ライフサイエンスコーパス: germ layer

1 e of progeny to differentiate into all three germ layers).

2 n stages for cell type specification of each germ layer.

3 of blood lineage formation from the mesoderm germ layer.

4 differentiate into cells from more than one germ layer.

5 em cell niches, or signal inductively across germ layers.

6 atomas, and can differentiate into all three germ layers.

7 tiation propensities of hPSCs into all three germ layers.

8 al origin, receiving contributions from both germ layers.

9 entiation of pluripotent epiblast cells into germ layers.

10 differentiation that gives rise to all three germ layers.

11 c expression in the derivatives of all three germ layers.

12 lerate mouse ESC formation of cells of three germ layers.

13 iation of mouse ESCs into cells of all three germ layers.

14 en broadly attributed to the three embryonic germ layers.

15 necessary for differentiation into all three germ layers.

16 d differentiate into derivatives of multiple germ layers.

17 n different contexts to pattern the emerging germ layers.

18 le in regulating the separation of embryonic germ layers.

19 luripotency, to differentiate into all three germ layers.

20 differentiate into derivatives of all three germ layers.

21 SCs representing each of the three embryonic germ layers.

22 e levels of specific markers of the distinct germ layers.

23 l step toward differentiation into all three germ layers.

24 ntiate, in vitro and in vivo, into different germ layers.

25 generated teratomas consisting of the three germ layers.

26 encompassed derivatives of the three primary germ layers.

27 as distantly related as cells from different germ layers.

28 as in vivo, and differentiate into all three germ layers.

29 otency, the capacity to specify cells of all germ layers.

30 ation of bona fide tissue progenitors of all germ layers.

31 changes, and formed teratomas with all three germ layers.

32 ent and are able to form all three embryonic germ layers.

33 te-specific structure derived from all three germ layers.

34 contribute to specific portions of different germ layers.

35 entiate into teratomas composed of the three germ layers.

36 including lineages from all three embryonic germ layers.

37 et of gastrulation affect the subdivision of germ layers.

38 milar ontogenies, but originate in different germ layers.

39 delays or enhances differentiation into the germ layers.

40 gastrulation, cells separate into different germ layers.

41 differentiate into derivatives of all three germ layers.

42 rentiation into cells representing all three germ layers.

43 erentiate into all the lineages of the three germ layers.

44 ignaling centers that induce and pattern the germ layers.

45 to advanced derivatives of all three primary germ layers.

46 and the organization of all three embryonic germ layers.

47 at results in the formation of three primary germ layers.

48 for a hierarchical emergence of the primary germ layers.

49 nteraction of tissues derived from all three germ layers.

50 ects in morphogenesis of all three embryonic germ layers.

51 SCs efficiently differentiate into all three germ layers.

52 differentiation of human ESCs into all three germ layers.

53 the ability to differentiate into all three germ layers.

54 luripotency, before giving rise to the three germ layers.

55 effects of genetic perturbations across all germ layers.

56 gramming, and differentiation into the three germ layers.

57 n of human embryonic stem cells to the three germ layers.

58 ifferentiation potential of hPSCs across all germ layers.

59 tency and commit to multiple lineages in all germ-layers.

60 erentiated cells representative of all three germ layers, a definitive test of pluripotency.

61 ealed that cells in different regions of the germ layers acquire location-specific cell fates during

62 gate the underlying mechanisms of scaling of germ layers across Drosophila species, we quantified the

63 Despite their origins in different germ layers, all of these insulin-producing cells share

64 e and induced stem cell markers generating 3-germ layers, all qualifiers of acquired pluripotency.

65 la species, which result in unequally scaled germ layers along the dorso-ventral axis and the reposit

66 Specification of germ layers along the dorsoventral axis by morphogenetic

67 ble to differentiate into cells of the three germ layers, although after SPRY2 KD there was a tendenc

68 distribution of maternal transcripts for the germ layer and dorsal/ventral determinants VegT and Wnt1

69 can be used to track the development of this germ layer and its specification to a hepatic fate.

70 ptome of small groups of cells from a single germ layer and to retain spatial information, dorsal and

71 tive cell types spanning the three embryonic germ layers and assessed their immunogenicity in vitro a

72 o derivatives of all three primary embryonic germ layers and can self-renew indefinitely.

73 UCSFB lines formed derivatives of the three germ layers and CDX2-positive progeny, from which we der

74 25+ MASCs generated derivatives of the three germ layers and contributed to chimaeric embryos, with c

75 ue interactions between derivates of all the germ layers and coordinated morphogenetic movements in t

76 with the animal cap, mix with cells of other germ layers and differentiate according to their new pos

77 This mechanism spatially separates the germ layers and establishes the organizational foundatio

78 Expanded Nanog null cells colonize embryonic germ layers and exhibit multilineage differentiation bot

79 iate into gastruloids, expressing markers of germ layers and extraembryonic cells in radial arrangeme

80 king extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm an

81 d with the factors initially responsible for germ layers and organizer formation, including Nodal its

82 e the embryonic arrangement of the mammalian germ layers and provide an assay to assess the structura

83 Gastrulation movements form the germ layers and shape them into the vertebrate body.

84 cation, leading to the formation of distinct germ layers and specialized tissues.

85 n the amphipod Parhyale hawaiensis all three germ layers and the germ line are determined by the eigh

86 n, the relative mRNA expression in the three germ layers and the trophoblast was abnormal in the EBs

87 tions from epithelial tissues from all three germ layers and therefore may be broadly applicable for

88 ty of GD hiPSC to differentiate to all three germ layers and to form teratomas in vivo.

89 with specific loss of DNA methylation in one germ layer, and in many cases a reciprocal gain in the o

90 genes in segmentation is restricted to this germ layer, and that mesoderm segmentation is either dep

91 s differentiate into cell types of all three germ layers, and a pluripotent gene expression program i

92 opment, involves contributions from multiple germ layers, and is controlled by many genes.

93 ferentiation of ESCs into cells of all three germ layers, and it is from these differentiating aggreg

94 ce, regulatory elements associated with each germ layer are either epigenetically primed or remodelle

95 he mid-tailbud stage, days after the somatic germ layers are established.

96 interact is crucial to understanding how the germ layers are established.

97 Concurrently, germ layers are formed and cell lineages are specified,

98 Inductive signals across germ layers are important for the development of the end

99 During mammalian gastrulation, germ layers arise and are shaped into the body plan whil

100 onset of gastrulation but then segregate by germ layer as gastrulation proceeds.

101 on, we initiate rapid emergence of all three germ layers as a complex function of GATA6 expression le

102 e and abundance depending on the neighboring germ layer, as well as the region of the embryo.

103 eriorly and medially within the plane of the germ layers at the transition from mid- to late gastrula

104 dhesion, which limits cell mixing as primary germ layers become specified.

105 l to form derivatives of all three embryonic germ layers both in vitro and in teratomas.

106 matic fates representing all three embryonic germ layers both in vitro and in vivo, despite a persist

107 n a germ layer (NSM transfating) or across a germ layer boundary (endoderm transfating).

108 Specification of the three germ layers by graded Nodal signaling has long been seen

109 ratomas, tumors consisting cells of multiple germ layers; by contrast, these tumors have never been o

110 itors normally fated to enter the mesodermal germ layer can be redirected towards the neural lineage.

111 ) cells to form cells and tissues from all 3 germ layers can be exploited to generate cells that can

112 y-active somatic cells derived from separate germ layers can be interconverted.

113 ement profile, and differentiated into all 3 germ layer cell types.

114 iferating populations, and the involution of germ layer cells induced by a diffusing morphogen during

115 ficient in HS, to differentiate into primary germ layer cells.

116 ng a tissue that originates from a different germ layer compared with blood, demonstrates that the aD

117 ectoderm producing blastomeres display intra-germ layer compensation.

118 haracteristic of all the three developmental germ layers, confirming their EB identity.

119 The endoderm germ layer contributes to the respiratory and gastrointe

120 e concerted movement of cells from different germ layers contributes to morphogenesis during early em

121 hat dynamic local Wnt signaling cues specify germ layer contribution and mesodermal tissue type speci

122 gastrulation stages, cells of the mesodermal germ layer converge slowly; during segmentation stages,

123 he posterior wall of the tailbud that make a germ layer decision after gastrulation to form spinal co

124 fter gastrulation and continue to make basic germ layer decisions.

125 have the potential to differentiate into all germ layer derivatives and may also be important for any

126 egulation of genes associated with all three germ layers despite maintaining pluripotency markers.

127 , supports a role of Hippo-Yap signalling in germ-layer development and reveals the contribution of v

128 ptomes of human extraembryonic and embryonic germ layers differentiated in a stereotyped arrangement.

129 otentially causal roles of genes involved in germ layer differentiation (WDHD1, DNM1L, TULP3), beta-c

130 gulates multiple embryonic events, including germ layer differentiation and morphogenesis; the cellul

131 single-cell RNA sequencing dataset on human germ-layer differentiation demonstrates how PHATE reveal

132 ssential for morphogenesis of the mesodermal germ layer during gastrulation.

133 cell populations at defined positions in the germ layers during development from pre- to late-gastrul

134 st stage embryos, the formation of the three germ layers during gastrulation and the differentiation

135 Formation of the three primary germ layers during gastrulation is an essential step in

136 ecification of the mesodermal and endodermal germ layers during gastrulation.

137 tions are crucial in spatial organization of germ layers during mammalian gastrulation.

138 erentiation into cell types derived of all 3 germ layers during teratoma formation.

139 city to differentiate into the three primary germ layers, ectoderm, mesoderm and endoderm, from which

140 The three germ layers--ectoderm, mesoderm, and endoderm--are affec

141 Pa results in self-organization of all three germ layers: ectoderm on the outside layer, mesoderm in

142 o the derivatives of all the three embryonic germ layers: ectoderm, mesoderm and endoderm.

143 rentiate into functional derivatives of each germ layer, ectodermal, endodermal, and mesodermal.

144 Cells from different germ layers - endoderm, mesoderm and ectoderm - can spon

145 uripotent epiblast self-organizes into the 3 germ layers-endoderm, mesoderm and ectoderm, which event

146 ic stem cells differentiating into the three germ layers: endoderm, mesoderm and ectoderm.

147 brafish that local posterior signals specify germ layer fate in two basal tailbud midline progenitor

148 that maintain ESC identity, also orchestrate germ layer fate selection.

149 growth factors and other signals that govern germ layer fate.

150 ave the pluripotent state and choose between germ layer fates.

151 tiate into advanced derivatives of all three germ layers, features very useful for understanding the

152 investigated cellular rearrangements during germ layer formation at the onset of gastrulation.

153 rs Pou5f3 and Sox3 determines competence for germ layer formation by extensively remodelling compacte

154 of cell adhesion and cortical tension during germ layer formation in zebrafish.

155 mechanisms responsible for the regulation of germ layer formation.

156 Germ-layer formation during gastrulation is both a funda

157 b-group proteins, which coordinate embryonic germ-layer formation in response to extraembryonic cues.

158 Overall, these results lead to a model of germ-layer formation in which, upon N-cadherin expressio

159 layer, indicating its critical role in early germ-layer formation.

160 avoidance as an unexplored mechanism driving germ-layer formation.

161 tes that the embryo's regulative response to germ layer founder loss, in the form of altered cell beh

162 we present a method of generating organized germ layers from a single mouse embryonic stem cell cult

163 rentiation of mature cell types of all three germ layers from pluripotent cells.

164 s and differentiation events, the endodermal germ layer gives rise to the epithelial lining of the di

165 onic blastomeres can contribute to different germ layers has never been fully explained.

166 rived organoids with components of all three germ layers have been generated, resulting in the establ

167 ge-specific stem/progenitor cells of another germ layer in one step, bypassing the intermediate pluri

168 Embryos allocate cells to the three germ layers in a spatially ordered sequence.

169 -1-60, give rise to derivatives of the three germ layers in a teratoma assay, and are karyotypically

170 nderstand the relationship between segmented germ layers in arthropods, we asked whether segmentation

171 g, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell

172 atomas, and contributed to cell types of all germ layers in chimeric animals.

173 ic stem cells (hESCs) can generate the three germ layers in culture; however, differentiation is typi

174 to ES cells and formed teratomas with three germ layers in nonobese diabetic/severely compromised im

175 e and female ES cells gave rise to all three germ layers in teratoma assays, though sex-specific diff

176 ed to allow for the development of the three germ layers in the developing embryo.

177 Amphioxus Gbx is expressed in all germ layers in the posterior 75% of the embryo, and in t

178 migration contribute to the establishment of germ layers in vertebrates and other animals, but a comp

179 fferentiated into cells of the three primary germ layers in vitro and also can generate chimeric mice

180 s, and impaired differentiation to all three germ layers in vitro and in vivo.

181 ifferentiating into derivatives of the three germ layers in vitro and into neurons and muscle fibers

182 re capable of differentiation into all three germ layers in vitro.

183 lling pathway patterns the embryo into three germ layers, in part by inducing the expression of no ta

184 cted differentiation into cells of all three germ layers including peripheral neurons.

185 into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, m

186 orsal-ventral (DV) gradient across all three germ layers, including the hindgut.

187 the ability to differentiate into all three germ layers, including tissues of endodermal origin (i.e

188 polarized, perpendicular to the plane of the germ layers, independently of Wnt/PCP signaling.

189 neages representative of the three embryonic germ layers indicating the pluripotency of these cells.

190 embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues,

191 been implicated as an important component in germ layer induction and patterning in vertebrate embryo

192 Our results indicate that germ layer induction in the zebrafish tailbud is not a s

193 sence of Wnt signaling, indicating a lack of germ layer induction that normally occurs during gastrul

194 m that naive PSCs do not respond directly to germ layer induction, but must first acquire competence.

195 x regulatory interactions underlying primary germ layer induction.

196 t the early developmental events involved in germ-layer induction in the embryo are recapitulated in

197 ciated with high levels of E-cadherin at the germ layer interface.

198 ormation and ingression, known mechanisms of germ layer internalisation.

199 unction as morphogens to subdivide the three germ layers into distinct territories and act to organiz

200 Formation of the three embryonic germ layers is a fundamental developmental process that

201 otent stem cells differentiate into the main germ layers is a key question of developmental biology.

202 The establishment of the germ layers is amongst the earliest events of developmen

203 of the vertebrate embryo into three primary germ layers is one of the earliest developmental decisio

204 Spatial organization of germ layers is regulated by cortical tension of the colo

205 ells in kidney are derived from an embryonic germ layer known as intermediate mesoderm.

206 emain plastic to transdifferentiation across germ layer lineage boundaries and can be remodeled to ad

207 omic DNA from tissues representing the three germ layer lineages: thyroid (endoderm), heart (mesoderm

208 erm and the endoderm is a location where two germ layers meet and establish an enduring relationship

209 t of the primitive streak and its derivative germ layers, mesoderm and endoderm, are prerequisite ste

210 We traced this phenotype to disrupted germ-layer morphogenesis at the primitive streak.

211 eposited as pillars between widely separated germ layers, namely the somitic mesoderm and the endoder

212 eprogramming in this system, either within a germ layer (NSM transfating) or across a germ layer boun

213 In mammals, specification of the three major germ layers occurs during gastrulation, when cells ingre

214 nts to (or exclude them from) the endodermal germ layer of the zebrafish.

215 ndergo lineage-specific differentiation into germ layers of endoderm, mesoderm and ectoderm during ga

216 cells that can differentiate into all three germ layers of the developing human has fundamentally ch

217 movements during gastrulation establish the germ layers of the vertebrate embryo and coordinate thei

218 s, possibly through indirect effects of this germ layer on neighboring tissues.

219 in treated embryos, but has little effect on germ layer or anterior-posterior markers.

220 fferentiate to form derivatives of the three germ layers organized spatiotemporally, without addition

221 nce that they have a single rather than dual germ layer origin during embryogenesis.

222 Despite the disparate germ layer origins and morphology of the vasculature, th

223 pproaching issues of homology in cases where germ layer origins have shifted during evolution.

224 rowing evidence supports a decoupling of the germ layer origins of the mesenchyme that forms the calv

225 eratomas comprised of tissues from all three germ layer origins suggested that defects in Pofut2 muta

226 es representing differentiation to all three germ layers over the first 3-5 days of LIF withdrawal.

227 Despite their origins in different germ layers, pancreatic islet cells share many common de

228 he Spemann organizer, which is essential for germ layer patterning and axis formation.

229 is an essential signaling center in Xenopus germ layer patterning and axis formation.

230 oogenesis have been reported to function in germ layer patterning, axis determination, and developme

231 f Gtpbp2 causes defects in ventral-posterior germ layer patterning, gastrulation and tadpole morpholo

232 port of RNAs to the vegetal cortex underlies germ layer patterning.

233 nstructions for key aspects of body axis and germ layer patterning; however, the complex genetics of

234 Here, we show that germ-layer patterning in avian gastrulation is ipsilater

235 pmental biology to replicate these organized germ layer patterns in culture.

236 Despite exhibiting germ layer plasticity, these cells never give rise to mi

237 es undergo major rearrangements that lead to germ layer positioning, patterning, and organ morphogene

238 rm a genome-wide transcriptome comparison of germ layer precursor cells.

239 The mesoderm is one of the three germ layers produced during gastrulation from which musc

240 iotemporal pattern of gene expression across germ layers provides evidence that the endoderm was the

241 locus was similar in tissues from the three germ layers, providing evidence that epigenetic patterni

242 movements during gastrulation, cells in both germ layers read their positional information coordinate

243 ecific markers associated with the embryonic germ layers, reminiscent of gastrulating embryos.

244 e specification of cell fate prior to actual germ layer segregation.

245 ate that is responsive to inductive cues for germ layer segregation.

246 agonism during gastrulation may have been in germ-layer segregation and/or epithelial patterning rath

247 However, the cellular and molecular basis of germ-layer segregation is poorly understood, mostly beca

248 tive PCR for the presence of pluripotent and germ layer-specific markers in differentiated ciPSCs; (i

249 In addition, we identified germ-layer-specific H3K27me3 enrichment at sites exhibit

250 ation of gene regulatory networks underlying germ layer specification and axis formation during embry

251 uding symmetry breaking, axial organisation, germ layer specification and cell behaviour, as well as

252 to the post-implantation epiblast, prior to germ layer specification and down-regulation of key plur

253 e positive effects extend beyond the initial germ layer specification and enable efficient differenti

254 d bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis.

255 d study effects on embryonic stem cell (ESC) germ layer specification and mesodermal specification, u

256 hermore, existing models of Dorsal-dependent germ layer specification and patterning consider steady-

257 al role for the let-7 and miR-18 families in germ layer specification and reveal a remarkable conserv

258 In zebrafish, germ layer specification depends on the inheritance of m

259 activate the expression of genes involved in germ layer specification during mESC differentiation in

260 factors are required for axis formation and germ layer specification from sea urchins to mammals.

261 e critical molecular signaling inputs during germ layer specification in bilaterian metazoans, but th

262 contribution of FGF and BMP pathways during germ layer specification in vertebrate embryos.

263 dings present a unique approach to study how germ layer specification is regulated and offer a promis

264 To determine whether germ layer specification is robust to altered cell-to-ce

265 Germ layer specification is tightly coupled with zygotic

266 ET) has a dual role in pluripotency exit and germ layer specification of embryonic stem cells.

267 We discovered that germ layer specification progresses normally in rest mut

268 sensory placode specification, ciliogenesis, germ layer specification).

269 he early embryo to the signals that regulate germ layer specification, and that this early function i

270 l survival, but failed to correct defects in germ layer specification.

271 factors and signaling molecules involved in germ layer specification.

272 netic commitment to differentiation prior to germ layer specification.

273 y in vertebrate embryogenesis by controlling germ layer specification.

274 The Nodal pathway plays a crucial role in germ layer specification.

275 they act as suppressors of commitment during germ layer specification.

276 timate connection between Tfeb/lysosomes and germ layer specification.

277 an development after implantation, including germ-layer specification and axial organization(1-3).

278 qt) and Cyclops (Cyc) - are expressed during germ-layer specification in zebrafish.

279 ing genes in early, uncommitted cells at the germ-layer stage, undergoing profound rearrangements and

280 nt method that employs two custom strains: a Germ Layer strain that expresses nuclear markers in the

281 y structures, and tissues derived from other germ layers such as the pronephros.

282 often, these lineages derive from different germ layers that are specified during gastrulation, well

283 red epithelium is transformed into the three germ layers that are the basis of the embryonic body pla

284 ain approximately 20 cell types across all 3 germ layers, that inter-teratoma cell type heterogeneity

285 Among the three embryonic germ layers, the mesoderm plays a central role in the es

286 criptomics on the topics of the evolution of germ layers, the phylotypic stage, and developmental con

287 onic development is the specification of the germ layers, the subdivision of the blastula embryo into

288 which consist of tissues from two different germ layers; the lateral plate mesoderm-derived mesenchy

289 or beta (TGFbeta) family influence all three germ layers; the ligands are required to induce endoderm

290 hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single

291 oper body architecture and establishes three germ layers through coordinated cellular actions of prol

292 the epiblast generates mesoderm and endoderm germ layers through epithelial-mesenchymal transition (E

293 ct is established and maintained between the germ layers through mesoderm cell protrusions.

294 des evidence that the endoderm was the first germ layer to evolve.

295 hes a novel signaling mechanism that crosses germ layers to diversify bilaterally symmetric neuronal

296 in a short timeframe, the cells of the three germ layers transform into an embryo that includes most

297 iate into derivatives of the three embryonic germ layers when cultured in the appropriate conditions.

298 bodies, expressing genes representing all 3 germ layers when cultured under differentiating conditio

299 ng epithelium is derived from the endodermal germ layer, which undergoes a complex series of endoderm

300 neously differentiate into cell types of all germ layers within embryoid bodies (EBs) in a highly var