ライフサイエンスコーパス: germ cell

1 xpressed on GSCs and differentiating vasa(+) germ cells.

2 eding epigenetic reprogramming in primordial germ cells.

3 ns predominantly as a regulator of Dnmt3a in germ cells.

4 gnals in the periphery that communicate with germ cells.

5 y altered chromatin structure in postmeiotic germ cells.

6 ession of Tfap2 in i-cells converted them to germ cells.

7 y expressed in the soma are mis-expressed in germ cells.

8 ne but to also involve epigenetic factors in germ cells.

9 f meiotic initiation in both male and female germ cells.

10 te into the gonad primordia, and mature into germ cells.

11 nerations via epigenetic modification of the germ cells.

12 ates the aerial part of plants including the germ cells.

13 olic diseases on the function of stem and/or germ cells.

14 complex that localizes to the nucleoplasm of germ cells.

15 intercellular bridges connecting developing germ cells.

16 r and silence ZAM expression specifically in germ cells.

17 s the most abundant CL species in testicular germ cells.

18 with repressive epigenetic pathways in male germ cells.

19 ic protein GCNA in the genome maintenance of germ cells.

20 from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo

21 -protein diet elevates ROS in the testicular germ cells, altering ATF7 activity and H3K9me2 abundance

22 ated multi-organ in vitro systems to support germ cell and embryo function, and to display characteri

23 perates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT s

24 as carriers of epigenetic information during germ cell and pre-implantation development by ensuring t

25 llected during multiple stages of primordial germ cell and pre-implantation development, we find that

26 Drosophila ovary is a widely used model for germ cell and somatic tissue biology.

27 l CpG methylation drops to 10% in primordial germ cells and 20% in the inner cell mass of the blastoc

28 ied 5 distinct cell clusters associated with germ cells and 6 with granulosa cells.

29 Its normal expression is restricted to germ cells and attenuation of its activity results in al

30 play a significant and multifaceted role in germ cells and development.

31 functions of phase-separated compartments in germ cells and examine the various ways in which phase s

32 TEX15 is expressed in embryonic germ cells and functions during genome-wide epigenetic r

33 bryonic sac, and specification of primordial germ cells and gastrulating cells (or mesendoderm cells)

34 tinia symbiolongicarpus Tfap2 mutants lacked germ cells and gonads.

35 each specific stage, and the interactions of germ cells and granulosa cells basing on known and novel

36 f the biological process most represented in germ cells and granulosa cells or common to both cell ty

37 e previously reported expression of genes by germ cells and granulosa cells, our analyses identified

38 ff between the repair and maintenance of the germ cells and growth and maintenance of the soma may ex

39 sters on the Y chromosome, expressed in male germ cells and possibly associated with sperm motility.

40 bryonic sac, and specification of primordial germ cells and primitive streak cells.

41 regulates retrotransposon silencing in male germ cells and provides a molecular link between DNA met

42 lausible because KLOTHO is expressed in both germ cells and spermatozoa and forms with FGFR1 a specif

43 LOTHO and FGFR1 were also expressed in human germ cells and spermatozoa, and FGF23 treatment augmente

44 s, one we conclude represents the primordial germ cells and the other state is transiently present du

45 diated deletion of W38 in chicken primordial germ cells and the successful production of the gene-edi

46 e novo mutations examined pedigrees (and not germ cells) and thus were likely affected by selection.

47 er, further differentiated cells (primordial germ cells), and their analysis using cell biological an

48 rophase state (leptotene/zygotene) in mutant germ cells, and identified several misregulated genes in

49 In mouse fetal testes, the majority of germ cell apoptosis coincides with the onset of male dif

50 of chromatoid bodies of round spermatids and germ cell apoptosis.

51 onverts to the germ line when the primordial germ cells are deleted.

52 Many germ cells are eliminated during development, long befor

53 In Drosophila, male germ cells are intimately associated and co-differentiat

54 initiation in mice, and the majority of male germ cells are lost in the meiotic defect of first wave

55 jority of genes under PHF7 control in female germ cells are not under PHF7 control in male germ cells

56 Male germ cells are sensitive to heat stress and testes must

57 Germ cells are vulnerable to stress.

58 628 in the mouse and uncovered a postmeiotic germ cell arrest at the round spermatid stage in the sem

59 nd that each of the proteins was detected in germ cells at the appropriate stages to regulate Tnp RNA

60 f mitochondrial dynamics in regulating early germ cell behavior is relatively less-well understood.

61 esource to understand many questions in both germ cell biology and stem cell biology fields.

62 re, we use our current understanding of male germ cell biology and TLR function as a starting point t

63 l for DNMT3A-mediated de novo methylation in germ cells but is dispensable for de novo methylation du

64 ted a similar heat shock response to control germ cells, but could not maintain that response.

65 assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein

66 demonstrate how niche cell wrapping protects germ cells by manipulating their signaling environment a

67 to alter chromatin in soon-to-be fertilized germ cells by recruiting the histone chaperone FACT, dis

68 piRNAs protect fetal germ cells by targeted mRNA destruction and deposition o

69 r the specialized maturation of haploid male germ cells called spermiogenesis.

70 recipient embryo and the development of the germ cells can be carried out in well-defined laboratory

71 Germ cells carry genetic information to the next generat

72 Conditional loss of UHRF1 in postnatal germ cells causes DNA hypomethylation, upregulation of r

73 Germ cells clustered into six meiotic substages, as well

74 he transcriptional profiles of 19 363 single germ cells collected from E12.5, E14.5, and E16.5 mouse

75 Therefore, Tfap2 is a regulator of germ cell commitment across germ line-sequestering and g

76 Animal germ cells communicate directly with each other during g

77 Finally, distinct differentiating germ cell cyst samples do not exhibit obvious dosage com

78 to other sites of open chromatin, leading to germ cell death and sterility.

79 development but leads to reduced numbers of germ cells, delayed meiosis and reduced fecundity in adu

80 How germ cells develop, function, and eventually give rise t

81 nt in parental chromosomes during primordial germ cell development and after fertilization.

82 of spermatogenesis, we both precisely stage germ cell development and enrich for rare somatic cell-t

83 ture targeted mechanistic studies of primate germ cell development and in vitro gametogenesis.

84 as a multifunctional hub for haematopoiesis, germ cell development and nutritional supply.

85 ight the importance to understand primordial germ cell development and the timing of gametogenesis wi

86 proteins to silence transposons and promote germ cell development in animals.

87 Piwi proteins are important for germ cell development in most animals.

88 s to understand the mechanisms that regulate germ cell development opens promising new avenues to dev

89 piRNA pathway to drive piRNA biogenesis and germ cell development.

90 cytokines, and other biomolecules to support germ cell development.

91 Lineage tracing confirmed that germ cells die as clones independent of intercellular br

92 provide more insights into the mechanisms of germ cell differentiation after birth and potentially ex

93 IR of female PGCs caused uncoupling of germ cell differentiation and meiotic initiation, while

94 s retinoic acid (RA) and plays a key role in germ cell differentiation by controlling local distribut

95 (SOX9), which ensure that RA is degraded and germ cell differentiation is blocked.

96 DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from har

97 1 and ADAD2 are essential regulators of male germ cell differentiation with molecular functions unrel

98 EGFR activation contributed to premature germ cell differentiation.

99 D1 and ADAD2, on testis RNA editing and male germ cell differentiation.

100 ation in vitro, and to monitor PT growth and germ cell division.

101 stic increase in the ratio of male to female germ cell divisions after the onset of spermatogenesis,

102 A disproportionate number of germ cell divisions were observed at the DTC-Sh1 interfa

103 , suggesting that epigenetic modification of germ cell DNA may mediate transgenerational transmission

104 to examine 3D chromatin organization in male germ cells during spermatogenesis.

105 Cas9 restriction to male germ cells elicits autonomous double-strand-break repair

106 experience before conception have implicated germ cell epigenetic programming.

107 pports our hypothesis that earlier errors in germ-cell epigenetic reprogramming derail differentiatio

108 e formed without specification of primordial germ cells, epigenetic reprogramming or meiosis, and dem

109 uman cohorts to identify changes in paternal germ cell epigenetics in association with offspring dise

110 cks2(-/-) germ cells express significantly reduced levels of the M

111 proteins in Drosophila melanogaster known as germ cell-expressed (Gce) and its duplicate paralog, met

112 script profile of two cell states expressing germ cell factors, one we conclude represents the primor

113 that genetic rescue of DNA repair-deficient germ cells (Fancm(-/-) ) leads to increased mutation inc

114 t adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbi

115 with P granule exit for two mRNAs coding for germ cell fate regulators.

116 d that PRDM14 might be dispensable for human germ cell fate.

117 Our results indicate that the fetal germ-cell fate is based on discrete cell-heritable ident

118 We studied fetal germ-cell fates and discovered that both apoptosis and d

119 a-linolenic acid (DGLA; 20:3n-6) can trigger germ-cell ferroptosis and sterility in the nematode Caen

120 For example, in Drosophila female germ cells, forced expression of the testis-specific PHD

121 s evolved to fulfil a crucial role in insect germ cell formation.

122 ell resulting from a reprogrammed primordial germ cell from the thymus.

123 i glycoprotein MGAT4D as a protector of male germ cells from heat stress.

124 AT4D is a novel, intrinsic protector of male germ cells from heat stress.

125 pient) as an appropriate host for primordial germ cells from native poultry breeds.

126 their signaling environment and by shielding germ cells from unwanted cellular interactions that can

127 This work shows that GCNA protects germ cells from various sources of damage, providing ins

128 The transcriptome of germ cells from vitamin-C-deficient embryos is remarkabl

129 LDH) present in both Sertoli cells (SCs) and germ cells (GCs).

130 RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon

131 ation, chromosome segregation, and repair of germ cell genomes remain incompletely understood.

132 ogenesis is the process by which uncommitted germ cells give rise to haploid sperm.

133 amined whether XCD extends to human prenatal germ cells given their similarities to naive pluripotent

134 o complete meiotic arrest and elimination of germ cells; however, the mechanisms underlying this arre

135 We found that female human primordial germ cells (hPGCs) display reduced X-linked gene express

136 -cell lineage originates as human primordial germ cells (hPGCs).

137 ignaling pathways acting between somatic and germ cells in a stage-specific manner during the perinat

138 ification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebr

139 Developing germ cells in Drosophila have an additional specialized

140 tant for regulating the maintenance of early germ cells in larval testes.

141 romatin states transmitted to the primordial germ cells in offspring influence germline transcription

142 otransposons must be activated in developing germ cells in order to survive and propagate, how they a

143 HLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation drama

144 ts that reduce coupling between the soma and germ cells in the Caenorhabditis elegans gonad.

145 anes, P granules (associated with progenitor germ cells in the P lineage) and P-bodies (associated wi

146 ids maintained wild-type array of premeiotic germ cells in the testes, but in them harmful Stellate g

147 2 or 12-Lox activity also inhibits homing of germ cells in vivo Using a live-imaging chemotaxis assay

148 Here, we show that PHF7-expressing ovarian germ cells inappropriately express hundreds of genes, ma

149 ntricate crosstalk between Sertoli cells and germ cells including spermatogonia, spermatocytes, haplo

150 type cells rescued gonad development but not germ cell induction in Tfap2 mutants.

151 ormative stem (FS) cells respond directly to germ cell induction.

152 However, no germ cell intrinsic mechanism that protects from heat ha

153 cytological and scRNAseq analyses identified germ-cell intrinsic and extrinsic genes responsive to Se

154 Repression of cellular reprogramming in germ cells is critical to maintaining cell fate and fert

155 rones could be involved in repressing TEs in germ cells is still unknown.

156 Germ cells lacking Mgat4d generally mounted a similar he

157 is study reveals that ectopic PHF7 in female germ cells leads to a loss of sexual identity and the pr

158 d capable of differentiation into primordial germ cell-like cells.

159 eloped a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis us

160 The human germ-cell lineage originates as human primordial germ ce

161 t does not assemble P granules in primordial germ cells loses competence for RNA-interference over se

162 ype of the Dazl knockout mouse has extensive germ cell loss because of incomplete meiosis.

163 There was no significant early germ cell loss or meiotic delay.

164 ructure, blocks differentiation and promotes germ cell loss, phenotypes that are partially rescued by

165 tion activates the NL checkpoint that causes germ cell loss.

166 s into how mitochondrial dynamics can impact germ cell maintenance and differentiation via distinct m

167 s (patients with clinically confirmed active germ cell malignancy [aGCM]) and controls (patients with

168 Germ cells manifest a unique gene expression program and

169 lure of the maintenance and/or activation of germ cell markers and pluripotency genes.

170 als involved in cellular function, including germ cell maturation.

171 Integration also occurs in germ cells, meaning that the virus can be inherited and

172 It has been shown that Wdr62 is required for germ cell meiosis initiation in mice, and the majority o

173 also demonstrated a new function of WDR62 in germ cell meiosis, through its interaction with CEP170.

174 ing protein Dnd1 (Dnd1(Ter/Ter) ), many male germ cells (MGCs) fail to enter G1/G0 and instead form t

175 ygous embryos (Mdr49/Npc1a) display enhanced germ cell migration defects when compared with single mu

176 c HMG Coenzyme A reductase (Hmgcr) to induce germ cell migration defects.

177 rm-specific inactivation of Npc1a results in germ cell migration defects.

178 Here we demonstrate, using primordial germ cell migration in mouse as a developmental model, t

179 ratory processes, including inflammation and germ cell migration, means that self-generated gradients

180 When germ cells mis-express somatic genes they can be directl

181 4 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripo

182 were identified on a per genome basis, with germ cell neoplasia in situ possessing the least (median

183 ypomorph ovaries had a phenotype of impaired germ cell nest breakdown with a 66% reduction in total f

184 tial for normal intercellular bridges within germ cell nests and their timely breakdown, with a major

185 a lesser extent in Mgat4d[-/-] heat-stressed germ cells (NFkappaB response, TNF and TGFbeta signaling

186 c core (the female medulla), overlain by the germ cell niche (the cortex).

187 interpretation of Hedgehog signalling in the germ cell niche.

188 ectopic niche similar to the native gonadal germ cell niche.

189 We identified Germ Cell Nuclear Acidic Peptidase (GCNA) as a conserved

190 echanisms that maintain nuclear integrity as germ cell nuclei progress through meiotic development an

191 an active piRNA pathway and TE repression in germ cells of human fetal testis.

192 now report that de novo methylation of male germ cells of mice involves the transient opening of het

193 U-box, promoting either RNA stabilization in germ cells or degradation in the soma.

194 1-ORF1p expression is heterogeneous in fetal germ cells, peaks at mid-gestation and declines concomit

195 Smad downstream effectors during primordial germ cell (PGC) development.

196 s repair process is essential for primordial germ cell (PGC) maturation during embryonic development.

197 Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilate

198 tion and direct responsiveness to primordial germ cell (PGC) specification, a unique functional featu

199 onial specification whereby human primordial germ cell (PGC)-like cells differentiated from human ind

200 ring embryonic gonad coalescence, primordial germ cells (PGCs) follow a carefully choreographed migra

201 we investigated niche wrapping of primordial germ cells (PGCs) in the C. elegans embryonic gonad prim

202 t of mesodermal cells to form the primordial germ cells (PGCs) is restricted to the second through th

203 Caenorhabditis elegans primordial germ cells (PGCs) jettison mitochondria and cytoplasm by

204 opulation arises from pluripotent primordial germ cells (PGCs) that enter the fetal testis around emb

205 These primordial germ cells (PGCs) undergo rapid proliferation, yet the g

206 the functional role of piwil2 in primordial germ cells (PGCs) was investigated in Nile tilapia using

207 ocesses: isolation and culture of primordial germ cells (PGCs), modification of the genome of PGCs in

208 Primordial germ cells (PGCs), the founder cells of the germline, ar

209 l migration program of Drosophila primordial germ cells (PGCs), we show that cluster dispersal is acc

210 ection of plasmids in circulating Primordial Germ Cells (PGCs).

211 ripotency to the specification of primordial germ cells (PGCs).

212 By E15.5, the Sertoli cell and germ cell population declined in SC-SF-1(-/-) mice resul

213 iosis results in heterogeneity in the female germ cell populations, which limits the studies of meios

214 ive-cell imaging and discovered that ectopic germ cells preferentially induce body-wall muscle to ext

215 Blood and germ cell progenitor development is affected similarly.

216 for germline development to pole cells, the germ cell progenitors.

217 erse environmental factors able to influence germ cell programming and potentially impact offspring d

218 'intersex' or male phenotype may compromise germ cell progression into meiosis, causing cortical ger

219 n Sertoli cells is known to be important for germ-cell progression through meiosis, but the extent to

220 2/3 perturbed the DTC-Sh1 interface, reduced germ cell proliferation, and shifted a differentiation m

221 t non-apoptotic roles for CED-3 in promoting germ cell proliferation, meiotic chromosome disjunction,

222 cient CRISPR/Cas9 gene editing in primordial germ cells represents a substantial addition to genotech

223 Identification of new pathways governing germ cell reprogramming is critical to understanding how

224 f alms1a in GSCs, but not in differentiating germ cells, results in rapid loss of centrosomes due to

225 l and electron microscopy of HIPK4-null male germ cells reveals defects in the filamentous actin (F-a

226 o assess the impact of Adad mutation on male germ cell RNA editing, CRISPR-induced alleles of each we

227 a role for both in meiotic and post-meiotic germ cell RNA editing.

228 We demonstrate that a testicular germ-cell-secreted epidermal growth factor-like protein,

229 Maintenance of germ cell sexual identity is essential for reproduction.

230 rnover during spermatid differentiation, and germ cell-soma communication.

231 We show that CSMD1 is enriched at the germ-cell/somatic-cell interface in both male and female

232 Here, we identify the germ cell specific Golgi glycoprotein MGAT4D as a protec

233 We show that germ cell-specific BAF knockdown causes phenotypes that

234 Mice with germ cell-specific Klotho (gcKL) deficiency neither had

235 oles in meiosis by generating the first male germ cell-specific Phb-cKO mouse.

236 t al., 2020) reveal an essential function of germ cell-specific protein GCNA in the genome maintenanc

237 stulated as likely candidates for sustaining germ cell-specific transcription programs throughout the

238 We further generated germ-cell-specific, conditional knockout mice for the ke

239 ~3% of DNMs originated following primordial germ cell specification in a parent, and differed from n

240 ns during a developmental trajectory towards germ cell specification, and establish a paradigm for st

241 Germ cells specified during fetal development form the f

242 targeting RNA binding proteins in subsets of germ cells-suggest that this new mechanism for controlli

243 erm cells are not under PHF7 control in male germ cells, suggesting that PHF7 is acting in a tissue-s

244 rence of metachronous contralateral (second) germ cell testicular cancer (TC).

245 e incidence may be much higher in developing germ cells than in zygotes.

246 ermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis

247 ed transcriptional heterogeneity among fetal germ cells that included an apoptosis-susceptible popula

248 to extend cellular processes that enwrap the germ cells, the extent of which was strikingly similar t

249 ing rate, the sex ratio, the presence of own germ cells, the fertility and the phenotype of viable hy

250 Due to the loss of Sertoli and germ cells, the testis weights of SC-SF-1(-/-) mice at 6

251 ost-zygotic, and present in both somatic and germ cells; these gonosomal mutations occurred at equiva

252 (tdnd) and used it as a molecular marker for germ cells to obtain basic information essential for tra

253 s coupled to transcription response times of germ cells to protect future offspring.

254 agation of species depends on the ability of germ cells to protect their genome from numerous exogeno

255 l progression into meiosis, causing cortical germ cells to remain in an immature state in the embryo.

256 n facilitating crosstalk between Sertoli and germ cells to support spermatogenesis and thus fertility

257 es are biomolecular condensates that promote germ cell totipotency in animals.

258 of cellular differentiation from primordial germ cells toward meiocytes.

259 Germ-cell transcription factors control gene networks th

260 al neurons, FACT is not recruited by HSF1 in germ cells, transcription occurs but is delayed, and pro

261 The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the sam

262 st, ectoderm, mesoderm, endoderm, primordial germ cells, trophectoderm, and amnion.

263 atoma in patients with metastatic testicular germ cell tumor (GCT) is of unknown prognostic significa

264 90 to 0.96), was lower after nonseminomatous germ cell tumor (HR, 0.58; 95% CI, 0.35 to 0.96) and dec

265 Patients with testicular germ cell tumor (TGCT) are at increased risk of developi

266 a +/- necrosis (20%), viable nonteratomatous germ cell tumor +/- teratoma (41%), and secondary somati

267 oogenesis, leading to either an agametic or germ cell tumor phenotype.

268 iectomy, and pathology showed a 1.5-cm mixed germ cell tumor with 85% embryonal, 10% yolk sac tumor,

269 Germ cell tumors (GCTs) are the most common cancer in me

270 Some germ cell tumors (GCTs) in men develop into hematologic

271 e management of central nervous system (CNS) germ cell tumors (GCTs).

272 iomarkers to differentiate non-germinomatous germ cell tumors (NGGCTs) from germinomas are critical,

273 eillance (AS) for testicular nonseminomatous germ cell tumors (NSGCT) is widely used.

274 th pathologic stage (PS) IIA nonseminomatous germ cell tumors (NSGCTs) is 10%-20% but increases to >=

275 ry networks in two major types of testicular germ cell tumors (TGCT): seminoma (SE) and non-seminoma

276 Testicular germ cell tumors (TGCTs) are classified into two main su

277 y associated with lymphomas and some ovarian germ cell tumors, we present a case of calcitriol overpr

278 ights into how cell identity is lost in some germ cell tumors.

279 arcoma, mesothelioma, melanoma, gastric, and germ cell tumors.

280 bility in flies, worms, zebrafish, and human germ cell tumors.

281 reproductive system, including proliferative germ-cell tumors and uterine masses that express neurona

282 While most testicular germ cell tumours (TGCTs) exhibit exquisite sensitivity

283 ovarian cancers, including malignant ovarian germ cell tumours, sex cord-stromal tumours, and small c

284 Post-pubertal testicular germ-cell tumours (TGCTs) can present with a variety of

285 ong-term mitofusin loss, all differentiating germ cell types are depleted, but proliferation of stem-

286 laying severe teratospermia and Adad2 mutant germ cells unable to progress beyond round spermatid.

287 Most notably, although Prdm9(-/-) germ cells undergo cytological arrest in a late-leptoten

288 ng transcription factors expressed in female germ cells, we analyzed global gene expression profiles

289 However, most germ cells were arrested at metaphase of meiosis I and n

290 ing spermatogenesis was not affected and the germ cells were gradually repopulated at later developme

291 nd 1 branch point of fate transition for the germ cells were revealed, as well as for the granulosa c

292 A helicases expressed in meiotic and haploid germ cells which plays an essential role in spermatogene

293 ure of the subsequent DNA repair response in germ cells, which ensures faithful transmission of the g

294 ranscriptome features of peri-meiotic female germ cells, which offers new information not only on mei

295 sed in tissues, others are expressed in only germ cells with aberrant reactivation in multiple cancer

296 Conversely, the earliest germ cells with high levels of L1-ORF1p express low leve

297 ess of the testis (43 degrees C for 25 min), germ cells with inactivated Mgat4d were markedly more se

298 lian spermatogenesis is sustained by mitotic germ cells with self-renewal potential known as undiffer

299 s whose expression is normally restricted to germ cells yet aberrantly activated in tumors, where the

300 pacity to undergo meiosis defines vertebrate germ cells, yet mechanisms driving initiation of this sp