ライフサイエンスコーパス: gene silencing

1 of small RNA biogenesis during initiation of gene silencing.

2 chromatin formation and post-transcriptional gene silencing.

3 ntional Argonaute that are both required for gene silencing.

4 ng SPOC to Xist RNA is sufficient to mediate gene silencing.

5 ions of bacillary morphologies consequent on gene silencing.

6 ream pathways for chromatin modification and gene silencing.

7 ting, its activity is required for efficient gene silencing.

8 ates, including incomplete Polycomb-mediated gene silencing.

9 DNA methylation, leading to transcriptional gene silencing.

10 K9me3 modification, which is associated with gene silencing.

11 d DNA methylation (RdDM) and transcriptional gene silencing.

12 ate chromatin compaction and transcriptional gene silencing.

13 e3-decorated facultative heterochromatin and gene silencing.

14 take (<30 min), effective siRNA release, and gene silencing.

15 , are the prime determinants of the speed of gene silencing.

16 in the context of histone deacetylation and gene silencing.

17 Mmi1 complex (EMC) implicated in gametogenic gene silencing.

18 tissue accumulation necessary for productive gene silencing.

19 ) or synthetic transacting siRNA vectors for gene silencing.

20 logical inhibition approach that phenocopies gene silencing.

21 luding small non-coding RNA (ncRNA) mediated gene silencing.

22 ir dysregulation through posttranscriptional gene silencing.

23 matin clustering with concomitant effects on gene silencing.

24 with methylation-associated, tumor-specific gene silencing.

25 ase complex make only minor contributions to gene silencing.

26 oteins in variant surface glycoprotein (VSG) gene silencing.

27 lore the implication of chromatin changes in gene silencing.

28 itioning for heterochromatin maintenance and gene silencing.

29 ted in the Drosophila heart using RNAi-based gene silencing.

30 the microRNA-guided Argonaute 1 complex and gene silencing.

31 theless, HDAC activity contributed to stable gene silencing.

32 etically inherited in cis, leading to stable gene silencing.

33 RNAi triggers, which together result in HBV gene silencing.

34 sponsible for histone H2A ubiquitination and gene silencing.

35 ius, but not HRDE-1, for small RNA-dependent gene silencing.

36 ired to initiate small-RNA-induced heritable gene silencing.

37 C1-C with function of the PRC1 in epigenetic gene silencing.

38 H3K27 (H3K27me3) is implicated in epigenetic gene silencing.

39 ant rate-limiting factors for miRNA-mediated gene silencing.

40 ved in specific transcriptional cascades and gene silencing.

41 d chromatin-modifying activities involved in gene silencing.

42 generational epigenetic inheritance (TEI) of gene silencing.

43 onformation that is thought to contribute to gene silencing.

44 ing an unanticipated mechanism for heritable gene silencing.

45 ttranscriptional and also in transcriptional gene silencing.

46 en that would be necessary for siRNA-induced gene silencing.

47 divisions before H3K27me3 dilution relieves gene silencing.

48 chromatin formation and therefore epigenetic gene silencing.

49 rom viral RNA (virus-derived siRNAs) through gene silencing.

50 ic target of Rg3 using molecular docking and gene silencing.

51 enic modification of plants for constitutive gene silencing.

52 long-sought-after mechanism for host-induced gene silencing.

53 of Xist, a region critical for Xist-mediated gene silencing.

54 mmunity, as determined by overexpression and gene silencing.

55 ved multigene family of regulators mediating gene silencing across eukaryotes.

56 s decoy activity and seed sequence-dependent gene silencing activity.

57 NG knockout did not decrease tRF-3 levels or gene-silencing activity.

58 , SNAs act as single-entity transfection and gene silencing agents and have been used as lead therape

59 achieve effective delivery of siRNA-mediated gene silencing agents in vivo.

60 ly alternating 3' U and G nucleotides become gene-silencing agents.

61 gh acquisition of novel alleles that enhance gene silencing, aiding accelerated adaptation.

62 Gene silencing also revealed that Snail enhanced the per

63 of transcription, splicing, translation, and gene silencing, among many others.

64 NA-dependent RNA polymerases responsible for gene silencing amplification.

65 idate RNAi responses in SPB by mortality and gene silencing analysis.

66 Using gene silencing and a selection of microRNA mimics, we co

67 Using this approach, we measure gene silencing and activation for thousands of domains.

68 tic cell memory, including roles controlling gene silencing and cell differentiation.

69 Microrchidia (MORC) ATPases are critical for gene silencing and chromatin compaction in multiple euka

70 reduced by protein turnover and irreversible gene silencing and commitment can occur.

71 e structures that accommodate transcription, gene silencing and DNA replication.

72 This was confirmed by specific LCK gene silencing and ex vivo combined treatment of cells f

73 This condensate is required for gene silencing and for the anchoring of Xist to the Xi t

74 successes in AAV-mediated gene replacement, gene silencing and gene editing have helped AAV gain pop

75 mediates autosomal maternal allele-specific gene silencing and has an important role in imprinted XC

76 pathway functions as an important module in gene silencing and heterochromatin formation.

77 Our study delineates EMC requirements in gene silencing and identifies an ERH interface required

78 Using gene silencing and inhibitor treatments, we determined t

79 study, using shRNA- and CRISPR/Cas9-mediated gene silencing and knockout approaches, along with 3'-ex

80 ohort of these agents inhibited HP1-mediated gene silencing and one lead compound potently inhibited

81 Here, using gene silencing and overexpression approaches, RNA-Seq, a

82 e cell death from oxidative stress, based on gene silencing and pharmacological inhibition in hPSC-CM

83 Using gene silencing and pharmacological modulators in vitro a

84 the roles that Xist repeats A and B play in gene silencing and Polycomb recruitment.

85 T that precludes histone turnover to promote gene silencing and preserve epigenetic stability of hete

86 hermore, PRC2-targeted therapeutics overcome gene silencing and promote tumor clearance by cytotoxic

87 e known to cause important phenomena such as gene silencing and rapid morphological changes.

88 NAUTE1 (AGO1) to direct post-transcriptional gene silencing and regulate numerous biological processe

89 vesicles based on short hairpin RNA (shRNA) gene silencing and the colocalization of LAMP-1, glycopr

90 o-evolved with the gain of ORC-Sir4-mediated gene silencing and the loss of RNA interference.

91 Here, using siRNA-mediated gene silencing and various human cells, we report that U

92 ylation, this change was not associated with gene silencing and was essentially absent in AMLs with D

93 ounteract antiviral responses, including the gene-silencing and innate immunity machineries.

94 t dataset of plants, several rules governing gene silencing, and a series of computational models of

95 A combination of phylogenetic analyses, gene silencing, and biochemical analyses coupled with st

96 a mild reduction of miRNAs levels, impaired gene silencing, and characteristic morphological defects

97 S, site-directed mutagenesis, siRNA-mediated gene silencing, and chemical inhibitors, we identified t

98 ing protein translation, Argonaute-dependent gene silencing, and more.

99 ith functional assays, Ca(2+) imaging, siRNA gene silencing, and pharmacological agonists and antagon

100 says, co-immunoprecipitation, siRNA-mediated gene silencing, and reporter gene assays, we demonstrate

101 ntitumor potential was evaluated considering gene silencing, apoptosis, histopathology and survival o

102 Using a CRISPR interference-mediated gene silencing approach, here we demonstrate that clpC1

103 Gene silencing approaches confirmed the regulatory role

104 romoter hypermethylation events that lead to gene silencing, are associated with a number of human di

105 Ser/Thr kinase (AKT) at Ser(473) siRNA-based gene-silencing assays with primary osteoblasts revealed

106 , calcium FLIPR, and short hairpin RNA-based gene-silencing assays.

107 Altered histone modifications implicated in gene silencing associate with aberrant autoantigen expre

108 Arabidopsis SAC3B dysfunction causes gene silencing at transgenic and endogenous loci, accomp

109 -defense arms race centered on trans-kingdom gene silencing between hosts and pathogens.

110 distinct chromatin modifications to enforce gene silencing, but how transcriptional repression is pr

111 RNA interfering is a eukaryote-specific gene silencing by 20~23-nucleotide (nt) microRNAs and sm

112 Gene silencing by chromatin compaction is integral to es

113 ggest that MORCs affect genome structure and gene silencing by forming multimeric assemblages to topo

114 standing of these mechanisms with a focus on gene silencing by H-NS, transcription coordination by HU

115 Gene silencing by Polycomb complexes is central to eukar

116 Greater understanding of gene silencing by promoter hypermethylation in anal carc

117 pUG tails promote gene silencing by recruiting RNA-dependent RNA polymeras

118 Gene silencing by RNA interference (RNAi) has emerged as

119 the ugtL promoter region, where it overcomes gene silencing by the heat-stable nucleoid structuring p

120 Gene silencing by the SETD5 complex regulates known drug

121 amily of scaffold proteins shown to regulate gene silencing, cell growth, and differentiation.

122 PRC2 is critical for epigenetic gene silencing, cellular differentiation and the formati

123 chnical approaches, including siRNA-mediated gene silencing, ChIP assays, global metabolomics and foc

124 erved chromatin binding proteins involved in gene silencing, chromosome packaging, and chromosome seg

125 ulates various cellular processes, including gene silencing, chromosome segregation, and maintenance

126 is a tightly packed form of DNA involved in gene silencing, chromosome segregation, and protection o

127 nal expression studies in TNBC models, EPHA2 gene-silencing combined with EPA significantly reduced c

128 complex genes (an ovarian steroid-regulated gene silencing complex) in untreated LCLs from women wit

129 several approaches, including siRNA-mediated gene silencing, confocal microscopy, and subcellular fra

130 ed for at least 6 months, with the degree of gene silencing correlating to levels of guide strand tis

131 1, unlike its close homolog Sir3 involved in gene silencing, does not appear to discriminate between

132 t mRNAs without causing post-transcriptional gene silencing, due to its inability to interact with GW

133 r the complex combinatorial rules underlying gene silencing during X inactivation.

134 RNA element located in its 5' end, to induce gene silencing during X-chromosome inactivation.

135 siS100A4@Liposome, and exhibited outstanding gene-silencing effects that significantly inhibited the

136 epatic delivery, but tissue accumulation and gene silencing efficacy are lower than that achieved in

137 Chimeric TMO analogues demonstrate high gene silencing efficacy, comparable to that of a chimeri

138 -siRNA-complexed NPs exhibited excellent GFP gene silencing efficiency in GFP-MDA-MB-468 TNBC cells w

139 The in vitro and in vivo gene silencing efficiency values that have been reported

140 ernalization into plant cells and subsequent gene silencing efficiency.

141 These gene-silencing events are thought to be detrimental to t

142 Gene silencing experiments targeting alpha1D reduced the

143 es of siRNA-L2 facilitated significant tumor gene silencing for 7 d after two i.v. doses.

144 e the SPOC domain as a major effector of the gene-silencing function of SPEN, and show that tethering

145 We use gene silencing, gene expression analysis, genetic mappin

146 e of interdisciplinary approaches, including gene silencing, grafting, transmission electron microsco

147 are believed to remain constant, overcoming gene silencing has largely been ascribed to proteins tha

148 ugh folate receptor mediated endocytosis for gene silencing has not, if any, been successful in clini

149 y, we assessed the potential of Host-Induced Gene Silencing (HIGS) approach to target the Colletotric

150 e current understanding of this host-induced gene silencing (HIGS) process as a defense mechanism dur

151 Validation by virus-induced gene silencing identified two MYB transcription factors,

152 human lung cancer cell lines, siRNA-mediated gene silencing, immunoblotting, quantitative RT-PCR, pro

153 the virus, our results showed that antiviral gene silencing imposes selection in viral populations.

154 s of Fetuin-A (FetA) were conducted by using gene silencing in a mouse asthma model, human dendritic

155 etween the mitochondrial respiratome and VSG gene silencing in bloodstream form T. brucei.

156 V2R gene silencing in Caki-1 cells also reduced cAMP and ERK

157 st RNA to Spen and results in strictly local gene silencing in cis.

158 In vitro gene silencing in cultured endothelial cells was perform

159 ering RNAs (siRNAs) exhibit accumulation and gene silencing in extrahepatic tissues, providing an opp

160 of three different cancers with simultaneous gene silencing in flank and metastatic mouse models of o

161 LDL) metabolism was studied in 3 cell models-gene silencing in HepG2 cells, patient fibroblasts, and

162 ISPR-mediated gene editing and RNAi-mediated gene silencing in human cells, here we analyzed the cons

163 as negated by receptor antagonists and GPR55 gene silencing in L6 myotubes.

164 flow cytometry analyses, and siRNA-mediated gene silencing in leukemia cell lines, we show that AICA

165 These conjugated siRNAs enable functional gene silencing in lung, muscle, fat, heart and adrenal g

166 cargo carrier for direct siRNA delivery and gene silencing in mature plants.

167 ree and force-independent siRNA delivery and gene silencing in mature plants.

168 Deletion of XRN1 impaired gene silencing in N. castellii, and this impaired silenc

169 ranules may similarly contribute to germline gene silencing in other organisms.

170 d with LGR6 overexpression and diminished by gene silencing in phagocytes.

171 cribed here, could become valuable tools for gene silencing in plants with practical applications in

172 ne silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modifi

173 ted DNA methylation-mediated transcriptional gene silencing in plants.

174 for highly effective, specific, and nontoxic gene silencing in plants.

175 MicroRNAs are effector molecules of gene silencing in RNAi, and their modulation can lead to

176 dispensable for the heterochromatin-mediated gene silencing in S. pombe ChIP analysis revealed that V

177 a key regulator in heterochromatin-mediated gene silencing in S. pombe.

178 (di-siRNA), that supports potent, sustained gene silencing in the central nervous system (CNS) of mi

179 RNA design may enable RNA interference-based gene silencing in the CNS for the treatment of neurologi

180 By genetically removing gene silencing in the plant and silencing suppression in

181 NAs) between hosts and pathogens can lead to gene silencing in the recipient organism, a mechanism te

182 enomic region correlated with impaired Avr1b gene silencing in these mutants.

183 scape strategies to overcome transcriptional gene silencing in vegetative tissues contributing to the

184 th requirements that mirror yeast epigenetic gene silencing in vivo.

185 from mouse brains, we demonstrated that LRP1 gene silencing increases expression of proinflammatory m

186 FGFR inhibition (or gene silencing) interrupts stromal autocrine growth and

187 lyses of different tissues and virus-induced gene silencing is an efficient way to identify host prot

188 Heterochromatic gene silencing is an important form of gene regulation t

189 The efficiency of gene silencing is highly variable across genes, with som

190 In contrast to current views, we find that gene silencing is incompletely set early in embryogenesi

191 Mechanisms by which locus-specific gene silencing is initiated and heritably maintained dur

192 duplex strands that comprise the SNA lead to gene silencing is not understood.

193 plex 2 (PRC2), which plays a crucial role in gene silencing, is inactivated through recurrent mutatio

194 We have quantified chromosome-wide gene silencing kinetics at the level of the nascent tran

195 trastructural analysis revealed that exocyst gene silencing led to the striking appearance of novel e

196 the post-transcriptional and transcriptional gene silencing machinery.

197 transcription factor pNFkappaB p65, and the gene silencing marker dimethylated histone H3K9.

198 translational modifications and identify two gene-silencing marks, H3K9me3 and H4K20me3, with relativ

199 RNA interference (RNAi) is a highly specific gene-silencing mechanism that can cause rapid insect mor

200 by transcriptional enhancers and epigenetic gene silencing mediated by Polycomb group proteins.

201 The combined approach of gene silencing, metabolomics, real time expression analy

202 between DNA methylation, a model epigenetic gene silencing modification, and autoantigen gene expres

203 ng activity and histone methylation-mediated gene silencing of ABI5.

204 Gene silencing of Bmp4 or its downstream mediator Smad7,

205 Furthermore, gene silencing of c3g partly rescued nephrocyte diaphrag

206 Gene silencing of Fbxl2 in skeletal myoblasts resulted i

207 exists for compounding hypermethylation and gene silencing of hemizygous tumor suppressor genes (TSG

208 tor activation was responsible for mediating gene silencing of IL-27p28 and EBV-induced gene 3.

209 RNAi-mediated gene silencing of il17a in fibrotic mice arrested the pr

210 Here, we demonstrate that gene silencing of KIND1 decreased keratinocyte prolifera

211 Pharmacologic inhibition or gene silencing of MK2 in vitro abrogated RT-induced incr

212 noncoding RNA Xist mediates chromosome-wide gene silencing of one X Chromosome in female mammals to

213 Virus-induced gene silencing of SlFAD2-7 in spr2 results in significan

214 In vivo, nephrocyte-specific gene silencing of sns or c3g compromised nephrocyte filt

215 nectary of the growing spur tip, and triple gene silencing of the three STY-like genes revealed that

216 tomic data demonstrates extensive epigenetic gene silencing of the transcription factor PRDM16 in ren

217 emental PEMFs, whereas small interfering RNA gene silencing of TRPM7 did not, coinciding with data th

218 Additionally, virus-induced gene silencing of two trichomes preferentially expressed

219 In this study we examined the impact of Tks4 gene silencing on the functional activity of primary hum

220 e show that SPEN is essential for initiating gene silencing on the X chromosome in preimplantation mo

221 mily protein, SmcHD1, which is important for gene silencing on Xi, contributes to this unique chromos

222 ys in mMSCs was carried out in vitro through gene silencing or chemical inhibition of key components.

223 Interestingly, gene silencing or editing experiments revealed that SNAT

224 ro and in vivo, an effect abrogated by USP11 gene silencing or the inhibition of enzymatic activity.

225 Results from gene silencing/overexpression approaches, electrophoreti

226 RNA interference (RNAi) is a gene-silencing pathway that can play roles in viral defe

227 endogenous genes controlled by the germline gene-silencing pathways.

228 eign genetic elements by small RNA-dependent gene-silencing pathways.

229 e PCR, immunoblotting, ELISA, siRNA-mediated gene silencing, plasmid-mediated gene overexpression, la

230 rentially regulate epigenetic maintenance of gene silencing, potentially enabling dynamic heritable r

231 euchromatic and heterochromatic lncRNA-based gene silencing processes.

232 e find that deletion of SIR3, a subtelomeric gene silencing protein, inhibits silencing of subtelomer

233 to manipulate specific cells and tissues for gene silencing, protein overexpression, or genome modifi

234 he human body contains a set of programmable gene-silencing proteins named Argonaute.

235 significant increase in posttranscriptional gene silencing (PTGS) activity.

236 hybrida) are caused by post-transcriptional gene silencing (PTGS) of the key enzyme of anthocyanin b

237 their translation (i.e. post-transcriptional gene silencing, PTGS).

238 h could influence phenotype through unstable gene silencing rather than DNA change(6,7).

239 effects of horizontally acquired MGE-encoded genes, silencing recessive alleles if the recipient bact

240 ular bodies (MVBs) by chemical inhibitors or gene silencing reduced MDV titers and cell-to-cell sprea

241 R antagonists OPC31260 and Tolvaptan, or V2R gene silencing reduced wound closure and cell viability

242 iptional shutoff a state is reached in which gene silencing remains stable even if Xist is turned off

243 ately, isolation of plants undergoing potent gene silencing requires laborious design, visual screeni

244 6 and AqARF8 transcripts using virus-induced gene silencing resulted in largely petal-specific defect

245 inhibiting MLC1 in GSCs using RNAi-mediated gene silencing results in diminished growth and invasion

246 RNA interference (RNAi)-mediated gene silencing revealed that a certain subtype of ILP, G

247 Gene silencing revealed that NaJAZi functions as a flowe

248 Gene silencing revealed that STAT4 was required for IL-6

249 ethods, including short hairpin RNA-mediated gene silencing, RNA and ChIP sequencing analyses, and me

250 several approaches, including RNAi-mediated gene silencing, RNA-Seq- and quantitative RT-PCR-based t

251 nalysis with fusion proteins, siRNA-mediated gene silencing, RT-PCR, and knockout mice, we investigat

252 G6PD inhibition and gene silencing (small interfering RNA) abolished the ben

253 is motivated by recent reports of efficient gene silencing specifically in hepatocytes by small inte

254 behavioral, chemogenetic, and viral-mediated gene silencing strategies and high-resolution microscopy

255 RNA-sequencing screening coupled with gene silencing studies identified ATF3 as the driver of

256 heterogeneous siRNA cocktails to be used in gene silencing studies.

257 SPEN rapidly disengages from chromatin upon gene silencing, suggesting that active transcription is

258 methylation (RdDM)-mediated transcriptional gene silencing (TGS) is a natural antiviral defense agai

259 that SPs are central chromatin regulators of gene silencing that establish immune cell identity and f

260 gene expression in trans via piRNA-mediated gene silencing that is essential for embryonic developme

261 MicroRNAs regulate post-transcriptional gene silencing through base-pair binding on their target

262 RC1 (cPRC1), but not variant PRC1, maintains gene silencing through cell division upon reversal of te

263 ults establish H1 as a critical regulator of gene silencing through localized control of chromatin co

264 Gene silencing through RNAi has been used successfully i

265 DNA methylation abnormalities and associated gene silencing, through inhibiting DNA methyltransferase

266 sing a small kinase inhibitor and RNAi-based gene silencing to disrupt EPHB4 activity, we found that

267 EZHIP 'oncohistone-mimic', that dysregulate gene silencing to promote tumorigenesis.

268 microRNA that mediates post-transcriptional gene silencing to regulate a wide range of biological pr

269 In this study, we used siRNA mediated gene silencing to understand the osteogenic differentiat

270 x 2 (PRC2), a key inducer of transcriptional gene silencing, to uncover silencers, their molecular id

271 r intense investigation over recent years as gene silencing tools.

272 ted in various cellular processes, including gene silencing, translation, stress granule assembly, ce

273 me analysis to assess genome-wide off-target gene silencing triggered by the fragments that were desi

274 This is surprising, as defective gene silencing underlies developmental abnormalities and

275 nvenient and widespread use of virus-induced gene silencing (vectors 2.0).

276 sates are the physical sites of PcG-targeted gene silencing via formation of facultative heterochroma

277 ito Aedes aegypti promotes sequence-specific gene silencing via the expression of two PIWI-interactin

278 Virus-induced gene silencing (VIGS) and transient expression of Phytop

279 Through virus-induced gene silencing (VIGS) in a related crop species, maize (

280 Virus induced gene silencing (VIGS) is a method of transient gene sile

281 Virus-induced gene silencing (VIGS) is used extensively for gene funct

282 rand RNA and expressed through virus-induced gene silencing (VIGS) or synthetic transacting siRNA vec

283 t (MeAPL1-MeAPL5) and employed virus induced gene silencing (VIGS) to show that MeAPL3 is the key iso

284 ing a cotton cDNA library in a virus-induced gene silencing (VIGS) vector.

285 encing by RNA interference and virus-induced gene silencing (VIGS), and X-ray crystallography for str

286 Using Virus Induced Gene silencing (VIGS), we demonstrate that knockdown of

287 he interactions in planta, and virus-induced gene silencing was used for functional characterization

288 erived cardiomyocytes (hiPSC-CMs) and MAP4K4 gene silencing, we demonstrate that death induced by oxi

289 tness of factors in initiating PIWI-directed gene silencing, we employed a Piwi-interacting RNA (piRN

290 Using small pharmacological inhibitors and gene silencing, we established an association between MD

291 After gene silencing, we evaluated the role of each target on

292 scence staining, qRT-PCR, and siRNA-mediated gene silencing, we show that unlike in the canonical pat

293 aracterized roles in active transcription or gene silencing were in highly or poorly labeled forms, r

294 Genetic mapping and viral-induced gene silencing were used to identify immune signaling co

295 Additionally, virus-induced gene silencing, which is RNA mediated and triggered thro

296 in facultative heterochromatin assembly and gene silencing while leaving Mmi1-mediated transcription

297 ker makes DNA contacts that are required for gene silencing, while chromosome compaction does not app

298 nal activation as well as the maintenance of gene silencing, while H2AX is important in DNA damage re

299 opportunities via hormonal manipulation and gene silencing with antisense oligonucleotides.

300 Gene silencing with virally delivered shRNA represents a