ライフサイエンスコーパス: gene targeting

1 and the desired target locus (aptamer-guided gene targeting).

2 us tropicalis which is used for genetics and gene targeting.

3 G3 were disrupted using CRISPR/Cas9-mediated gene targeting.

4 y was sensitive enough to evaluate multiplex gene targeting.

5 strand break, which has been used to promote gene targeting.

6 method can be used for efficient multiplexed gene targeting.

7 gene therapy, if we can achieve RGC specific gene targeting.

8 acile vectors for high-efficiency, multiplex gene targeting.

9 onstrated a high-efficiency in site-specific gene targeting.

10 or resistance to DNA damaging agents and for gene targeting.

11 n cells, often limiting the effectiveness of gene targeting.

12 nt of the TIN2 K280E DC allele (TIN2(DC)) by gene targeting.

13 rry either human COMT Val or Met alleles via gene targeting.

14 ndwork for future studies using aptamers for gene targeting.

15 ransgene to achieve collecting duct-specific gene targeting.

16 of specific epigenetic marks and peripheral gene targeting.

17 ring technology with the ease of multiplexed gene targeting.

18 cific DNA double-strand break, and stimulate gene targeting.

19 for evolution and potential applications in gene targeting.

20 +, we easily achieve previously unattainable gene targeting.

21 transgene into one copy of chromosome 21 by gene targeting.

22 into the 3'-untranslated region of C5ar1 by gene targeting.

23 ens when both HY2 and PUBS were disrupted by gene targeting.

24 ebrafish using zinc-finger nuclease-mediated gene targeting.

25 fic TRAF6 deficiency using Cre/LoxP-mediated gene targeting.

26 BAergic inputs are challenged by conditional gene targeting.

27 quantitative and qualitative nature of STAT-gene targeting.

28 erapies and limit the efficacy of individual gene targeting.

29 st, Cas9/dCas9-PB chimeras did not result in gene targeting.

30 , and causation was confirmed by CRISPR/Cas9 gene targeting.

31 ne TALE-PB chimera demonstrated notable HPRT gene targeting.

32 es even 1 year after tamoxifen-induced Cox10 gene targeting.

33 ed genome rearrangements, and to DSB-induced gene targeting.

34 a safe harbor suitable for nuclease mediated gene targeting.

35 ements in mRNAs are leveraged as signals for gene targeting.

36 floxed mice that can be used for conditional gene-targeting.

37 Here, we show that gene targeting 2'-O-methyl (2'OMe) gapmer antisense olig

38 analysis as a consequence of miRNA-directed gene targeting, a phenomenon that could be exploited to

39 The level of gene targeting achieved was sufficient to correct the pr

40 single-guide RNAs (sgRNAs) and from limited gene targeting activity.

41 acrophages from Gsdmd(-/-) mice generated by gene targeting also exhibit defective pyroptosis and int

42 Cultured NPCs are amenable to gene targeting and can form nephron organoids that engra

43 of 53BP1 at sites of DNA damage and improved gene targeting and chromosomal gene conversion with eith

44 We used gene targeting and computational modeling to test this n

45 monstrated that STAT5 content is crucial for gene targeting and EPC fate.

46 Light Reporter, which provides a readout of gene targeting and gene disruption downstream of a targe

47 s out single-strand annealing in addition to gene targeting and gene disruption.

48 ctors makes them important also as tools for gene targeting and genome editing.

49 Over the last 20 years, however, advances in gene targeting and genome modification in rodent models

50 e IDH1 mutation and ACACA in leukaemia using gene targeting and patient-derived xenografts.

51 stem can be exploited as a powerful tool for gene targeting and precise genome editing in plants.

52 sing retroviral vectors are low frequency of gene targeting and random integration of the targeting v

53 Using gene targeting and RNAi, we showed that depletion of the

54 of Cell Stem Cell, Pak et al. (2015) combine gene targeting and stem cell technologies to identify a

55 finition of ILC2s via IL-7r-conditional Rora gene targeting and takes advantage of a distinct progres

56 bor-intensive embryonic stem (ES) cell-based gene targeting and tedious micromanipulations of mouse e

57 tion domains, we modify the codons for mouse gene targeting and viral production.

58 Live imaging, gene targeting, and cell-cycle inhibitors reveal that cl

59 ential clonal events: initial reprogramming, gene targeting, and subsequent removal of a selection ca

60 cost-effective approach for high efficiency gene targeting applications in CRISPR and TALEN compatib

61 tein engineering-many are unsatisfactory for gene targeting applications.

62 efined factors could spur the development of gene-targeting applications in non-dividing cells.

63 gonal CRISPR-Cas systems in rapidly emerging gene-targeting applications.

64 To test this possibility, we used a gene targeting approach to determine whether the closely

65 inant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mut

66 te the role of BAP1 in DSB repair, we used a gene targeting approach to knockout (KO) this deubiquiti

67 Using a dual-guide gene targeting approach, biallelic deletions in the CCR5

68 Using a Cre/loxP-mediated conditional gene targeting approach, Smad4 gene function was deleted

69 Using an in vivo gene targeting approach, we demonstrate that Rac2, but n

70 Using an efficient gene targeting approach, we developed a novel mouse line

71 Using the conditional mouse gene targeting approach, we show that mice with inactiva

72 Hoxa5 expression domains using a conditional gene targeting approach.

73 Here, we developed a highly efficient gene-targeting approach in the monarch using zinc-finger

74 Here, we employ a novel gene-targeting approach that exploits a specific chromat

75 ce that lack kinase activity using a knockin gene-targeting approach that preserves normal protein ab

76 In the present study, by using the gene-targeting approach to delete TNFRII in AD transgeni

77 We used a conditional gene-targeting approach to identify the relative contrib

78 Using a gene-targeting approach where the mouse PrP coding seque

79 Using a gene-targeting approach, we knocked-in a single copy of

80 Using a gene-targeting approach, we observed that in contrast to

81 temness and pluripotency of murine ESCs by a gene-targeting approach.

82 Through gene targeting approaches in zebrafish and mice, we show

83 Analysis of PhIL1 function using gene targeting approaches indicated that the protein is

84 Here we used inducible and stage-specific gene targeting approaches to elucidate the role of PDK1

85 tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of po

86 pportunity to validate drug-based therapies, gene-targeting approaches and cell replacement strategie

87 Although gene-targeting approaches and phenotype analysis have le

88 forms of parkinsonism are required, now that gene-targeting approaches for Parkinson disease have rea

89 forms of parkinsonism are required, now that gene-targeting approaches for Parkinson's disease have r

90 Traditional gene-targeting approaches in many experimental organisms

91 By using gain- and loss-of-function gene-targeting approaches, here we show that PTEN is cru

92 e history of HD and valuable information for gene-targeting approaches.

93 transcriptional complex but was crucial for gene targeting, as p21(Waf) gene promoter, unlike cyclin

94 We also provide proof-of-principle data that gene-targeting ASOs can be selected to synergize with TL

95 eduled DNA synthesis and a CRISPR-Cas9-based gene-targeting assay.

96 stimulate homologous recombination-mediated gene targeting at a variety of loci, including genes kno

97 Here, using gene targeting at both endogenous CENP-A alleles and gen

98 repair pathway, increases the efficiency of gene targeting at fin1+ to around 75-80% (a 16-fold incr

99 ly, we demonstrate that Cas9 nickases induce gene targeting at frequencies comparable to native Cas9

100 Both TALENs and CRISPR/Cas9 achieved gene targeting at similar efficiencies.

101 wever for some loci, such as fin1+, rates of gene targeting below 5% can limit the scope and scale of

102 uses Helper sites concurrently with WREs for gene targeting, but it also targets TCF1 to sites that d

103 Gene targeting by 'ends-out' homologous recombination en

104 Gene targeting by homologous recombination or by sequenc

105 Gene targeting by repeated local administration of oligo

106 Gene targeting by zinc-finger nucleases in one-cell embr

107 The frequency of injection-mediated gene targeting can be further increased with CRISPR-indu

108 that multiplex embryo transfer and multiplex gene targeting can be used to quickly and efficiently ge

109 Termed CRISPR-Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated CRI

110 Here, using a combination of gene targeting, chromatin profiling, and single-cell RNA

111 eted for REST in progenitors by conventional gene targeting does not exhibit these phenotypes, likely

112 ingly, the overexpression of IL-22 by either gene targeting (e.g., IL-22 transgenic mice) or exogenou

113 mal production, and sheds light on improving gene-targeting efficiencies on pluripotent stem cells.

114 l concept and research direction to increase gene targeting efficiency and lays the groundwork for fu

115 Combining the two improvements increases gene targeting efficiency even at reduced HDR template d

116 J with SCR7 does not increase HDR or improve gene targeting efficiency further, indicating that HR is

117 t minimizes library size without sacrificing gene targeting efficiency.

118 fector nuclease technology, we achieved high gene-targeting efficiency in AAT-deficiency patient iPSC

119 ng/discovery for liver diseases, and the low gene-targeting efficiency in human iPSCs warrants furthe

120 r targeting vector design will inform future gene targeting efforts involving multi-kilobase gene seg

121 Here we describe experiments using gene targeting, electrophysiology, and optical imaging t

122 simple, efficient and scalable way to enrich gene targeting events and to identify the cause of failu

123 ila that increases the number of independent gene targeting events while at the same time enriching f

124 10% of the injected F0 animals transmitting gene-targeting events through their germline.

125 t to be identified mediators of nick-induced gene targeting exist.

126 Although recent gene targeting experiments demonstrated that the presenc

127 Gene targeting experiments have shown that the cytokine

128 Unfortunately, with particularly challenging gene targeting experiments, our original design yielded

129 er cells phenocopies the effects observed in gene targeting experiments.

130 The present gene-targeting experiments demonstrate that mouse embryo

131 Subsequently, CDK11 siRNA gene targeting, expression profiling, and network recons

132 avored bipolar fate outcomes, whereas double gene targeting favored photoreceptor fate.

133 gene-driven approach that comprises specific gene targeting followed by phenotypic assessment.

134 matic cells, restricting the use of standard gene targeting for most laboratory and clinical applicat

135 in genetically engineered mouse models, and gene-targeting for the generation of knock-in mice.

136 arms and chromosomal target sequences reduce gene targeting frequencies in several species; however,

137 ased on the bean yellow dwarf virus enhanced gene targeting frequencies one to two orders of magnitud

138 d base pair can significantly decrease human gene targeting frequencies.

139 nhance ZFN-mediated targeted mutagenesis and gene targeting (GT) in Arabidopsis by manipulating DNA r

140 quency, selectable marker-free intra-genomic gene targeting (GT) in maize.

141 The ability to edit plant genomes through gene targeting (GT) requires efficient methods to delive

142 c endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different spe

143 Conditional gene targeting has been extensively used for in vivo ana

144 Although gene targeting has been used extensively to modify the g

145 ng engineered nucleases to stimulate precise gene targeting have also demonstrated correction of dise

146 Powerful methods of gene targeting have helped to decipher gene-function ass

147 ategies routinely use cDNA-based vectors for gene targeting; however, inclusion of noncoding componen

148 ic mosquitoes carrying antipathogen effector genes targeting human malaria parasites and to generate

149 We used gene targeting in a disease-susceptible rat model of gen

150 otein and short guide RNA, driving efficient gene targeting in a non-integrative manner.

151 cytes in vivo, Cre-loxP-mediated conditional gene targeting in adult mice was used.

152 own mice were generated by Cre/LoxP-mediated gene targeting in ApoE(-/-) mice.

153 TALEN-mediated gene targeting in avian PGCs is therefore an efficient p

154 More generally, gene targeting in avian primordial germ cells will foste

155 is method may allow for safe and efficacious gene targeting in both infants and adults by greatly dim

156 y of sophisticated genetic modifications via gene targeting in both mouse and human NSC lines, includ

157 finger nuclease (ZFN) technology facilitates gene targeting in diverse species and cell types, but an

158 e to be a viable alternative to conventional gene targeting in embryonic stem cells where simple loss

159 The advantages of gene editing versus gene targeting in embryonic stem cells, including the br

160 -engineered strains made via transgenesis or gene targeting in embryonic stem cells.

161 Gene targeting in ES cells was used to create an EGFP kn

162 Here we provide a detailed protocol for gene targeting in human cells with AAV vectors.

163 mology-directed DNA repair pathway constrain gene targeting in human HSCs.

164 n iPSC-based disease model and highly robust gene targeting in human iPSCs, both of which are critica

165 ein (CDX2P-CreER(T2)) to allow for inducible gene targeting in intestinal epithelium.

166 caffeine treatment has been shown to prevent gene targeting in mammalian cells by increasing non-prod

167 oxa3 was the first Hox gene to be mutated by gene targeting in mice and is required for the developme

168 eloping recombinase-assisted and conditional gene targeting in mice as a main technical tool.

169 Gene targeting in mice subsequently demonstrated that th

170 within a single lineage, we used conditional gene targeting in mice to ablate Sin3a from perinatal qu

171 Here, we use gene targeting in mice to determine the requirement of G

172 In this study, we used gene targeting in mice to identify the in vivo functions

173 Traditional gene targeting in mice using embryonic stem (ES) cells,

174 findings demonstrate the accuracy of in vivo gene targeting in modeling human cancer and suggest futu

175 Using conventional gene targeting in mouse embryonic stem cells, we report

176 cleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms.

177 ficity at two diverse targets, and stimulate gene targeting in multiple cell types including human in

178 ships of the different domains of DEK1 using gene targeting in null mutant background.

179 ntional methods are not readily adaptable to gene targeting in other cell types.

180 Cd9 gene targeting in PECs prevents glomerular damage in CGN

181 vercome the efficiency barrier that has made gene targeting in plants challenging.

182 the first knockin rat model made by germline gene targeting in spermatogonial stem cells.

183 he preceding decades from forward screens to gene targeting in stem cells to the recent influx of CRI

184 system, which is widely used for conditional gene targeting in the mouse.

185 ng of p22(phox) reduces HIF-2alpha-dependent gene targeting in vitro and tumor formation in vivo.

186 lly generated an RFX6(HA) reporter allele by gene targeting in wild-type H9 cells to precisely define

187 blications have demonstrated the efficacy of gene targeting in zebrafish using CRISPR/Cas9, and they

188 Here we show that inactivation of Cul4a by gene-targeting in mice only affected male but not female

189 Custom endonuclease-based gene targeting involves two mechanisms of DNA repair: ho

190 Gene targeting is a genetic technique to modify an endog

191 Gene targeting is a protocol for introducing a mutation

192 Gene targeting is an incredibly valuable technique.

193 This genetic correction strategy without gene targeting is potentially applicable to any dominant

194 is of hair follicle epithelial stem cells by gene targeting is well established, the molecular and ge

195 been established by cell type-specific il-10 gene targeting, it remains elusive to what extent IL-10

196 ily engineered and highly effective tool for gene targeting; it has considerable off-target effects i

197 Our findings demonstrate how precise gene targeting may be applied to correction of X-CGD usi

198 Both chemical- and gene-targeting-mediated inactivation of Hh signaling aug

199 genetic tool advantageous over conventional gene targeting methods.

200 Using in vitro and in vivo gene-targeting methods, we show that Ipo11 loss results

201 To develop a new microglia gene targeting model, we first applied massively paralle

202 By characterizing a conditional gene targeting mouse model bearing T cell deletion of Rh

203 P1R12C (AAVS1) alleles in CD8(+) T cells and gene targeting of a GFP transgene cassette in >40% of CD

204 Zinc finger nuclease-mediated gene targeting of a single-copy gp91(phox) therapeutic m

205 This study shows that conditional gene targeting of beta-catenin in the dorsal neural fold

206 Gene targeting of betacyto-actin, but not gammacyto-acti

207 Here, we show that sequential gene targeting of embryonic stem cells can be used to yi

208 Yet no gene targeting of Erk1/2 in muscle fibers in vivo has be

209 e of the JCI, Beedle et al. used conditional gene targeting of Fktn, the gene responsible for Fukuyam

210 Conventional gene targeting of Jak2, creating a null allele, leads to

211 However, gene targeting of matrilin-1 in mouse did not lead to pr

212 Site-specific gene targeting of PSIP1 may have therapeutic potential f

213 In addition, gene targeting of t-FcepsilonRIbeta attenuated microtubu

214 Conditional gene targeting of the catalytic subunit of glutamate cys

215 opmental arrest when individually ablated by gene targeting or knockdown.

216 imply eliminating a specific gene product by gene targeting or RNA interference.

217 This work studies gene targeting patterns amongst miRNAs with differential

218 Using a gene-targeting plasmid containing a loxP-flanked drug-re

219 sequencing using newly designed DEH 16S rRNA gene targeting primers.

220 Further, reprogramming and typical gene-targeting protocols can be readily performed on the

221 human CD4+ T cells raises the possibility of gene targeting PSIP1 combinatorially with CCR5 for HIV-1

222 termed 'meganucleases' that are employed as gene-targeting reagents.

223 Because gene targeting remains significantly enriched, relative

224 of the wild-type IDH1 allele in IMA cells by gene targeting resulted in an 87-fold decrease in cellul

225 Such pathogen gene-targeting RNAs represent a new generation of enviro

226 n activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimul

227 lements reveals their chromatin features and gene-targeting specificity.

228 1 pathway genetically in mice by conditional gene targeting starting 8 weeks after spinal hemisection

229 ular vector construction and high-efficiency gene-targeting strategies have been combined to mutate g

230 ul use within antisense, antigene, and other gene-targeting strategies.

231 several disease-specific iPSCs using various gene-targeting strategies.

232 In summary, the miRNA-based viral gene targeting strategy described here allows us to labe

233 profile data, we expect that the miRNA-based gene targeting strategy presented here can help delineat

234 urons, labeled via a novel miRNA-based viral gene targeting strategy, combinatorial to traditional pr

235 cells can be significantly influenced by the gene-targeting strategy and genetic background employed.

236 hippocampal tissue and designed a Dlg4/PSD95 gene-targeting strategy: a Dlg4/PSD95 zinc finger DNA-bi

237 n the AGRIN signaling pathway cause CMS, and gene targeting studies in mice confirm the importance of

238 ds to be considered in the interpretation of gene targeting studies in the RPE.

239 Recent mouse gene targeting studies of rhoA and its regulators/effect

240 Gene targeting studies revealed that Dicer1 is required

241 e-expressing mice as controls in conditional gene targeting studies.

242 Mouse gene-targeting studies have provided convincing evidence

243 Gene-targeting studies in mice have identified the essen

244 Consistent with in vivo gene-targeting studies in mice, both gene- and transcrip

245 Gene-targeting studies in mice, in vitro analyses of hum

246 se limitations, we designed a new, inducible gene-targeting system by introducing an in-frame nonsens

247 c nephropathy, we used an inducible Cre-loxP gene-targeting system that enabled genetic deletion of V

248 Using an adenovirus-mediated gene targeting technique, we attempted to create an HCT1

249 Herein, we used gene-targeting techniques to delete PAT in Plasmodium yo

250 o improve safety, including the emergence of gene targeting technologies for the treatment of PIDs.

251 were discovered in chickens, yet the lack of gene targeting technologies in birds has limited biomedi

252 significantly more efficient than any other gene-targeting technology that is currently available an

253 /Cas system has been adapted as an efficient gene-targeting technology with the potential for multipl

254 3-cre knock-in allele to perform conditional gene targeting, testing the GDNF coreceptors Gfra1 and R

255 sis, JAK/STAT signaling, we used conditional gene targeting to develop a fully penetrant small animal

256 ur knowledge, the first successful selective gene targeting to epithelial vs. mesenchymal cells in an

257 e generated humanized DISC1-Boymaw mice with gene targeting to examine the in vivo functions of the f

258 al feature of pericytes, we used conditional gene targeting to examine the von Hippel-Lindau/prolyl-4

259 the organization of taste circuits, we used gene targeting to express the transsynaptic tracer barle

260 Here we have used in vivo gene targeting to insert an enhancer-promoter element at

261 of Cdk2 inhibitory phosphorylation, we used gene targeting to make an endogenous Cdk2 knockin allele

262 f GalNAc-T6 in colon cancer, we used precise gene targeting to produce isogenic colon cancer cell lin

263 odel system using zebrafish transgenesis and gene targeting to provide an explanation for this phenot

264 odel system using zebrafish transgenesis and gene targeting to provide an explanation for this phenot

265 on specifies TRAV15/DV6 as a Vdelta, we used gene targeting to replace the promoter region of a TRAV1

266 ate whether this might be true, we performed gene targeting to selectively delete LXRalpha in hepatoc

267 For example, gene targeting to the ROSA26 locus by homologous recombi

268 We used gene targeting to validate candidate genetic factors.

269 tamer-containing oligonucleotides stimulated gene targeting up to 32-fold in yeast Saccharomyces cere

270 for his contributions to the development of gene targeting using homologous recombination in embryon

271 bout homology-directed repair (HDR)-mediated gene targeting using long donor DNA templates in hPSCs w

272 Conditional gene targeting using the bacteriophage-derived Cre recom

273 lation needed to make a conditional knockout gene targeting vector to only two steps: a single round

274 Using a gene-targeting vector or a synthetic oligodesoxynucleoti

275 co-delivered with an appropriately designed gene-targeting vector, they can stimulate gene replaceme

276 recombineering tool kit for generating mouse gene targeting vectors and demonstrate the advantage of

277 rovides a tool for the design and display of gene targeting vectors in the same genome browser, along

278 d expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat

279 produced through IVF, and a high rate of Fah gene targeting was achieved with microinjection of Cas9

280 nuclease-mediated DNA double-strand breaks, gene targeting was promoted by replication of the repair

281 Conditional gene targeting was used in mice to explore the role of P

282 Through gene targeting we find that Lnx1 deficiency led to a hip

283 Using mouse models of Raptor or Rictor gene targeting, we discovered that Rictor ablation inhib

284 power of congenic mapping and nuclease-based gene targeting, we established a system where a pair of

285 Using conditional gene targeting, we found that GC-specific inactivation o

286 Using efficient gene targeting, we generated a precise PpDEK1 deletion (

287 By efficient gene targeting, we have replaced, en masse, the promoter

288 By gene targeting, we introduced a point mutation (W1206R)

289 Here, using conditional gene-targeting, we show that the canonical mediator of T

290 Molecules that elevate HDR may improve gene targeting whereas inhibiting molecules can be used

291 However, gene targeting, which is crucial for functional studies

292 he RUNX1 mutation in 1 FPD iPSC line through gene targeting, which led to normalization of megakaryop

293 he Nobel Prize in medicine for their work on gene targeting, which showed that embryonic stem cells c

294 PpRAD50 strongly and specifically inhibited gene targeting, whilst rates of untargeted transgene int

295 Gene targeting with adeno-associated virus (AAV) vectors

296 Here we combined gene targeting with an auxin-inducible degradation syste

297 Combining gene targeting with chromatin immunoprecipitation sequen

298 yme function, and validates the use of GALNT gene targeting with SimpleCells for broad discovery of d

299 ssociated virus (rAAV)-mediated promoterless gene targeting without nucleases and demonstrate amelior

300 Conventional embryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcrip