ライフサイエンスコーパス: gene editing

1 udes a diverse array of molecules useful for gene editing.

2 ions were generated via CRISPR/Cas9-mediated gene editing.

3 ort palindromic repeats-associated protein 9 gene editing.

4 subjected to shRNA knockdown or CRISPR-Cas9 gene editing.

5 cations for disease modeling and therapeutic gene editing.

6 for more efficient plant transformation and gene editing.

7 rthog RELA orthologue have been generated by gene editing.

8 and post-transcriptional control of in vivo gene editing.

9 tations in Arabidopsis At2OGO by CRISPR/Cas9 gene editing.

10 ase models and holds promise for therapeutic gene editing.

11 d promises to overcome a bottleneck in plant gene editing.

12 we labeled endogenous RAF1 with mVenus using gene editing.

13 determining both efficacy and specificity of gene editing.

14 s-associated protein 9 endonuclease-mediated gene editing.

15 biallelically engineered cells after CRISPR gene editing.

16 d Fas that were engineered using CRISPR/Cas9 gene editing.

17 EP290 mice showed rapid and sustained CEP290 gene editing.

18 g the first clinical targets for therapeutic gene editing.

19 y be utilized to produce KO/KI marmosets via gene editing.

20 ront of biotechnology, synthetic biology and gene editing.

21 e precise control of genome function without gene editing.

22 "write-protected" cells that prevent future gene editing.

23 P complexes for in vitro and in vivo somatic gene editing.

24 s exhibited normal expression patterns after gene editing.

25 rgeting including checkpoint inhibitors, and gene editing.

26 selection, RNA interference and potentially gene editing.

27 A inhibition, gene activation and programmed gene editing.

28 cartilage or to generate disease models via gene editing.

29 candidates for alternate approaches such as gene editing.

30 By integrating CRISPR-mediated gene editing, activation and repression technologies to

31 ne tuning of Cas9 expression to achieve high gene editing activity without detectable toxicity.

32 l models to further explore the potential of gene editing against FA, with the eventual aim to obtain

33 d short palindromic repeat (CRISPR)-mediated gene editing and acetate supplementation of the culture

34 s9 nickase activity resulted in mono-allelic gene editing and avoidance of undesired mutagenesis.

35 Here, we have combined CRISPR gene editing and engineered separation-of-function mutan

36 unteer cells with variant haplotypes, CRISPR gene editing and functional reconstitution.

37 o be applied to improve the precision in the gene editing and immunotherapy in the future.

38 CRISPR/Cas9 gene editing and mass spectrometry-based methods reveale

39 CRISPR-Cas gene editing and messenger RNA-based protein replacement

40 protocol should broaden applicability of HSC gene editing and pave its way to clinical translation.

41 s12a toolkit for efficient and rapid genomic gene editing and regulation in fission yeast.

42 oblasts and a combination of CRISPR-mediated gene editing and RNAi-mediated gene silencing in human c

43 We used gene editing and somatic cell nuclear transfer to engine

44 In this context, CRISPR-based gene editing and targeting technologies have proved trem

45 he UMOD gene was generated using CRISPR-Cas9 gene editing and the effect of this mutation on mononucl

46 We also discuss the importance of NMD for gene editing and tumor evolution, and how inhibiting NMD

47 Here, using single-molecule analysis, gene editing and zebrafish live-cell imaging, we report

48 ablate Axl on tumor cells using CRISPR/Cas9 gene editing, and by targeting Mertk in the tumor microe

49 r the development of novel gene replacement, gene editing, and cell replacement therapies for cone dy

50 mprovement through diagnostic marker design, gene editing, and gene stacking technologies.

51 tissue engineering, including gene delivery, gene editing, and subpopulation isolation.

52 fundamental immunology, genetic engineering, gene editing, and synthetic biology exponentially expand

53 Using CRISPR/Cas9 gene editing, antibody staining, and electron microscopy

54 Beyond the gene editing applications enabled by the nuclease CRISPR

55 site discrimination must be demonstrated for gene editing applications.

56 itors as potential mechanisms of control for gene editing applications.

57 s and primary cells for in vitro and in vivo gene editing applications.

58 this system has been widely used for various gene editing applications.

59 We propose that this one step gene editing approach can be used to improve the specifi

60 ins respond to biomechanical cues, we used a gene editing approach to determine if a specific region

61 Here, we used primarily a CRISPR/Cas9 gene-editing approach to characterize the roles of a key

62 We show here, using a gene-editing approach, that a point mutation that elimin

63 ted RNPs, could not be reached with a single gene-editing approach.

64 This protocol thus expands CRISPR-based gene editing approaches beyond models of robust T cell a

65 Future trials should use superior gene editing approaches to improve therapeutic efficacy.

66 /CRISPR-associated protein 9 (Cas9)-mediated gene editing approaches, we establish that PAR4-dependen

67 to the generation of off-target mutations by gene editing approaches, we test the specificity of TALE

68 Using a combination of gene-editing approaches, quantitative imaging, and bioch

69 control growth and development; and how new gene-editing approaches, the redesign of plant cell wall

70 pathways altered in PD can be reversed using gene-editing approaches.

71 Advances in gene editing are leading to new medical interventions wh

72 concept studies establish the feasibility of gene editing as a therapeutic approach for cancer and va

73 multiplexing, lipoMSN maintained significant gene-editing at each gene target despite reduced dosage

74 We propose a simple, robust yet safe gene-editing-based therapy for IPEX and IPEX-related dis

75 Here, we used gene editing by CRISPR-Cas9 to knock out the oncogene p5

76 Gene editing by CRISPR/Cas9 is commonly used to generate

77 low at 18 degrees C, enabling modulation of gene editing by temperature.

78 Moreover, in trans paired nicking gene editing can efficiently and precisely add large DNA

79 aced short palindromic repeats (CRISPR)/Cas9 gene editing can knock down pro-degenerative genes in RG

80 Gene editing can potentially overcome this issue by perm

81 Gene editing can rapidly improve a range of crop traits,

82 etion generated through CRISPR/Cas9-mediated gene editing caused perinatal lethality and SHH-related

83 biomolecules are also delivered to cells for gene editing, cell reprograming, therapy, and other purp

84 124 knockout cells generated by CRISPR-based gene editing compared scramble control.

85 at one-step zygotic injections of CRISPR/Cas gene editing complexes provide a quick and powerful tool

86 Gene editing confirmed that this mutation can drive arte

87 adeno-associated virus 9 (AAV9)-CRISPR/Cas9 gene editing construct designed for eliminating proviral

88 ology is now routinely applied for efficient gene editing, contributing to advances in biomedical sci

89 Our experiments revealed that gene editing could effectively restore Fancf function vi

90 ing new models, single-cell technologies and gene editing, could provide new insights into the pathog

91 Using CRISPR/Cas9-mediated gene editing, coupled with endocrine cell differentiatio

92 CRISPR gene editing creates indels in targeted genes that are d

93 , inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer

94 The present CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) delivery

95 Although templated gene editing efficiencies were low (<=6%), FA corrected

96 However, the gene editing efficiency in citrus via CRISPR technology

97 ng a single gene, the lipoMSN achieved a 54% gene-editing efficiency, besting the state-of-art Lipofe

98 d cell-type-specific delivery of CRISPR/Cas9 gene editing elements remains a challenging open problem

99 ne-identified PD patients and that following gene-editing, EMVs reflect a corrected state.

100 sCas12a improves the efficiency of multiplex gene editing, endogenous gene activation and C-to-T base

101 8(+) T cells with Pdia3, Mgat5, Emp1 or Lag3 gene editing enhances the survival of GBM-bearing mice i

102 ver, it is not facile to forensically detect gene-editing events at the molecular level, as no foreig

103 Cas12a gene editing expands the genome-engineering toolbox in D

104 s BdFTL1 and BdFTL2 using overexpression and gene-editing experiments.

105 intrinsic FA DNA repair defects may obstruct gene editing feasibility.

106 tes of AAV targeting, and better predict the gene editing footprint after AAV-CRISPR delivery.

107 CRISPR-Cas9 has led to great advances in gene editing for a broad spectrum of applications.

108 and demonstrating the feasibility of CRISPR gene editing for cancer immunotherapy.

109 al of iterative, multiplexed CRISPR-mediated gene editing for functional analysis of multigene cluste

110 showcase the novel application of programmed gene editing for functional genomics in schistosomes.

111 ration of mouse models of hCFTR mutations by gene editing for in vivo testing of new CF therapies.

112 h may therefore prove beneficial for ex vivo gene editing, for enhanced platelet production, and for

113 and identify a promising candidate for human gene editing from Bacillus hisashii, BhCas12b.

114 Gene editing, gene drives, and synthetic biology offer n

115 The clinical application of CRISPR-Cas9 gene editing has been eagerly awaited since the first de

116 ular model organism Drosophila melanogaster, gene editing has so far relied exclusively on the protot

117 CRISPR-Cas9 gene editing has transformed our ability to rapidly inte

118 n the functional consequences of CRISPR-Cas9 gene-editing has not yet been assessed.

119 Recent advances in gene editing have been enabled by programmable nucleases

120 ediated gene replacement, gene silencing and gene editing have helped AAV gain popularity as the idea

121 utants generated by the CRISPR/Cas9-mediated gene editing have increased FLS2 accumulation and enhanc

122 dea, recent advances, including CRISPR-based gene editing, have made possible systematic screens for

123 biquitination, generated through CRISPR/Cas9 gene editing, here, we show that this modification promo

124 For FA patients, gene editing holds promise for therapeutic applications

125 Proteomic analysis and CRISPR/Cas9 gene editing identified the inflammatory glycoprotein PT

126 Combined with new advances in gene editing, imaging, and genomic analysis, brain organ

127 quencing, transgenic technology, CRISPR-Cas9 gene editing, immunoblotting, and fluorescence resonance

128 r64Cys in CDC42 through CRISPR/Cas9-mediated gene editing in a Caenorhabditis elegans model.

129 is sufficient to mediate tightly controlled gene editing in a variety of tissues, allowing detailed

130 Disruption of EMP3 by CRISPR/Cas9 gene editing in an immortalised human erythroid cell lin

131 In addition, CRISPR-mediated gene editing in Bombyx mori establishes an essential fun

132 upporting both the feasibility and safety of gene editing in cell therapy.

133 nology has revolutionized rapid and reliable gene editing in cells.

134 we describe a platform for efficient Cas12a gene editing in Drosophila We show that Cas12a from Lach

135 R-Cas technologies have enabled programmable gene editing in eukaryotes and prokaryotes.

136 Targeted gene editing in hematopoietic stem cells (HSCs) is a pro

137 echnology is a useful tool to induce precise gene editing in HLA genes to enable the characterization

138 Depletion of A20 using gene editing in human macrophage-like cells (THP-1) sign

139 the CRISPR/Cas system has been utilized for gene editing in many plant species, including important

140 asses all hitherto existing alternatives for gene editing in many ways, it is one of the most frequen

141 his study, we show that CRISPR/Cas9-mediated gene editing in memory CD8 T cells precludes their proli

142 Here, we use gene editing in mouse C2C12 myoblasts and show that ZBED

143 TORC1 signaling was confirmed by CRISPR-Cas9 gene editing in normal and IPF fibroblasts, as well as i

144 In vivo gene editing in post-mitotic neurons of the adult brain

145 single guide RNA cassette enables efficient gene editing in primary murine T cells as well as a scre

146 urthermore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a subst

147 aced short palindromic repeats (CRISPR)/Cas9 gene editing in RGCs in vivo To our knowledge, this is t

148 arious tissue origins without any detectable gene editing in the absence of doxycycline.

149 resistance conferred by precise CRISPR/Cas9 gene editing in the chicken.

150 lexed CRISPR-based approach for simultaneous gene editing in the complex seven-member human airway tr

151 Furthermore, NCs produce robust gene editing in vivo in murine retinal pigment epitheliu

152 chnology for memory CD8 T cells to undertake gene editing in vivo, for the first time, to our knowled

153 ot Acidaminococcus spec., can mediate robust gene editing in vivo.

154 with implications for the use of CRISPR-Cas9 gene-editing in cancer cells.

155 s efficient DNA cleavage in vitro as well as gene-editing in cells with no unexpected off-target effe

156 fective CRISPR delivery system for multiplex gene-editing in the liver.

157 ntroducing pfk13 C580Y or R539T mutations by gene editing into local parasites conferred high levels

158 Plant gene editing is typically performed by delivering reagen

159 CRISPR/Cas9 guided gene-editing is a potential therapeutic tool, however ap

160 man cells that loss of RAZUL by CRISPR-based gene editing leads to loss of E6AP at proteasomes.

161 throughput technologies, such as CRISPR/Cas9 gene editing, liquid chromatography-tandem mass spectrom

162 ional hotspot, after delivery of CRISPR/Cas9 gene editing machinery with adeno-associated virus.

163 emerged as a preferred method for delivering gene-editing machinery to target cells, but a major chal

164 acquisition, off-target potential of CRISPR gene editing/manipulation, and tool development using ho

165 Using CRISPR/Cas9 gene-editing, MARCH6 overexpression, and immunoblotting,

166 ty of Ku-DBi's was also revealed in a CRISPR gene-editing model where we demonstrate that the efficie

167 lated with modified cholesterols can deliver gene-editing mRNA to the liver microenvironment at clini

168 therapeutic applications such as CRISPR-Cas9 gene editing, mRNA vaccination, and other mRNA-based reg

169 therapeutics and the improvement of precise gene editing now pave the way to applications such as ca

170 Gene editing nuclease represented by Cas9 efficiently ge

171 escent reporters, protein nanocages, and the gene-editing nuclease Cas9, with up to 5-fold higher exp

172 Gene editing of a small deletion in the first coding exo

173 etastasis and invasion in the muscle through gene editing of a specific alpha6 integrin extracellular

174 The results suggest that gene editing of a specific alpha6 integrin extracellular

175 CRISPR/Cas9 gene editing of HLA-B*38:01:01:01 homozygous EBV B cell

176 We evaluate gene editing of HSV in a well-established mouse model, u

177 ut not CRISPR/Cas9, mediate highly efficient gene editing of HSV, eliminating over 90% of latent viru

178 Congruently, gene editing of LAYN in human CD8+ T cells reduced direc

179 Precise gene editing of mitochondrial DNA (mtDNA) is essential f

180 Gene editing of mouse Ldlrad3 or human LDLRAD3 results i

181 Here, we showed that the CRISPR-Cas9 gene editing of TaGW7, a homolog of rice OsGW7 encoding

182 ever, few approaches have been developed for gene editing of these cell types, likely owing to their

183 The effects of gene editing on grain morphology and weight traits were

184 ugh, unbiased investigation of the impact of gene editing on hematopoietic stem and progenitor functi

185 o determine the functional consequences OTX2 gene editing on the population of cells derived from OTX

186 entiate whether a mutation has resulted from gene editing or from traditional breeding techniques; (i

187 C) is able to accurately predict CRISPR/Cas9 gene editing outcomes in early vertebrate embryos.

188 proaches can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopus lae

189 tand this dichotomy, we established a robust gene editing platform to rapidly address the consequence

190 al of targeted gene correction using various gene editing platforms.

191 o major genotoxicity was associated with the gene editing process, paving the way for an alternative,

192 stimuli is not significantly impacted by the gene editing process.

193 research has led to diverse applications in gene editing, programmable transcriptional control, and

194 CRISPR-Cas9 gene editing provides a powerful tool to enhance the nat

195 anded oligodeoxynucleotides mediated precise gene editing rates, and effectively reduces on-target in

196 biological samples is critical for assessing gene-editing reagent delivery efficiency, retention, per

197 Developmental regulators and gene-editing reagents are delivered to somatic cells of

198 ly outpacing detection and quantification of gene-editing reagents.

199 fusion motif may find broad applications in gene editing research and therapeutics.

200 usal locus through mapping-by-sequencing and gene editing, respectively, and performed metabolic, cel

201 Gene editing resulted in rapid depletion of Ov-GRN-1 tra

202 However, CRISPR-mediated gene editing revealed that PKA and AMPK are not required

203 s and enhances delivery of nucleic acids and gene editing ribonucleoproteins (RNPs) formulated with b

204 or each to be targeted using allele-specific gene-editing, RNAi, or small-molecule approaches.

205 ght be especially suited to gene therapy and gene editing settings in which preservation of immunity

206 ese insights, advances in protein design and gene editing should pave the way for breakthroughs towar

207 CRISPR-mediated gene editing shows promise to cure genetic pathologies,

208 (FAF1) in DLD-1 CRC cells using CRISPR/Cas9 gene editing; some cells were transfected with plasmids

209 Recent developments in gene editing, speed breeding and genome assembly techniq

210 However, testing gene editing strategies for on-target efficiency and off

211 Current CRISPR-mediated gene editing strategies in T cells require in vitro stim

212 o highlight future opportunities in emerging gene editing strategies that may offer advantages for tr

213 e nucleotide substitutions(1,2), we explored gene editing strategies to disrupt dominant mutations ef

214 l disease-modifying drugs, gene addition and gene editing strategies, matched sibling donor haematopo

215 CRISPR-Cas9 gene-editing strategies have revolutionized our ability

216 gene therapy and contrast gene therapy with gene-editing strategies.

217 We anticipate this dataset and gene editing strategy to enable functional genetic studi

218 However, our CRISPR-Cas9 gene editing studies indicate that specific mutations in

219 RGR, a Streptococcus pyogenes (Sp)Cas9-based gene editing system for Plasmodium that utilizes a riboz

220 larly interspaced short palindromic repeats) gene editing system often require viral vectors, which p

221 The CRISPR guide RNA is essential for gene editing systems.

222 e CRISPR/Cas9 system has been a democratized gene editing technique and widely used to investigate ge

223 CRISPR-Cas9 is a powerful gene editing technique that can induce mutations in a ta

224 The CRISPR-Cas9 system is a gene editing technique that has been used to specificall

225 Developments in gene editing techniques provide powerful tools for overc

226 -specific genome modification of HSPCs using gene editing techniques such as CRISPR-Cas9 has shown gr

227 SORT is compatible with multiple gene editing techniques, including mRNA, Cas9 mRNA/singl

228 Gene-editing techniques are currently revolutionizing bi

229 , members of which have found application in gene-editing techniques(1,2).

230 of characterizing new varieties generated by gene-editing techniques.

231 Moreover, gene therapy and gene editing technologies are being used to create stem

232 Gene editing technologies have enabled production of tar

233 Implementation of gene editing technologies such as CRISPR/Cas9 in the man

234 genome sequencing along with development of gene editing technologies.

235 luripotent stem cells, CRISPR-Cas9 and other gene-editing technologies, the race to create 'off-the-s

236 tomato mutants (Deltawak1) using CRISPR/Cas9 gene editing technology and investigated the role of SlW

237 Using CRISPR-Cas9 gene editing technology and rescue assays, we functional

238 Recent advances in CRISPR/Cas gene editing technology have significantly expanded the

239 production dramatically, we used CRISPR-Cas9 gene editing technology to knockout (KO) targeted host g

240 y discusses new applications of CRISPR-based gene editing technology with increased efficiency and sp

241 e a large animal model employing CRISPR/Cas9 gene editing technology.

242 xon 4 of HLA-B*38:01:01:01 using CRISPR/Cas9 gene editing technology.

243 ultured luteal cells by CRISPR/Cas9 mediated gene editing technology.

244 CRISPR-Cas9 gene-editing technology has facilitated the generation o

245 gh a combination of in vitro experiments and gene-editing technology, we further demonstrate that ant

246 firmed BMI1 as the direct target of AR using gene-editing technology.

247 signs is to achieve rapid, robust, heritable gene editing, the formation of unintended mutations at o

248 the development of efficient, combinatorial gene-editing therapeutics.

249 ta demonstrate the feasibility of an ex vivo gene-editing therapy for more than 95.6% of dominant KRT

250 have been subjected to CRISPR/Cas9-mediated gene editing, there is no evidence of success in genetic

251 e novel tools for the conditional control of gene editing, thereby enabling the investigation of spat

252 h substantially increases the versatility of gene editing through precise and rapid switching ON or O

253 th anti-CRISPR molecules become resistant to gene editing, thus providing a means to generate "write-

254 ced short palindromic repeats (CRISPR)-based gene editing to characterize COPD-associated regulatory

255 in gene-edited cell populations and expands gene editing to chromosomal tracts previously not possib

256 We used gene editing to confer picrotoxin resistance on delta re

257 This work establishes that precise gene editing to correct multiple distinct gene variants

258 Here we use CRISPR/Cas9 gene editing to create a conditional GR knockdown in Spr

259 Employing CRISPR/Cas9 gene editing to disrupt the Pdgfra gene in two different

260 To address this, we used CRISPR/Cas9 gene editing to generate a novel recessive mouse model o

261 Here, we used CRISPR/Cas9 gene editing to generate AR mutant A. burtoni and perfor

262 tional cohesin-interacting proteins, we used gene editing to introduce a dual epitope tag into the en

263 We previously used gene editing to introduce a dual epitope tag into the en

264 ug target discovery, yet reliable, multiplex gene editing to reveal synergies between gene targets re

265 e, we use a novel method of circuit-specific gene editing to show that the transcription factor Delta

266 We now use gene-editing to insert a fluorogen activating protein (F

267 Previously we used gene-editing to label endogenous EGF receptor (EGFR) wit

268 nt technological approaches, mainly based on gene editing, to produce allogeneic CAR T cells with lim

269 palindromic repeats (CRISPR) as an accurate gene editing tool also indicate that the high-specificit

270 recent publication by Mok et al. describes a gene editing tool capable of installing point mutations

271 CRISPR/Cas is a transformative gene editing tool, that offers a simple and effective wa

272 the mechanisms by which this widely utilized gene-editing tool selects target sites, avoids spurious

273 ops, and with CRISPR/Cas9 being an effective gene-editing tool, here we demonstrate that CRISPR/Cas9-

274 zing A*T to G*C conversions, is an important gene editing toolbox.

275 ce of stem cell biology, bioengineering, and gene editing tools have substantially broadened our abil

276 xpand the applicability of CRISPR-associated gene editing tools in vitro and in vivo.

277 onucleotides or inhibitory RNAs, delivery of gene editing tools such as clustered regularly interspac

278 cent advances in transgenic technologies and gene editing tools, combined with fluorescence-activated

279 concentrates on the emerging versatility of gene-editing tools, which has enabled the emergence of v

280 spliceosome, with potential applications as gene-editing tools.

281 ops, and how these genes can be tested using gene editing, transgenic and TILLING approaches.

282 the-art and promising perspective of in vivo gene editing using non-viral nano-vectors.

283 ptive immune responses and enables effective gene editing using repeated dosing.

284 Recently, CRISPR/Cas9 gene editing was applied to these species; however, a ma

285 CRISPR/Cas9 gene editing was used to correct the pathogenic p.Gly23V

286 To overcome this obstacle, CRISPR-Cas9 gene editing was used to generate mice in which a hemagg

287 CRISPR/Cas9 gene editing was used to produce somatic mutations of OT

288 studies, bisulphite sequencing, ChIP-Seq and gene-editing we show that the p50/p50 homodimer known to

289 Using CRISPR/Cas9-mediated gene editing, we further identified NMIIA as the major i

290 sing forward genetic methods and CRISPR/Cas9 gene editing, we identified and characterized the role o

291 Using CRISPR/Cas9 gene editing, we modeled human L1CAM and CRB2 mediated a

292 known actin-bundling protein) by CRISPR-Cas9 gene editing, we showed that actin bundles are required

293 5(-/-) THP-1 cells (generated by CRISPR-Cas9 gene editing) were incubated with Helicobacter and gene

294 ree dominant Best disease iPSC-RPE models to gene editing, which produced premature stop codons speci

295 ele of Grin1 can be restored to wild type by gene editing with Cre recombinase.

296 The pairing of CRISPR/Cas9-based gene editing with massively parallel single-cell readout

297 l enable routine generation of nucleases for gene editing with no detectable off-target activity.

298 Combining in vivo CRISPR-based CDK4 and CDK6 gene editing with pharmacologic inhibition approaches in

299 this study, we demonstrate highly efficient gene editing within uncultured primary naive murine CD8(

300 oreover, conventional mutation correction by gene-editing would only be relevant for the small fracti