ライフサイエンスコーパス: fluorometric

1 Caspase-3 fluorometric activity assays as well as immunoblot analy

2 ADCC was determined by using a fluorometric ADCC assay, before and after removal of pla

3 X-OH, resulting in distinct colorimetric and fluorometric alterations.

4 Western blot and fluorometric analyses indicated the loss of two differen

5 Histochemical and fluorometric analyses of GUS expression revealed that th

6 Microscopic and fluorometric analyses were used to distinguish the exoge

7 ted by delayed recovery of dsDNA analyzed by fluorometric analysis of DNA unwinding and the more exte

8 d from DNA single-strand lesions measured by fluorometric analysis of DNA unwinding.

9 monitored 54 livers during HOPE in terms of fluorometric analysis of released mitochondrial flavin (

10 ecules, the embryos were lysed gently, and a fluorometric analysis of their contents was performed.

11 Fluorometric analysis using the mitochondrial probes non

12 Using fluorometric and biochemical assays, we studied Cas9/gui

13 Supporting the quantitative fluorometric and colorimetric assays, size exclusion chr

14 erein, for the first time, we have developed fluorometric and colorimetric dual-mode nanoprobe derive

15 Both these fluorometric and colorimetric methods have been successf

16 Phagocytosis was assayed by fluorometric and colorimetric techniques.

17 ytes were identified by absorbance patterns, fluorometric and electrochemical detection. and comparis

18 Fluorometric and electrophysiological estimates of local

19 n in G-buffer into single filaments based on fluorometric and EM observations.

20 tion FRET experiments and protein microarray fluorometric and FRET assays.

21 omocysteine and folate were measured by HPLC-fluorometric and microbiological methods, respectively.

22 roxide (H(2)O(2)) production was measured by fluorometric and polarographic methods.

23 In comparison with other fluorometric and spectrophotometric assays for the detec

24 d) of an ODS II Spherisorb column, with both fluorometric and spectrophotometric detection.

25 A flow fluorometric approach to study cationic lipoid-DNA compl

26 ease ATP upon mechanical stimulation using a fluorometric approach.

27 gen peroxide production was observed both by fluorometric as well as by SECM measurements.

28 Herein, a peculiar fluorometric as well as smartphone-assisted RGB-relied s

29 e activity of MazFSa, including a continuous fluorometric assay and a gel-based cleavage assay.

30 reverse transcriptase was determined using a fluorometric assay and a poly(A) homopolymer as a templa

31 were screened for MMP-9 inhibitors, using a fluorometric assay and gelatin zymography.

32 Fluorometric assay and zymography showed that adhesives

33 ecular Probes, Eugene, OR) was measured by a fluorometric assay at 485 nm excitation and 530 nm emiss

34 e describes a simple, inexpensive, and rapid fluorometric assay based on the ability of dendrimers to

35 nts in serum samples were determined using a fluorometric assay based on the dye 6-carboxyfluoroscein

36 recombinant human PLTP were studied using a fluorometric assay based on the excimer fluorescence of

37 A fluorometric assay based upon the enzymatic transphospho

38 ccumulation of NADH, demonstrating that this fluorometric assay effectively monitors calcium-dependen

39 more sensitive than the currently available fluorometric assay for enzyme activity.

40 rophicum homologue (MthK) and a stopped-flow fluorometric assay for fast channel activation, we show

41 We present a continuous-read, fluorometric assay for high-throughput analysis of gluta

42 A rapid enzymatic fluorometric assay for measuring D-arabinitol in serum w

43 A fluorometric assay for mitochondrial membrane potential

44 The first direct and continuous fluorometric assay for monoamine oxidase B (MAO B) has b

45 A continuous, fluorometric assay for pectin methylesterase (PME) activ

46 We have developed a novel HPLC-based fluorometric assay for serine hydroxymethyltransferase a

47 A continuous fluorometric assay for tryptophan hydroxylase activity b

48 ase activity is not detectable by a standard fluorometric assay in C3H/HeOuJ (C3H) mice homozygous fo

49 Using a fluorometric assay in conjunction with solubilized recep

50 The fluorometric assay is carried out at an excitation wavel

51 determined using formaldehyde and creatinine fluorometric assay kits.

52 RP1 clusters was tested in a receptor-ligand fluorometric assay made by immobilizing soluble LRP1 "mi

53 and bacillus viability as determined using a fluorometric assay of bacterial viability and CFU determ

54 idase IV (DPPIV) activity was quantified via fluorometric assay of whole vessel homogenate.

55 y contaminants by ContamSPOT colorimetric or fluorometric assay on a thin layer chromatography (TLC)

56 This paper describes an in vitro fluorometric assay system for protein splicing based on

57 CPP32-like activity directly in an in vitro fluorometric assay system, although z-DEVD-fmk showed mu

58 purified protein was monitored by a coupled fluorometric assay that employed purified protoporphyrin

59 uantum dots (QDs) have been used in a simple fluorometric assay to detect single cells of the pathoge

60 ese concerns, employing a direct, cell-based fluorometric assay to investigate the regulation of TACE

61 ng CO2 flux across membranes, we developed a fluorometric assay to measure CO2 entry into vesicles.

62 We used an automated enzymatic fluorometric assay to measure serum DA/creatinine ratios

63 A fluorometric assay using nitrate reductase and the NADPH

64 to NA inhibitors (NAIs) was evaluated with a fluorometric assay using the 2'-(4-methylumbelliferyl)-a

65 A cell-based fluorometric assay was used to measure intracellular Ca(

66 A fluorometric assay was used to study WRN helicase kineti

67 ent of VWF-A2 (FRETS-VWF73) as determined by fluorometric assay, and enhanced cleavage of ultralarge

68 -like protease activity was measured using a fluorometric assay, and for the in situ detection of cas

69 ycin and Zn(II) was calculated using a novel fluorometric assay, and NMR was used to identify the bin

70 o internalize anti-CA19.9 antibodies using a fluorometric assay, and xenografts of the same lines wer

71 olipase A2 (sPLA2) activity, measured with a fluorometric assay, is low at birth, but increases progr

72 Using a fluorometric assay, it was determined that zopolrestat,

73 Using fluorometric assay, the detection limit (DL) for As (III

74 (PARP) proteolysis and a specific caspase-3 fluorometric assay, was inhibited by ischemic preconditi

75 glycation end products were assessed with a fluorometric assay.

76 erol levels were determined using a standard fluorometric assay.

77 m mobilization studies were done utilizing a fluorometric assay.

78 Caspase-9 activity was assessed by a fluorometric assay.

79 oAlert Mitochondrial kit, and caspase 9 by a fluorometric assay.

80 d its antioxidant capacity was measured in a fluorometric assay.

81 Ca2+]i in response to E2 was determined in a fluorometric assay.

82 Cytokines were measured by multiplex fluorometric assay.

83 ical impedance spectroscopy (EIS) as well as fluorometric assay.

84 Total MMP activity was determined using a fluorometric assay.

85 y of caspase-3, -8, or -9 was measured using fluorometric assay.

86 transcription assays and (b) by quantitative fluorometric assays and direct microscopic observation o

87 Fluorometric assays and RT-PCR analysis of alpha1(I) col

88 Immunoblot analysis and fluorometric assays revealed that the extents of IR-indu

89 rthern analysis, gelatin-gel zymography, and fluorometric assays were performed on day 7 to determine

90 racellular Ca(2+) (iCa) concentrations using fluorometric assays, and blocked voltage-gated Ca(2+) ch

91 for performing quantitative colorimetric and fluorometric assays.

92 vices (muPADs) for performing ultrasensitive fluorometric assays.

93 erized by steady-state, presteady-state, and fluorometric assays.

94 Western blotting, immunohistochemistry, and fluorometric assays.

95 able to that of the best currently available fluorometric assays.

96 proliferation and chemotaxis, using specific fluorometric assays; and 3) gene expression, using pathw

97 Fluorometric assessments of electrochemically desorbed n

98 We also develop a high throughput compatible fluorometric-based assay for evaluating severity of dise

99 ssessed by measuring nitrate/nitrite using a fluorometric-based assay, iNOS expression was examined b

100 Using the novel fluorometric-based kinetic (real-time) RT-PCR, we determ

101 By traditional and fluorometric-based kinetic reverse transcription-PCR and

102 Here we present a fluorometric bioassay for TF-antigen (galactose-beta-(1-

103 A new approach for the design of a fluorometric biosensor for continuous monitoring of gluc

104 First, Fura-2 fluorometric Ca(2+) analysis shows the ability of PIP(3)

105 levels were determined by both a cell-based fluorometric Ca(2+) assay and a ratiometric Ca(2+) imagi

106 Based on fluorometric [Ca(2+)]i measurements, clemizole exhibits

107 isqualate, and 3,5-dihydroxyphenylglycine in fluorometric Ca2+ assays 3- to 6-fold, with EC50 values

108 gonist activity, with an IC50 of 3 microM in fluorometric Ca2+ assays, whereas the analog 3,3'-dichlo

109 In the fluorometric calcium assay, CDPPB exhibited an EC50 valu

110 Fluorometric calcium imaging and whole cell patch clamp

111 ion of whole-cell patch-clamp recordings and fluorometric calcium imaging, we characterized calcium t

112 Fluorometric calcium measurements also show that these d

113 In addition, fluorometric calcium measurements from retinal axon term

114 Fluorometric calcium measurements have revealed presynap

115 Fluorometric calcium measurements revealed that forskoli

116 Fluorometric calcium measurements revealed that this syn

117 lorimetric card was compared to the previous fluorometric card for identification of yeast.

118 An analysis of caspase activity, using fluorometric caspase assays and Western blots, indicated

119 entification of multiple targets in a single fluorometric channel based on fluorescent signal modulat

120 mongst targets that are measured in a single fluorometric channel.

121 s of honey samples were determined through a fluorometric-chemical characterization method and relate

122 f action was explored by spectrophotometric, fluorometric, chromatographic, immunometric, and cytoflu

123 ing for possible interactions between NM and fluorometric/colorimetric dyes and, most importantly, th

124 ity of NM altering the optical properties of fluorometric/colorimetric probes that are used to measur

125 Using a fluorometric coupled enzyme assay and smooth muscle myos

126 Ds) capped with thioglycolic acid (TGA) as a fluorometric Cyt c nanosensor.

127 describes the integration of laser-scanning fluorometric cytometry and nonseparation ligand-binding

128 The use of appropriate fluorometric derivatization procedures is of considerabl

129 nd quasi-quantitatively and allows for their fluorometric detection at low concentration.

130 to deployment in the developing world, where fluorometric detection is more problematic.

131 metric limit of detection of 10.8-191 nM and fluorometric detection limit from 0.25 to 121 nM in the

132 omolar concentrations in water, enabling the fluorometric detection of biologically relevant guests i

133 ed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved

134 nsor with regard to its applicability in the fluorometric detection of Cyt c.

135 ment and validation of a RP-HPLC method with fluorometric detection of derivatized isosteviol, formed

136 as been synthesized for the colorimetric and fluorometric detection of highly competitive H2S and cya

137 INOS activity was measured by fluorometric detection of NO.

138 for the colorimetric detection of Fe(2+) and fluorometric detection of Pb(2+) ions with high sensitiv

139 We find that fluorometric detection of RdRp activity is unreliable as

140 Rp inhibitors in biochemical assays based on fluorometric detection of RdRp activity or on the electr

141 e determination of these compounds, with the fluorometric detection providing substantially greater s

142 nce liquid chromatography (HPLC) method with fluorometric detection was developed for the routine det

143 Several methods for rapid sequestration, fluorometric detection, and the subsequent mass spectros

144 For fluorometric detection, the excitation and emission wave

145 labeled anti-phosphotyrosine antibody with a fluorometric detection.

146 ols, and lanthanides do not interfere in the fluorometric detection.

147 rocedure, based on enzymatic degradation and fluorometric detection.

148 fluorescently-labeled probe for single-plex fluorometric detection.

149 milar to the detection limit obtained with a fluorometric detector when using the CNTs.

150 neration of a fluorescent product allows for fluorometric determination of the conversion.

151 eport a graphene oxide (GO) nanosheets-based fluorometric DNA biosensor to study the type and locatio

152 es and approaches the sensitivity of typical fluorometric ELISAs.

153 hat obtained by using commercially available fluorometric-enzymatic assay and liquid chromatography/m

154 For fluorometric enzyme assay (FA) 1 (FA-1), 2'-(4-methylumb

155 An easy-to-perform fluorometric enzyme immunocapture assay (FEIA) was devel

156 ellular adhesion molecules were evaluated by fluorometric enzyme-linked immunosorbent assay.

157 mononucleotide and NAD in milk by means of a fluorometric, enzyme-coupled assay.

158 A shipboard fluorometric flow analyzer has been developed for near-r

159 With a fluorometric flow injection analysis system harnessed to

160 ed plants was monitored using histochemical, fluorometric GUS activity and fluorescence microscopy.

161 The fluorometric GUS analyses revealed that the promoter del

162 beled with 2-aminobenzamide, and analyzed by fluorometric, high-performance liquid chromatography (HP

163 Here we developed one type of fluorometric ICTS based on ultrabright semiconducting po

164 We developed and applied a high throughput Fluorometric Imaging Plate Reader (FLIPR) assay to monit

165 n to potentiate acetylcholine (ACh) in an M1 fluorometric imaging plate reader (FLIPR) functional ass

166 chieved using automated patch clamp versus a fluorometric imaging plate reader (FLIPR) in a high thro

167 An automated signaling assay device, the fluorometric imaging plate reader (FLIPR), can simultane

168 agonists were further characterized using a fluorometric imaging plate reader assay.

169 riments on rat basophilic leukemia cells and fluorometric imaging plate reader intracellular Ca(2+) a

170 Using a fluorescence-based fluorometric imaging plate reader membrane potential ass

171 Fluorometric imaging plate reader membrane potential dye

172 and rat recombinant P2X(7) cell lines using fluorometric imaging plate reader technology.

173 measurements of intracellular pH on a FLIPR (fluorometric imaging plate reader).

174 measurements of intracellular pH on a FLIPR (fluorometric imaging plate reader).

175 Herein, using a fluorometric imaging plate reader-supported Ca(2+) influ

176 n elevation of [Ca(2+)](i), measured using a fluorometric imaging plate reader.

177 Fluorometric imaging revealed that postsynaptic depolari

178 ed sample handling, and simultaneous 96-well fluorometric imaging to develop a high-throughput assay

179 Fluorometric imaging-based pharmacological characterizat

180 A simple fluorometric immunological method in combination with a

181 We used a simple fluorometric in vitro assay to determine clotting activi

182 The system was successfully applied to fluorometric (in-solution) and smartphone-assisted in-so

183 attonella marina has been examined using the fluorometric indicator alamarBlue.

184 ge (IC50 = 1.0-1.3 microM), as determined by fluorometric intracellular free Ca(2+) concentration ([C

185 ter classes require the determination of the fluorometric key performance parameter fluorescence quan

186 MP-9, MMP-12, and MMP-13 were assessed using fluorometric kits.

187 forts for developing the next generations of fluorometric lateral flow immunochromatographic strip (I

188 studied using enzymatic activity assays with fluorometric lipase and very low-density lipoprotein (VL

189 or fluorescence under UV lights (GFPUV) as a fluorometric marker and transformed Rickettsia typhi wit

190 ra-2 and mounted in superfusion chambers for fluorometric measurement of [Ca2+]i.

191 epithelial barrier function was assessed by fluorometric measurement of carboxyfluorescein uptake.

192 Tear clearance was assessed by fluorometric measurement of collected tear fluid 15 minu

193 and compared with NHE3 activity evaluated by fluorometric measurement of intracellular pH.

194 duces lipid headgroup order, as detected via fluorometric measurement of intramembrane electric field

195 reas higher sensitivities were obtained from fluorometric measurements down into sub-micromolar conce

196 he presence or absence of these agents using fluorometric measurements of intracellular Ca2+ concentr

197 Fluorometric measurements of iodide influx in Fischer ra

198 Fluorometric measurements on these cells using a fluores

199 re content was determined using quantitative fluorometric measurements.

200 rometry (r=0.630; n=853) and by an enzymatic-fluorometric method (triacylglycerol) (r=0.611; n=842).

201 samples were analysed for thiamine using the fluorometric method and minerals using ICP-MS.

202 The fluorometric method can also detect palladium bound to s

203 In addition, a less sensitive fluorometric method exists that employs nonpeptidyl amin

204 A fluorometric method for evaluation of activities of lipo

205 rformance liquid chromatography (HPLC)-based fluorometric method for measuring serine hydroxymethyltr

206 We developed a microplate-based fluorometric method for the concurrent determination of

207 The present paper describes an enzymatic-fluorometric method for the determination of cholesterol

208 A fluorometric method for the determination of hydroperoxi

209 We have developed a one-step fluorometric method for the measurement of monoamine oxi

210 An innovative fluorometric method has been developed to detect nitric

211 VICAM AflaTest and OchraTest immunoaffinity fluorometric method in a total of 50 meat products (25 e

212 Application of the immunoaffinity fluorometric method is an accurate, safe and rapid metho

213 The fluorometric method is based on the reaction of ammonium

214 This fluorometric method is based on the use of the fatty aci

215 ensively semiquantified by a catalysis-based fluorometric method that converts resorufin allyl ether

216 enzyme was used to develop a fixed endpoint fluorometric method to analyze UDP-GlcNAc.

217 Here, we report a fluorometric method to measure carboxypeptidase activiti

218 The fluorometric method using 2, 3-diaminonaphthalene as the

219 The fluorometric method utilized blue-emitting CDs (B-CDs),

220 A fluorometric method was utilized to determine the dissoc

221 on were used to assess H2O2 formation: (1) a fluorometric method with emission at 590 nm, (2) an opti

222 d the mutant enzymes were compared using the fluorometric method.

223 se data are comparable to that obtained by a fluorometric method.

224 tamine was quantified by a glass fiber-based fluorometric method; passive HR-IgE-stripped donor basop

225 hermal titration calorimetry, biosensors and fluorometric methods (including microscale thermophoresi

226 We discuss fluorometric methods for imaging or quantifying platinum

227 e report the development of colorimetric and fluorometric methods for the reliable quantitation of S-

228 2+ release and Ca2+ entry were measured with fluorometric methods in Chinese hamster ovary cells expr

229 plasma or serum with the use of colorimetric/fluorometric methods.

230 This review article discusses these fluorometric methods.

231 ed by up to 50%, as measured by quantitative fluorometric microscopy (QFM) of ezrin, indicating that

232 sociated fluorescence was quantified using a fluorometric microvolume assay technology (FMAT) scanner

233 escence-linked immunosorbent assay utilizing fluorometric microvolume assay technology (FMAT).

234 ogeneous bead-based immunoassay for use with fluorometric microvolume assay technology (FMAT).

235 glucose 6-phosphate into NADPH), followed by fluorometric NADPH detection.

236 Using the novel fluorometric NO detection system, 4,5-Diaminofluorescein

237 This allowed for the development of a fluorometric noncoupled assay that is 2 orders of magnit

238 In addition, the stability of the fluorometric oligonucleotides precludes the substrate va

239 le and quick carbon quantum dots(CQDs) based fluorometric "On-Off" probe was developed for detection

240 ease in muscle SIRT1 activity as measured by fluorometric or sirtuin activity assay.

241 g NH4(+)-OPA product was quantified by using fluorometric or spectrophotometric detection.

242 a trans full-length VP4 cleavage assay and a fluorometric peptide cleavage assay.

243 e 384-well and 1536-well microplates using a fluorometric plate reader for detection.

244 n be accomplished with a simple, inexpensive fluorometric plate reader.

245 (PPTES) and the click reaction to immobilize fluorometric probes (i.e., 3-azido-7-hydroxycoumarin, A-

246 e of caspase activation was determined using fluorometric profiling and the caspase inhibitor Z-Val-A

247 eTag molecules, which have the same fluorometric property, but distinct charge-to-mass ratio

248 re studied using western blot analysis and a fluorometric protease activity assay in the presence or

249 nhibition of protein splicing by zinc ion, a fluorometric protein splicing assay was developed in whi

250 n analysis by a factor of 50-100 relative to fluorometric qPCR readout.

251 Electrochemical and fluorometric quantification of a desorbed DNA probe sugg

252 -specific fluorescent label, fura-2, and its fluorometric quantification.

253 ding a low detection limit of 0.36 ng/mL for fluorometric quantification.

254 Fluorometric quantitation of EB was performed 1 or 2 h l

255 ures for the determination of other relevant fluorometric quantities including fluorescence quantum y

256 onvert AS-associated proteolytic activity to fluorometric readings enabling a sensitive and cost-effe

257 osensor was reported with a colorimetric and fluorometric readout system using graphene quantum dots

258 oximately five cycles compared to commercial fluorometric readout.

259 sed on this observation, we have developed a fluorometric, real-time assay that is adapted to a multi

260 Here, we used a fluorometric RNAP molecular beacon assay to discern part

261 f conducting simultaneous sweat sampling and fluorometric sensing of potential biomarkers, such as l-

262 tor (L1) exhibits selective colorimetric and fluorometric sensing of Zn(2+) in aqueous medium at pH 7

263 e, specific, and inexpensive nonenzyme-based fluorometric sensing platform as an alternative to conve

264 MOFs with widely varied fluorometric sensing properties have been developed usin

265 O(2) NPs), as a novel detection platform for fluorometric sensing.

266 ent and state of the art of colorimetric and fluorometric sensor arrays is presented.

267 ent and state of the art of colorimetric and fluorometric sensor arrays.

268 cohol), resulting in an advanced solid-state fluorometric sensor coating on a cellulose paper substra

269 sh-pull fluorophore systems in the design of fluorometric sensor materials due to the effect of intra

270 However, a sizable fraction of the fluorometric signals for VSDs I, II, and IV, but not VSD

271 The core of the sensing array is a novel fluorometric solid-state mechanism utilizing carbon poly

272 triphenyltetrazolium staining, colorimetric/fluorometric spectroscopy, and echocardiography, we foun

273 yzed using UV-visible spectrophotometric and fluorometric stopped-flow techniques.

274 Fluorometric studies demonstrated that LPS in vivo signi

275 Fluorometric studies demonstrated that LPS in vivo signi

276 Fluorometric studies link TNF's acid-enhanced membrane i

277 sterase 1 enzyme activity measured using the fluorometric substrate 4-methylumbelliferyl-6-thiopalmit

278 nts using an activity-based immunosassay and fluorometric substrate assay.

279 In experiments using fluorometric substrate-based lipase assays, there was a

280 Enzymatic analysis with fluorometric substrates showed that caspase 2, 3, and 9

281 A simple, sensitive and rapid fluorometric system has been developed for the detection

282 well with data obtained by a solution-phase fluorometric technique using a porous membrane diffusion

283 was quantified using an enzyme-coupled NADH fluorometric technique, regulatory myosin light chain (r

284 d its enzymatic activity was determined by a fluorometric technique.

285 osylated proteins were quantitated using the fluorometric technique.

286 dynamic data obtained using fluorimetric and fluorometric techniques in conjunction with fluorescence

287 cfDNA concentration was measured by a simple fluorometric test, at admission and for two consecutive

288 with various retinoids were characterized by fluorometric titration and photoaffinity labeling.

289 Fluorometric titration revealed that typical DNA stainin

290 in presence of Na(2)EDTA by both UV-Vis and fluorometric titration.

291 essions for analyzing spectrophotometric and fluorometric titrations are applicable to all fluorescen

292 ty distributions for Pb(II) binding, whereas fluorometric titrations are explained by monomodal distr

293 ty constants (K(obs)) measured by UV-vis and fluorometric titrations at variable pH for esters of 4,5

294 +) (K(d) = 0.3 micrometer), as determined by fluorometric titrations of the recombinant protein.

295 The methods employed are potentiometric and fluorometric titrations, fluorescence excitation-emissio

296 lues determined under the same conditions by fluorometric titrations.

297 Findings were confirmed with a fluorometric TRAP assay in which fluorescent primers spe

298 ed light microscopy and then the DeadEnd(TM) Fluorometric TUNEL System was used to observe nuclear DN

299 antly according to hemolysis grade; however, fluorometric values did.

300 We have devised a highly sensitive fluorometric well plate assay for tissue transglutaminas