ライフサイエンスコーパス: fluorescent label

1 a membrane/lipophilic probe and as a peptide fluorescent label.

2 cted by the presence and localization of the fluorescent label.

3 e-1,3,6-trisulfonic acid to add charge and a fluorescent label.

4 inding site to be gauged with respect to the fluorescent label.

5 was probed simultaneously with only a single fluorescent label.

6 stability make pyrene excimer an attractive fluorescent label.

7 proteins that carry two spectrally distinct fluorescent labels.

8 or secondary labeling with relatively bulky fluorescent labels.

9 ide substrates by attaching PEG moieties and fluorescent labels.

10 olic signatures without the use of exogenous fluorescent labels.

11 fluence depends sensitively on the charge of fluorescent labels.

12 tiplexed measurements can be performed using fluorescent labels.

13 abel-free monitored instead of using several fluorescent labels.

14 ular morphological changes and intracellular fluorescent labels.

15 r molecules, cells and tissues with multiple fluorescent labels.

16 r of complementary hairpin oligomers bearing fluorescent labels.

17 beled with up to four spectrally overlapping fluorescent labels.

18 is especially critical when detecting single fluorescent labels.

19 different reported sSMLM system designs and fluorescent labels.

20 ment with the flow cytometric analysis using fluorescent labels.

21 ains comprising a multitude of cell-specific fluorescent labels.

22 h high specificity that correlates well with fluorescent labels.

23 sphate and Ca(2+), or by the identity of the fluorescent labels.

24 eshly excised human skin using two different fluorescent labels.

25 nd in vivo without the use of any additional fluorescent labels.

26 ~100 events per second without the need for fluorescent labeling.

27 try and photophysics, or the requirement for fluorescent labeling.

28 ick reactivity of both congeners observed by fluorescent labeling.

29 d and visualized by click-chemistry-mediated fluorescent labeling.

30 we designed a DDR2 construct appropriate for fluorescent labeling.

31 l cord and peripheral nerve that requires no fluorescent labeling.

32 oprotein of interest for subsequent specific fluorescent labeling.

33 metry to study the degree and positioning of fluorescent labeling.

34 h of PC12 cells was analyzed using ELISA and fluorescent labeling.

35 al nanoparticles for magnetic extraction and fluorescent labeling.

36 n containing only HPDL cells as monitored by fluorescent labeling.

37 ynthetic peptides, followed by bioorthogonal fluorescent labeling.

38 V staining strategy, with the amine-reactive fluorescent label, 5-(and-6)-Carboxyfluorescein Diacetat

39 Fluorescent-labeled (68)Ga-tilmanocept allows for PET im

40 Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS

41 tages: use of unlabeled protein to eliminate fluorescent labeling, ability for characterization of pr

42 e velocities and displacements of individual fluorescent-labeled actin segments, at varying times thr

43 ducing end of disaccharides with an aromatic fluorescent label affords stable derivatives with proper

44 Finally, we found that site-specific fluorescent labeling allows monitoring of the transient

45 e-1,3,6-trisulfonic acid (APTS) to provide a fluorescent label and a triple-negative charge, separate

46 es, we show the successful introduction of a fluorescent label and biotin for detection or affinity e

47 uding, the delivery of reagents to cells for fluorescent labeling and cell-surface engineering, the f

48 synthetic system permits cell-type-specific fluorescent labeling and exogenous variation of the rela

49 Fluorescent labeling and immunostaining showed that VF n

50 ce poor settings by eliminating the need for fluorescent labeling and optical detection instrumentati

51 As a proof of concept, localized fluorescent labeling and pH changes were purposely intro

52 We first outline the fluorescent labeling and quantification of two common en

53 Fluorescent labeling and scanning electron microscopy (S

54 In vivo fluorescent labeling and subcellular fractionation revea

55 Using site-specific fluorescent labeling and super-resolution microscopy, we

56 after treatment ended was confirmed through fluorescent labeling and tracking.

57 mplex co-culture environments often requires fluorescent labelling and significant light exposure tha

58 age, an unrivaled choice of small and highly fluorescent labels and - thanks to recent advances in cl

59 used alternative to signal amplification via fluorescent labels and enzymatic methods.

60 nthetic and biophysical chemists develop new fluorescent labels and isomorphic nucleobases as well as

61 ribosomes must be specifically labeled with fluorescent labels and molecular handles.

62 The design of four new fluorinated biaryl fluorescent labels and their attachment to nucleosides a

63 enerate tumor-specific affinity ligands with fluorescent labels and/or azide modifications at high pu

64 uantitative study on the binding kinetics of fluorescent-labeled and un-labeled molecules (lectin pro

65 rcumvents the need for bisulfite conversion, fluorescent labeling, and PCR and could therefore prove

66 ry, which was confirmed by enzymatic assays, fluorescent labeling, and small-angle x-ray scattering.

67 oes not require surface functionalization or fluorescent labels, and has the potential to serve as a

68 To determine utility of a panel of six fluorescent labeled antibodies as a diagnostic tool for

69 We created a fluorescent-labeled antibody that recognizes periostin a

70 Fluorescent-labeled antigen experiments indicated that a

71 Fluorescent labels are commonly used in bioassays to enh

72 Fluorescent labels are widely employed in biomarker quan

73 Using a variety of intracellular fluorescent labels as well as negative staining experime

74 summarized, and the applications in redox or fluorescent labeling, as well as in bioconjugations and

75 Fluorescent labeling at position 14, 32, 49, or 85 did n

76 By comparison, fluorescent labeling at position 41 reduced the affinity

77 in high yield by hydrazinolysis, followed by fluorescent labeling at the reducing terminus and high-p

78 Dual fluorescent labels attached to sandwich ssDNA probes wer

79 The method, applied to fluorescent labels attached to single actin filaments, p

80 have labeled the Cu-protein azurin with the fluorescent label ATTO 655-N-hydroxysuccinimide(NHS)-est

81 also tested on RAW macrophages by the use of fluorescent-labeled bacteria.

82 We report a novel class of biofunctional fluorescent labels based on trapping of approximately 10

83 eal-time imaging in zebrafish displayed that fluorescent-labeled blood vessels showed enhanced intrat

84 We used parabiosis to introduce fluorescent-labeled bone marrow-derived cells to mice wi

85 tylcholine receptor-stained NMJs detected by fluorescent labelling, but following salbutamol treatmen

86 ed by SIM, followed by imaging of one of the fluorescent labels by dSTORM.

87 31,000 and Fungal 60,000) preparations using fluorescent-labelled casein, meat myofibrillar and conne

88 LSC populations were identified using fluorescent-labeled cell sorting and transplantation int

89 of the online FACS-Chip-LCMS workflow, 5000 fluorescent labeled cells were enriched from a 5% hetero

90 Studies with fluorescent-labeled cells revealed that the melanoma cel

91 acted with glutathione-S-transferase and the fluorescent labeling compound monochlorobimane to form a

92 Using fluorescent labeling, confocal microscopy, and 3D recons

93 hod for sequencing DNA that does not require fluorescent labelling could reduce costs and increase se

94 By employing fluorescent-labeled cysteine mutations, we observe that

95 oE3 and apoE4 also inhibited the uptake of a fluorescent-labeled derivative of the peptide, suggestin

96 nsemble and single molecule assays employing fluorescent labeled derivatives of EF-Tu, tRNA, and the

97 scherichia coli to directly visualize single fluorescent labeled DNA polymerase I (Pol) and ligase (L

98 We measured the distance between fluorescent-labeled DNA loci of various interloci contou

99 wever, care must be taken to ensure that the fluorescent labels do not influence the system being pro

100 3% and 2.4% of the examined gonads contained fluorescent labeled donor PGCs.

101 Fluorescent-labeled donor T cells were detected in CNS l

102 -Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye.

103 ectron cryotomography, mass spectrometry and fluorescent labelling dyes, here we show that some envir

104 pectrometry is a valuable tool for measuring fluorescent labeling efficiency and specificity.

105 Trichoderma reesei using the colocation of a fluorescent label (enhanced yellow fluorescence protein,

106 diolabeled drug quantified bulk delivery and fluorescent label established penetration of drug over f

107 protein synthesis of modified (e.g. mutated, fluorescent-labeled) exogenous components (ribosomes, am

108 Image analysis of fluorescent labeled exosomes derived from three cell lin

109 Uptake profile of fluorescent-labelled exosomes in epithelial cells was as

110 porium OB3b was demonstrated by isotopic and fluorescent labeling experiments.

111 icroscopic phase separation is detectable by fluorescent labeling, followed by cooling of the membran

112 quantum dots (QDs) and their application as fluorescent label for immunoassay.

113 aine rotaxane fluorophore, SeTau-647, as the fluorescent label for insulin.

114 on-enhanced spin sensors as well as improved fluorescent labeling for bioimaging could be envisioned.

115 Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indire

116 able fluorophores, a shortage of photostable fluorescent labels for bacteria has limited its use in t

117 Such methods, however, usually rely on fluorescent labels for chemical targeting, which could p

118 The specifics of quantum dots use as fluorescent labels for continuous monitoring under const

119 ve alternative to existing nanoparticles and fluorescent labels for non-invasive targeted imaging of

120 l serve as a valuable reference for choosing fluorescent labels for single-molecule motility assays.

121 les multiplexing by incorporating orthogonal fluorescent labels for the simultaneous detection of dif

122 While methods that use fluorescent labels for visualizing printed arrays prior

123 proteins into chloroplasts, and that provide fluorescent labels for visualizing the resultant structu

124 Retrograde fluorescent labeling from the site of transection combin

125 Fluorescent labeling from viral replication is thereby r

126 By microscopic analysis of fluorescent-labeled GalR, a regulon-specific transcripti

127 The study shows that fluorescent labeling has a significant influence on the

128 arch, and in particular the use of molecular fluorescent labels, has allowed investigation of heterog

129 Two-fold increase in accumulation of fluorescent labelled high-binding hFcRn albumin, compare

130 filaments by determining the orientation of fluorescent labels, however with a strong drawback: pola

131 The majority of this review will focus on fluorescent labeling; however, the principles can often

132 for applications where samples contain many fluorescent labels; however, existing methods perform po

133 Enhanced photostability of fluorescent labels (i.e., maximum emitted photons before

134 ation in 96-well plates, glycan release, and fluorescent labeling; (ii) quantitative HPLC analysis, i

135 4/80(+) cells) were characterized by in vivo fluorescent labeling, immunohistochemistry, fluorescence

136 beled, and eluted separately from the excess fluorescent label in 3D printed microfluidic systems.

137 and excellent stability to function as a NIR fluorescent label in the early detection of tumors.

138 hydrogel degradation products containing the fluorescent label in the surrounding tissues revealed a

139 ght exposure), a very promising property for fluorescent labeling in biology.

140 Our work indicates that fluorescent labeling in general affects the binding beha

141 bility to visualize macrophage cells without fluorescent labeling in the live human eye represents an

142 notags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, bec

143 oteins, specifically Smoothened, with single fluorescent labels in fixed cells, we use three-dimensio

144 ly on a powerful direct binding assay called fluorescent labels in kinases ('FLiK'), which enables a

145 ynapses in mature transgenic mice expressing fluorescent labels in neurons and microglia.

146 Varying the position of the fluorescent labels in the Htt monomer with expanded 51Q

147 nanoparticles are potentially interesting as fluorescent labels in, for instance (single particle), i

148 c substrate (water), and without introducing fluorescent labels, in fact, without utilizing any elect

149 more proteins than previously possible with fluorescent labels, including surface markers, cytokines

150 Pulse-chase fluorescent labeling indicates that Nup188 populates cen

151 caspase-1 was detected using FAM-YVAD-FMK, a fluorescent-labeled inhibitor of caspases (FLICA) specif

152 Incorporation of diffusible fluorescent labels into the viral membrane and the viral

153 eoxynucleotide 5'-tetraphosphates in which a fluorescent label is attached to the terminal phosphate

154 Fluorescent labeling is a mainstream technology for dete

155 e cell RNA imaging using genetically encoded fluorescent labels is an important tool for monitoring R

156 The precise determination of the position of fluorescent labels is essential for the quantitative stu

157 Bioimage analysis of fluorescent labels is widely used in the life sciences.

158 Ribosomes containing fluorescent-labeled L11 and tRNAs labeled with fluoropho

159 Here, we track single, fluorescent-labeled large DNA molecules (11, 115 kbp) di

160 Myocardial vascular density (fluorescent-labeled lectin perfusion and CD31 immunofluo

161 remity muscle with slice recordings from the fluorescent-labeled lumbar MN cell bodies to establish t

162 owever, there is no electrical analogue to a fluorescent label, meaning that it is not possible to id

163 bination-mediated, noninvasive combinatorial fluorescent labeling method for embryonic lineage tracin

164 Here, we present a single RNA sensitive fluorescent labeling method which allows us to label and

165 bility to detect nitroaromatic analytes, the fluorescent-labeled MIP particles were tested for their

166 of this study, it can be concluded that the fluorescent-labeled MIP system is a feasible method for

167 e a co-localized brain-wide data set of both fluorescent-labelled neurons and counterstained cell bod

168 ted the basis for previous observations that fluorescent-labeled neuropeptide Y (NPY) is usually rele

169 neuronal calcium imaging, colocalization of fluorescent labels, non-invasive pH measurement, and oth

170 e used a model of intradermal injection with fluorescent-labeled, nonviable larvae from the helminth

171 Fluorescent-labeled NPs were used to show uptake into ma

172 The inherent assumption is that the fluorescent label observed under LM colocalises well wit

173 photon uncaging with two-photon imaging of a fluorescent label of surface AMPA receptors to monitor i

174 Post-treatment fluorescent labeling of 1 observed by confocal fluoresce

175 mportantly, combining our size reporter with fluorescent labeling of a different protein in a differe

176 Its use is demonstrated by the fluorescent labeling of active UCHL1 both in vitro and i

177 lving problems encountered in widely applied fluorescent labeling of biomolecules for studying life p

178 ed dienophiles have numerous applications in fluorescent labeling of biomolecules.

179 Multicolor fluorescent labeling of both intra- and extracellular st

180 Fluorescent labeling of budding yeast nucleoli with CDC1

181 campal CA1 neurons using genetic methods for fluorescent labeling of dendritic spines.

182 Retrograde fluorescent labeling of dental primary afferent neurons

183 We provide protocols to achieve fluorescent labeling of ESCs in ~2-3 weeks, which allows

184 ents was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shear

185 yR1 and RyR2 isoforms, we used site-directed fluorescent labeling of FKBP12.6, ligand binding measure

186 licability of CuAIAC was demonstrated by the fluorescent labeling of functionalized polystyrene and b

187 Post-treatment fluorescent labeling of functionalized Pt(II)-based agen

188 peracetylation by Gcn5, we used differential fluorescent labeling of histones to monitor acetylation

189 ated fluorescein isothiocyanate (FITC) after fluorescent labeling of human serum albumin (HSA) with e

190 s work enables the site-specific, live-cell, fluorescent labeling of intracellular proteins at high d

191 Kme3), CX4-ONBD, is used to direct selective fluorescent labeling of Kme3 histone peptides.

192 Fluorescent labeling of LIT HLN B cells before adoptive

193 roaches to assess cytosolic delivery rely on fluorescent labeling of macromolecules with an "always o

194 Furthermore, a spatially controlled fluorescent labeling of microtubules in live CHO cells w

195 Several methods exist for fluorescent labeling of N-glycans and subsequent chromat

196 Fluorescent labeling of pairs of sites on VHL for fluore

197 rporation Mediated by Enzymes), a method for fluorescent labeling of peptide-fused recombinant protei

198 sion of the biarsenical-tetracysteine tag to fluorescent labeling of peptides and proteins in chemica

199 alcitonin (sCT) demonstrates the utility for fluorescent labeling of polymers and proteins.

200 e as the green fluorescent protein, enabling fluorescent labeling of proteins in a reversible and spe

201 Site-specific fluorescent labeling of proteins inside live mammalian c

202 The first issue is fluorescent labeling of proteins.

203 say for measuring MshB activity requires the fluorescent labeling of reaction mixtures and subsequent

204 tech, a method employing PNGase F digestion, fluorescent labeling of released glycans, and analysis b

205 Simultaneous PEGylation and fluorescent labeling of sCT is also demonstrated, using

206 mbined visualization of bile acid uptake and fluorescent labeling of several NTCP variants indicated

207 em for conditional genetic manipulation with fluorescent labeling of single neurons for imaging.

208 duction platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics us

209 luorescence in a quantifiable manner and the fluorescent labeling of targeted transcripts.

210 Fluorescent labeling of the cysteines also indicated tha

211 argeted to the RyR2 cytoplasmic assembly via fluorescent labeling of the FKBP12.6 subunit.

212 After fluorescent labeling of the interacting partners and liv

213 amino acids into proteins and the specific, fluorescent labeling of the intracellular ultrastructure

214 Fluorescent labeling of the primer extension products wa

215 scription complexes by the incorporation and fluorescent labeling of the thymidine analog 5-ethynyl-2

216 sualization systems successfully achieve the fluorescent labeling of the viral envelope and proteins,

217 dies of microtubule dynamics tend to rely on fluorescent labeling of tubulin, with tracking accuracy

218 ), a surrogate for l-methionine, followed by fluorescent labelling of AHA-containing cellular protein

219 re network of SHR hearts combining gel-based fluorescent labelling of coronaries with a CLARITY-based

220 Fluorescent labelling of Nylon microfibers with Nile Red

221 ron cryotomography (ECT) and new methods for fluorescent labelling of peptidoglycan are allowing new

222 Cryotomography of purified sacculi and fluorescent labelling of PG in live cells confirmed that

223 Efficient fluorescent labelling of single and double-stranded DNA

224 As a result of the dense fluorescent labelling of the DNA, individual barcodes of

225 Fluorescent labelling of this hexapeptide showed that it

226 ttle as 1 h without the use of antibodies or fluorescent labels of any kind.

227 However, non-specific multi-site fluorescent labeling often results in a loss of native s

228 ne with fluorescence detection and require a fluorescent label on a smaller-sized binding partner.

229 target DNA strands to measure the impact of fluorescent labeling on duplex stability.

230 Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an

231 product and the subsequent immobilization of fluorescent labels on the microparticle surface.

232 In this report we show that quenched fluorescent labels on various LAMP primers can be used t

233 clude sample preparation, surface chemistry, fluorescent labels, optimizing the enzyme-substrate syst

234 tion were obtained using virally transfected fluorescent labeling or by immunolabeling after fixation

235 Without the need for fluorescent labeling or Coulter counting, the white bloo

236 atistics to be generated without the need of fluorescent labels or other modification groups.

237 ar conformation detection techniques rely on fluorescent labels or protein-specific binding agents to

238 ements without additional modules other than fluorescent labels or quenchers.

239 ithout any disturbances by molecular probes, fluorescent labels, or immobilization of molecules.

240 In case of fractional fluorescent labeling, our simulations predicted that the

241 Fluorescent labeled PAC were able to promote ExPEC agglu

242 We report single and double fluorescent-labeled PAC with one or two chlorine atoms d

243 nt of effect or tracking of (14)C-labeled or fluorescent-labeled paclitaxel.

244 Using fluorescent labels, PCNA is shown to increase the bindin

245 Fluorescent-labeled peanut and soy extracts were used to

246 site-specific modification of antibodies for fluorescent labeling, PEGylation, protein cross-linking,

247 human chorionic gonadotropin (hCG), by using fluorescent-labeled polyclonal antibodies.

248 Confocal imaging with fluorescent-labeled polymer solutions was used to explor

249 Our method uses serial imaging of endogenous fluorescent labeled presynaptic VAMP2 and postsynaptic P

250 red highly purified virus for the subsequent fluorescent labeling procedure, thus enabling STED imagi

251 s have created a pressing need for efficient fluorescent labeling procedures to visualize and detect

252 r low-noise real-time kinetic measurement of fluorescent-labeled protein binding.

253 asurements indicated that the transgenes for fluorescent-labeled proteins did not disrupt normal stom

254 CCK) pharmacophores that are connected via a fluorescent labeled, rationally designed synthetic linke

255 loured probes were obtained in a single tube fluorescent labelling reaction; and varying the ratios o

256 e spectrum, which is required by many common fluorescent labeling reagents.

257 Furthermore, tracking fluorescent-labeled red blood cells at the endocrine-exo

258 so binds to fibrinogen, as determined with a fluorescent-labelled SAA antibody and correlative light

259 h an optimized particle design and efficient fluorescent labeling scheme, we demonstrate subattomole

260 We have demonstrated that the choice of fluorescent label significantly affects the fractional c

261 The addition of the fluorescent label significantly stabilizes the DNA duple

262 Scanning fluorescent labeling sites throughout the domain showed

263 we present fusion protein- and intein-based fluorescent labeling strategies that can probe Fe-S clus

264 Fluorescent labeling studies showed that the constituent

265 le push-pull fluorogens which produce bright fluorescent labels suitable for single-molecule superres

266 Fluorescent labeling techniques have been widely used in

267 , fluorescence correlation spectroscopy, and fluorescent labeling techniques have enabled new experim

268 imit barrier, reliance of such techniques on fluorescent labeling technologies prohibits quantitative

269 cellular changes induced by stimuli such as fluorescent labeling, temperature change, starvation, an

270 strands differing only by the presence of a fluorescent label tethered to the 5' end of the solution

271 determining changes in the orientation of a fluorescent label that assumes a unique orientation in e

272 ical properties drive development and use of fluorescent labels that absorb and emit at longer wavele

273 al fluorescent properties and yielded stable fluorescent labels that could be easily activated and bi

274 Our system offers permanent fluorescent labels that reveal fine morphological detail

275 able to generate long overhangs suitable for fluorescent labeling through end-filling or other techni

276 roach that monitors the separation between a fluorescent-labeled TMS and fluorescent phospholipids di

277 highly specific genetic tag for attaching a fluorescent label to a protein of interest.

278 probes that contain both a radiotracer and a fluorescent label to allow for enhanced intraoperative d

279 olecule photobleaching and substoichiometric fluorescent labeling to determine the aggregation number

280 electrophysiology, immunohistochemistry, and fluorescent labeling to directly assess cholinergic syna

281 Here, we used sequence-specific fluorescent labeling to map the incorporation patterns o

282 that AHA incorporation permits site-specific fluorescent labeling to track voltage-dependent conforma

283 We used the bleaching of the fluorescent labels to determine the number of active flu

284 cules, such as nucleic acids or proteins and fluorescent labels to identify the target(s) of interest

285 imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far

286 Furthermore, the fluorescent-labeled universal aptamer used in this syste

287 to undergo conformational change and release fluorescent label upon interaction with the flow of the

288 are typically attributed to the influence of fluorescent labels used in smFRET and to the impact of h

289 man serum using gold nanoclusters (AuNCs) as fluorescent label was developed.

290 was used to compromise cell membranes, and a fluorescent label was used to monitor the extent, result

291 Fluorescent labeling was confirmed by fragmentation expe

292 In addition to fluorescent labeling, we demonstrate the utility of the

293 Only minor differences in fluorescent-labelling were observed between the dividing

294 fortunately, this method inherently requires fluorescent labeling which has several drawbacks.

295 on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with

296 onal chemical tag, which allows simultaneous fluorescent labeling with a green dye (tetramethylrhodam

297 We demonstrate their selective fluorescent labeling with respect to the proteome of liv

298 ther our understanding of complex processes, fluorescent labeling with visible light fluorescent prot

299 is shows the 3D relative movement of the two fluorescent labels with an accuracy of 30 nm.

300 proved that the dyes may serve as removable fluorescent labels with large Stokes shifts (>80 nm).