ライフサイエンスコーパス: geranylgeranylation

1 GTPase (membrane anchored via its C-terminal geranylgeranylation).

2 can be modified in vitro by farnesylation or geranylgeranylation.

3 lysine region are important determinants for geranylgeranylation.

4 ed, all other gamma subunits are modified by geranylgeranylation.

5 not due to failure of the mutant to undergo geranylgeranylation.

6 ce on one mevalonate pathway output, protein geranylgeranylation.

7 signals via mevalonate metabolism to protein geranylgeranylation.

8 ), which is post-translationally modified by geranylgeranylation.

9 y provide valuable reagents to study protein geranylgeranylation.

10 ve DAF expression is negatively regulated by geranylgeranylation.

11 t affected by substituting farnesylation for geranylgeranylation.

12 vents seem to be downstream of inhibition of geranylgeranylation.

13 protein prenylation as shown by protein RhoA geranylgeranylation.

14 escribed Rho family proteins are modified by geranylgeranylation, a lipid attachment required for pro

15 Rab activity is dependent upon geranylgeranylation, a post-translational modification t

16 However, how RhoGTPases are regulated by geranylgeranylation, a post-translational modification,

17 lonate pathway, is the substrate for protein geranylgeranylation, a protein post-translational modifi

18 gh binding of Cdc42 to PLD1 does not require geranylgeranylation, activation of PLD1 is dependent on

19 Inhibition of its geranylgeranylation affords a therapeutic strategy for t

20 errogating biologies associated with protein geranylgeranylation and define a novel structure for thi

21 n of the TRQQKRP motif results in diminished geranylgeranylation and delocalization of intracellular

22 Lovastatin acted by inhibiting both geranylgeranylation and farnesylation, and not by alteri

23 esol pyrophosphate and thereby inhibits both geranylgeranylation and farnesylation.

24 talyzed farnesylation is 37-fold slower than geranylgeranylation and is limited by the farnesyl trans

25 Geranylgeranylation and membrane association of Rab27, a

26 ered with angiogenesis via inhibition of the geranylgeranylation and membrane localization of RhoA.

27 ough lovastatin, which inhibits both protein geranylgeranylation and protein farnesylation, blocked P

28 Our data indicate that inhibition of protein geranylgeranylation and RhoA pathways induce apoptosis i

29 The role of protein geranylgeranylation and the RhoA pathway in the regulati

30 ndent post-translational lipid modification (geranylgeranylation) and subsequent membrane association

31 ins simultaneously inhibit farnesylation and geranylgeranylation, and in consequence do not inhibit M

32 hich inhibits both protein farnesylation and geranylgeranylation, arrested cells in G0/G1 whereas cel

33 by geranylgeranyl pyrophosphate, implicating geranylgeranylation as a critical determinant of the sta

34 corresponding isoprenols, abolishes protein geranylgeranylation as well as GGOH-induced ERAD of redu

35 Post-translational farnesylation or geranylgeranylation at a C-terminal cysteine residue reg

36 on tendon cell expansion is specific to the geranylgeranylation branch of the mevalonate pathway and

37 or tyrosine phosphorylation requires protein geranylgeranylation but not protein farnesylation and th

38 These results demonstrate that protein geranylgeranylation, but not farnesylation, is required

39 atin inhibits both protein farnesylation and geranylgeranylation by decreasing cellular pools of farn

40 ulation of iNOS by statins via inhibition of geranylgeranylation by GGTI-298, but not via inhibition

41 d with the C-terminal peptide of lamin B for geranylgeranylation by PGGT-I and for farnesylation by P

42 lation of leucine-ending CAAX substrates nor geranylgeranylation by the FTase is necessary for these

43 The selective inhibition of protein geranylgeranylation by the specific protein geranylgeran

44 ed by FTase to a level comparable to that of geranylgeranylation catalyzed by GGTase I.

45 In the absence of protein geranylgeranylation, compromised PI(3)K activity allows

46 phosphate, we demonstrate that the impact of geranylgeranylation depends on the fatty acid content of

47 In contrast, geranylgeranylation down-regulates MMP-1 expression.

48 Thus, inhibition of geranylgeranylation either directly through geranylgeran

49 Consistent with a role in geranylgeranylation, embryos deficient in geranylgeranyl

50 We found that protein geranylgeranylation enabled Toll-like receptor (TLR)-ind

51 The authors found that loss of the geranylgeranylation enzyme geranylgeranyl transferase ty

52 Swapping geranylgeranylation for farnesylation on Ras proteins or

53 1 require post-translational modification by geranylgeranylation for full function.

54 rdial cell-autonomous requirement of Ggamma1 geranylgeranylation for heart formation and suggest the

55 Protein geranylgeranylation (GGylation) is an important biochemi

56 ontaining a C-terminal CAAX motif to promote geranylgeranylation (GRK6-GG).

57 Although geranylgeranylation has been shown to regulate activitie

58 ause we have shown that targeting Rab GTPase geranylgeranylation impairs monoclonal protein trafficki

59 hile 3-vinylgeranylgeraniol restores protein geranylgeranylation in cells.

60 sult calls into question the role of protein geranylgeranylation in inflammatory cell signaling.

61 been attributed to inhibition of RHO protein geranylgeranylation in inflammatory cells.

62 n, O-cholesterylation, S-farnesylation and S-geranylgeranylation in multiple cell lines to illustrate

63 tional lipid modifications farnesylation and geranylgeranylation in protein localization and function

64 g perturbations in farnesylation rather than geranylgeranylation in synergistic interactions.

65 These findings highlight the importance of geranylgeranylation in the dynamic management of RhoGTPa

66 Rap 1A; the lack of Rap 1A processing beyond geranylgeranylation in the variant cells was not seconda

67 sults highlight a conserved role for protein geranylgeranylation in this context.

68 s of protein prenylation and unique roles of geranylgeranylation in thymic egress and highlight that

69 y we demonstrated that inhibition of protein geranylgeranylation inhibited phorbol ester-stimulated a

70 further mutated to undergo posttranslational geranylgeranylation is also more active, recovering most

71 Protein geranylgeranylation is critical for the function of a nu

72 Geranylgeranylation is critical to the function of sever

73 In this study, I show that the kinetics of geranylgeranylation is influenced by the nucleotide stat

74 Geranylgeranylation is initiated by formation of a stabl

75 These findings indicate that geranylgeranylation is of crucial importance for the mai

76 This geranylgeranylation is required for their proper subcell

77 lational modification of the Rho proteins by geranylgeranylation is required for their subsequent act

78 Prenylation of protein (farnesylation and geranylgeranylation) is involved in several human cancer

79 n addition to bypassing FTI blockade through geranylgeranylation, K-Ras4B resistance to FTIs may also

80 H 3T3 and Rat-1 cells) inhibition of protein geranylgeranylation leads to a G0/G1 arrest, whereas inh

81 anylgeranylated and that inhibition of their geranylgeranylation mediates, at least in part, the effe

82 able which can distinguish farnesylation and geranylgeranylation modification in a single experimenta

83 ne) while showing little preference for CAAL geranylgeranylation motif substrates (where L represents

84 pene blocks an MEP pathway-dependent protein geranylgeranylation necessary for the signaling cascade.

85 nesol, further confirming that inhibition of geranylgeranylation, not farnesylation, is responsible f

86 In vitro geranylgeranylation of an Arabidopsis Rab1 protein conta

87 Geranylgeranylation of C17orf37 at the CAAX motif facili

88 he fluorescence enhancement that accompanies geranylgeranylation of dansyl-GCIIL.

89 FTase was also able to catalyze geranylgeranylation of GST-lHDAg at a very low rate, sug

90 y low rate, suggesting that the low level of geranylgeranylation of GST-lHDAg observed in cytosolic p

91 Geranylgeranylation of lHDAg expressed in COS cells was

92 bly of the viral replication complex require geranylgeranylation of one or more host proteins.

93 Geranylgeranylation of Rab GTPases is an essential post-

94 These findings indicate that geranylgeranylation of Rab GTPases is critical for hemos

95 an enzyme responsible for post-translational geranylgeranylation of Rab GTPases represents one way to

96 l blocked the ability of insulin to increase geranylgeranylation of Rab-4, whereas GGTI-298 and alpha

97 n cognate to Ras Gln-61.2) Posttranslational geranylgeranylation of Rab24, determined by metabolic la

98 Geranylgeranylation of Rabs is a complex reaction that r

99 where the inhibitors were shown to block the geranylgeranylation of Rap-1A without affecting the farn

100 geranyl pyrophosphate, the substrate for the geranylgeranylation of Rho, reversed the effect of simva

101 hibited by GGTI, a specific inhibitor of the geranylgeranylation of Rho; by C3 exotoxin, which inacti

102 Geranylgeranylation of RhoA small G-protein is essential

103 This leads to reduced geranylgeranylation of small GTPases such as Rho and Rac

104 e (100 micromol/L), suggesting inhibition of geranylgeranylation of target protein(s) as the predomin

105 -lowering drug that blocks farnesylation and geranylgeranylation of target proteins, increases LPS-in

106 n the sense that it is strongly inhibited by geranylgeranylation of the adjacent cysteine.

107 Geranylgeranylation of the fusion protein was also obser

108 senescence in NHOK is mediated, in part, via geranylgeranylation of the mevalonate pathway.

109 mma1 CaaX sequence (gamma1-S74L) resulted in geranylgeranylation of the resulting gamma1 subunit, and

110 An escort protein, Msi4p, was necessary for geranylgeranylation of Ypt1p by yeast PGGTase-II.

111 n requires a posttranslational modification, geranylgeranylation, of the C-terminal cysteine residue.

112 ies addressing which isoprenylation pathway--geranylgeranylation or farnesylation--is inhibited by si

113 pyrophosphate and mimicked by inhibiting Rho geranylgeranylation or Rho-kinase (ROCK).

114 her peptides are specific for farnesylation, geranylgeranylation, or dual prenylation.

115 e pharmacological shunting of K-Ras into the geranylgeranylation pathway promotes K-Ras association w

116 ut upstream of cholesterol, specifically the geranylgeranylation pathway.

117 prenylation by impeding their entry into the geranylgeranylation pathway.

118 ct on TGFbeta signaling by inhibition of the geranylgeranylation pathway.

119 hese results document that farnesylation and geranylgeranylation play differential roles in AD pathog

120 presence of lovastatin, to stimulate protein geranylgeranylation, prevented lovastatin's effects.

121 e found that by supplying more substrate for geranylgeranylation, Rac1 activation was substantially i

122 Furthermore, the geranylgeranylation rate constant is enhanced by the add

123 om dissociating from REP prior to the second geranylgeranylation reaction, ensuring efficient digeran

124 Third, we show that free REP inhibits the geranylgeranylation reaction, suggesting that the comple

125 Here, we studied the role of REP in the geranylgeranylation reaction.

126 However, the catalytic efficiencies of these geranylgeranylation reactions are between 15- and 140-fo

127 The isoprenoid donor for protein geranylgeranylation reactions, geranylgeranyl diphosphat

128 Statin treatment inhibiting Rac1 geranylgeranylation reduced 11beta-HSD2 up-regulation.

129 ermine whether protein prenylation (farnesyl/geranylgeranylation) regulates matrix metalloproteinase

130 g the therapeutic consequences of inhibiting geranylgeranylation relative to farnesylation.

131 Geranylgeranylation represents a novel mechanism by whic

132 addition of geranylgeraniol, which restores geranylgeranylation, rescued HUVEC from apoptosis.

133 selectively block protein farnesylation and geranylgeranylation, respectively.

134 In cultured podocytes, the absence of geranylgeranylation resulted in altered activity of its

135 Geranylgeranylation's effects on RhoGTPases and Rap1 fun

136 To define geranylgeranylation's role in podocytes, we generated po

137 Posttranslational geranylgeranylation selectively alters the lifecycle of

138 We propose that post-translational geranylgeranylation serves as a regulator of both RhoA a

139 1 or Sso2 proteins that have a COOH-terminal geranylgeranylation signal instead of a transmembrane do

140 fect resulted from the inhibition of protein geranylgeranylation subsequent to the depletion of meval

141 gin have higher levels of proteins requiring geranylgeranylation than arterial endothelial cells and

142 However, elevated geranylgeranylation was not Rab5a-specific since all the

143 gh the classical Rho GTPases are modified by geranylgeranylation, we found that a majority of the oth

144 potentiate the induced-inhibition of protein geranylgeranylation when used in a 3:1 HG:HN combination

145 synthesis, which blocks cytokinesis, and in geranylgeranylation, which interferes with progression t

146 up-regulate eNOS expression by blocking Rho geranylgeranylation, which is necessary for its membrane

147 is caused by lipid depletion limits RAB-11.1 geranylgeranylation, which promotes nuclear translocatio

148 ts as a highly specific inhibitor of protein geranylgeranylation, while 3-vinylgeranylgeraniol restor

149 Furthermore, inhibition of protein geranylgeranylation with GGTI-298 significantly induced