ライフサイエンスコーパス: frozen

1 underwent liquid culture, and an aliquot was frozen.

2 sive motion of a subset of ribosomes is also frozen.

3 ized in acetonitrile, and the homogenate was frozen.

4 ich the overlying ground was not permanently frozen.

5 ese three compounds were prepared and stored frozen (150 days, -12 degrees C).

6 ample was divided into four parts: (1) fresh-frozen, (2) direct-fixed in formalin for 18 h, (3) froze

7 ferentially expressed genes (DEGs) vs. fresh-frozen, 98% of which were down-regulated.

8 exploration during obstruction describing a "frozen abdomen." All 5 responded at least temporarily to

9 ode likely results from the combination of a frozen accident, i.e., the deleterious effect of codon r

10 (2) fresh mouse brain tissue, and (3) human frozen adipose tissue (AT) from six individuals.

11 ltiple cryo-EM reconstructions of the enzyme frozen after the addition of MgATP to identify the chang

12 ces in superfluid (4)He, using submicrometer frozen air tracers.

13 this was the only form in 93 of 103 (90.3%) frozen and all 10 fresh samples tested using DNase-CMV-q

14 ran 4 the conformational equilibria could be frozen and assigned.

15 pecimens, including paraffin-embedded, fresh frozen and chemically stained human tissues.

16 at the recombinant protein is stable at both frozen and elevated-temperature storage conditions.

17 NanoCRCA classified fresh frozen and FFPE samples into all five CRCA subtypes with

18 supported by retrospective studies of paired frozen and FFPE samples, which identified consistent enr

19 s in RNA-sequencing profiles between matched frozen and FFPE samples.

20 cing and small RNA profiling on matched snap-frozen and FFPE specimens exposed to different delays to

21 set of RNAs was found to differ between snap-frozen and FFPE specimens in a consistent manner across

22 ketoglutarate, malic acid, and glutamate) in frozen and FFPE tissues specimens.

23 ination of 2HG enantiomers (D- and L-2HG) in frozen and FFPE tissues.

24 nce for SRH images contrasted to H&E-stained frozen and formalin-fixed paraffin-embedded (FFPE) tissu

25 ol; each lab tested blinded batches of three frozen and one fresh sample.

26 ngle-use aliquots of the supernatants can be frozen and stored (-20 degrees C; 12 months).

27 In this test, wine samples are frozen and then thawed in controlled conditions.

28 HRs with 95% CIs for yogurt (nonfrozen or frozen) and other dairy consumption in relation to incid

29 d type of stools used (for example, fresh or frozen), and duration of stool conservation (67%).

30 control measures before testing fresh, flash frozen, and cryopreserved breast cells and tissue.

31 which a whole mouse brain is embedded, flash frozen, and cryosectioned in preparation for mass spectr

32 were euthanized, the hearts were extracted, frozen, and cut into 20-mum short axis slices.

33 es that are challenging to dissociate or are frozen, and opens the way to human genetics studies, cli

34 Thousands of frozen, archived tissue samples from the human central n

35 C as determined by DSC: 25% of water is not frozen at -20 degrees C, possibly due to anti-freezing p

36 Nasal swabs sampled and frozen at ICU admission were tested using a multiplex PC

37 pes of baits were used: (1) frozen bait; (2) frozen bait and natural chum; (3) fresh fish bait; and (

38 iolent interactions with the bait, while the frozen bait did not generate a defined behavioural patte

39 Four types of baits were used: (1) frozen bait; (2) frozen bait and natural chum; (3) fresh

40 we show that the subtle random potential of frozen BCO Brownian rotors suppresses magnetic order dow

41 ed the drip loss and electrolytic leakage in frozen beans, revealing that it preserved the membrane i

42 -MS was performed to evaluate the quality of frozen beans.

43 In polystyrene glass and in frozen benzene or dibutyl phthalate solution, both 1 and

44 rus outbreak due to commercially distributed frozen berries identified in the United States.

45 of precursors (up to 19-fold) in juices from frozen berries.

46 critical for calibrating the depth scale of frozen biological samples.

47 mission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit f

48 s and pediatric participants that used fresh frozen biopsies.

49 The increased risk of pre-eclampsia after frozen blastocyst transfer warrants further studies.

50 For those assigned to frozen blastocyst transfer, all blastocysts were cryopre

51 The crossover treatments were frozen breakfast meals containing 100 g of liquid (~2) w

52 Samples of frozen breasts from chickens slaughtered at 42 days of a

53 Frozen breasts samples were studied before and after ref

54 sted at least 80 of 100 oral aFMT or placebo frozen capsules during the transplantation period.

55 as permafrost thaw exposes immense stores of frozen carbon (C) to microbial decomposition.

56 radation and the decomposition of previously frozen carbon.

57 estimating assay precision and the effect of frozen cell storage on estimated reservoir size.

58 the mouse, starting from freshly isolated or frozen cell suspensions.

59 e dependent behaviour during phase change of frozen cell therapies and its effect on live cell suspen

60 thaw suggesting mechanical disruption of the frozen cells.

61 -thin regions (called lamella) in the intact frozen cells.

62 illed block-face EM across entire vitreously frozen cells.

63 yopreserving and regenerating a species from frozen cellular material are currently technically unfea

64 a systematic toolbox for profiling fresh and frozen clinical tumor samples using scRNA-Seq and snRNA-

65 severely restricted protein motion under the frozen conditions, which highlights the remarkable confo

66 nce with different thermal histories (fresh, frozen, cooked) as affected by pH, water and NaCl additi

67 Fresh and frozen corneal donors offer similar clinical outcomes wh

68 Ten frozen cuttlefish samples did not reveal any treatment,

69 (25 CIS and 5 muscle controls) from 26 fresh-frozen cystectomy specimens.

70 ding propensity in podocytes resulting in a 'frozen' cytoskeleton.

71 For validation, analysis of frozen day 14 UUO kidney revealed rare juxtaglomerular c

72 The down-regulation of proteins in frozen durian pulps indicated that frozen storage has af

73 r 100 000 for children born after the use of frozen embryo transfer (n = 3356).

74 ytoplasmic sperm injection [n = 13 417], and frozen embryo transfer [n = 3356]).

75 ezing all embryos and undertaking a deferred frozen embryo transfer might increase pregnancy rate aft

76 h children born to fertile women, the use of frozen embryo transfer was associated with an elevated r

77 Among children born in Denmark, the use of frozen embryo transfer, compared with children born to f

78 e women were undergoing IVF (fresh-embryo or frozen-embryo transfer), with no recent exposure to disr

79 ferring to the system without coupling to a "frozen" environment, and 1 referring to the system's com

80 based lipoprotein profiling was performed on frozen fasting plasma obtained at catheterization.

81 mples; 25 of these had direct FFPE vs. fresh frozen (FF) replicates, 57 were sequenced in 2 different

82 lin-fixed paraffin embedded (FFPE) and fresh frozen (FF) sequential patient-matched breast tumour sam

83 DNA isolated from DFPE and fresh frozen (FF) tissues were sequenced by NGS.

84 d in Barcelona, Spain and included 112 fresh frozen (FF, median age 69, 44% women) and 163 formalin-f

85 ent classification of selected matched fresh frozen/FFPE samples.

86 lly saturated with injection fluid, S = 1; a frozen finger region, r(b) < r < r(f) (t), in which the

87 ride-containing raw materials (e.g., breaded frozen fish products) can lead to the formation of fatty

88 ling approach is particularly timely for the frozen food industry, seeking science-based recommendati

89 Broccoli by-products from frozen-food industry account for 45% of the initial broc

90 y, cryopreservation of cells and organs, and frozen foods.

91 tion efficiency is maintained when cells are frozen for long term storage.

92 dria were used for real-time respirometry or frozen for protein content and activity assays.

93 Aliquots were frozen for testing on different diagnostic platforms, as

94 Fresh stool collections were flash frozen from 236 infants at <=6 time points.

95 exists that involves sequential advection of frozen fronts from the center of the droplet to its boun

96 udy aimed to determine pesticide residues in frozen fruit and vegetables from Chilean retail stores u

97 yphenols, vitamin C and folates in fresh and frozen fruits using HPLC-UV analyses.

98 by the presence of partially molten sills or frozen gabbroic sills.

99 T (gm) , the configurational entropy of the frozen glass continues to fall rapidly, approaching that

100 sical studies of the isolated molecules in a frozen glass, in combination with DFT/MRCI calculations,

101 d lifetimes was assessed in fluid solutions, frozen glassy matrices, amorphous solids, and crystallin

102 10-fold) occurred in the wines derived from frozen grapes, with parallel increases of precursors (up

103 The sensitivity of permanently frozen ground (permafrost) in the Northern Hemisphere to

104 count per cell of 29,201 from both fresh and frozen hippocampi, observing little difference in access

105 successfully conduct 3D molecular imaging of frozen homogenized milk and observe network structures o

106 microvessel preparations isolated from snap-frozen human and mouse tissues by laser capture microdis

107 ranscripts in a cell-type specific manner in frozen human brain tissue using multiplexed fluorescent

108 Fresh and frozen human heart tissue was used.

109 ry, our optimised method allows the usage of frozen human samples from brain banks to perform quantit

110 we attempted to identify senescent cells in frozen human skeletal muscle biopsies using these marker

111 lation of nuclei of specific cell types from frozen human tissue, followed by bulk RNA-Sequencing.

112 e particles from low-contrast micrographs of frozen hydrated biomolecules by cryo-electron microscopy

113 X-ray holographic nanotomography on "thick" frozen-hydrated cells.

114 By cryo-electron tomography of frozen-hydrated endothelial cells, we identify internal

115 er show that the method can be used to study frozen-hydrated specimens of rotavirus double-layered pa

116 mples such as intact cells in a near-native, frozen-hydrated state to macromolecular resolution ( app

117 We discover that analysis in the frozen-hydrated state yields a 10 000-fold increase in s

118 A bullet containing the frozen, hyperpolarized sample is ejected using pressuriz

119 Frozen ice packs were then affixed over the right PG of

120 Here, we developed FIN-Seq (Frozen Immunolabeled Nuclei Sequencing), a method that a

121 and spleens dissected, and remaining tissues frozen in situ for later analyses.

122 conformations of "linear" ema(4-) to ema(4-) frozen in the "tight-loop" around single metals, and to

123 y of several trophically interacting taxa is frozen in time by exceptional preservation.

124 Sturgeons seem to be frozen in time.

125 Rayleigh scattering, arising from frozen-in density fluctuations in the glass, fundamental

126 to the mantle due to asthenospheric flow or frozen-in features of the lithosphere.

127 NPAs were frozen; IS underwent liquid culture, and an aliquot was

128 In comparison to wines from fresh grapes or frozen juices, significant elevation of varietal thiols

129 We used remnant frozen kidney-biopsy specimens from 34 patients with IgA

130 ead fractions likely consist of mostly newly frozen leads that reduce any pre-existing low-level clou

131 We find that newly frozen leads with large sensible heat flux but low laten

132 and protein oxidative stability of long-term frozen lean beef produced using stepwise in-bag ageing p

133 ivity to humidity, and the stress induced in frozen liquids inhibit ILs from constituting an ideal pl

134 iated by accidental exposure of livestock to frozen material contaminated with virus from approximate

135 enome and reconstitute an avian species from frozen material.

136 lute concentrations in the unfrozen water of frozen meat is the primary cause of myofibrillar protein

137 In contrast, if these reflections were frozen melt sills, they would imply the presence of thic

138 le-genome sequencing (WGS) analysis of fresh-frozen metastatic biopsies from 197 mCRPC patients.

139 ortem plasma-derived cell-free DNA and eight frozen metastatic cancer tissues collected during rapid

140 experiments were terminated, cell lines were frozen, mouse colonies were culled, and trainees were pr

141 expression analyses to discover that neither frozen, nor fresh hAFSCs cultivated in multipotent stem

142 Probe-Seq is compatible with frozen nuclei, making cell types within archival tissue

143 ompletely avoided when the complex is plunge-frozen on a substrate of hydrophilized graphene.

144 states, while nuclear motion is essentially frozen on this timescale.

145 fecal sample that was processed into aFMT by frozen, opaque, and odorless capsules.

146 iline concentrations for the AP method, BMD (frozen or dry plates), and MGIT960, respectively.

147 eus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors.

148 th-of-field (DOF), resected tissue is either frozen or preserved with chemical fixatives, sliced into

149 Existing methods, developed based on fresh-frozen or similar-type samples, may cause suboptimal per

150 re expected to increase as massive stocks of frozen organic matter in permafrost are liberated in a w

151 We performed microRNA sequencing in 74 frozen osteosarcoma biopsy samples, constituting the lar

152 Frozen ovarian tissue showed higher ROS levels and lower

153 examined using in situ hybridization on the frozen pancreatic sections.

154 onocytes, and CD3 + T cells were sorted from frozen peripheral blood mononuclear cells from 11 RV-inf

155 Frozen peripheral blood mononuclear cells of 30 human im

156 t shows that DOM originating from previously frozen permafrost peatlands is highly aromatic and previ

157 Frozen permafrost Pleistocene mammal carcasses with soft

158 Ultrastructural observations of frozen PG oocytes showed rupture of the plasma membrane,

159 albumin dissolvent (68.1%) compared to fresh frozen plasma (28.6%) and sterile water (20.0%) (P = 0.0

160 Fresh-frozen plasma (FFP) and intermediate purity factor VIII

161 tized mice were transfused with murine fresh-frozen plasma (mFFP), PCC, mixtures of human vitamin K-d

162 fused with all three blood components (fresh frozen plasma [FFP], PLTs, and cryoprecipitate) versus 8

163 5 received blood products (15 received fresh frozen plasma and eight received red blood cell concentr

164 Fresh frozen plasma and immunoglobulins were administered afte

165 Fresh frozen plasma and packed red cells were the most frequen

166 om prothrombin complex concentrates or fresh frozen plasma are, to date, the only option.

167 ed risk of VTE, whereas transfusion of fresh frozen plasma had no effect.

168 hagic shock; however, the exact RBC to fresh frozen plasma ratio is still unclear.

169 Higher fresh frozen plasma ratios (> 1:2) were not associated with in

170 Higher fresh frozen plasma ratios were associated with lower 24-hour

171 PRACTICE ADVICE 6: The large volume of fresh frozen plasma required to reach an arbitrary internation

172 At week 6, 159 frozen plasma samples from Burkina Faso and South Africa

173 Patients who met inclusion criteria had frozen plasma samples tested for HIV-1 viral load.

174 Fresh frozen plasma-to-packed RBCs and platelets-to-packed RBC

175 Ratios of fresh frozen plasma:packed RBC and platelet:packed RBC.

176 (>= 1:1) and medium (>= 1:2 and < 1:1) fresh frozen plasma:packed RBC ratio groups, respectively, com

177 mo analog of the drug chloroquine in rapidly frozen Plasmodium falciparum-infected red blood cells.

178 ubiquitination, and proteasome activities in frozen postmortem OFC tissue from 76 (38 schizophrenia,

179 We applied this method to frozen postmortem samples of human cerebral cortex and r

180 esulted in the correct identification of all frozen poultry samples instead of 93%.

181 tive cut-off limit distinguishing fresh from frozen poultry.

182 Frozen, pre-fried potatoes were fried at 180 degrees C f

183 native mucus from the tissue and storing it frozen prior to use may disrupt its physiological micros

184 237 samples of frozen produce were sampled from different supermarkets

185 nce to control pesticide residues of Chilean frozen produce, since it represents a latent health thre

186 iling on mixtures of cell lines, nuclei from frozen prostate tumors, and biopsy washes.

187 rry, jabuticaba, raspberry, caja and soursop frozen pulps (FPs) using a digestion model coupled with

188 e stool specimens matched norovirus found in frozen raspberries imported from China that were used to

189 uivalent to those of conventionally prepared frozen reference BMD panels for a number of drugs, inclu

190 The validation was performed using custom frozen reference BMD panels prepared in-house at the Cen

191 The event vaporized frozen reservoirs of water and injected large amounts of

192 s, we sequenced RNA transcripts in five snap frozen retinoblastomas which invaded the optic nerve and

193 From the fresh frozen samples (n = 237), a subset had matched microarra

194 0-95% of the maximal respiratory capacity in frozen samples and can be applied to isolated mitochondr

195 ibe a novel respirometry approach suited for frozen samples by restoring electron transfer components

196 a T cells and IgA deposits, where previously frozen samples have been needed, were successful in PAXg

197 h-numerical-aperture objective lens to image frozen samples in their native state.

198 , detected in fresh tissue, are preserved in frozen samples years after collection.

199 educed dissociation bias, compatibility with frozen samples, elimination of dissociation-induced tran

200 try and found to be significantly higher for frozen samples.

201 rofiling with minimal tissue pretreatment in frozen samples.

202 overage than destructive and labor-intensive frozen section analysis techniques.

203 Respondents used Mohs surgery, frozen section or multi-stage excision with delayed clos

204 ccurate identification of prostate cancer in frozen sections at the time of surgery can be challengin

205 y apparent tumor was removed, intraoperative frozen sections were sent.

206 amplify global climate change, because when frozen sediments thaw it unlocks soil organic carbon.

207 d to secondary care for treatment of primary frozen shoulder were recruited from 35 hospital sites in

208 ed for the treatment of adhesive capsulitis (frozen shoulder) and to restore glenohumeral ROM in shou

209 lease are costly and invasive treatments for frozen shoulder, but their effectiveness remains uncerta

210 nts were adults (>=18 years) with unilateral frozen shoulder, characterised by restriction of passive

211 We aimed to establish whether elective frozen single blastocyst transfer improved singleton liv

212 Frozen single blastocyst transfer resulted in a higher s

213 Frozen single blastocyst transfer resulted in higher rat

214 rstimulation syndrome (four of 825 [0.5%] in frozen single blastocyst transfer vs nine of 825 [1.1%]

215 Frozen single blastocyst transfer was associated with a

216 en were randomly assigned to either fresh or frozen single blastocyst transfer.

217 Hitherto, ancient frozen soils have proved excellent in preserving DNA mol

218 spiration is highly temperature sensitive in frozen soils, as liquid water and solute availability de

219 her growth of the focal plant in all but the frozen soils.

220 ng much of the Arctic winter, despite deeply frozen soils.

221 icals generated by UV-light irradiation of a frozen solution containing a fraction of pyruvic acid (P

222 of substrates and proposed intermediates in frozen solution.

223 Frozen-solution EPR data demonstrate the formation of an

224 e structures present and demonstrate that in frozen solutions (at 5 K) 4 forms more extended molecule

225 ucture determination is the preparation of a frozen specimen suitable for high-resolution imaging.

226 ertussis A total of 1,103 fresh and residual frozen specimens from eight clinical sites were tested.

227 Stored frozen specimens from every examination phase were proce

228 ed on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydr

229 For retrospective frozen specimens, the overall PPA and NPA for both targe

230 Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform-methanol solvent, f

231 re, we report a self-peeling phenomenon of a frozen splat that is driven by the existence of a transi

232 ffer (pH 8) was used in the pre-treatment of frozen star fruit (Averrhoa carambola) by immersion or v

233 lication of AFPs can increase the quality of frozen star fruit.

234 ical testing are stored in a refrigerated or frozen state prior to use.

235 Initial studies concentrated on the static, frozen state, whilst more recent studies have accessed t

236 heoretically predicted crossover into a spin-frozen state.

237 A "frozen" state with negligible motion of domain walls on

238 Systems of highly degenerate frozen states may exhibit the rare and counterintuitive

239 Frozen stool samples were collected from 10 healthy volu

240 olite composition in healthy participants in frozen stools.

241 ation in lemon juice solution), freezing and frozen storage (FS) on single and total polyphenols (fre

242 y filleted well-fed Atlantic mackerel during frozen storage at -25 degrees C and effect of treatment

243 protein oxidative stability during long-term frozen storage compared to unaged beef.

244 Frozen storage did not substantially alter IUPM (-18%(-5

245 ity and gel network stability increased upon frozen storage due to protein denaturation.

246 oteins in frozen durian pulps indicated that frozen storage has affected proteins in many ways, espec

247 The molecular changes during frozen storage increased the browning reactions due to p

248 lant extract to limit lipid oxidation during frozen storage of Baltic herring mince and to determine

249 es to inhibit TMAO-demethylase enzyme during frozen storage of fish minces.

250 ant information regarding the opportunity of frozen storage of insect larvae for both research and in

251 The effect of frozen storage on the chemical properties and ingredient

252 onent (1/T(21)) increased significantly upon frozen storage or pH increase, whereas water or NaCl add

253 2-fold change), supporting continued use of frozen storage to allow transport and batched analysis o

254 ferent abundance between fresh and long term frozen storage were identified using two-dimensional pol

255 pulp upon pasteurization, during 12months of frozen storage, and in butia nectar after a 3-month stor

256 r proteins were up-regulated after a year of frozen storage.

257 Major changes occurred in the first week of frozen storage.

258 ne synthase isoform were up-regulated during frozen storage.

259 tability of fresh and thawed lamb leg chops, frozen stored for 3, 6 and 9months.

260 sis and digestibility of fresh and long-term frozen-stored dry-aged lean beef were studied.

261 er amounts of accessible micronutrients than frozen strawberries, while increased bile contents in in

262 sualization and super-resolution imaging for frozen systems.

263 tration of some metabolites in reference and frozen-thawed fish during its storage.

264 ause of myofibrillar protein denaturation in frozen-thawed meat.

265 and/or thawing media enhanced the ability of frozen-thawed oocytes to resume meiosis.

266 esh poultry, visually indistinguishable from frozen-thawed poultry, presents an attractive target for

267 blastocysts were cryopreserved and a delayed frozen-thawed single blastocyst transfer was done.

268 The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cy

269 assay, we measured and correlated the DFI of frozen-thawed sperm from 83 unique mutant mouse strains

270 minated with either fresh, papain-treated or frozen-thawed spermatozoa.

271 fferent (1/5 fresh, 1/4 papain-treated, 0/17 frozen-thawed; P = 0.10).

272 While frozen, the turbulence can influence the pinch-off throu

273 8 h, (3) frozen then formalin-fixed, and (4) frozen then ethanol-fixed and paraffin-embedded (n = 6/g

274 , (2) direct-fixed in formalin for 18 h, (3) frozen then formalin-fixed, and (4) frozen then ethanol-

275 ed cells whose physiological progression is "frozen." This approach, termed in vitro BioID (ivBioID),

276 nd DNA methylation data were generated using frozen tissue from the dorsolateral prefrontal cortex.

277 etabolites from pooled homogenate of FFPE or frozen tissue ranged 86-112%.

278 tional genomic data sets from primates using frozen tissue samples, including many data sets from our

279 jective diagnosis in minutes using a routine frozen tissue section.

280 tup for the analysis of cell cultures, fresh-frozen tissue sections and in vivo experiments on skin.

281 l intraoperative histopathologic analysis of frozen tissue sections can be time-consuming and present

282 in situ probes that can be applied to fresh-frozen tissue sections in a manner analogous to immunofl

283 Importantly, oxidative degradation of fresh-frozen tissues begins within the normal time scale of MA

284 aining single cell transcriptomes from human frozen tissues can provide insight missed by either huma

285 Histological sections of frozen tissues were stained by immunohistochemistry and

286 man lung cancer tissue microarrays and fresh frozen tissues, we found that the overexpression of DNMT

287 ies reversibly binds N(2) in solution: flash frozen toluene solutions of 1 disclose entirely differen

288 Herein, frozen tumors from 36 MGMT methylated patients grouped a

289 imary, 41 metastatic, and 17 recurrent fresh-frozen tumors from 77 patients along with matched normal

290 HR-MAS was performed on pre-treatment frozen tumour tissue from a single centre.

291 Models also estimated the effect of testing frozen versus fresh samples.

292 Archival frozen viable PBMC were obtained from the UCLA Multicent

293 ad staling (crumb moisture loss, decrease in frozen water content, formation of amylopectin crystals,

294 ld that depends directly on the shape of the frozen water layer set by the support foil.

295 otides from monophosphorylated precursors in frozen water-ice.

296 mary neural cell culture to collect EVs from frozen whole murine and human neural tissues by serial c

297 illed parent state is usually electronically frozen with an antiferromagnetic order that resists exte

298 entrations remained stable when samples were frozen within 24 h (mean absolute difference 0.2 pg/ml;

299 unaffected at 4-8 degrees C when samples are frozen within 24 h and single aliquots can be used up to

300 r Australian fish species, in the Australian Frozen Zoo.