ライフサイエンスコーパス: gliadin

1 ally cured, by restoring T-cell tolerance to gliadin.

2 samples at levels comparable with those for gliadin.

3 educed in the presence of partially digested gliadin.

4 -cell factors in driving mucosal response to gliadin.

5 used by ingestion of gluten proteins, mainly gliadin.

6 hat recognized epitopes from gamma-and omega-gliadin.

7 iac disease from the accidental ingestion of gliadin.

8 nd P3 peptides derived from alpha- and gamma-gliadins.

9 surface hydrophobicity of glutenins, but not gliadins.

10 GS, and omega-gliadins over alpha- and gamma-gliadins.

11 8(+) T cell epitopes mapped within the gamma-gliadins.

12 precipitated glutenins more efficiently than gliadins.

13 r time when calculated relative to the alpha-gliadins.

14 A synthetic gene coding for gamma-gliadin 1 (GG1) was expressed in Escherichia coli.

15 Recombinant gamma-gliadin 1 (rGG1) was purified and characterized biochemi

16 e NC-IBS group, 6.51% had antibodies against gliadin, 1.22% against tissue transglutaminase, and 0.61

17 k III as very low molecular weight monomeric gliadins (10-28kDa), peak IV and V, collectively, as alb

18 signified glutenins (30-130kDa), peak II as gliadins (20-55kDa), peak III as very low molecular weig

19 din binds to CXCR3 and that at least 2 alpha-gliadin 20mer synthetic peptides are involved in this bi

20 liac patients by the detection of gluten and gliadin 33-mer equivalent peptidic epitopes (33EPs) in h

21 A model peptide sequenced in alpha-gliadin, 33-mer (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF), was

22 complexes and intestinal permeability to the gliadin 3H-p31-49 peptide were analyzed in polarized mon

23 Our data also suggest that alpha-II-gliadin 62-70 is a DM-suppressed epitope.

24 Levels of IgG directed at gliadin (a component of gluten) and casein (a milk prote

25 ized by intestinal inflammation triggered by gliadin, a component of dietary gluten.

26 an immune-mediated enteropathy triggered by gliadin, a component of the grain protein gluten.

27 Gliadins, a fraction of gluten proteins, are the main co

28 nvestigate the immune reactivity of the anti-gliadin Ab response toward neural proteins.

29 erized the binding of affinity-purified anti-gliadin Abs from immunized animals to brain proteins by

30 Anti-gliadin Abs from patients with celiac disease also bound

31 (13F6 and 14G11) had 35- and 6.6-fold higher gliadin affinities, respectively.

32 Alpha-gliadin affinity column was loaded with intestinal mucos

33 ectrode and the other reports the binding of gliadin after incubation in streptavidin-peroxidase.

34 c disease were immunoglobulins, IgA/IgG anti-gliadin (AGA), and endomysial antibodies (EMA).

35 After gluten gavage, gliadin amount and proteolytic activities were measured

36 leukocyte antigen type and detection of anti-gliadin and anti-transglutaminase 2 antibodies to identi

37 Our results suggest that gliadin and beta-glucan stimulate IL-23 secretion throug

38 T cell lines at levels similar to those for gliadin and caused secretion of cytokines from cultured

39 results suggest that the duplicates of alpha-gliadin and GLR genes have likely taken different evolut

40 bicity, secondary structure, and extractable gliadin and glutenin of gluten were analyzed to elucidat

41 ermus aquaticus, on wheat albumin, globulin, gliadin and glutenin proteins during heat treatment of w

42 Known gliadin and glutenin sequences were largely determined t

43 aricain appears to be capable of detoxifying gliadin and has the potential to mitigate the problems c

44 y using murine monoclonal antibodies against gliadin and high-molecular-weight glutenin subunits.

45 ) or 2-propanol (75 degrees C) decreased the gliadin and increased the glutenin content.

46 We also demonstrated that ID331 omega-gliadin and its derived peptide omega(105-123) exerted a

47 Gliadin and its peptide derivatives, 33-mer and p31-43,

48 Moreover, MC reactivity to gliadin and its peptides was characterized by using in v

49 turn, enables paracellular translocation of gliadin and its subsequent interaction with macrophages

50 d bread wheat in a single wheat line lowered gliadin and low-molecular-weight glutenin accumulation b

51 Marker 1 was identified as omega1,2-gliadin and markers 2, 3a and 3b were identified as gamm

52 ity resulting from molecular mimicry between gliadin and nervous system proteins has been proposed to

53 icain as a means of reducing the toxicity of gliadin and open the way for enzyme therapy as an adjunc

54 emolina gluten proteins consist of monomeric gliadin and polymeric glutenin and determine the quality

55 IA nanoparticles induced unresponsiveness to gliadin and reduced markers of inflammation and enteropa

56 The crucial epitopes that confer toxicity to gliadin and related prolamins continue to be defined, as

57 ted disorder triggered by ingestion of wheat gliadin and related proteins in genetically susceptible

58 tinct cluster containing the wheat protein A-gliadin and the plant viruses potato virus X, narcissus

59 to study the interaction of resveratrol with gliadin and zein.

60 ectants were performed to confirm binding of gliadin and/or 26 overlapping 20mer alpha-gliadin synthe

61 Gliadins and A-gliadin peptide P31-43, in particular, ac

62 everal protein classes, such as LTPs, omega5-gliadins and alpha-amylase/trypsin inhibitors.

63 IgE binding to deamidated gamma- and omega2-gliadins and deamidated total gliadins, frequently with

64 -secalins were most reactive, whereas omega5-gliadins and gamma-, B- and D-hordeins were detected wit

65 Overall, omega1,2-gliadins and gamma-75k-secalins were most reactive, wher

66 branch that existed separate from the gamma-gliadins and gamma-hordeins in an ancestral Triticeae pr

67 ic analysis shows that the orthologous delta-gliadins and gamma3-hordeins form a distinct prolamin br

68 nalyser and Farinograph, respectively, while gliadins and glutenins profiling was done by SDS-PAGE.

69 e interaction of the environmental triggers (gliadins and glutenins) with these gene products to trig

70 c activity and electrophoretic properties of gliadins and glutenins.

71 mainly accumulate in storage proteins called gliadins and glutenins.

72 hile increased protein and all categories of gliadins and high molecular weight glutenins.

73 ponsible for transcriptional derepression of gliadins and low-molecular-weight glutenins (LMWgs) by a

74 tides (a 34-mer and a 26-mer) found in omega-gliadins and low-molecular-weight glutenins that had bee

75 rthered the isolation of the epitopes within gliadin, and other related proteins, that are critical f

76 e addition of DQ8 contributes sensitivity to gliadin, and the addition of the NOD background contribu

77 tissue transglutaminase (tTG) and deamidated gliadin, and the classifier accuracy was independent of

78 the three patients lacking IgE to omega(5) -gliadin, and to HMW glutenin in 59%.

79 nd PFPEQxFP that identified omega- and gamma-gliadins, and their deamidated forms, as immunodominant

80 non-celiac gluten sensitivity patients anti-gliadin antibodies IgG persistence after gluten withdraw

81 Anti-gliadin antibodies of both IgG and IgA classes were assa

82 nts (93.2%) showed the disappearance of anti-gliadin antibodies of IgG class after 6 months of gluten

83 h a high prevalence of first generation anti-gliadin antibodies of IgG class has been reported in thi

84 e the effect of the gluten-free diet on anti-gliadin antibodies of IgG class in patients with non-cel

85 Anti-gliadin antibodies of the IgG class disappear in patient

86 Regarding IgA gliadin antibody determination, sensitivity improved fro

87 Serum samples were analyzed by gliadin antibody enzyme-linked immunosorbent assay, and

88 Measuring total IgA levels, IgG deamidated gliadin antibody tests, and TG2-IgG testing in that circ

89 IgE antibodies to recombinant omega(5) -gliadin are detectable in a majority of WDEIA patients,

90 t human monoclonal antibodies (hmAb) against gliadin are produced by cloning antibody genes from sing

91 The expressed delta-gliadins are encoded by a single gene in each of the hex

92 mins important for its end-use traits, alpha-gliadins are the most abundant, and are also a major cau

93 Gluten proteins, namely gliadins, are the primary trigger of the abnormal immune

94 he immunodominant apolar peptide from alpha2-gliadin as a target for selection.

95 The microarray revealed specifically gamma-gliadin as the second most important allergen.

96 found throughout the immunogenic regions of gliadin, as well as in homologous proteins in barley and

97 inding constant was higher for zein than for gliadin at 35 degrees C.

98 have better diagnostic accuracy over native gliadin-based tests.

99 ynamic parameters suggested that resveratrol-gliadin binding mainly occurs through hydrophobic intera

100 al membrane lysates to identify the putative gliadin-binding moiety.

101 Gliadin binds to CXCR3 and leads to MyD88-dependent zonu

102 n and colocalization experiments showed that gliadin binds to CXCR3 and that at least 2 alpha-gliadin

103 arkers 2, 3a and 3b were identified as gamma-gliadins by means of N-terminal sequence analysis and de

104 adults for whom the specific IgE to omega-5 gliadin can be a useful diagnostic test.

105 We also investigated the effect of gliadin complex formation in intestinal biopsy specimens

106 We measured the effects of gliadin complex formation on cytokine release ex vivo us

107 ansport of secretory immunoglobulin A (SIgA)-gliadin complexes.

108 be focused on a limited region of the alpha gliadin component of gluten, and previous studies have s

109 The number of potential disease-triggering gliadin components remains large.

110 Gliadins contain alpha/beta-, gamma- and omega-types whe

111 susceptible individuals by the ingestion of gliadin-containing grains.

112 w that, under the conditions investigated, A-gliadin contains a considerable amount of hydrated alpha

113 ed assay can be used to quickly estimate the gliadin content in foods in order to protect people with

114 e was found to be more effective in reducing gliadin content than the crude papain and the resultant

115 shown to specifically reduce the immunogenic gliadin content, in gastric conditions, to below the thr

116 ith the amount of toxic epitopes: omega-type gliadins content explain about 40% of the variation of t

117 Extractable gliadins content was affected by the power and time in a

118 obtained after in vitro digestion of a wheat gliadins crude extract and further characterized by LC-E

119 Gliadin-CXCR3 interaction was further analyzed by immuno

120 Triticum aestivum gliadin derived peptides were employed as a positive con

121 e is directed toward an immunodominant alpha-gliadin-derived peptide (DQ8-glia-alpha1).

122 logical effects of ID331 Triticum monococcum gliadin-derived peptides in human Caco-2 intestinal epit

123 For patients with celiac disease, gliadin detoxification via the use of gliadinases may pr

124 Our findings confirmed that ID331 gliadin did not enhance permeability and did not induce

125 positive CD serologic test results (IgA/IgG gliadin, endomysium, or tissue transglutaminase) and com

126 redesign the active site of the acid-active gliadin endopeptidase KumaMax.

127 ll surface, and the presentation of alpha-II-gliadin epitope (residues 62-70) to murine, DQ2-restrict

128 nct from the structures of the TCR:HLA-DQ2.5:gliadin epitope complexes.

129 re we identify microbially derived mimics of gliadin epitopes and a parental bacterial protein that i

130 ted binding to HLA molecules for identifying gliadin epitopes and demonstrate that HLA class I molecu

131 Specific gliadin epitopes are being analyzed.

132 innate and adaptive immune responses to key gliadin epitopes are now both recognized to be important

133 The gliadin epitopes were found to have moderate affinity fo

134 eraction in the generation of DQ2-restricted gliadin epitopes, relevant to celiac disease, or DQ2-res

135 imicry underpins cross-reactivity toward the gliadin epitopes.

136 g the in situ T-cell recognition of dominant gliadin epitopes.

137 fter intracellular generation of four tested gliadin epitopes.

138 ens tissue transglutaminase-2 and deamidated gliadin, exhibiting 71% sensitivity and 99% specificity

139 city of 92.9%, which was higher than that of gliadin extract (e).

140 he best characterized environmental factors (gliadin) for any common autoimmune disease.

141 capable of degrading >99% of the immunogenic gliadin fraction in laboratory-simulated gastric digesti

142 Wheat, specifically its omega-5 gliadin fraction, is the most common allergen implicated

143 showed that the chromatograms of the reduced gliadin fractions were most suitable for the distinction

144 A gliadin fragment can activate the innate immune system,

145 und to directly respond to nonimmunodominant gliadin fragments by releasing proinflammatory mediators

146 ma- and omega2-gliadins and deamidated total gliadins, frequently with high concentrations.

147 Samples of hydrolyzed gliadin from wheat and a barley beer were used.

148 vation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines.

149 amples before and after microwave treatment, gliadins from treated flours showed significantly reduce

150 immunodetection with specific antibodies for gliadins, gamma-gliadins, LMW subunits and antigenic epi

151 This single delta-gliadin/gamma3-hordein ortholog may be a general feature

152 b region from Ae. tauschii locates the delta-gliadin gene to the complex Gli-1 plus Glu-3 region on c

153 r the detection of T. aestivum, based on the gliadin gene, is inadequate.

154 which have given rise to 12 copies of alpha-gliadin genes clustered within a 550-kb region.

155 cies estimated that between 25 and 150 alpha-gliadin genes reside in the Gli-2 locus regions.

156 sequences also allow assignment of the delta-gliadin genes to the A and D genomes, respectively, with

157 tionary relationship of the duplicated alpha-gliadin genes were obtained from their genomic organizat

158 copies that are interspersed with the alpha-gliadin genes.

159 in particular tissue transglutaminase (tTG), gliadin (Glia), and endomysium.

160 To clarify the conformational changes of gliadins (Glia) upon complexation with anthocyanidins (i

161 3.8 mumol N-ethylmaleimide/g protein reduces gliadin-glutenin cross-linking during pasta drying and/o

162 g 13.8 mumol glutathione/g protein increases gliadin-glutenin cross-linking during pasta processing,

163 45( * *)), gluten index (r=0.959( * *)), and gliadin/glutenin ratio (r=-0.952( * *)), while peak II i

164 r composition in terms of protein fractions (gliadins, glutenins) has been determined by means of RP-

165 microarray immunoassay with alpha/beta/gamma-gliadin, high-molecular-weight (HMW) glutenin, alpha-amy

166 Levels of anti-gliadin IgG (but not anti-casein IgG) above the 90th per

167 High levels of anti-gliadin IgG in the maternal circulation are associated w

168 were not related to maternal levels of anti-gliadin IgG.

169 l IgA, or IgA-TTG and IgG against deamidated gliadin (IgG-DGL) could identify patients with and witho

170 tions of CD4(+)CD62L(-)CD44(hi) T cells from gliadin-immunized C57BL/6 mice and were fed with an AIN-

171 d paracellular permeability, normalized anti-gliadin immunoglobulin A in intestinal washes, and modul

172 in 14/17 patients (82%), to alpha/beta/gamma-gliadin in 82% including the three patients lacking IgE

173 truncated an aptamer capable of recognizing gliadin in a deep eutectic solvent (DES).

174 me mice were given intradermal injections of gliadin in complete Freund's adjuvant (immunization) or

175 This sensor can detect gliadin in DES, with a dynamic range between 1 and 100 m

176 al diagnostic value of alpha/beta- and gamma-gliadin in particular in omega(5) -gliadin-negative pati

177 ration of proteases that can rapidly degrade gliadin in the gastric compartment has been proposed as

178 wing model for the innate immune response to gliadin in the initiation of CD.

179 oss-linking of glutenins and of inclusion of gliadin in the polymers was seen for SB6 as compared to

180 yme caricain (from papaya latex) to detoxify gliadin in whole wheat flour and develop bread suitable

181 s able to detoxify a major proportion of the gliadin in wholemeal wheat dough by allowing it to react

182 The absence of extractable alpha- and gamma-gliadins in DDGS indicated protein aggregation during th

183 utaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated forms) before T-cell c

184 We hypothesize that too much gliadin incorporation in the glutenin network during pas

185 ations were explained as resulting from less gliadin incorporation in the polymer gluten network and

186 Furthermore, binding between resveratrol and gliadin increased at higher temperatures, which was not

187 Gliadin increased zonulin release and intestinal permeab

188 Gliadin induced physical association between CXCR3 and M

189 ride A improved barrier function and reduced gliadin-induced cytokine secretion.

190 Gliadin-induced zonulin release, increased intestinal pe

191 Gliadin induces an MyD88-dependent zonulin release that

192 We demonstrate that wheat gliadin induces significantly greater production of IL-2

193 study, we sought to test the hypothesis that gliadin initiates this response by stimulating the innat

194 The problem of gliadin insolubility was solved by mild acid treatment,

195 We aimed to establish the molecular basis of gliadin interaction with intestinal mucosa leading to in

196 Gliadin interaction with the intestinal epithelium incre

197 ogen bonding, whereas their interaction with gliadins is dominated by hydrogen bonding and is relativ

198 d wheat prolamin class, given the name delta-gliadins, is the most direct ortholog of barley gamma3-h

199 with specific antibodies for gliadins, gamma-gliadins, LMW subunits and antigenic epitopes to gain a

200 DQ2alpha+53G mutant further suppressed these gliadin-mediated responses.

201 ), the HLA-DQ8 transgenic (model 2), and the gliadin memory T-cell enteropathy (model 3) models of ce

202 and gamma-gliadin in particular in omega(5) -gliadin-negative patients in the diagnosis of WDEIA.

203 ed for IgA and IgG antibodies against native gliadin (ngli) and deamidated gliadin peptides (dpgli),

204 , mitigating the injury of Triticum aestivum gliadin on cell viability and cytoskeleton reorganizatio

205 The effects of gliadin on crypt enterocyte proliferation and activation

206 ents were set up to assess the effects of PT gliadin on innate and adaptive immune response by using

207 rees C (65% relative humidity (RH)), whereas gliadin only is incorporated in the protein network at t

208 ere calculated for levels of IgG directed at gliadin or casein for nonaffective psychosis.

209 eund's adjuvant (immunization) or of soluble gliadin or ovalbumin (ear challenge).

210 videnced by strong IgE binding to deamidated gliadins or peptides of the type QPEEPFPE.

211 subunits (HMW-GS) over low MW-GS, and omega-gliadins over alpha- and gamma-gliadins.

212 ater than placebo (wheat, P = .0005; omega-5 gliadin, P = .0001).

213 (130-30 kDa; glutenins), peak II (55-20 kDa; gliadins), peak III (28-10 kDa; low molecular weight gli

214 ), peak III (28-10 kDa; low molecular weight gliadins), peak IV and V (<10 kDa; albumins and globulin

215 st tissue transglutaminase (TG2), deamidated gliadin peptide (DGP) and endomysium (EMA).

216 intestinal permeability of undegraded alpha9-gliadin peptide 31-49 (p31-49) and 33-mer gliadin peptid

217 tudy participants, mean levels of deamidated gliadin peptide autoantibodies were 7.46 (6.92) in patie

218 odies, in disease pathogenesis, by enhancing gliadin peptide class II binding and consequent T-cell a

219 Gliadins and A-gliadin peptide P31-43, in particular, act as growth fac

220 proliferation induced by EGF, IL15, and the gliadin peptide P31-43.

221 nst tissue transglutaminase-2 and deamidated gliadin peptide), symptom frequencies, and safety.

222 sue transglutaminase, IgA against deamidated gliadin peptide, and endomysial antibody (IgA).

223 ainst tissue transglutaminase and deamidated gliadin peptide, greatly facilitate diagnosis.

224 gainst tissue transglutaminase or deamidated gliadin peptide, or endomysial antibody, should be consi

225 treatment, which renders an acid-hydrolysed gliadin/peptide mixture (AHG).

226 f CeD derived from neoepitopes of deamidated gliadin peptides (DGP) and tTG fragments and to determin

227 The detection of IgG against deamidated gliadin peptides (DGP) has high specificity and better s

228 detection of serum antibodies to deamidated gliadin peptides (DGPs).

229 against native gliadin (ngli) and deamidated gliadin peptides (dpgli), as well as for IgA antibodies

230 182 Da; horseradish peroxidase, 40 kDa] and gliadin peptides [33-mer (p56-88, 3900 Da), 19-mer (p31-

231 The mechanism of translocation of gliadin peptides across the intestinal barrier has been

232 Novel deamidated gliadin peptides antibodies have better diagnostic accur

233 progress has been made in understanding how gliadin peptides can cross the intestinal border and acc

234 residues of the TG2 epitope with deamidated gliadin peptides could be a structural basis.

235 clones specific for dominant alpha- or omega-gliadin peptides from children with celiac disease had c

236 Glutamine-rich gliadin peptides from ingested cereals, after their deam

237 proliferation and innate immune response to gliadin peptides in enterocytes.

238 cause of intestinal cell stress reports that gliadin peptides inhibit the function of the chloride an

239 a9-gliadin peptide 31-49 (p31-49) and 33-mer gliadin peptides is increased in active celiac disease.

240 ases in transcellular permeability to intact gliadin peptides might be considered in treatment strate

241 ation of infiltrating MCs and the effects of gliadin peptides on intestinal MCs indicated an increase

242 One recent study using diamidated gliadin peptides showed good specificity (> or = 0.94),

243 uten epitopes, degradation of immunodominant gliadin peptides that resist intestinal proteases by exo

244 These 23 gliadin peptides were next assayed by IFN-gamma ELISPOT

245 inding to apical CD71, SIgA (with or without gliadin peptides) enters a recycling pathway and avoids

246 1 for TGA-IgG, 6 for IgG against deamidated gliadin peptides, and 1 for EMA, from 5 different manufa

247 nd CD71 promote transepithelial transport of gliadin peptides.

248 not be considered markers of permeability to gliadin peptides.

249 ve and innate immune responses to undigested gliadin peptides.

250 gen, tissue transglutaminase, and deaminated gliadin peptides; the most frequently detected nonmitoch

251 Undigested gliadin proline-rich peptides trigger the innate and ada

252 de-co-glycolide) nanoparticles encapsulating gliadin protein (TIMP-GLIA) in 3 mouse models of celiac

253 We screened gliadin proteins (2alpha-, 2omega-, and 2gamma-gliadin)

254 pitope was found on native gamma- and omega2-gliadins (QPQQPFPQ); it was repeated several times in th

255 ed by antigen-presenting cells and activated gliadin reactive HLA-DQ2.5-restricted T cells derived fr

256 Accordingly, gliadin reactive T cells involved in CeD pathogenesis cr

257 assay test kit was used for the analysis of gliadin residues in the baked bread.

258 llular route, this appears to be enhanced by gliadin's stimulation of zonulin release.

259 In mice with gliadin sensitivity, injection of TIMP-GLIA nanoparticle

260 A fourth delta-gliadin sequence occurs in the D genome of both Chinese

261 Epitopes were mapped on gamma- and omega2-gliadin sequences by Pepscan, and effect of glutamine/gl

262 olayers derived from CD organoids exposed to gliadin showed increased intestinal permeability and enh

263 The patterns of the reduced spelt gliadins showed one to three markers that were not prese

264 specific (rho = 0.55, P = .0003) and omega-5 gliadin-specific (rho = 0.51, P = .001) IgG(4) levels in

265 of intraepithelial lymphocyte cytotoxicity, gliadin-specific antibodies, and a proinflammatory gliad

266 from their serum to inhibit the binding of a gliadin-specific hmAb (1002-1E03) to a specific peptide

267 prick test responses and wheat- and omega-5 gliadin-specific IgE levels did not differ between group

268 cretion (in models 1, 2, and 3), circulating gliadin-specific IgG/IgG2c (in models 1 and 2), ear swel

269 oduction and the proliferation of intestinal gliadin-specific T cell lines and clones were measured a

270 The alpha-gliadin-specific T cells from children had biases in T-c

271 ections of TIMP-GLIA significantly decreased gliadin-specific T-cell proliferation (in models 1 and 2

272 n-specific antibodies, and a proinflammatory gliadin-specific T-cell response.

273 lows the measurement of as low as 0.5 ppb of gliadin standard (0.5 ppm of gluten).

274 The use of a gliadin standard in R5 ELISA determinations causes an ov

275 The gliadins structure appeared to be progressively disorgan

276 of gliadin and/or 26 overlapping 20mer alpha-gliadin synthetic peptides to the receptor.

277 ater binding affinity for both glutenins and gliadins than lower MW PA, whereas both PA precipitated

278 eat encoding the most immunogenic peptide of gliadin that triggers the immune response of celiac dise

279 and styrene sulfonate complex with isolated gliadin (the toxic fraction of gluten) and prevent damag

280 On ingestion of gliadin, the major protein component of wheat and other

281 iadin with Kuma030 eliminates the ability of gliadin to stimulate a T cell response.

282 sing two cut-off values (10 and 50 mg kg(-1) gliadin) to provide a semi-quantification for identifyin

283 rgets of the hmAb 1002-1E03 from a digest of gliadin treated by transglutaminase 2.

284 We carried out quantitative analysis of gliadin types by RP-HPLC to verify its correlation with

285 gens Ara h 1 and Ara h 2, and wheat allergen Gliadin using giant magnetoresistive (GMR) sensor arrays

286 iadin proteins (2alpha-, 2omega-, and 2gamma-gliadin) using bioinformatic algorithms for the presence

287 The specific IgE to omega-5 gliadin was below the detection limit in all cases.

288 Actinidin efficacy in hydrolysing gliadin was compared with that of other gluten-degrading

289 By ImmunoCAP, IgE to recombinant omega(5) -gliadin was detectable in 14/17 patients (82%), to alpha

290 e 96-well-based ELISA developed in-house for Gliadin was found to have a LOD of 40 ng/mL.

291 The rate of gastric degree of hydrolysis of gliadin was greater (P < 0.05) by actinidin (0.8%/min) w

292 As a result of its use, a lower level of gliadin was incorporated into the gluten polymer and dou

293 0 the original molecular weight while native gliadin was nearly eliminated from the waxy flour follow

294 thermal dependent behavior of glutenins and gliadins was analyzed through intrinsic fluorescence par

295 f DME silencing on accumulation of LMWgs and gliadins was studied using 20 transformants expressing h

296 d assays developed for Ara h 1, Ara h 2, and Gliadin were 7.0 ng/mL, 0.2 ng/mL, and 1.5 ng/mL, respec

297 epitopes of alpha-/beta-, gamma- and omega5-gliadins were highlighted after performing molecular dyn

298 As glutenin-bound omega-gliadins were present in wheat and absent in spelt, this

299 A culture medium, in which gliadins were the sole source of nitrogen, was developed

300 Indeed, treatment of gliadin with Kuma030 eliminates the ability of gliadin t

301 There, the interaction of gliadin with macrophages elicits a MyD88-dependent proin