ライフサイエンスコーパス: frameshifting

1 rtant role in biological functions including frameshifting.

2 ulatory and polyamine sensitizing effects on frameshifting.

3 shift site alone only supports low levels of frameshifting.

4 iple ribosomal translocation attempts during frameshifting.

5 low down mRNA translocation and promote mRNA frameshifting.

6 candidates for functional utilization of -1 frameshifting.

7 sing programmed ribosome pausing or inducing frameshifting.

8 here is no clear mechanistic description for frameshifting.

9 an function as a transactivator of ribosomal frameshifting.

10 out-of-frame pairing" model of translational frameshifting.

11 further influence miRNA processing and viral frameshifting.

12 genes inferred as sites of +1 translational frameshifting.

13 ue to PA-X, which was expressed by ribosomal frameshifting.

14 e how the m(1)G37 modification prevents mRNA frameshifting.

15 ding frame ("X-ORF"), accessed via ribosomal frameshifting.

16 t the stability of stem 1 is critical for -1 frameshifting.

17 e shorter gamma that arises by translational frameshifting.

18 extreme example of programmed translational frameshifting.

19 due, with CCU and CCC promoting efficient +1 frameshifting.

20 BWYV X-ray crystallography structure, in -1 frameshifting.

21 the shorter gamma, produced by translational frameshifting.

22 imit the fraction of ribosomes available for frameshifting.

23 s cautionary for other studies of programmed frameshifting.

24 sional resistance, and may thereby stimulate frameshifting.

25 lopment of anti-virus therapeutics targeting frameshifting.

26 ibosome biogenesis, and programmed ribosomal frameshifting.

27 codon position, gives a history of ribosome frameshifting.

28 translation errors and (5) mutations due to frameshifting.

29 ption errors, mRNA damage, and translational frameshifting.

30 zed that this modification was needed for -1 frameshifting.

31 tion, thereby inducing ribosomal pausing and frameshifting.

32 has some potential as a tool for studying -1 frameshifting.

33 ur pseudoknots cause -1 programmed ribosomal frameshifting.

34 as 'slippery' and promotes -1 translational frameshifting.

35 e shift site often act as cis-stimulators of frameshifting.

36 otifs significantly influences the levels of frameshifting.

37 egisters reminiscent of programmed ribosomal frameshifting.

38 truncated gamma that is created by ribosomal frameshifting.

39 tein sequences remain largely invariant upon frameshifting.

40 lower stem (LS) structure are important for frameshifting.

41 polypeptide chain that scales with observed frameshifting.

42 cipher the mechanism of programmed ribosomal frameshifting.

43 irus- or host-specific factors that modulate frameshifting.

44 the recoding site promote a precise level of frameshifting.

45 that are involved in programmed -1 ribosomal frameshifting (-1 PRF) are typically two-stemmed hairpin

46 Programmed -1 ribosomal frameshifting (-1 PRF) is a gene-expression mechanism us

47 Programmed -1 ribosomal frameshifting (-1 PRF) is a mechanism that directs elong

48 Programmed -1 ribosomal frameshifting (-1 PRF) is a widely used translational me

49 Programmed -1 ribosomal frameshifting (-1 PRF) is used by many positive-strand R

50 Programmed -1 ribosomal frameshifting (-1 PRF) stimulated by mRNA pseudoknots re

51 Many viruses utilize programmed -1 ribosomal frameshifting (-1 PRF) to express additional proteins or

52 WNV uses programmed -1 ribosomal frameshifting (-1 PRF) to synthesize the NS1' protein, a

53 alphaviruses utilize programmed -1 ribosomal frameshifting (-1 PRF) to synthesize the viral trans-fra

54 hese viruses utilize programmed -1 ribosomal frameshifting (-1 PRF) to synthesize the viral trans-fra

55 ch mechanism, termed -1 programmed ribosomal frameshifting (-1 PRF), to engineer ligand-responsive RN

56 ies have identified operational -1 ribosomal frameshifting (-1 RF) signals in eukaryotic genomic sequ

57 Programmed -1 ribosomal frameshifting (-1PRF) is a widely used translation recod

58 Programmed -1 ribosomal frameshifting (-1PRF) is tightly regulated by messenger

59 Programmed -1 ribosomal frameshifting (-1PRF) is used in various systems to expr

60 Recently, we described an unusual -2 frameshifting (-2 PRF) signal directing efficient expres

61 The -2/-1 programmed ribosomal frameshifting (-2/-1 PRF) mechanism in porcine reproduct

62 e diagnostic for +1 programmed translational frameshifting, a phenomenon disparately reported through

63 Nonstop stimulation of frameshifting also occurred when the C-chord was replace

64 we show that the induction of translational frameshifting also occurs under stressful conditions.

65 mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role

66 These data provide insight into retroviral frameshifting and could lead to alternative strategies t

67 nfluenza A virus shift site, triggers the +1 frameshifting and is enhanced by the increased propensit

68 ragenic SD-like sequences stimulate ribosome frameshifting and may also slow down ribosome movement a

69 non-slipped conformation, thereby preventing frameshifting and potentially enhancing DinB activity on

70 ctivity, stimulating programmed -1 ribosomal frameshifting and promoting virus propagation defects.

71 tential link between -1 programmed ribosomal frameshifting and response of a pseudoknot (PK) RNA to f

72 Frameshifting and splice site mutations were common, fou

73 ormational heterogeneity plays a key role in frameshifting and suggest that transitions between diffe

74 owed evidence of co-operative stimulation of frameshifting and the existence of multiple ribosome bin

75 which viruses use both programmed ribosomal frameshifting and translational attenuation to control t

76 nconventional initiation, but also ribosomal frameshifting and/or imperfect repeat DNA replication, e

77 unteracted by TraR antiactivation, ribosomal frameshifting, and FseA antiactivation.

78 reased leaky scanning through AUG codons, +1 frameshifting, and nonsense suppression.

79 yses demonstrated the induction of ribosomal frameshifting, and the generation and presentation of ab

80 Thus, as is also the case for ribosomal frameshifting, antiviral therapies targeting readthrough

81 ns suggest that small-molecule inhibitors of frameshifting are likely to have potential as agents for

82 e unfolding studies by optical tweezers, and frameshifting assays to elucidate how mechanical stabili

83 codon restriction to AUG and in restricting frameshifting at tandem ANN codons.

84 al antizyme requires programmed +1 ribosomal frameshifting at the 3' end of the first of two partiall

85 mutants in Salmonella enterica suggest that frameshifting at the end of pheL does not influence expr

86 is intrinsically susceptible to -1 ribosomal frameshifting at the sequence C-AAA-AAA.

87 provides parallels with programmed ribosomal frameshifting at the translation level.

88 Here we examined frameshifting at the U6A slippery sequence of the HIV ga

89 Ketolides promote frameshifting at the uORF, allowing the translating ribo

90 lippery sequence have also shown activity in frameshifting, at least in vitro.

91 Our results contradict a model of frameshifting based on structural rigidity and resistanc

92 s generated through programmed translational frameshifting, but the need for both forms is unclear.

93 t the genomic secondary structure attenuates frameshifting by affecting the overall rate of translati

94 f their key role in the control of ribosomal frameshifting by viral RNAs.

95 f studies using cell-free systems, ribosomal frameshifting can explain this ability to express TK.

96 dons before or after the G string argue that frameshifting can initiate within the first six guanines

97 tion of novel frameshift proteins, ribosomal frameshifting, coding sequence detection and the applica

98 Fits to a bursty model of frameshifting constrain frameshifting kinetic rates and

99 that genetic elements supporting productive frameshifting could rapidly evolve de novo, even in esse

100 sy and Williams-Beuren syndrome identified a frameshifting de novo variant in a major GABA(A)R gene,

101 novel missense mutation, R110W; and a novel frameshifting deletion, I298fsX307 in four families.

102 e consisted of three nonsense mutations, six frameshifting deletions, two frameshifting insertions, o

103 ity to force supports the hypothesis that -1 frameshifting depends on the difficulty of unfolding the

104 The degree of frameshifting depends on the nature of the sequence bein

105 The newly identified frameshifting determinants provide potential antiviral t

106 ms a similar task, although more slowly, for frameshifting DNA-protein alignments.

107 ormational plasticity of the high-efficiency frameshifting double mutant of the 26 nt potato leaf rol

108 n unusually high level, 15%, of +1 ribosomal frameshifting due to features of the nascent peptide seq

109 pes related to eEF2 function (i.e. increased frameshifting during protein translation and hypersensit

110 1) has an absolute requirement for ribosomal frameshifting during protein translation in order to pro

111 Ribosomal frameshifting during the translation of RNA is implicate

112 fication is essential for eEF2 to prevent -1 frameshifting during translation and show that the Gly(7

113 ism to influence the efficiency of ribosomal frameshifting during translation of viral RNA, indicatin

114 pairing at the ribosome A-site, and prevents frameshifting during translation.

115 ull-length TK polypeptide produced by net -1 frameshifting during translation.

116 al properties of a panel of pseudoknots with frameshifting efficiencies ranging from 2% to 30%: four

117 ng from 50 to 22 picoNewtons correlated with frameshifting efficiencies ranging from 53% to 0%.

118 tations were designed to generate a range of frameshifting efficiencies, yet with minimal impact on e

119 The biological significance of frameshifting efficiency and how the relative ratios of

120 nants of pseudoknot mechanical stability and frameshifting efficiency are not well understood.

121 ferent sites, but the factors that determine frameshifting efficiency are not yet fully understood.

122 mechanical stability of a pseudoknot and its frameshifting efficiency are regulated by tertiary stem-

123 interest in determining the extent to which frameshifting efficiency can be modulated before virus r

124 As a result, the frameshifting efficiency increases from 0 to 70% (one of

125 nt HIV strains to demonstrate that in cells, frameshifting efficiency is correlated with the stabilit

126 Previous work has suggested that frameshifting efficiency is related to the resistance of

127 derstanding of the molecular determinants of frameshifting efficiency may facilitate the development

128 nt mutations leading to a 3-fold decrease in frameshifting efficiency noticeably reduce virus replica

129 general translation, but also may alter the frameshifting efficiency of ribosomes, an event central

130 hese findings explain the unexpected drop in frameshifting efficiency to null levels of the C8U mutan

131 However, frameshifting efficiency was altered by stop codons down

132 However, increased frameshifting efficiency was correlated with an increase

133 al unfolding is not a primary determinant of frameshifting efficiency.

134 the transition state) could be correlated to frameshifting efficiency.

135 ity is identified as a determining factor in frameshifting efficiency.

136 rch, that encircles the programmed ribosomal frameshifting element.

137 The frameshifting elements comprise both a ribosomal slipper

138 Specifically, csoS2 was found to possess -1 frameshifting elements that lead to the production of th

139 Ribosomal frameshifting entails slippage of the translational mach

140 s the codon recognition patterns and reduces frameshifting errors during translation.

141 with the hypothesis of optimization against frameshifting errors in translation.

142 out the relationship between codon usage and frameshifting errors, an important form of processivity

143 f codon usage indicates optimization against frameshifting errors.

144 e feedback system in which the translational frameshifting event may be viewed in engineering terms a

145 lphavirus genomes suggested that a ribosomal frameshifting event occurs during translation of the alp

146 BUD22 affected the +1 translational frameshifting event required to express the Pol proteins

147 Correct annotation of a programmed frameshifting event requires manual evaluation.

148 ribe a novel, antibiotic-dependent ribosomal frameshifting event that activates translation of an ant

149 ed that they were characterized by ribosomal frameshifting events.

150 gously, a single smaller mRNA product with a frameshifting exclusion of B9D1 exon 4.

151 Depending on the signal, the frameshifting frequency can vary over a wide range, from

152 Frameshifting (FS) indels and nonsense (NS) variants dis

153 However, a frameshifting G insertion at virus passage 7 established

154 The mechanisms of frameshifting have been investigated in many systems, an

155 hanistic and conformational framework for -1 frameshifting, highlighting multiple kinetic branchpoint

156 Here we show that frameshifting in a model RNA virus, encephalomyocarditis

157 diverse and extensive usage of translational frameshifting in animal mitochondrial coding sequences.

158 ring translational stalling, which can alter frameshifting in both the stalled and trailing ribosomes

159 P- and A-sites toward promoting efficient +1 frameshifting in Escherichia coli.

160 ve investigated the history of programmed +1 frameshifting in fungi.

161 association with other cis elements, promote frameshifting in human mitoribosomes.

162 To investigate frameshifting in infected cells, we constructed viruses

163 rus virulence protein generated by ribosomal frameshifting in segment 3 of influenza virus coding for

164 Programmed frameshifting in the RF2 gene (prfB) involves an intrage

165 oknot structures in messenger RNAs stimulate frameshifting in upstream slippery sequences.

166 structure is sufficient to promote efficient frameshifting in vitro.

167 a novel GFP-based method to monitor antizyme frameshifting in vivo, we show that the induction of tra

168 identified promoted significant levels of +1 frameshifting in vivo.

169 human homolog of copA, and direct ribosomal frameshifting in vivo.

170 We also show that although the first frameshifting indel in a gene causes loss of function, t

171 function, there is a tendency for the second frameshifting indel to compensate and restore protein fu

172 The percentage of human frameshifting indels predicted to be gene-damaging is ne

173 created SIFT Indel, a prediction method for frameshifting indels that has 84% accuracy.

174 Each human has approximately 50 to 280 frameshifting indels, yet their implications are unknown

175 hogenicity and the functional effects of non-frameshifting insertion/deletion variants.

176 types of functional residues impacted by non-frameshifting insertion/deletion variation.

177 Among different types of variation, non-frameshifting insertions and deletions (indels) represen

178 mutations, six frameshifting deletions, two frameshifting insertions, one missense (Leu348Arg) mutat

179 Translational frameshifting involves the repositioning of ribosomes on

180 Programmed -1 ribosomal frameshifting is a mechanism of gene expression, whereby

181 Programmed ribosomal -1 frameshifting is a non-standard decoding process occurri

182 The high efficiency of frameshifting is achieved by the combined stimulatory ac

183 ere we show that the the trans-activation of frameshifting is carried out by a protein complex compos

184 finding of incidental, rather than utilized, frameshifting is cautionary for other studies of program

185 s, the efficiency of programmed -1 ribosomal frameshifting is critical for ensuring the proper ratios

186 Programmed -1 ribosomal frameshifting is employed in the expression of a number

187 Thus, frameshifting is essential for viral replication.

188 In RSV, frameshifting is essential in the production of the Gag-

189 Frameshifting is induced by mRNA secondary structures th

190 The detailed biophysical mechanism by which frameshifting is induced remains unknown.

191 Together with dynamic codon redefinition, frameshifting is one of the forms of recoding that enric

192 the level of full-length TK, indicating that frameshifting is strongly stimulated by a new mechanism,

193 polyamine-independent regulation of antizyme frameshifting is suggested.

194 ly, a major distinctive rule of bacterial -1 frameshifting is that the most efficient motifs are thos

195 Frameshifting is typically stimulated by signals contain

196 Ribosomal frameshifting is utilized for the synthesis of additiona

197 In contrast, a 3-fold stimulation of frameshifting is well tolerated.

198 Programmed -1 ribosomal frameshifting is widely used in the expression of RNA vi

199 ppage site, which is important for ribosomal frameshifting, is shown here to limit reverse transcript

200 to a bursty model of frameshifting constrain frameshifting kinetic rates and demonstrate how ribosoma

201 has uncovered many details about single RNA frameshifting kinetics in vitro, little is known about h

202 ested whether pseudoknots bound with an anti-frameshifting ligand exhibited a similar correlation bet

203 We show that frameshifting may also be deliberately induced by chemic

204 Our results suggest that frameshifting may be a powerful evolutionary mechanism f

205 Moreover, protein-induced transactivation of frameshifting may be a widely used mechanism, potentiall

206 These results suggest that suppression of frameshifting may be needed in the absence of an active

207 d that an evolutionarily conserved ribosomal frameshifting mechanism is used by simarteriviruses and

208 s expressed via a novel programmed ribosomal frameshifting mechanism.

209 eriments may be a significant feature of the frameshifting mechanism.

210 These compounds are able to enhance frameshifting more than 50% in a dual-luciferase assay i

211 The frameshifting mRNA (FSmRNA) contained the frameshifting

212 This individual carrying the RYR1 frameshifting mutation complained of mild muscle weaknes

213 igation of an apparent correlation between a frameshifting mutation in the canonical first exon of NO

214 Such frameshifting normally occurs at a set ratio and is util

215 A basal level of frameshifting occurring in the absence of the RSE increa

216 -1 frameshifting occurs on Escherichia coli's dnaX mRNA con

217 Mutational studies indicated that frameshifting occurs on or near the C-chord, a region la

218 Ribosomal frameshifting occurs when a ribosome slips a few nucleot

219 ddition, we determined that bL9 can suppress frameshifting of its host ribosome, likely by regulating

220 One class of regulatory frameshifting of stable chromosomal genes governs cellul

221 rior to extrusion of the target cytosine and frameshifting of the DNA recognition sequence.

222 an mRNA secondary structure that promotes -1 frameshifting on a homopolymeric slippery sequence.

223 ht function as trans-acting switches to turn frameshifting on or off in response to cellular conditio

224 he absence of m(1)G37 in tRNA(Pro) causes +1 frameshifting on polynucleotide, slippery codons.

225 vely as a Gag-Pol fusion either by ribosomal frameshifting or by read-through of the gag stop codon.

226 c RNA viruses and retroviruses use ribosomal frameshifting or stop codon readthrough to regulate expr

227 By examining -1 and -2 frameshifting outcomes on mRNAs with varying slippery se

228 a novel viral protein expressed by ribosomal frameshifting, PA-X, was found to play a major role in i

229 RNA viruses for translational readthrough or frameshifting past termination codons for the synthesis

230 attenuator element does not actually affect frameshifting per se but rather serves to limit the frac

231 Frameshifting permits expression of more than one polype

232 us (SARS-CoV) employ programmed -1 ribosomal frameshifting (PRF) for their protein expression.

233 Programmed ribosomal frameshifting (PRF) is a conserved translational recodin

234 Programmed -1 ribosomal frameshifting (PRF) is a distinctive mode of gene expres

235 Programmed ribosomal frameshifting (PRF) is a mechanism used by arteriviruses

236 Programmed ribosomal frameshifting (PRF) is a process by which ribosomes prod

237 ational control through programmed ribosomal frameshifting (PRF) is exploited widely by viruses and i

238 n of reads flanking the programmed ribosomal frameshifting (PRF) signal at the genomic RNA ORF1a/ORF1

239 tivating a unique -2/-1 programmed ribosomal frameshifting (PRF) signal for the expression of framesh

240 In +1 programmed ribosomal frameshifting (PRF), ribosomes skip one nucleotide towar

241 l mechanisms, including programmed ribosomal frameshifting (PRF), which facilitates the production of

242 s in mRNA can stimulate programmed ribosomal frameshifting (PRF).

243 Here, we reveal hidden aspects of the frameshifting process, including its exact location on t

244 the translating ribosome interferes with the frameshifting process.

245 Programmed ribosomal frameshifting produces alternative proteins from a singl

246 eshifting (PRF) signal for the expression of frameshifting products.

247 Programmed ribosomal frameshifting provides a mechanism to decode information

248 Here we describe the unfolding of the frameshifting pseudoknot from infectious bronchitis viru

249 chanical unfolding and refolding of the four frameshifting pseudoknots.

250 le Mig-7 mRNA secondary structures may cause frameshifting, read-through, and/or recoding of the mult

251 Programmed frameshifting (recoding) to generate multiple proteins f

252 crobe, Chamanian et al. (2013) show that the frameshifting region in the HIV-1 genome influences the

253 bined with RNA-binding assays and cell-based frameshifting reporter assays reveal a number of key res

254 tide in its anticodon loop that undergoes +1 frameshifting, reveal that m(1)G37 destabilizes interact

255 g RNA are translated at similar rates as non-frameshifting RNA (~3 aa/s) and can continuously framesh

256 Frameshifting RNA are translated at similar rates as non

257 nd-slip model of frameshifting, we developed Frameshifting Robustness Score (FRS).

258 ely 1.9 s(-1) for the release factor 2 (RF2) frameshifting sequence.

259 Similar observations for a frameshifting signal indicate that this novel equilibriu

260 Frameshifting signals comprise a heptanucleotide slipper

261 he frameshifting mRNA (FSmRNA) contained the frameshifting signals: a Shine-Dalgarno sequence, a slip

262 erichia coli dnaX gene, which contains three frameshifting signals: a slippery sequence (A AAA AAG),

263 sed premature translation termination at the frameshifting site.

264 uggesting no functional significance for the frameshifting sites.

265 ic jams contribute to the persistence of the frameshifting state.

266 These results indicate that frameshifting takes place during the repetitive ribosoma

267 ibosomes translating copA undergo programmed frameshifting, terminate translation in the -1 frame, an

268 One facet of this is frameshifting that often results in synthesis of a C-ter

269 Furthermore, frameshifting the gene in the first coding exon with a s

270 he over-reading of stop codons via ribosomal frameshifting, the existence of an antizyme and an antiz

271 r translation regulation, such as programmed frameshifting, the modulation of protein expression leve

272 In many cases of productively utilized frameshifting, the proportion of ribosomes that frameshi

273 Many viruses use programmed -1 ribosomal frameshifting to express defined ratios of structural an

274 highly efficient +1/-2 programmed ribosomal frameshifting to generate previously undescribed alterna

275 RNAs) contain motifs that promote deliberate frameshifting to regulate production of the encoded prot

276 icate polyA runs cause ribosome stalling and frameshifting, triggering mRNA surveillance pathways and

277 In addition to confirming that clustered -1 frameshifting variants in DVL1 and DVL3 are the main con

278 The efficiency of frameshifting varies widely for different sites, but the

279 along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understoo

280 The genomic organizations of many other frameshifting viruses, including the coronaviruses, are

281 Because frameshifting was also prevalent in a polyamine auxotrop

282 tingly, during starvation, the initiation of frameshifting was independent of polyamine concentration

283 Moreover, +1 frameshifting was not suppressed by tmRNA.SmpB activity,

284 tory RNA spacing distances, we found that -2 frameshifting was optimal at a spacer length 1-2 nucleot

285 in vitro RNA dimerization was abolished, and frameshifting was reduced from 15 to 5.7%.

286 The efficiency of frameshifting was relatively high, 3-5%, as the polypept

287 e -1 PRF strongly promote this activity, but frameshifting was significantly more efficient upon incl

288 sites and to help elucidate the mechanism of frameshifting, we determined eight new complete or nearl

289 he recently proposed pause-and-slip model of frameshifting, we developed Frameshifting Robustness Sco

290 e role of SD-ASD pairing in the mechanism of frameshifting, we have analysed the effect of spacing be

291 es that trigger genuine programmed ribosomal frameshifting; we have experimentally confirmed four new

292 specific Abs, and the site and direction of frameshifting were determined via mass spectrometric ana

293 these RNA structures to induce +1 ribosomal frameshifting when annealed downstream of the frameshift

294 Programmed -1 frameshifting, whereby the reading frame of a ribosome o

295 We propose that mRNA tension is central to frameshifting, whether promoted by stem-loop, pseudoknot

296 ippage is the driving force for +1 ribosomal frameshifting while the presence of a 'hungry codon' in

297 nucleotides shorter than that optimal for -1 frameshifting with all stimulatory RNAs tested.

298 gnal and found high levels of both -1 and -2 frameshifting with stem-loop, pseudoknot or antisense ol

299 tem, hypomodification increased Phe-specific frameshifting, with incremental changes in frameshift ef

300 t the nascent peptide level to stimulate the frameshifting, without involving stalling detectable by