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1 f the exogenous enzyme on the dye results in fluorogenic activation of the dye, enabling fast voltage
2 es was retained, which was shown by in vitro fluorogenic activity and whole blood clotting assays.
5 termine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluore
7 l-5-yl)sulfane (BISMORX) as a thiol specific fluorogenic agent for live cell nonprotein thiol (NPSH)
8 90 nm suggesting a possibility of developing fluorogenic aldehyde-reactive probes based on ABAO frame
9 trations of fluorescein diphosphate (FDP), a fluorogenic alkaline phosphatase substrate, with and wit
10 The method is nonenzymatic, isothermal, and fluorogenic, allowing the direct detection of nucleic ac
11 with a newly developed, extremely sensitive fluorogenic alpha-tocopherol analogue (H4BPMHC), we repo
12 rotocol covers the chemical synthesis of the fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH (3-4 d), t
13 he preparation of the tryptophan (Trp)-based fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH and its in
16 a set of monolignol analogs gamma-linked to fluorogenic aminocoumarin and nitrobenzofuran dyes were
17 ermined both with auto-fluorescence and with fluorogenic amyloid markers, under confocal microcopy.
18 rein the synthesis and characterization of a fluorogenic analogue of ubiquinone designed to reversibl
19 hesis, and characterization of a two-segment fluorogenic analogue of vitamin K, B-VKQ, prepared by co
20 losterically activates ERAP1's hydrolysis of fluorogenic and chromogenic amino acid substrates but co
23 report here the preparation of a lipophilic fluorogenic antioxidant (Mito-Bodipy-TOH) that targets t
24 findings underscore the potential of the new fluorogenic antioxidant Mito-Bodipy-TOH to study the che
28 tion of the TM domain did not affect 2-AG or fluorogenic arachidonoyl, 7-hydroxy-6-methoxy-4-methylco
29 s that function by H2S-mediated reduction of fluorogenic aryl azides has emerged as one of the most c
30 e we describe a new mix-and-read homogeneous fluorogenic assay for the measurement of the AP-site cle
31 de a femtoliter reactor array device for the fluorogenic assay of neuraminidase, and incubated for mi
32 l data reported in this study along with the fluorogenic assay will be useful in substrate specificit
33 We previously described the development of fluorogenic assays for nucleic acid-modifying enzymes ba
34 and inexpensive platform for development of fluorogenic assays that do not require organic fluoropho
35 e capability of the system as a platform for fluorogenic assays was demonstrated through designing mo
37 ectron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversio
38 rk, genome mining combined with the use of a fluorogenic azide-based click probe led to the discovery
46 milieu to electrophiles, we have designed a fluorogenic BODIPY-acrolein probe, AcroB, that undergoes
48 e used as a highly selective chromogenic and fluorogenic chemosensor for the detection of cyanide in
53 vation of the fluorescence in situ through a fluorogenic conjugation step effectively constrains the
54 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorogenic core, which works as an environmentally sens
56 d-induced mechanical activation to release a fluorogenic coumarin payload from a polymer incorporatin
57 An RNA aptamer selected for binding to the fluorogenic cyanine dye, dimethylindole red (DIR), also
58 e design, synthesis and application of rotor-fluorogenic D-amino acids (RfDAAs), enabling real-time,
59 the design principles required for efficient fluorogenic design of these systems to function as fluor
61 te sensor provides a unique platform for the fluorogenic detection of PPi having a very low limit of
66 ds to the target of the Spinach aptamer, the fluorogenic dye DFHBI, and thereby induces a strong fluo
68 s indicate that alpha-CN-TO will be a useful fluorogenic dye in combination with specific RNA and pro
69 in (FAP) that binds a heavy atom-substituted fluorogenic dye, forming an 'on-demand' activated photos
70 irst time the application of a water-soluble fluorogenic dye, Thioflavin T (ThT), in a dual role of e
73 ssion sensitivity for OTA detection when the fluorogenic dyes induce a topology change in OTABA (para
78 single-chain variable fragments and activate fluorogenic dyes, which only become fluorescent upon act
81 ver parameters that can be tuned to maximize fluorogenic efficiency in bioconjugation reactions and r
84 vestigations led to the discovery of two new fluorogenic epsilon-N-succinyllysine-containing substrat
85 on embedding fluorescein dilaurate (FDL), a fluorogenic ester substrate, into the polyester matrix a
87 l applications, we have designed an infrared fluorogenic executioner-caspase reporter, which reveals
90 C-S bond coupling reaction enabled the first fluorogenic formation of phospholipids, which self-assem
91 tors had high affinity toward both synthetic fluorogenic FRET peptides (95%) and dentin powder substr
93 fluorescence of the immobilized TFP and the fluorogenic group provided a direct tool to distinguish
97 also demonstrate that dihydroresorufin, as a fluorogenic indicator, gives an improved temporal respon
98 ion of FEEM focused on the use of a specific fluorogenic indicator, resazurin, which is reduced to ge
102 s of qPCR instruments restrict the number of fluorogenic labels that can be differentiated by the ins
103 to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to
106 otides named malaswitches bind their cognate fluorogenic ligand (MG) exclusively when RNA-RNA kissing
107 such conformational changes was enabled by a fluorogenic ligand that fluoresces upon AgHalo forming s
113 is unknown, and therefore we used an in situ fluorogenic MMP sensor peptide to determine how drug tre
115 nomer 1 for the integration into chromo- and fluorogenic molecularly imprinted polymers (MIPs) was sy
116 nsidered as the brightest polarity-sensitive fluorogenic molecules reported to date, having approxima
119 ivo fluorescence imaging, we developed a new fluorogenic near-infrared probe that can be activated by
120 ion manifold after reacting with mM order of fluorogenic o-phthalaldehyde (OPA)/thiol reagent (pH 10.
121 living cells using molecular beacons (MBs) - fluorogenic oligonucleotide probes - with minimal target
124 arations conventionally rely on chromogenic, fluorogenic, or redox properties for analyte detection t
125 BLAS consist of two RNA or DNA strands and a fluorogenic organic dye added as a buffer component.
128 eport the rational design of a highly stable fluorogenic peptide (termed Apo-15) that selectively sta
129 binant TPPE was able to process the quenched fluorogenic peptide and to cleave the acidic domain at l
132 was guided by an assay that used a quenched fluorogenic peptide comprising the amino acid sequence b
136 intact but hyperactive in the hydrolysis of fluorogenic peptide substrates and the degradation of po
137 orbent Surface Area" that uses conversion of fluorogenic peptide substrates to measure the intrinsic
139 adation of other MMP-9 substrates, such as a fluorogenic peptide, alphaB crystalline, or nonmuscular
140 detailed evaluation of two prodrug-inspired fluorogenic peptides designed to be efficient and select
141 Results of kinetics analysis with synthetic fluorogenic peptides indicated that P1'-P4', the C-termi
143 nching photo-induced electron transfer (PET) fluorogenic primers for the detection of P. falciparum a
147 k capacity, and in part by time-dependent RA-fluorogenic probe conjugate degradation by cellular prot
148 In the second experimental scenario, the RA-fluorogenic probe conjugate is pre-formed in a cell by s
149 Removal of the stress results in apparent RA-fluorogenic probe conjugate re-folding, mediated in part
153 uorescein diacetate) is the most widely used fluorogenic probe for the detection of general oxidative
155 e of multiplexing may be increased by adding fluorogenic probe pairs with other emission wavelengths.
156 ction relationships in Dbr1 enzymes, and the fluorogenic probe permits high-throughput screening for
160 esults highlight the potential of B-VKQ as a fluorogenic probe to study electron transfer and transpo
162 demonstrated by the conditional release of a fluorogenic probe using mitochondrial pretargeting.
164 with activity: A hydrogen sulfide selective fluorogenic probe, 7-azido-4-methylcoumarin (AzMC), serv
168 demonstrated through the preparation of two fluorogenic probes for the selective detection of biothi
169 ld, we demonstrate the ability to synthesize fluorogenic probes for the sensitive and selective detec
172 oteases were challenged to cleave bead-bound fluorogenic probes of citrulline-dependent proteolysis,
175 fferent amplification products is the use of fluorogenic probes that emit at different wavelengths (c
177 is shown that such molecules can be used as fluorogenic probes to detect the template-dependent bind
178 and hydropropidine ([Formula: see text]) are fluorogenic probes used for the detection of the intra-
181 The design, synthesis, and evaluation of fluorogenic probes with high specificity for BlaC, a bio
182 eraction between the ROS/RNS species and the fluorogenic probes, as follows: superoxide using hydroet
190 esented here exploits the solvatochromic and fluorogenic properties of nile red to extract both the e
191 ow-based assay, which takes advantage of the fluorogenic properties of phosphorylated coumaryl amino
197 nalytical applications, such as studies with fluorogenic protease substrates or nucleic acids probes.
202 within an organelle of choice, using a fast fluorogenic reaction between a tetrazine-functionalized
203 edox reaction of interest to a complementary fluorogenic reaction converting an electrochemical signa
209 ances in using single-molecule microscopy of fluorogenic reactions to examine and understand the spat
210 eic acid probes, which show highly selective fluorogenic reactivity when annealed to their respective
212 need for fixatives or washing steps for non-fluorogenic reagents, and by the low levels of free calc
213 However, the use of DCFH2-DA, as many other fluorogenic redox probes, is mainly confined to the dete
214 tion of single redox events are the use of a fluorogenic redox species and the nanoconfinement and hi
216 coupling a conventional redox reaction to a fluorogenic reporter reaction on a closed bipolar electr
217 e this limitation, here we describe XY-69, a fluorogenic reporter that preferentially partitions into
219 at facilitates turnover amplification of the fluorogenic response in nucleic acid-templated reactions
220 Metalloporphyrin 3 shows a multiple channel fluorogenic response toward fluoride and cyanide and als
221 acid) through sharp colorimetric as well as fluorogenic responses even conspicuous with the naked ey
222 aptamers, with emphasis on in vitro-selected fluorogenic RNA aptamers and their different modes of li
224 rt the green-fluorescent Spinach or Broccoli fluorogenic RNA aptamers into metabolite-regulated senso
231 rially relevant polymers lack chromogenic or fluorogenic signatures, precluding use of spectroscopy-b
233 to experimentally validate this scheme, the fluorogenic species resazurin is chosen as a fluorescent
234 ine dye thiazole orange (TO) is a well-known fluorogenic stain for DNA and RNA, but this property pre
235 n we describe the development of a family of fluorogenic stilbene-based vinyl amides and vinyl sulfon
236 ultifaceted behavior, which depends upon the fluorogenic substituent, the solvent, and the basicity o
237 g the lysophosphatidylcholine-like substrate fluorogenic substrate 3 (FS-3) and approximately 10,000
238 te specificity of human Sulf-1 employing the fluorogenic substrate 4-methylumbelliferyl sulfate (4-MU
239 iluted 20000-fold into buffer containing the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacrid
240 d with a solution containing glucose and the fluorogenic substrate amplex red through the engineered
245 plication of a novel type of chromogenic and fluorogenic substrate for protease detection is describe
246 plarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the p
249 ts, containing varying concentrations of the fluorogenic substrate resorufin beta-d-galactopyranoside
252 ed components are mixed with an enzyme and a fluorogenic substrate that permits to follow the enzymat
255 el in human plasma, employing ecarin and the fluorogenic substrate, resulted in improvement of the de
257 tral enzyme of this network, by developing a fluorogenic substrate-based method for time- and space-r
259 ded MBL)) and the identification of suitable fluorogenic substrates (umbelliferone-derived cephalospo
260 e expression were assessed by using specific fluorogenic substrates and macroarrays, respectively.
261 In this work, we report readily synthesized fluorogenic substrates enabling enzyme-economical evalua
262 ethylrhodamine) have been used previously as fluorogenic substrates for a number of DNA modifying enz
263 r enzyme fluorescence (REF) that uses custom fluorogenic substrates for bacterial enzymes allows rapi
266 trates are required in costly amounts, while fluorogenic substrates require an additional apparatus (
269 n droplet microfluidic screening systems use fluorogenic substrates to measure enzymatic activities w
270 Mildly permeabilized cells were exposed to fluorogenic substrates to monitor the activity of intrac
280 In this study, we report the synthesis of fluorogenic taccalonolide probes that maintain the nativ
281 on of the glycans with either chromogenic or fluorogenic tags for optical measurement or isotopic tag
283 fficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach.
284 e believe highly selective proximity-induced fluorogenic tetrazine reactions could find broad uses in
285 looctene-containing lipids are tagged with a fluorogenic tetrazine reagent via a no-rinse, inverse el
286 cid derivative with a ligation reaction of a fluorogenic tetrazine, allowing for the imaging of sialy
289 proteins for tracking and imaging by use of fluorogenic tetrazines that become strongly fluorescent
290 ween 7-azabenzonorbornadiene derivatives and fluorogenic tetrazines that facilitates turnover amplifi
292 tleneck and report on the development of new fluorogenic transmembrane peptide substrates, which are
296 microtubules in live mammalian cells via the fluorogenic, two-photon-triggered photoclick chemistry w
297 bioprobe by integrating tetraphenylsilole, a fluorogenic unit with aggregation-induced emission (AIE)
298 The anion binding tendencies of the two fluorogenic ureas L(1)H and L(2)H, containing the 2-anth
299 neither from literature nor from results in fluorogenic vesicles and thus support the importance of