ライフサイエンスコーパス: germ

1 tiles can be created from asymmetrical tooth germs.

2 eatments due to the faster growth of control germs.

3 Further, abnormal phalloidin and wheat germ agglutinin (WGA) staining of Tdrd7-/- fiber cells,

4 ed to an approximately 50% decrease in wheat germ agglutinin (WGA)-labeled components of the GCX unde

5 es, the complete dynamic genetic programs of germ and granulosa cells from E16.5 to postnatal day (PD

6 identify transcriptional signatures of major germ and somatic cell types of the testes in human, maca

7 extracts prepared from budding yeast, wheat germ, and rabbit reticulocyte lysates.

8 Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilate

9 tion and direct responsiveness to primordial germ cell (PGC) specification, a unique functional featu

10 onial specification whereby human primordial germ cell (PGC)-like cells differentiated from human ind

11 ated multi-organ in vitro systems to support germ cell and embryo function, and to display characteri

12 perates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT s

13 as carriers of epigenetic information during germ cell and pre-implantation development by ensuring t

14 llected during multiple stages of primordial germ cell and pre-implantation development, we find that

15 Drosophila ovary is a widely used model for germ cell and somatic tissue biology.

16 In mouse fetal testes, the majority of germ cell apoptosis coincides with the onset of male dif

17 628 in the mouse and uncovered a postmeiotic germ cell arrest at the round spermatid stage in the sem

18 re, we use our current understanding of male germ cell biology and TLR function as a starting point t

19 Finally, distinct differentiating germ cell cyst samples do not exhibit obvious dosage com

20 to other sites of open chromatin, leading to germ cell death and sterility.

21 nt in parental chromosomes during primordial germ cell development and after fertilization.

22 ture targeted mechanistic studies of primate germ cell development and in vitro gametogenesis.

23 as a multifunctional hub for haematopoiesis, germ cell development and nutritional supply.

24 ight the importance to understand primordial germ cell development and the timing of gametogenesis wi

25 s to understand the mechanisms that regulate germ cell development opens promising new avenues to dev

26 cytokines, and other biomolecules to support germ cell development.

27 DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from har

28 1 and ADAD2 are essential regulators of male germ cell differentiation with molecular functions unrel

29 D1 and ADAD2, on testis RNA editing and male germ cell differentiation.

30 A disproportionate number of germ cell divisions were observed at the DTC-Sh1 interfa

31 , suggesting that epigenetic modification of germ cell DNA may mediate transgenerational transmission

32 uman cohorts to identify changes in paternal germ cell epigenetics in association with offspring dise

33 script profile of two cell states expressing germ cell factors, one we conclude represents the primor

34 t adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbi

35 with P granule exit for two mRNAs coding for germ cell fate regulators.

36 d that PRDM14 might be dispensable for human germ cell fate.

37 s evolved to fulfil a crucial role in insect germ cell formation.

38 ell resulting from a reprogrammed primordial germ cell from the thymus.

39 ation, chromosome segregation, and repair of germ cell genomes remain incompletely understood.

40 type cells rescued gonad development but not germ cell induction in Tfap2 mutants.

41 ormative stem (FS) cells respond directly to germ cell induction.

42 However, no germ cell intrinsic mechanism that protects from heat ha

43 eloped a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis us

44 ructure, blocks differentiation and promotes germ cell loss, phenotypes that are partially rescued by

45 tion activates the NL checkpoint that causes germ cell loss.

46 lure of the maintenance and/or activation of germ cell markers and pluripotency genes.

47 als involved in cellular function, including germ cell maturation.

48 Here we demonstrate, using primordial germ cell migration in mouse as a developmental model, t

49 ratory processes, including inflammation and germ cell migration, means that self-generated gradients

50 We identified Germ Cell Nuclear Acidic Peptidase (GCNA) as a conserved

51 iosis results in heterogeneity in the female germ cell populations, which limits the studies of meios

52 for germline development to pole cells, the germ cell progenitors.

53 erse environmental factors able to influence germ cell programming and potentially impact offspring d

54 'intersex' or male phenotype may compromise germ cell progression into meiosis, causing cortical ger

55 2/3 perturbed the DTC-Sh1 interface, reduced germ cell proliferation, and shifted a differentiation m

56 o assess the impact of Adad mutation on male germ cell RNA editing, CRISPR-induced alleles of each we

57 a role for both in meiotic and post-meiotic germ cell RNA editing.

58 Maintenance of germ cell sexual identity is essential for reproduction.

59 Here, we identify the germ cell specific Golgi glycoprotein MGAT4D as a protec

60 rence of metachronous contralateral (second) germ cell testicular cancer (TC).

61 atoma in patients with metastatic testicular germ cell tumor (GCT) is of unknown prognostic significa

62 90 to 0.96), was lower after nonseminomatous germ cell tumor (HR, 0.58; 95% CI, 0.35 to 0.96) and dec

63 Patients with testicular germ cell tumor (TGCT) are at increased risk of developi

64 a +/- necrosis (20%), viable nonteratomatous germ cell tumor +/- teratoma (41%), and secondary somati

65 oogenesis, leading to either an agametic or germ cell tumor phenotype.

66 Germ cell tumors (GCTs) are the most common cancer in me

67 Some germ cell tumors (GCTs) in men develop into hematologic

68 e management of central nervous system (CNS) germ cell tumors (GCTs).

69 iomarkers to differentiate non-germinomatous germ cell tumors (NGGCTs) from germinomas are critical,

70 th pathologic stage (PS) IIA nonseminomatous germ cell tumors (NSGCTs) is 10%-20% but increases to >=

71 ry networks in two major types of testicular germ cell tumors (TGCT): seminoma (SE) and non-seminoma

72 y associated with lymphomas and some ovarian germ cell tumors, we present a case of calcitriol overpr

73 bility in flies, worms, zebrafish, and human germ cell tumors.

74 While most testicular germ cell tumours (TGCTs) exhibit exquisite sensitivity

75 ovarian cancers, including malignant ovarian germ cell tumours, sex cord-stromal tumours, and small c

76 d capable of differentiation into primordial germ cell-like cells.

77 We show that germ cell-specific BAF knockdown causes phenotypes that

78 Mice with germ cell-specific Klotho (gcKL) deficiency neither had

79 t al., 2020) reveal an essential function of germ cell-specific protein GCNA in the genome maintenanc

80 stulated as likely candidates for sustaining germ cell-specific transcription programs throughout the

81 pports our hypothesis that earlier errors in germ-cell epigenetic reprogramming derail differentiatio

82 Our results indicate that the fetal germ-cell fate is based on discrete cell-heritable ident

83 We studied fetal germ-cell fates and discovered that both apoptosis and d

84 cytological and scRNAseq analyses identified germ-cell intrinsic and extrinsic genes responsive to Se

85 The human germ-cell lineage originates as human primordial germ ce

86 Germ-cell transcription factors control gene networks th

87 from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo

88 that genetic rescue of DNA repair-deficient germ cells (Fancm(-/-) ) leads to increased mutation inc

89 -cell lineage originates as human primordial germ cells (hPGCs).

90 4 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripo

91 a lesser extent in Mgat4d[-/-] heat-stressed germ cells (NFkappaB response, TNF and TGFbeta signaling

92 we investigated niche wrapping of primordial germ cells (PGCs) in the C. elegans embryonic gonad prim

93 These primordial germ cells (PGCs) undergo rapid proliferation, yet the g

94 the functional role of piwil2 in primordial germ cells (PGCs) was investigated in Nile tilapia using

95 l migration program of Drosophila primordial germ cells (PGCs), we show that cluster dispersal is acc

96 ection of plasmids in circulating Primordial Germ Cells (PGCs).

97 l CpG methylation drops to 10% in primordial germ cells and 20% in the inner cell mass of the blastoc

98 ied 5 distinct cell clusters associated with germ cells and 6 with granulosa cells.

99 play a significant and multifaceted role in germ cells and development.

100 functions of phase-separated compartments in germ cells and examine the various ways in which phase s

101 TEX15 is expressed in embryonic germ cells and functions during genome-wide epigenetic r

102 bryonic sac, and specification of primordial germ cells and gastrulating cells (or mesendoderm cells)

103 tinia symbiolongicarpus Tfap2 mutants lacked germ cells and gonads.

104 each specific stage, and the interactions of germ cells and granulosa cells basing on known and novel

105 f the biological process most represented in germ cells and granulosa cells or common to both cell ty

106 e previously reported expression of genes by germ cells and granulosa cells, our analyses identified

107 lausible because KLOTHO is expressed in both germ cells and spermatozoa and forms with FGFR1 a specif

108 LOTHO and FGFR1 were also expressed in human germ cells and spermatozoa, and FGF23 treatment augmente

109 s, one we conclude represents the primordial germ cells and the other state is transiently present du

110 diated deletion of W38 in chicken primordial germ cells and the successful production of the gene-edi

111 onverts to the germ line when the primordial germ cells are deleted.

112 Many germ cells are eliminated during development, long befor

113 jority of genes under PHF7 control in female germ cells are not under PHF7 control in male germ cells

114 Male germ cells are sensitive to heat stress and testes must

115 Germ cells are vulnerable to stress.

116 assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein

117 demonstrate how niche cell wrapping protects germ cells by manipulating their signaling environment a

118 to alter chromatin in soon-to-be fertilized germ cells by recruiting the histone chaperone FACT, dis

119 r the specialized maturation of haploid male germ cells called spermiogenesis.

120 Germ cells clustered into six meiotic substages, as well

121 he transcriptional profiles of 19 363 single germ cells collected from E12.5, E14.5, and E16.5 mouse

122 Lineage tracing confirmed that germ cells die as clones independent of intercellular br

123 AT4D is a novel, intrinsic protector of male germ cells from heat stress.

124 i glycoprotein MGAT4D as a protector of male germ cells from heat stress.

125 their signaling environment and by shielding germ cells from unwanted cellular interactions that can

126 This work shows that GCNA protects germ cells from various sources of damage, providing ins

127 ogenesis is the process by which uncommitted germ cells give rise to haploid sperm.

128 amined whether XCD extends to human prenatal germ cells given their similarities to naive pluripotent

129 ignaling pathways acting between somatic and germ cells in a stage-specific manner during the perinat

130 Developing germ cells in Drosophila have an additional specialized

131 otransposons must be activated in developing germ cells in order to survive and propagate, how they a

132 HLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation drama

133 ts that reduce coupling between the soma and germ cells in the Caenorhabditis elegans gonad.

134 anes, P granules (associated with progenitor germ cells in the P lineage) and P-bodies (associated wi

135 2 or 12-Lox activity also inhibits homing of germ cells in vivo Using a live-imaging chemotaxis assay

136 Here, we show that PHF7-expressing ovarian germ cells inappropriately express hundreds of genes, ma

137 ntricate crosstalk between Sertoli cells and germ cells including spermatogonia, spermatocytes, haplo

138 Repression of cellular reprogramming in germ cells is critical to maintaining cell fate and fert

139 Germ cells lacking Mgat4d generally mounted a similar he

140 is study reveals that ectopic PHF7 in female germ cells leads to a loss of sexual identity and the pr

141 cient CRISPR/Cas9 gene editing in primordial germ cells represents a substantial addition to genotech

142 l and electron microscopy of HIPK4-null male germ cells reveals defects in the filamentous actin (F-a

143 Germ cells specified during fetal development form the f

144 e incidence may be much higher in developing germ cells than in zygotes.

145 ed transcriptional heterogeneity among fetal germ cells that included an apoptosis-susceptible popula

146 s coupled to transcription response times of germ cells to protect future offspring.

147 agation of species depends on the ability of germ cells to protect their genome from numerous exogeno

148 l progression into meiosis, causing cortical germ cells to remain in an immature state in the embryo.

149 of cellular differentiation from primordial germ cells toward meiocytes.

150 laying severe teratospermia and Adad2 mutant germ cells unable to progress beyond round spermatid.

151 nd 1 branch point of fate transition for the germ cells were revealed, as well as for the granulosa c

152 sed in tissues, others are expressed in only germ cells with aberrant reactivation in multiple cancer

153 ess of the testis (43 degrees C for 25 min), germ cells with inactivated Mgat4d were markedly more se

154 s whose expression is normally restricted to germ cells yet aberrantly activated in tumors, where the

155 e novo mutations examined pedigrees (and not germ cells) and thus were likely affected by selection.

156 -protein diet elevates ROS in the testicular germ cells, altering ATF7 activity and H3K9me2 abundance

157 rophase state (leptotene/zygotene) in mutant germ cells, and identified several misregulated genes in

158 ted a similar heat shock response to control germ cells, but could not maintain that response.

159 e formed without specification of primordial germ cells, epigenetic reprogramming or meiosis, and dem

160 For example, in Drosophila female germ cells, forced expression of the testis-specific PHD

161 Integration also occurs in germ cells, meaning that the virus can be inherited and

162 f alms1a in GSCs, but not in differentiating germ cells, results in rapid loss of centrosomes due to

163 erm cells are not under PHF7 control in male germ cells, suggesting that PHF7 is acting in a tissue-s

164 al neurons, FACT is not recruited by HSF1 in germ cells, transcription occurs but is delayed, and pro

165 st, ectoderm, mesoderm, endoderm, primordial germ cells, trophectoderm, and amnion.

166 ng transcription factors expressed in female germ cells, we analyzed global gene expression profiles

167 ranscriptome features of peri-meiotic female germ cells, which offers new information not only on mei

168 pacity to undergo meiosis defines vertebrate germ cells, yet mechanisms driving initiation of this sp

169 ic protein GCNA in the genome maintenance of germ cells.

170 xpressed on GSCs and differentiating vasa(+) germ cells.

171 eding epigenetic reprogramming in primordial germ cells.

172 ns predominantly as a regulator of Dnmt3a in germ cells.

173 gnals in the periphery that communicate with germ cells.

174 y altered chromatin structure in postmeiotic germ cells.

175 ession of Tfap2 in i-cells converted them to germ cells.

176 y expressed in the soma are mis-expressed in germ cells.

177 ne but to also involve epigenetic factors in germ cells.

178 f meiotic initiation in both male and female germ cells.

179 athogen-free mice to germ-free mice restored germ-dependent clonotypes, directly implicating BCR sele

180 e microbial and nutritional quality of wheat germ during 14 days of germination.

181 highly purified fraction of fermented wheat germ extract (FWGE), increases the carbon flux into the

182 How germ fate is induced in these animals, and whether this

183 effect of colonization using human donors in germ-free (GF) mice on the gut-liver-brain axis.

184 Here we demonstrate that co-housing of germ-free (GF) mice with specific-pathogen free (SPF) mi

185 rs have used to understand these effects are germ-free (GF) mouse models.

186 development of PLZF(+) innate lymphocytes in germ-free (GF) neonatal mice is restored by colonization

187 e proteolytic signature were investigated in germ-free adult mice and in dams colonized with HC, pre-

188 t radiation-induced damage and death in both germ-free and conventionally housed recipients.

189 of conventionally raised mice compared with germ-free animals.

190 performed studies with conventional C57/BL6, germ-free C57/BL6, Nestin-creER(T2):tdTomato, Nestin-GFP

191 lopment of intestinal carcinomas, even under germ-free conditions, and therefore does not involve mic

192 factor and interleukin-17A, and persisted in germ-free conditions.

193 Mcl1 (controls) were raised under normal or germ-free conditions.

194 both groups of colonized mice in addition to germ-free control mice.

195 Embryos from antibiotic-treated and germ-free dams exhibited reduced brain expression of gen

196 free than in SPF mice, and winner B cells in germ-free germinal centres are enriched in 'public' clon

197 Germ-free housing of Mcl1(DeltaIEC) mice reduced markers

198 diet, to induce cholestatic liver disease in germ-free mice and germ-free mice conventionalized with

199 ansferred from villin-TLR4 mice to wild-type germ-free mice caused increased H(2)O(2) production and

200 notypes and serum antibodies were reduced in germ-free mice compared with conventionally raised mice.

201 lestatic liver disease in germ-free mice and germ-free mice conventionalized with the microbiome from

202 ifferent order, or simultaneously into young germ-free mice fed human infant formula.

203 Colonic LMMP of germ-free mice given TLR2 agonist had increased neuronal

204 Here, we show in germ-free mice or in oral antibiotic-treated conventiona

205 f faeces from specific-pathogen-free mice to germ-free mice restored germ-dependent clonotypes, direc

206 s and human microbiome transplantations into germ-free mice revealed that the KD-associated gut micro

207 Young germ-free mice that had been fed a Bangladeshi diet were

208 cleverly colonized ex-germ-free mice to demonstrate that immune genes regulate

209 model of auxotrophic Salmonella infection in germ-free mice to show that live bacterial virulence fac

210 Skin of neonatal mice and adult germ-free mice was seeded with low numbers of antigen-pr

211 Germ-free mice were colonized as neonates with either a

212 Germ-free mice were given drinking water with TLR2 agoni

213 Colonization of germ-free mice with a defined microbial consortium (Olig

214 antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer

215 anscription factor cFos, and colonization of germ-free mice with bacteria that produce short-chain fa

216 Conversely, they were scarce in germ-free mice, but their presence was restored by gut r

217 Using antibiotic-treated or germ-free mice, we show that parathyroid hormone (PTH) o

218 rebellum, cortex and hippocampus relative to germ-free mice.

219 in tumor development was tested in wild-type germ-free mice.

220 lls of microbiota-replete mice compared with germ-free mice.

221 eased neuronal numbers compared with control germ-free mice.

222 studies of the effects of fecal transfer in germ-free mice.

223 ensity and decreased firing rate observed in germ-free mice.

224 colonize conventionally raised mice, but not germ-free or antibiotic-treated animals.

225 icient states as compared to control in both germ-free or E. coli gut microbiota states was used to q

226 iated germinal centres is markedly higher in germ-free than in SPF mice, and winner B cells in germ-f

227 The generation of axenic (germ-free) and gnotobiotic model systems has been vital

228 consortium (Oligo-MM(12)) does not eliminate germ-free-associated clonotypes, yet does induce a conco

229 Me31B is a protein component of Drosophila germ granules and plays an important role in germline de

230 Germ granules deliver mRNAs required for germline develo

231 binding protein Pur-alpha, as a component of germ granules in C. elegans We show that PLP-1 is essent

232 m marker Vas in the ovaries and early embryo germ granules.

233 form distinct aggregates in the vicinity of, germ granules.

234 otentially causal roles of genes involved in germ layer differentiation (WDHD1, DNM1L, TULP3), beta-c

235 embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues,

236 d study effects on embryonic stem cell (ESC) germ layer specification and mesodermal specification, u

237 In zebrafish, germ layer specification depends on the inheritance of m

238 To determine whether germ layer specification is robust to altered cell-to-ce

239 n stages for cell type specification of each germ layer.

240 Here, we show that germ-layer patterning in avian gastrulation is ipsilater

241 an development after implantation, including germ-layer specification and axial organization(1-3).

242 Cells from different germ layers - endoderm, mesoderm and ectoderm - can spon

243 king extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm an

244 During mammalian gastrulation, germ layers arise and are shaped into the body plan whil

245 ptomes of human extraembryonic and embryonic germ layers differentiated in a stereotyped arrangement.

246 fferentiate to form derivatives of the three germ layers organized spatiotemporally, without addition

247 the epiblast generates mesoderm and endoderm germ layers through epithelial-mesenchymal transition (E

248 orsal-ventral (DV) gradient across all three germ layers, including the hindgut.

249 ain approximately 20 cell types across all 3 germ layers, that inter-teratoma cell type heterogeneity

250 Among the three embryonic germ layers, the mesoderm plays a central role in the es

251 al origin, receiving contributions from both germ layers.

252 luripotency, before giving rise to the three germ layers.

253 effects of genetic perturbations across all germ layers.

254 In germ-like cells in vitro, both serum and PPAR agonist in

255 key quality control mechanism in the female germ line and a critical determinant of fertility and ge

256 comitant genetic inactivation of Bmp9 in the germ line and Bmp10 in the right atrium led to dramatic

257 lencing different types of transgenes in the germ line and for suppressing the expression of several

258 line, rcd4 mutants are fertile and have male germ line centrioles of normal length.

259 The germ line did show signs of postirradiation recovery in

260 Clonal animals do not sequester a germ line during embryogenesis.

261 he effect of the presence and absence of the germ line on somatic repair under benign and stressful c

262 ryo represent a lineage that converts to the germ line when the primordial germ cells are deleted.

263 We tested "the expensive germ line" hypothesis by generating germline-free zebraf

264 genotoxic stress affecting the soma and the germ line, and tested how fast the soma recovered follow

265 influence the epigenetic information of the germ line, disproportionately affecting genes with criti

266 r Ana3 for centriole development in the male germ line, rcd4 mutants are fertile and have male germ l

267 When targeted to the germ line, the same cascade progresses across animal gen

268 0 mum) and stain positive for Vasa, an early germ line-specific protein.

269 ly passed down or suppressed in the maternal germ line.

270 ction of UBIAD1 in mice; however, homozygous germ-line elimination of the Ubiad1 gene caused embryoni

271 re currently offered to women with high-risk germ-line mutations, the in vivo HSPC gene therapy appro

272 ntial maternal transcripts to the progenitor germ lineage for later translation.

273 ssion but is required to enrich mRNAs in the germ lineage for robust germline development.

274 P granule localization within the progenitor germ lineage.

275 ion of DNA methylation dynamics in mammalian germ lines and early embryos with a focus on both mice a

276 derstanding of DNA methylation regulation in germ lines and early stage embryos.

277 factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-

278 ormone on both the male and female ancestral germ lines.

279 Of course, this germ may also be resistant to other anti-tuberculosis dr

280 The expeller-pressed (EP) corn germ oil oleogels were prepared using rice bran wax (RBX

281 es that oleogels made by refined and EP corn germ oil together with RBX have the potential to imitate

282 contents this oil is surpassed only by wheat germ oil.

283 ld form oleogels in both refined and EP corn germ oils at a concentration >=3 wt%.

284 Whereas oskar mRNA is essential for germ plasm assembly during oogenesis, we show that it is

285 is likely independent of the Osk-Vas-Tud-Aub germ plasm assembly pathway although its proper enrichme

286 les, P-bodies/sponge bodies, and possibly in germ plasm granules.

287 inally, we show that Me31B's localization in germ plasm is likely independent of the Osk-Vas-Tud-Aub

288 Me31B likely colocalizes and interacts with germ plasm marker Vas in the ovaries and early embryo ge

289 athway although its proper enrichment in the germ plasm may still rely on certain conserved germ plas

290 ules, contains oskar mRNA, which encodes the germ plasm organizer.

291 nd cytoskeleton/motor proteins, but the core germ plasm proteins Vas, Tud, and Aub were significantly

292 rm plasm may still rely on certain conserved germ plasm proteins.

293 least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the

294 f P granules, RNA granules in the C. elegans germ plasm.

295 Reproductive division of labor (e.g. germ-soma specialization) is a hallmark of the evolution

296 e canonical C. elegans distal tip cell (DTC) germ stem cell niche mediated by previously unobserved,

297 e hunting, beginning with the history of pre-germ theory epidemiological studies, through the microbi

298 aled highly suppressed conidial germination, germ tube development, appressoria formation and mycelia

299 as they produced smaller sporangia, shorter germ tubes, and fewer appressoria.

300 s on the lipolytic enzymes activity in wheat germ were investigated.