ライフサイエンスコーパス: functional expression

1 her 4 days showed a gradual recovery of VGSC functional expression.

2 own transcripts was validated by cloning and functional expression.

3 itions in the natural sequence that restrict functional expression.

4 of four sodium channel subunits) with robust functional expression.

5 ed, resulting in lower levels of protein and functional expression.

6 g transport to the cell surface and thus its functional expression.

7 R both in vitro and in vivo to ensure proper functional expression.

8 l aggregates (hMSC spheroids) restores CXCR4 functional expression.

9 Kv1.2 cell-surface expression efficiency and functional expression.

10 post-translational modifications to achieve functional expression.

11 s, whereas murine or rat ENaC increases CFTR functional expression.

12 whereas the presence of ENaC increases CFTR functional expression.

13 rexpression did not significantly alter ENaC functional expression.

14 membrane region with hERG and decreases hERG functional expression.

15 s were activated by light pulses, indicating functional expression.

16 hat necessitates mammalian systems to obtain functional expression.

17 the fenamate niflumic acid, confirming their functional expression.

18 fy this enzyme by transcriptome analysis and functional expression.

19 ased total Hsp70 expression ~2-fold and ENaC functional expression ~1.4-fold.

20 activates CAR at the BBB and increases P-gp functional expression, a clinically significant drug-dru

21 d found that KDEL-R depletion decreases ENaC functional expression, again without altering beta-ENaC

22 ating mitochondrial genes to the nucleus for functional expression (allotopic expression) is a challe

23 AHA2 protein biochemistry and functional expression analysis in Xenopus oocytes indica

24 in both plant hosts, and metatranscriptomic (functional expression) analysis revealed that most genes

25 neuronal CaV1.2 channels in vivo: increased functional expression, anchoring of AKAP15 and PKA, and

26 g adolescence may competitively decrease the functional expression and activity of NMDA receptors in

27 bunits has a gain-of-function effect (higher functional expression and agonist sensitivity and sponta

28 Functional expression and analysis of AaCAT1 in Xenopus

29 ta2alpha4)2 nAChRs yielded similar levels of functional expression and Ca(2+) permeability, measured

30 Functional expression and complementation of temperature

31 Herein we describe the cloning, functional expression and initial characterization of OR

32 ur results provide a potential route for the functional expression and manipulation of mammalian tran

33 Our results suggest that functional expression and pharmacological studies may pr

34 As age progresses, alpha subunit functional expression and protein levels diminish in cor

35 The functional expression and redistribution of endothelial

36 tatic regulation of CaV2.1 protein level and functional expression and that RNF138 serves as the prim

37 The high level of functional expression and the low level of endogenous co

38 microelectrode voltage-clamp, we report the functional expression and the molecular, biophysical, an

39 h the increasing availability of large scale functional, expression and evolutionary data.

40 body of previous work using mutagenesis and functional expression, and is now being supplemented by

41 hannel gating, and several T1 sites regulate functional expression as well.

42 te umami taste receptor hTAS1R1-hTAS1R3 in a functional expression assay.

43 manipulate plant processes, high-throughput functional expression assays in plants were performed on

44 tags (ESTs) with virus-based high-throughput functional expression assays in plants.

45 candidate genes enhanced the DeltaF508-CFTR functional expression at the apical PM in human CF bronc

46 ese findings demonstrate that augmenting RFC functional expression at the BBB could effectively compe

47 ) has been identified as a regulator of P-gp functional expression at the BBB.

48 fied in our screen likely impact Kir-channel functional expression at the level of vesicle budding fr

49 udy is to investigate the regulation of BCRP functional expression by peroxisome proliferator-activat

50 les and also class I MHC molecules for their functional expression, characterization and engineering.

51 Through a functional expression cloning approach, a gag-retinoid X

52 Functional expression cloning is a powerful strategy for

53 Using functional expression cloning, we have identified GAS5 (

54 bait, we show the feasibility of a modified functional expression-cloning strategy to identify human

55 We studied their trafficking and functional expression, combining immunocytochemical and

56 Changes in ENaC functional expression correlated with ENaC surface expre

57 This increase in ENaC functional expression corresponded to an increase in ENa

58 so on molecular genetic analysis and ideally functional expression data.

59 Cystic fibrosis (CF) is caused by the functional expression defect of the CF transmembrane con

60 ine 508 (DeltaF508 or Phe508del), results in functional expression defect of the CF transmembrane con

61 by targeted disruption of a PKS gene, and by functional expression in a heterologous Streptomyces hos

62 reased processing, and impaired cell surface functional expression in Calu-3 human airway epithelial

63 ons into the orthologous mouse ZIP4 gene for functional expression in cultured cells.

64 tically enhances DeltaF508 CFTR cell surface functional expression in cystic fibrosis airway epitheli

65 length dehydrogenase clones were screened by functional expression in Escherichia coli using a recent

66 rescent protein (YFP) and first assessed for functional expression in HEK293 cells.

67 en homomeric subunits as expected from their functional expression in heterologous expression systems

68 Functional expression in heterologous system and gene kn

69 ther ion channels have been characterized by functional expression in heterologous systems, and most

70 Functional expression in human embryonic kidney 293 cell

71 ound that Cdk5 may regulate Ca(V)3.2 channel functional expression in rats with mechanical allodynia

72 Screening of these candidate genes by functional expression in Saccharomyces cerevisiae yielde

73 ingosine phosphate lyase gene along with its functional expression in Saccharomyces cerevisiae.

74 We show by functional expression in shoot cells that the different

75 1 voltage-gated K(+) channels) have enhanced functional expression in smooth muscle cells of a primar

76 activity-dependent (auto)regulation of VGSC functional expression in the strongly metastatic Mat-LyL

77 functions because of an inability to obtain functional expression in vitro.

78 tal C-terminal domain is required for normal functional expression in vivo.

79 Functional expression in Xenopus laevis oocytes followed

80 Functional expression in Xenopus oocytes of concatenated

81 NKCC2B variant into the A or F variant, with functional expression in Xenopus oocytes.

82 Functional expression in yeast and Xenopus oocytes revea

83 is genome by direct enzymological assays and functional expression in yeast.

84 tic electrical impulses due to abnormal VGSC functional expression influenced by the latent virus.

85 to plasma membrane for surface insertion and functional expression is a key process regulating signal

86 (SLC26A9) is a promising candidate, but its functional expression is drastically reduced in cells th

87 ssociated with decreased Kir4.1 K(+) channel functional expression, leading to elevated in vivo stria

88 Our study has defined the "MECP2 functional expression module" and identified enhancer an

89 olin-1 coexpression reduced Slo1 surface and functional expression near 70% without affecting channel

90 We examined the in vivo functional expression of 12 selected receptors by microi

91 The functional expression of 95 genes was evaluated in mice

92 Previous studies have suggested an increased functional expression of a cell-surface import system fo

93 e best of our knowledge the first example of functional expression of a cyanobacterial P450 in E. col

94 We thus report the cloning and functional expression of a gene involved in an insect-sp

95 ystem to identify proteins necessary for the functional expression of a mammalian Kir channel at the

96 omolog of UapA, namely rSNBT1, permitted the functional expression of a mammalian NAT in Aspergillus

97 This work represents the full sequencing and functional expression of a marine natural-product pathwa

98 Here we describe the molecular cloning and functional expression of a novel member of the serpin fa

99 Functional expression of a selection Nostoc spp.

100 igonucleotides inhibited NMD and rescued the functional expression of a third LQT2 mutation, Y1078.

101 these structural alterations did not impair functional expression of AE1, the cytosolic and membrane

102 virus have been evaluated for high-level and functional expression of AhR.

103 Functional expression of alpha-amino-3-hydroxy-5-methyl-

104 To test the hypothesis that increased functional expression of alpha4* nAChRs in these neurons

105 SIC2 expression, accounting for the observed functional expression of amiloride-sensitive currents in

106 insertion of adrenergic receptors as well as functional expression of AMPA and NMDA receptors at syna

107 ay enzyme, protein engineering to enable the functional expression of an acyltransferase via traffick

108 novel, unidentified estrogen receptor or to functional expression of an estrogen receptor-alpha spli

109 d intracellularly and that it is the lack of functional expression of ASIC2 at the cell surface that

110 the hypothesis that the AE3 knockout reduces functional expression of astrocytic NBCe1.

111 These observations indicate a functional expression of AtGLR3.5 in this organelle.

112 Differences were found in the functional expression of ATP receptors, TRPA1 channels,

113 n that of TASK-1 and TASK-3, consistent with functional expression of both channel types.

114 anscripts are regulated in a way that alters functional expression of both channels at the membrane.

115 RgIA inhibition of Ca(v)2.2 channels needed functional expression of both human GABA(B) receptor sub

116 onset of hearing.SIGNIFICANCE STATEMENT The functional expression of both VGCCs and BK channels, as

117 oocytes, ERp29 overexpression increased the functional expression of both wild-type and DeltaF508-CF

118 omain disrupted cell surface trafficking and functional expression of Ca(V)2.3.

119 ical model of neuropathic pain increases the functional expression of Ca(V)3.2 channels.

120 h that resulted in the molecular cloning and functional expression of CCH1 by exploiting homologous r

121 Thus, the functional expression of CCR6 is sufficient to provide t

122 Here, we report the identification and functional expression of CD38 from Xenopus laevis, a key

123 together, these observations demonstrate the functional expression of CD40 in epithelial tumors of th

124 ce that disease-associated or injury-induced functional expression of chemokines/receptors in both ne

125 rones promote both the protein level and the functional expression of CLC-1 wild-type and A531V mutan

126 Functional expression of Clock, a circadian clock gene t

127 e sulphonylurea receptor SUR2A can alter the functional expression of cloned ATP-sensitive K(+) chann

128 However, many recent reports describe functional expression of cortactin in different hematopo

129 r biocytin were integrated in a study of the functional expression of corticotropin-releasing factor

130 Functional expression of COX-2 was confirmed by Northern

131 Functional expression of Coxiella bcp was demonstrated b

132 contribute to tumor growth by modulating the functional expression of critical genes involved in tumo

133 Ad-SM22-4A1 drives a smooth muscle-specific functional expression of CYP4A1 and demonstrates increas

134 We show here that dexamethasone rescues functional expression of DeltaF508-CFTR.

135 n removal from target proteins to rescue the functional expression of disparate mutant ion channels t

136 lection fluorescence microscopy to study the functional expression of DOR in sensory neurons from rat

137 in is shown to exhibit 50% dependence on the functional expression of dynamin 2 for its active cellul

138 signaling by the EP(4) receptors induces the functional expression of early growth response factor-1

139 Functional expression of endothelial ICAM-1 and VCAM-1 w

140 In support of this hypothesis, functional expression of Erg channels in a heterologous

141 neuronal membrane excitability by regulating functional expression of Erg channels.

142 have opened the way for the high-throughput functional expression of eukaryotic membrane proteins.

143 Hence, EXP1 is specifically required for the functional expression of EXP2 as the nutrient-permeable

144 lows for the stable uptake, integration, and functional expression of extracellular DNA.

145 Direct proof for functional expression of FcgammaR by Ag-specific CD8 T c

146 A recent study demonstrated functional expression of FFA3 in the rodent sympathetic

147 tion signal and was essential for detectable functional expression of FLP15-R in CHO cells.

148 The functional expression of FLP15-R in mammalian cells was

149 systems is particularly detrimental for the functional expression of G protein-coupled receptors (GP

150 Treatment with genistein both enhanced the functional expression of G551D-CFTR and improved regulat

151 e in the pathology of SE are deficits in the functional expression of GABA(A) receptors (GABA(A)Rs),

152 Chronic ethanol exposure increased the functional expression of GABA(A) receptors in acutely is

153 and that Orai1 and STIM1 expression leads to functional expression of Gd-resistant ROCE.

154 e D1 receptor signaling are required for the functional expression of GluN2B transmission and to sust

155 We show that functional expression of GLUT5 in yeast requires mutatio

156 We found that functional expression of glutamate transporter GLT1 is 4

157 phenotypic expression of CD11a and increased functional expression of granzyme B and interferon-gamma

158 The results indicate that functional expression of HCN3 channels in IGL neurons is

159 reticulum (ER), thus accounting for the poor functional expression of homomeric 1b currents.

160 Mutating the RXR facilitated maturation and functional expression of homomeric hERG 1b channels in a

161 duces cell-surface expression efficiency and functional expression of homomeric Kv1.2 channels.

162 Despite recent progress in the functional expression of hT2Rs in vitro, up until now, h

163 microtubule-based trafficking processes for functional expression of hTHTR1.

164 Such feedback mechanism between the functional expression of I(A) and intracellular Ca(2+) m

165 vidence to demonstrate that the kinetics and functional expression of I(A) are tightly regulated by i

166 athematical modeling, we determined that the functional expression of IKD is reduced in sensory neuro

167 ration, indicating that VEGF/VPF induced the functional expression of IL-8 protein in HBMECs.

168 We have demonstrated that the morphology and functional expression of ionic currents in trans-differe

169 This study aimed to understand the functional expression of K(ATP) channel subunits in dist

170 Here we show that the functional expression of K(Ca) channels in LMNs developi

171 erum into the embryonic hindlimb reduced the functional expression of K(Ca) channels in vivo to level

172 1 at the plasma membrane and is required for functional expression of K(V)10.1 channels.

173 As we could find no evidence for the functional expression of K(V)7.2 in the axons, using the

174 ity act via a common pathway that influences functional expression of K+ channels and proliferative c

175 r mechanism that neurons use to modulate the functional expression of KCC2 remains rudimentary.

176 R (mean~800 Omega cm(2)); it also shows good functional expression of key tight junction proteins, tr

177 itronectin and were consistent with enhanced functional expression of Kv4-family subunits.

178 mechanisms at the cell surface regulate the functional expression of Kv4.2 channels.

179 The functional expression of large-conductance (BK-type) Ca2

180 The functional expression of large-conductance Ca2+-activate

181 rrowed the search for domains that influence functional expression of malpha6*-nAChRs.

182 ts are some of the molecular impediments for functional expression of malpha6mbeta2beta3- or malpha6m

183 s is the first demonstration of heterologous functional expression of mammalian OPN4 in the genetical

184 Functional expression of markers requires Cre-mediated i

185 and respond to bacteria by demonstrating the functional expression of members of the Toll-like family

186 rone machinery regulates the homeostasis and functional expression of misfolded proteins in the cell.

187 direct association with p11 is required for functional expression of Na(V)1.8, disrupting this inter

188 sis of caged carbachol demonstrated that the functional expression of nAChRs is located both on the s

189 e that association with CaM is important for functional expression of NaV1.4 and NaV1.6 VGSCs.

190 However, low functional expression of Nav1.9 in heterologous systems

191 clude that there is spatial variation in the functional expression of NBTI/dipyridamole-sensitive tra

192 Here we show that the functional expression of neuronal N-type Ca(V) channels

193 As we learn more about the functional expression of nicotinic receptors in specific

194 transfections, was essential for detectable functional expression of NPR-1.

195 N-terminal amino acids was not required for functional expression of OEP80 and accumulation of the 7

196 s result may be at least in part due to poor functional expression of olfactory receptors and/or limi

197 This gene (ODR-4) allows for functional expression of olfactory receptors in heterolo

198 Thus, an increase in functional expression of one channel reciprocally modula

199 tion bias of long-range connections with the functional expression of orientation selective neural ac

200 though hypersensitivity to NSC73306 required functional expression of P-gp, biochemical assays reveal

201 ere was no electrophysiological evidence for functional expression of P2X4 receptors on AgRP-NPY neur

202 ken together, the present study demonstrated functional expression of P2X7 receptors in non-neuronal

203 region of Pax3 that are sufficient to direct functional expression of Pax3 in neural crest.

204 dy, we report that Galpha12QL stimulates the functional expression of PDGFRalpha and demonstrate that

205 We examined cellular protein processing and functional expression of photoreceptor cyclic nucleotide

206 By examining the functional expression of plasma membrane transporters at

207 are generally obligatory events required for functional expression of prenylated proteins.

208 Both phenytoin and lamotrigine increased functional expression of R1648C, but lamotrigine also in

209 e wild-type 5-HT(3)A subunit, did not affect functional expression of receptors, suggesting that the

210 to pathological hypertrophy, therefore, the functional expression of repolarizing K(+) (and depolari

211 TP1, RTP1S, supports robust cell-surface and functional expression of representative odorant receptor

212 group has previously reported in vitro that functional expression of RFC at the blood-brain barrier

213 We obtained functional expression of Sav1866 in the drug-sensitive,

214 tomy suggests that GRP94 is required for the functional expression of secretory and/or membrane prote

215 suggesting an essential role for Gp93 in the functional expression of secretory/integral membrane pro

216 SERT substrates also shifted the relative functional expression of SERT by redistributing intracel

217 Here, we report functional expression of SHANK3 in mouse dorsal root gan

218 mice demonstrates that ROMK is essential for functional expression of SK channels in both TAL and CCD

219 sodA sodB-deficient mutant, demonstrated the functional expression of SOD activity by cloned M. catar

220 In this study, we demonstrate that functional expression of Stil is also required for neura

221 3 ligase activity significantly enhanced the functional expression of the A531V mutant.

222 Using S. cerevisiae as host for the functional expression of the C. albicans Fe-uptake prote

223 he biliary output of bile salts (BS) and the functional expression of the canalicular BS export pump

224 K(ATP) channels via SUR1, thereby affecting functional expression of the channel in beta-cell membra

225 ences in the voltage-dependent phenotype and functional expression of the channel.

226 e calcium channels, which leads to increased functional expression of the channel.

227 ouabain-resistant colonies, consistent with functional expression of the chimeric protein and indica

228 Here, we report that functional expression of the Eag channel is temperature-

229 The functional expression of the G protein-coupled P2Y(2) nu

230 esponse to neuronal injury by regulating the functional expression of the glutamate receptor subunits

231 tudy, we directly compared mRNA, protein and functional expression of the high-conductance Ca(2+)-act

232 tional assay also confirmed the cleavage and functional expression of the HIV-1 reverse transcriptase

233 The experiments were comprised of functional expression of the hKAT-I in an insect cell/ba

234 , identified seven deletion strains in which functional expression of the Kir channel at the plasma m

235 adrenergic receptor (beta(2)-AR) facilitates functional expression of the OR M71 at the plasma membra

236 hloride homeostasis due to a decrease in the functional expression of the potassium-chloride cotransp

237 r a late synthetic promoter allowed a higher functional expression of the protein and much stronger (

238 ve been severely hampered by the inefficient functional expression of the receptors in heterologous e

239 was ENaC-specific, as PON-2 had no effect on functional expression of the renal outer medullary potas

240 es in cortical brain slices to show that the functional expression of the slow calcium-activated afte

241 ith activation of Akt and STAT5 and required functional expression of the small GTPases Rho, Rac, and

242 Functional expression of the StV-specific MERG1a channel

243 Functional expression of the telomerase catalytic subuni

244 Significantly, we found that reduced functional expression of the voltage-dependent potassium

245 t ion channels are defined experimentally by functional expression of their pore-forming alpha subuni

246 poor, in part because of limited success in functional expression of these channels.

247 rappreciated, in part, due to relatively low functional expression of these receptors in naive states

248 se construct of the Cl3'NT/NU to dissect the functional expression of these two surface membrane enzy

249 y play a central role in modulating both the functional expression of this critical transporter in th

250 Functional expression of this receptor in human breast c

251 sensitive NMDA receptors, and found that the functional expression of this receptor subtype is strain

252 Interestingly, the functional expression of this transporter at the presyna

253 amined the effect of membrane cholesterol on functional expression of tissue factor (TF), a cellular

254 Functional expression of TRP channels was determined wit

255 ted by distinct blockers consistent with the functional expression of TRPM7 channels.

256 Our data demonstrate functional expression of TRPM7-like channels in mouse oo

257 lation of corneal nerves are due to enhanced functional expression of TRPM8 channels in these injured

258 The functional expression of TRPV1 on airway epithelium has

259 By the functional expression of tsNa(V)1.4, we show how differe

260 tosynthetic rate increases in leaves and the functional expression of tvinv is crucial for such effec

261 Here, we demonstrate functional expression of two mechanically activated chan

262 ector VRT-325, could rescue cell surface and functional expression of two representative Na(V)1.1 mut

263 er transition from C to U and, therefore, on functional expression of uracil N-glycosylase 2, which i

264 cell capture assay to assess changes in the functional expression of vascular endothelial growth fac

265 nd acute infection significantly reduced the functional expression of VGSCs within 24 h and completel

266 ency, viral reactivation, and changes in the functional expression of VGSCs.

267 To examine the relationship of functional expression of VLA-4 to prognosis in AML, we s

268 This study asked whether the functional expression of voltage-gated Ca(2+) channels (

269 eshold of cold thermosensors correlated with functional expression of voltage-gated K channels Kv1.1/

270 Functional expression of voltage-gated Na(+) channels (V

271 physically associate with (and regulate the functional expression of) mouse CD1d on the surface of c

272 Functional expressions of ObAS1 and ObAS2 in Saccharomyc

273 that the ephrin-B2 ligand is likely to have functional expression on angiogenic arterial ECs and ind

274 ions, atherosclerotic plaque deposition, and functional expression on worsening outcomes of women.

275 les introduce the possibility that selective functional expression patterns may provide correlates fo

276 The current work meticulously studies the functional expression profile changes of VGSCs during th

277 ionship between gene-island distribution and functional expression profiling suggests for the first t

278 t the employment of Escherichia coli for the functional expression, purification, and reconstitution

279 In stomata, loss of OsK5.2 functional expression resulted in lack of time-dependent

280 ine phosphorylation sites by mutagenesis and functional expression revealed a multisite regulatory me

281 Functional expression reveals that G406R produces mainta

282 ed insulin resistance and type 2 diabetes, a functional expression screen was performed to search for

283 H) for CaSR mutations and performed in vitro functional expression studies and three-dimensional mode

284 In independent functional expression studies in TNBC models, EPHA2 gene

285 In functional expression studies, mutations lead to a decre

286 A functional expression survey of nearly 200 candidate gen

287 H-bonding residues, based on an established functional expression system for PsN(2)OR in E. coli.

288 Here we used two different functional expression systems to characterize three Cx43

289 receptor sorting to the plasma membrane for functional expression under normal and pathological cond

290 olves a parallel decrease of alpha and beta1 functional expression via a transcript downregulatory me

291 Intracellular labeling was decreased while functional expression was confirmed by whole cell patch

292 ENaC functional expression was decreased by DeltaKEEL ERp29 o

293 The increase in functional expression was not due to a change in the rat

294 Functional expression was successful, with uptake of Ca(

295 The differences in functional expression were associated with changes in bo

296 CRACM mRNA, protein, and functional expression were examined in purified HLMCs an

297 -negative effect of EA2 mutants on CaV2.1 WT functional expression, which can be attributed to defect

298 The increase in mENaC functional expression with coinjection of 10 ng of Hsp70

299 ly developed FACS-based selection system for functional expression with ultradeep 454 sequencing, we

300 trated to interact with ENaC, decreases ENaC functional expression without altering beta-ENaC express