ライフサイエンスコーパス: fluorescence polarization

1 protease cleavage reaction was monitored by fluorescence polarization.

2 demonstrated by oligonucleotide pulldown and fluorescence polarization.

3 s, which are then retested and quantified by fluorescence polarization.

4 e release of tracer results in a decrease in fluorescence polarization.

5 king of early binding events in real time by fluorescence polarization.

6 l assay for RecA-DNA filament assembly using fluorescence polarization.

7 ) for all 520 PDZ-peptide combinations using fluorescence polarization.

8 600 nM was measured by intrinsic tryptophan fluorescence polarization.

9 he RmFixL-RmFixJ complex was investigated by fluorescence polarization.

10 rates with membrane fluidity as measured by fluorescence polarization.

11 nt peptide 1 and the alanine analogues using fluorescence polarization.

12 es for compounds which significantly reduced fluorescence polarization.

13 haracterization of binding by measurement of fluorescence polarization.

14 in was realized by measuring the decrease in fluorescence polarization.

15 composed of NWs and quantum dots showed good fluorescence polarization.

16 le experimental setup and in real time using fluorescence polarization.

17 eractions with surface plasmon resonance and fluorescence polarization.

18 and SNAP-25B at the membrane, as measured by fluorescence polarization.

19 en DNA and the NPC fibrils was observed with fluorescence polarization.

20 Using a fluorescence polarization 30S-binding assay and a new cr

21 Here, we use a fluorescence polarization activity-based protein profili

22 With this goal in mind, we performed a fluorescence polarization activity-based protein profili

23 Here, we describe the development of a fluorescence polarization activity-based protein profili

24 We recently reported a fluorescence polarization-activity-based protein profili

25 We show that PME-1 is assayable by fluorescence polarization-activity-based protein profili

26 This change in fluorescence polarization allows an assay, termed DARET

27 Detection by fluorescence polarization allows real-time measurement o

28 Fluorescence polarization analysis indicated that the vI

29 Affinity was quantified by fluorescence polarization analysis of a fluorescein-tagg

30 Fluorescence polarization analysis revealed that the wil

31 polymer (MIP) synthetic antibody mimic, and fluorescence polarization analysis, for the direct detec

32 By screening 2500 fragments using fluorescence polarization and a thermal shift assay and

33 Fluorescence polarization and active site labeling, with

34 In addition, fluorescence polarization and binding assays suggest tha

35 Fluorescence polarization and competitive enzyme-linked

36 Using fluorescence polarization and electrophoretic mobility s

37 Fluorescence polarization and enzyme-linked immunosorben

38 ng affinity to biotin-4-fluorescein (B4F) by fluorescence polarization and intensity measurements.

39 duce an immediately-detectable shift in both fluorescence polarization and intensity.

40 Here, we describe fluorescence polarization and isothermal titration calor

41 ns and site-directed mutagenesis, along with fluorescence polarization and luciferase-refolding assay

42 ghly restricted, in agreement with data from fluorescence polarization and NMR relaxation studies.

43 Here we use analytical ultracentrifugation, fluorescence polarization and NMR-based enzymatic assays

44 Fluorescence polarization and solid-state assays indicat

45 or high-throughput screening assays based on fluorescence polarization and strategies for assay devel

46 Fluorescence polarization and surface plasmon resonance

47 DNA-binding site preferences in vitro using fluorescence polarization and thermal denaturation profi

48 Fluorescence polarization and this model were used to ch

49 Biophysical measurements using fluorescence polarization and two-dimensional NMR implic

50 a quantitative E2-E3 binding assay based on fluorescence polarization and used this assay to measure

51 Potency of ligands was determined by fluorescence polarization and/or isothermal titration ca

52 d biochemically through affinity pull downs, fluorescence polarization, and histone reader specificit

53 ide ligands using surface plasmon resonance, fluorescence polarization, and NMR and CD spectroscopic

54 Here, we use circular dichroism, fluorescence polarization, and NMR to demonstrate the pr

55 Using a combination of fluorescence polarization- and co-immunoprecipitation-ba

56 a simple relationship between the actin-GFP fluorescence polarization anisotropy and the actin polym

57 Here, we introduce a powerful fluorescence polarization anisotropy approach that utili

58 fluorescence lifetime imaging microscopy and fluorescence polarization anisotropy imaging, with compa

59 Fluorescence resonance energy transfer and fluorescence polarization anisotropy studies revealed th

60 Fluorescence polarization anisotropy was used for solid-

61 he p40 subunit of the Arp2/3 complex and, by fluorescence polarization anisotropy, the binding of act

62 tration (CMC) of various detergents based on fluorescence polarization (anisotropy) of the lipophilic

63 first real-time RNase P activity assay using fluorescence polarization/anisotropy (FP/FA) with a 5' e

64 Here, we describe a new fluorescence polarization aptamer assay (FPAA) strategy

65 t since fluorescent techniques like FRET and fluorescence polarization are now commonly used in prote

66 rent cELISA, an indirect ELISA (iELISA), and fluorescence polarization assay (FPA).

67 in and extracts of cultured keratinocytes by fluorescence polarization assay against fluorogenic subs

68 Fluorescence polarization assay and molecular modeling s

69 low nanomolar binding affinity for FimH in a fluorescence polarization assay and submicromolar cellul

70 Here we report the development of a fluorescence polarization assay based on the binding to

71 nhibitors, we have developed and optimized a fluorescence polarization assay based on the interaction

72 Using a fluorescence polarization assay designed to screen for i

73 d sarcosine scans coupled with a competitive fluorescence polarization assay developed for identifyin

74 led Forster resonance energy transfer (FRET)-fluorescence polarization assay for botulinum neurotoxin

75 evelopment of a homogeneous, high-throughput fluorescence polarization assay for identifying compound

76 The probe was successfully used to develop a fluorescence polarization assay for these six AAAEs, and

77 The fluorescence polarization assay holds promise for the di

78 An in vitro fluorescence polarization assay identified point mutatio

79 development of a competitive high-throughput fluorescence polarization assay in a 384-well format to

80 Fluorescence polarization assay reveals that BXI-61 and

81 A fluorescence polarization assay showed that LL-37 was ab

82 A fluorescence polarization assay shows that Hsp90 (MEEVD

83 rmed the experiments using a non-radioactive fluorescence polarization assay that quantifies the degr

84 To assist this effort, we have devised a fluorescence polarization assay that quantifies the inte

85 Rgg/SHP interactions were determined using a fluorescence polarization assay that utilized FITC-label

86 icroplate-based fluorescence anisotropy (FA)/fluorescence polarization assay that works well, even wi

87 scribe the development and optimization of a fluorescence polarization assay to identify potential in

88 systematic development and optimization of a fluorescence polarization assay to identify small molecu

89 an initial hit compound, we have developed a fluorescence polarization assay to monitor binding of co

90 confirm this hypothesis by using a sensitive fluorescence polarization assay to show that both WW dom

91 We have developed a high-throughput fluorescence polarization assay utilizing a fluorescein-

92 NC-viral RNA/DNA binding, a high-throughput fluorescence polarization assay was developed and a libr

93 pes involved in CARP-1 binding with APC-2, a fluorescence polarization assay was established that ind

94 The fluorescence polarization assay was then miniaturized to

95 f nuclear magnetic resonance binding assays, fluorescence polarization assay, and computational docki

96 Using a fluorescence polarization assay, we show a high conserva

97 zation was the development of a new covalent fluorescence polarization assay, which allows for the di

98 Fluorescence polarization assay-based high throughput sc

99 (LY5), was confirmed to bind to STAT3 SH2 by fluorescence polarization assay.

100 hylcytosine (glu-5-hmC) using an equilibrium fluorescence polarization assay.

101 0 of 125 nM versus 290 nM for peptide 1 in a fluorescence polarization assay.

102 ands using a newly developed high-throughput fluorescence polarization assay.

103 EEI (IC(50) = 6.5 microM), as evaluated by a fluorescence polarization assay.

104 d their K(D)'s for SLF were measured using a fluorescence polarization assay.

105 were evaluated for MDM2-p53 inhibition in a fluorescence polarization assay.

106 real-time peptide association kinetics using fluorescence polarization assays and comparing the exper

107 d N-terminal APN region using direct binding fluorescence polarization assays and thermodynamic analy

108 ion, glutathione S-transferase pulldown, and fluorescence polarization assays employing synthetic BH3

109 Fluorescence polarization assays showed that a number of

110 Gel-shift and fluorescence polarization assays showed that each aptame

111 Fluorescence polarization assays were carried out to eva

112 bodies, including ELISAs, Western blots, and fluorescence polarization assays, are complex, multiple-

113 Together with sedimentation velocity and fluorescence polarization assays, the crystal structure

114 In fluorescence polarization assays, we show that BCL11A co

115 ethylstat and its application as a tracer in fluorescence polarization assays.

116 g experiments such as radioligand assays and fluorescence polarization assays.

117 easurement techniques, such as microarray or fluorescence polarization assays.

118 ased the fluorescence intensity and level of fluorescence polarization at an excitation wavelength of

119 specific interaction, we developed a direct fluorescence polarization based method for the detection

120 An activity assay utilized fluorescence polarization, based on the binding of fluor

121 Here, we report a fluorescence polarization-based assay for human CypA tha

122 simple high-throughput screening compatible fluorescence polarization-based assay that can be used t

123 ed MIF inhibitors and their use in the first fluorescence polarization-based assay to measure the dir

124 mains, we also developed and validated a new fluorescence polarization-based assay.

125 In this work, we report a fluorescence polarization-based binding assay for determ

126 to that of the natural Smac peptide using a fluorescence polarization-based binding assay.

127 Fluorescence polarization-based experiments revealed tha

128 ed the utility of our SNP marker set and new fluorescence polarization-based genotyping methods for s

129 A protease-coupled fluorescence polarization-based high-throughput screen w

130 -ribose dimer was then utilized in a general fluorescence polarization-based PAR-protein binding assa

131 Synthesized probes also facilitated a fluorescence polarization-based screen for dGMII inhibit

132 In this effort, fluorescence polarization-based screening was used to id

133 d pharmacophore-modeling-based in silico and fluorescence polarization-based screening we identified

134 Here, we report the development of a fluorescence polarization-based, high-throughput screeni

135 hits via fragment growing using a dedicated fluorescence-polarization-based assay as the structure-a

136 We report here a novel high-throughput fluorescence polarization binding assay and its applicat

137 Our fluorescence polarization binding assays show that ZF4 h

138 used to investigate this interaction through fluorescence polarization binding assays.

139 ion-free, homogeneous, and simple to perform fluorescence polarization bioassay for paclitaxel.

140 Data from fluorescence polarization, cell-cell fusion, and viral i

141 ln-NHBn (21), which had an IC(50) of 162 nM (fluorescence polarization), compared to 290 nM for the l

142 A fluorescence polarization competition assay has been dev

143 destabilizing domain was determined using a fluorescence polarization competition assay, and the GFP

144 n on ASGR binding, we tested and validated a fluorescence polarization competition binding assay.

145 Binding constants obtained from fluorescence polarization data for toxin A binding to to

146 From the fluorescence polarization data, it is estimated that one

147 t K(D) of 1.7 x 10(-6) m, as determined from fluorescence polarization data.

148 ed an overall decrease in diphenylhexatriene fluorescence polarization (decreased molecular order) in

149 Fluorescence polarization delineated lateral breast canc

150 Fluorescence polarization detected binding between Nox4

151 cted dye-terminator incorporation assay with fluorescence polarization detection was used as a high-t

152 However, fluorescence polarization did reveal non-sequence-specif

153 tified via high-throughput screening using a fluorescence polarization displacement assay.

154 of the rBH3 peptides using NMR spectroscopy, fluorescence polarization displacement assays, and alani

155 magnetic resonance spectroscopy techniques, fluorescence polarization displacement assays, and cell-

156 Fluorescence polarization experiments establish that TAP

157 Using fluorescence polarization experiments, we demonstrate th

158 ptides that is the electrochemical analog of fluorescence polarization (fluorescence anisotropy), a v

159 a homogeneous high-throughput assay based on fluorescence polarization for measuring the binding affi

160 tes the use of a smartphone-based sensor for fluorescence polarization (FP) analysis of biomolecules.

161 l affinity for phosphochemicals (IMAP)-based fluorescence polarization (FP) and time-resolved fluores

162 have developed and optimized a competitive, fluorescence polarization (FP) assay for accurate and ra

163 In this study, we developed a protein-RNA fluorescence polarization (FP) assay for high-throughput

164 We report the development of a fluorescence polarization (FP) assay for PBPs and "serin

165 An automated fluorescence polarization (FP) assay has been developed

166 dy, we describe the development of a kinetic fluorescence polarization (FP) assay that enables measur

167 Here we describe the development of a fluorescence polarization (FP) assay that is amenable fo

168 a competitive, homogeneous, high-throughput fluorescence polarization (FP) assay to identify small m

169 Here we report the development of a fluorescence polarization (FP) assay using an engineered

170 A fluorescence polarization (FP) assay was developed to de

171 A fluorescence polarization (FP) assay which monitors the

172 for fragment finding, and thermal shift and fluorescence polarization (FP) assays are used to a less

173 well format and 3-mul final volume a pair of fluorescence polarization (FP) assays using fluorescein-

174 We developed a fluorescence polarization (FP) based assay and measured

175 hods were developed, including a homogeneous fluorescence polarization (FP) competition assay that re

176 Initial studies, employing a fluorescence polarization (FP) competition assay, reveal

177 IC50 = 50-100 nM) for Hsp90 as measured in a fluorescence polarization (FP) competitive binding assay

178 ing a primer extension genotyping assay with fluorescence polarization (FP) detection.

179 nal distributions and dynamics obtained from fluorescence polarization (FP) experiments on ordered sy

180 say for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput scree

181 s model enzymes to evaluate the potential of fluorescence polarization (FP) for high-throughput scree

182 A homogeneous fluorescence polarization (FP) ligand binding assay capa

183 A fluorescence polarization (FP) microplate assay suitable

184 binding- and displacement-induced changes in fluorescence polarization (FP) of fluorescent small mole

185 to target DNAs is followed by measuring the fluorescence polarization (FP) of the dyes.

186 Fluorescence polarization (FP) provides a nondisruptive

187 his approach, which is based on steady-state fluorescence polarization (FP) spectroscopy, kinetically

188 Here, we report a novel fluorescence polarization (FP) technique for examining a

189 developed a high-throughput assay that uses fluorescence polarization (FP) to distinguish the fluore

190 Here we report the use of fluorescence polarization (FP) to measure the Kd for the

191 After comparing fluorescence polarization (FP), homogeneous time-resolve

192 biochemical and biophysical methods, such as fluorescence polarization (FP), isothermal titration cal

193 Using fluorescence polarization (FP), we have demonstrated tha

194 K(d)) of the two viral proteins, measured by fluorescence polarization (FP), were similar, and both N

195 In this study, we developed a fluorescence polarization (FP)-based BCL9 binding assay.

196 A fluorescence polarization (FP)-based binding assay was c

197 ve detection of immunoglobulin E (IgE) using fluorescence polarization (FP).

198 affinity capillary electrophoresis (ACE) and fluorescence polarization (FP).

199 Fluorescence polarization(FP) and time resolved fluoresc

200 We discovered that the fluorescence polarization (Fpol) of Methylene Blue (MB)

201 anated fibers from rabbit psoas muscle using fluorescence polarization from bifunctional rhodamine pr

202 ient in a muscle fiber allows observation of fluorescence polarization from C-term GFP-tagged HCRLC e

203 Fluorescence polarization, giving probe angle and its di

204 3 compounds using a human PKD1 (PKCmu)-based fluorescence polarization high throughput screening assa

205 ultiple methods, including pull-down assays, fluorescence polarization, hydrogen/deuterium exchange,

206 potency than that of the lead, as judged by fluorescence polarization (IC(50) values were 110 and 13

207 Wide-field fluorescence polarization images were excited at 640 nm

208 Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are complementary tech

209 However, OCT complemented fluorescence polarization imaging by facilitating cross-

210 We confirmed that fluorescence polarization imaging did not affect the via

211 rgins using combined dye-enhanced wide-field fluorescence polarization imaging for en face cancer mar

212 Combined PS OCT and fluorescence polarization imaging shows promise for intr

213 s and the results are in good agreement with fluorescence polarization immunoassay (FPIA) and LC-MS/M

214 ared our assay in parallel with a commercial fluorescence polarization immunoassay (FPIA) kit in dete

215 g detection with that in the clinically used fluorescence polarization immunoassay (FPIA).

216 A high-throughput, competitive fluorescence polarization immunoassay has been developed

217 correlate well with the values determined by fluorescence polarization immunoassay.

218 This observation implies a new generation of fluorescence polarization immunoassays with broad applic

219 ocedures, suggesting that the application of fluorescence polarization in combination with CypA is hi

220 ocedures, suggesting that the application of fluorescence polarization in combination with recombinan

221 In addition, the intrinsic fluorescence polarization increases from 0.327 +/- 0.001

222 on with OmcA, there was a marked increase in fluorescence polarization indicating the formation of a

223 Fluorescence polarization is an effective bridge between

224 s kinetic rotating droplet electrochemistry, fluorescence polarization, isothermal titration calorime

225 We used a commercially available fluorescence polarization ligand binding assay to invest

226 We utilized fluorescence polarization measurements and hydrogen-deut

227 cein-labeled digoxigenin obtained by FCS and fluorescence polarization measurements are identical.

228 Fluorescence polarization measurements demonstrated moti

229 NMR and fluorescence polarization measurements showed that the C

230 Fluorescence polarization measurements then allow the di

231 Applying single-molecule fluorescence polarization measurements to characterize t

232 interaction is below the detection limit of fluorescence polarization measurements, but chemical cro

233 n spectroscopy together with single molecule fluorescence polarization measurements, we have determin

234 The developed fluorescence polarization method screens for small molec

235 on constant between ST1 and ST1710 using the fluorescence polarization method.

236 complementary thioflavin T fluorescence and fluorescence polarization methods.

237 Here, by using a novel fluorescence polarization microscope that reports the po

238 We built an instantaneous fluorescence polarization microscope, which simultaneous

239 ue, here we use ensemble and single molecule fluorescence polarization microscopy (FPM) to determine

240 ed actin filament order in human cells using fluorescence polarization microscopy and found that clea

241 Herein, we present an excitation resolved fluorescence polarization microscopy approach to measure

242 nality, we used ensemble and single-molecule fluorescence polarization microscopy to analyze the beha

243 Our results demonstrate the ability of fluorescence polarization microscopy to measure amphipat

244 opy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magn

245 We employed fluorescence polarization microscopy to show that cadher

246 In this work, fluorescence polarization microscopy was applied to inve

247 within the nuclear pore complex (NPC) using fluorescence polarization microscopy.

248 integrin head, and measure orientation with fluorescence polarization microscopy.

249 Site-directed fluorescence polarization of 7-nitrobenz-2-oxa-1,3-diazo

250 y of the thioesterase domain, estimated from fluorescence polarization of a pyrenebutyl methylphospho

251 Statistically significant higher fluorescence polarization of cancer as compared with bot

252 We determined that higher fluorescence polarization of MB occurs due to its increa

253 Fluorescence polarization of membrane probes showed that

254 entation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensemb

255 as model compounds, we demonstrated that the fluorescence polarization of the peptide probe increased

256 hysical effects of indomethacin, we measured fluorescence polarization of the phase-sensitive probe L

257 ramatically and differentially increased the fluorescence polarization of the phosphorylated peptide

258 te chemoreceptor, monitored the steady-state fluorescence polarization of YFP, and found that the pol

259 cence lifetime imaging microscopy (FLIM) and fluorescence polarization or anisotropy imaging microsco

260 of protein microarrays with the fidelity of fluorescence polarization, our domain/peptide-based stra

261 n machinery in the HA stem, we synthesized a fluorescence-polarization probe based on a recently desc

262 ent was found between ELISA measurements and fluorescence polarization results.

263 Equilibrium DNA binding activity measured by fluorescence polarization reveals no difference in ssDNA

264 In a fluorescence polarization screen for the MYC-MAX interac

265 A high throughput fluorescence polarization screen that quantifies the inh

266 binatorial library synthesis and competitive fluorescence polarization screening approach that transf

267 and procedural framework for high-throughput fluorescence polarization screens to aid in this effort.

268 ncompetitive and competitive components, and fluorescence polarization shows directly that the inhibi

269 e oligonucleotide is sufficiently short, the fluorescence polarization signal can also be used to det

270 escein-labeled c-myc peptide produced a high-fluorescence polarization signal upon binding to the c-m

271 the presence of inhibitors by monitoring the fluorescence polarization signal.

272 ividual macromolecules using single molecule fluorescence polarization (SMFP) microscopy.

273 ell with results obtained by single molecule fluorescence polarization spectroscopy, indicating a lar

274 Initial in vitro fluorescence polarization studies confirmed that specifi

275 Fluorescence polarization studies indicated that apoCaM

276 Fluorescence polarization studies of small unilamellar v

277 ine residues, as assumed in previous in situ fluorescence polarization studies.

278 C1 peptides using crystallographic, NMR, and fluorescence polarization studies.

279 The changing of the fluorescence polarization suggests that the motions of t

280 Combining fluorescence polarization, super-resolution microscopy,

281 n of the FKBP.rapamycin.FRB complex, we used fluorescence polarization, surface plasmon resonance, an

282 The described fluorescence polarization technique allows very low (sub

283 in of brain myosin V using a single-molecule fluorescence polarization technique that determines the

284 y fluorescence resonance energy transfer and fluorescence polarization techniques.

285 orted Keap1-Nrf2 PPI inhibitor classes using fluorescence polarization, thermal shift assay, and surf

286 We further developed an assay based on fluorescence polarization to assess the mechanism of the

287 We used protein microarrays and quantitative fluorescence polarization to characterize the binding se

288 R spectroscopy, biolayer interferometry, and fluorescence polarization to characterize the Hsc70-CHIP

289 sensitive biochemical assay that relies upon fluorescence polarization to indicate peptide interactio

290 rgy transfer between identical fluorophores) fluorescence polarization to monitor dynamic changes in

291 This peptide had a six fold increase in fluorescence polarization upon binding to ZipA(185-328)

292 The change of fluorescence polarization upon duplex formation inversel

293 es, which reveal fluorescence enhancement or fluorescence polarization upon their binding to DNA and

294 These behaviors were confirmed by fluorescence polarization using TAMRA-labeled peptide.

295 d enzyme concentration-dependent decrease in fluorescence polarization was observed in the COMT assay

296 eled DNA, and following the changes in their fluorescence polarization, we found direct evidence that

297 eous high-throughput assays, AlphaScreen and fluorescence polarization, were established to study bet

298 Using fluorescence polarization, which reflects the mobility o

299 ted for tubulin binding, causing a change in fluorescence polarization, which was an inverse function

300 fied to remove excess primer, or by means of fluorescence polarization without any additional cleanup