ライフサイエンスコーパス: general base catalysis

1 rates, and the results are inconsistent with general base catalysis.

2 role for a second active site carboxylate in general base catalysis.

3 His-124 to be unprotonated, appropriate for general base catalysis.

4 the water is activated by metal ligation and general base catalysis.

5 tructure in addition to the critical role of general base catalysis.

6 up a candidate for activation of S10 through general base catalysis.

7 ate EAr hydrolysis may be the result of lost general base catalysis.

8 m the presence of K42 for transimination and general base catalysis.

9 epsilon-amino group of Lys 345 functions in general base catalysis.

10 212, respectively) provided general acid and general base catalysis.

11 to bypass the defect through intramolecular general base catalysis.

12 a) consistent with this nucleotide acting in general base catalysis.

13 GeNb removes DMMP, and thus likely GB/GD, by general base catalysis: a) the k(H2 O)/k(D2 O) solvent i

14 f a calcium-bound water molecule promoted by general base catalysis and close contacts with substrate

15 a mechanism for GDPMH is proposed involving general base catalysis and electrostatic stabilization o

16 racin pathways are significantly enhanced by general base catalysis and that this broadly expands the

17 o activate the hydrolytic water molecule via general base catalysis and to deliver this proton to the

18 CH(3)OH --> CH(2)O by an o-quinone requires general-base catalysis and the reaction is endothermic.

19 ctivation by NaBH3CN, which does not require general base catalysis, are similar to that for the nati

20 rboxylation of mandelylthiamin is subject to general base catalysis (beta = 0.26), an outcome that is

21 ion site in AP has been suggested to provide general base catalysis, but comparison of AP with an evo

22 ls, reveals key mechanistic information: (a) General base catalysis by a carboxylate coupled to gener

23 However, general base catalysis by a ribozyme has not been demons

24 y conserved active site Cys and general acid-general base catalysis by a stringently conserved active

25 , we propose a concerted mechanism involving general base catalysis by Asp64 and electrophilic stabil

26 AP) to d-glyceraldehyde phosphate (GAP), via general base catalysis by E165.

27 n at the anomeric C1 atom of the sugar, with general base catalysis by H124.

28 ns with the solvent, an effect attributed to general base catalysis by the carboxylates.

29 ost likely mechanism involves intramolecular general base catalysis by the neighboring amine group.

30 esults, an isomerization mechanism involving general-base catalysis by a low pKa proline-1 and electr

31 g antibody catalysts for processes for which general-base catalysis can be exploited.

32 ning formation of this intermediate involves general base catalysis, defined by concerted proton tran

33 231 (pKa of 8, seen in Kcat), which provides general base catalysis for addition of H2O to the zinc-a

34 e presence of Mg2+ as a cofactor, indicating general base catalysis for the nucleophilic attack of hy

35 Two different proposals for general base catalysis have emerged from these structura

36 ng group pKa substrates, and the presence of general base catalysis in E-P hydrolysis.

37 ase is able to utilize both general acid and general base catalysis in each direction of the reaction

38 lysis in the cleavage pathway should provide general base catalysis in ligation.

39 activity, supporting a role for general acid/general base catalysis in spiroacetal formation.

40 sal of low barrier hydrogen bond-facilitated general base catalysis in the addition of Ser 195 to the

41 acceptor specificity and assess the need for general base catalysis in the deacylation transition sta

42 ate was examined to reveal the importance of general base catalysis in the enzymatic reaction.

43 The enzyme-like efficiency of general base catalysis in this system directly reflects

44 ervations support the view that general acid-general base catalysis, inefficient in simple model syst

45 ydroxyl reactivity is broadly facilitated by general base catalysis involving multiple RNA functional

46 , but neither phosphate torsional strain nor general base catalysis is evident.

47 r enzymes that require both general acid and general base catalysis likely require reverse protonatio

48 e describe how both reaction pathways, via a general base catalysis mechanism and via direct cleavage

49 thiolate-imidazolium ion pair and suggest a general base catalysis mechanism during transpeptidation

50 The reaction appears to operate under a general base catalysis mechanism, instigated by the beta

51 alkaloid-catalyzed alcoholysis proceeds by a general base catalysis mechanism.

52 The calculations indicate a general base catalysis mechanism.

53 7 as a catalytic base, although evidence for general base catalysis mediating deprotonation of Cys fr

54 The data are consistent with general base catalysis of the postoxidation events yield

55 propionate side chain of Glu-461 to provide general base catalysis of the reaction of water with the

56 ain participates directly in general acid or general base catalysis of the wild-type ribozyme to faci

57 Such an absence of general base catalysis of water attack is consistent wit

58 The proposed reaction mechanism involves general-base catalysis of the attack by active site nucl

59 DeltaG++ = 1.1 kcal/mol for Asp-297-CO(2)(-) general-base catalysis of Wat1 hydration of the immediat

60 General base catalysis supplied by the histidine-12 (H-1

61 ine attacks the carbonyl carbon of PNAA with general-base catalysis to form a substantially tetrahedr

62 ollows a rate law that is characteristic for general base catalysis which, in principle, is consisten

63 eutral conditions, the dominant mechanism is general base catalysis with water serving as the proton