluorescence anisotropy assays on lambda Cro and the tri

90 this work, we used protein semisynthesis and fluorescence anisotropy assays to explore the interactio

91 ies using electrophoretic mobility shift and fluorescence anisotropy assays.

92 abasic DNA product using both band shift and fluorescence anisotropy assays.

93 CzrA formed complexes in gel-retardation and fluorescence-anisotropy assays with fragments of promote

94 is protein, we observed an unusual, negative fluorescence anisotropy at pH 6.0.

95 cal conformation, and a distinct increase in fluorescence anisotropy attributed to Tyr39 indicates an

96 isoform (p37(AUF1)) as a model, we employed fluorescence anisotropy-based approaches to define therm

97 that a previously reported high-throughput, fluorescence anisotropy-based assay for ATP-dependent re

98 We developed a fluorescence anisotropy-based assay for the binding of S

99 rrent article describes the development of a fluorescence anisotropy-based assay that mimics the prin

100 We previously described a fluorescence anisotropy-based assay to measure these rat

101 Using fluorescence anisotropy-based binding analysis and recom

102 We implemented a homogeneous fluorescence anisotropy-based binding assay in an automa

103 ations of electrophoretic mobility shift and fluorescence anisotropy-based binding assays, we show th

104 Fluorescence anisotropy-based DNA-binding analysis demon

105 Using a fluorescence anisotropy-based DNA-binding assay, we exam

106 roles of carboxylates were also observed in fluorescence anisotropy-based ligand-binding assays.

107 We report a simple, rapid, and reproducible fluorescence anisotropy-based method for measuring rate

108 Such fluorescence anisotropy-based readout of chromatin compa

109 Quantitative DNA binding studies with fluorescence anisotropy-based titrations revealed that M

110 Fluorescence anisotropy binding and gel shift assays sho

111 Fitting these models to our fluorescence anisotropy binding data revealed that, surp

112 Fluorescence anisotropy binding measurements reveal that

113 s; combined with in vivo activity assays and fluorescence anisotropy binding measurements, these have

114 f HIF-1alpha, FGF-9, and p53 mRNAs and using fluorescence anisotropy binding studies, luciferase repo

115 t assays, fluorescence intensity changes and fluorescence anisotropy binding titrations.

116 ese two mechanisms, we used a combination of fluorescence anisotropy, biolayer interferometry, and do

117 Here we show that measurements of fluorescence anisotropy can be used to determine the hyd

118 Consequently, ThT fluorescence anisotropy cannot be directly used to study

119 sed of 200 nM APTS-MT and 1 microM ConA, the fluorescence anisotropy capably tracks the concentration

120 The key factors involved in the fluorescence anisotropy change were considered through t

121 Using fluorescence anisotropy competition assays it is shown t

122 Fluorescence anisotropy competition binding experiments

123 To this end, a fluorescence anisotropy competitive immunoassay for onli

124 Fluorescence anisotropy correlations, fluorescent lifeti

125 We show how fluorescence anisotropy could be used as a method to enc

126 configurations, and experimentally collected fluorescence anisotropy data displayed the predicted tre

127 ulated from MD simulations with experimental fluorescence anisotropy data showed excellent agreement,

128 Absorbance, fluorescence, and fluorescence anisotropy data were collected concurrently

129 Our hydrodynamic studies using fluorescence anisotropy decay and analytical ultracentri

130 edge are novel assays based on time-resolved fluorescence anisotropy decay and dynamic quenching meas

131 Nanosecond fluorescence anisotropy decay and picosecond fluorescenc

132 The fluorescence anisotropy decay for all compounds in miner

133 Herein, we report the use of fluorescence anisotropy decay for measuring the rotation

134 Time-dependent fluorescence anisotropy decay measurements confirm that,

135 Fluorescence anisotropy decay measurements show that tig

136 Fluorescence anisotropy decay of fluorescently labeled N

137 ons where simulations accurately capture the fluorescence anisotropy decay, we find at most a modest,

138 ined from measurements of diphenylhexatriene fluorescence anisotropy decay.

139 Fluorescence anisotropy decays were employed to probe th

140 cy calculated from the fit of the eGFP15eGFP fluorescence anisotropy decays with a stretched exponent

141 In this report, we demonstrate using a fluorescence anisotropy DNA-binding assay that the previ

142 tems where the fluorescence intensity and/or fluorescence anisotropy do not change upon interaction o

143 Changes were measured by fluorescence anisotropy, electron paramagnetic resonance

144 he certified values like absorption spectra, fluorescence anisotropy, excitation wavelength, and temp

145 Fluorescence anisotropy experiments at room temperature

146 Isothermal titration calorimetry and fluorescence anisotropy experiments corroborate these fi

147 gG Fc that is not conserved in IgA; however, fluorescence anisotropy experiments demonstrate that dir

148 Time-resolved fluorescence anisotropy experiments show that electronic

149 Results from fluorescence anisotropy experiments suggested that AFF4-

150 We utilized (i) time-resolved fluorescence anisotropy experiments to monitor the struc

151 absence of DNA in several assays, including fluorescence anisotropy experiments using a novel Alexa4

152 e modeled anisotropy decays to time-resolved fluorescence anisotropy experiments was obtained.

153 Addition of DNA to RAG1 and HMGB1 in fluorescence anisotropy experiments, however, results in

154 Based on fluorescence anisotropy experiments, ribosome toeprintin

155 ences with similar affinities as measured by fluorescence anisotropy experiments.

156 Here, we have used fluorescence anisotropy (FA) and a panel of NCS-1 EF-han

157 Using competitive fluorescence anisotropy (FA) and electrophoretic gel mob

158 Herein, we design novel fluorescence anisotropy (FA) aptamer sensing platforms d

159 A direct and competitive fluorescence anisotropy (FA) assay to probe both the met

160 A fluorescence anisotropy (FA) competition-based Shc Src h

161 In standard steady-state fluorescence anisotropy (FA) DNA-based assays, the ligan

162 y optimized encoded sensors for quantitative fluorescence anisotropy (FA) measurements of protein hyd

163 splay a protein binding-induced reduction of fluorescence anisotropy (FA), which is exclusively diffe

164 Structural studies using fluorescence anisotropy, fluorescence correlation spectr

165 by simultaneous readout of their brightness, fluorescence anisotropy, fluorescence lifetime, and emis

166 t fluorescence spectroscopic methods such as fluorescence anisotropy, fluorescence lifetimes and fluo

167 ucted a combined measurement of stopped-flow fluorescence anisotropy, fluorescence resonance energy t

168 Fluorescence anisotropy (fluorophore-tagged analogue exc

169 imentation boundary and by the relaxation of fluorescence anisotropy following rapid dilution of labe

170 actions were characterized via bead binding, fluorescence anisotropy, gel shift, and analytical ultra

171 Fluorescence anisotropy has been used to monitor the eff

172 Fluorescence anisotropy imaging and photobleaching exper

173 n of biomolecular interactions and establish fluorescence anisotropy imaging as a quantitative techni

174 Here we advance time-resolved fluorescence anisotropy imaging combined with two-photon

175 Using two-photon fluorescence anisotropy imaging of actin-GFP, we have de

176 We used fluorescence anisotropy imaging of histone H2B-EGFP to i

177 Our fluorescence anisotropy imaging provides an efficient wa

178 ignaling intermediates using high-resolution fluorescence anisotropy imaging.

179 d methods, fluorescence lifetime imaging and fluorescence anisotropy imaging.

180 labeled aptamers to SSB governed a very high fluorescence anisotropy increase (in the 0.130-0.200 ran

181 Time-resolved fluorescence anisotropy is a popular tool to study homo-

182 Fluorescence anisotropy is also highly sensitive to depo

183 Results from fluorescence anisotropy, isothermal titration calorimetr

184 The potential of laser-induced fluorescence anisotropy (LIFA) with CE to characterize i

185 xperimental approaches, including gel shift, fluorescence anisotropy, light scattering, and fluoresce

186 closan in vitro on the basis of steady-state fluorescence anisotropy, light scattering, and generaliz

187 atures and analyzed the membrane fluidity by fluorescence anisotropy measurement.

188 human LysRS using affinity pull-down assays, fluorescence anisotropy measurements and gel chromatogra

189 Fluorescence anisotropy measurements and the crystal str

190 Fluorescence anisotropy measurements indicated that MinC

191 Fluorescence anisotropy measurements indicated that sept

192 In this report, a method to multiplex fluorescence anisotropy measurements is described using

193 Time-resolved fluorescence anisotropy measurements of probes covalentl

194 Fluorescence anisotropy measurements of reagents compart

195 bright internal label in microscopy, and for fluorescence anisotropy measurements of RNA dynamics.

196 array of fluorescence techniques, including fluorescence anisotropy measurements of TMA-DPH anchored

197 Analysis of the binding of DNA to p58C by fluorescence anisotropy measurements revealed a strong p

198 Steady-state and time-resolved fluorescence anisotropy measurements show that the motio

199 Finally, fluorescence anisotropy measurements showed that the S14

200 Steady-state fluorescence anisotropy measurements suggest that glycop

201 In this study, time-resolved fluorescence anisotropy measurements were employed to de

202 Time-resolved fluorescence anisotropy measurements were used to provid

203 Time-resolved fluorescence anisotropy measurements with the single-Trp

204 Fluorescence anisotropy measurements yielded an equilibr

205 T binding, tyrosine intrinsic fluorescence, fluorescence anisotropy measurements, and solid-state NM

206 all angle X-ray scattering and time-resolved fluorescence anisotropy measurements, supports a sequent

207 nce recovery after photobleaching (FRAP) and fluorescence anisotropy measurements, that formation of

208 single tryptophan variants and time-resolved fluorescence anisotropy measurements, we determined that

209 ing constants in solution were obtained from fluorescence anisotropy measurements.

210 ydrolase have been detected by time-resolved fluorescence anisotropy measurements.

211 tion in the latter vesicles was confirmed by fluorescence anisotropy measurements.

212 Steady-state and time-resolved fluorescence anisotropy methods applied to an extrinsic

213 Analytical ultracentrifugation and fluorescence anisotropy methods show that the functional

214 n studied in solution using a combination of fluorescence anisotropy, microcalorimetry, and CD titrat

215 describe a strategy using a high throughput fluorescence anisotropy microplate assay to identify sma

216 a new application of our recently described fluorescence anisotropy microplate assay to investigate

217 col for simultaneous dual-channel two-photon fluorescence anisotropy microscopy acquisition to perfor

218 Here, we present a multiphoton fluorescence anisotropy microscopy live cell imaging tec

219 Furthermore, fluorescence anisotropy nanodisc assays revealed a direc

220 lin using analytical ultracentrifugation and fluorescence anisotropy, observing tubulin in virtually

221 Steady-state fluorescence anisotropies of intermediates indicate that

222 od to image simultaneously the positions and fluorescence anisotropies of large numbers of single mol

223 Time-resolved fluorescence anisotropy of 1 in cells confirms insignifi

224 TG2 activity by following an increase in the fluorescence anisotropy of a fluorescein-labeled substra

225 ith free tryptophan, as well as the decay of fluorescence anisotropy of a labeled protein.

226 raction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane ster

227 in vitro detection of organoarsenicals using fluorescence anisotropy of ArsR-DNA interactions.

228 One-photon, time-resolved fluorescence anisotropy of Bdp-Chol decays as a triexpon

229 ined when homo-FRET is measured by decreased fluorescence anisotropy of DiI-C16.

230 der, which is derived from the time-resolved fluorescence anisotropy of DPH.

231 ith its realization based on a dependence of fluorescence anisotropy of dye molecules on heat emissio

232 No change in fluorescence anisotropy of either TMA-DPH or DPH was obs

233 based on fluorescence lifetime of LHCII and fluorescence anisotropy of erythrosine shows a high rate

234 fibrils measured by dye-release experiments, fluorescence anisotropy of labeled lipid, and confocal a

235 ein S specifically and saturably altered the fluorescence anisotropy of PC/PS-bound active site-label

236 Dynamics were assayed by fluorescence anisotropy of the fluorescent base analogue

237 The fluorescence anisotropy of the polypeptide upon CaM titr

238 e fluidity of the lipid bilayer expressed as fluorescence anisotropy of the probe N,N,N-Trimethyl-4-(

239 By tracking the fluorescence anisotropy of this ligand, the ranges of Co

240 Here we report that the fluorescence anisotropy of YFP 10C depends on protein co

241 ccurs within 200 fs, resulting in a negative fluorescence anisotropy on excitation at 742 nm.

242 aracterization of hydrodynamic properties by fluorescence anisotropy or analytical ultracentrifugatio

243 We have developed a fluorescence anisotropy peptide probe using a geneticall

244 These results also demonstrate that a fluorescence anisotropy probe incorporated into a specif

245 rrelates well with the analysis based on the fluorescence anisotropy profile.

246 Fluorescence anisotropy results on variants of eotaxin-1

247 retention assays and binding measurement by fluorescence anisotropy reveal a heretofore unknown pref

248 Time-resolved fluorescence anisotropy revealed a significant decrease

249 NMR and fluorescence anisotropy revealed that Nterm binding to h

250 Fluorescence anisotropy reveals greater than 200-fold hi

251 Conversely, quantitative fluorescence anisotropy RNA binding assays and isotherma

252 to quantitatively interpret the increase of fluorescence anisotropy seen after photobleaching the ca

253 Using fluorescence anisotropy, SHAPE (selective 2'-hydroxyl ac

254 Stopped-flow FRET and fluorescence anisotropy show that complementary RNAs tra

255 Further experiments using fluorescence anisotropy showed a 10-fold reduction in RN

256 Although, fluorescence anisotropy showed that A3G had similar nano

257 his sensing platform allowed generation of a fluorescence anisotropy signal for aptamer probes which

258 n counting histogram analysis, time-resolved fluorescence anisotropy, single-molecule tracking, and s

259 ar dichroism, fluorescence and time-resolved fluorescence anisotropy spectroscopy.

260 Likewise, fluorescence anisotropy studies and binding studies with

261 Fluorescence anisotropy studies demonstrate that BOFP bi

262 ter interface monolayer surface pressure and fluorescence anisotropy studies reveal that the membrane

263 Fluorescence anisotropy studies showed that loss of CIP4

264 ding affinity of 3.6 microM as determined by fluorescence anisotropy studies.

265 This study was designed to use fluorescence anisotropy techniques to acquire steady-sta

266 olarization is associated with a decrease in fluorescence anisotropy that can be exploited to calcula

267 homo-FRET via a combination of time-resolved fluorescence anisotropy, the stretched exponential decay

268 been performed using fluorescence intensity, fluorescence anisotropy titration, and fluorescence reso

269 ethylated versus non-methylated sequences by fluorescence anisotropy titration.

270 Fluorescence anisotropy titrations reveal that KF binds

271 Fluorescence anisotropy titrations utilizing tC as a rep

272 us p53 DNA-binding sequences using automated fluorescence anisotropy titrations.

273 Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evalu

274 nd its consequent energy migration cause the fluorescence anisotropy to decrease as the number of lik

275 We used chemical dimerizers and fluorescence anisotropy to generate and visualize specif

276 Once trapped, we recorded the fluorescence anisotropy to investigate the diversity of

277 ng polymers, we took advantage of this large fluorescence anisotropy to make polarization-sensitive n

278 Using fluorescence anisotropy to obtain quantitative data, we

279 ircular dichroism, fluorescein emission, and fluorescence anisotropy to study the interaction between

280 Here, we used NMR and fluorescence anisotropy to study the interaction between

281 ve reconstituted the human 43 S PIC and used fluorescence anisotropy to systematically measure the af

282 (such as FRET, correlation spectroscopy, and fluorescence anisotropy) to monitor TRPV1 aggregation st

283 Using fluorescence anisotropy, TonB(33-239) was found to bind

284 Time-resolved fluorescence anisotropy (TRFA) has a rich history in eva

285 (1)H-DOSY NMR spectroscopy and time-resolved fluorescence anisotropy (TRFA) measurements.

286 VH)(t)) polarized emission for time-resolved fluorescence anisotropy (TRFA) measurements.

287 energy transfer (trFRET), and time-resolved fluorescence anisotropy (trFLAN) have been used to direc

288 hat, unlike small sized rigid molecules, the fluorescence anisotropy value of the free ThT in aqueous

289 he critical micelle concentration (CMC), the fluorescence anisotropy was independent of detergent con

290 Steady-state fluorescence anisotropy was used to examine the variatio

291 Fluorescence anisotropy was used to study the protein-pr

292 Binding to oligonucleotides, examined by fluorescence anisotropy, was positively cooperative and

293 Using fluorescence anisotropy, we determined a dissociation co

294 ce emission spectrum of ECD2 polypeptide and fluorescence anisotropy, we have demonstrated that this

295 ng electrophoretic mobility shift assays and fluorescence anisotropy, we report that CPSF30 selective

296 Here, using fluorescence anisotropy, we report that TRIP8b binding t

297 Using semi-native gels and fluorescence anisotropy, we show that Hfq undergoes a co

298 s and their intended targets, measured using fluorescence anisotropy, were also highly correlated wit

299 And we report for the first time the use of fluorescence anisotropy with intact human topoisomerase

300 nalysis combining sedimentation velocity and fluorescence anisotropy yielded Kd = 84 (54-123) nm Dime