ライフサイエンスコーパス: fucosyltransferase

1 surface expression of Notch and a protein O-fucosyltransferase.

2 at is defective for sialylation and alpha1,3-fucosyltransferase.

3 syltransferase 2 is in fact a TSR-specific O-fucosyltransferase.

4 sferase activity with minimal effects on the fucosyltransferase.

5 genes confirm that each encodes an alpha(1,2)fucosyltransferase.

6 compared with those generated with exogenous fucosyltransferase.

7 y the PgtA galactosyltransferase but not the fucosyltransferase.

8 omosome 19q13.3 that encodes three alpha(1,2)fucosyltransferases.

9 ha, a key regulator of expression of various fucosyltransferases.

10 organs, indicating that Arabidopsis thaliana fucosyltransferase 1 (AtFUT1) accounts for all of the xy

11 ana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes the regiospecifi

12 ed in LewisX biosynthesis, naming it chicken fucosyltransferase 1 (CFT1).

13 ed the subcellular localization of protein O-fucosyltransferase 1 (O-FucT-1), which is responsible fo

14 We found that in Drosophila, Protein O-fucosyltransferase 1 (OFUT1), an enzyme that glycosylate

15 ke repeats that may be modified by protein O-fucosyltransferase 1 (Pofut1), an essential component of

16 ith mutations in keratin 5 (KRT5), protein O-fucosyltransferase 1 (POFUT1), or protein O-glucosyltran

17 ied by O-fucosylation catalyzed by protein O-fucosyltransferase 1 (Pofut1).

18 rated that an enzyme distinct from protein O-fucosyltransferase 1 adds O-fucose to TSRs.

19 O-linked glycosylation mediated by protein O-fucosyltransferase 1 and Fringe.

20 we show that mouse embryos lacking protein O-fucosyltransferase 1 die at midgestation with severe def

21 Here we show that the protein O-fucosyltransferase 1 gene (Pofut1), which encodes a glyc

22 othesis and show the following: 1) protein O-fucosyltransferase 1 is indeed the enzyme that adds O-fu

23 the transfer of fucose to Notch by protein O-fucosyltransferase 1 is necessary for Fringe to function

24 A known homologue of O-fucosyltransferase 1 is putative protein O-fucosyltransf

25 Protein O-fucosyltransferase 1 is therefore an essential core memb

26 lacking both maternal and zygotic protein O-fucosyltransferase 1, a cell-autonomous and essential co

27 ss these issues, the gene encoding protein O-fucosyltransferase 1, an enzyme required for Notch ligan

28 O-Fucosylation is mediated by protein O-fucosyltransferase 1, and down-regulation of this enzyme

29 Like O-fucosyltransferase 1, O-fucosyltransferase 2 is conserve

30 However, embryos null for protein O-fucosyltransferase 1, the enzyme that adds O-fucose to E

31 n, a reaction that is catalyzed by protein O-fucosyltransferase-1 (Pofut1).

32 ted by O-fucosylation (mediated by protein O-fucosyltransferase-1) and Fringe, a beta1,3-N-acetylgluc

33 Another alpha1,3-fucosyltransferase gene, fucosyltransferase 10 (Fut10), is expressed in the ventr

34 n of innate lymphoid cells and expression of fucosyltransferase 2 (Fut2) by IL-22-stimulated IECs.

35 Fucosyltransferase 2 (FUT2) controls the production of d

36 d group antigens, which are expressed by the fucosyltransferase 2 (FUT2) gene.

37 Genetic variants of fucosyltransferase 2 (FUT2) have been identified in geno

38 ncentration and the 461G-->A polymorphism of fucosyltransferase 2 (FUT2), a gene associated with susc

39 of intestinal epithelial cells, catalyzed by fucosyltransferase 2 (Fut2), is a major glycosylation me

40 e four TSRs in T. gondii MIC2 with protein O-fucosyltransferase 2 (POFUT2) acceptor sites are modifie

41 Protein O-fucosyltransferase 2 (POFUT2) and B3GLCT work sequential

42 bospondin type 1 repeats (TSRs) by protein O-fucosyltransferase 2 (POFUT2) and is elongated with gluc

43 plasmic reticulum-localized enzyme protein-O-fucosyltransferase 2 (POFUT2) was described for TSRs of

44 When the expression of protein O-fucosyltransferase 2 (POFUT2), the enzyme that transfers

45 f thrombospondin type 1 repeats by protein O-fucosyltransferase 2 (POFUT2).

46 sus sequence for O-fucosylation by protein O-fucosyltransferase 2 (POFUT2).

47 rmore, we expressed recombinant Drosophila O-fucosyltransferase 2 and showed that it O-fucosylates TS

48 Among secretors only [fucosyltransferase 2 gene (FUT2) positive], there were p

49 O-glycans to MIC2 is mediated by a protein O-fucosyltransferase 2 homolog (TgPOFUT2) encoded by the T

50 Like O-fucosyltransferase 1, O-fucosyltransferase 2 is conserved from Caenorhabditis el

51 These results demonstrate that O-fucosyltransferase 2 is in fact a TSR-specific O-fucosyl

52 We also found that O-fucosyltransferase 2 is predominantly localized in the e

53 e show that RNAi-mediated reduction of the O-fucosyltransferase 2 message significantly decreased TSR

54 Although O-fucosyltransferase 2 was assumed to be another protein O

55 The cDNA sequence encoding O-fucosyltransferase 2 was originally identified during a

56 n hepatocyte nuclear factor 4 alpha [HNF4A], fucosyltransferase 2, serpin family A member 1 [SERPINA1

57 O-fucosyltransferase 1 is putative protein O-fucosyltransferase 2.

58 ction mechanism similar to that of protein-O-fucosyltransferase 2.

59 this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose

60 Individuals with a functional fucosyltransferase-2 gene, known as secretors, have incr

61 DM4, IDDM5, IDDM6, IDDM8, and IDDM10 and the fucosyltransferase-2 locus for linkage in sib pairs with

62 of glycosyltransferase expression identified fucosyltransferase 3 (Fut3) as the key enzyme driving sL

63 reatic disease, we inducibly expressed human fucosyltransferase 3 and beta1,3-galactosyltransferase 5

64 trate that hematopoietic progenitors lacking fucosyltransferase 4 and 7 do not express functional PSG

65 common loss-of-function, missense variant in Fucosyltransferase 6 (FUT6; rs17855739,p.Glu274Lys, P =

66 Expression of fucosyltransferase 6 resulted in marked increases in lev

67 overcome this deficiency, we expressed human fucosyltransferase 6 using modified mRNA.

68 nds contribute to metastasis using alpha(1,3)fucosyltransferase 7 (Fuc-TVII(-/-))-deficient mice.

69 e examined the transcriptional regulation of fucosyltransferase 7 (FUT7), an enzyme crucial for gener

70 f N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is in

71 tion of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the

72 The up-regulation of fucosyltransferase 8 (FUT8), the only enzyme catalyzing

73 6-dehydratase (Gmds) and to a lesser extent fucosyltransferase 8 (Fut8).

74 n this study, we examined mice deficient for fucosyltransferase 9 (Fut9), which is thought to synthes

75 -strand mispairing in a poly(C) tract of the fucosyltransferase A (fucT1) gene.

76 nd murine neutrophils expressed low alpha1-3-fucosyltransferase activities.

77 cDNA, significantly raising their levels of fucosyltransferase activity and surface sLe(x/a).

78 and a nonradioactive, fluorescence assay for fucosyltransferase activity have been developed.

79 (AtFUT1) accounts for all of the xyloglucan fucosyltransferase activity in Arabidopsis.

80 ssage significantly decreased TSR-specific O-fucosyltransferase activity in Drosophila S2 cells.

81 thesis of each occurs at different levels of fucosyltransferase activity in intact cells.

82 ed sLe(x/a) is because of decreased alpha3/4-fucosyltransferase activity in MARY-X.

83 yzed by FT85, a 768-amino acid protein whose fucosyltransferase activity maps to the C-terminal half

84 In addition, the alpha1,2-fucosyltransferase activity of GST-WbsJ was found to be

85 vel insights into the specific role of the O-fucosyltransferase activity of SPY in modulating the cir

86 alylated structures by high-level alpha(1,2)-fucosyltransferase activity reduces monocyte adherence a

87 that Fut10 is involved in a unique alpha1,3-fucosyltransferase activity with stringent substrate spe

88 ialyl Lewis x, sialyl Lewis a, alpha(1,3/1,4)fucosyltransferase activity, and FUT3 transcript, but an

89 ilitate folding of Notch did not require its fucosyltransferase activity.

90 exhibits beta-galactosyltransferase but not fucosyltransferase activity.

91 CHO cells also lack alpha1 3 fucosyltransferase activity.

92 genic mice by expressing a human alpha 1,3/4-fucosyltransferase (alpha 1,3/4-FT; EC 2.4.1.65) along t

93 g GDP-fucose:N-acetylglucosaminyl alpha(1,3) fucosyltransferase (alpha(1,3)-Fuc-T) activity was recen

94 in-of-function mutant expresses an alpha(1,3)fucosyltransferase (alpha(1,3)Fuc-T) activity that gener

95 6 melanoma was transfected with the alpha1,2-fucosyltransferase (alpha1,2FT) cDNA.

96 n H-type GDPFuc:beta-D-galactoside alpha1, 2-fucosyltransferase (alpha1,2FT) was stably transfected i

97 antial redundancy in the mammalian alpha-1,3-fucosyltransferase and alpha-1,2-fucosyltransferase gene

98 ts, enzymatic transfers with a milk alpha1,3-fucosyltransferase and an alpha2,3-sialyltransferase (ST

99 eting expression of the endogenous alpha-1,3-fucosyltransferase and beta-1,2-xylosyltransferase genes

100 mportant breakthroughs in the development of fucosyltransferase and fucosidase inhibitors.

101 ibitory activities correlated inversely with fucosyltransferase and sialyltransferase activity based

102 scale) of an endoplasmic reticulum-resident fucosyltransferase and two potential anticancer protein

103 hat the common motif shared by both alpha1,2-fucosyltransferases and alpha1,6-fucosyltransferases hav

104 ences in the relative activities of alpha1,3-fucosyltransferases and alpha2,3-sialyltransferases in t

105 Fucosyltransferases and fucosidases, the main enzymes in

106 involved in glycan modifications, including fucosyltransferases and sialyltransferases, during infla

107 C79a/Ig-alpha, C79b/Ig-beta, and Fut3/alpha-fucosyltransferase); and (ii). variants of bacteriophage

108 yltransferase1 (GALT1), Arabidopsis alpha1,4-fucosyltransferase, and Rattus norvegicus alpha2,6-sialy

109 lysis predicts that these family members are fucosyltransferases, and we first hypothesized that some

110 Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cel

111 These data demonstrate that fucosyltransferases are important in the pathogenesis of

112 e various studies, the specificities of many fucosyltransferases are still unknown, so new approaches

113 human FucT using a structure of a bacterial fucosyltransferase as a template demonstrated that the a

114 nsferase, and Helicobacter mustelae alpha1-2-fucosyltransferase, as efficient tools for live-cell gly

115 yloglucan because of a point mutation in the fucosyltransferase AtFUT1.

116 acid similarity with the xyloglucan-specific fucosyltransferase AtFUT1.

117 requirement for E-selectin ligands, alpha1,3 fucosyltransferases, beta1 and alphaVbeta3 integrins, an

118 ls, however, requires expression of alpha1-3-fucosyltransferases but not PSGL-1.

119 whether high-level expression of alpha(1,2)-fucosyltransferase by porcine endothelium would reduce h

120 ficant differences in expression of alpha1,3-fucosyltransferases, C2GnT (Core2 transferase), or P-sel

121 A novel fucosyltransferase (cFTase) activity has been enriched o

122 gain-of-function experiments where all three fucosyltransferases conferred E-selectin-mediated rollin

123 Fut7 encodes an alpha1,3-fucosyltransferase critical for biosynthesis of glycan l

124 Although overexpression of fucosyltransferase-defective Pofut1 R245A in Pofut1-/- c

125 Furthermore, studies with fucosyltransferase-deficient mice suggest that sialylate

126 glucosaminyltransferases IV and V and alpha6-fucosyltransferase during normal pregnancy.

127 ions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel sp

128 -fucose:beta(1-->4)-D-galactosyl-R 2-alpha-L-fucosyltransferase enzymes (EC 2.4.1.69) responsible for

129 leles of the Pofut1 gene, which encodes an O-fucosyltransferase essential for Notch-ligand binding.

130 egrins and targeted deletion of an alpha(1,3)fucosyltransferase essential for selectin ligand synthes

131 re 1 beta 3-galactosyltransferase and alpha2-fucosyltransferase exhibit unique peptide/glycopeptide s

132 helial cell line transfected with alpha(1,2)-fucosyltransferase, expressing reduced surface expressio

133 y factors resulted in the down-regulation of fucosyltransferase expression, reflected by altered glyc

134 yltransferase (ST) activity and low alpha1,2-fucosyltransferase (FT) activities were detected from du

135 Here we report the discovery of a unique fucosyltransferase (FT) in Caenorhabditis elegans.

136 We demonstrate that alpha1,3 fucosyltransferases (FT) 3, 6, and 7 are markedly elevat

137 redominant HUVEC sialyltransferases (ST) and fucosyltransferases (FT), key enzymes in sLe(x) and Le(x

138 A 60-kilodalton fucosyltransferase (FTase) that adds this residue was pu

139 or murine neutrophils by exogenous alpha1-3-fucosyltransferase FTVI and GDP-fucose created many new

140 To determine how the alpha(1,3)fucosyltransferases Fuc-TIV and Fuc-TVII, and the select

141 en in wild-type mice; mice lacking alpha 1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lackin

142 e sialyltransferase ST3Gal-III compared with fucosyltransferases Fuc-TIV/VII in the synthesis of the

143 have been characterized against recombinant fucosyltransferase (Fuc-T) V using ESI-MS/MRM.

144 s deficient in the expression of alpha-(1, 3)fucosyltransferase (Fuc-TVII), an enzyme known to be req

145 or both PSGL-1 and its modifying alpha-(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infect

146 Induction of the alpha1,3-fucosyltransferase FucT-VII in T lymphocytes is crucial

147 The alpha(1,3)-fucosyltransferase FucT-VII is essential for the biosynt

148 r laboratories indicated that the alpha(1,3)-fucosyltransferase FucT-VII regulates the synthesis of E

149 ed prominently but incompletely by alpha(1,3)fucosyltransferase FucT-VII-dependent fucosylation.

150 E-selectins and that the leukocyte alpha(1,3)fucosyltransferases FucT IV and FucT VII do not provide

151 tin ligand biosynthesis include the alpha1,3-fucosyltransferases FucT-VII and FucT-IV, one or more si

152 In plants, alpha1,3-fucosyltransferase (FucT) catalyzes the transfer of fuco

153 We hypothesized that fucosyltransferase (FucT) enzymes were important pathoph

154 his deficiency results from reduced alpha1,3-fucosyltransferase (FucT) expression and activity in the

155 A GDP-fucose:GM1 alpha1-->2 fucosyltransferase (FucT) is induced during early stages

156 absent in mice doubly deficient in alpha1,3-fucosyltransferase (FucT)-IV and FucT-VII.

157 in ligands must be fucosylated by alpha(1,3)-fucosyltransferase (FucT)-IV or FucT-VII as rolling is a

158 Human alpha1,3/4-fucosyltransferase (FucT)-V catalyzes primarily the synt

159 alpha-(1,3)-Fucosyltransferase (FucT)-VII-deficient OT-I cells displ

160 The activity of leukocyte alpha1,3-fucosyltransferases (FucT-IV or FucT-VII) is an essentia

161 The alpha1,3-fucosyltransferase, FucT-VII, is crucial for the formati

162 Human alpha1,3 fucosyltransferases (FucTs) contain four highly conserve

163 acid sequence alignment of human alpha1, 3/4-fucosyltransferases (FucTs) demonstrates that three high

164 y of domain swap mutants of human alpha1,3/4-fucosyltransferases (FucTs) III and V has been carried o

165 transfer of two of these (the core alpha1,3-fucosyltransferase FUT-1 and the core alpha1,6-fucosyltr

166 cosyltransferase FUT-1 and the core alpha1,6-fucosyltransferase FUT-8) were previously characterized.

167 e surface glycans by inhibition of alpha(1,3)fucosyltransferase (FUT) gene expression is an attractiv

168 a GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT) in an antisense orientation in

169 Histo-blood group antigens (HBGAs) such as fucosyltransferase (FUT)2 and 3 may act as innate host f

170 The xyloglucan-specific alpha2-fucosyltransferase FUT1 catalyzes the transfer of fucose

171 gars in the OB is regulated by the alpha(1-2)fucosyltransferase FUT1.

172 ha1,6-fucosylation levels by up-regulating N-fucosyltransferases FUT1, FUT4 and FUT8 expression, resp

173 intestinal fucosylation via induction of the fucosyltransferase Fut2.

174 phenotype due to knockout of their alpha1-2 fucosyltransferase (Fut2) gene.

175 in binding activity mediated by the alpha1-3 fucosyltransferases Fut3/Fut6 and Glg1 are instrumental

176 contributions of all three myeloid alpha1,3-fucosyltransferases (FUT4, FUT7, and FUT9) to selectin-l

177 The mammalian alpha1,6-fucosyltransferase (FUT8) catalyzes the core fucosylatio

178 r the inability of the biosynthetic alpha1,6-fucosyltransferase (FUT8) to directly fucosylate full-si

179 e fucosylation) is catalyzed by an alpha-1,6-fucosyltransferase (FUT8).

180 In this case, the third alpha1,3-fucosyltransferase FUT9 played an important role because

181 XyG fucosyltransferase (FUTase), encoded by the Arabidopsis

182 hat AtFUT4 and AtFUT6 genes encode alpha(1,2)fucosyltransferases (FUTs) for AGPs.

183 n was homozygous recessive for the alpha(1,2)fucosyltransferase gene (FUT2) in the ABH histo-blood gr

184 infection, and expression of the alpha(1,2) fucosyltransferase gene (FUT2) responsible for the secre

185 from transgenic pigs expressing the alpha1,2 fucosyltransferase gene (H-transferase or HT) gene into

186 located between the secretor type alpha(1,2)-fucosyltransferase gene cluster (FUT1-FUT2-FUT2P) and th

187 lon, a 3.3-kb FUT2 mRNA represents the major fucosyltransferase gene expressed.

188 n alpha-1,3-fucosyltransferase and alpha-1,2-fucosyltransferase gene families.

189 cloned and expressed the chicken alpha(1,3)-fucosyltransferase gene involved in LewisX biosynthesis,

190 othelium by transfection with the alpha(1,2)-fucosyltransferase gene reduced susceptibility to human

191 Another alpha1,3-fucosyltransferase gene, fucosyltransferase 10 (Fut10),

192 es directed at the human Lewis alpha(1,3/1,4)fucosyltransferase gene, FUT3.

193 nce of the repeats, including the alpha(1-2) fucosyltransferase gene, necessary for the synthesis of

194 es from the open reading frames of the mouse fucosyltransferase genes corresponding to human FUT1, FU

195 hese and other results suggest that multiple fucosyltransferase genes in C. elegans may encode enzyme

196 motif which may be of use in identifying new fucosyltransferase genes.

197 ing HAR is the expression of human alpha1, 2-fucosyltransferase (H-transferase, HT).

198 ndothelial cell transfection with alpha(1,2)-fucosyltransferase has been shown to reduce terminal sia

199 th alpha1,2-fucosyltransferases and alpha1,6-fucosyltransferases have similar functions.

200 Expression of human alpha1,2-fucosyltransferase (HT) in the donor cell or tissue prot

201 dentification of the gene encoding protein O-fucosyltransferase I now makes possible mutational strat

202 on of amino acids found in human alpha1, 3/4-fucosyltransferase III (FucT III) conferred a significan

203 ted from CHO cells cotransfected with either fucosyltransferase III (sPSGL-1/Fuc-TIII) or fucosyltran

204 cells were stably transfected with alpha1, 3-fucosyltransferase III to express sialyl Lewis X structu

205 t that the AtFUT family is likely to include fucosyltransferases important for the synthesis of wall

206 lly identified during a data base search for fucosyltransferases in Drosophila.

207 s, we have investigated the role of alpha1,3-fucosyltransferases in generating E-selectin ligands, an

208 quences and confirm the primacy of alpha(1,3)fucosyltransferases in the synthesis of selectin ligands

209 ting E-selectin ligand-synthesizing alpha1,3 fucosyltransferases in transgenic adenoma of mouse prost

210 Despite the broad role of both selectins and fucosyltransferases in various inflammatory, immune and

211 binding or for localization of the alpha1,2-fucosyltransferase involved in O-antigen biosynthesis.

212 loitation of the specificity of the enzymes (fucosyltransferases) involved in fucosylation is a recur

213 otein ligand-1 (PSGL-1) modified by alpha1,3-fucosyltransferase is the principal selectin ligand on s

214 t evidence that FUT8, the mammalian alpha1,6-fucosyltransferase, is the sole enzyme responsible for t

215 cosylated sLe(x) receptors, and two enzymes, fucosyltransferase IV (FucT-IV) and VII (FucT-VII), are

216 The alpha1,3-fucosyltransferase IV (FucTIV) encoded by its gene (FUTI

217 d alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV and VI were determined, and the r

218 d alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV and VI.

219 Mice deficient in alpha1,3 fucosyltransferases IV and VII (FTIV/VII) cannot synthes

220 2 beta1,6-N-acetylglucosaminyltransferase or fucosyltransferases IV/VII were impaired for engraftment

221 Fucosyltransferase-IV and -VII double knockout (FtDKO) m

222 hat express the Fut9 gene encoding alpha(1,3)fucosyltransferase IX (alpha(1,3) Fuc-TIX).

223 We also stably expressed human alpha1,3-fucosyltransferase IX in the L8-GalNAcT cells to establi

224 fferently to conformational changes, and the fucosyltransferases lost less activity than the sialyltr

225 fucosylated glycoconjugates and an alpha1, 2-fucosyltransferase messenger RNA in the small-intestinal

226 veal that FUT-6, another C. elegans alpha1,3-fucosyltransferase, modifies nematode glycan cores, spec

227 ferase 2 was assumed to be another protein O-fucosyltransferase, no biochemical characterization exis

228 In contrast to alpha1,6-fucosyltransferases, none of the mutants of WbsJ within

229 th factor-like repeats, GDP-fucose protein O-fucosyltransferase (O-FucT-1), was purified previously f

230 nalysis reveals that, unlike all other known fucosyltransferases, O-FucT-1 is a soluble protein that

231 er of the large GT-B fold family, like other fucosyltransferases of known structures, it contains a v

232 verify that TgSPY is the nucleocytoplasmic O-fucosyltransferase (OFT) by 1) complementation with TgSP

233 and its ligands are modified by a protein O-fucosyltransferase (OFUT1) that attaches fucose to a Ser

234 Together with the fucosyltransferase POFUT2, B3GLCT adds Glucosebeta1-3Fuc

235 Using an alpha-1,3-fucosyltransferase preparation and enzymatic conditions

236 cosylated FVII EGF-1 was achieved by using O-fucosyltransferase purified from Chinese hamster ovary c

237 state of GDP-fucose complexed with Mn(II) in fucosyltransferase reactions.

238 ly and indicates that sialyltransferases and fucosyltransferases recognize N-acetyllactosamine in a d

239 ndothelial cell transfection with alpha(1,2)-fucosyltransferase reduced terminal sialic acid expressi

240 l mediator of Notch receptors, and Pofut1, a fucosyltransferase required for the activity of Notch re

241 scherichia coli O128:B12 encodes an alpha1,2-fucosyltransferase responsible for adding a fucose onto

242 obiose unit of these N-glycans, but only the fucosyltransferases responsible for transfer of two of t

243 on, sulfation, and fucosylation by alpha 1,3-fucosyltransferase(s) (FucT), are required for functiona

244 The identity of the alpha(1,3)fucosyltransferase(s) expressed in cells that bear L-sel

245 tivity of the sialyltransferases whereas the fucosyltransferases showed some activity, albeit very lo

246 Up to 20 different S. mansoni fucosyltransferase (SmFucT) genes can be found in genome

247 were stably transfected with alpha(1,3/1,4) fucosyltransferase-specific cDNA (B16F10ft), allowing th

248 The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transf

249 studies and structure comparison with other fucosyltransferases suggest that FUT1 uses a SN2-like re

250 function beta3-galactosyltransferase/alpha2-fucosyltransferase that contributes the 2nd and 3rd suga

251 FUT2 encodes the alpha(1,2) fucosyltransferase that determines blood group secretor

252 Pea microsomes contain an alpha-fucosyltransferase that incorporates fucose from GDP-fuc

253 dy binds fucosyltransferase1, an alpha-(1,2)-fucosyltransferase that synthesizes H-type structures on

254 glycoprotein ligand-1 (PSGL-1) and alpha1-3-fucosyltransferases that construct the glycan determinan

255 xpression of Fut genes that encode alpha-1,3-fucosyltransferases, the enzymes that generate the Le(x)

256 ochemically identified to encode an alpha1,2-fucosyltransferase through radioactivity assays, as well

257 trate binding, common strategies employed by fucosyltransferases to coordinate GDP, features that def

258 structure was also compared with other known fucosyltransferases to identify conserved and divergent

259 d cells of GDP-fucose, the substrate used by fucosyltransferases to incorporate fucose into protein a

260 -ligand interactions following the addition (fucosyltransferase-treatment) or removal (deglycosylatio

261 The alpha(1,3)-fucosyltransferases, types IV and VII (FUT4 and FUT7, re

262 alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of

263 cceptor substrate specificity into alpha1, 3-fucosyltransferase V (FucT V), which, under the same ass

264 as the donor substrate for recombinant human fucosyltransferase V, and GDP-d-[3H]arabinosep serves as

265 S) has been developed and demonstrated using fucosyltransferase V.

266 hosphate (GDP) fucose and exogenous alpha1-3 fucosyltransferase VI increased cell-surface sLe(x) dete

267 eated ex vivo for 30 minutes with the enzyme fucosyltransferase-VI and guanosine diphosphate fucose t

268 The selectin pathway recruiter, alpha-1,3-fucosyltransferase-VI enzyme, significantly increased Tr

269 Fucosyltransferase VII (Fuc-T VII) and core 2 beta-1,6-N

270 We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyl

271 trophils also expressed transcripts encoding fucosyltransferase VII (FucT-VII) and Core2GlcNAcT-I, wh

272 pressed equivalently high levels of alpha1,3-fucosyltransferase VII (FucT-VII) as wild-type Th1 cells

273 hese ligands is the expression of alpha(1,3)-fucosyltransferase VII (FucT-VII), a FucT essential for

274 have determined the role of tissue-specific fucosyltransferase VII (FucT-VII), an enzyme necessary f

275 (Stat4)-independent increase in Th1 cells of fucosyltransferase VII (FucT-VII).

276 ed because of targeted mutation of alpha-1,3-fucosyltransferase VII (Fut7).

277 fucosyltransferase III (sPSGL-1/Fuc-TIII) or fucosyltransferase VII (sPSGL-1/Fuc-TVII).

278 tment (resulting from targeted disruption of fucosyltransferase VII [FTVII]), and the absence of matu

279 In this study, fucosyltransferase VII cDNA (Fuc-TVII) transfection of t

280 Th1 cells also expressed more fucosyltransferase VII mRNA than naive or Th2 cells.

281 we transduced Jurkat (JK) T cells expressing fucosyltransferase VII with a chimeric chemokine recepto

282 ese hamster ovary cells, along with CD34 and fucosyltransferase VII, results in ligand activity, as d

283 e cell-surface receptor, PSGL-1, mediated by fucosyltransferase VII, serves as a mechanism for regula

284 arting JK lines, the resulting cell line (JK fucosyltransferase VII-CCR6) migrated 6-fold better to C

285 cotransfected with cDNAs encoding alpha (1,3)fucosyltransferase-VII (FucT-VII) and PSGL-1 rolled on L

286 ontrast to cells cotransfected with alpha1-3 fucosyltransferase-VII (FucT-VII) plus PSGL-1, K562 cell

287 (+) cells express both E-selectin ligand and fucosyltransferase-VII (FucT-VII).

288 -selectin, correlated with elevated alpha1,3-fucosyltransferase-VII messenger RNA levels, but selecti

289 ferases alpha2,3-sialyltransferase, alpha1,3-fucosyltransferase-VII, and core 2 beta1,6 N-acetylgluco

290 ific glycosyltransferases including alpha1,3-fucosyltransferase-VII, core 2 beta1-6-N-glucosaminyltra

291 ated, and negligibly inhibitory, whereas the fucosyltransferase was active toward small substrates.

292 A GDP-fucose:polypeptide fucosyltransferase was purified 5000-fold to homogeneity

293 atode N-glycan core in vitro using all three fucosyltransferases was performed, and the nature of the

294 with another recently characterized alpha1,2-fucosyltransferase (WbsJ) of E. coli O128:B12 indicates

295 In contrast to other alpha1,2-fucosyltransferases, WbwK does not display activity towa

296 se enzymes with Helicobacter pylori alpha1-3-fucosyltransferase, we develop a host-cell-based assay t

297 es of FKP and a Helicobacter pylori alpha1,3 fucosyltransferase, we prepared a library of Le(x) trisa

298 sferase activity and a decrease in alpha(1,3)fucosyltransferases when these cells differentiate towar

299 in CHO LEC11 cells with an active alpha(1,3)-fucosyltransferase, which makes possible the biosynthesi

300 defenses were observed in mutants of several fucosyltransferases with specific substrates (e.g. O-gly