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1 tive to that of the parent complex of strain-free ligand.
2 e derived by neglecting the concentration of free ligand.
3 the separation of antibody-bound ligand from free ligand.
4 rom the solid state (X-ray) structure of the free ligand.
5  1500 and 1220 cm(-1) that are absent in the free ligand.
6 enefit provided by the use of a nuclear spin-free ligand.
7 receptor-fluorescent probe complexes but not free ligand.
8 imulations on this complex as well as on the free ligand.
9 acrylamide was barely different from that of free ligand.
10 FB)+ complex upon administration of the iron-free ligand.
11 st to the fast fluorescence quenching in the free ligand.
12 rences in the solvation free energies of the free ligands.
13  separation of a protein-ligand complex from free ligands.
14  a greater extent than an equivalent dose of free ligands.
15  had higher affinity to DNA than their metal-free ligands.
16 xazaboronin-6-yl)benzene (4), as well as the free ligand 2-[[(3,5-di-tert-butyl-2-hydroxyphenyl)imino
17   In the electrostatic potential maps of the free ligands, a higher positive charge is present in the
18 orption spectrum, leading to decrease of the free ligand absorption and increase of complex absorptio
19                              The decrease of free ligand absorption exposes emission of the transduce
20 ries are separated from one another and from free ligand according to their electrophoretic mobilitie
21 wn to occur by a similar process to generate free ligand and a bis-ligand complex formed by reaction
22 erophore, deferoxamine mesylate, in both the free ligand and Fe-bound forms.
23 pable of resolving spectral responses of the free ligand and its acid-base forms along the monomeric
24 e spectroscopy in water and D(2)O, using the free ligand and wild-type and double-mutant enzyme.ligan
25   The distribution coefficient values of the free ligands and corresponding iron(III) complexes betwe
26 tent than their 3-methoxy analogs, while the free ligands and metal(III) ions showed little or no cyt
27 s associated with metal complexes along with free ligands and other related adducts in high-resolutio
28 d provides better contrast between bound and free ligands and requires a protein-ligand ratio ca. 25
29                      The pK(a) values of the free ligands and the affinity constants of their iron co
30 d line shape analysis were performed for the free ligands and the complexes.
31 chemical and photophysical properties of the free ligands and their copper complexes were investigate
32 irical values of mobilities of free protein, free ligand, and electroosmotic flow.
33                            When nuclear spin-free ligands are employed, vanadium(IV) complexes can sh
34         While the chiroptical spectra of the free ligands are unremarkable, the CD spectra of the com
35 he electropherograms resolved and quantified free ligand as well as 1:1 and 2:1 SOMAmer-CRP complexes
36 al transduction by CARs, we developed a cell-free, ligand-based activation and ex vivo culture system
37 50-kDa complex to a 150-kDa complex and to a free ligand, because the binding of heparin with IGFBPs
38  The bound formate can be distinguished from free ligand by the binding-induced sharpening and downsh
39 extract the binding density as a function of free ligand concentration eluting from the cell.
40 regation phenomenon to directly quantify the free ligand concentration in equilibrated bacteria-ligan
41 egative) often relies on the assumption that free ligand concentration, L, can be approximated by the
42 not its linkage isomers, adopts an organized free-ligand conformation that resembles the STING-bound
43     Our results demonstrate that analyses of free-ligand conformations can be as important as analyse
44       Nuclear Magnetic Resonance (NMR)-based free ligand conformer analysis demonstrates that such un
45    Binding was abolished by competition with free ligands, demonstrating the validity of the affinity
46 VDR on a naked DNA template utilizing a cell-free, ligand-dependent transcription assay.
47                          Here we present the free-, ligand (dGTP)- and inhibitor (GTP)-bound structur
48  for folding, as NMR and X-ray show that the free ligands do not adopt helical structures.
49  report a vanadium complex in a nuclear-spin free ligand field that displays two key properties for a
50 s complexed with a target are separated from free ligands) for a target in a mixture gives a greatly
51   Furthermore, we explain the effect of high free ligand fraction on the early time dynamics of spin
52 y (only E,E-isomer) was achieved from a base-free, ligand-free, and mild catalytic condition with a l
53 P-Te bond constructions occurred under metal-free, ligand-free, oxidant-free, and photocatalyst-free
54                                        Crack-free, ligand-free, phase-pure nanostructured solids, usi
55 ple draining device for batch decantation of free ligand from 96 minitubes is used.
56 bility of dry nitrocellulose to separate the free ligand from bound protein-ligand complexes.
57  they facilitated good-quality separation of free ligands from the protein-ligand complexes.
58 t more effective than FeIII(HDFB)+, the iron-free ligand H4DFB+ was significantly more effective than
59                                          The free ligand has been prepared, showing much higher stabi
60 e results of which conclusively rejected the free ligand hypothesis.
61 linical safety studies contains a mixture of free ligand (i.e., ligand-linker-chelator without metal)
62 dissociated in the mass spectrometer and the freed ligands identified.
63  we demonstrate that the presence of unbound/free ligand in colloidal suspension plays a pivotal role
64  stages that can be computed separately: the free ligand in the bulk is first restrained into the con
65                   Unexpectedly, dGal-1 bound free ligands in solution with relatively low affinity an
66 ral orders of magnitude in comparison to the free ligands in solution.
67 ability of the possible monomers because all free ligands in the solution were consumed by the side r
68 antibacterial activity relative to the metal-free ligand (in the absence of any adjuvants), thereby d
69  rates are dependent on the concentration of free ligand, indicating the potential for direct transfe
70 ably reflects the effect of constraining the free ligand into the conformation required for binding,
71 d ligand at the site of application, whereas free ligand is mobilized by bulk movement of the solvent
72  quantum yield ratio of monozinc complex and free ligand) is qualitatively related to the highest occ
73 arison with MD simulations performed for the free ligand, it has been determined that GS-1-B(4) recog
74 re dependence for the response curves of the free ligand (L), native apo-protein (N), native ligand-b
75                   The protonated form of the free ligand (lambda(max) = 336 nm) yields the correspond
76 ds, which lead to bathochromic shifts of the free ligands, lead to similar bathochromic shifts in the
77 r of magnitude affinity enhancement over the free ligand-lectin interactions was observed which can b
78 f H2 to 2 was carried out in the presence of free ligand Mes3SnH (Mes = 2,4,6-Me3C6H2).
79 g for clinical use, where only a fraction of free ligand molecules chelate the radioactive metal to f
80 ceptive potential of these complexes and the free ligands (noncoordinated to ruthenium) was tested in
81 lenges include poor assay sensitivity of the free ligand NVS001, formation of the free ligand (NVS001
82 eloped for the simultaneous determination of free ligand (NVS001) and cold ligand ((175)Lu-NVS001) in
83  of the free ligand NVS001, formation of the free ligand (NVS001) with endogenous metal (e.g., potass
84 t depend on rapid exchange between bound and free ligand or on stable isotope labeling, relying inste
85 by the presence of sugars, high salt levels, free ligands, or detergents.
86  addition of excess In(III) to recomplex all free ligands, previously added DTPA as well as the ligan
87                                  Whereas the free ligands show a preference for the same enantiomer o
88 longer diffusion times also selectively edit free ligand signal, the measured diffusion coefficients
89 uilibrium concentration distributions of the free ligand species and the corresponding microscopic li
90 variable and the concentration of a bound or free ligand species as dependent variable.
91 se models with parameters derived from metal-free ligand structures should make this approach scalabl
92 igand does not need to be separated from the free ligand thus avoiding vacuum filtration and (ii) the
93 h factor Xa protease, and by the addition of free ligand to the infection.
94 es of 6-60 muM for three unsubstituted metal-free ligands, whereas values for the metal complexes var
95  decrease to the absence of one nuclear spin-free ligand, which served to shield the vanadium centers
96 wer rates, hydride 20 reductively eliminates free ligand, which ultimately generates a bis-ligand com
97 d a bis-ligand complex formed by reaction of free ligand with an active catalyst species.