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1 difluoro-4-methylumbelliferyl phosphate as a fluorogenic substrate.
2 5) value of 342 microM were obtained for the fluorogenic substrate.
3 red for maximal enzymatic activity against a fluorogenic substrate.
4 sured in transduced cells and media, using a fluorogenic substrate.
5 bstrate poly(ADP-ribose) polymerase and of a fluorogenic substrate.
6 ta-galactosidase through its activation of a fluorogenic substrate.
7  low micromolar inhibitory potency against a fluorogenic substrate.
8 in as the enzyme and BODIPY FL casein as the fluorogenic substrate.
9 focal fluorescence microscopy using a Bodipy fluorogenic substrate.
10 ivities of KLK5 and KLK7 were compared using fluorogenic substrates.
11 r aminopeptidase activity against many model fluorogenic substrates.
12 cted and quantitated by flow cytometry using fluorogenic substrates.
13 eactions is often limited by the need to use fluorogenic substrates.
14  human sirtuin hydrolases against a panel of fluorogenic substrates.
15 igher chymotrypsin-like activity measured on fluorogenic substrates.
16 the cleavage of macromolecular and synthetic fluorogenic substrates.
17  caspase-12 when incorporated into synthetic fluorogenic substrates.
18 iety of intracellular enzymes with available fluorogenic substrates.
19  ratio at a given analyte concentration with fluorogenic substrates.
20 inatorial substrate libraries and individual fluorogenic substrates.
21  cPLA2 showed 20-fold higher activity toward fluorogenic substrates, 1-O-(1-pyrenedecyl)-2-arachidono
22 ing enzyme (IDE)-catalyzed hydrolysis of the fluorogenic substrate 2-aminobenzoyl-GGFLRKHGQ-ethylened
23 parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-N
24 ntained heat-labile sialidase activity for a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-D
25 g the lysophosphatidylcholine-like substrate fluorogenic substrate 3 (FS-3) and approximately 10,000
26 te specificity of human Sulf-1 employing the fluorogenic substrate 4-methylumbelliferyl sulfate (4-MU
27 fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-gluc
28  analysis of xylanase activity using a novel fluorogenic substrate, 6,8-difluoro-4-methylumbelliferyl
29 e assay measures the rate of hydrolysis of a fluorogenic substrate-6,8-difluoro-4-methylumbelliferyl
30 atalytic specificity was determined with the fluorogenic substrate: 6-carboxyfluorescein approximatel
31 the rat theta 2-2 enzyme (rGSTT2-2) with the fluorogenic substrate 7-amino-4-chloromethyl coumarin (C
32 e H(2)O(2)-supported oxidation of the marker fluorogenic substrate 7-ethoxy-4-trifluoromethylcoumarin
33 iluted 20000-fold into buffer containing the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacrid
34 continuously monitored using a validated MMP fluorogenic substrate, a microdialysis system placed wit
35 ctions of Staphylococcus aureus sortase with fluorogenic substrate Abz-LPETG-Dnp in the presence or a
36 e proteases were characterized using kinetic fluorogenic substrate activity assays.
37 reening of a library of 137,180 tetrapeptide fluorogenic substrates against the T. brucei 20 S protea
38                                              Fluorogenic substrates allow for direct measurement of e
39                           The cell-permeable fluorogenic substrate allows single-cell cloning of cell
40 d with a solution containing glucose and the fluorogenic substrate amplex red through the engineered
41  engineered peroxidase APEX2 to activate the fluorogenic substrate Amplex UltraRed (AUR).
42 f dextran sulphate (DXS)-stimulation using a fluorogenic substrate and capillary immunoassays to quan
43 ndividual reaction vessels containing excess fluorogenic substrate and catalyzed the production of a
44 centrates, procoagulant lipid vesicles and a fluorogenic substrate and triggered with tissue factor (
45                                Together, the fluorogenic substrates and antibody aldolases provide re
46     Di- and tetra-oligopeptides were used as fluorogenic substrates and irreversible/competitive inhi
47 e expression were assessed by using specific fluorogenic substrates and macroarrays, respectively.
48                       Subsequent stamping of fluorogenic substrates and time-lapse imaging allows det
49 ghlight possible limitations associated with fluorogenic substrates and Ubl activity-based probes and
50 to detect the production of thrombin using a fluorogenic substrate, and (iv) titration of argatroban
51 al-Try-7 amino-4 methyl coumarin, a specific fluorogenic substrate, and Cbz-Leu-Leu-Tyr-CHN2, a speci
52 cs of several commonly used FRET substrates, fluorogenic substrates, and six of the 11 reported SARS-
53    Values of k(cat)/K(M) with hypersensitive fluorogenic substrates are 10(4) and 10(2)-fold lower th
54                                              Fluorogenic substrates are emerging tools that enable st
55                          The hydrolysis of a fluorogenic substrate, Arg-528/Asp-575 < Lys-528/Asp-575
56 e compared with pro-memapsin 2 using two new fluorogenic substrates, Arg-Glu(5-[(2-aminoethyl)amino]n
57  as a marker and the design of BlaC-specific fluorogenic substrates as probes for Mtb detection.
58 rance of peptidase activity that cleaved the fluorogenic substrates Asp-Glu-Val-Asp-aminotrifluoromet
59 ity was monitored in cells after PDT using a fluorogenic substrate, (Asp-Glu-Val-Asp)-AFC (7-amino-4-
60  protease activity in extracts measured by a fluorogenic substrate assay.
61                                        Using fluorogenic substrate assays, Western blotting, cathepsi
62                          After addition of a fluorogenic substrate at a defined time, the relative co
63                                              Fluorogenic substrates based on 4-methylumbelliferone (4
64 tral enzyme of this network, by developing a fluorogenic substrate-based method for time- and space-r
65 like activity as assessed by the cleavage of fluorogenic substrate benzyloxycarbonyl-DEVD-7-amido-4-(
66 .02 s-1 for the enzymatic hydrolysis of this fluorogenic substrate by FIV PR under the conditions of
67 ress curves of the hydrolysis of a sensitive fluorogenic substrate by FXIa in the presence of PN-2 to
68                         Upon cleavage of the fluorogenic substrate by SPase I, the fluorescent intens
69 : see text]-galactosidase activity using the fluorogenic substrate, C[Formula: see text]FDG; and auto
70  The strategy utilized in the design of this fluorogenic substrate can be applied in future endeavors
71 ps for performing enzymatic assays for which fluorogenic substrates cannot easily be designed.
72               We synthesized and optimized a fluorogenic substrate capable of reporting on granzyme B
73       Fusion was detected by cleavage of the fluorogenic substrate CCF2 by beta-lactamase-Vpr incorpo
74 designated REFtb, along with a more specific fluorogenic substrate, CDG-3.
75 ate cleavage pattern of human AMCase against fluorogenic substrates, chitobiose-4-methylumbelliferyl
76 , while matriptase activity was monitored by fluorogenic substrate cleavage.
77                                              Fluorogenic substrates composed of a fluorophore possess
78                    Turnover was observed via fluorogenic substrate conversion and fluorescence micros
79                                          The fluorogenic substrates detect activation at much lower c
80  forms by immunoblots and by cleavage of the fluorogenic substrate DEVD-AFC.
81 tivity, as measured in cell lysates with the fluorogenic substrate DEVD-AMC, was elevated almost imme
82  In this work, we report readily synthesized fluorogenic substrates enabling enzyme-economical evalua
83 this paper, we describe the development of a fluorogenic substrate for 17beta-hydroxysteroid-dehydrog
84 h ALDH activity, detected using Aldefluor, a fluorogenic substrate for ALDH.
85 ge of DEVD-aminomethylcoumarin (DEVD-AMC), a fluorogenic substrate for caspases 2, 3, and 7; in contr
86                                      A novel fluorogenic substrate for continuous feline immunodefici
87  using pH-sensitive fluorescent probes and a fluorogenic substrate for cysteine proteases showed that
88 no, 4-methyl coumarin amide (AAMCA), a novel fluorogenic substrate for FAAH, was designed and synthes
89                                            A fluorogenic substrate for HIV-1 protease was designed an
90  HN-299 was identified as a highly selective fluorogenic substrate for hSULT1E1.
91                                      Using a fluorogenic substrate for phospholipase A(2) (PLA(2)), w
92 plication of a novel type of chromogenic and fluorogenic substrate for protease detection is describe
93                      We have developed a new fluorogenic substrate for the alpha-glucosidase enzyme a
94 cein di-beta-d-galactopyranoside, which is a fluorogenic substrate for the intracellular enzyme beta-
95                                        A new fluorogenic substrate for the specific detection of orga
96 the identification and characterization of a fluorogenic substrate for tumor necrosis factor-alpha co
97 re assayed with a novel, membrane-impermeant fluorogenic substrate for vp165, we found that insulin s
98 ethylrhodamine) have been used previously as fluorogenic substrates for a number of DNA modifying enz
99 r enzyme fluorescence (REF) that uses custom fluorogenic substrates for bacterial enzymes allows rapi
100 k through development of near-infrared (NIR) fluorogenic substrates for beta-lactamase, an enzyme exp
101                                Analysis with fluorogenic substrates for caspase activity confirmed th
102              Here we report the synthesis of fluorogenic substrates for glycosidases based on a sulfo
103 egy was adopted to construct highly specific fluorogenic substrates for hSULT1E1.
104 ort the rational design of the near-infrared fluorogenic substrates for human GzmA and mouse GzmA.
105 bes a design of cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase expres
106 or engineering highly specific and sensitive fluorogenic substrates for target conjugative enzyme(s),
107                               A series of 54 fluorogenic substrates have been synthesized and evaluat
108 ully developed colorectal neoplasia-specific fluorogenic substrates, highlighting the ED substrate as
109 plarily demonstrated for the cleavage of the fluorogenic substrate hydrodabcyl-Ser-Phe-EDANS by the p
110 due to the complication involving the use of fluorogenic substrates in in vitro assays.
111 ating and neighboring cells using orthogonal fluorogenic substrates in various mixed cell systems.
112  reaction to convert a novel, cell-permeable fluorogenic substrate into fluorescein within living cel
113                          Therefore, this new fluorogenic substrate is a useful tool for the alpha-glu
114 ed lipid vesicles, and their reaction with a fluorogenic substrate is probed.
115                The rate of hydrolysis of the fluorogenic substrates is proportional to enzyme concent
116 the widely used Amplex Red, as well as other fluorogenic substrates, is often overlooked.
117           In this paper, we describe a novel fluorogenic substrate, KLTFGTVK(Abz)PVQAIAGY(NO2)EWL, in
118 tered the interaction of the enzyme with the fluorogenic substrate (Leu-Arg-Gly-Gly-NH)2-rhodamine.
119  agents led to the cleavage of the caspase-4 fluorogenic substrate, LEVD-7-amino-4-trifluoromethylcou
120                          Using combinatorial fluorogenic substrate libraries, the P1-P4 substrate spe
121 ty was assayed by monitoring cleavage of the fluorogenic substrate Mca-RPPGFSAFK(Dnp)-OH, a derivativ
122                           HRP first oxidizes fluorogenic substrate molecules like Amplex Red to radic
123 ochemistry, immunoblots, and cleavage of the fluorogenic substrate N-benzyloxycarbonyl-Asp-Glu-Val-As
124 ghly sensitive, selective, and tight binding fluorogenic substrate of a copper amine oxidase that is
125                            We report a novel fluorogenic substrate of bovine plasma amine oxidase (BP
126                            The 14-amino-acid fluorogenic substrate of sequence RALTK(Abz) VQ approxim
127 Tx was detected, as shown by the cleavage of fluorogenic substrates of the proteasome.
128 ity of rat nardilysin was investigated using fluorogenic substrates of the type 2-aminobenzoyl-GGX(1)
129  culture by introducing a combination of two fluorogenic substrates or a fluorogenic and a luminogeni
130 ty was comparable with results obtained with fluorogenic substrates or fluorochrome-labeled inhibitor
131                          The chromogenic and fluorogenic substrates, p-nitrophenyl-alpha-D-glucopyran
132                    The novel chromogenic and fluorogenic substrates, p-nitrophenyl-beta-D-glucopyrano
133                                              Fluorogenic substrate probes have been used to measure n
134 (Danio rerio) CYP1 isoforms with a series of fluorogenic substrate probes was determined by the rate
135 6 kDa proteolytic fragments with a synthetic fluorogenic substrate produced hyperbolic substrate vers
136 le Michael or Diels-Alder reactions of these fluorogenic substrates provide products of significantly
137 e in fluorescence on caspase cleavage of the fluorogenic substrates ranged from 40 to 83 depending on
138                                        These fluorogenic substrates release highly fluorescent BODIPY
139 veloped a high-throughput assay based on the fluorogenic substrate reporter WH-15.
140 Ti(2) and i(2)ANDNOTi(1) gates that cleave a fluorogenic substrate, reporting through an increase in
141 cs an i(1)ANDi(2) gate and cleaves the other fluorogenic substrate, reporting through an increase in
142 trates are required in costly amounts, while fluorogenic substrates require an additional apparatus (
143 ts, containing varying concentrations of the fluorogenic substrate resorufin beta-d-galactopyranoside
144 el in human plasma, employing ecarin and the fluorogenic substrate, resulted in improvement of the de
145 ave combined the GzB substrate with a second fluorogenic substrate selective for caspase 3 to allow b
146  the TF.factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the catalytic efficien
147 s was developed using the thrombin-specific, fluorogenic substrate SN-59 (100 muM).
148 y inhibit serine protease activity against a fluorogenic substrate specific for TLSPs.
149           The preparation was active against fluorogenic substrates specific for cathepsin D and E an
150 s by fluorescence polarization assay against fluorogenic substrates specific for MMPs or TLSPs.
151       Calpain activity was assayed using the fluorogenic substrate Suc-Leu-Leu-Val-Tyr-7-amino-4-meth
152                       Data from both sets of fluorogenic substrates supported the contribution of a P
153                                              Fluorogenic substrate tests provided a sensitive and sim
154          Our objective was to create a novel fluorogenic substrate that could be used for real-time d
155 ed components are mixed with an enzyme and a fluorogenic substrate that permits to follow the enzymat
156                                     However, fluorogenic substrates that function within live cells a
157  compounds should provide a diverse range of fluorogenic substrates that may be used to rapidly scree
158      A large proportion of studies relies on fluorogenic substrates that mimic the chemical propertie
159                                          The fluorogenic substrates that we describe and their sequen
160                          We show here, using fluorogenic substrates, that FZ treatment leads to the i
161            When loaded with a cell-permeable fluorogenic substrate, the cell library simultaneously r
162 n droplet microfluidic screening systems use fluorogenic substrates to measure enzymatic activities w
163   Mildly permeabilized cells were exposed to fluorogenic substrates to monitor the activity of intrac
164  low micromolar range) in competition with a fluorogenic substrate toward a recombinant form of the t
165 dish peroxidase, patterned downstream, where fluorogenic substrate turnover was recorded.
166 ded MBL)) and the identification of suitable fluorogenic substrates (umbelliferone-derived cephalospo
167                          A validated MT1-MMP fluorogenic substrate was infused through the microdialy
168 hancement of precision and sensitivity using fluorogenic substrates was demonstrated in a direct-bind
169                        The efficiency of the fluorogenic substrates was exemplified by inhibitor scre
170                     Using a plasmin-specific fluorogenic substrate, we developed a plasmin generation
171                                      Using a fluorogenic substrate, we quantified cathepsin D activit
172                                          The fluorogenic substrates were compared to chromogenic subs
173                                     eighteen fluorogenic substrates were designed based on literature
174                                          Two fluorogenic substrates were prepared, one using a covale
175                               Hypersensitive fluorogenic substrates were used to determine steady-sta
176 or the variants were determined with a novel fluorogenic substrate, which was designed to match the n
177 generation of fluorescent molecules from the fluorogenic substrate with the assistance of Fe(3)O(4) n
178    ANG cleaves a dinucleotide version of the fluorogenic substrates with a k(cat)/K(M) value of 61 M(
179  analysis of a series of otherwise identical fluorogenic substrates with Lys at P1 and different resi
180                                              Fluorogenic substrates with P1, P2, and P4 basic amino a
181 ge by purified mutant enzymes of a series of fluorogenic substrates with systematic changes in P(4) a
182 ently monitored with peptidic chromogenic or fluorogenic substrates, with certain peptide sequences i
183 ot protect monocytes from apoptosis, and the fluorogenic substrate YVAD-AFC failed to show an increas
184 ssue section directly in a cuvette using the fluorogenic substrate Z-arg-arg-AMC.
185 latin zymography in SDS-PAGE, and by in situ fluorogenic substrate zymography in RPE/choroid sections

 
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