ライフサイエンスコーパス: gene knockout

1 n the early coding region, ensuring faithful gene knockout.

2 valuated the basis of drug susceptibility by gene knockout.

3 s disclosed the targets affected by Galpha12 gene knockout.

4 suppressed by targeted cell ablation and/or gene knockout.

5 ium stipitatum) and investigated by targeted gene knockout.

6 the CB2R gene knockout mice are constitutive gene knockout.

7 characterized from a series of biosynthetic gene knockouts.

8 ed robustness assessments, as in the case of gene knockouts.

9 ation of insertions and deletions typical of gene knockouts.

10 d transcriptomes generated for non-metabolic gene knockouts.

11 RISPR/Cas9 nuclease is commonly used to make gene knockouts.

12 ing the production of the first 1,751 unique gene knockouts.

13 nts that are more severe than in other cilia gene knockouts.

14 -trap-based screen and comparison with yeast gene knockouts.

15 ct of 439 small-molecule challenges on 1,448 gene knockouts.

16 they are generated and model the effects of gene knockouts.

17 tal analysis when phenotyping new individual gene knockouts.

18 e/natriuretic peptide receptor-A, GC-A/NPRA) gene-knockout (0-copy; Npr1(-/-) ), 2-copy (Npr1(+/+) ),

19 Disruption of this binding or GPR110 gene knockout abolishes while GPR110 overexpression enha

20 idence from 85 to 45% (P = 0.038) and miR-31 gene knockout abrogated development of ESCC (P = 1 x 10(

21 een skeletal muscle samples from adiponectin gene knockout, adiponectin-reconstituted, and control mi

22 e of hypoadiponectinemia in GDM, adiponectin gene knockout (Adipoq(-/-) ) and wild-type (WT) mice wer

23 However, adiponectin gene knockout (Adipoq(-/-)) attenuated maternal obesity-

24 15% were achieved with RPT and RMT, and >50% gene knockout after RET.

25 Gene knockout analysis demonstrated that functional coop

26 Gene knockout analysis shows that MadB1-4 contribute to

27 lly, based on AgRP2 peptide localization and gene knockout analysis, we demonstrate that pre-optic Ag

28 3 in CTV pathogenicity was assessed based on gene knockout and complementation in N. benthamiana.

29 ssues or primary adipocytes from adiponectin gene knockout and control mice.

30 PINCER is available for individual gene knockout and genome-wide screening for both the hum

31 nstitutes a unique tool to spatially control gene knockout and greatly broadens the scope of loss-of-

32 The combination of SIRT1 gene knockout and imatinib treatment further extends the

33 ensive use of multiple CRISPR/Cas9-generated gene knockout and knockin cell lines to investigate the

34 eplicating plasmids and suicide plasmids for gene knockout and knockin.

35 n this study, we used mouse models of Kiss1r gene knockout and mouse mammary tumor virus-polyoma viru

36 Gene knockout and over-expression of PpMACRO2 significan

37 didates to be tested in strategies involving gene knockout and overexpression in plants.

38 Previous gene knockout and promoter swap experiments showed that

39 Gene knockout and RNAi in individual neurons reveal that

40 Furthermore, gene knockout and selective pharmacological inhibition o

41 ential functions, but studies using targeted gene knockout and siRNA-mediated transcript knockdown to

42 Using CRISPR-mediated gene knockout and subsequent complementation, we validat

43 utcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutiv

44 Abeta degradation in vivo through the use of gene knockout and transgenic mice.

45 throid miR-144/451 target mRNAs, we compared gene knockout and wild-type fetal liver erythroblasts by

46 Six-week-old CSE gene knockout and wild-type mice were fed with either a

47 ly validated in Arabidopsis thaliana through gene knockout and/or overexpression studies.

48 binding site, (ii) generating translational gene knockouts and (iii) introducing other coding or non

49 Live-cell imaging coupled with targeted gene knockouts and a high-throughput RNAi assay enabled

50 Using BBS gene knockouts and a mouse infection model, we show the

51 observed no significant relationship between gene knockouts and clinical consultation or prescription

52 t ergot alkaloid mutants derived by previous gene knockouts and differing in ergot alkaloid profiles.

53 etermined by RNA sequencing and confirmed by gene knockouts and growth phenotypes.

54 been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells.

55 describes the use of this technique to make gene knockouts and knock-ins, as well as single-nucleoti

56 ptimized to create either single or multiple gene knockouts and large chromosomal deletions.

57 predicting genome-wide gene expression given gene knockouts and master regulator perturbations.

58 ased MYC abundance sensor, CRISPR/Cas9-based gene knockouts and next-generation sequencing.

59 Using data from five single gene knockouts and the wild type strain, we decrease the

60 library of CRISPR/Cas9-based glyco-relevant gene knockouts and their examination in glycomic and fun

61 ed proteomics, structural analysis, targeted gene knockouts and treatment with canavanine demonstrate

62 ion for the phenotypic discrepancies between gene-knockout and gene-knockdown(1,2); however, the unde

63 otein interaction, subcellular localization, gene knockout, and bioinformatics analyses.

64 ing single-cell transcriptomics, CRISPR-Cas9 gene knockout, and genotype-specific donor experiments p

65 f pendrin under conditions not confounded by gene knockout, and to test the potential utility of pend

66 dvances in cell labeling, microscopy, single-gene knockouts, and genomics, as well as the results of

67 By taking a gene-knockout-and-reconstitution approach, we have chara

68 These results suggest that the gene knockout approach may be able reduce antigenicity i

69 dopamine (DA) system, we used a conditional gene knockout approach to selectively delete GRK2 in DA

70 Using a conditional gene knockout approach, the alpha isoform of p38 mitogen

71 Using a CRISPR/Cas9-based gene knockout approach, we identified galectin-3 as the

72 Using a genome-wide gene knockout approach, we identified Keap1, the SWI/SNF

73 Using a tissue-specific gene knockout approach, we inactivated Shp2 expression i

74 to discrepancies between pharmacological and gene knockout approaches and opposite influences on coca

75 which precludes investigation using classic gene knockout approaches.

76 hat rare autosomal and X chromosome complete gene knockouts are important inherited risk factors for

77 ab techniques used for this purpose, such as gene knockout, are high in resource and time consumption

78 ties on various substrates and the effect of gene knockouts at the genome scale.

79 nding protein 4 was strongly reduced in Bmp9 gene knockout (Bmp9-KO) mice as compared with wild-type

80 We evaluated each gene knockout by screening IR-relevant phenotypes in the

81 ructural components of the SPN, and alveolin gene knockout causes morphological abnormalities that co

82 his approach, we generated single and double gene knockout cells within multiple mouse infection mode

83 deletion of expression using the conditional gene knockout (cKO) approach.

84 haracterized Cre-loxP system for conditional gene knockout (CKO) in mice.

85 letion of the yeast Saccharomyces cerevisiae gene-knockout collection (YKOC), which has enabled high-

86 genes whose roles were probed via individual gene knockouts, construction of over-expression strains,

87 designed to mediate either gene knockdown or gene knockout, coupled with next-generation sequencing.

88 ting THIK-1 function pharmacologically or by gene knockout depolarizes microglia, which decreases mic

89 A btl19 -13 gene knockout did not prevent the bacterium from infecti

90 f-2alpha through various combinations of Phd gene knockouts did not further improve hepatic metabolis

91 C lines, as well as stage-specific inducible gene knockout during hPSC differentiation.

92 manner and that existing additive models of gene knockout effects fail at capturing genetic interact

93 cytosine or guanine significantly increases gene knockout efficiency in CRISPR-Cas9-based genome edi

94 The gene knockout efficiency in this system was evaluated ra

95 We have also compared pairwise gene knockout essentiality predictions for 10 of these m

96 es that generate lethal phenotypes in single gene knockout experiments as essential genes.

97 Single-gene knockout experiments can fail to reveal function in

98 om quantitative RT-PCR and CRISPR/Cas9-aided gene knockout experiments indicated that androgen differ

99 ative genomics approaches and the results of gene knockout experiments were used to extrapolate the m

100 complementation tests and CRISPR/Cas9-based gene knockout experiments.

101 Gene-knockout experiments on single-cell organisms have

102 We also conclude that whole-gene "knockouts" fail to reveal how important gene produ

103 al infection using small interfering RNA and gene knockout fibroblasts, implicating cytosolic DNA as

104 stmitotic neurons using in vivo and in vitro gene knockout for the mitochondrial division protein Drp

105 Optimization procedures to identify gene knockouts for targeted biochemical overproduction h

106 entional "selectivity filter." AQP2-specific gene knockout generated MPXR trypanosomes but did not af

107 alpha-Galactosidase A gene knockout (Gla KO) mice have no alpha-galactosidase

108 ld-type (WT), alpha1,3-galactosyltransferase gene-knockout (GTKO), and TKO pig peripheral blood monon

109 ld-type (WT), alpha1,3-galactosyltransferase gene-knockout (GTKO), and TKO pigs by flow cytometry.

110 ineered pigs (alpha1,3-galactosyltransferase gene-knockout [GTKO] pigs and pigs expressing a human co

111 To date, only a few CRISPR/Cas9-mediated gene knockouts have been reported using the chicken as a

112 Over the last approximately 30 y, gene knockouts have revealed that CaV1.1/RyR1 coupling r

113 nt one-step generation of double- and triple-gene knockout hPSC lines, as well as stage-specific indu

114 h-throughput phenotyping of 530 unique mouse gene knockouts identified 140 monogenic 'hits', of which

115 Magnetization-based screening of gene knockouts identified Tco89p, a component of TORC1 (

116 e evaluation of the deubiquitinase family by gene knockout, identifying several deubiquitinases, UCH3

117 potent stem cell (hPSC) lines with inducible gene knockout (iKO) remains challenging.

118 Rdx gene knockout impairs reversal learning and short-term m

119 CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts,

120 ave emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish a

121 Despite efficient gene knockout in embryonic day 14.5 (E14.5) dermal conde

122 s9 components for the generation of targeted gene knockout in mammals.

123 In this study, we show that Ehd1 gene knockout in mice on a predominantly B6 background i

124 aracterized Cre-loxP systems for conditional gene knockout in mice, and studied the effects of these

125 o 1,500-fold in cell lines and to facilitate gene knockout in mouse zygotes without sacrificing on-ta

126 In this paper, we demonstrate efficient gene knockout in the squid Doryteuthis pealeii using CRI

127 The development of CRISPR/Cas9-mediated gene knockout in two ant species opens a new window into

128 e editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

129 al genetic studies through the generation of gene knockouts in a variety of model organisms including

130 ugh the dopamine D2 receptor with the use of gene knockouts in Caenorhabditis elegans or RNA interfer

131 Escherichia coli strains that resulted from gene knockouts in central metabolism followed by metabol

132 nd scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pl

133 e Keio collection, a set of nonlethal single-gene knockouts in E. coli BW25113.

134 d the entire KEIO collection of 3,985 single-gene knockouts in Escherichia coli for increased suscept

135 ology has enabled the rapid establishment of gene knockouts in many cell types and even whole organis

136 Here, using gene knockouts in mice, we evaluated genetic interaction

137 Phenotypic variation among gene knockouts in S. cerevisiae fits a double Pareto-log

138 Here, we generated TERC (telomerase RNA) gene knockouts in telomerase positive cell lines that re

139 ted the effects of different combinations of gene knockout, including arsenate reductase (HAC1), gamm

140 a single-step method where CRISPR/Cas9-based gene knockout is combined with insertion of a pAID plasm

141 y of the Htt gene in CNS development through gene knockout is problematic as the knockout leads to em

142 ection of all viable Escherichia coli single-gene knockouts is facilitating a systematic investigatio

143 Further analysis of NE gene-knockout isolates showed that the effect of some in

144 ced RAP1 activation in WT and integrin beta3 gene knockout (Itgb3-/-) OCs, but its effects were subst

145 RISPR/Cas9 technology for genome editing and gene knockout, its performance still relies on well-desi

146 not occur in alpha1,3-galactosyltransferase gene-knockout kidney xenografts.

147 valuable for spatial and temporal control of gene knockout, knockin, and reporter expression in the m

148 SPR/Cas9 system for generating site-specific gene knockout/knockin models, including a knockout pig m

149 We therefore investigated whether Tric-a gene knockout (KO) alters the single-channel function of

150 Fkbp5 gene knockout (KO) and wild-type (WT) mice were assessed

151 se LARP4 accumulated to higher levels in TTP gene knockout (KO) cells than in control cells.

152 ted MCF7 breast cancer cell line-derived DPH gene knockout (ko) cells to assess the impact of complet

153 Gene knockout (KO) does not always result in phenotypic

154 LEN based methods for accomplishing complete gene knockout (KO) in AML cells.

155 We generated TL1A gene knockout (KO) mice to assess its role in collagen-i

156 pe (WT) mice were compared with those in CSE gene knockout (KO) mice.

157 lected by the previous finding that systemic gene knockout (KO) of Anp32b leads to embryonic lethalit

158 efficiencies on generating Factor VII (FVII) gene-knockout (KO) mice.

159 diseases, we have not yet explored how such gene knockouts (KOs) could influence complex human pheno

160 B. bassiana targeted gene knockouts lacking Bbcyp52x1 displayed reduced virul

161 somal acid lipase (hLAL) expression into LAL gene knockout (lal(-/-)) mice.

162 ion factor pdx1 through CRISPR-Cas9-mediated gene knockout leading to disturbed pancreatic developmen

163 suggest that an increase in EETs due to sEH-gene knockout leads to an increase in the size of islets

164 CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool

165 We fit a large published data set of single-gene knockout lines in Saccharomyces cerevisiae to seven

166 otypic variation in a large series of single-gene knockout lines than other skewed or symmetrical dis

167 Treatment of multidrug resistance 2 gene knockout (Mdr2(-/-) ) mice with A-1331852 resulted

168 s using Polk(-/-) Polh(-/-) Poli(-/-) triple-gene knockout MEFs unexpectedly revealed that another po

169 m represents a highly effective and scalable gene knockout method in zebrafish and has the potential

170 Previous CRISPR gene knockout methods in cell lines have relied on frame

171 ic effects, we analyzed these factors in Ada gene knockout mice (Ada(-/-)).

172 Our previous study reported that adiponectin gene knockout mice (Adipoq (-/-) ) develop GDM due to in

173 smooth muscle-specific acid ceramidase (Ac) gene knockout mice (Asah1(fl/fl)/SM(Cre)) were used to d

174 Using gene knockout mice and in vivo cellular depletion method

175 d lipolysis was observed in both adiponectin gene knockout mice and primary adipocytes from these mic

176 ll as in plasma samples from six cholestatic gene knockout mice and six age- and gender-matched wild-

177 almitoyltransferase (SPT) subunit 2 (Sptlc2) gene knockout mice and sphingomyelin synthase 2 (Sms2) g

178 METHODS AND We used H- and K-Ras gene knockout mice and subjected them to pressure overlo

179 ecific mechanisms are unclear since the CB2R gene knockout mice are constitutive gene knockout.

180 ntribution to its etiology, including single-gene knockout mice associated with diaphragmatic defects

181 However, studies in connexin-gene knockout mice demonstrated significant CV slowing o

182 In C57BL/6 gene knockout mice lacking either functional TCRs or MHC

183 is enhancement was lost almost completely in gene knockout mice lacking either TCRs or MHC class II m

184 ing to echocardiography, we found that Efnb3 gene knockout mice showed enhanced constriction in the c

185 he central corneal epithelium of C57BL/6 and gene knockout mice was abraded, and 1 x 10(7) S. marcesc

186 ood pressure (shared by some but not all BBS gene knockout mice).

187 We generated Necl4 gene knockout mice, but found that disruption of Necl-4

188 ing into question negative results in single-gene knockout mice.

189 ere markedly reduced in TNF receptor 1 and 2 gene knockout mice.

190 s can predict the lethal phenotype of single-gene knockout mice.

191 where possible, testing of islet tissue from gene knockout mice.

192 out mice and sphingomyelin synthase 2 (Sms2) gene knockout mice.

193 rkedly reduced in tamoxifen-inducible Nkx2-5 gene knockout mice.

194 r mice, an adoptive cell transfer model, and gene knockout mice.

195 deletion of CB1, CB2, or GPR55 receptors in gene-knockout mice failed to alter BCP's action against

196 d a streptozotocin-induced DKD model in FHL2 gene-knockout mice to determine the possible role of FHL

197 Individual gene knockouts mirrored many of the observed changes in

198 Using MMP-9 gene knockout (MMP-KO) mice, we investigated the effect

199 Using different murine lymphocyte gene knockout models, we show that the enhanced sensitiv

200 death or are lethal in the orthologous mouse gene knockout models.

201 strategy to generate the whole body dematin gene knockout mouse model (FLKO).

202 We used a pan-DC, CD11c-specific cre-lox gene knockout mouse model to assess the role of KLF2 in

203 Using a gene-knockout mouse model, we demonstrate that the admin

204 h in ATL, xylose metabolism was targeted for gene knockout mutagenesis.

205 of the biosynthetic monomers obtained from a gene knockout mutant (DeltaUV_2091) of V. virens.

206 Expressing the two genes in trans in the two-gene knockout mutant complemented full virulence.

207 Through a series of markerless gene knockout mutants and complementation tests, specifi

208 contributed to host immunity, as Arabidopsis gene knockout mutants displayed quantitatively enhanced

209 rases were determined via SMRT sequencing of gene knockout mutants.

210 Here, using bioinformatics approaches, gene-knockout mutants, purified recombinant proteins, LC

211 Here, we report the generation of gene knockout NRG mice by combining in vitro fertilizati

212 Indeed, gene knockout of Polbeta caused mitochondrial dysfunctio

213 hy MS methods, we have screened 1,755 single gene knockouts of Escherichia coli and have identified t

214 Gene knockouts of the Bacillus C-methyltransferase and t

215 Targeted gene-knockout of Bbmsn2 resulted in reduced growth and i

216 Gene knockouts often yield limited insights into functio

217 macrophages showed that inhibiting ACAT1 by gene knockout or by pharmacological inhibition caused a

218 Mice with global IL-1 receptor gene knockout or central IL-6 receptor knockdown showed

219 ations, has been limited by lack of a robust gene knockout or knock-down technology.

220 Further, either Ido1 gene knockout or pharmacological inhibition of hippocamp

221 asma cells were inherently sensitive to Rab7 gene knockout or Rab7 activity inhibition in class switc

222 Except for DeltaBbCypA, targeted gene knockouts or overexpression of any cyclophilin resu

223 rodent models of arrhythmia caused by clock gene knockouts or surgical ablation of the suprachiasmat

224 PfHsp110c gene-knockout parasites were not viable and conditional

225 Here, we characterize its function using a gene-knockout (Pbyop1Delta).

226 ole of SIGLEC1 in PRRSV infection, a SIGLEC1 gene knockout pig was created by removing part of exon 1

227 After alpha1,3-galactosyltransferase gene-knockout pig artery patch xenotransplantation, reci

228 dy binding to alpha1,3-galactosyltransferase gene-knockout pig cells was less than to wild-type cells

229 ic changes in alpha1,3-galactosyltransferase gene-knockout pig kidneys transplanted into baboons that

230 ografts from alpha 1-3 galactosyltransferase gene knockout pigs, which express human complement regul

231 wild-type and alpha1,3-galactosyltransferase gene-knockout pigs) and anti-Gal IgM and IgG levels (by

232 We treated Pkd1 gene knockout (Pkd1KO) mice with dDAVP, a V2R agonist, f

233 currently the fastest algorithm for finding gene knockouts predicted by FBA to increase production o

234 Gene knockout, protein-YFP tags and physiological assays

235 ribe here a detailed protocol for generating gene knockout rats by homologous recombination in embryo

236 rotocol to generate Tp53 (also known as p53) gene knockout rats.

237 l antibody or tumor necrosis factor receptor gene knockout, reduced inflammation and retinal ganglion

238 survivals of alpha1,3-galactosyltransferase gene knockout renal xenografts in this laboratory correl

239 By establishing a gene knockout-rescue system in a Brd2-null erythroblast

240 rs or specific small interfering RNA or JAK3 gene knockout resulted in an increase in TLR-mediated pr

241 Conditional gene knockout resulted in sex-specific responses wherein

242 lpha, beta, gamma, delta), only pPLAIIIdelta gene knockout results in a decrease in seed oil content,

243 Pcgf3/5 gene knockout results in female-specific embryo lethalit

244 RPE65 gene knockout (Rpe65(-)/(-)) mice showed abolished isome

245 ate paralog pairs using CRISPR/enCas12a dual-gene knockout screening in three cell lines.

246 of the CRISPR-Cas9 system to pooled library gene knockout screens in mammalian cells represents a ma

247 , a supervised learning method for analyzing gene knockout screens.

248 Somatic gene knockouts showed both genes are required for effici

249 e report the successful generation of global gene-knockout, single-amino-acid-substituted, as well as

250 Disrupting TPC function by gene knockout, small interfering RNAs, or small-molecule

251 Unlike wild-type, most gene knockout strains were found to have one additional

252 The optimization problem to derive gene knockout strategies recently has been formulated as

253 Antibody neutralization and gene knockout strategies were used to show that the pres

254 Gene knockout strategies, RNAi and rescue experiments ar

255 e uterine epithelium using a tissue-specific gene knockout strategy, in which floxed Mig-6 (Mig-6(f/f

256 clusions were unattainable by using standard gene knockout studies alone.

257 SPR/Cas9 is a powerful gene editing tool for gene knockout studies and functional genomic screens.

258 intersected with the findings of three siRNA gene knockout studies and identified potential drug targ

259 However, gene knockout studies have reached disparate conclusions

260 Whereas gene knockout studies in mice have shown that both the O

261 ect in vitro enzyme assays, we conducted PGP gene knockout studies in P. berghei, confirming that thi

262 In sum, our results show that single-gene knockout studies of a progenitor virus can have lit

263 Evidence obtained from gene knockout studies supports the role of Toll-like rec

264 Gene knockout studies were previously attempted to explo

265 Our results from gene knockout studies, LSC tracing and transplantation m

266 ously identified as virulence genes in whole-gene knockout studies.

267 eleterious alternative to wild-type Cas9 for gene-knockout studies.

268 Flow cytometry showed that RBC from 2-gene knockout swine exhibited less human antibody bindin

269 pCMV into our alpha1,3-galactosyltransferase gene knockout swine herd.

270 only mimic the in vivo setting, and current gene-knockout technologies do not allow the simultaneous

271 Here we show via gene knockout that whereas DraRnl is inessential for gro

272 and describe naturally occurring homozygous gene knockouts that suggest the dispensability of a vari

273 previously published reports of conditional gene knockouts that used the Trp1-Cre mouse, suggesting

274 Six weeks after Pkd1 global gene knockout, the mice were treated with Agt-ASO (66 mg

275 d as an effective tool for sequence-specific gene knockout through non-homologous end joining (NHEJ),

276 CRISPR/Cas9 is used extensively to engineer gene knockouts through NHEJ, editing by HDR remains inef

277 When TLR4 gene knockout (TLR4(-/-)) and TLR4-competent (TLR4(+/+))

278 d megalin/cubilin-deficient mice produced by gene knockout to determine whether receptor-mediated end

279 on microscopy, single-molecule tracking, and gene knockouts to examine the dynamics of individual Tcp

280 authors used whole-body and tissue-specific gene knockouts to show that Slc30a10 is paramount for Mn

281 , from high-throughput phenotyping of single-gene knockouts under hundreds of annotated conditions, f

282 ncing (RNA-seq) and from RNA interference to gene knockouts using Clustered Regularly Interspaced Sho

283 IV host factors in primary CD4(+) T cells by gene knockout, validation, and HIV spreading infection i

284 to produce RBCs that allow the generation of gene knockouts via CRISPR/Cas9 using the immortal JK-1 e

285 SH gene knockout virus (RSV DeltaSH) is partially attenuate

286 loid-specific cre-expressing mice, effective gene knockout was achieved in various myeloid cells.

287 ut mice were generated and the effect of the gene knockout was determined, including COX activity, ti

288 maging, force measurements, pharmacology and gene knockout, we show that dynamic assembly of filament

289 e-specific neuron silencing, and conditional gene knockout, we show that these molecularly defined 5H

290 ted genes in a cell line panel with presumed gene knockouts, we detect the production of foreign mRNA

291 bining parabiosis, single-cell analyses, and gene knockouts, we uncover a multi-organ web of tissue-r

292 Isotope feeding and gene knockout were employed in the engineered S. lividan

293 As rhoph2 and rhoph3 gene knockouts were not viable in the human P. falciparu

294 lular carcinoma cells containing CRISPR-Cas9 gene knockouts were treated with 15 mM APAP for 30 minut

295 Moreover, elimination of NeuroD1 by gene knockout, while it does not disrupt initial OSN dif

296 5%, and 1% oxygen to systematically identify gene knockouts with relative fitness defects in high oxy

297 oped a streamlined approach for CRISPR-based gene knockouts with three key advantages, which allows p

298 ous approach also enabled a screen combining gene knockouts with transcriptional activation, which re

299 Examining complete gene knockouts within a viable organism can inform on ge

300 advantages, which allows phenotypic assay of gene knockouts without clonal selection and expansion.