ライフサイエンスコーパス: galactopyranoside

1 of X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside).

2 between protonation and binding of the cargo galactopyranoside.

3 l alpha-D-glucopyranoside and methyl alpha-D-galactopyranoside.

4 feature of a 4,6 cyclic phosphate on a beta-galactopyranoside.

5 ant Glu126-->Asp binds p-nitrophenyl-alpha,D-galactopyranoside.

6 e and enzyme assay with o-nitrophenyl beta-D-galactopyranoside.

7 rophenyl fucopyranoside versus p-nitrophenyl galactopyranoside.

8 ve towards the substrate p-nitrophenyl alpha-galactopyranoside.

9 luorescent substrate C12-fluorescein di-beta-galactopyranoside.

10 t but the mutants retain the ability to bind galactopyranoside.

11 than the WT enzyme on p-nitrophenyl alpha-d-galactopyranoside.

12 - 0.1 that is insensitive to the presence of galactopyranoside.

13 opyranoside and 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside.

14 nten constant (KM) of 1.2 mM for the indolyl galactopyranoside.

15 in and a polar moiety, including the alpha-d-galactopyranoside.

16 at O-3 were prepared from p-methoxyphenyl d-galactopyranoside.

17 binopyranoside but also p-nitrophenyl beta-D-galactopyranoside.

18 to discriminate primarily between gluco- and galactopyranosides.

19 ing a gauche,trans conformation in gluco and galactopyranosides.

20 enzyme-catalyzed hydrolysis of p-nitrophenyl-galactopyranosides.

21 harvested 3.5 h post-isopropyl-1-thio-beta-D-galactopyranoside (0.4 mM)-induction growth at 25 degree

22 2-propyl 2,6-di-O-acetyl-3,4-epithio-alpha-D-galactopyranoside (14) gave the derivative of Glcp-beta-

23 26-O-alpha-l-rhamnopyranosyl (1-->2)-beta-d-galactopyranoside (3a,3b), furosta-2alpha,3beta,22xi,26-

24 e competitive bioassay, 4-aminophenyl beta-d-galactopyranoside (4-APG) conjugated to QDs competes wit

25 ha-D-galactopyranosyl-(6'' --> 1''')-alpha-d-galactopyranoside (5) and benzoyl-O-alpha-D-glucopyranos

26 16-O-alpha-l-rhamnopyranosyl (1-->2)-beta-d-galactopyranoside (5).

27 ha-L-rhamnopyranosyl (1"' --> 6 '')-O-beta-D-galactopyranoside (6).

28 1), and alpha-O-benzyl 2-acetamido-2-deoxy-D-galactopyranoside (8), confirming the critical requireme

29 tes, a colorimetric (chlorophenol red-beta-D-galactopyranoside), a chemiluminescent (Lumi-Gal 530), a

30 of beta,D-galactosylpyranosyl 1-thio-beta,D-galactopyranoside, a property observed with Glu269-->Asp

31 on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) agar plates containing uracil and uri

32 Use of a difluoro-alpha-galactopyranoside allowed trapping of a covalent interme

33 -262-->Trp) with bound p-nitrophenyl-alpha-d-galactopyranoside (alpha-NPG), a high-affinity lactose a

34 o-2,4,6-trideoxy-2-trifluoroacetamido-beta-d-galactopyranoside, an advanced 2-acetamido-4-amino-2,4,6

35 asts with benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, an inhibitor of O-linked glycosylatio

36 se for cleavage of both 4-nitrophenyl-beta-d-galactopyranoside and 4-nitrophenyl-2-deoxy-beta-d-galac

37 rophenyl fucopyranoside versus o-nitrophenyl galactopyranoside and a 300-fold increased substrate spe

38 f the fluorogenic substrate resorufin beta-d-galactopyranoside and a constant amount of the enzyme be

39 he coupled stoichiometric translocation of a galactopyranoside and an H(+) across the cytoplasmic mem

40 mic membrane protein, catalyzes symport of a galactopyranoside and an H(+) by using an alternating ac

41 nce of 2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside and Na(+), Li(+), or H(+), which impli

42 low and high salt buffers, of methyl beta-d-galactopyranoside and the methyl glycoside of beta(1-->6

43 sidue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by me

44 rate of acid-catalyzed hydrolysis of methyl galactopyranosides and of spontaneous hydrolysis of 2,4-

45 tosidase and its substrate (resorufin-beta-D-galactopyranoside) and found K(m) and k(cat) to be 333 +

46 xidation of 1-O-methyl-6, 6'-di-[2H]-alpha-d-galactopyranoside, and at high galactose concentrations,

47 This novel series of bicyclic galactopyranosides are prepared through a stereocontroll

48 ha and beta anomers of methyl D-gluco- and D-galactopyranoside as guests.

49 -consumption assays using 1-O-methyl-alpha-D-galactopyranoside as the sugar substrate to produce an a

50 Even for 3,4,6-O-unprotected galactopyranosides as triols, axial 4-O-acylation is app

51 tosidase and its substrate, resorufin-beta-D-galactopyranoside, as the model system of the enzyme rea

52 re identified as cholesteryl 6-O-acyl-beta-d-galactopyranoside (B. burgdorferi glycolipid 1, BbGL-I),

53 Using benzyl 2-acetamido-2-deoxy-alpha-d-galactopyranoside (BADG) to inhibit complete O-linked gl

54 beta(alpha)-d-galactopyranosyl-(1,1)-beta-d-galactopyranoside (betabeta-(Galp)(2)) and alphabeta-(Ga

55 Both alpha- and beta-methyl D-galactopyranosides bind more strongly than galactose, su

56 Nitro- or methyl-substituted phenyl alpha-D-galactopyranosides bind with significantly higher affini

57 Surprisingly, relative to methyl alpha-D-galactopyranoside, binding of cyclohexyl alpha-D-galacto

58 alactose (K(D) = 30 mM), while methyl beta-D-galactopyranoside binds only 3-fold better.

59 With wild-type LacY, binding of a galactopyranoside, but not a glucopyranoside, results in

60 identified as 7-O-methylcyanidin 3-O-beta-D-galactopyranoside by NMR spectroscopy.

61 anoside to give 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside causes (1) a change in the rate-determ

62 3 fusion protein was isopropyl-1-thio-beta-D-galactopyranoside concentration-, induction growth time-

63 action resulted in formation of azide beta-d-galactopyranoside, confirming Asp327 as the nucleophilic

64 d with release of fluorophenyl aglycons from galactopyranoside conjugates.

65 orimetric assay with chlorophenol red-beta-d-galactopyranoside (CPRG) for bacteria quantification.

66 the color substrate, chorophenol red-beta-D-galactopyranoside (CPRG), the signal from as few as 400

67 then incubated with chlorophenol red-beta-d-galactopyranoside (CPRG), which reacts with beta-gal to

68 nsor consists of the chlorophenol red-beta-D-galactopyranoside (CPRG)-loaded hyaluronic acid (HA) hyd

69 l was detected using chlorophenol red-beta-d-galactopyranoside (CRPG), a colorimetric substrate which

70 hloro-9,9-dimethylacridin-2-one-7-yl) beta-d-galactopyranoside (DDAOG), can be cleaved by beta-gal to

71 sugar (2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside) demonstrate that MelB-ST, in the pres

72 the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine

73 e, 6, 8-difluoro-4-methylumbelliferyl beta-d-galactopyranoside (DiFMUG), was found to be considerably

74 In contrast, the omega-angle in galactopyranosides displays a high proportion of the ant

75 However, methyl or allyl alpha-D-galactopyranosides exhibit 60-fold lower apparent K(d)'s

76 iously, methyl- or allyl-substituted alpha-D-galactopyranosides exhibit a 60-fold increase in binding

77 nchanged (K(D) = 0.4 mM), and phenyl alpha-D-galactopyranoside exhibits only a modest increase in bin

78 atalyzed hydrolysis of fluorescein di-beta-D-galactopyranoside (FDG), a commonly used fluorescent sub

79 or the hydrolysis of fluorescein mono-beta-d-galactopyranoside (FMG) by beta-d-galactosidase.

80 onvert the substrate fluorescein mono-beta-D-galactopyranoside (FMG) into fluorescein.

81 lected, induced with isopropyl-1-thio-beta-D-galactopyranoside for fusion gene expression and an expr

82 d hydrolysis of 4-nitrophenyl-2-deoxy-beta-d-galactopyranoside from breakdown to formation of the cov

83 cosides of 2-azido-2-deoxy-beta-d-gluco- and galactopyranosides from d-N-acetylglucosamine using a ca

84 spontaneous hydrolysis of 2,4-dinitrophenyl galactopyranosides has been studied through the synthesi

85 Recent studies using 1-O-methyl alpha-D-galactopyranoside have revealed an unusually large kinet

86 X-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) histochemical staining to detect the

87 ic hypoxic markers beta-D-iodinated azomycin galactopyranoside (IAZGP) and [99mTc]HL-91 were simultan

88 luate iodine 124 (124I)-labeled iodoazomycin galactopyranoside (IAZGP) positron emission tomography (

89 ip1 upon addition of isopropyl-1-thio-beta-D-galactopyranoside in the culture medium.

90 observations that hydrophobic aglycons of D-galactopyranosides increase binding affinity, with a cle

91 Addition of 5 mM isopropyl-1-thio-beta-D-galactopyranoside induced the expression of K-RasVal12,

92 Isopropyl-1-thio-beta-D-galactopyranoside-induced expression of p27Kip1 in these

93 ition, beta,D-galactopyranosyl 1-thio-beta,D-galactopyranoside induces a significant increase in cros

94 ed to stably express isopropyl-1-thio-beta-D-galactopyranoside-inducible Cox-2 (PCXII-4), we have inv

95 Isopropyl-1-thio-beta-d-galactopyranoside-inducible expression of exogenous Id-1

96 ignaling, we used an isopropyl-1-thio-beta-d-galactopyranoside-inducible expression system to express

97 An isopropyl-1-thio-beta-D-galactopyranoside-inducible P16 construct was introduced

98 ine lactones (AHLs) or isopropyl-beta-D-thio-galactopyranoside (IPTG) can be utilized for the impleme

99 the addition of 5 mM isopropyl-1-thio-beta-D-galactopyranoside (IPTG) induced the expression of Ha-Ra

100 n in which lrp is under isopropylthio-beta-D-galactopyranoside (IPTG)-inducible control.

101 nd is inducible with isopropyl-1-thio-beta-D-galactopyranoside (IPTG).

102 or hydrolysis of 2,2,2-trifluoroethyl beta-D-galactopyranoside is due entirely to a wild type enzyme

103 nding specificity in lactose permease toward galactopyranosides is governed by H-bonding interactions

104 e model and indicate that specificity toward galactopyranosides is governed by H-bonding interactions

105 possesses similar affinities for methyl beta-galactopyranoside, lactose, and N-acetyllactosamine and

106 Methyl beta-d-galactopyranoside malonyl aromatic esters have been desi

107 using 5-dodecanoylaminofluorescein di-beta-d-galactopyranoside, markers of tissue residence, and chem

108 comimetic featuring this rigidified bicyclic galactopyranoside moiety prevents neutrophil adhesion to

109 binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety.

110 cifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique sui

111 y used substrate 4-methylumbelliferyl beta-d-galactopyranoside (MUG), for the detection of beta-galac

112 valently bound inactivator suggests that the galactopyranoside must be fully ligated to induce an occ

113 hloro-9,9-dimethylacridin-2-one-7-yl} beta-D-galactopyranoside), noninvasive in vivo imaging of apopt

114 tructural analogues of p-nitrophenyl alpha-D-galactopyranoside (NPG) was examined by site-directed N-

115 cetylated octadecyl 1-thio-alpha- and beta-D-galactopyranosides observed by TEM could be rationalized

116 ose or beta-d-galactopyranosyl 1-thio-beta-d-galactopyranoside of approximately 1.0 mM or 40 microM,

117 The substrate 2-fluoro-4-nitrophenyl beta-D-galactopyranoside (OFPNPG) is readily hydrolyzed by beta

118 nzyme had activity towards o-Nitrophenyl-B-D-galactopyranoside (ONPG) and p-Nitrophenyl-B-D-fucopyran

119 e analyzed the entry of o-nitrophenyl-beta-D-galactopyranoside (ONPG) into cells induced to express V

120 s 2.8 and it hydrolysed o-nitrophenyl beta-d galactopyranoside (ONPG) with a K(m) value of 1.73 mM at

121 colorimetric substrate o-nitrophenyl-beta-d-galactopyranoside (ONPG), the signal-to-background ratio

122 The withdrawal of isopropyl-1-thio-beta-d-galactopyranoside or the addition of an inhibitor of the

123 ith X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) or by immunofluorescence with an anti

124 ectrochemical method with 4-aminophenyl-beta-galactopyranoside (PAPG) as a substrate.

125 beta-Galactosidase converts p-aminophenyl galactopyranoside (PAPG) to p-aminophenol (PAP).

126 ion of beta-galactosidase with p-aminophenyl-galactopyranoside (PAPG).

127 that the C-2, C-3, C-4, and C-6 OH groups of galactopyranosides participate in important H-bonding in

128 1.5+/-2.7% 5-bromo-4-chromo-3-indolyl-beta-D-galactopyranoside-positive cells and 81.1+/-3.6 U/g beta

129 eta-d-idopyranosyl configuration from beta-d-galactopyranosides, prior to a C-6-homologation extendin

130 analog beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside protects both lactose transport and en

131 mease, beta-D-galactopyranosyl 10thio-beta-D-galactopyranoside quenches the fluorescence of 2-(4'-mal

132 ing to alpha-D-galactopyranosyl-(1-4)-beta-D-galactopyranoside receptors in the kidneys.

133 The cyclic phosphate on the beta-galactopyranoside residue is in contact with the colitos

134 a-L-arabinopyranose residues than for beta-D-galactopyranoside residues and possessed a novel enzymat

135 on of p21(WAF1) with isopropyl-1-thio-beta-D-galactopyranoside restored this response.

136 molog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the

137 [4.4.0] dioxadecane-modified 3- O,4- C-fused galactopyranoside scaffold that locks the carboxylate ph

138 In the galactopyranoside series, beta-glycosides are formed sel

139 Kinetic studies using o-nitrophenyl-beta-D-galactopyranoside showed that BgaS with and without a Hi

140 We conclude that protonated LacY binds D-galactopyranosides specifically, inducing an occluded st

141 ermined by 5-bromo-4-chromo-3-indolyl-beta-D-galactopyranoside staining and beta-galactosidase assay

142 etected by 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside staining for Lac Z expressed by the vi

143 strated by 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside staining of cells infected by beta-gal

144 nitored by 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside staining of pancreatic tissue.

145 by X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) staining and Western blotting.

146 cal X-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining demonstrated expression of b

147 by X-Gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining.

148 ein, and the side chains shown to ligate the galactopyranoside strongly confirm more than two decades

149 Specifically, an indolyl galactopyranoside substrate was employed in conjunction

150 ity with the "native"para-nitrophenyl-beta-d-galactopyranoside substrate) were evolved in seven round

151 ining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside substrate, indicating expression of th

152 c myocytes using lipophilic fluorogenic beta-galactopyranoside substrates for real-time detection of

153 nce of beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside (TDG) or a proton electrochemical grad

154 ion of beta-d-galactopyranosyl 1-thio-beta-d-galactopyranoside (TDG), indicating that ligand induces

155 nce of beta-D-galactopyranosyl 1-thio-beta-D-galactopyranoside (TDG), lactose, or galactose at variou

156 M for beta, D-galactopyranosyl-1-thio-beta,D-galactopyranoside (TDG).

157 ch those of beta-D-(125)I-iodinated azomycin galactopyranoside, the optimal hypoxia marker of the azo

158 lyzes the hydrolysis of 4-nitrophenyl beta-D-galactopyranoside through a galactosyl-enzyme intermedia

159 an efficient strategy to synthesize bicyclic galactopyranosides through a key stereoselective equator

160 ning of this carbon atom in alpha- or beta-D-galactopyranosides thus may provide a rationale for the

161 C2-OH group by C2-H at 4-nitrophenyl-beta-d-galactopyranoside to give 4-nitrophenyl-2-deoxy-beta-d-g

162 ctopyranoside, binding of cyclohexyl alpha-D-galactopyranoside to lactose permease is essentially unc

163 Galactosidase converted p-aminophenyl beta-D-galactopyranoside to p-aminophenol (PAP).

164 reaction of nonfluorescent resorufin-beta-D-galactopyranoside to yield D-galactose and fluorescent r

165 Isopropyl-1-thio-beta-D-galactopyranoside-treated, arrested cells were significa

166 hydrolysis of 2-chloro-4-nitrophenyl-beta-D-galactopyranoside using UV-vis spectroscopy.

167 ccumulation of proline and thiomethyl-beta-D-galactopyranoside was partially restored to wild-type le

168 Kaempferol dirhamnosyl-galactopyranoside was the prevalent phenolic compound in

169 K(d)(app) for three different galactopyranosides was determined over a pH range from 5

170 L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside were compared, the K(m) values were 1.

171 vatives of beta-D glucopyranoside and beta-D-galactopyranoside were prepared by direct sulfation of t

172 pyranoside (alphaCG) and cholesteryl alpha-d-galactopyranoside were prepared in high yield.

173 p21(WAF1) induced by isopropyl-1-thio-beta-D-galactopyranoside were similar to those induced by NGF.

174 2-deoxy-3-O-beta-D-galactopyranosyl-alpha- D-galactopyranosides, were found to be effective chromogen

175 -2-deoxy-3-O-beta -D-galactopyranosyl-beta-D-galactopyranoside, which is a competitive inhibitor of t

176 Using fluorescein di-beta-d-galactopyranoside, which is a fluorogenic substrate for

177 St)) catalyzes the stoichiometric symport of galactopyranoside with a cation (H(+), Li(+), or Na(+))

178 In contrast, D-galactopyranosides with a variety of large hydrophobic s

179 In the present study, galactopyranosides with cyclohexyl or phenyl substitutio

180 Binding of alpha- and beta-D-galactopyranosides with different hydrophobic aglycons w

181 A new series of orally available alpha-d-galactopyranosides with high affinity and specificity to

182 bioluminogenic substrate, D-luciferin-O-beta-galactopyranoside, with short assay time and small volum

183 irst stained with 5-bromo-4-chloro-3-indolyl galactopyranoside (X-gal) as wholemounts and then sectio

184 substrate 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) at pH 6.0, a reaction conditio

185 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) staining demonstrated active t

186 tochemical 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) staining demonstrated amelobla

187 r positive 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside (X-Gal) staining.

188 lyzed with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor beta-galactosidase

189 ented with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor lacZ gene expressio

190 ining with 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-gal), a quantitative spectrophotome

191 ining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal), and the frequencies of inacti

192 ibution of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal)-stained cells was examined in

193 ical stain 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal).

194 mination of 5bromo-4-chloro-3-indolyl-beta-D-galactopyranoside- (X-gal) and halogenated indolyl-beta-