ライフサイエンスコーパス: fluorochrome

1 e for uniform labeling with a thiol-reactive fluorochrome.

2 ecule, thereby releasing previously quenched fluorochrome.

3 at terminates in a 7-amino-4-methyl-coumarin fluorochrome.

4 es a charge balanced and sterically shielded fluorochrome.

5 one detector (primary) is dedicated for one fluorochrome.

6 (AML) cells, all with the same near-infrared fluorochrome.

7 y, and each pool was labeled with a specific fluorochrome.

8 (6-methoxy-8-quinolyl)-p-toluene sulfonamide fluorochrome.

9 in the staining characteristics of the three fluorochromes.

10 dead yeast cells, which nonspecifically bind fluorochromes.

11 oncatameric DNA probe labelled with multiple fluorochromes.

12 seradish peroxidase (WGA-HRP) and up to four fluorochromes.

13 rimers that are 5'-end labeled with infrared fluorochromes.

14 d bacterial artificial chromosomes producing fluorochromes.

15 ease in pH1, measured using the pH-sensitive fluorochrome, 2',7'-bis(carboxyethyl)-5-(6)-carboxyfluor

16 ls was measured with the cationic lipophilic fluorochrome 3,3'-dihexyloxacarbocyanine iodide [DiOC(6)

17 d to measure cells labeled with five or more fluorochromes (a practice known as polychromatic flow cy

18 Germ Agglutinin (WGA) and a lipophilic blue fluorochrome, Ac-201, for the simultaneous imaging of ar

19 odified at the 5'- or at the 3'-end with Cy5 fluorochrome (acceptor).

20 The same performance values for the fluorochrome acid-fast bacillus smear were 33, 98, 62, a

21 The approach utilizes cycles of labeled fluorochrome addition, detection to count incorporations

22 was monitored using a dansylated polymyxin B fluorochrome agent.

23 n tumors, nonsilencing siRNA tagged with the fluorochrome Alexa 555 was encapsulated into DOPC liposo

24 first converted to an intermediate bearing a fluorochrome and amine groups, then reacted with either

25 nfluence of fixation and permeabilization on fluorochrome and antibody performance.

26 Using a calcium-sensitive fluorochrome and digital multichannel intravital microsc

27 MP-3653) represents a liposome tagged with a fluorochrome and functionalized with a PEG chain spacer

28 rypton was stained with multiple DNA-binding fluorochromes and a monoclonal anti-double-stranded DNA

29 le blocking troublesome interactions between fluorochromes and biomolecules.

30 tumors, producing images of distributions of fluorochromes and nanoparticles as well as tumor vascula

31 al and spectral measurements of a mixture of fluorochromes and the ability to differentiate relative

32 tions of a single Ab conjugated to different fluorochromes and thus in principle can be integrated in

33 ures consisting of biologics, radionuclides, fluorochromes, and CNT could be synthesized and designed

34 etic encoded tags that can be complexed with fluorochromes, and combinations of these probes are high

35 ial resolution, nanomolar sensitivity to NIR fluorochromes, and modest NIRF light attenuation through

36 ured ROI production with oxidation-sensitive fluorochromes, and monitored cell death by annexin V and

37 tion of HREC proliferation and staining with fluorochrome-annexin V showed numerous apoptotic HRECs.

38 Apoptosis was examined using fluorochrome-annexin V staining.

39 rom 63-88C3 and CAP63 labeled with different fluorochromes as positive and negative probes, respectiv

40 /7 and caspase-9 activities were measured by fluorochrome assays.

41 e fluorescence emission of the near-infrared fluorochromes attached to the N-termini of the dendritic

42 que (FMT) that enables rapid measurements of fluorochrome-based affinity tags in live xenograft model

43 ngivalis, and phagocytosis was measured in a fluorochrome-based assay by flow cytometry.

44 nd nitric oxide levels were measured using a fluorochrome-based assay.

45 (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine a

46 The 6-substituted indole-boron difluoride fluorochromes bearing electronically modulating substitu

47 To demonstrate PEG-fluorochrome shielding, fluorochrome-bearing peptide probes were synthesized, th

48 Although the transfer of small fluorochromes between them was rarely observed beyond se

49 omide colorimetric assay and the DNA-binding fluorochrome bis (benzimide) trihydro-chloride (Hoechst

50 uring the experiment, the animals were given fluorochrome bone markers for histomorphometry.

51 sistant, respectively), were loaded with the fluorochrome calcein and exposed to a range of concentra

52 We demonstrate that enzyme-activatable fluorochromes can be detected with high positional accur

53 teractions, rather than synthesizing de novo fluorochromes, can yield new approaches for the design o

54 Fluoro-Jade is an anionic fluorochrome capable of selectively staining degeneratin

55 -8, -9, and -12 activities were measured by fluorochrome caspase assays.

56 ding polymerase type, buffer conditions, and fluorochrome chemistries--a rational strategy for the se

57 each tagged with a specific fluorochrome or fluorochrome combination, has resulted in the differenti

58 in its application by the number of possible fluorochrome combinations, IMC uses isoptope-coupled ant

59 tibodies (anchor markers) in the appropriate fluorochrome combinations, in order to show the quantifi

60 within 10% of their mean for each individual fluorochrome concentration and were significantly distin

61 We observed at least a 10-fold increase of fluorochrome concentration in cyclophosphamide-sensitive

62 c catheter placed above wells of varying NIR fluorochrome concentration, varied markedly (>100%) with

63 Fluorochrome concentrations derived from FMT measurement

64 cant dissimilarities in signal from distinct fluorochrome concentrations.

65 y showed that the pDNA hybridized to one PNA-fluorochrome conjugate molecule.

66 sed using C3dg-biotin tetramers complexed to fluorochrome conjugated streptavidin and measured by flo

67 followed by secondary staining with anti-Ab fluorochrome-conjugated Abs to amplify fluorescence inte

68 d CD14(+) human monocytes were stained using fluorochrome-conjugated Abs to myeloid surface markers,

69 ow cytometry with basophil markers and eight fluorochrome-conjugated allergen component tetramers.

70 d, and naive animals were immunostained with fluorochrome-conjugated anti-FoxP3 and anti-CD4 monoclon

71 ese rare cells is to sort cells stained with fluorochrome-conjugated antibodies into fractions, then

72 d arborization of vessels labeled in vivo by fluorochrome-conjugated lectin with effective treatment.

73 pensions obtained from gingival tissue, with fluorochrome-conjugated monoclonal antibodies against CD

74 Tie2-GFP transgenic mice in combination with fluorochrome-conjugated monoclonal antibodies to vascula

75 different concentrations of a cocktail of 4 fluorochrome-conjugated monoclonal antibodies.

76 After tumor disaggregation, staining with a fluorochrome-conjugated monoclonal antibody, and flow cy

77 Construction of fluorochrome-conjugated peptide-I-A(b) class II tetramer

78 ion of epitope-specific CD4 T cells by novel fluorochrome-conjugated peptide-I-A(b) tetramers allows

79 Fluorochrome-conjugated peptide-MHC (pMHC) class I multi

80 Fluorochrome-conjugated peptide-MHC (pMHC) multimers are

81 f SCT and AT-SCT peptide-MHC complexes using fluorochrome-conjugated TCR multimers suggests that nona

82 On unfixed borreliae, fluorochrome-conjugated uPA bound to all species.

83 CD4 with specific monoclonal antibody (MoAb)-fluorochrome conjugates and for DNA polyploidization wit

84 Fluorochrome conjugates and UV microscopy were used to q

85 fferences in monoclonal antibodies and their fluorochrome conjugates, or failure of the total absolut

86 inylated, and tetramerized with streptavidin-fluorochrome conjugates.

87 rt a method to couple commercially available fluorochromes covalently and sequence-specifically to pD

88 the donor fluorochrome FITC and the acceptor fluorochrome Cy3.

89 ptides functionalized with the near-infrared fluorochrome Cy5.5 (the peptides facilitate the uptake o

90 pairs per millimeter and detection limit for fluorochrome Cy5.5 of 1-10 pmol.

91 The system is linear down to fluorochrome densities <<1 molecule/microm2.

92 injection of fluorescent tracer (Fluorogold; Fluorochrome, Denver, CO) into the superior colliculus t

93 loss (judged by lack of Fluorogold labeling; Fluorochrome, Denver, CO), adjacent areas with relative

94 as assessed by changes in bone geometry and fluorochrome-derived bone formation rates.

95 The single-fluorochrome detection limits achieved by the cumulative

96 ion was measured using the oxidant-sensitive fluorochrome dihydrorhodamine 123 (DHR-123).

97 dant production (using the oxidant-sensitive fluorochrome dihydrorhodamine-123) were monitored by int

98 nd 596) were labeled with a europium chelate fluorochrome (donor).

99 nced dynamic bone formation rates in in vivo fluorochrome double labeling experiments.

100 By using dual fluorochrome dyes (Hoechst and Nile Red) to locate nucle

101 retrograde labeling of RGCs with Fluorogold (Fluorochrome; Englewood, CO) was used.

102 protocol, using transgenic mouse brains with fluorochrome expression in microglia.

103 ferentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluor

104 ytometric analysis of FRET between the donor fluorochrome FITC and the acceptor fluorochrome Cy3.

105 animals were stained with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neu

106 followed by staining with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neu

107 ms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA.

108 cquisition of a series of images of multiple fluorochromes from multiple arrays occurs under computer

109 radiolabeled elements or sterically hindered fluorochrome groups, are usually employed to screen phos

110 Fluorochrome images and angiogenic marker CD31 staining

111 of multiple allergens coupled with distinct fluorochromes in a single-tube assay enabled rapid detec

112 emiacetal aglycon leaving group that tethers fluorochromes in close proximity, also positioning them

113 Continuous (1- to 5-minute) excitation of fluorochromes in the capillary lumen (light-dye treatmen

114 of rhodopsin's screening of autofluorescent fluorochromes in the RPE.

115 henol-glucuronide moiety (TrapG) to trap the fluorochromes in the vicinity of the active enzyme.

116 eroxidase (WGA-HRP) and up to four different fluorochromes in V2 labeled neurons and terminations in

117 p-nitrophenyl phosphonate-conjugated fluorochromes, including the very bright and stable quan

118 the 3' end of the first ODN and NIR acceptor fluorochromes (indodicarbocyanine Cy7 or, alternatively,

119 Caspase 3 activity was measured by a fluorochrome inhibitor of caspase (FLICA) assay.

120 -3, -8, and -9 activities were measured by a fluorochrome inhibitor of caspase (FLICA) assay.

121 aspase-9 and -12 activities were measured by fluorochrome inhibitor to caspase (FLICA) assays.

122 transcripts by the incorporation of a second fluorochrome into DNA templates promotes more robust and

123 Infusion of Fluo-4 AM, a calcium-sensitive fluorochrome, into the mouse circulation resulted in dye

124 nce resonance energy transfer (FRET) between fluorochromes introduced into these DNA molecules.

125 CNIR800, that carries a near-infrared (NIR) fluorochrome IRDye 800CW, with a quencher connected thro

126 dition, the assay sensitivities using direct fluorochrome label as the reporter, or alkaline phosphat

127 in that bound HLA-A*0201 were identified and fluorochrome-labeled A*0201 tetramers with each peptide

128 cific for Der p 1 were detected by using the fluorochrome-labeled allergen method.

129 d-type mice by intravenous administration of fluorochrome-labeled anti-E- and anti-P-selectin antibod

130 Neutrophil phenotype was determined using fluorochrome-labeled antibodies to CD16 and CD11b and as

131 Cells were characterized using fluorochrome-labeled antibodies to surface markers, intr

132 for suspensions of single cells labeled with fluorochrome-labeled antibodies, and 3-dimensional spati

133 rix is externalized and immediately bound by fluorochrome-labeled avidin present in the culture mediu

134 ng high-resolution confocal microscopy and a fluorochrome-labeled avidin probe, one can directly asse

135 n in real time by exploiting the capacity of fluorochrome-labeled avidin to stain degranulating cells

136 s supported by competitive binding assays of fluorochrome-labeled beta25-35 and Mac-1 antibodies (Wt.

137 d anti-CD11b) and (2) competitive binding of fluorochrome-labeled beta25-35 with anti-CD18 or anti-CD

138 cipient origin, is combined with multi-color fluorochrome-labeled CD epitope-specific monoclonal anti

139 itopes of the target are modified with short fluorochrome-labeled complementary oligonucleotides and

140 Physical proximity between fluorochrome-labeled CR3 and Fc gamma RIIIB on individua

141 Fluorochrome-labeled darTreg were identified and charact

142 Intracellular fluorochrome-labeled diamine oxidase (DAO) was used as a

143 alpha-subunit of E. coli RNA polymerase to a fluorochrome-labeled DNA fragment containing the rrnB P1

144 logy is protein-dependent association of two fluorochrome-labeled DNA fragments, which allows generat

145 sure both snapshot and finish-line images of fluorochrome-labeled DNA.

146 ling of both fixed and live spirochetes with fluorochrome-labeled Fab-CB2 and 11G1, and an immunoglob

147 proliferation of B-1a lymphocytes that bind fluorochrome-labeled Ft-LPS and the differentiation of t

148 populations were identified by staining with fluorochrome-labeled HLA tetramers and anti-CD19, CD27,

149 ults obtained with fluorogenic substrates or fluorochrome-labeled inhibitors of caspases.

150 se-1 activity (FAM-FLICA [carboxyfluorescein-fluorochrome-labeled inhibitors of caspases] caspase-1)

151 Fluorochrome-labeled Mabs were used in T-cell phenotypic

152 The assay uses fluorochrome-labeled monoclonal antibodies to HCMV immed

153 lymerase-mediated incorporation of different fluorochrome-labeled nucleotides (FdNTPs) was investigat

154 to mediate the consecutive incorporation of fluorochrome-labeled nucleotides into an array of large

155 t-dependent annealing of short complementary fluorochrome-labeled oligonucleotides attached to a pair

156 In vitro, PEG blocked the interactions of fluorochrome-labeled peptide probes with each other (abs

157 Upon mixing RNAP and fluorochrome-labeled promoter a time-dependent biphasic

158 some Arp-specific B cells, identified using fluorochrome-labeled rArp proteins, had the phenotype of

159 tinal ganglion cell layer cells (RGCLCs) and fluorochrome-labeled retinal ganglion cells (RGCs) on fl

160 Preservation of fluorochrome-labeled RGCs was assessed 2 weeks later.

161 rs followed by intracellular staining with a fluorochrome-labeled SLP-76-specific Ab.

162 Optical imaging with a fluorochrome-labeled version of the mAb showed colocaliz

163 ive, as characterized by irregular, punctate fluorochrome labeling and elevated proteoglycan.

164 old, respectively) associated with increased fluorochrome labeling and expression of mineralized tiss

165 d is based on biotinylation and streptavidin-fluorochrome labeling of APCs, followed by subsequent ac

166 Fluorochrome labeling of dentin in Dmp1-null mice showed

167 In vivo fluorochrome labeling revealed increased MAR and bone re

168 mputed tomographic (uCT) imaging and in vivo fluorochrome labeling were also used to quantify bone vo

169 In vivo fluorochrome labeling, histology, and immunohistochemica

170 Using fluorochrome labeling, the present study determined the

171 on chamber for PCR, which through the use of fluorochrome labelled probes allows the target molecules

172 Fluorochrome-labelled monoclonal antibodies were used in

173 ing propidium iodide, a nucleic acid-binding fluorochrome largely excluded by the intact cell membran

174 included donor NIR Cy5.5 indodicarbocyanine fluorochrome linked to the 3' end of the first ODN and N

175 nal spatiotemporal kinetics in spheroids for fluorochrome-loaded liposomes.

176 Fluorochromes localize to the tumor by taking advantage

177 Using PEG to block fluorochrome-mediated interactions, rather than synthesi

178 tensity into molecules of equivalent soluble fluorochrome (MESF) units.

179 alculated as molecules of equivalent soluble fluorochrome (MESF).

180 tandard units of mean equivalents of soluble fluorochrome (MESF).

181 ifferentiate relative concentrations of each fluorochrome mixture in real time.

182 ase-DNA template complex that considered the fluorochrome moiety of the FdNTPs and the linker length

183 g arms to be appended strategically over the fluorochromes of near-infrared cyanine heptamethine dyes

184 cular imaging, generally designed by placing fluorochromes on a cleavable substrate in close proximit

185 painting probes, each tagged with a specific fluorochrome or fluorochrome combination, has resulted i

186 e C2A domain of synaptotagmin I labeled with fluorochromes or a radioisotope binds to both apoptotic

187 ng Br-RNA was immunolabelled indirectly with fluorochromes or gold particles.

188 was performed without the use of antibodies, fluorochromes, or dyes, leaving the sorted spores in the

189 technique by staining tissue specimens with fluorochrome- or biotin-labeled GAGs to visualize the di

190 A was 68,171 molecules of equivalent soluble fluorochrome (P<0.0001), representing 55%+/-22%.

191 64 to 59,753 molecules of equivalent soluble fluorochrome per cell (MESF), as determined by flow cyto

192 nucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a s

193 ystem was validated with in vitro imaging of fluorochrome phantoms and in vivo fluorescence measureme

194 velopment and validation of simple models of fluorochrome point spread functions within the imaging s

195 graft copolymer bearing near-infrared (NIR) fluorochromes positioned on cleavable substrate sequence

196 ection depends on FRET efficacy and relative fluorochrome positions within ODN binding sites.

197 used to measure protein phosphorylation; the fluorochrome probe 2'7'-dichlorofluorescin diacetate was

198 to energy resonance transfer among the bound fluorochromes (quenching) but became brightly fluorescen

199 entary studies, nucleotides labeled with the fluorochrome R110 were incorporated into surface-elongat

200 Easy to find: magnetic nanoparticles bearing fluorochromes (red) that intercalate with DNA (green) fo

201 idin conjugated to magnetic nanoparticles or fluorochromes, respectively.

202 arrays, two triplicate hybridizations (with fluorochrome reversal) comparing RNAs from a fibroblast

203 A method to attach a fluorochrome sequence-specifically to supercoiled plasmi

204 e differential uptake of an (111)In-labeled, fluorochrome-shielded, integrin-binding RGD probe and a

205 ach for fluorescent probe design termed "PEG-fluorochrome shielding", where PEGylation enhances quant

206 To demonstrate PEG-fluorochrome shielding, fluorochrome-bearing peptide pro

207 blood compared with the intensity of the raw fluorochrome signal, which would vary +/-60%.

208 beled chromosome-painting probes with unique fluorochrome signatures onto human or mouse metaphase ch

209 polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellul

210 ealed through staining with the aniline blue fluorochrome, Sirofluor.

211 e use of recommended methods was as follows: fluorochrome stain for acid-fast bacillus microscopy (47

212 uberculosis, and 13 (38%) of these were also fluorochrome stain positive.

213 ochrome stain-positive specimens and 57% for fluorochrome stain-negative specimens.

214 LCR sensitivity was 100% for fluorochrome stain-positive specimens and 57% for fluoro

215 itutions with abnormal chest radiographs and fluorochrome stain-positive sputa were evaluated for tub

216 16S ribosomal RNA (16S rRNA) expression and fluorochrome staining with Mitotracker Red CMXRos (Delta

217 ression, live-cell imaging, and aniline blue fluorochrome staining.

218 er p 2 were biotinylated and conjugated with fluorochrome streptavidins as tetramers.

219 Using a fluorochrome-tagged Gpr85 construct expressed in native

220 amined the route of entry into mouse skin of fluorochrome-tagged or naked second-generation 2'-O-meth

221 The elongation products were labeled with fluorochrome-tagged probes and detected in a flow cytome

222 a murine reporter strain by "knocking-in" a fluorochrome, tandem-dimer Tomato (tdTom), into exon 1al

223 ice were stained with Fluoro-Jade B (FJB), a fluorochrome that binds specifically to degenerating fib

224 ligonucleotides), each of which containing a fluorochrome that can form a fluorescence resonance ener

225 nd feasible intraoperative molecular imaging fluorochrome that should be further evaluated in randomi

226 staining using Abs of interest labeled with fluorochromes that are compatible with HO342 and 7-AAD e

227 Here, we report a new series of fluorochromes that can be efficiently inactivated by a s

228 tary oligonucleotides that are modified with fluorochromes that could participate in fluorescence res

229 ility of an expanded range of antibodies and fluorochromes that have improved our ability to identify

230 as engineered by conjugating a near-infrared fluorochrome to a peptide ligand derived from the amino

231 duced a third probe labeled with a different fluorochrome to create a triple-probe/three-color system

232 cular tomography and a cathepsin-activatable fluorochrome to image autophagy in the heart in vivo aft

233 NA in alkaline conditions and uses PicoGreen fluorochrome to monitor denaturation.

234 group that facilitates the attachment of the fluorochrome to nucleophilic moieties located near beta-

235 ne is reacted with a maleimide moiety on the fluorochrome to produce a fluorescent conjugate which is

236 tem was tested by using a combination of the fluorochromes to confirm spectral separation.

237 -specific coupling of commercially available fluorochromes to DNA expression vectors while retaining

238 stigated by placing restricted injections of fluorochrome tracers, wheat germ agglutinin-horseradish

239 we found that the simple addition of an anti-fluorochrome unconjugated Ab during staining resulted in

240 we attempted to improve staining using anti-fluorochrome unconjugated primary Abs followed by second

241 y hypoxic rats were in vivo-labeled with DiI fluorochrome via liposome delivery and subsequently iden

242 diolabeling ((89)Zr-DNP) and a near-infrared fluorochrome (VT680) for microscopic and cellular valida

243 A NIR fluorochrome was conjugated to a cyclic peptide that bin

244 In tissue, 250 pmol of fluorochrome was easily detected during the 10-second im

245 rm (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and

246 ious pairs of proteins fused to two distinct fluorochromes were coexpressed in N. benthamiana leaf ti

247 Both Cy7 and 800CW fluorochromes were linked by using hydrophilic internucl

248 The different fluorochromes were readily distinguished with the system

249 markers and the corresponding antibodies and fluorochromes were selected based on their contribution

250 e case of Cy5.5-Cy7 and Cy5.5-800CW pairs of fluorochromes, which was sensitive to the relative posit

251 These fluorogenic dyes integrate a coumarin fluorochrome with the bioorthogonal trans-cyclooctene(TC

252 ergo an internal charge transfer to form new fluorochromes with longer pi-electron systems.