ライフサイエンスコーパス: genome editing

1 many applications including transgenesis and genome editing.

2 age immune system that can be repurposed for genome editing.

3 uilt on recent advances in CRISPR/Cas9-based genome editing.

4 ty essential for both bacterial immunity and genome editing.

5 very of the CRISPR-Cas machinery for in vivo genome editing.

6 to cell lines to enable CRISPR-Cas9-mediated genome editing.

7 precludes safe and reliable applications in genome editing.

8 on with other technologies, such as RNAi and genome editing.

9 of the most recent advances in the field of genome editing.

10 is one of the most frequently used tools for genome editing.

11 or C*G to T*A, in cellular DNA for precision genome editing.

12 o be harnessed as programmable nucleases for genome editing.

13 he CRISPR-Cas9 system is a powerful tool for genome editing.

14 development of safe and efficacious clinical genome editing.

15 pand the application of CRISPR/Cas9-mediated genome editing.

16 ems provide versatile tools for programmable genome editing.

17 ins in cells using small interfering RNAs or genome editing.

18 periments and guide RNAs for CRISPR-mediated genome editing.

19 reat potential to enable a more controllable genome editing.

20 ir development as highly effective tools for genome editing.

21 ed topics related to genetic engineering and genome editing.

22 SNF genetics in the era of pan-genomics and genome editing.

23 cal TFH genes BCL6 and CXCR5 as confirmed by genome editing.

24 ontrol of the CRISPR/Cas9 system for precise genome editing.

25 he mechanistic basis of CRISPR-Cas9-directed genome editing.

26 ch expands the molecular biology toolkit for genome editing.

27 re directed towards retinal reprogramming or genome editing.

28 for transient Cas9 expression and efficient genome editing.

29 nding the arsenal of enzymes used in precise genome editing.

30 and tRNA-mediated or Csy4-mediated multiplex genome editing.

31 ompact enough to be packaged into an AAV for genome editing.

32 s for the proper use of CRISPR-Cas9-mediated genome editing.

33 on the efficiency of these PAM sequences in genome editing.

34 to control mammalian gene expression and for genome editing.

35 ed a simple yet powerful system for targeted genome editing.

36 As to genome editing, a 3-fold repression in the EGFP expressi

37 -specific promoters, the Cre/lox system, and genome editing, AAVs represent a practical, rapid, and e

38 and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observe

39 have explored many approaches to improve the genome editing activity of the CRISPR-Cas system and del

40 ased efficiency variant (iSpyMac) for robust genome editing activity.

41 tal cancer (CRC) cells, thus displaying high genome-editing activity in vitro.

42 We analyze key considerations when choosing genome editing agents and identify opportunities for fut

43 Four classes of CRISPR-Cas-derived genome editing agents-nucleases, base editors, transposa

44 in providing a safe approach for therapeutic genome editing, agriculture, and other applications.

45 nventional crossing and induced mutagenesis, genome editing aims to improve crop yield and nutrition.

46 Rapid progress in the use of genome editing also is paving the way for the treatment

47 However, the efficiency of genome editing also largely depends on the endogenous ce

48 site interactions, ecotoxicology, evolution, genome editing and 'omics', and human disease modelling.

49 olting and to identify potential targets for genome editing and breeding.

50 is Perspective, we discuss advances in human genome editing and consider ethical questions and potent

51 Useful for genome editing and DNA detection but with a molecular we

52 RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection.

53 To extend the frontier of genome editing and enable editing of repetitive elements

54 en harnessed for the development of powerful genome editing and engineering tools.

55 synthetic methylotrophic strain illustrates genome editing and evolution for microbial tropism chang

56 ificantly improve the accuracy of Cpf1-based genome editing and facilitates the generation of optimiz

57 We also combined GRF4-GIF1 with CRISPR-Cas9 genome editing and generated 30 edited wheat plants with

58 otemporal dementia patient using CRISPR/Cas9 genome editing and homology-directed repair (HDR), resul

59 ptual advances, such as the establishment of genome editing and improved phylogenetic resolution, are

60 yogenes was the first Cas9 nuclease used for genome editing and it remains the most popular enzyme of

61 Using CRISPR/Cas9 genome editing and lentiviral transduction, we generated

62 discuss the recent applications of tRNAs in genome editing and microbiome sequencing.

63 ells execute this process, we combine CRISPR genome editing and MS2 RNA tagging to image single molec

64 ring the activity of engineered nucleases in genome editing and other biotechnological applications a

65 csk9 in adult mouse liver produces efficient genome editing and reduced serum cholesterol with except

66 lication of TALE- or TALEN-based systems for genome editing and regulation.

67 eadouts from functional experiments, such as genome editing and reporter assays.

68 Here, using genome editing and RNA interference, we examine transcri

69 iscuss the DNA repair pathways that underlie genome editing and strategies to favour various outcomes

70 n with biotechnological innovations, such as genome editing and surrogate broodstock technologies, ma

71 R-Cas proteins have been widely developed as genome editing and transcriptional regulating tools.

72 CRISPR-Cas systems are now widely used for genome editing and transcriptional regulation in diverse

73 acterial RNA-guided nuclease used widely for genome editing and, more recently, as a molecular diagno

74 letion extends dCas9 residence times, delays genome editing, and alters the balance between indel for

75 simple NNGG PAM, displays high activity for genome editing, and is compact enough to be packaged int

76 of marker-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for genet

77 cantly increases the efficiency of precision genome editing, and such a platform is compatible with s

78 mophilic Type II-C enzyme that has potential genome editing applications in extreme environments.

79 ld facilitate the manipulation of Cas12i for genome editing applications.

80 utility of high-resolution targeting across genome editing applications.

81 Cas9, with potential advantages for specific genome editing applications.

82 In addition to providing examples of genome-editing applications in crop breeding, we discuss

83 efSaCas9 can be broadly used in genome-editing applications that require high fidelity.

84 Here, we used a "cloning-free" saturation genome editing approach in a diploid cell line to simult

85 y, we demonstrate the feasibility of using a genome-editing approach to generate anephrogenic foetuse

86 Anticipating and verifying the result of genome editing are essential for the success for all app

87 atforms, protocols and vectors for precision genome editing are now available, leading to the develop

88 -directed nucleases (SDNs) used for targeted genome editing are powerful new tools to introduce preci

89 target effects; all are essential for moving genome editing based SCD treatment into clinical practic

90 Gaa gene, this model is also not amenable to genome-editing based therapeutic approaches.

91 ting, presenting a bottleneck in therapeutic genome editing, because even a small number of cells wit

92 observe robust inhibition of SauCas9-induced genome editing by AcrIIA13 and moderate inhibition by Ac

93 d provide new insights into the mechanism of genome editing by Cas9.

94 Genome editing by CRISPR (clustered regularly interspace

95 Thus, we enable precise genome editing by establishing a flexible approach for s

96 iral vectors (MNP-BVs), CRISPR-Cas9-mediated genome editing can be activated locally in vivo via a ma

97 s remain before the therapeutic potential of genome editing can be fully realized.

98 ced short palindromic repeats (CRISPR)-based genome editing can be limited by a lack of compatible pr

99 ewly engineered SpRY will potentially expand genome-editing capabilities for basic and applied resear

100 l engineering efforts aimed at improving its genome-editing capability.

101 ected repair rates, and very low nonspecific genome editing compared to SpCas9.

102 Introduction of this minor allele SNP by genome editing confirmed its functionality in depressing

103 Precise genome editing/correction of DNA double-strand breaks (D

104 Using a combination of genome editing (CRISPR-Cas9), electrophysiological recor

105 gh base editors are useful tools for precise genome editing, current base editors can only convert ei

106 RNA binding experiments together with genome editing demonstrate that FOX-1 binds to multiple

107 R-Cas defense systems opened up the field of genome editing due to the ease with which effector Cas n

108 e the resolution of molecular genetics, make genome editing easier, and may be useful in regenerative

109 esenting a significant improvement in citrus genome editing efficacy.

110 for citrus that significantly improve citrus genome editing efficacy.

111 The superior drug delivery/gene transfection/genome-editing efficiencies of the SMOF NP are attribute

112 nuclease for its cognate PAM promotes higher genome-editing efficiency.

113 science is published and new techniques like genome editing emerge, reanalysis of some of these issue

114 In addition, the genome editing enzymes themselves need to be optimized,

115 olecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasPhi offers advantages for cel

116 quantification and visualization of genuine genome editing events.

117 gens is to use genetic modification (GM) and genome editing, expanding the breeder's toolkit.

118 leases is an important consideration for any genome editing experiment, and a number of Cas9 variants

119 ated (Cas) nucleases have revolutionized the genome editing field.

120 a combination of cell-based reporter assays, genome editing, flow cytometry, and immunofluorescence m

121 Genome editing followed by reproductive cloning was prev

122 table strategy to improve the specificity of genome editing for most genomic loci.

123 a susceptibility target has implications in genome editing for novel plant resistance against devast

124 ween RNA interference (RNAi) and CRISPR-Cas9 genome editing for perturbation of gene expression using

125 n of most aquaculture species can facilitate genome editing for research and application at a scale t

126 sed genotyping platforms, coupled with rapid genome editing for variant validation.

127 The ODInCas9 mouse allows robust and tunable genome editing granting flexibility, speed and uniformit

128 Recently, CRISPR/Cas9 genome editing has been shown capable of generating inve

129 of possible molecular events resulting from genome editing has been underestimated and the technolog

130 CRISPR-based genome editing has enabled large-scale functional geneti

131 Genome editing has powerful applications in research, he

132 CRISPR/Cas9 genome editing has revolutionized functional genomics in

133 Genome editing has the potential to treat an extensive r

134 CRISPR-Cas9 genome editing has transformed biotechnology and therape

135 nce "Recent innovations in CRISPR-Cas9-based genome editing have facilitated such genetic interaction

136 Although recent improvements in genome editing have made it possible to directly modify

137 Genome editing holds great potential for correcting path

138 nificant implications for the application of genome editing in both basic research and clinical pract

139 predicted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a multiplexed

140 ide a safer or more effective alternative to genome editing in certain clinical scenarios.

141 increase the efficiency of CRISPR-Cas9-based genome editing in clinically relevant primary cell types

142 Here, we use standard and multiplex genome editing in conjunction with functional and behavi

143 hort palindromic repeats (CRISPR) system for genome editing in eukaryotes has revolutionized basic bi

144 ction and can also block CRISPR-Cas-mediated genome editing in eukaryotic cells.

145 Mutant BhCas12b facilitates robust genome editing in human cell lines and ex vivo in primar

146 (CRISPR)/CRISPR-Associated-9 (Cas9)-mediated genome editing in human pluripotent stem (PS) cells main

147 osed for efficient chromosomal targeting and genome editing in Lactobacillus crispatus, an important

148 CRISPR/Cas systems have been engineered for genome editing in many organisms, including plants.

149 nation of human association analysis, CRISPR genome editing in mice, animal behavioural analysis and

150 s as well as extremely efficient and precise genome editing in P patens Additionally, careful phyloge

151 eover, these strategies have been applied to genome editing in preclinical research and clinical tria

152 Here we establish CRISPR/Cas9-mediated genome editing in S. rosetta by engineering a selectable

153 er research using hPSC-derived organoids and genome editing in the future.

154 as9 expression and achieved highly efficient genome editing in the presence of guide RNA.

155 /Cas9 nucleases have been widely applied for genome editing in various organisms.

156 An effective and very fast CRISPR/Cas9 genome editing in vitro and in vivo enabled by bioreduci

157 RNP-loaded SMOF NPs induced efficient genome editing in vivo in murine retinal pigment epithel

158 ates to perform homology directed repair and genome editing in vivo.

159 CRISPR/Cas9 has become a powerful tool for genome editing in zebrafish that permits the rapid gener

160 Genome-editing in human embryonic stem cell-derived card

161 ls of off-target effects can be tolerated in genome editing, in the context of various types of appli

162 In vitro genome editing indicated that the SRF enhancer CArG box

163 CRISPR-Cas genome editing induces targeted DNA damage but can also

164 Genome editing is a method for making targeted sequence

165 nt standard for algal research, and although genome editing is still far from efficient and routine,

166 One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) bindi

167 A key challenge in precise genome editing is the low efficiency of homology-directe

168 Genome editing is-or will soon be-in the clinic for seve

169 de), nucleic acids (e.g., DNA and mRNA), and genome-editing machineries (e.g., Cas9-sgRNA ribonucleop

170 targeted and cell-specific delivery of this genome editing machinery.

171 An enhancer trap line built by genome editing marked Tc-foxQ2 positive neurons, which p

172 its postcatalytic conformation may influence genome editing mechanisms.

173 developmentally programmed phenomena such as genome editing mediated epigenetically by RNA, as well d

174 cribe prime editing, a versatile and precise genome editing method that directly writes new genetic i

175 Development of genome editing methodologies began ~20 years ago, first

176 Due to breakthroughs in RNAi and genome editing methods in the past decade, it is now eas

177 Using genome editing methods, we disrupt uridine monophosphate

178 bining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF

179 ted RAS pathways, offering a new therapeutic genome-editing modality for the colorectal cancer treatm

180 , including screens that harness CRISPR/Cas9 genome editing, natural genetic variation, proteomics, a

181 ction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its

182 ses and may be particularly advantageous for genome editing of C. cellulolyticum H10, a bacterium con

183 peutic strategies for HIV infection based on genome editing of CCR5.

184 study lays the foundation for nontransgenic genome editing of citrus.

185 R guide RNA libraries, which can be used for genome editing of coding and non-coding genomic regions

186 Genome editing of human pluripotent stem cells (hPSCs) p

187 Genome editing of the mutated gene may be envisaged as a

188 hts aspects of host response and alternative genome editing outcomes that require further study.

189 Even with high efficiency levels of genome editing, phenotypes may be obscured by proportion

190 f a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses un

191 ther study to advance retron-based precision genome editing platforms.

192 9), an alternative to the most commonly used genome editing protein Streptococcus pyogenes Cas9 (SpyC

193 be considered when selecting the method for genome-editing reagent delivery in plants, and emphasize

194 An alternative is to introduce genome editing reagents and a homologous recombination (

195 hat using computational algorithms to design genome editing reagents can mitigate off-target edits in

196 Here, we introduced genome editing reagents into single-cell bovine embryos

197 at transient inhibition of TP53 may increase genome editing recovery in primary and TP53+/+ cell line

198 ncer cell lines through CRISPR/Cas9-mediated genome editing reduces HDL uptake into the prostate canc

199 ing, and the safety and efficacy concerns of genome editing remain.

200 ucible transgenesis, or CRISPR/Cas9-mediated genome editing, reproduced DM1-related cardiac conductio

201 However, traditional CRISPR/Cas9-mediated genome editing requires plant tissue culture that is bot

202 n of our strategy using one-step CRISPR-Cas9 genome editing restructured vine-like tomato plants into

203 hairpin RNA (shRNA) depletion or CRISPR/Cas9 genome editing resulted in impaired mitochondrial functi

204 Indeed, CRISPR-Cas9 genome editing revealed that the single hodor orthologue

205 em after CRISPR-Cas treatment, and on-target genome editing side effect in undesired tissues.

206 y, disruption of GAL5.1 in mice using CRISPR genome editing significantly reduced GAL expression in t

207 Early genome editing strategies involved many naturally occurr

208 y accelerated the development of therapeutic genome editing strategies that are based on either the k

209 ical information to facilitate the design of genome-editing strategies, showcase the past and future

210 obtained by direct chromosomal tagging using genome-editing strategies.

211 Here we show that a CRISPR/Cas9-based genome editing strategy allows the precise correction of

212 ic acid delivery fields have been crucial to genome editing success to date, including adeno-associat

213 spin-knockout cells generated by CRISPR/Cas9 genome editing, suggesting "off-target" effects.

214 RISPR/Cas, are the components of a bacterial genome editing system that can be used to perturb genes

215 The recent advent of genome editing technologies (especially CRISPR, Clustere

216 With the emergence of genome editing technologies and synthetic biology, it is

217 es for S. viridis research, highly efficient genome editing technologies are needed to create genetic

218 The current generation of genome editing technologies is already proving useful to

219 nical demonstrations, and recent advances in genome editing technologies may enable the use of report

220 ral processes, including DNA repair, leading genome editing technologies rely on nuclease activity, i

221 t critical step in the future translation of genome editing technologies.

222 With the rapid development of new genome-editing technologies and the availability of incr

223 Recent advances in genomics and genome-editing technologies are elevating the study of e

224 entic human beta-cell lines, and advances in genome-editing technologies coupled with improved protoc

225 interspaced short palindromic repeats)-based genome editing technology is an emerging RNA-guided nucl

226 The advent of genome editing technology provides new opportunities to

227 Using biochemical methods and genome editing technology, here we show that WOX9 is an

228 e natural toolbox for regulating CRISPR-Cas9 genome editing temporally, spatially, and conditionally.

229 We present ampliCan, an analysis tool for genome editing that unites highly precise quantification

230 Using CRISPR/Cas9 genome editing, the enhancer cluster or parts thereof, N

231 With recent advances in genome editing, this type of genetic mutation can be pre

232 Genome editing to correct a defective beta-globin gene o

233 We used CRISPR/Cas9-genome editing to create isogenic cellular models.

234 Using genome editing to delete candidate REs, we showed that a

235 We then use genome editing to disrupt the coding sequence of a S. ro

236 Here we use genome editing to engineer a general platform to improve

237 We used CRISPR/Cas9-mediated genome editing to functionally define a bipartite bounda

238 We used genome editing to generate a Gdf15 (nuGFP-CE) mouse line

239 ircle amplification and CRISPR/Cas9-mediated genome editing to generate Dmpk CTG expansion (CTG(exp))

240 To circumvent that, we used genome editing to generate isogenic human embryonic stem

241 orporating advances in imaging, genomics and genome editing to identify key cell types and molecules

242 The potential of genome editing to improve the agronomic performance of c

243 The use of CRISPR-Cas9 genome editing to introduce endogenously expressed tags

244 We used genome editing to model NCRNA in mice.

245 Genome editing to site-specifically disrupt the Hoxa9-ES

246 Here we employed multiplex genome editing to specifically ablate the expression of

247 ment of cobalamin metabolic enzymes, we used genome editing to study the loss of mmachc function and

248 ort palindromic repeats-associated protein 9 genome editing to study the role of microtubules and mic

249 When genome editing to substitute mitosis for meiosis (MiMe)(

250 The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles di

251 Using the CRISPR-Cas9 genome editing tool combined with the Nanopore Sequencin

252 The CRISPR/Cas9 genome editing tool has the potential to improve the liv

253 re, we describe an optimized Cas9-AAV6-based genome editing tool platform for site-specific mutagenes

254 guided CRISPR/Cas9 technology is a versatile genome editing tool that has revolutionized targeted mut

255 CRISPR/Cas9 is a programmable genome editing tool widely used for biological applicati

256 onuclease, which has become the most popular genome editing tool.

257 in 9 (Cas9) system has emerged as a powerful genome-editing tool to correct genetic disorders.

258 ) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes.

259 CRISPR-Cas9 is a widely employed genome-editing tool with functionality reliant on the ab

260 CRISPR/Cas9 is a powerful genome-editing tool, but spurious off-target edits prese

261 ntages for cellular delivery that expand the genome editing toolbox.

262 Here, we have developed multiplex genome editing toolkits for citrus including PEG-mediate

263 We have developed multiplex genome editing toolkits for citrus that significantly im

264 detect potential off-target variants of any genome editing tools by the combination of experimental

265 The development of new CRISPR-Cas genome editing tools continues to drive major advances i

266 ide to evaluate the side effects of emerging genome editing tools on cells and organisms.

267 Further, iPSC-based systems and genome editing tools will be critical in understanding t

268 iral defence systems have been repurposed as genome editing tools.

269 cal for accurate evaluation of the safety of genome editing tools.

270 s potential for application as modulators of genome editing tools.

271 nd social challenges faced by breeders using genome-editing tools for crop improvement.

272 Recent advances in CRISPR present attractive genome-editing toolsets for therapeutic strategies at th

273 in natural populations and in the context of genome editing-toward improving plant growth under Pi-de

274 Genome editing typically involves recombination between

275 Precise genome editing using CRISPR-Cas9 is a promising therapeu

276 Genome editing using CRISPR/Cas9 was used to introduce p

277 Genome editing using programmable nucleases is revolutio

278 , we demonstrate its application for precise genome editing using single strand DNA oligonucleotides

279 tivated elsewhere, gene delivery and in vivo genome editing via MNP-BVs are tissue specific.

280 The field of genome editing was founded on the establishment of metho

281 Finally, genome editing was used to explore the functional conseq

282 dysfunction in these syndromes, CRISPR/Cas9 genome editing was used to generate human pluripotent st

283 Using CRISPR/Cas9-mediated genome editing, we demonstrate that CSK is essential for

284 ng stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct NANOG tar

285 By CRISPR/Cas9 genome editing, we knocked out AH1 and saw that only nec

286 By combining quantitative genetics with genome editing, we show how multiple SVs that changed ge

287 -immunoprecipitation assays, and CRISPR/Cas9 genome editing, we show that Drp1 phosphorylated at Ser-

288 Using genome editing, we tagged 29 BM matrix components and re

289 With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLes1) a

290 ssociated virus (AAV) approach, coupled with genome editing, we validated that GCK-IV kinase knockout

291 By utilizing genome editing, we verify that identified functional var

292 elative levels of successful heritable plant genome editing were addressed using simple case studies

293 Mice in which Nppb was deleted by genome editing were subjected to myocardial infarction.

294 the emergence of high-throughput assays and genome editing, which are switching the paradigm from bo

295 Genome editing, which involves the precise manipulation

296 Genome editing will likely undergo improved editing spec

297 factors to double-strand breaks (DSBs) after genome editing with CRISPR nucleases.

298 vity of Cas12i could potentially be used for genome editing with high specificity.

299 inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable le

300 ection of PfEBA165 inactivating mutations by genome editing yields viable parasites, but is associate