ライフサイエンスコーパス: gene disruption

1 annealing in addition to gene targeting and gene disruption.

2 sponse signaling, as being impacted by RAD6B gene disruption.

3 ng cases, supporting high rates of biallelic gene disruption.

4 ENs might be used to perform allele-specific gene disruption.

5 n the presence of TNF were enhanced by Dusp1 gene disruption.

6 g in neurons from mice with an ACCN1 (ASIC2) gene disruption.

7 gene clusters, were investigated by targeted gene disruption.

8 of mammalian cells has precluded large-scale gene disruption.

9 cal phase have been functionally analysed by gene disruption.

10 ce of cryptic chromosome rearrangements, and gene disruption.

11 was discovered using mice with targeted AQP3 gene disruption.

12 and rescue only some of the consequences of gene disruption.

13 Transposons are widely employed as tools for gene disruption.

14 DNA repair, and ocular degeneration from the gene disruption.

15 protein abundance and the fitness effect of gene disruption.

16 rhabditis elegans by chitin localization and gene disruption.

17 ing the potential for promoter activation or gene disruption.

18 T. gondii mutants deficient in TgA1 through gene disruption.

19 igenicity of swine RBC can be eliminated via gene disruption.

20 of transgenic sgRNA lines mediate efficient gene disruption.

21 ys, dramatically increasing the frequency of gene disruption.

22 me inserted into this BAC clone has no known gene disruptions.

23 d in mice homozygous for both RGS7 and RGS11 gene disruptions.

24 rculosis, we have been constructing targeted gene disruptions.

25 Ll.LtrB introns contains most viable E.coli gene disruptions.

26 targeted knockouts using suicide vectors for gene disruptions.

27 dified, or reporter genes; and even targeted gene disruptions.

28 first is the CRISPR-Cas9 system for targeted gene disruption, a largely species-agnostic method [12,

29 bodies, RNA interference or Cre-lox-mediated gene disruption abolishes corneal avascularity in mice.

30 pkc-2 gene disruption abrogated thermotaxis; a PKC-2 transgene

31 red with the unmodified crRNA and comparable gene disruption activity to the previously published sin

32 MTAN gene disruption affects the growth and pathogenicity of

33 Based on the phenotypes of the germ line gene disruptions, Akt1 has been associated with control

34 ave established CRISPR/Cas9-mediated somatic gene disruption, allowing for in vivo targeting of TSGs.

35 Pbp gene disruption also abolishes primary mitogen-induced l

36 otyping approach and observed a depletion of gene disruptions amongst high frequency deletions.

37 Gene disruption analyses revealed that deregulation of p

38 Gene disruption analysis of P. berghei PPs reveals that

39 g a yeast strain carrying an L25 chromosomal gene disruption and a plasmid-encoded FLAG-tagged L23a g

40 Via targeted gene disruption and alterations to the promoter region,

41 rct healing, we studied the effects of MCP-1 gene disruption and antibody neutralization in a closed-

42 We report the use of systematic molecular gene disruption and classical genetics for engineering f

43 Using mfa5 gene disruption and complementation studies, the authors

44 Previously, we used gene disruption and complementation to conclude that the

45 an efficient base editing window for robust gene disruption and correction.

46 icking studies relied primarily upon chronic gene disruption and dominant-negative protein expression

47 l anomalies represent natural experiments of gene disruption and dysregulation.

48 nt for future experiments involving targeted gene disruption and forward genetic screens.

49 Sequential targeted gene disruption and gene expression profiling provided e

50 lection, diverse functional effects, such as gene disruption and gene-expression changes, or associat

51 d a powerful reverse genetic tool for direct gene disruption and genome modification in vivo.

52 de A (a heptadride) was investigated through gene disruption and heterologous expression experiments.

53 o mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system.

54 support a causal relationship between KCNE2 gene disruption and lung dysfunction.

55 In addition, genetic analysis (including gene disruption and mutational studies) further strength

56 of Ald6p for phenotypes associated with ZWF1 gene disruption and on the apparent lethality resulting

57 While genome-wide gene disruption and over-expression efforts are well on

58 le for CCR5 in experimental PH, we used both gene disruption and pharmacological CCR5 inactivation in

59 elopment of biotechnological tools for plant gene disruption and repair have lagged behind the rapid

60 lpStop, a generalizable tool for conditional gene disruption and rescue in post-mitotic cells.

61 Using gene disruption and RNA interference, we provide evidenc

62 In microbiology, gene disruption and subsequent experiments often center

63 of CPe dysfunction caused by cation channel gene disruption and suggest that KCNE2 influences blood-

64 d a mechanistic connection between circadian gene disruption and the precipitation of manic episodes

65 Through gene disruption and the production of active site mutant

66 have been generated by means of IL-13Ralpha1 gene disruption and used to determine whether such defic

67 Plasmodium yoelii, we demonstrate that both gene disruptions and coding sequence insertions are effi

68 dTALENs produced high rates of site-specific gene disruptions and created strains with expected mutan

69 entional experimental perturbations, such as gene disruptions and drug treatments.

70 cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--S

71 ases the frequency of clones with homozygous gene disruptions and rescues otherwise ineffective sgRNA

72 Gene disruptions and RNA interference have now formally

73 dynamic imaging, mass spectrometry, parasite gene disruption, and chemical probes to reveal that vest

74 rtion can be used to generate a lacZ fusion, gene disruption, and epitope-tagged gene product.

75 sive replacement of intergenic space (>95%), gene disruption, and gene/gene fragment movement.

76 s indicated that purifying selection against gene disruption, and not initial integration targeting,

77 designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonho

78 This was established by targeted gene disruption applied separately to tylR and tylS toge

79 of which could not be identified by standard gene disruption approaches.

80 Existing methods for gene disruption are limited by their efficiency, time to

81 ons, but the pulmonary consequences of KCNE2 gene disruption are unknown.

82 Circadian gene disruptions are associated with the development of

83 etworks that have been defined via extensive gene disruption assays.

84 n profiles, comparative genome analysis, and gene-disruption assays should permit the determination o

85 methodology, which achieves high-throughput gene disruption at an efficiency >90% in this filamentou

86 We observed biallelic gene disruption at frequencies >1%, thus obviating the n

87 les the prediction of the effect of targeted gene disruption at the system level.

88 Through targeted gene disruption, biochemical analysis of all predicted g

89 us, both transposons are effective tools for gene disruption, but Tn7 does so with less duplication a

90 rkedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the r

91 Both gene disruption by double cross-over and gene truncation

92 Loss of PIMMS2 function through gene disruption by homologous recombination leads to nor

93 Single integrations are required for gene disruption by homologous recombination.

94 ovides a comprehensive resource for DSB-free gene disruption by iSTOP.

95 Gene disruption by the breakpoints was present in 16/31

96 RhoA gene disruption caused a strong developmental block at t

97 Conditional gene disruption causes a profound reduction in invasion

98 Gene disruption, cell biological, and immunolocalization

99 creens of strain libraries such as the yeast gene disruption collection.

100 linage (Nlrc5(moKO)) and mice without Nlrc5 gene disruption (controls).

101 Conditional gene disruption data indicate that it contributes to the

102 ults support the feasibility of using single-gene disruption data to design and construct viable geno

103 at inhibition of Panx1 using pharmacology or gene disruption delays and attenuates clinical signs of

104 Two clones with distinct Pfs230 gene disruptions (Delta1.356 and Delta2.560) and a clone

105 Regional gene disruption demonstrates that NgR1 expression is req

106 The molecular outcome of enhanced gene disruption depends upon cellular context, stimulati

107 Mice lacking K 3.1 following targeted gene disruption did not show specific pY12-K(ir)3.1 immu

108 Single-gene disruptions did not show any obvious morphological

109 ich provides a readout of gene targeting and gene disruption downstream of a targeted DNA double-stra

110 and one of them was found to be resistant to gene disruption due to the potential essential nature of

111 tiviruses to seed gain- and loss-of-function gene disruption elements, which were further deployed by

112 Ephx2 gene disruption eliminated soluble epoxide hydrolase pro

113 After spinal cord injury, PTPsigma gene disruption enhanced the ability of axons to penetra

114 s for uniform temporal control and efficient gene disruption, even in a polyclonal setting.

115 The frequency and precision of gene-disruption events observed suggest that this approa

116 We found that mice with HOXA10 gene disruption exhibited an overwhelming and fatal emer

117 Gene disruption experiments confirmed the biosynthetic g

118 Controlled gene disruption experiments indicated that the gene enco

119 Gene disruption experiments showed that three different

120 The results, further supported by gene disruption experiments, indicate that six Grl prote

121 the number of recurrent and total targets of gene disruption found in our and similar studies, we est

122 ble activity to benchmark ZFNs, with allelic gene disruption frequencies of 15-30% in human cells.

123 pending on vector dose and target cell type, gene disruption frequencies of up to 15% were achieved w

124 75 (eba-175), we achieved high (>/= 50-100%) gene disruption frequencies within the usual time frame

125 Gene disruption frequently produces no phenotype in the

126 rmation about the phenotypic consequences of gene disruption: genes that are crucial for the function

127 Using CRISPR/Cas9, we obtained efficient gene disruption (>80%) of target genes encoding proteins

128 gration into cancer-related genes leading to gene disruption has been reported.

129 lasts (MEFs) with cre/loxP-mediated vinculin gene disruption in 3D collagen I cultures.

130 Removal of BEN1 by gene disruption in a Col-0 wild-type background, on the

131 This proved highly efficient at gene disruption in a patient-derived keratinocyte cell l

132 rful new research tools that enable targeted gene disruption in a wide variety of model organisms.

133 been informed by phenotypes associated with gene disruption in animal models or by genetic linkage s

134 In a mirror image, Lnc13 gene disruption in beta-cells partially counteracts poly

135 ion, RB1 was frequently inactivated by gross gene disruption in BRCA1 hereditary breast cancer and BR

136 erved loss-of-function arises from biallelic gene disruption in class II transactivator that leaves o

137 In addition, gene disruption in mice has revealed that mouse DOT1L pl

138 These findings demonstrate that DLG4 gene disruption in mice produces a complex range of beha

139 Here we show by inducible gene disruption in mice that ADAR1 is essential for main

140 fic functions for FABP9 by means of targeted gene disruption in mice.

141 tudy investigated the consequences of Gigyf2 gene disruption in mice.

142 sive free alpha-globin caused by beta-globin gene disruption in mice.

143 nd memory paradigms was analyzed by targeted gene disruption in mice.

144 njury and to the embryonic lethality of FADD gene disruption in mice.

145 Gene disruption in mouse embryonic stem cells or zygotes

146 because of the embryonic lethality of Bcl-x gene disruption in mouse models.

147 data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months

148 In conditional plectin knockout mice with gene disruption in muscle precursor/satellite cells (Pax

149 We used targeted gene disruption in P. falciparum strain 3D7 to ablate PN

150 egrating F-actin and FA dynamics by vinculin gene disruption in primary fibroblasts.

151 Here we describe optimized strategies for gene disruption in primary myeloid cells of human and mu

152 -transposons previously used for large-scale gene disruption in Saccharomyces cerevisiae: Tn3 and Tn7

153 tranded DNA greatly stimulates Cas9-mediated gene disruption in the absence of homology-directed repa

154 HF-activated one-carbons was investigated by gene disruption in the mouse germ line.

155 Using gene disruption in the mouse, we discovered a function f

156 r this study, we have constructed a targeted gene disruption in the mshD gene that encodes mycothiol

157 ery methods in the green alga Chlamydomonas, gene disruption in the rodent malaria parasite Plasmodiu

158 site-specific nucleases now enables targeted gene disruption in the zebrafish.

159 Ns as proteins leads to efficient endogenous gene disruption in various mammalian cell types with min

160 lease proteins, providing efficient targeted gene disruption in vector-treated cell lines and primary

161 en cre allele and demonstrated Cre-dependent gene disruption in vivo.

162 provide a generally applicable protocol for gene disruption in Xenopus.

163 Gene disruption in zebrafish revealed a critical role fo

164 Arabidopsis mutants containing gene disruptions in AHA1 and AHA2, the two most highly e

165 utagenesis is an effective tool for creating gene disruptions in maize.

166 nt a relatively untapped reservoir of single-gene disruptions in neurodevelopmental disorders (NDDs).

167 ever, the current lack of tools for targeted gene disruptions in obligate intracellular microbial pat

168 We previously identified six single gene disruptions in Saccharomyces cerevisiae that allow

169 Targeted gene disruption induced by Clustered Regularly Interspac

170 Allele-specific gene disruption induced by non-homologous end-joining (N

171 Importantly, target gene disruption is complete within 2-3 days of transient

172 on of rare lung tumors, the effect of S1P(2) gene disruption is remarkably restricted to DLBCL.

173 Reduction of VDAC1 activity with targeted gene disruption is shown to diminish survival by acceler

174 mosomal complexity, whereas the frequency of gene disruption is similar in both normal and abnormal t

175 wed us to test the correlation between BtR-4 gene disruption, lack of HevCaLP, and altered Cry1A toxi

176 th 506 deletion-GFP strains, identified four gene disruptions leading to abnormal ribonucleotide-diph

177 d CLIK analysis to five screens of the yeast gene disruption library and found that it defined a sign

178 Here, we show that an Escherichia coli gene disruption library obtained using such randomly ins

179 nique to introduce plasmids into the haploid gene-disruption library to discover new interacting path

180 opoisomerase I (TOP1) into the haploid yeast gene-disruption library.

181 The provision of large populations of gene disruption lines to the research community has grea

182 al state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable elem

183 y of host DC subsets resulting from targeted gene disruption markedly inhibits the development of tra

184 l advances in developing random and targeted gene disruption methods to investigate the functions of

185 nscription machinery engineering (gTME), and gene-disruption methods such as transposon insertion or

186 Using gene disruption models of Gata1 and Nfe2, we demonstrate

187 n mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential p

188 etitive infections of C. albicans homozygous gene disruption mutants revealed an unanticipated, inhib

189 Through targeted gene disruption, mutants of Synechocystis sp. PCC 6803 w

190 erimental measurements for all viable single-gene disruption Mycoplasma genitalium strains.

191 Both dkf-1 gene disruption (null phenotype) and RNA interference-me

192 Gene disruption of AKAP150 in mice dramatically reduces

193 Finally, gene disruption of an RNA methyltransferase gene hom12 r

194 Gene disruption of c92 within STIV demonstrates that c92

195 Selective pharmacologic inhibition or gene disruption of COX-2 blocked resolution of ALI.

196 Furthermore, we found that gene disruption of Elf-1 resulted in decreased CD1D1 exp

197 Mice carrying a targeted gene disruption of IkappaBNS demonstrate dysregulation o

198 Gene disruption of IP6K2 in colorectal cancer cells sele

199 Gene disruption of IRS-2 results in failure of the beta-

200 Here we report the targeted gene disruption of PfRh2b and PfRh2a in W2mef, a parasit

201 Like disruption of syndecan-4 or caveolin, gene disruption of RhoG in mice was found to retard clos

202 hanistic basis of this phenotype by targeted gene disruption of Spnb3.

203 me sequencing of a soil isolate and targeted gene disruption of the CA4 antigen-encoding gene, we sho

204 These structural results are complemented by gene disruption of the oxygenases evdO1 and evdMO1 from

205 We carried out targeted gene disruption of the Phypa_PEX11-1 gene and compared t

206 Gene disruption of the tumor suppressor PTEN, a negative

207 Here we show that four independent gene disruptions of AHA10 result in seed coats with a tr

208 Targeted gene disruptions of all 13 predicted cellulase genes sho

209 Gene disruptions of cinA and cinQ did not lead to a sign

210 Mice with targeted gene disruptions of KATP subunits expressed in vascular

211 the unique short region MHC class I evasion genes, disruption of UL82 expression by short, interferi

212 Although CRISPR-based gene disruption offers a fast-track approach for perturb

213 As Hox gene disruption often has pleiotropic effects, the root

214 d should be useful for testing the impact of gene disruption on biofilm formation, studying the impac

215 In contrast, we find that the effect of rpd3 gene disruption on global gene expression is considerabl

216 We examined the effects of Smad3 gene disruption on infarct healing and the pathogenesis

217 This study examined the effects of nup155 gene disruption on mouse embryonic stem cells to gain in

218 paper, we studied the effect of 5LO-targeted gene disruption on the amyloid phenotype of a transgenic

219 was examined by testing the effect of dicer gene disruptions on mycovirus infection of the chestnut

220 iating genetic interactions between pairs of gene disruptions, one from each organism.

221 Many techniques, including targeted gene disruption or allele substitution, insertional muta

222 ed repair (HDR) pathways results in targeted gene disruption or gene addition, respectively.

223 ouble strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology direct

224 Nos2 gene disruption or inhibition of NOS2 activity led to an

225 Moreover, 5-LO targeted gene disruption or its in vivo selective pharmacological

226 Inhibition of iNOS by gene disruption or pharmacological inhibitors reverses o

227 Zebrafish larvae subjected to CRISPR-Cas9 gene disruption or transient suppression of dync1i2a dis

228 iNOS gene disruption or treatment with iNOS inhibitor amelior

229 ipulating genomes, including introduction of gene disruptions or corrections.

230 ght cause disease through triplosensitivity, gene disruption, or gene fusion at breakpoints.

231 dy shows that direct experimental testing of gene disruption phenotypes across species can inform com

232 We used a combination of gene-disruption platforms (Tnt1 retrotransposons, hairpi

233 EndoU gene disruption prevents AICD and normalizes c-Myc expre

234 GenVar also helps identify gene disruptions probably caused by sequencing errors.

235 The Drosophila Gene Disruption Project (GDP) has created a public colle

236 sential genes are included in the Drosophila Gene Disruption Project collection.

237 We conclude that Ankrd26 gene disruption promotes adipocyte differentiation at bo

238 IOX overexpression exacerbates, whereas MIOX gene disruption protects against, cisplatin-induced AKI.

239 ion, achieving up to 25-fold enhancements in gene disruption rates.

240 Inactivation of VSK3 by gene disruption reduces the rate of phagocytosis in cell

241 Predicting the phenotypic consequences of a gene disruption remains a significant challenge.

242 Whereas homozygous ubc13 gene disruption resulted in embryonic lethality, heteroz

243 new score can readily be used to prioritize gene disruptions resulting from any genetic variant, inc

244 global as well as endothelial-specific WNK1 gene disruption results in embryonic lethality due to an

245 The single gene disruptions revealed new genes that could not be di

246 the approximately 1 Mb interval and the only gene disruption shared between spp1 and spp3.

247 ligand (Flt3L-/-) as the result of targeted gene disruption show severe reductions in dendritic cell

248 ishes the relative virulence of clinical and gene disruption strains.

249 mptothecin mimetic, identified 190 sensitive gene-disruption strains along with rpa34.

250 To determine whether different gene disruption strategies affect the phenotype of resul

251 Standard CRISPR-mediated gene disruption strategies rely on Cas9-induced DNA doub

252 However, because different gene disruption strategies use different mechanisms to d

253 Here, we used a novel gene disruption strategy to generate the whole body dema

254 Using a gene disruption strategy, we found that Etsrp71 mutant e

255 Gene disruption studies in mice have revealed essential

256 Gene disruption studies indicated that, unlike ervA, ero

257 Gene disruption studies revealed the functional redundan

258 Gene disruption studies show that AgtA, the enzyme respo

259 Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited

260 However, current cell type-specific gene disruption techniques in flies often reduce gene ac

261 e coupled, for the first time, with targeted gene disruption techniques to measure the effect of a si

262 gate the in vivo function of Kbtbd5, we used gene disruption technology and engineered Kbtbd5 null mi

263 population genetics, the mating process, and gene disruption technology that are providing much neede

264 At least 33.9% of BCAs resulted in gene disruption that likely contributed to the developme

265 uses mouse embryonic stem cells (mESCs) with gene disruptions that can be differentiated into cardiac

266 ted a screen in Escherichia coli to identify gene disruptions that caused vesicle over- or underprodu

267 ransgene in a genome-wide screen to identify gene disruptions that enhance silencing of foreign genet

268 Here we separately inactivated by gene disruption the major sporulation-specific sigma fac

269 While F0 embryos displayed mosaic gene disruption, the phenotype appeared very penetrant i

270 y, through the study of patients with select gene disruptions, the interleukin-17 (IL-17) pathway has

271 y increased the level of stable, ZFN-induced gene disruption, thereby providing a simple technique to

272 imental models, which could include targeted gene disruption to adapt fastidious malaria parasite spe

273 ilamentous actin (F-actin) chromobodies with gene disruption to assign specific populations of actin

274 ystem that is broadly conserved and shown by gene disruption to be essential for gamete fusion.

275 protein in multicellular organisms shown by gene disruption to be essential for gamete membrane adhe

276 ressing cells in the mouse stomach; targeted gene disruption to generate mice lacking Spdef; and hist

277 KO gene disruption transformants of the U1 and U2 loci prod

278 T cells using CRISPR/Cas9-mediated targeted gene disruption under the aegis of p53siRNA in the mouse

279 ere we developed a more efficient method for gene disruption using CRISPR (clustered regularly inters

280 recombinase (VC)-mediated IEC-specific SOCS3 gene disruption (VC/HO), WT/HO littermates with floxed b

281 neal epithelial reporter gene, demonstrating gene disruption via NHEJ in vivo.

282 recombination, whereas Cas9 primarily caused gene disruption via the insertion of cotransformed DNA.

283 ar viral integration sites, including direct gene disruption, viral promoter-driven human transcripti

284 high copy number plasmids (VwkA++ cells) or gene disruption (vwkA null cells) results in an array of

285 Across tissues, clock gene disruption was associated with local inflammation b

286 After evaluating several criteria for gene disruption, we concluded that 321 of the 782 protei

287 Using targeted gene disruption, we show that human epithelial cells wit

288 ing fluorescent protein tagging and targeted gene disruption, we show that PbSR is synthesized in mac

289 Using gene disruptions, we show that PA4778, along with 7 of 1

290 These effects of Zpr1 gene disruption were confirmed and extended in studies o

291 esting that the beneficial effects of IL-1RI gene disruption were not attributable to decreased cardi

292 Chromosomal rearrangements leading to gene disruption were prevalent, affecting genes known to

293 Recently, SEMA3D gene disruptions were associated with cardiovascular def

294 Targeted gene disruptions were attempted in vivo.

295 To that end, site-specific gene disruptions were created in hvKP1 (wild type), resu

296 Gene disruptions were identified that caused differences

297 possible outcomes of genome editing include gene disruption, which has been most notably applied to

298 We demonstrate a mosaic pattern of gene disruption, which hinders the application of the te

299 uclei of human cells, thus enabling targeted gene disruption while maintaining cell viability.

300 ditor can mediate highly efficient multiplex gene disruption with minimal double-strand break inducti