ライフサイエンスコーパス: gel filtration

1 ngth, detergent-solubilized EGFR as shown by gel filtration.

2 nalytical ultracentrifugation and analytical gel filtration.

3 retained their dimeric aggregation state on gel filtration.

4 ays, surface plasmon resonance analysis, and gel filtration.

5 t species of m.w. 1,400,000 were observed by gel filtration.

6 complex (5NT, Galpha(s), and Gbetagamma) by gel filtration.

7 presence of soluble oligomers observable by gel filtration.

8 milk and various milk fractions obtained by gel filtration.

9 parated into four major fractions (F1-F4) by gel filtration.

10 n during folding in solution was observed by gel filtration.

11 d HeLa cells using column chromatography and gel filtration.

12 with both hemin and protoporphyrin IX during gel filtration.

13 nm large disks was detected and isolated by gel filtration.

14 graphy, His-Trap affinity chromatography and gel filtration.

15 ex with 1 beta-tryptase monomer and 1 Fab by gel filtration.

16 ation and other repair proteins, as shown by gel filtration.

17 r, in agreement with results from analytical gel filtration.

18 e chromatography followed by Sephacryl S-300 gel filtration.

19 the MUC2-N trimer eluted as a single peak by gel filtration.

20 d the pentameric structure, as determined by gel filtration, 1-anilinonaphthalene-8-sulfonic acid-bin

21 ied by fractionation with ammonium sulphate, gel filtration, affinity and ion exchange chromatography

22 Gel filtration, affinity selection and mass spectrometry

23 High performance liquid chromatography gel filtration analyses of this mini-spectrin provide th

24 Gel filtration analyses show that unphosphorylated DUE-B

25 We show in velocity sedimentation and gel filtration analyses that the mitochondrial DNA helic

26 We used gel filtration analyses to analyze the size of oligomers

27 Confocal microscopy and gel filtration analyses were performed to assess their p

28 Gel filtration analyses, analytical ultracentrifugation

29 Using immunoprecipitation and gel filtration analyses, we found that Holliday junction

30 D-1 ligand by native gel electrophoresis and gel filtration analyses.

31 inity purification, immunoprecipitation, and gel filtration analyses.

32 Gel filtration analysis and blue native gels showed that

33 Gel filtration analysis and cross-linking experiments in

34 Gel filtration analysis of the alphaL260P mini-spectrin

35 Here we use gel filtration analysis of the cytosol of primary and es

36 of NOD2 with another NLR protein, NALP1, and gel filtration analysis revealed the formation of a comp

37 t phosphorylation enhances PKR dimerization, gel filtration analysis reveals a second monomeric phosp

38 concentration-dependent self-association in gel filtration analysis.

39 ex and partially cofractionates with p300 by gel filtration analysis.

40 odeling factor Mi2, were found to coelute by gel-filtration analysis and pull-down assays.

41 Circular dichroism and gel-filtration analysis indicated an extended, unstructu

42 Here we show by gel filtration, analytical ultracentrifugation, and NMR

43 show using a variety of techniques including gel-filtration, analytical ultracentrifugation, electron

44 The gel filtration and 2D gel electrophoresis analysis showe

45 haracterized their oligomerization states by gel filtration and analytical ultracentrifugation experi

46 imer-of-dimers association in agreement with gel filtration and analytical ultracentrifugation studie

47 Gel filtration and analytical ultracentrifugation studie

48 omplex, we undertook an in vitro study using gel filtration and analytical ultracentrifugation.

49 urified in the presence of Nonidet P-40 with gel filtration and anion exchange chromatography.

50 In vivo soluble Cdt1 and PCNA co-elute by gel filtration and associate with each other physically.

51 Gel filtration and biosensor binding experiments reveale

52 Gel filtration and blue native electrophoresis suggest s

53 precipitation or consecutive purification by gel filtration and blue native gel electrophoresis, we d

54 with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography.

55 cturally folded proteins, as demonstrated by gel filtration and circular dichroism.

56 Analytical gel filtration and covalent cross-linking of purified re

57 Surprisingly, gel filtration and cross-linking analysis showed that a

58 Gel filtration and cross-linking experiments demonstrate

59 Gel filtration and cross-linking of a 71-amino acid frag

60 In RAO-3 cells, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatog

61 LPPS was purified using Sepharose 6B gel filtration and dissolved into two major components,

62 adopt an open conformation, as determined by gel filtration and dynamic light scattering.

63 Analytical gel filtration and electrophoretic mobility shift assays

64 Gel filtration and immunoprecipitation assays show that

65 tear protein-FODE complexes were isolated by gel filtration and ion exchange chromatographies, monito

66 overy using ammonium sulphate precipitation, gel filtration and ion exchange chromatography.

67 n solution and inside cells using analytical gel filtration and luciferase complementation assays and

68 tudied by a combination of western blotting, gel filtration and mass spectrometry.

69 one-target interactions, in combination with gel filtration and molecular dynamics simulations, we he

70 was coeluted with P798-FX cores on both G-75 gel filtration and Ni affinity columns.

71 Importin alpha1 (Impalpha1) was confirmed by gel filtration and NMR studies.

72 S from the mesophile Methanosarcina mazei by gel filtration and nondenaturing mass spectrometry shows

73 Because of concurrent advances in gel filtration and other methods of protein separation,

74 ied as monomer, dimer, and oligomer forms by gel filtration and protein cross-linking.

75 complex was demonstrated by pulldown assays, gel filtration and proximity-dependent biotinylation.

76 nsLTP1 was purified using the combination of gel filtration and reverse-phase high-performance liquid

77 Azemiops feae viper venom by combination of gel filtration and reverse-phase HPLC.

78 The epitope off-rate was evaluated by gel filtration and T cell-based assays.

79 Gel filtration and UV spectroscopy reveal that MF1 exist

80 ere characterized by electron microscopy and gel filtration and were found to include annular species

81 crude cell lysates and from fractions after gel filtration and with baculovirally expressed proteins

82 rms through biochemical methods that include gel-filtration and native gel analysis as well as direct

83 Analytical gel-filtration and ultracentrifugation measurements conf

84 Native gel electrophoresis, gel filtration, and analytical ultracentrifugation demon

85 Site-directed mutagenesis, gel filtration, and analytical ultracentrifugation indic

86 ), surface plasmon resonance, cross-linking, gel filtration, and analytical ultracentrifugation studi

87 Using pull-down, gel filtration, and analytical ultracentrifugation studi

88 using NMR, isothermal titration calorimetry, gel filtration, and analytical ultracentrifugation.

89 purified by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography.

90 ance spectroscopy, dynamic light scattering, gel filtration, and autophosphorylation kinetics.

91 Kinetic analysis, analytical gel filtration, and chemical cross-linking demonstrated

92 Using NMR spectroscopy, gel filtration, and chemical cross-linking, we obtained

93 as shown by blue native gel electrophoresis, gel filtration, and determination of intermolecular dist

94 Physicochemical analyses using native gels, gel filtration, and differential scanning fluorimetry re

95 acterized by analytical ultracentrifugation, gel filtration, and electron microscopy.

96 Freshly dissolved EMD was fractionated by gel filtration, and forty-five 7-ml fractions were colle

97 ic by confocal microscopy, western blotting, gel filtration, and immunoprecipitation studies in macro

98 After purification by affinity, gel filtration, and ion exchange chromatography, NR3A S1

99 Using bacterial two-hybrid, gel filtration, and MS analyses, we demonstrate that SvW

100 for iNOS, using rapid stopped-flow kinetic, gel filtration, and spectrophotometric analysis.

101 e native polyacrylamide gel electrophoresis, gel filtration, and transmission electron microscopy of

102 ed using ammonium sulphate precipitation and gel-filtration, and subjected to multiple enzymatic dige

103 tly bound protein factors can be purified by gel filtration as a functional entity called the transcr

104 n assays confirmed their interactions, while gel filtration assay indicated that C53/LZAP and RCAD ma

105 Indeed, gel filtration assays demonstrated that although nucleot

106 s insertion into the PDZ binding pocket, and gel filtration assays showed that this phosphomimic muta

107 Mutagenesis combined with gel filtration assays suggested that the side chain hydr

108 by sucrose-loaded vesicle sedimentation and gel filtration assays, and the effects of pH and lipid c

109 Using co-immunoprecipitation and gel filtration assays, we show that CPEB specifically in

110 When examined by gel filtration at 0.5 M NaCl in the absence of DNA, Rep6

111 ry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, while its nat

112 rm rapidly and are stable in solution during gel filtration at low pH.

113 g assay and scintillation proximity assay or gel filtration binding assays using (3)H-labeled PPARalp

114 aracterization of cVIMP-Cys using analytical gel filtration, CD and NMR spectroscopy in conjunction w

115 ing only of the repeat-containing regions by gel filtration, CD spectroscopy, and negative-staining e

116 Using gel filtration, chemical cross-linking, analytical ultra

117 In gel filtration chromatography analyses of BC-3 cell lysa

118 inary approach, including live cell imaging, gel filtration chromatography analysis, split ubiquitin

119 TS co-migrated exclusively with USP9X during gel filtration chromatography analysis.

120 Gel filtration chromatography and analytical ultracentri

121 Analyses by gel filtration chromatography and analytical ultracentri

122 Using a combination of gel filtration chromatography and analytical ultracentri

123 Tear proteins were separated by gel filtration chromatography and analyzed for bound lip

124 Gel filtration chromatography and co-immunoprecipitation

125 mbination of ion-exchange chromatography and gel filtration chromatography and demonstrated for the f

126 Supplemented with gel filtration chromatography and fluorescence-adapted s

127 Both enzymes were found to be homodimeric by gel filtration chromatography and homotetrameric by dyna

128 Second, gel filtration chromatography and immunoprecipitation in

129 Using gel filtration chromatography and isothermal titration c

130 Both gel filtration chromatography and mass analysis from two

131 dimer to a monomeric protein on the basis of gel filtration chromatography and multiple-angle laser l

132 Using gel filtration chromatography and negative stain electro

133 on procedures including membrane filtration, gel filtration chromatography and reversed-phase high-pe

134 Gel filtration chromatography and SDS-PAGE revealed that

135 Analytical gel filtration chromatography and sedimentation velocity

136 tration membranes was further purified using gel filtration chromatography and two steps of reverse-p

137 nuclein was quantified by the combination of Gel filtration chromatography and Western blot, respecti

138 Purification through gel filtration chromatography confirmed the formation of

139 Gel filtration chromatography confirms the differentiati

140 ulldown assays of a Orai1-CMBD(W76E) mutant, gel filtration chromatography data, and NOE signals indi

141 Analytical gel filtration chromatography demonstrates that Slit D2

142 Gel filtration chromatography demonstrates that upon bin

143 Fractionation of plasma by gel filtration chromatography followed by bioassays indi

144 The resolving exudate was subjected to gel filtration chromatography followed by proteomics, id

145 se peptides were purified after affinity and gel filtration chromatography from a chickpea protein hy

146 0 Da, relative to globular standards, during gel filtration chromatography in the absence of polyanio

147 Fractionation of serum by gel filtration chromatography led to the identification

148 Gel filtration chromatography of conditioned medium from

149 In the present studies, gel filtration chromatography of liver cytosol from LFAB

150 During gel filtration chromatography of solubilized SR membrane

151 Gel filtration chromatography of the full-length fusion

152 olysis, circular dichroism spectroscopy, and gel filtration chromatography revealed that the N-termin

153 In addition, gel filtration chromatography separated multimeric and m

154 variant, we used chemical cross-linking and gel filtration chromatography to show that each exists a

155 C5 was demonstrated by Western blotting and gel filtration chromatography using 125I-labeled protein

156 s resolved from the excision activity during gel filtration chromatography using Sephacryl S-300.

157 hromatography using DEAE-Toyopearl 650 M and gel filtration chromatography using Sephadex G-100.

158 n-dodecyl beta-D-maltoside (DDM) examined by gel filtration chromatography was generally modest, and

159 rate the potential for real-time monitoring, gel filtration chromatography was used to separate diffe

160 crylamide gel electrophoresis and analytical gel filtration chromatography were used to detect the di

161 5 kD fraction (Ara h 2/6; 20 kD fraction) on gel filtration chromatography, account for the majority

162 , we used a combination of NMR spectroscopy, gel filtration chromatography, and analytical ultracentr

163 Using native gel electrophoresis, gel filtration chromatography, and surface plasmon reson

164 rm agglutinin chromatography, Sephacryl S300 gel filtration chromatography, anti-FLAG immunoaffinity

165 As determined by gel filtration chromatography, cGMP binding caused elong

166 Using a combination of gel filtration chromatography, differential scanning cal

167 med structural studies of this protein using gel filtration chromatography, intrinsic tryptophan fluo

168 ion bodies, but when subjected to analytical gel filtration chromatography, it elutes in the void vol

169 Here, using gel filtration chromatography, sedimentation, fluorescen

170 We have used gel filtration chromatography, site-directed mutagenesis

171 ry activity was analysed using Sephadex G-75 gel filtration chromatography, Superdex peptide 10/300 G

172 ecombinant protein expression and analytical gel filtration chromatography, surface plasmon resonance

173 Using gel filtration chromatography, we assessed the effects o

174 s, PCNA-coupled affinity beads pull-down and gel filtration chromatography, we show that the same reg

175 measurements below the polymer's LCST using gel filtration chromatography.

176 metal affinity, hydrophobic interaction, and gel filtration chromatography.

177 ueous hydrogen fluoride (HF) and purified by gel filtration chromatography.

178 roteins were purified by Ni-NTA affinity and gel filtration chromatography.

179 was stable during affinity purification and gel filtration chromatography.

180 apientum (MSP) were isolated and purified by gel filtration chromatography.

181 exchange chromatography, ultrafiltration and gel filtration chromatography.

182 fied using ultrafiltration, ion exchange and gel filtration chromatography.

183 The hydrolysate was further purified by gel filtration chromatography.

184 pitation with ammonium sulphate, followed by gel filtration chromatography.

185 ein still elutes in the void upon analytical gel filtration chromatography.

186 led under aprotic conditions and purified by gel-filtration chromatography (GFC) using high-specific-

187 Gel-filtration chromatography and analytical ultracentri

188 e N-terminal region that was investigated by gel-filtration chromatography and CD spectroscopy.

189 A combination of gel-filtration chromatography and circular dichroism spe

190 A combination of gel-filtration chromatography and protein overlay assays

191 Analytical gel-filtration chromatography confirms that the OMP.OPRT

192 of PDE3A2 by PKC led to shifts in elution on gel-filtration chromatography consistent with increased

193 Gel-filtration chromatography demonstrated an interactio

194 Gel-filtration chromatography demonstrated that ASRT for

195 However, examination of P-TEFb complexes by gel-filtration chromatography showed that TCR signaling

196 ass spectrometry, co-immunoprecipitation and gel-filtration chromatography to define higher-order pro

197 Plasma was fractionated using gel-filtration chromatography, and lipid-associated prot

198 t molecular mass of 110 kDa as determined by gel-filtration chromatography, indicating that the prote

199 We characterized NABBs by gel-filtration chromatography, native polyacrylamide gra

200 Bioactive peptide separated by gel-filtration chromatography, showed the higher antioxi

201 ated with high density lipoproteins (HDL) by gel-filtration chromatography, we found no evidence that

202 Using sequential size-exclusion and gel-filtration chromatography, we identified a condition

203 ricted to oligomer-containing fractions from gel-filtration chromatography.

204 dentical subunits) that the complex survives gel-filtration chromatography.

205 orresponding to approximately 170-180 kDa by gel-filtration chromatography.

206 Both antigens were compared by gel filtration, circular dichroism measurement, in vitro

207 rmed large aggregates that did not enter the gel filtration column but were made visible after densit

208 Our gel filtration column chromatography and cross-linking s

209 LpxL migration on a gel filtration column is consistent with a molecular mas

210 gent-solubilized protein migration through a gel filtration column toward smaller molecular masses wi

211 The factor eluted from the gel filtration column with an apparent molecular mass of

212 lowed by mass spectrometry, coelution from a gel filtration column, cosedimentation on a glycerol gra

213 MGAT2 also co-eluted with DGAT1 on a gel filtration column, suggesting that the two enzymes f

214 using a cation exchange column followed by a gel filtration column.

215 h-performance frontal analysis using a short gel-filtration column and phosphate buffered saline solu

216 ant larger liposomes are passed over another gel-filtration column to exchange an extraliposomal high

217 All the collected effluent from the gel-filtration column was then transferred to the second

218 nd chromatography through DEAE-Sepharose and gel filtration columns.

219 Furthermore, ABCG5/G8 eluted as a dimer on gel filtration columns.

220 Gel filtration confirmed that purified E. aediculatus te

221 Light scattering coupled to gel filtration confirms the monomeric state of solubiliz

222 ative state of alpha-synuclein was probed by gel filtration coupled with native gradient gel separati

223 with high-performance liquid chromatography gel filtration data and demonstrate that single-molecule

224 versible thermal denaturation and analytical gel filtration data suggest that the central CCM-1 is cr

225 Gel filtration demonstrated that the N1347S mutation dis

226 etory pathway we analyzed purified MUC2-N by gel filtration, density gradient centrifugation, and tra

227 assays) and multimerization (as revealed by gel filtration, dynamic light scattering, and analytical

228 as judged by analytical ultracentrifugation, gel filtration, dynamic light scattering, and CD.

229 DS resistance assay, antibody binding assay, gel filtration, dynamic light scattering, small angle x-

230 The gel filtration elution profile and the small angle x-ray

231 Gel filtration experiments indicate that MtATP-phosphori

232 Gel filtration experiments reveal that gC1qR clusters FX

233 t lacks an active site cysteine residue, and gel filtration experiments show that it resides in a hig

234 kably, gel shift, chemical cross-linking and gel filtration experiments showed that S326C both exists

235 Gel filtration experiments using wild-type and mutated X

236 rescence binding, X-ray crystallography, and gel filtration experiments with asparagine, aspartate, a

237 mers in vitro, as shown in cross-linking and gel filtration experiments.

238 lexes, as judged by bacterial monohybrid and gel filtration experiments.

239 ically from pooled stimulated human tears by gel filtration fast performance liquid chromatography.

240 and ORRM1 were shown to be present in active gel filtration fractions, though OZ1 and ORRM1 were main

241 olines between m/z 490 and 540 in any of the gel-filtration fractions.

242 binds tightly to human platelets even after gel filtration, has a prolonged half-life in mice transg

243 ically active EGFR dimers can be isolated by gel filtration in dodecyl maltoside.

244 the two form a complex, further suggested by gel filtration, in which the HMGBs with core histones el

245 However, as gel filtration indicated sufficient heterodimer was pres

246 Gel filtration indicated that the mutations did not affe

247 Analytical gel filtration indicated that the protein exists in solu

248 Analysis of the mutants by gel filtration indicates that at least five residues mus

249 Gel filtration indicates that the protein is not a dimer

250 we report on chromatographic fractionation (gel filtration, ion exchange, and hydroxyapatite) of ext

251 We used gel retardation, gel filtration, isothermal titration calorimetry and NMR

252 Pulldown assays, gel filtration, isothermal titration calorimetry, and ra

253 methods, including native gel and analytical gel filtration, isothermal titration calorimetry, NMR sp

254 characteristics of collagen, we developed a gel filtration-like procedure that uses columns containi

255 FOS was purified in a single step using gel filtration matrix, Bio-Gel P2.

256 sD readily formed a complex that elutes from gel filtration medium as a single included peak.

257 Here, we developed a simple gel filtration method to enrich semiconducting (12,1) an

258 Using novel flow cytometry and gel filtration methods, we quantified SS1P uptake in ind

259 Gel filtration, native PAGE, and protein cross-linking w

260 Gel filtration of HPAEC-CM revealed a peak of eosinophil

261 We performed gel filtration of liver supernatants and identified two

262 Analytical gel filtration of purified protein and cryo-electron mic

263 hown by isothermal titration calorimetry and gel filtration of recombinant subunits.

264 Gel filtration of the affinity-purified material further

265 Interestingly, gel filtration of this substrate-binding mutant also det

266 hange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75.

267 er various conditions has been studied using gel filtration or analytical ultracentrifugation experim

268 ntly removed from protein-lipid complexes by gel filtration or dialysis into high potassium (high [K+

269 w from a control strain were fractionated by gel filtration, outer arm dynein components were present

270 gomers were characterized concerning size by gel filtration, polyacrylamide gel electrophoresis, and

271 tural information of eluted materials in the gel filtration provides important clues about their beha

272 odimer of 28 kDa subunits on Superdex HR 200 gel filtration resin.

273 mers and dimers in solution, and equilibrium gel filtration revealed a 2:1 receptor/ligand binding st

274 Fractionation of rLcrV preparations via gel filtration revealed that only a minor component cons

275 Using analytical gel filtration, sedimentation-velocity ultracentrifugati

276 C), 80% ammonium sulfate precipitation, and gel filtration (Sephadex G25).

277 Chemical cross-linking and gel-filtration show that a 1:1 linker histone/HMGB1 comp

278 se partially purified by ultrafiltration and gel filtration showed a specific activity of 2793 U/mg.

279 Furthermore, sedimentation velocity and gel filtration showed that NEMO ZF, like other NEMO rela

280 Gel filtration showed that the open channel conformers t

281 haracterized the allosteric inhibition using gel filtration, steady-state and pre-steady-state kineti

282 ution, typically through a final dialysis or gel filtration step.

283 hift assays and footprinting, cross-linking, gel filtration stoichiometry, and DNA bending assays.

284 Circular dichroism spectroscopy and gel filtration studies confirm that this region has a he

285 Gel filtration studies indicated that 7B2 exists as an e

286 In addition, gel-filtration studies showed that formation of chaperon

287 and endogenous proteins and co-elution with gel filtration suggest that an endogenous Ajuba.Gfi1.HDA

288 Gel filtration suggested that DeltaKal7, a natural isofo

289 han the inactive holoenzyme when analyzed by gel filtration, suggesting an expanded conformation.

290 ar weight ATPase-DNA complex was isolated by gel filtration, suggesting oligomerization of ATPase fol

291 Gel filtration suggests that FDM1 may exist as a homodim

292 , different forms of Rho were isolated using gel filtration techniques in mild detergents, including

293 We show by sedimentation and gel filtration that Nck1 and CE are together in a larger

294 t Y118D alphaA-crystallin were studied using gel filtration, two-dimensional (2D) gel electrophoresis

295 ajority of which are subsequently removed by gel filtration using beads with an exclusion limit of 40

296 In addition, using gel filtration, we find that the conformational opening

297 Urine samples after gel filtration were analyzed for the presence of differe

298 -spectrometry-based platform that integrates gel filtration with activity-based protein profiling to

299 :1, 1:2, and 1:3 complexes were confirmed by gel filtration with in-line light scattering.

300 A system combining pressurized capillary gel filtration with online laser light scattering and mi