ライフサイエンスコーパス: gap-junctional

1 odel of primary sudden cardiac death defines gap junctional abnormalities as a key molecular feature

2 Decreased gap junctional and phosphorylated Cx43 was also detected

3 We study the effects of gap-junctional and ephaptic coupling on conduction in th

4 +) alternans that relies on a combination of gap-junctional and voltage-Ca(2+) coupling.

5 ional resistivity such that increased total, gap-junctional, and proportional Cx40 expression increas

6 6 K protein correlates with known changes in gap junctional area in the regenerating weanling rat liv

7 ultures were carried out and correlated with gap-junctional areas per inferface determined by freeze-

8 nductance; (iii) when applied to slices, the gap junctional blocker, 18 alpha-glycyrrhetinic acid, wh

9 increase in membrane stress, and blocked by gap junctional blockers.

10 cellular physical changes were prevented by gap-junctional blockers, oleamide and beta-glycyrrhetini

11 es in gap junctional channel gating, unitary gap junctional channel conductance, and hemichannel gati

12 ence of astrocytic Cx43 protein and that its gap junctional channel function is not necessary for Cx4

13 homology, they exhibit marked differences in gap junctional channel gating, unitary gap junctional ch

14 A gap junctional channel is formed by two hemichannels.

15 actors are required for the decrease in Cx43 gap-junctional channel permeability.

16 mBMSCs formed connexin 43 (Cx43)-containing gap junctional channels (GJCs) with the alveolar epithel

17 Our results show that Cx45.6 gap junctional channels are three times less permeable t

18 Previous studies have shown that gap junctional channels formed from the lens connexins C

19 rease was due to unregulated accumulation of gap junctional channels from nonjunctional pools, rather

20 e absolute permeabilities calculated for all gap junctional channels in this study are, with one exce

21 Hemi-gap junctional channels in Xenopus oocytes are formed fr

22 e action of at-RA, suggesting that gating of gap junctional channels is mediated through an RAR(beta)

23 nes from oocytes, and it is likely that hemi-gap junctional channels provide Ca2+ and metabolism-sens

24 The data indicate that hemi-gap junctional channels provide the Ca2+-inhibited pathw

25 oocytes injected with D47A cRNA did not form gap junctional channels when paired homotypically.

26 ing many of biophysical properties of Cx45.6 gap junctional channels, including molecular permeabilit

27 Unlike gap junctional channels, Panx1 forms single-membrane cha

28 Connexin (Cx) protein forms hemichannels and gap junctional channels, which play diverse and profound

29 educed the unitary conductance of Cx45.6-43N gap junctional channels.

30 nce, and decreased the conductance of single gap junctional channels.

31 dent on an extensive network of cell-to-cell gap junctional channels.

32 Gap-junctional channels (connexin oligomers) are large-d

33 that the permeability of connexin 43 (Cx43) gap-junctional channels (connexons) to small organic mol

34 (Cx43) by PKC abolishes the permeability of gap-junctional channels and hemichannels to large hydrop

35 Gap-junctional channels are formed by two connexons or g

36 rmeability, and which cannot form functional gap-junctional channels between neighboring cells.

37 ssed at the plasma membrane and did not form gap-junctional channels but formed hemichannels that beh

38 One of the most striking features of hemi-gap-junctional channels is that they are dramatically mo

39 The homotypic gap-junctional channels were closed in a voltage-depende

40 e-dependent manner, but failed to affect non-gap-junctional channels, including glutamate receptors.

41 Gap-junctional channels, permeable to large hydrophilic

42 s, transient receptor potential channels and gap-junctional channels.

43 ells, preventing the formation of functional gap-junctional channels.

44 the gap junctions (immunostaining), improved gap junctional communication (dye spread), and reduced v

45 molecular and functional characteristics of gap junctional communication (GJC) in the auditory and v

46 v-Src disrupts connexin (Cx)43 intercellular gap junctional communication (GJC) is not clear.

47 Gap junctional communication (GJC) modulates the express

48 en ECs, established Cx43-based intercellular gap junctional communication (GJC) with ECs, and increas

49 ng pathway responsible for the disruption of gap junctional communication (GJC), wild-type PDGF recep

50 several gap junction proteins suggests that gap junctional communication among motor neurons may be

51 Gap junctional communication and channel assembly can be

52 ynamic localization in frog embryos requires gap junctional communication and H,K-ATPase function.

53 ssion of connexin43 and connexin45 modulates gap junctional communication and production of bone matr

54 modulates coupling mediated by Cx50 and that gap junctional communication and signal transduction pat

55 Taken together, these results suggest that gap junctional communication and the Cx expression profi

56 involved in this developmental regulation of gap junctional communication are largely unknown.

57 neurons may maintain or even increase their gap junctional communication as they mature.

58 y guarding pHi, NHE1 preferentially protects gap junctional communication at intercalated discs, whil

59 the osteocalcin CxRE, and that disruption of gap junctional communication attenuates the ability of c

60 Src can phosphorylate Cx43 to block gap junctional communication between transformed cells.

61 18beta-glycyrrhetinic acid, blocked not only gap junctional communication but also intercellular calc

62 Importantly, disruption of gap junctional communication by overexpression of connex

63 This observation may help explain how gap junctional communication can be suppressed between m

64 Gap junctional communication had no apparent influence o

65 Thus, the reduction in gap junctional communication in Cx43 KO astrocytes leads

66 compatible with current models for a role of gap junctional communication in dorso-ventral patterning

67 lling mediated by protein kinase C (PKC) and gap junctional communication in TE differentiation and b

68 Therefore, gap junctional communication in the POA and hypothalamus

69 These data suggest the potential for gap junctional communication in the pre-BotC of both neo

70 connexins has underscored the importance of gap junctional communication in the skin and its appenda

71 In C. elegans, the role of gap junctional communication in touch sensitivity has be

72 ngs suggest that unique biochemical modes of gap junctional communication influence lens clarity and

73 estigate whether growth factor signaling and gap junctional communication interact during the develop

74 In the ocular lens, gap junctional communication is a key component of homeo

75 , there has been little evidence that direct gap junctional communication is a requirement for endocy

76 Gap junctional communication is essential for proper dev

77 Astrocytic gap junctional communication is important in steroid hor

78 Gap junctional communication is often lower between aggr

79 1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed.

80 Functional studies showed gap junctional communication is reduced and cell motilit

81 Here we evaluate whether gap junctional communication is required for the LR asym

82 Gap junctional communication is therefore both necessary

83 Thus, Src utilizes Cas to inhibit gap junctional communication mediated by Cx43.

84 We find that perturbing gap junctional communication not only slows the INM of p

85 +i cross gap junctions, so we tested whether gap junctional communication plays a role in changes in

86 nse alleles exhibiting high or low levels of gap junctional communication reveal a correlation betwee

87 PKCgamma mediated IGF-I-induced decreases in gap junctional communication through interaction with an

88 serine368 (S368) has been shown to decrease gap junctional communication via a reduction in unitary

89 Dye-coupling analysis indicated that gap junctional communication was inhibited in the cardia

90 However, significant gap junctional communication was not required, indicatin

91 Blockade of gap junctional communication with carbenoxolone did not

92 de responses being abolished by interrupting gap junctional communication with the connexin-mimetic p

93 ile flow in a manner that depends in part on gap junctional communication, even though the two hormon

94 chick embryos with lindane, which diminishes gap junctional communication, frequently unbiased normal

95 Inhibition of gap junctional communication, in contrast, had no influe

96 ompounds traditionally used as inhibitors of gap junctional communication, like heptanol and 18beta-g

97 32 form the neuroanatomic substrate for this gap junctional communication, which may be important in

98 l with respect to PKC-mediated signaling and gap junctional communication.

99 ill the scratch required early (within <6 h) gap junctional communication.

100 separate pathways for neuronal versus glial gap junctional communication.

101 orly invasive, possibly due to their loss of gap junctional communication.

102 shown to exert dominant negative effects on gap junctional communication.

103 d/or Ser282 initiates the down-regulation of gap junctional communication.

104 signaling cascade leading to enhancement of gap junctional communication.

105 f Cx43 to the plasma membrane, and inhibited gap junctional communication.

106 Following the hypothesis that gap-junctional communication (GJC) may underlie this sig

107 ccurrence in the hippocampus is dependent on gap-junctional communication and it has been suggested t

108 a oscillations has been suggested to involve gap-junctional communication between axons of principal

109 d meiotic maturation in part by antagonizing gap-junctional communication between sheath cells and oo

110 gap junctions, and that neurons can regulate gap-junctional communication by glia.

111 Suppression of gap-junctional communication by various protein kinases,

112 Therefore, the absence of gap-junctional communication caused by R75W expression i

113 Furthermore, we show for the first time that gap-junctional communication is an important requirement

114 This study demonstrates that heterologous gap-junctional communication is required for the complet

115 Changes in gap-junctional communication were asymmetrical, indicati

116 tic G alpha(o/i) and G alpha(s) pathways and gap-junctional communication with somatic cells of the g

117 art by antagonizing inhibitory sheath/oocyte gap-junctional communication.

118 iated by voltage-gated calcium signaling and gap-junctional communication.

119 ylate cyclase signaling and soma-to-germline gap-junctional communication.

120 sites of cell-cell contact, adjacent to the gap junctional complex.

121 alization of pH(i) from 7.2 to 7.8 increased gap junctional conductance (g(j)) of approximately 100-f

122 Gap junctional conductance (G(j)), and membrane conducta

123 It remains unknown whether changes in gap junctional conductance (Gj) accompany the increased

124 of the study was to determine the effect of gap junctional conductance (Gj) remodeling and Cx43 redi

125 ance (gCa(V)) using verapamil or by reducing gap junctional conductance (Gj) using carbenoxolone or b

126 Gap junctional conductance (Gj) was measured using a dua

127 er connexin43 (Cx43) overexpression enhanced gap junctional conductance (Gj).

128 mp recording techniques, we investigated the gap junctional conductance and the coupling coefficient

129 sibly including a role of ZO-1 in regulating gap junctional conductance at these highly modifiable el

130 At-RA did not act on gap junctional conductance by lowering [pH](i) or by inc

131 entrant circuits may result from a decreased gap junctional conductance consequent to an increase in

132 (at-RA) reversibly reduced the amplitude of gap junctional conductance in a dose-dependent manner, b

133 We evaluated gap junctional conductance in CKO ventricular pairs usin

134 l mechanisms for maintained propagation when gap junctional conductance is severely reduced.

135 Enhanced gap junctional conductance may be useful to promote the

136 Transjunctional voltage regulates cardiac gap junctional conductance, but the kinetics of inactiva

137 lated by conduction velocity restitution and gap junctional conductance.

138 nexons may contribute to the modification of gap junctional conductance.

139 ellular pathways resulting in an increase in gap junctional conductance.

140 cells expressing wild-type CX50 showed large gap junctional conductances and extensive transfer of ne

141 the interdependency of sodium, calcium, and gap junctional conductances to safeguard cardiac conduct

142 irs or N2A cells, and examined the resulting gap junctional conductances.

143 eeks, but there were offsetting increases in gap junctional conductances.

144 They also demonstrate that, at low gap-junctional conductivities, the accuracy of fully mac

145 upling increases propagation velocity at low gap junctional conductivity while it decreases propagati

146 the onset of locomotion promote the loss of gap junctional connections between developing motoneuron

147 These interactions include gap-junctional connections between inhibitory cells and

148 These changes were preceded by a loss of gap junctional connexin43 labeling and astrocytic prolif

149 t unexpected findings involving targeting of gap junctional connexins.

150 increased resting calcium levels and reduced gap junctional coupling among astrocytes, at concentrati

151 results suggest that the reestablishment of gap junctional coupling among axotomized adult motor neu

152 In rodent lumbar spinal cord, extensive gap junctional coupling among motor neurons innervating

153 NMDA antagonist, has been shown to maintain gap junctional coupling among motor neurons.

154 both synaptic mechanisms and maintenance of gap junctional coupling among neurons within the spinal

155 These observations demonstrate that H1 cell gap junctional coupling and thus D1 receptor activity ar

156 ling are dynamic and that cell-type-specific gap junctional coupling arises gradually during spinal c

157 Mefloquine, at 25 microM, blocked gap junctional coupling between interneurons in neocorti

158 oblasts by approximately 20 mV and increased gap junctional coupling between myofibroblasts and cardi

159 ents provided the first direct evidence that gap junctional coupling between neurons, specifically me

160 by a factor of 4 by physiological levels of gap junctional coupling between sister MCs at the same g

161 st the hypothesis that reduced spinal neuron gap junctional coupling decreases temporally correlated

162 cifer yellow dye spread demonstrated reduced gap junctional coupling in areas with Connexin 43 isofor

163 nnel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish

164 from both bipolar and amacrine cells and by gap junctional coupling involving ganglion cells.

165 Interneuronal gap junctional coupling is a hallmark of neural developm

166 intercellular geometry of connexin43 (Cx43) gap junctional coupling is key to coordinated spread of

167 d motor neurons suggested that, after birth, gap junctional coupling is spatially restricted.

168 After injury, interneuronal gap junctional coupling may mediate signaling that maint

169 Our work shows that gap junctional coupling modulates neuronal activity patt

170 In the lens, Cx46 and Cx50 provide the gap junctional coupling needed for homeostasis and growt

171 amined the effect of light adaptation on the gap junctional coupling of alpha-ganglion cells (alpha-G

172 continuous subthreshold oscillations require gap junctional coupling of IO neuron somata within 40 mi

173 ugh the ERK pathway lead to the asymmetry in gap junctional coupling required for proper lens functio

174 ion, they suggest a novel mitogenic role for gap junctional coupling that is connexin specific and in

175 In testing the possible involvement of gap junctional coupling the following experimental obser

176 Gap junctional coupling was demonstrated by the presence

177 The relevance of heterocellular gap junctional coupling was evaluated by the use of fibr

178 Gap junctional coupling was stronger between astrocytes

179 uction velocity is relatively insensitive to gap junctional coupling when sodium ion channels are loc

180 n activity, which, in part, is controlled by gap junctional coupling within the spinal cord, appears

181 ffects of changes in extracellular geometry, gap junctional coupling, and sodium ion channel distribu

182 erneurons was not related to the strength of gap junctional coupling, and was still prevalent in conn

183 slet cells, are believed to arise in part by gap junctional coupling, but the mechanisms through whic

184 t mechanisms, particularly those mediated by gap junctional coupling, regulate synapse formation with

185 The effect of gap junctional coupling, sodium ion channel distribution

186 e potentials and cardiomyocyte-cardiomyocyte gap junctional coupling, TGF-beta1 depolarized cardiomyo

187 measurements revealed normal levels of ionic gap junctional coupling, whereas the passage of fluoresc

188 afficking of wild-type connexin36 and blocks gap junctional coupling.

189 f six of the chimeras induced high levels of gap junctional coupling.

190 urones were identified as potential sites of gap junctional coupling.

191 n event necessary and sufficient to increase gap junctional coupling.

192 by ephaptic effects, even in the absence of gap junctional coupling.

193 embrane potential oscillations suggestive of gap junctional coupling.

194 at earlier stages, though without involving gap junctional coupling.

195 in an electrically coupled network depend on gap junctional coupling?

196 Furthermore, both gap-junctional coupling and changes in [Ca(2+)](i) have

197 Gap-junctional coupling and gap junction-dependent Ca(2+

198 In addition to the often-described gap-junctional coupling between astrocytes, coupling als

199 We here show that Cx43 mediates gap-junctional coupling between collectively invading br

200 We show further that strong gap-junctional coupling of both membrane potential and c

201 We investigated gap-junctional coupling of rods and cones in macaque ret

202 al mice, and that patterning is modulated by gap-junctional coupling.

203 Both CX50 and CX50G46V induced gap junctional currents in pairs of Xenopus oocytes.

204 by its equivalent action on whole-cell hemi-gap-junctional currents and on cell-free excised patch h

205 on macroscopic human connexin46 (hCx46) hemi-gap-junctional currents using the two-electrode voltage-

206 is more stable and contains a high level of gap-junctional Cx43 whereas that formed with hypoxic cel

207 hypoxic melanoma cells selectively degrades gap-junctional Cx43, leading to the destabilization of t

208 le and contains a significant lower level of gap-junctional Cx43.

209 found hearing loss, but the mechanism of the gap-junctional defect is unknown.

210 eticular nucleus (TRN), a brain area rich in gap junctional (electrical) synapses, regulates cortical

211 eticular nucleus (TRN), a brain area rich in gap-junctional (electrical) synapses, regulates cortical

212 The synaptic connections, both chemical and gap junctional, form a neural network with four striking

213 sing SNARF as a proton carrier, suggest that gap junctional H+ transmission may be accomplished physi

214 Cx46 hemichannel opening, then dye influx by gap junctional/hemichannel permeable dyes should be meas

215 We propose that Zpg-containing gap junctional hemichannels in the germ cell plasma memb

216 Gap junctional hemichannels mediate cell-extracellular c

217 utamate release was not affected by blocking gap junctional hemichannels with 18alpha-glycyrrhetinic

218 Neuronal gap junctional hemichannels, composed of pannexin-1 subu

219 onal channels are formed by two connexons or gap-junctional hemichannels in series, with each connexo

220 Here, we show that Cx26 R75W forms gap-junctional hemichannels that display altered voltage

221 cium-dependent gating properties of two lens gap-junctional hemichannels were compared at the macrosc

222 es formed by head-to-head association of two gap-junctional hemichannels, one from each of the adjace

223 heterogeneous cells might be facilitated by gap junctional intercellular channels which are permeabl

224 Adenosine and NECA also stimulated gap junctional intercellular communication (as assessed

225 Disruption of gap junctional intercellular communication (GJIC) and/or

226 Gap junctional intercellular communication (GJIC) betwee

227 Loss of gap junctional intercellular communication (GJIC) betwee

228 nhibitor specific to Cx43, we show that Cx43-gap junctional intercellular communication (GJIC) influe

229 Gap junctional intercellular communication (GJIC) is wid

230 Gap junctional intercellular communication (GJIC) plays

231 the mixture of DMSO and PMA, an inhibitor of gap junctional intercellular communication (GJIC).

232 initial repair process that is dependent on gap junctional intercellular communication (GJIC).

233 We hypothesize that the level of gap junctional intercellular communication among dividin

234 elieved to be critical for the regulation of gap junctional intercellular communication and for the f

235 atinocytes, despite a lack of heterocellular gap junctional intercellular communication between the t

236 A rigorous and unambiguous demonstration of gap junctional intercellular communication demands both

237 y was used to quantitatively investigate the gap junctional intercellular communication in several ho

238 These data suggest that altered gap junctional intercellular communication may underlie

239 Gap junctional intercellular communication via these cha

240 suppression of networks for tight junction, gap junctional intercellular communication, and transmem

241 ifferences in gap junction plaque formation, gap junctional intercellular communication, Cx43 phospho

242 the number of neighboring cells coupled via gap junctional intercellular communication, providing a

243 sible mechanism for bilateral communication, gap junctional intercellular communication, was also inv

244 asal levels of Cx26 and Cx43, rendering them gap junctional intercellular communication-deficient as

245 ons dock between adjacent cells to allow for gap junctional intercellular communication.

246 valent number of MSCs was down-regulated via gap junctional intercellular communication.

247 rat osteocalcin promoter for sensitivity to gap junctional intercellular communication.

248 Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication.

249 t fibroblast growth factor (FGF) upregulates gap junctional intercellular dye transfer in primary cul

250 rovide a valuable model to study the role of gap-junctional intercellular communication in epidermal

251 ay and Western blot analyses reveal that the gap junctional intracellular communication molecule conn

252 Gap junctional mechanisms require further consideration

253 Once engaged with the astrocyte gap-junctional network, brain metastatic cancer cells us

254 xin47 (Cx47) and astrocytic Cx30 to panglial gap junctional networks as well as myelin maintenance an

255 may have emerged via restoration of previous gap-junctional networks.

256 mulations of these models revealed that this gap junctional/paracrine mechanism accounts for up to 23

257 but are consistent with the hypothesis that gap-junctional particles contain central hydrophilic cha

258 ermits a determination of absolute values of gap junctional permeability as a function of dye size an

259 Thus, altering gap junctional permeability by manipulating the expressi

260 ctation, H(+) ions can induce an increase of gap-junctional permeability.

261 Cx26 and Cx30 were also coexpressed in the gap junctional plaques between Claudius cells.

262 ly different from hemichannels in the docked gap junctional plaques examined after their exposure by

263 e was reduced and incorporation of Cx43 into gap junctional plaques was markedly diminished.

264 vere reductions in function and formation of gap junctional plaques were transferred to other connexi

265 lian cells, the mutant CX46 was not found at gap junctional plaques, but it showed extensive co-local

266 ression or relocation to the cell surface or gap junctional plaques.

267 ible promoter, both CX50 and CX50G46V formed gap junctional plaques.

268 Our results further show that gap-junctional plaques formed of a sorting motif-deficie

269 r intercellular transport and communication, gap junctional pores have been the subject of numerous i

270 The gap junctional protein connexin (Cx) 45 is strongly expr

271 The contribution of the gap junctional protein connexin 36 (Cx36) to the regulat

272 The pore-forming gap junctional protein connexin 43 (Cx43) has a short (1

273 5, integrin alpha3beta1, E-cadherin, and the gap junctional protein connexin 43.

274 Expression of the redox-sensitive gap junctional protein Cx43 (Connexin 43) was reduced in

275 onal circuit, by increasing targeting of the gap junctional protein innexin shaking-B to gap junction

276 iol and progesterone on the abundance of the gap junctional protein, connexin 43 (CX43), which is hig

277 d disruption of GJC and phosphorylation of a gap junctional protein, connexin-43 (Cx43), which requir

278 ed knockout of Connexin43, the major cardiac gap junctional protein, uniformly develop sudden cardiac

279 Because gap junctional proteins are widely expressed in the mamm

280 dentified in vertebrates and found to encode gap junctional proteins as well.

281 ndertaken to examine the hypothesis that the gap junctional proteins connexin 32 (Cx32; expressed by

282 constructed a series of chimeric Cx46-Cx45.6 gap junctional proteins in which a single transmembrane

283 Two gap junctional proteins, connexin43 and connexin45, were

284 o re-entry) that could arise from changes in gap junctional proteins, especially connexin43 (Cx43).

285 the surface expression of unique subsets of gap junctional proteins.

286 e increase in the volume of cells expressing gap-junctional proteins (connexins).

287 The relative roles of the gap-junctional proteins connexin40 (Cx40) and connexin43

288 ocity (theta) and anisotropic ratio, and how gap junctional remodeling is modulated by the extracellu

289 el that exhibits ventricular arrhythmias and gap junctional remodeling, beta-catenin and Cx43 express

290 In infarcted hearts, adverse gap-junctional remodeling occurs in the border zone, whe

291 s in cEC transmembrane current densities and gap junctional resistances can affect K(+)-mediated NVC

292 Specific gap-junctional resistivity (Rj) correlated not only with

293 nvestigated by direct ex vivo measurement of gap-junctional resistivity and quantitative connexin imm

294 Cx40 is associated with human right atrial gap-junctional resistivity such that increased total, ga

295 0 expression and a corresponding increase in gap-junctional resistivity.

296 , and proportional Cx40 expression increases gap-junctional resistivity.

297 As such, gap junctional signalling appears to be a substantial co

298 Cx43 within the plasma membrane at apparent gap junctional structures.

299 ted a slight increase in total Cx43, whereas gap junctional (Triton-insoluble) Cx43 decreased, and no

300 led to myofibroblasts during pharmacological gap junctional uncoupling.