ライフサイエンスコーパス: gene drive

1 crops for changing climates and agricultural gene drives).

2 writing the changes introduced by an earlier gene drive.

3 ivity, can drive reliably to fully replace a gene drive.

4 ive as well as self-propagating CRISPR-based gene drive.

5 argeting Ae. aegypti that rely on Cas9-based gene drives.

6 ls and control disease-carrying insects with gene drives.

7 ing for complex, post-normal issues, such as gene drives.

8 rovide safe biocontainment of transgenes and gene drives.

9 nets (ITNs), in addition to scenarios where gene drives act in concert with existing vector control

10 Myc oncoproteins induce genes driving aerobic glycolysis, including lactate dehy

11 ly replace a harmful wild-type allele with a gene drive allele engineered to have desired functionali

12 organisms to study how a locally introduced gene drive allele spreads to replace the wild-type allel

13 of TCR T cell immunotherapy targeting fusion gene-driven AML.

14 We examine potential barriers to Semele gene drive and suggest molecular tools that could be use

15 The sensitivities of Gene drive and Xpert for the detection of smear-microsco

16 utionary strategies to control the spread of gene drives and reverse genomic changes.

17 we discuss the different types of engineered gene drives and their potential applications, as well as

18 We provide a review of gene drives and their potential applications, as well as

19 etter molluscicides, new technologies (e.g., gene drive), and 'outside the box' strategies such as na

20 Gene editing, gene drives, and synthetic biology offer novel opportuni

21 iplinary panel discussed the complexities of gene drive applications as part of the third Sackler Col

22 al-based mathematical model to simulate each gene drive approach in a variety of sub-Saharan African

23 Within its parameter space for success, each gene drive approach provides a tool for malaria eliminat

24 Reverse genetics is a gene-driven approach that comprises specific gene target

25 Engineered gene drives are being explored as a new strategy in the

26 Gene drives are genetic elements that manipulate Mendeli

27 Gene drives are genetic modifications designed to propag

28 biotechniques, namely synthetic biology and gene drives, are discussed, and a short outlook on the f

29 ractions in C. albicans, a CRISPR-Cas9-based Gene Drive Array (GDA) was developed" incorrectly cited

30 Here, we developed a CRISPR-Cas9-based 'gene drive array' platform to facilitate efficient genet

31 ht into the behavior and potential impact of gene drives as well as the spread of insecticide resista

32 However, identification of genes driving association signals remains challenging.

33 For each targeted locus we observed a strong gene drive at the molecular level, with transmission rat

34 phage-like particles can transfer bacterial genes, driving bacterial evolution and promoting the eme

35 Engineered gene drives based on a homing mechanism could rapidly sp

36 ere we consider the potential for RNA-guided gene drives based on the CRISPR nuclease Cas9 to serve a

37 lation size and/or vectoring competency, and gene drive-based methods.

38 Gene drive-based strategies for modifying populations fa

39 ulation in Candida albicans using a modified gene-drive-based strategy that takes ~1 month to complet

40 A gene drive biases inheritance of a gene so that it incre

41 A gene drive biases the transmission of one of the two cop

42 Hence, selfish gene drive by one haplotype will impact the evolutionary

43 reatly stimulated activity of a CD4 reporter gene driven by a basic CD4 promoter and the CD4 enhancer

44 en transgenic events, containing the AmCYAN1 gene driven by a CMP promoter and the E. coli PMI gene d

45 e transient and stable expression of the p19 gene driven by a constitutive promoter as well as an eth

46 on the expression of the luciferase reporter gene driven by a constitutive ubiquitin promoter (UBI-Lu

47 t-range plasmid that contained an intact aae gene driven by a heterologous tac promoter restored the

48 we generated transgenic mice with human GCIP gene driven by a liver-specific albumin promoter.

49 xpression of PKC-theta stimulated a reporter gene driven by a mouse Bcl-xL promoter.

50 human placental alkaline phosphatase (hPAP) gene driven by a promoter containing a core ARE sequence

51 eporter line that harbors a LUCIFERASE (LUC) gene driven by a promoter that undergoes DNA methylation

52 transcription from a plasmid-based reporter gene driven by a SNAT2 genomic fragment containing the C

53 plants expressing an isopentenyltransferase gene driven by a stress- and maturation-induced promoter

54 The ATFs can rapidly induce a single gene driven by a synthetic promoter in response to intro

55 ous study in D. melanogaster used a reporter gene driven by a testis-specific promoter to show that e

56 or (PEDF) or an AAV2 vector containing a PS1 gene driven by a vascular endothelial-cadherin promoter.

57 rated in which a modified hydra beta-catenin gene driven by an actin promoter is continuously express

58 line stably expressing a luciferase reporter gene driven by antioxidant response elements.

59 here was a strong expression of the reporter gene driven by AtG3Pp4 promoter in the roots, shoots, an

60 model generated through excision of the Nf2 gene driven by Cre expression under control of a tissue-

61 driven by a CMP promoter and the E. coli PMI gene driven by either a CMP or Ubi promoter, were used t

62 Using a luciferase gene driven by either a PPAR response element or a DR-1

63 hibited IFN-induced expression of a reporter gene driven by either IFN-alpha/beta- or IFN-gamma-respo

64 A WAKL4-green fluorescent protein fusion gene driven by either the WAKL4 native promoter or the 3

65 ressed expression of the luciferase reporter gene driven by glycoprotein IX promoter in L-G cells but

66 -globin plasmids using a minimal beta-globin gene driven by hybrid promoter IHK (human ALAS2 intron 8

67 ons and express the stem cell leukemia (SCL) gene driven by its 5' enhancer.

68 hat expresses an extra copy of the mouse p53 gene driven by its endogenous promoter is utilized.

69 ty and increased transcription of a reporter gene driven by multimerized GATA4-binding DNA elements.

70 Accordingly, a reporter gene driven by the AG cis-element was able to respond to

71 and inhibited transcription from a reporter gene driven by the ASNS promoter following activation by

72 that stably expressed a luciferase reporter gene driven by the BACE1 (beta-site amyloid precursor pr

73 genic (MAFIA) mice that express an inducible gene driven by the c-fms promoter for Fas-mediated apopt

74 x8 can activate the expression of a reporter gene driven by the c-RET promoter.

75 Expression of a reporter gene driven by the CaMV 35S promoter is markedly reduced

76 boring the beta-glucuronidase (GUS) reporter gene driven by the cauliflower mosaic virus 35S (CaMV35S

77 A second construct, containing a ZFN gene driven by the constitutive CsVMV promoter and an HP

78 n of the yellow fluorescent protein reporter gene driven by the CryR1 cis-element in Arabidopsis leaf

79 A rescuing daf-9::GFP fusion gene driven by the daf-9 promoter is expressed in two he

80 With a luciferase reporter gene driven by the Dectin-1 gene promoter, the expressio

81 reporter constructs: the beta-glucuronidase gene driven by the GA-inducible Amy32b alpha-amylase pro

82 asmid-borne chloramphenicol-resistance (cat) gene driven by the lac promoter.

83 ing the urokinase-type plasminogen activator gene driven by the major urinary protein promoter.

84 mbinatorial codes with a luciferase reporter gene driven by the myelin basic protein promoter define

85 resulting in the expression of the cyclin D1 gene driven by the powerful enhancer of the immunoglobul

86 ltered expression of the luciferase reporter gene driven by the promoter from the heat-inducible gene

87 Using a reporter gene driven by the R2 regulatory element, we analyzed th

88 ne plasmid and a beta-galactosidase reporter gene driven by the sigma32-dependent groE promoter on an

89 imulated expression of a luciferase reporter gene driven by the Stat3 promoter.

90 , CJ2-gD2, that contains 2 copies of the gD2 gene driven by the tetracycline operator (tetO)-bearing

91 constructed to express a firefly luciferase gene driven by the viral M3 promoter (M3FL).

92 ed by a more robust activation of a reporter gene driven by unfolded protein response activation upon

93 amplicon-6 vectors containing a GFP reporter gene driven by WT viral promoter or by promoter mutated

94 e introduced a construct carrying both a ZFN gene, driven by a heat-shock promoter, and its target in

95 t transgenic mice expressing the human B-myb gene, driven by the basal cytomegalovirus promoter, comp

96 eening purposes when the expression of a GFP gene, driven by the reactivation of endogenous stem cell

97 rpes simplex virus thymidine kinase reporter genes driven by a constitutive ubiquitin promoter into a

98 E (apoE)(-/-)/transgenic mice carrying LacZ genes driven by an endothelial TIE2 promoter.

99 Ideally, expression of therapeutic genes driven by cancer-specific promoters would only tar

100 of baculoviruses carrying viral or cellular genes driven by CMV immediate-early promoter.

101 intained modest activation of cleavage-stage genes driven by conventional promoters but did not activ

102 sp. red fluorescent protein (DsRed) reporter genes driven by either CaMV35S or intron-interrupted mai

103 An NF-kappaB-dependent subset of genes driven by oncogenic PI3K mutations was also identi

104 orescent protein and red fluorescent protein genes driven by promoters that are functional in maize (

105 H3 displacement differed between genes driven by promoters with 'open'/DPN and 'closed'/O

106 rexpression of TEF had no effect on reporter genes driven by SM22alpha, smooth muscle alpha-actin, or

107 of different spliced variants produced from genes driven by steroid hormone-dependent promoters.

108 vious work shows that expression of reporter genes driven by testis-specific promoters is considerabl

109 GLK genes driven by the Arabidopsis epidermal FIDDLEHEAD (FD

110 g luciferase and beta-galactosidase reporter genes driven by the Col1a2 enhancer/promoter and the CTG

111 opment, and can be visualized using reporter genes driven by the GLABRA (GL)2 promoter as markers.

112 lyse experimentally stochastic expression of genes driven by the Synechococcus elongatus circadian cl

113 The expression of exogenous genes driven by the TBG promoter demonstrates to be much

114 Genes driven by these repeat-containing promoters show s

115 actions among transcription factors, the gap genes, driven by maternal inputs.

116 ntranslated regions of known disease-causing genes, driven by SOD1, TARDBP, FUS, VCP, OPTN and UBQLN2

117 The MYB80 homolog genes, driven by the AtMYB80 or their respective promote

118 Induction of reporter genes, driven by truncated Ifit1 promoters, identified t

119 tudy the impact of polyandry on a well-known gene drive, called t haplotype, in an intensively monito

120 plication, our results indicate that a viral gene drive can be used as a strategy to suppress a viral

121 udies have demonstrated the potency by which gene drives can operate within insects and other organis

122 CRISPR-based gene drives can spread through wild populations by biasi

123 We sought compounds that inhibited genes driving cancer but not normal growth and hypothesi

124 amplifications and codeletions and delineate genes driving cancer metabolism from those that are neut

125 Dependency Map, the latter of which catalogs genes driving cancer progression.

126 RNA-guided gene drives capable of spreading genomic alterations mad

127 e inserted at the same genomic location as a gene drive, carrying two gRNAs that cut on either side o

128 focused on the mechanisms by which Rb family genes drive cell cycle exit following DNA damage inducti

129 observed in human cancers, proposes that the genes driving cell proliferation induce DNA replication

130 tion was associated with the upregulation of genes driving ceramide biosynthesis, an event dependent

131 morphisms within target sites for Cas9-based gene drive (CGD) and that these "drive resistant alleles

132 ension of sensory experience based solely on gene-driven changes in receptor organization.

133 tor genes, a marker gene, and the autonomous gene-drive components are introgressed into approximatel

134 of regulatory sequence from the murine IL-2 gene drives consistent expression of a green fluorescent

135 ver, an accidental or premature release of a gene drive construct to the natural environment could da

136 on, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each g

137 lar and modeling data suggest that a Y-CHOPE gene drive could be a viable tool for vertebrate pest co

138 For example, RNA-guided gene drives could potentially prevent the spread of dise

139 xistence of many yet-to-be discovered cancer genes driving CRC development, as well as other human ca

140 tical work has suggested the use of modified gene drive designs that are limited in spread such as da

141 ating gene drive technologies, highlight new gene drive designs that might achieve better outcomes, a

142 r sex bias that could be incorporated within gene-drive designs.

143 Variants in ribosomal protein (RP) genes drive Diamond-Blackfan anemia (DBA), a bone marrow

144 pecific genes and repression of pluripotency genes drives differentiation of embryonic stem cells (ES

145 albicans haploids, each carrying a different gene drive disabling a gene of interest, we are able to

146 troduced functional descriptions of how each gene drives disease, summarized into the 10 cancer Hallm

147 somes to collapse a mosquito population, and gene drive disrupting a fertility gene and thereby achie

148 rest in environmental releases of engineered gene drives due to recent proof of principle experiments

149 ion: 'selected expression regulators' (SERs)-genes driving dysregulated transcriptional programs in c

150 Here, we study gene drive effectiveness, fitness, and inducible drive s

151 ese elements use Cas9 produced in trans by a gene drive either to inactivate the cas9 transgene (e-CH

152 Using a luciferase gene driven either by a PPAR response element or by a DR

153 to spread in wild populations, CRISPR-based gene-drive elements would provide new ways to address ec

154 discuss growing evidence that implicates AD gene-driven ELN disruptions as not only the antecedent p

155 node controlling translational activation of genes driving EMT and ultimately tumour progression.

156 gene (e-CHACRs) or to delete and replace the gene drive (ERACRs).

157 ensus (CGC) describes a curated catalogue of genes driving every form of human cancer.

158 investigating the rates and patterns of new gene-driven evolution of GGI networks in the human and m

159 ives as flexible safeguarding strategies for gene drive experiments by showing that their performance

160 CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome

161 should enable the development of safe CRISPR gene drives for diverse organisms.

162 icies regarding the safety and regulation of gene drives for the manipulation of wild populations.

163 , here we develop several CRISPR-based split gene drives for use in this vector.

164 olid tumors for specific silencing of target genes driving growth and/or metastasis.

165 The use of gene drives has sparked significant enthusiasm for genet

166 netic mechanisms for limiting or eliminating gene drives have been proposed and/or developed, includi

167 Gene drives have garnered much attention for their poten

168 CRISPR-based homing gene drives have sparked both enthusiasm and deep concer

169 Gene drives have the potential to rapidly replace a harm

170 ers a proof of principle for the design of a gene drive in viruses.

171 nvestigate gene flow, and the propagation of gene drives in Anopheline populations.

172 By delineating expression outlier genes driven in cis by CNAs, we identified putative canc

173 As is dynamically induced at most of the key genes driving innate immunity and inflammation.

174 nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the dou

175 This, coordinated with induction of genes driving intestinal differentiation, may be require

176 e first direct evidence that the spread of a gene drive is hampered by reproductive behaviour in a na

177 We argue that "balanced gene drive" is a sufficient explanation for the trend th

178 Controversy exists as to whether viral genes drive it directly or less directly orchestrate the

179 ified an enhancer ~5 kb upstream of the IFNK gene driving its expression in keratinocytes.

180 identified an enhancer upstream of the Wnt9a gene driving joint-specific expression in transgenic rep

181 We predicted genes driving lineage divergence and explored their expr

182 Our predicted genes driving lineage divergence provide good starting p

183 strongly point to FAT1 as a tumor suppressor gene driving loss of chromosome 4q35, a prevalent region

184 idered a symbol of maleness, as it encodes a gene driving male sex determination, Sry, as well as a b

185 all cage trials show that single releases of gene-drive males robustly result in efficient population

186 10 generations following a single release of gene-drive males.

187 We also identified six genes driving malignant tumor progression and a new huma

188 Gene drives may be capable of addressing ecological prob

189 Here we propose a novel gene drive mechanism that could be engineered using a co

190 method of disease prevention but requires a gene drive mechanism to spread these traits to high freq

191 CI is a gene-drive mechanism impacting population structure(6) a

192 ly to be lost from the environment; however, gene drive mechanisms enhance the invasiveness of introd

193 A wide range of gene drive mechanisms have been proposed that are predic

194 o analyze the loss probabilities for several gene drive mechanisms, including homing endonuclease gen

195 The mutated PDE3A gene drives mechanisms that increase peripheral vascular

196 ools to track and assess the impact of multi-gene driven metabolic resistance to pyrethroids, helping

197 pment of CRISPR-Cas9 reagents as a source of gene drive, more advanced technologies at driving malene

198 Gene drive mosquitoes constitute a promising set of tool

199 low copy-number gain of one or more of these genes drives neoplasia is not known.

200 NST progression useful for identifying novel genes driving neurofibroma and MPNST pathogenesis.

201 re locally fixed alleles as the target for a gene drive on a particular island.

202 s that aid in the perpetuation of MLL fusion gene driven oncogenic programs are being defined, presen

203 To identify the genes driving osteosarcoma development and metastasis, w

204 rize below some key insights from the recent gene-driven phase of research on Werner syndrome, a heri

205 ew analyses are needed as existing models of gene drive primarily focus on nonseasonal or nonspatial

206 We find that programmed modification during gene drive propagation could serve as a potent safeguard

207 However, CRISPR-based homing gene drives proposed for this purpose have faced a major

208 llowed by proper regulatory integration of a gene drive rapid evolution of bacterial pathogens.

209 However, single gene-driven recombinase lines mark relatively broad and

210 tion; inappropriate expression of HIF target genes drives renal carcinogenesis.

211 aborators, we show that socially responsible gene drives require 0.5 < s < 0.697, a rather narrow ran

212 Here, we develop a recoded gene-drive rescue system for population modification of

213 in the cytosolic 5' nucleotidase II (NT5C2) gene drive resistance to thiopurine chemotherapy in rela

214 of genomic tools and products (e.g., CRISPR, gene drives, RNAi, synthetic biology, and genetically mo

215 We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles

216 ose from the Xenopus laevis mespb (Xl-mespb) gene drive segmental expression in transgenic zebrafish.

217 We describe the successful transmission of a gene drive sequence between distinct strains of human cy

218 that includes transgenic females results in gene drive since females carrying the allele are favored

219 These results highlight how the individual genes driving speciation can be embedded within an activ

220 A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was developed to deliver a

221 the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the ut

222 Current gene drive strategies rely on sexual reproduction and ar

223 nts for successful deployment of a HEG-based gene drive strategy can be satisfied in a model dipteran

224 Here, we show that any transposon-mediated gene drive strategy must have an exceptionally low rate

225 We introduce a novel gene-drive strategy termed Y-CHromosome deletion using O

226 ty in vector population dynamics facilitates gene drive success compared with nonseasonal analyses.

227 If gene drives successfully eradicated these invasive popul

228 ypes or to what extent the same or different genes drive such convergence.

229 ependent rearrangement of endogenous TCRbeta genes, driving surface expression of novel TCRs.

230 A CRISPR-based gene drive system can allow for the forced propagation o

231 Here, we report on a gene drive system that allows the spread of an engineere

232 creation of a synthetic threshold-dependent gene drive system, designated maternal-effect lethal und

233 sequences show promise as part of a TE-based gene drive system.

234 Here, we develop an analogous CRISPR-based gene-drive system for the bacterium Escherichia coli tha

235 CRISPR)-associated protein 9 (Cas9)-mediated gene-drive system in the Asian malaria vector Anopheles

236 ctor control strategies utilizing engineered gene drive systems are being developed as a means of rep

237 organisms also suggests that reprogrammable gene drive systems based on these nucleases may be capab

238 ant mosquito strains make urgent research of gene drive systems capable of moving effector genes into

239 een invasiveness and containment for the six gene drive systems currently being considered for the co

240 Highly efficient gene drive systems have recently been developed in insec

241 as9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector

242 We report the efficacy of CRISPR-Cas9 gene drive systems in wild and laboratory strains of the

243 CRISPR-Cas9-based gene drive systems possess the inherent capacity to spre

244 Gene drive systems that enable super-Mendelian inheritan

245 ic approach to developing safe and effective gene drive systems that includes defining the requiremen

246 Consequently, there is also interest in gene drive systems that, while strong enough to bring ab

247 Interest in developing gene drive systems to control invasive species is growin

248 simplify the evaluation of novel Cas9-based gene drive systems.

249 e the confinement properties of a variety of gene drive systems.

250 ting human malaria parasites and to generate gene-drive systems capable of introgressing the genes th

251 Gene-drive systems developed in several organisms result

252 Cas9/gRNA-mediated gene-drive systems have advanced development of genetic

253 Gene-drive systems in diploid organisms bias the inherit

254 he complementary mouse genetic approaches of gene-driven, targeted mutagenesis and phenotype-driven,

255 P007280, meets the minimum requirement for a gene drive targeting female reproduction in an insect po

256 squito populations than previously developed gene drives targeting female fertility.

257 Gene drive technologies designed to eliminate a populati

258 accidental spread posed by self-propagating gene drive technologies, highlight new gene drive design

259 s potential advantages for improving current gene-drive technologies for field population modificatio

260 other emerging techniques, such as advancing gene-drive technologies, are summarized, as well as curr

261 alleles to form a trans-complementing split-gene-drive (tGD) and demonstrate its ability to promote

262 Methods such as gene drives that aim to genetically modify large mosquit

263 expedite the development of effector-linked gene drives that could safely control wild populations o

264 Self-replicating gene drives that modify sex ratios or infer a fitness co

265 ng alleles that confer phenotypic traits via gene drives that result in reduced transmission are expl

266 demonstrate that enhancers within bystander genes drive the expression of genes such as Otx and Isle

267 Mutated protein-coding genes drive the molecular pathogenesis of many diseases,

268 with the promoters of both the Myf5 and MyoD genes drives the de novo myogenesis in satellite cells e

269 the rapid induction of these circadian clock genes drives the resetting process.

270 in the intergenic regions of salt-responsive genes drives the RNAP redistribution for reprograming th

271 analyses designed to highlight the specific genes driving the aggregate signal, we confirmed associa

272 and the activation of increasing numbers of genes driving the chosen developmental direction.

273 e also developed a procedure for identifying genes driving the concordance of the genomics profiles a

274 ind a significant delay in the expression of genes driving the generation only of later- but not earl

275 er, the role of genomic changes in metabolic genes driving the tumour metabolic shift remains to be e

276 tem genetics approach identified lung tissue genes driving the variation in lung function and suscept

277 Thirty-three of 73 genes drove the pathway annotation that included extrace

278 tion, in addition to those in protein-coding genes, drove the evolution of uniquely human biological

279 Engineered gene drives - the process of stimulating the biased inhe

280 p2c to triply occupied sites at TSC-specific genes, driving their expression.

281 To identify the genes driving this extensive phenotypic variation, we pe

282 to create, streamline and improve synthetic gene drives, this is rapidly changing.

283 ing two gRNAs that cut on either side of the gene drive to excise it.

284 s a threshold condition for the ability of a gene drive to invade a partially resistant population.

285 ential approaches to disease control utilize gene drive to spread anti-pathogen genes into the mosqui

286 nuclease-based homing reaction as a form of gene drive to spread those genes through target populati

287 articipant, and compared the accuracy of the Gene drive to that of the Xpert MTB/RIF assay using M. t

288 Deciding to use gene drives to control and suppress pests will involve m

289 e findings could expedite the development of gene drives to suppress mosquito populations to levels t

290 the additional loss of one copy of the Fbxw7 gene drives tumor development in a range of epithelial t

291 s, leading to the widely held view that this gene drives tumor progression, but this hypothesis has n

292 ut may be improved by better knowledge about genes driving tumor aggressiveness.

293 of the pushed wave can be stopped by making gene drives uniquely vulnerable ("sensitizing drive") in

294 tion found in bird beaks to investigate what genes drive vertebrate facial morphogenesis.

295 e molecular mechanisms by which the 1918 PB2 gene drives viral pathogenicity.

296 alovirus (human herpesvirus 5) and show that gene drive viruses can efficiently target and replace wi

297 sgene's fitness cost and the efficacy of the gene drive will be more crucial than any evolutionary ad

298 ushed wave" regime, the spatial spreading of gene drives will be initiated only when the initial freq

299 A GUS-reporter gene driven with the putative LeMAN5 promoter (-543 to +

300 ities for the tuberculosis case detection of Gene drive, Xpert, and smear microscopy were 45.4% (95%