ライフサイエンスコーパス: genetic engineering

1 rve as the basis for revolutionary tools for genetic engineering.

2 nd allows component reusability with minimal genetic engineering.

3 of carotenoid production in crop plants via genetic engineering.

4 ge (SBOL) Visual is a graphical standard for genetic engineering.

5 elopment as a powerful and versatile tool in genetic engineering.

6 d guided improvements via in-silico-assisted genetic engineering.

7 ia, and thus constitutes a powerful tool for genetic engineering.

8 d therefore represent a difficult target for genetic engineering.

9 utation is recapitulated in a mouse model by genetic engineering.

10 ur have largely relied upon various forms of genetic engineering.

11 ntially powerful class of tools for targeted genetic engineering.

12 T cells, and its translation to therapeutic genetic engineering.

13 iple-step biosynthetic pathways using simple genetic engineering.

14 ting H. pylori mutant construction and other genetic engineering.

15 range of biological models and not requiring genetic engineering.

16 ction, or for practical applications through genetic engineering.

17 scovery paved the way for the development of genetic engineering.

18 gineering, either using natural variation or genetic engineering.

19 fixation, the use of fluorescent markers, or genetic engineering.

20 FNs) are powerful tools for gene therapy and genetic engineering.

21 icity concerns, plant disease treatment, and genetic engineering.

22 ic genome and has become a powerful tool for genetic engineering.

23 ld be attenuated at the early liver stage by genetic engineering.

24 osynthetic enzymes with proteins obtained by genetic engineering.

25 ds for specific gene insertion for precision genetic engineering.

26 gi using both environmental manipulation and genetic engineering.

27 er, thereby limiting the efficiency of plant genetic engineering.

28 kinetics of ureolytic microorganisms through genetic engineering.

29 ell as for crop improvement programs and for genetic engineering.

30 ility to rapidly construct novel vectors for genetic engineering.

31 ies, and SLA class I is a possible target of genetic engineering.

32 tools in the fields of molecular biology and genetic engineering.

33 e tracing to any eukaryotic organism without genetic engineering.

34 broader application to all cells amenable to genetic engineering.

35 With the advent of genetic engineering, a highly functional population of T

36 making these enzymes interesting targets for genetic engineering aimed at increased hydrogen producti

37 a powerful and indispensable tool for plant genetic engineering and agricultural biotechnology.

38 biological methods such as systems biology, genetic engineering and bio-refining for optimizing alga

39 Finally, genetic engineering and cell-specific application of ext

40 Genetic engineering and DNA manipulation techniques enab

41 f microbial evolution, interaction networks, genetic engineering and drug discovery.

42 Genetic engineering and evolutionary adaptation to incre

43 es of the PB system for applications such as genetic engineering and gene therapy.

44 ic perspective on selected topics related to genetic engineering and genome editing.

45 nts modified by modern biotechniques, namely genetic engineering and genome editing.

46 imultaneous, an effect with implications for genetic engineering and horizontal gene transfer.

47 g field of engineered macrophages, including genetic engineering and integration with biomaterials or

48 Recent developments in genetic engineering and intravital microscopy have allow

49 Using genetic engineering and isotope labeling experiments in

50 rimarily driven by technological advances in genetic engineering and metabolism as well as by the rea

51 insertion mechanisms will inform methods of genetic engineering and plant transformation.

52 characterize relapse-inducing cells, we used genetic engineering and proliferation-sensitive dyes in

53 By taking advantages of the genetic engineering and self-assembly, we propose the si

54 With the aid of genetic engineering and subsequent mutant analysis, the

55 oscience novels use selected technologies of genetic engineering and synthetic biology to create ente

56 ting challenges of the current dCas tools in genetic engineering and synthetic biology, and provide p

57 many tools that have advanced the fields of genetic engineering and synthetic biology.

58 s glycerol in MECs and provide insights into genetic engineering and system design approaches that ca

59 r immunology and revealing new challenges in genetic engineering and target selection.

60 d immunotherapy, highlighting discoveries of genetic engineering and therapeutic use.

61 Advances in genetic engineering and understanding of pathogenesis ha

62 structural and functional manipulation using genetic engineering and/or bio-orthogonal chemistries.

63 ch as recombineering (recombination-mediated genetic engineering) and DNA gap repair typically involv

64 dvances in the selection of optimal T cells, genetic engineering, and cell manufacturing are poised t

65 levels were manipulated by external sprays, genetic engineering, and drought to reveal their role in

66 xtinction through selective breeding without genetic engineering, and fuels the topical controversy o

67 biotherapeutic development, tissue-specific genetic engineering, and genetic disease prediction will

68 Advancements within biotechnology, genetic engineering, and synthetic chemistry have opened

69 mbineering is an efficient method of in vivo genetic engineering applicable to chromosomal as well as

70 A new genetic engineering approach was developed whereby varia

71 Direct, somatic, genetic engineering approaches allow for accelerated and

72 Current genetic engineering approaches almost exclusively employ

73 rtunities for MYB transcription factor-based genetic engineering approaches of grass biomass.

74 is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal

75 es of oral bacterial delivery, from internal genetic engineering approaches to external encapsulation

76 lation in energy crops, and lead to rational genetic engineering approaches to modify lignin for impr

77 ng the quality of lignocellulosic biomass by genetic engineering approaches.

78 Receptor-specific proteins produced by genetic engineering are attractive as PET imaging agents

79 of plant genetic improvement, including crop genetic engineering, are in place, and are expected to p

80 e 1976 "swine flu affair," possibilities for genetic engineering as an alternative to poultry vaccina

81 o revolutionize our view of systems biology, genetic engineering as well as disease mechanisms and mo

82 The human skin is a promising conduit for genetic engineering, as it is the largest and most acces

83 ation of in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a po

84 Their protein subunits can be modified by genetic engineering at predetermined positions, allowing

85 Further, the DNA vectors enable genetic engineering at reduced costs and eliminate risks

86 stic to different genetic designers, termed 'genetic engineering attribution', would deter misuse, ye

87 Jaeger to engage with the public on GMOs and genetic engineering broadly.

88 designed nucleases (CDNs) greatly facilitate genetic engineering by generating a targeted DNA double-

89 Genetic engineering by homology-directed repair (HDR) is

90 Toward this goal, genetic engineering can be used to make protoxin express

91 athways, through horizontal gene transfer or genetic engineering, can have unpredictable consequences

92 We combined yeast genetic engineering, chemical biology, and multiwaveleng

93 By combining genetic engineering, chromatin biochemistry, and single-

94 for engineered cell-based therapies in which genetic engineering could enhance therapeutic efficacy a

95 bryonic stem cells (mESCs) are key tools for genetic engineering, development of stem cell-based ther

96 Recent genetic engineering developments need to be adapted and

97 eps by exploiting the powerful techniques of genetic engineering, directed evolution, and biomimetics

98 iting technology mediated by CRISPR has made genetic engineering easier than ever, both in vitro and

99 This work has also inspired genetic engineering efforts aimed at generating crops wi

100 These findings will facilitate breeding and genetic engineering efforts to incorporate this new sour

101 Genetic engineering enables the insertion or replacement

102 tope tag in recombinant CD95L variants or by genetic engineering-enforced formation of hexamers.

103 ces the consequences of inherited mutations, genetic engineering, environmental and genetic perturbat

104 The past few decades have seen the field of genetic engineering evolve at a rapid pace, with neurosc

105 architecture, and will have implications in genetic engineering experiments, gene therapy, and under

106 A review of the recent progress in plant genetic engineering for disease resistance highlights fu

107 g transport and its potential application in genetic engineering for increasing pathogen resistance a

108 use as a facile and powerful means of plant genetic engineering for scientific and agricultural appl

109 mmunotherapy, and can be further enhanced by genetic engineering for targeted immunotherapy.

110 ttes at base-level precision is transforming genetic engineering from a laborious art to an informati

111 e many classes of genomic novelties; natural genetic engineering functions are regulated and subject

112 ents; cells can target the action of natural genetic engineering functions to particular genome locat

113 the loss of homeostatic control over natural genetic engineering functions.

114 We review current, likely, and potential genetic engineering (GE) applications for the developmen

115 1016 1016 References 1016 Genetic engineering (GE) can be used to improve forest p

116 ontinued advances in fundamental immunology, genetic engineering, gene editing, and synthetic biology

117 Thus, as T cell genetic engineering has become clinically relevant, we a

118 Genetic engineering has developed around technologies en

119 Plant genetic engineering has made significant contributions t

120 Genetic engineering has potential to enrich diets signif

121 Mouse genetic engineering has revolutionized our understanding

122 manization of animal model immune systems by genetic engineering has shown great promise for antibody

123 cines in plants, especially fish vaccines by genetic engineering, has not yet been addressed.

124 Recent advances in genetic engineering have enabled the delivery of clinica

125 nthetic biology, advances in immunology, and genetic engineering have made it possible to generate hu

126 Advances in genetic engineering have overcome these limitations by i

127 ology; microbiology, immunology, and medical genetics; engineering, imaging, and synthetic chemistry;

128 become one of the most widely used tools for genetic engineering in eukaryotes.

129 -specific silencing of genes may be used for genetic engineering in mice and has possible therapeutic

130 Genetic engineering in mice enables the time-controlled

131 Using genetic engineering in mice, we have generated and chara

132 Genetic engineering in pigs holds a great promise in bio

133 ed, some of which represent the most complex genetic engineering in plants to date.

134 ice of the most appropriate T-SSR system for genetic engineering include that, whenever possible, the

135 y whole genome sequencing (WGS) can identify genetic engineering, including the introduction of antim

136 Genetic engineering is a molecular biology technique tha

137 In the search for renewable energy sources, genetic engineering is a promising strategy to improve p

138 Plant genetic engineering is an important tool used in current

139 tacking in trait development process through genetic engineering is becoming complex with increased n

140 Genetic engineering is being applied to eliminate or sub

141 Plant genetic engineering is routinely performed with the path

142 rosis can be achieved in animals by germline genetic engineering, leading to hypercholesterolemia, bu

143 n-binding motif into a canonical one through genetic engineering led to a stable reddish rubredoxin.

144 conifer stems through genomic selection and genetic engineering may increase resistance to bark beet

145 The rapidly improving tools of genetic engineering may make it possible to overcome the

146 omethionine instead of natural methionine by genetic engineering methods.

147 of a pre-existing template as in traditional genetic engineering methods.

148 e T cell reactivity synthetically or through genetic engineering might thus be translated into new th

149 ntres; cells have a broad variety of natural genetic engineering (NGE) functions for transporting, di

150 An array of methods based on genetic engineering now complements older methods such a

151 Genetic engineering now enables the design of live viral

152 Here we describe the genetic engineering of a "chimeric" HPr/NPr protein, ter

153 The genetic engineering of a biophysical CCM into land plant

154 CAR T-cell treatment involves genetic engineering of a patient's T cells by insertion

155 ication in agriculture, for instance through genetic engineering of ABA receptors.

156 Therefore, AAV-mediated genetic engineering of adipose tissue represents a usefu

157 se a major barrier to DNA transformation and genetic engineering of bacterial species.

158 Genetic engineering of cis-regulatory elements in crop p

159 ocardial repair is significantly enhanced by genetic engineering of CPCs with Pim-1 kinase.

160 This is relevant for the genetic engineering of crops with economic interest that

161 From domestication and breeding to the genetic engineering of crops, plants provide food, fuel,

162 n to its therapeutic potential, chemical and genetic engineering of Dps will offer a nanoplatform to

163 The genetic engineering of EPSPS led to the introduction of

164 We discovered that genetic engineering of Fas variants impaired in the abil

165 ring gene therapy trials have shown that the genetic engineering of haematopoietic stem and progenito

166 Genetic engineering of hCPCs with Pim-1 enhanced repair

167 The genetic engineering of hematopoietic stem cells is the b

168 in microbes as well as genomic fragments for genetic engineering of higher eukaryotes.

169 We have also recently shown that the genetic engineering of human dendritic cells (DCs) to ex

170 These are based on genetic engineering of human genomic safe harbors combin

171 Targeted genetic engineering of human pluripotent cells is a prer

172 pplication of ZFN technology to the targeted genetic engineering of human pluripotent stem cells and

173 HEGs could be used to take the step from the genetic engineering of individuals to the genetic engine

174 troduce an experimental system that combines genetic engineering of kinase activity and quantitative

175 Genetic engineering of lignocellulosic biomass has previ

176 microalgae points to new possible avenues of genetic engineering of lipid metabolism in this organism

177 f genome modifications, their application to genetic engineering of livestock has been slowed by tech

178 We expect that further genetic engineering of M13 viruses can allow us to coass

179 cient use of ARTs, facilitating their use in genetic engineering of macaque monkeys for basic and tra

180 gence of novel pathways in nature as well as genetic engineering of microbes for "green" manufacturin

181 l for providing the foundation necessary for genetic engineering of microorganisms for industrial use

182 Genetic engineering of model organisms and cultured cell

183 Combining genetic engineering of MPK4 activity and quantitative pr

184 rogram population-level dynamics through the genetic engineering of multiple cooperative strains poin

185 d and quality through molecular breeding and genetic engineering of new cotton cultivars.

186 ained in this study will facilitate rational genetic engineering of NRPS to generate unnaturally meth

187 es and has potentially broad applications in genetic engineering of oleaginous crops and microorganis

188 The aECM formed through self-assembly and genetic engineering of phage can be used to understand t

189 describe a comprehensive protocol to perform genetic engineering of phage, liter-scale amplification,

190 Genetic engineering of plants for natural products can h

191 Genetic engineering of plants is at the core of sustaina

192 y of promoter sequences is necessary for the genetic engineering of plants, in basic research studies

193 describes the state of the art in multigene genetic engineering of plants.

194 he genetic engineering of individuals to the genetic engineering of populations.

195 genetic engineering of promastigotes for cytosolic accum

196 lated tissues can help in the development of genetic engineering of resistance against this pathogen.

197 However, current delivery methods for the genetic engineering of single cells, including viral tra

198 and antibody-binding patterns and introduce genetic engineering of SLA epitopes.

199 Recent progress in genetic engineering of spider silks and the development

200 Genetic engineering of stem cells yields augmented thera

201 Safe genetic engineering of stem cells, using facile methods

202 Genetic engineering of structure-designed bromodomain an

203 Twenty-five years after its inception, the genetic engineering of T cells is now a therapeutic moda

204 ter gene expression and involves the ex vivo genetic engineering of T lymphocytes with a reporter gen

205 Genetic engineering of the biosynthetic pathway in the p

206 Further genetic engineering of the GAd capsid holds the promise

207 Rapid and stable genetic engineering of the liver would allow systematic,

208 Genetic engineering of the mouse genome identified many

209 primate, if these can be resolved by further genetic engineering of the organ-source pigs, a pig live

210 es mild, aqueous conditions with no required genetic engineering of the proteins.

211 er nuclease (ZFN) technology has enabled the genetic engineering of the rat genome.

212 d to primary tissue and does not require any genetic engineering of the respective sample sources.

213 fied which can assist in rapid and efficient genetic engineering of these bacteria in the future.

214 r activity, demonstrating the feasibility of genetic engineering of these cells.

215 Here we report rapid and targeted genetic engineering of this parasite using zinc-finger n

216 pora tropica by mutagenesis to allow for the genetic engineering of unnatural derivatives of the pote

217 at will expand the available toolbox for the genetic engineering of vector control technologies.

218 ces making them potentially suitable for the genetic engineering of wild-type populations of animals

219 In addition to genetic engineering, oligo-induced mutagenesis may have

220 obial catalysts via culturing approaches and genetic engineering or adaptive evolution, make this pla

221 escribed methods, GlycoFRET does not require genetic engineering or antibodies to label receptors.

222 Targeting these factors via genetic engineering or breeding approaches can allow dyn

223 a novel strategy for extending shelf life by genetic engineering or conventional breeding.

224 cal aspects such as modifying bacteria using genetic engineering or directed evolution, mass culturin

225 This study establishes a precise genetic engineering platform for genetic studies of hMSC

226 and provided novel insights into the natural genetic engineering processes involved in evolution.

227 Genetic engineering projects often require control over

228 Large genetic engineering projects require more cistrons and c

229 d on average, which could hinder large-scale genetic engineering projects.

230 nderstanding algal metabolism and developing genetic engineering protocols.

231 Genetic engineering provides an alternative approach to

232 Genetic engineering provides an ingenious method of atte

233 d combining single-cell transcript counting, genetic engineering, real-time imaging and computational

234 ntrinsic disruption of Fas signaling through genetic engineering represents a potentially universal s

235 enoids in plastids through plant breeding or genetic engineering requires an understanding of the lim

236 Improvements in plant photosynthesis by genetic engineering show considerable potential towards

237 ch as recombineering (recombination-mediated genetic engineering), single DNA break repair (SDBR, int

238 optimise fermentation conditions and improve genetic engineering strategies among others.

239 implications of concentration robustness in genetic engineering strategies and medical applications.

240 us cell wall will help to steer breeding and genetic engineering strategies for the development of su

241 to N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this n

242 In this study, we demonstrate genetic engineering strategies to overcome these shortco

243 to overcome the limitations of conventional genetic engineering strategies.

244 e importance of macrophage mitoOS, we used a genetic engineering strategy in which the OS suppressor

245 Functional and genetic engineering studies with human commensals, coupl

246 and efficient gene editing than all previous genetic engineering systems.

247 years as a result of recent improvements in genetic engineering techniques and the availability of m

248 lly, we highlight the latest developments in genetic engineering techniques and their possible applic

249 Advances in tissue and genetic engineering techniques are expected to improve t

250 However, conventional genetic engineering techniques target the nuclear genome

251 Genetic engineering technologies can be used both to cre

252 A limited number of analogs produced by genetic engineering technologies show reduced cytotoxici

253 immunotherapies, which could be coupled with genetic engineering technologies to meet specific clinic

254 h high-throughput genotyping, sequencing and genetic engineering technologies, and information reposi

255 iology underlying ripening and the advent of genetic engineering technologies, researchers have pursu

256 ing attitudes about a medical application of genetic engineering technology (gene therapy).

257 Later, the spread of genetic engineering technology enabled investigators to

258 NOD ES cells are unsuitable for the complex genetic engineering that is required to improve human he

259 Staudinger ligation, NCA polymerisation, and genetic engineering, that have been used to great effect

260 ough Agrobacterium can be popularly used for genetic engineering, the influence of aboveground insect

261 The advent of genetic engineering-the ability to edit and insert DNA i

262 escribe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitut

263 to tune the preferred pH of bioactuators by genetic engineering, these biohybrid microsystems could

264 g both sophisticated chemistry and extensive genetic engineering, this technology provides a convenie

265 We examined the feasibility of utilizing genetic engineering to circumvent the restricted capacit

266 plant detoxification systems and the use of genetic engineering to combine bacterial explosives-deto

267 approaches must be adopted involving use of genetic engineering to enhance resistance to cell death

268 s theory of natural selection with bacterial genetic engineering to generate a biological system with

269 l approaches and has emerged as a target for genetic engineering to improve lignocellulose digestibil

270 We used genetic engineering to improve mineral micronutrient con

271 ave inspired novel approaches based on using genetic engineering to manipulate vector populations and

272 een a major focus of both plant breeding and genetic engineering to meet the global demand for food,

273 nsferred cardiac progenitor cells (CPCs) via genetic engineering to overexpress Pim-1, a cardioprotec

274 This work lays the foundations for using genetic engineering to produce cellulose-based materials

275 to the environment and without the need for genetic engineering to safeguard feed and food supply fo

276 approaches, reprogramming patient cells and genetic engineering, to generate human induced pluripote

277 We present here the first to our knowledge genetic engineering tool that will efficiently allow for

278 It constitutes contribution to the genetic engineering toolkit of photosynthetic microorgan

279 The genetic engineering tools and resources established in t

280 y be applied to many other species for which genetic engineering tools are needed.

281 rganism that until recently lacked efficient genetic engineering tools.

282 RATIONALE: Targeted genetic engineering using programmable nucleases such as

283 Genetic engineering was undertaken to study the strength

284 In the study by Gorelik and colleagues (1), genetic engineering was used to overcome this limitation

285 This method, which does not require genetic engineering, was used to produce an anti-CD22 an

286 Using genetic engineering, we establish a link between the int

287 Using genetic engineering, we report that trimethylation of Ly

288 live-cell single-molecule tracking (SMT) and genetic engineering, we reveal that H3K27me3 contributes

289 nation of genomics, bioinformatics and mouse genetic engineering, we scanned approximately 700 kb of

290 single-molecule superresolution imaging and genetic engineering, we study in living Escherichia coli

291 regulatory strategy by chemical adjuvants or genetic engineering, which is restricted by limited syne

292 rative genomics, plant breeding, and plastid genetic engineering, while shedding further light on the

293 ir ability in stress sensitive crops through genetic engineering will be a meaningful approach to man

294 ending problems, significant improvements in genetic engineering will be needed to complement breedin

295 tated lignin recovery, and this coupled with genetic engineering will enable new uses for this biopol

296 tion in this work was the coupling of direct genetic engineering with a refolding approach to produce

297 s "super" immune cells, for example, through genetic engineering with chimeric antigen receptors.This

298 Here, by combining genetic engineering with quantitative super-resolution s

299 These developments promise genetic engineering with unprecedented levels of design

300 nted-by either chemical functionalization or genetic engineering-with hundreds to thousands of functi