ライフサイエンスコーパス: genetic system

1 for studying tendon biology in a vertebrate genetic system.

2 exemplify primitive states of the developing genetic system.

3 erproduce SHI utilizing a recently developed genetic system.

4 hampered by the lack of a tenable chlamydial genetic system.

5 tly to accelerate the use of S. viridis as a genetic system.

6 acilitating rapid transfer to any compatible genetic system.

7 related to the underlying properties of the genetic system.

8 ymes can serve as an experimental model of a genetic system.

9 it is one of few archaea with an established genetic system.

10 hicken cells to dissect its role in a stable genetic system.

11 red by IFN-inhibited PKR function in a yeast genetic system.

12 to the proper implementation of this complex genetic system.

13 incorporation of phosphate and an RNA-based genetic system.

14 e switching) embedded in a uniquely powerful genetic system.

15 e easily adaptable to circuit mapping in any genetic system.

16 ally interact and function together in other genetic systems.

17 on of the relative complexities of different genetic systems.

18 are assembled from subunits derived from two genetic systems.

19 n and investigate its consequences for model genetic systems.

20 organism-based research and the evolution of genetic systems.

21 troses) that may also be able to function in genetic systems.

22 ant bacterial pathogens with no or difficult genetic systems.

23 cal processes because of a lack of tractable genetic systems.

24 ruction and systematic optimization of large genetic systems.

25 bility of genome sequence data and molecular genetic systems.

26 ays and the evolutionary dynamics of complex genetic systems.

27 d, yet little is known about how they modify genetic systems.

28 typing individual sites, proteins, or entire genetic systems.

29 ractive complementary technology to existing genetic systems.

30 er of genetic information between successive genetic systems.

31 with replication stress as known from other genetic systems.

32 otein expression is critical when optimizing genetic systems.

33 may be a common force in standard population genetic systems.

34 tructing, and characterizing such artificial genetic systems.

35 rgetic organelles with their own genomes and genetic systems.

36 nked by shared, underlying physiological and genetic systems.

37 rtificial chromosome (BAC)-based MHV reverse genetics system.

38 virus, were generated by use of the reverse genetics system.

39 h facilitate recovery of MNV using a reverse-genetics system.

40 he virus S and L segments by using a reverse genetics system.

41 antage of a modified fully tractable reverse-genetics system.

42 acid mutation at this site through a reverse genetics system.

43 with an antibody avidity assay based on the Genetic Systems 1/2+ O ELISA.

44 How two-component genetic systems accumulate evolutionary novelty and dive

45 y heritable epigenetic information represent genetic systems adjunct to DNA sequences that contribute

46 ized biological parts in the construction of genetic systems aimed at eliciting of desired cellular b

47 Phage genetic systems already support the perspective that lys

48 .3 strain U201 RNA, generated from a reverse genetics system, also does not induce an IFN response.

49 Here we study a simple genetic system, an evolving RNA enzyme (ribozyme) in whi

50 rospective of Drosophila as an indispensable genetic system and discuss some of the many contribution

51 ism of aCFV, we used the geminiviral reverse genetic system and introduced a multimeric clone into th

52 cking the functions of the natural DNA-based genetic system and of developing useful research tools.

53 ubstitutions were made within a TBEV reverse genetic system and recovered mutants were compared for p

54 Collectively, the reverse genetic system and reporter virus provide key reagents t

55 should improve the engineering of synthetic genetic systems and also advance quantitative modeling o

56 research is relying on increasingly complex genetic systems and circuits to perform sophisticated op

57 n view of the future selection of artificial genetic systems and dedicated polymerases as well as app

58 of resource loading in engineered mammalian genetic systems and develop an endoribonuclease-based fe

59 They combine highly developed genetic systems and ease of use with reductions in time

60 information between organelles with distinct genetic systems and have established a new level of inte

61 Compared with genetic systems and other in vitro methods, IVT2H not on

62 Advanced recombinant genetic systems and the recent discovery of a mouse-trop

63 yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new s

64 derstanding evolutionary dynamics of complex genetic systems and unveiling structure and function of

65 By taking advantage of the available reverse genetics system and ectopic expression system for viral

66 In summary, the reverse-genetics system and minireplicon reporter assay describe

67 tions at these three residues with a reverse genetics system and then investigated the molecular basi

68 hat have been developed in such traditional 'genetic systems', and highlight the progress that has be

69 al tolerance mechanisms, implementation of a genetic system, and discovery of a new carbon fixation p

70 ries that unify many deeply diverged ciliate genetic systems, and a surprising evolutionary "sign cha

71 nctions of the ribosome, enabling orthogonal genetic systems, and engineering ribosomes with new func

72 f parenting on the child's physiological and genetic systems, and how interventions can improve child

73 manipulation of many organisms for which few genetic systems are available.

74 Expanded genetic systems are most likely to work with natural enz

75 Engineered genetic systems are prone to failure when their genetic

76 f Tn7-like elements broadens our view of how genetic systems are repurposed and provides potentially

77 ignature of coevolution of these two complex genetic systems as compared with inhibitory receptor-lig

78 There is no reverse genetics system available for the current epidemic virus

79 pore-forming bacterium and model prokaryotic genetic system, Bacillus subtilis, is extremely useful i

80 We used a synthetic genetic system based on ligand-induced intramembrane pro

81 A genetic system based on rpsL-streptomycin counter select

82 Here, we present a novel genetic system based on the chicken DT40 cell line, in w

83 and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico

84 Therefore, we developed a reverse genetics system based on the neurovirulent Snyder Hill (

85 virus (ZEBOV) glycoprotein (GP) by a reverse genetics system based on the SAD B19 RABV wildlife vacci

86 Studies in novel genetic systems, both at the base of the land plant phyl

87 Chlamydomonas was rooted in its promise as a genetic system, but like other classic model organisms,

88 ctionality of aptazymes in fully cytoplasmic genetic systems, but also implicates general feasibility

89 ing reovirus rescue from the current reverse genetics system by 100-fold.

90 Using a mouse genetic system called mosaic analysis with double marker

91 , in turn, implies that the GACTZP synthetic genetic system can explore the entire expanded sequence

92 A subset of these genetic systems can exchange information with RNA and DN

93 RV isolate, demonstrating that this reverse genetics system can be used to study the molecular biolo

94 This reverse genetics system can be used to study the molecular virol

95 We have developed a genetic system capable of producing modular carbon-fixin

96 In early vertebrates, a genetic system coding for ancient soft collagenous tissu

97 We analyzed various genetic systems comprising HIV-1, the malaria-causing pa

98 Both genetic systems contain two-gene operons that are transc

99 The genetic systems controlling body axis formation trace ba

100 Study of this unique genetic system coupled with proteomic analysis indicated

101 he complete genomic sequence and the reverse genetics system described herein make TV a valuable mode

102 The robust reverse genetics system described will be a valuable tool for th

103 ation and extended in a delayed fashion in a genetic system designed to preclude downstream repair st

104 Using the murine norovirus (MNV) reverse-genetics system, disruption of 5' end stem-loops produce

105 e that TNA could have served as an ancestral genetic system during an early stage of life.

106 To address these issues, a yeast-based genetic system enabling generation of stable strains exp

107 nity, Long and coworkers describe a powerful genetic system enabling reliable serial genetic dissecti

108 This highly efficient genetic system facilitates high-throughput functional ch

109 Here, we exploited a reverse genetic system for an enteric CoV, porcine epidemic diar

110 of other animals suggest that a pre-existing genetic system for anteroposterior head patterning was c

111 is manuscript, we describe a CRISPR-mediated genetic system for cells of the myeloid lineage in which

112 Since there is no current genetic system for Chlamydia spp., blockade of T3S was a

113 pressing transgenic plants may provide a new genetic system for dissecting the molecular mechanism th

114 Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrat

115 The development of a genetic system for making recombinant rhLCV constitutes

116 nistic information obtained in this powerful genetic system for mammalian mitosis.

117 We report a reverse genetic system for SARS-CoV-2.

118 Here, we report on the development of a genetic system for the detection of T(12;15)(Igh-Myc) tr

119 netic bases, and they establish an important genetic system for the study of early stages of ecologic

120 the family Y Pols, and the availability of a genetic system for this archaeon should allow subsequent

121 We developed a genetic system for tunable PC-specific coexpression of s

122 These new genes are the basis for a novel genetic system for various mineralized tissues in skelet

123 l advances in glycan binding assays, reverse genetic systems for influenza and in X-ray crystallograp

124 We constructed reverse genetic systems for JHM.SD and JHM.WU and, utilizing the

125 of this novelty is co-option of pre-existing genetic systems for new purposes.

126 By taking advantage of the available reverse genetics system for a model arenavirus, Pichinde virus (

127 ng DWV genotypes, thereby devising a reverse-genetics system for an invertebrate RNA virus quasispeci

128 We constructed a reverse genetics system for avian paramyxovirus serotype 7 (APMV

129 veloped the first, to our knowledge, reverse genetics system for HRV-C.

130 In this report, we describe a reverse genetics system for NL63, whereby five contiguous cDNAs

131 A newly described single-plasmid reverse genetics system for noroviruses has the potential to fac

132 is end, we developed a plasmid-based reverse genetics system for Orsay virus by creating transgenic C

133 s functional minigenome system and a reverse genetics system for production of infectious Junin virus

134 In this study, we constructed a reverse-genetics system for recovery of infectious APMV-3 strain

135 yxoviruses (APMVs), we constructed a reverse genetics system for recovery of infectious recombinant A

136 In this study, we constructed a reverse genetics system for recovery of infectious recombinant A

137 stablishment of a plasmid only-based reverse genetics system for rotaviruses by several Japanese rese

138 A reverse genetics system for the highly virulent NDV strain Banja

139 We generated a reverse genetics system for the live-attenuated MV vaccine strai

140 ing potential acceptor sites using a reverse genetics system for the moderately pathogenic strain Bea

141 A novel reverse genetics system for three representative DWV variants, i

142 investigations, we have developed a reverse-genetics system for UUKV that permits manipulation of th

143 facilitated by the establishment of reverse genetics systems for the genetic manipulation of the TCR

144 rovirus (MNV) and the development of reverse-genetics systems for this virus provide an opportunity t

145 The development of reverse genetics systems for wild-type strains of CDV and the us

146 Fragile X syndrome, the classical Drosophila genetic system has provided fruitful disease models.

147 gy approaches and the development of reverse genetics systems has allowed the rapid and reliable desi

148 The available data from uniparental genetic systems have already transformed our view of the

149 Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were muta

150 walk virus stool RNA transfection, a reverse genetics system, IFN neutralization reagents, and shRNA

151 embers of human APOBEC3 family using a novel genetic system in Escherichia coli.

152 complex (MHC) contains the most polymorphic genetic system in humans, the human leukocyte antigen (H

153 ssues, we have developed a novel conditional genetic system in mice based on the heterologous express

154 To study CytoQC, we have employed a genetic system in Saccharomyces cerevisiae using a trans

155 Here we describe a genetic system in the nematode Caenorhabditis elegans to

156 We have created a genetic system in the yeast Saccharomyces cerevisiae to

157 We describe here a chemical genetic system in which a single Plk family member, Plk1

158 editing in human cells to create a chemical genetic system in which endogenous Plk4 can be specifica

159 other enrichment program (MEP), an inducible genetic system in which mother cells maintain a normal R

160 Using a mouse genetic system in which restricted populations of neuron

161 Using a genetic system in yeast that allows detection of mitotic

162 ive that enables comprehensive evaluation of genetic systems in a single, readily-prepared transfecti

163 One of the most intriguing genetic systems in B. burgdorferi is vls recombination,

164 ough the analysis of uniparentally inherited genetic systems in Native American populations and the c

165 nisms that poorly represent the diversity of genetic systems in nature.

166 nsects to engineer female-specific autocidal genetic systems in the Mediterranean fruit fly, Ceratiti

167 his is the first plasmid-based virus reverse genetics system in the metazoan C. elegans.

168 we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mC

169 Through use of an MHV reverse genetics system, in-frame deletions of the coding sequen

170 enges the understanding of even the simplest genetic systems, including the prototypical lac operon.

171 ult myocardium using a doxycycline-inducible genetic system (inducible Notch intracellular domain [iN

172 By using a cDNA-based reverse genetics system, insertions or substitutions of sequence

173 ep in synthetic biology to use an artificial genetic system inside of living bacterial cells.

174 e provides a blueprint for understanding the genetic systems involved in alkane metabolism including

175 epigenetic regulation of important microbial genetic systems involved in health promotion, safety, an

176 A common topology found in many bistable genetic systems is two interacting positive feedback loo

177 The precision of this genetic system largely depends on the specificity of Cre

178 nt gaps in our conceptual framework of early genetic systems make it difficult to imagine how a stabl

179 ss the evolution of the unique mitochondrial genetic system, mitochondrial metabolites, mitonuclear c

180 gent to develop representations of synthetic genetic systems more abstract than their DNA sequence.

181 The aim of synthetic biology is to make genetic systems more amenable to engineering, which has

182 This study exploits the genetic system of Salmonella enterica to examine the in

183 ccus faecalis is the most thoroughly studied genetic system of this species.

184 Animal models combined with reverse genetic systems of anellovirus have not been developed.

185 e ancient events can significantly shape the genetic systems of the recipients and can play a part in

186 Using a reverse genetics system of a prototypic arenavirus, Pichinde vir

187 Using a reverse genetics system of a prototypic arenavirus, Pichinde vir

188 A reverse genetics system of PRRSV North American prototype VR-233

189 Insects carrying a female-specific autocidal genetic system offer an attractive alternative to conven

190 rdial soups" toward structure, function, and genetic systems over time.

191 This "pro-active" genetic system (Pro-AG) functionally inactivates an anti

192 However, due to the lack of genetic systems, protein transfer by the C. burnetii Dot

193 ion accelerates and simplifies the design of genetic systems, providing a new high-information strate

194 Utilizing a reverse-genetics system recently developed, we report the rescue

195 Using a reverse genetics system, recombinant hMPVs (rhMPVs) lacking eith

196 How divergent genetic systems regulate a common pathway during the dev

197 ing region; however, it remains elusive what genetic system regulates the Hand2-Shh pathway in the hi

198 The reliable forward engineering of genetic systems remains limited by the ad hoc reuse of m

199 new DNA folds that will emerge in synthetic genetic systems replacing standard DNA and RNA as platfo

200 oteobacteria, and it is likely that the VtlR genetic system represents a common regulatory circuit cr

201 Natural and engineered genetic systems require the coordinated expression of pr

202 lts of minigenome assays and an EBOV reverse genetic system rescue support a role for both the panhan

203 study IDV at the molecular level, a reverse-genetics system (RGS) is urgently needed, but to date, n

204 We propose a genetic system, Semele, which may be used for both.

205 Molecular analyses of these simple genetic systems show rapid evolution, positive selection

206 ficiency aspects of this Junin virus reverse genetics system show great promise for addressing import

207 Synthetic genetic systems so-encoded should fail more safely in re

208 This suggests the possibility that other genetic system structures besides the natural one might

209 n pathogens, including NTHI, possess a novel genetic system, termed the phasevarion, which mediates a

210 Here we report a Cre/loxP-based genetic system that allowed inducible deletion of specif

211 We previously developed a synthetic genetic system that allows a laboratory strain of Escher

212 We report here the use of a viral genetic system that allows for deletion of specific vira

213 A genetic system that contains more bits than the number t

214 Further, we developed a genetic system that enabled us for the first time to iso

215 nt genome-editing tools comprises a powerful genetic system that enables facile insertion and deletio

216 f yeast ribosomes were created using a yeast genetic system that enables stable production of ribosom

217 Here, we used a Saccharomyces cerevisiae genetic system that generates gross chromosomal rearrang

218 ve started with a heterogeneous nucleic acid genetic system that included both RNA and DNA(4).

219 However, a genetic system that permits tracing the early events of

220 In the genetic system that regulates complex traits, metabolite

221 ic approach to TBI in Drosophila, a powerful genetic system that shares many conserved genes and path

222 ts the development of specialized orthogonal genetic systems that could be evolved for novel function

223 they evolve to be critical components of the genetic systems that determine the biological diversity

224 owing that new genes rapidly change existing genetic systems that govern various molecular, cellular,

225 The genetic systems that initially suggested stress-induced

226 tors--are at the center of physiological and genetic systems that permitted the evolution of the huma

227 specificity of this response is conferred by genetic systems that restrict fusion to self and close k

228 ent after the development of the BTV reverse-genetics system that allows the introduction of targeted

229 enome replication assay and a robust reverse-genetics system that can be used to further study replic

230 e have developed a plasmid-based IDV reverse-genetics system that can generate infectious viruses wit

231 first established a highly efficient reverse genetics system that increased rescue titers by >/=3 log

232 inally, we outline the importance of reverse genetics systems that can swiftly characterize filovirus

233 Using reverse genetics systems that we have recently developed, we hav

234 In several genetic systems, the speed of adaptation has been attrib

235 Compared to the classical reverse genetics system, the "eight-in-one" bacmids (bcmd-RGFlu)

236 capitulates a central aspect of an RNA-based genetic system: the RNA-catalyzed synthesis of an active

237 nsmitter to regulate locomotion, providing a genetic system to analyze serotonin signaling.

238 ed in other studies, we have developed a new genetic system to characterize ffs mutants.

239 Thus, C. elegans may be used as a genetic system to characterize novel cellular and molecu

240 eagues have further exploited the Drosophila genetic system to characterize two new phototransduction

241 We have developed a genetic system to conditionally misexpress Tbx2 in the e

242 Here, we describe a genetic system to conditionally reduce ATR expression to

243 hat are infectious in cell culture provide a genetic system to evaluate the significance of virus-hos

244 established an NAD(+) intermediate-specific genetic system to identify factors that regulate the de

245 Here, we exploited the yeast genetic system to identify members of the mammalian AMPK

246 sophila adult midgut proves to be a powerful genetic system to identify novel mediators of APC phenot

247 model of tissue replacement that utilized a genetic system to identify transplanted cells and induce

248 In lieu of a functional genetic system to investigate floral development in thes

249 We have developed a genetic system to investigate the mechanisms of DNA doub

250 ation theory approach can be extended to any genetic system to predict new sites for DNA-binding prot

251 Dispensability provides an advantageous genetic system to probe the mechanisms of division prote

252 We have used a vaccinia virus-based reverse genetic system to produce recombinant viruses encoding n

253 Using a tripartite genetic system to selectively ablate either V1 or V2b IN

254 Using a chemical-genetic system to selectively and reversibly inhibit Zap

255 Herein, we deploy a genetic system to starve cells of an essential ribosomal

256 t stem cells may provide the necessary human genetic system to study germ cell development.

257 In an effort to develop a genetic system to study SifA function, we expressed SifA

258 Chlamydomonas reinhardtii provides a simple genetic system to study the role of the centriole in cil

259 pericarp and cob glumes, offers an excellent genetic system to study tissue-specific gene regulation.

260 In this study we used a genetic system to titrate the rate of GPCR signaling in

261 isms, we made use of the tet-On and Cre-loxP genetic systems to selectively and acutely express polyQ

262 We developed a reverse genetics system to create a mutant of RRV (RRV(VP4-R446G

263 We utilized a reverse genetics system to generate a GFP reporter virus to expl

264 ly efficient Rift Valley fever virus reverse genetics system to generate live recombinant viruses wit

265 dy, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions that corre

266 nfirmed using a vaccinia virus-based reverse genetics system to produce a recombinant virus, Bristol

267 We have developed a reverse genetics system to produce live recombinant PICV (strain

268 We established a reverse-genetics system to recover UUKV entirely from cDNA clone

269 cle (DISC) vaccine strain, we used a reverse genetics system to rescue defective virus strains with l

270 A reverse genetics system to rescue infectious Lujo virus from cDN

271 port the development of an efficient reverse genetics system to rescue recombinant LASV and to invest

272 tch in vitro, we first established a reverse genetics system to rescue UUKV with a genome close to th

273 Therefore, the use of reverse genetics systems to engineer viruses lacking NSs is an a

274 Here we report the development of reverse genetics systems to study STFSV replication and pathogen

275 This ZIKV reverse genetic system, together with mouse and mosquito infecti

276 A genetic system utilizing alkaline phosphatase secretion

277 In this study, a genetic system was developed for M. sedula to investigat

278 A genetic system was developed for MLHE-1 and used to show

279 nt lines of evidence suggest that the modern genetic system was preceded by the 'RNA world' in which

280 Recently, a highly efficient reverse genetics system was developed that allows genetic manipu

281 The reverse genetics system was used to analyze the role of the clea

282 Moreover, the reverse genetics system we constructed will be helpful for study

283 king dendrite structure and function in this genetic system, we analyzed da neuron axon projections i

284 Using a novel genetic system, we demonstrated that seven of the nine g

285 nces of G4C2 repeat expansion in a tractable genetic system, we generated transgenic fly lines expres

286 Using the Pichinde virus (PICV) reverse genetics system, we analyzed the effects of alanine subs

287 Using an IBV reverse genetics system, we demonstrated that the template-switc

288 d by organisations in DR Congo and a reverse genetics system, we generated an authentic Ebola virus f

289 Using the SARS-CoV reverse genetics system, we generated and characterized a chimer

290 Using a reverse genetics system, we generated recombinant RSV (rRSV) con

291 By using the reverse-genetics system, we identified that 4 amino acid substit

292 Using the eight-plasmid reverse genetics system, we rescued wild-type SIV A/swine/Minnes

293 Using an RV reverse-genetics system, we show that compared to the parental s

294 Using the reverse genetics system, we show that it is possible to modify o

295 ICV) GP expression vector and a PICV reverse genetics system, we systematically characterized the fun

296 ults indicate that the BAC-based MHV reverse genetics system will be useful for studies of JHMV and e

297 This optimized henipavirus reverse genetics system will facilitate future investigations in

298 This improved RV reverse genetics system will facilitate study of RV biology in b

299 llespie's results are extended to population genetic systems with explicit age structure, where the d

300 ted substitutions is an emergent property of genetic systems with implications for evolution, vaccine