ライフサイエンスコーパス: genetic screen

1 pe petioles was identified through a forward genetic screen.

2 erapeutic targets identified from functional genetic screens.

3 and present results from three complementary genetic screens.

4 mplex experimental designs and sophisticated genetic screens.

5 cterial gene expression coupled with forward genetic screens.

6 omplementary information in loss-of-function genetic screens.

7 cute myeloid leukaemia cells in two distinct genetic screens.

8 or use on data from large-scale CRISPR-based genetic screens.

9 ion for why these genes have evaded previous genetic screens.

10 neuromotor function in a Drosophila reverse genetics screen.

11 linical features and provide a rationale for genetic screening.

12 we can achieve high efficiency combinatorial genetic screening.

13 rmination of the nonclinical implications of genetic screening.

14 ambulatory ECG, an exercise stress test, and genetic screening.

15 thod has clear advantages over in silico and genetic screening.

16 a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identif

17 e this approach to perform an in vivo pooled genetic screen and identify Ptpn2 as a negative regulato

18 Through a sensitized genetic screen and molecular studies, we identified and

19 n in food analysis, forensic investigations, genetic screening and biodiagnostics.

20 ice increasingly relies on advanced imaging, genetic screening and devices.

21 igh-risk populations that would benefit from genetic screening and enhanced surveillance.

22 undant collection that is suitable for rapid genetic screening and gene discovery.

23 High-throughput genetic screening and gene expression profiling of fcp1

24 mutations, offering a promising approach for genetic screening and testing of LHON mutations.

25 ion of RNAi and CRISPR-Cas9 loss-of-function genetic screens and a small-molecule screen, we found th

26 We recently used CRISPRi/a-based chemical-genetic screens and cell biological, biochemical, and st

27 This proved a useful assay for genetic screens and enabled the identification of many c

28 ich to investigate how genes identified from genetic screens and genomic surveys function in choanofl

29 cts of phytohormones have been elucidated by genetic screens and hypothesis-driven approaches, and ex

30 address this, we performed CRISPR-Cas9-based genetic screens and identified cholesterol biosynthesis

31 Here, we use genetic screens and in vivo imaging in single neurons of

32 Genome-wide genetic screens and lipidomics revealed the dynamics of

33 accuracy of cancer gene discovery in forward genetic screens and provide initial insight into the bio

34 cell types; they are thus highly amenable to genetic screens and to defining molecular connectivity p

35 Here, using a combination of CRISPR-based genetic screens and unbiased lipidomics, we identified c

36 M development with a Drosophila melanogaster genetic screen, and identify Rab11b as a functional medi

37 i-lineage development, pan-tissue functional genetic screening, and tissue engineering.

38 e sequencing, transposon mutagenesis forward genetic screening, and transcriptomics.

39 ion, epigenetic engineering, genome imaging, genetic screens, and chromatin immunoprecipitation.

40 otypic patterns may increase productivity in genetic screens, and facilitate the study of genetic var

41 o gemcitabine, and a genome-wide CRISPR/Cas9 genetic screening approach identified only the pyrimidin

42 A simple, rapid, and flexible genetic screening approach identifies genes that drive r

43 In summary, our comprehensive functional genetic screening approach revealed modulation of resist

44 We employed a genetic screening approach to identify gain-of-function

45 We applied a scalable genetic screening approach, in vivo Perturb-Seq, to func

46 Here, we present a network-based genetic screening approach: the transcriptional regulato

47 Genetic screens are critical for the systematic identifi

48 Genetic screens are powerful tools for the functional an

49 Forward genetic screens are powerful tools for the unbiased disc

50 Genetic screens are powerful tools to dissect complex bi

51 Here, we designed a genetic screen around E. coli that identified high-affin

52 antages of CRISPRi-seq over transposon-based genetic screens, as all genes, including essential genes

53 to axis formation, I conducted a large-scale genetic screen at the beginning of my independent career

54 We previously established a forward genetic screen based on a virus-inducible green fluoresc

55 Here, by using forward genetic screens based on Ca(2+) imaging, we isolated hyd

56 in activity, we performed a forward chemical-genetics screen based on translocation of a glucose tran

57 reby increasing the sensitivity and scale of genetic screens by orders of magnitude.

58 Single-cell genetic screens can be incredibly powerful, but current

59 We hypothesized that a metabolism-restricted genetic screen, comparing normal primary mouse hematopoi

60 single-cell trajectory analysis with pooled genetic screening could reveal the genetic architecture

61 Here, we present a genetic screen coupled to pulse-chase labeling that allo

62 onse to butyrate by identifying mutants in a genetic screen defective for butyrate-modulated transcri

63 This study combines CRISPR-Cas9 genetic screens, degron assays, Hi-C, and cryoelectron m

64 n vitro biochemical studies and a cell-based genetic screen demonstrated that the interaction is spec

65 Previously, forward and reverse genetic screens demonstrated a requirement for pentatric

66 mutations in Nardilysin (dNrd1) in a forward genetic screen designed to isolate genes whose loss caus

67 ation-sensitive PCR (CHOP-PCR)-based forward genetic screen for Arabidopsis DNA hyper-methylation mut

68 ic causes of atopy, we carried out a forward genetic screen for atopy in mice.

69 By deploying an RNAi-based genetic screen for bak1/serk4 cell death suppressors, we

70 A genetic screen for C. elegans mutations that cause the s

71 ensing receptors, we carried out an unbiased genetic screen for cold-sensing mutants in C. elegans an

72 gevity control genes, we performed a forward genetic screen for delayed reproductive aging in C. eleg

73 on of ARK1 (Armadillo-Repeat Kinesin1) via a genetic screen for enhancers of the rhd3 mutant phenotyp

74 (EMC) was identified over a decade ago in a genetic screen for ER protein homeostasis.

75 induction of ER stress in yeast to enable a genetic screen for factors that augment stress resistanc

76 , and we exploit this phenotype to conduct a genetic screen for genes required for the homeostatic re

77 We identified IRF2 in a CRISPR-based forward genetic screen for genes that controlled MHC-I Ag presen

78 proteostasis, we performed a tissue targeted genetic screen for germline modifiers of polyglutamine a

79 We performed a forward genetic screen for imperial pils (imp) mutants that enha

80 A genetic screen for mutants defective for GAL gene memory

81 In this study, we conducted a genetic screen for mutants with altered leaf transpirati

82 From a genetic screen for mutations affecting early-endosome di

83 eterochromatin formation system to perform a genetic screen for mutations that abolish heterochromati

84 Here, we performed a forward genetic screen for mutations that de-repress Pho1 acid p

85 In an ongoing forward genetic screen for N-ethyl-N-nitrosourea (ENU)-induced m

86 A genetic screen for negative regulators of Mis4 yielded a

87 Furthermore, a genetic screen for NTZ-resistant bacterial mutants isola

88 In a genetic screen for pans1 suppressors, we identified SEPA

89 In a genetic screen for picloram resistance, we identified th

90 In a genetic screen for rapamycin-sensitive mutations, we iso

91 In the course of a forward genetic screen for recessive mouse mutants, we identifie

92 We performed a genetic screen for regulators of RAN translation and ide

93 m therapeutic entry points, we carried out a genetic screen for secondary mutations that improved phe

94 In a genetic screen for Staphylococcus aureus secreted virule

95 From a forward genetic screen for suppressors of heat-shock-induced gen

96 In a genetic screen for suppressors of the ibm2 mutation, we

97 nd-P metabolism is defective, we developed a genetic screen for synthetic interactions which, in comb

98 Here, via a forward genetic screen for TAG homeostasis, we isolated a Chlamy

99 Forward genetic screens for auxin-related mutants have led to th

100 Direct comparison to genetic screens for homologous recombination or Fanconi

101 orm and report a set of quantitative forward genetic screens for identifying regulatory mechanisms of

102 ese data demonstrate high-throughput in vivo genetic screens for immunotherapy target discovery and e

103 s associated with risk of AD, we performed a genetic screen from deep whole genome sequencing of the

104 e demonstrate its applicability in a forward genetic screen geared toward the identification of genet

105 A genetic screen has identified the first signaling compon

106 Genetic screening has identified numerous variants of th

107 CRISPR-based genetic screening has revolutionized cancer drug target

108 Large-scale forward genetic screens have been instrumental for identifying g

109 cells grow in a highly polarized manner, and genetic screens have identified many protein kinases, in

110 n fission yeast (Schizosaccharomyces pombe), genetic screens have previously uncovered a family of th

111 bditis elegans In this organism, even though genetic screens have uncovered 21U RNA biogenesis factor

112 Using genome-wide haploid genetic screens, here we identify the lipid-modifying en

113 screening; 8,136 (4.508%) were positive for genetic screening (heterozygote, homozygote, or compound

114 A Ca(2+)-imaging-based forward genetic screen identified a loss-of-function bon1 mutant

115 A forward genetic screen identified a mutation in the previously u

116 A genetic screen identified c3g as necessary for nephrocyt

117 Finally, a CRISPR-based genetic screen identified cholesterol uptake by the low-

118 In support of this, a forward genetic screen identified mutations in hesx1, cct5 and g

119 A forward genetic screen identified mutations in met-1, which enco

120 A genetic screen identified mutations that promote NE inte

121 This genetic screen identified the novel genetic interaction

122 Interestingly, a previously published genetic screen identified the T. brucei MCM-BP, which in

123 A genome-wide haploid genetic screen identified the transmembrane protein neur

124 Subsequent functional genetic screening identified host factors that functiona

125 Here, a forward genetic screen identifies a two-component system (KinB-A

126 A genetic screen identifies the phosphatase PPM1F as the c

127 In vivo genetic screening identifies CCL2 as the top prometastat

128 Reverse yeast genetic screens identify critical amino acid exchanges t

129 scovered approximately 20 years ago by yeast genetic screens identifying it as a factor responsible f

130 We performed a genome-scale forward genetic screen in a Drosophila model of tauopathy, a cla

131 we conducted a genome-wide loss-of-function genetic screen in an isogenic pair of human colorectal c

132 atical modeling, we also performed a forward genetic screen in Arabidopsis and discovered two mutants

133 We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CO

134 We have developed a genetic screen in Aspergillus nidulans for negative regu

135 From a genetic screen in C. elegans, we found that pix-1 is req

136 ors of BMP signaling, we performed a forward genetic screen in Caenorhabditis elegans for genes invol

137 In a forward genetic screen in Caenorhabditis elegans, we uncover tha

138 Here we use a genetic screen in Drosophila melanogaster to identify Ho

139 Here, we performed a genetic screen in Escherichia coli on the LCFA, oleate,

140 nt initiation process, we have carried out a genetic screen in fission yeast by random mutation of th

141 ling mechanism, we carried out a large-scale genetic screen in fission yeast looking for mutants with

142 ein stability and turnover, we established a genetic screen in mammalian cells by combining a fluores

143 Here, we use an unbiased genome-wide genetic screen in near-haploid human cells to uncover ce

144 , comprehensive, genome-wide CRISPR knockout genetic screen in PARP14-deficient cells.

145 We performed a genetic screen in Saccharomyces cerevisiae to identify h

146 By utilizing eroGFP in a comprehensive genetic screen in Saccharomyces cerevisiae, we show that

147 lator of renal cystic disease from a forward genetic screen in the mouse.

148 An in vivo genetic screen in the murine host identifies novel and k

149 rstood, particularly in vivo Using a forward genetic screen in the nematode Caenorhabditis elegans, w

150 PRMT5 inhibition, we performed a CRISPR/Cas9 genetic screen in the presence of a PRMT5 inhibitor.

151 Here, we performed a visual forward genetic screen in the zebrafish intestine and identified

152 Using a reverse genetic screen in yeast, we identify Cue2 as the conserv

153 Through a genetic screen in zebrafish and complementary analyses i

154 Through a genetic screen in zebrafish, we identified a mutant with

155 From a forward genetic screen in zebrafish, we identify the transcripti

156 aChA), which is a novel platform for haploid genetic screening in animals to identify genes essential

157 ISPR-Cas12a-based approach for combinatorial genetic screening in cancer cells.

158 el autophagy factors and pathways by forward genetic screening in mammalian cells.

159 Our experience with the HNP suggests that genetic screening in patients could identify at-risk car

160 ted in databases, and one (A243G) found in a genetic screening in patients with diabetes.

161 ene-level investigation of these mechanisms, genetic screening in the axolotl requires an extensive c

162 Genetic screening in the budding yeast Saccharomyces cer

163 Concurrent hearing and genetic screening in the whole newborn population in Bei

164 omedical researchers to conduct CRISPR-based genetic screens in a pooled format.

165 study of structure-function analyses and for genetic screens in a tractable vertebrate system.

166 ostasis and lipid metabolism.High-throughput genetic screens in animals could benefit from an easy wa

167 ential to accelerate the pace and utility of genetic screens in Arabidopsis.

168 From genetic screens in C. elegans, we identified splicing fa

169 e NECAP PHear domain that are predicted from genetic screens in C. elegans.

170 lying rapid nematode paralysis, we conducted genetic screens in Caenorhabditis elegans and isolated m

171 occus (enAsCas12a) for pooled, combinatorial genetic screens in human cells.

172 PR interference (CRISPRi)-based platform for genetic screens in human neurons derived from induced pl

173 We also discuss examples of CRISPR genetic screens in investigative dermatology and how the

174 Our results highlight the power of unbiased genetic screens in iPSC-derived differentiated cell type

175 Forward genetic screens in mammalian cell lines, such as RNAi an

176 Genetic screens in mammalian cells commonly focus on los

177 , we introduce a pooled approach for optical genetic screens in mammalian cells.

178 report the results of the first genome-wide genetic screens in the CNS using both short hairpin RNA

179 Genetic screens in the model alga Chlamydomonas have dem

180 perform unbiased RNAi and CRISPR-Cas9-based genetic screens in vivo.

181 Unbiased in vivo genome-wide genetic screening is a powerful approach to elucidate ne

182 , for example, by using computer testing and genetic screening, is an area of ongoing research.

183 Here, we circumvent aforementioned genetic screening limitations and present methods for a

184 est that other essential genes identified by genetic screens may turn out to be components of selfish

185 ighlights the utility of our straightforward genetic screening method in identifying new drug combina

186 Here we present a genetic screening method using photo-highlighting for ca

187 Recently, using a high-throughput genetic screen of a human interferon-stimulated gene sho

188 to GluA2(Q607E), a mutation observed in the genetic screen of a patient exhibiting developmental del

189 terized factor identified in our genome-wide genetic screen of CME.

190 In a forward genetic screen of mice with N-ethyl-N-nitrosourea-induce

191 In a forward genetic screen of N-ethyl-N-nitrosourea (ENU)-induced mu

192 Using a CRISPR-Cas9-based genetic screen of primary T cells we measured the multi-

193 We found Ddi1 in a genetic screen of the tdp1 wss1 mutant defective in Top1

194 w findings are informative and encourage the genetic screening of cancer patients in order to identif

195 germ cell manipulation, artificial gametes, genetic screening of embryos and gene editing of embryos

196 Chemical genetic screening of endothelial tube formation provides

197 ions and FTD/ALS syndromes and indicate that genetic screening of FTD/ALS patients for HTT repeat exp

198 An alternative to population-wide genetic screening of healthy individuals would exploit t

199 ethodology provides an accurate approach for genetic screening of imprinting related disorders in new

200 By a loss-of-function genetic screening of individual IFN-stimulated genes (IS

201 Concurrent hearing and genetic screening of newborns is expected to play import

202 aintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arab

203 Predictive genetic screening of relatives of patients with hypertro

204 Therefore, genetic screening of SCN9A, SCN10A and SCN11A should be

205 ses a MELAS-like phenotype, and suggests the genetic screening of the MRM2 gene in patients with a m.

206 a long-term monitoring program, we performed genetic screening of thousands of non-invasive samples c

207 Further genetic screening of unrelated PCD subjects identified a

208 w that the strongest epistatic pairings from genetic screens of three proteins, a ribozyme and a prot

209 Here, a chemical genetics screen of kinase inhibitors revealed phosphoino

210 ion, we performed a high-throughput chemical genetics screen of known inhibitors and agonists of host

211 Genetic screens on the sex determination genes in geneti

212 In an unbiased genetic screen, partial loss of Lilliputian (Lilli) acti

213 We discuss how each genetic screening platform can provide unique insight in

214 Forward genetic screens play a key role in the identification of

215 Our re-analysis of a previous genetic screening result in Caenorhabditis elegans shows

216 lowed by quantitative proteomic analysis and genetic screening revealed multiple regulators of N-medi

217 Surprisingly, genetic screening reveals that yeast FTase can modify se

218 Systematic, wide-ranging genetic screening should be offered in pES; the genetic

219 development of study models or as a tool in genetic screening studies, including those aiming to dis

220 Therefore, results from a chemical genetics screen suggest that intersections of the MAP ki

221 Forward genetic screening suggests that multiple receptors are i

222 ion of T cell targets, we developed a hybrid genetic screening system where the Sleeping Beauty (SB)

223 of liquid-liquid phase separation through a genetic screen targeting factors required for Arabidopsi

224 A genetic screen targeting regulators of mitochondrial dyn

225 cted a CRISPR-Cas9-mediated loss-of-function genetic screen that identified two epigenetic regulators

226 viable imb-2 allele from an unbiased forward genetic screen that reveals a specific role of imb-2 in

227 We have performed a behavioral genetic screen through the downregulation of candidate i

228 nstrate, to our knowledge, the first forward genetic screen to assign essential cellular functions to

229 Here, we design a forward genetic screen to discover factors in the postsynaptic c

230 We performed a Caenorhabditis elegans genetic screen to find cellular factors that affect the

231 d on a two-step Sleeping Beauty (SB) forward genetic screen to identify and validate new tumor suppre

232 egation with a CRISPR interference (CRISPRi) genetic screen to identify components of the endosomal s

233 Here, we used a chemical genetic screen to identify endothelial-specific direct s

234 We performed a genetic screen to identify factors responsible for gluco

235 Here, we perform the first genetic screen to identify genes required for hyphal gro

236 n a previous study, we established a forward genetic screen to identify genes required for multicellu

237 bining metabolic profiling with a Drosophila genetic screen to identify genetic modifiers of FXTAS, w

238 n this study, we performed a high-throughput genetic screen to identify kinases that enable tumor for

239 Using an unbiased genome-wide genetic screen to identify mutations affecting an FTD-AL

240 re, we report an antiviral necroptosis-based genetic screen to identify novel host cell factors requi

241 egress through CDPKs, we performed a forward genetic screen to isolate gain-of-function mutants from

242 Here we use a CRISPR genetic screen to uncover 140 Polq synthetic lethal (Pol

243 tion of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pherom

244 he new conserved CA proteins will facilitate genetic screening to identify patients with a form of pr

245 ons can be implemented in massively parallel genetic screens to evaluate the functional consequences

246 they can be used for high-throughput reverse genetic screens to help functionally annotate the Ae. ae

247 ng an important consideration when employing genetic screens to identify and characterize potential d

248 ying mechanism of this process, we performed genetic screens to identify components of the sorting ma

249 Here we describe a panel of genetic screens to identify genes required for replicati

250 series of gain- and loss-of-function forward genetic screens to identify modulators of resistance to

251 shed genetic and developmental model used in genetic screens to uncover genes necessary for early dev

252 s the use of iterative "perturb and observe" genetic screens to uncover regulatory mechanisms driving

253 Here, we have used forward chemical genetics screening to identify DFPM-insensitive loci by

254 Using an unbiased genetic screen, To et al.

255 Using an unbiased genetic screen, To et al. map genes that enhance or supp

256 Forward genetic screen, typically performed in invertebrates or

257 Genetic screening used a combination of single-gene test

258 hanges in FXTAS pathogenesis, we performed a genetic screen using a Drosophila model of FXTAS.

259 We then performed an unbiased genetic screen using a Drosophila model of the G4C2 repe

260 we performed an shRNA-based loss-of-function genetic screen using a kinome library.

261 Finally, we performed a genetic screen using RN4, the RN with the greatest poten

262 Here, from a forward genetic screen using zebrafish, we report the discovery

263 creening Applications (CHyMErA), outperforms genetic screens using Cas9 or Cas12a editing alone.

264 Genetic screens using high-throughput fluorescent micros

265 Genetic screens using pooled CRISPR-based approaches are

266 re we develop a multidimensional CRISPR-Cas9 genetic screen, using multiple mitophagy reporter system

267 d as a LD marker in C. elegans and a forward genetic screen was carried out to find novel LD regulato

268 On the basis of a directed genetic screen we find that vision plays a key role in c

269 Through a forward genetic screen we found pals-22, a gene of previously un

270 Through forward genetic screens we identified PKL, a gene required for d

271 In a genetic screen, we discovered a large number of endolyso

272 In a genetic screen, we found a zebrafish strain in which mit

273 Using a genetic screen, we found that CALCINEURIN B-LIKE INTERAC

274 Based on an unbiased genetic screen, we found that partial genetic and pharma

275 directed mutagenesis and an unbiased forward genetic screen, we have identified a group of highly con

276 Using a forward genetic screen, we identified a homolog of eptA, a predi

277 Using a forward genetic screen, we identified a mutation in a receptor-b

278 Drosophila as a model system in an unbiased genetic screen, we identified a number of genetic modifi

279 Using a forward genetic screen, we identified an allelic series of GmSHM

280 Using a genetic screen, we identified EMI1 as a modulator of PAR

281 Using a chemical genetic screen, we identified the Hsp70 chaperone family

282 of zebrafish mutants isolated in an unbiased genetic screen, we identified the palmitoyltransferase H

283 Using a zebrafish genetic screen, we identified the ric1 gene as being ess

284 Through a forward genetic screen, we identify mutants that exhibit dramati

285 Here, by a forward genetic screen, we identify two dominant mutations of th

286 By coupling in vivo ribosome profiling with genetic screening, we provide direct evidence that oncog

287 Using forward genetic screens, we find that dendrite development requi

288 Through genetic screens, we identify mutations that restore defe

289 Here, using Ca(2+)-imaging-based forward genetic screens, we isolated the Arabidopsis thaliana mu

290 Using genetic screens, we show that lack of the checkpoint-blo

291 Through forward genetic screens, we then determined that autophagosome m

292 48, where the phenotype predictions from the genetic screen were fulfilled in our studies of transgen

293 e LL5 and the V5-like myophage LL12, forward genetic screens were conducted against the Keio collecti

294 , autoantibody panel, infectious etiologies, genetic screening, whole exome sequencing, and the phage

295 transmission, we conducted a high-throughput genetic screen with a transposon sequencing (Tn-seq) lib

296 We performed a genetic screen with ag-10 plants, which exhibit a weak f

297 A forward genetic screen with N-ethyl-N-nitrosourea (ENU)-mutageni

298 allel pooled genome-wide CRISPR-Cas9 forward genetic screening with a highly quantitative and sensiti

299 ions constrain sensitivity and throughput of genetic screening with single-cell transcriptomics reado

300 Here we identify via a CRISPR-Cas9 genetic screen ZNF410, a pentadactyl DNA-binding protein