ライフサイエンスコーパス: glycan

1 l Gal residue to construct a heptasaccharide glycan.

2 ilar to that of the recombinant glycoprotein glycans.

3 cosyl hydrolase that cleaves peptide-free PG glycans.

4 cells, which was dependent on viral N-linked glycans.

5 glycan conformations and one system without glycans.

6 samples for separation of glycopeptides and glycans.

7 s building of ordered acidic side chains and glycans.

8 onal modification, notably asparagine-linked glycans.

9 site of neuraminidase due to the presence of glycans.

10 sialic acid, galactose, and GalNAc levels in glycans.

11 e virus from being captured by the synthetic glycans.

12 thesis of other structurally related complex glycans.

13 approach for the characterization of unknown glycans.

14 +) and in complex with both UDP:Mg(2+) and a glycan acceptor, lacto-N-neotetraose.

15 y explains the specificity for both N- and O-glycan acceptors.

16 In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the h

17 n N2, suggesting N2 is more accommodating to glycan additions.

18 It also revealed that the head domain glycans affect N1 stability more than N2, suggesting N2

19 ntifiable, site-specific, disease-associated glycan alterations for clinical applications, we have ad

20 N-glycan alterations in the nervous system can result in d

21 Differentially expressed N-glycans among the tumor region, adjacent normal tissue r

22 Our approach to glycan analysis combines ultrahigh-resolution IMS-IMS us

23 R) spectroscopy is a promising technique for glycan analysis, as it provides unique vibrational finge

24 ge, demonstrating the power of this tool for glycan analysis.

25 se treatment, we have now investigated TfR N-glycan and GPI processing.

26 ated cell membrane glycoproteins following N-glycan and lipid/glycolipid removal by PNGase F digestio

27 AOGP utilized de novo sequencing for O-glycans and a database search strategy for peptide backb

28 e tumor cells thus express normal extended O-glycans and become more sensitive to TRAIL treatment.

29 trains do not have the required high-mannose glycans and do not interact with SP-D, and that sequence

30 ection limit of 100 attomole is attained for glycans and furthermore 58 glycans are enriched from hum

31 graphitized carbon (PGC) column, while the N-glycans and glycolipids isolated from the same cell memb

32 be how IMS is able to distinguish isomeric N-glycans and glycopeptides using both intact IMS and frag

33 ique should be applicable to other cells and glycans and provides a way to expand the repertoire of g

34 s layer allows bacteria to degrade the mucin glycans and recover the energy content that is then shar

35 Profiling of the glycans and the glycolipids is performed using nanoflow

36 of both tumor-associated truncated O-linked glycans and their receptor, macrophage galactose-type le

37 s biology are facilitating novel insights on glycans and their relationship with multicellular behavi

38 varying in three distinct features: aglycon, glycan, and amine substitution pattern.

39 hat rely on the integrated roles of ATP6AP1, glycans, and lipids.

40 expressed proteins used in NMR studies lack glycans, and proteins from other organisms are neither (

41 glycans results in significant increases of glycan anionic signals as compared to that using the tra

42 Interestingly, some glycans appear modified with a group of 144 Da, whose id

43 e is attained for glycans and furthermore 58 glycans are enriched from human serum.

44 Mammalian Asn-linked glycans are extensively processed as they transit the se

45 Sialylation and sialic acid linkage in N-glycans are markers of disease but are analytically chal

46 Glycans are present on neuraminidase and are generally c

47 Functionally important AGP glycans are synthesized in the Golgi apparatus, but the

48 Using glycan arrays, we dissected IgE responses to specific gl

49 ell cultures to generate usable amounts of O-glycans as a potential O-glycome factory.

50 hrough recognition of sialic acid-containing glycans as ligands, they help the immune system distingu

51 Low abundant glycans as well as additional post-translational and che

52 ite-specific inter-protein and intra-protein glycan associations, of which the vast majority were pre

53 ich are modified with a branched, heptameric glycan at all N-linked sequons except for the site close

54 Here, we show that introducing the group 2 glycan at Asn38(HA1) to a group 1 stem-nanoparticle (gN3

55 ibodies that recognized all of the synthetic glycans at 1:200-fold dilution.

56 microscope to investigate interactions with glycans at the single-virion level directly on living ma

57 rface, implying the presence of reducing-end glycans at this location where the capsule is attached t

58 Glycans attached to lipids and membrane-bound and secret

59 Also, N-glycans attached to misfolded AAT are not required for a

60 The glycans attached to proteins represent an important dete

61 ntal and applied clinical studies, including glycan-based biomarker discovery and therapeutics.

62 This mechanism of glycan-based receptor usage, entailing a concerted, fine

63 The synthetic DSGb5 glycan bearing a 6-azidohexyl aglycon at the reducing en

64 ificity of the toxin are related to specific glycan binding characteristics of the toxin.

65 discovery of two previously unknown surface glycan binding proteins which facilitate glycosaminoglyc

66 dicates that this region likely contains the glycan binding site.

67 d identify structural determinants promoting glycan binding to glycan-binding proteins due to the amb

68 rrent GlyMDB provides data visualization and glycan-binding motif discovery for 5203 glycan microarra

69 r/non-binder threshold followed by a list of glycan-binding motifs.

70 ltB subunit of each toxin exhibits different glycan-binding preferences that correlate with glycan ex

71 LGALS2 encodes the glycan-binding protein Galectin 2 (Gal2), which is predo

72 Galectin-1 (Gal1), an endogenous glycan-binding protein, has emerged as a regulator of im

73 ological processes through interactions with glycan-binding proteins (GBPs).

74 ral determinants promoting glycan binding to glycan-binding proteins due to the ambiguity in microarr

75 d provides a way to expand the repertoire of glycan-binding proteins for further study.

76 ctins, an evolutionarily conserved family of glycan-binding proteins, have broad influence in tumor p

77 une responses by binding to immunoregulatory glycan-binding receptors on immune cells.

78 h AAT and is only partially dependent on the glycan-binding site of CRT, which is generally relevant

79 anStructure.ORG for further investigation of glycan-binding sites and glycan structures.

80 nitoring mass spectrometry method for use in glycan biomarker research.

81 Providing further challenges, glycan biosynthesis and cellular behavior are co-regulat

82 Blocking N-glycan biosynthesis at the oligomannose stage using both

83 Among them, inhibition of N-glycan biosynthesis enhanced Spike-protein proteolysis.

84 ewly available datasets and tools, including glycan biosynthesis models, omics datasets, and systems-

85 t the V3-glycan patch that resemble human V3-glycan bNAbs.

86 To identify the types of glycans bound by CHIKV, we conducted glycan microarray a

87 However, N-glycan branching was not impaired in these cells.

88 GlcNAc is a rate-limiting metabolite for N-glycan branching.

89 the influenza A virus (IAV), large N-linked glycans can also be added to prevent access to epitopes

90 Finally, the GNP-glycans can potently and completely inhibit DC-SIGN-medi

91 cathepsins in acidic endosomes, removing the glycan cap and exposing a binding site for the Niemann-P

92 ecific mAbs, one base binding (16F6) and one glycan cap binding (X10H2), was down-selected for assess

93 Thus, the glycan cap of GP1 may allosterically protect against ina

94 result supported the idea that elongation of glycan chains has to proceed from the reducing to the no

95 esults in the formation of oligomannose-type glycan clusters, which were absent on SARS and HKU1 CoVs

96 sialylation showed selectivity toward the N-glycan compared to another glycan substrate.

97 ues, define atomic-level structures of virus-glycan complexes, and study these interactions at the si

98 We found that the N-glycan composition of Lu. longipalpis saliva mostly cons

99 ing effector cells is sufficient to affect N-glycan composition.

100 usion proteins influence not only individual glycan compositions but also the immunological pressure

101 es consisting of four systems with different glycan conformations and one system without glycans.

102 f the technique enables the visualization of glycan connectivity and discrimination between regioisom

103 cell surface, whether and if so how these N-glycans contribute to monocyte recruitment is not known.

104 4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.

105 nd that the observed Golgi localization of O-glycan-deficient cargos is due to their slow Golgi expor

106 n against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.

107 We provide a comparison of the global glycan density of coronavirus spikes with other viral pr

108 Here, we reveal a specific area of high glycan density on MERS S that results in the formation o

109 Ab1485 binds the V3-glycan epitope in a glycan-dependent manner.

110 ing and highly reactive fluorescent tags for glycan derivatization.

111 binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.

112 tes hampers the characterization of specific glycan determinants.

113 However, whether IgE glycans differ in disease states or affect biological ac

114 ectroscopy of the protein indicates that the glycans do not influence its fold.

115 Blocking O-glycan elaboration also partially blocked viral entry.

116 We describe a monomeric, glycan-engineered RBD protein fragment that is expressed

117 th targeted glycan deletion and high-mannose glycan enrichment.

118 We identify the glycan epitope high mannose as a marker of influenza vir

119 Ab1485 binds the V3-glycan epitope in a glycan-dependent manner.

120 olvent as well as the enzymatic synthesis of glycan epitopes in the aqueous phase in a single reactio

121 and differential increases in complex hybrid glycans, especially for KRAS and HER2 oncogenes.

122 ycan-binding preferences that correlate with glycan expression profiles of host cells targeted by eac

123 The alteration of sialylated N-glycan expressions is important to detect the immune sta

124 II but fixed (unable to phase-vary) LOS HepI glycans extended beyond the lactose substitution of HepI

125 hylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrat

126 r GalNAc-LNnT), were used to define how HepI glycan extensions affect (i) mouse vaginal colonization

127 Glycan extensions beyond lactose on HepI modulate bindin

128 The glycan family abundance on individual proteins showed su

129 on biosynthesis, physiological context, and glycan fine structure.

130 glioblastomas exploit cell surface O-linked glycans for local and distant immune modulation.

131 m a filamentous fungus and used in crystallo glycan fragment screening to reassemble the GPI-core gly

132 identical to a structure of hormone-bound, N-glycan-free ECD, which suggested that the GlcNAc might a

133 here we conducted an in-depth analysis of N-glycans from a brittle star (Ophiactis savignyi) as an e

134 (MALDI-MSI) was developed for analysis of N-glycans from FFPE treated tissue sections.

135 Piperazine tips also enrich glycans from ovalbumin and human immunoglobulin G.

136 strains, the P[8]-4 VP8* protein attached to glycans from saliva samples regardless of the donor's se

137 Compositions of the N-linked glycans from the native spikes were analyzed by mass spe

138 Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia lon

139 lysis of the N- and O- and glycosphingolipid-glycans from total human lungs, along with histological

140 cluding initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host

141 thod enables a high-throughput enrichment of glycan, glycosites, and IGPs from biological samples.

142 a high-throughput method for characterizing glycans, glycosites, and intact glycopeptides (IGPs) der

143 We observed that N- and O-glycans had only minor contribution to Spike-ACE2 bindin

144 e, two on each heavy chain, of which the Fab glycans have been reported to be complex and multiply si

145 aled overall processing states of the native glycans highly similar to that of the recombinant glycop

146 nstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences

147 olution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactiv

148 the molecular basis of this strain-specific glycan hole response.

149 We conclude that even for the immunodominant glycan hole shared between BG505 and B41, the prospect o

150 l antibody and was able to achieve sensitive glycan identification at a low microgram level of glycop

151 n a prerequisite to ensure the comprehensive glycan identification.

152 ve CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.

153 ragment screening to reassemble the GPI-core glycan in a U-shaped conformation within its binding poc

154 Elimination of branched N-glycans in developing B cells via targeted deletion of N

155 of the three-dimensional (3D) arrangement of glycans in dictating GBP interactions, strategies that a

156 ural modeling analyses directly implicated N-glycans in Klotho's protein folding and function.

157 However, the role of glycans in membrane trafficking is still unclear.

158 ze approaches for disentangling the roles of glycans in multicellular interactions using newly availa

159 dies have reported the characterization of N-glycans in rodent brains, but there is a lack of spatial

160 ycans with the detection of phosphorylated N-glycans in SN which were not detected in the striatum.

161 to bead saturation or hindrance by existing glycans in the matrix that precluded the virus from bein

162 ycan structures, the limited availability of glycans in their purified form, the low affinities of GB

163 g Tn and STn antigens to study the role of O-glycans in TRAIL-induced apoptosis.

164 controls synapse development through protein-glycan interaction and identify it as a potential therap

165 new perspectives to better understand virus-glycan interactions in physiologically relevant conditio

166 icosahedral MCPyV capsid and analysis of its glycan interactions via nuclear magnetic resonance (NMR)

167 eir purified form, the low affinities of GBP-glycan interactions, and limitations in existing binding

168 Here, the glycan interacts with the receptor-binding domain D of t

169 M. thermolithotrophicus archaellin N-linked glycan is larger and more complex than those previously

170 (polySia), a homopolymer of alpha2,8-linked glycans, is a posttranslational modification on a few gl

171 thesize that region-specific resolution of N-glycans isolated from the striatum and substantia nigra

172 unique vibrational fingerprints of specific glycan isomer ions.

173 High-resolution separation of glycan isomers differentiating from positional, linkage,

174 his method, we identified 44 cell membrane O-glycan isomers with MS/MS, and, among them, we unambiguo

175 e enzymes that specifically cleave the algal glycan laminarin into readily analyzable fragments.

176 They can not only quantify the GNP-glycan-lectin binding affinities via a new fluorescence

177 The glycan ligand information is displayed, and links are pr

178 scaling the relative abundances of released glycan ligands according to their relative abundances in

179 However, uncovering functional glycan ligands is challenging due to the large number of

180 d library, allows the relative affinities of glycan ligands to be ranked.

181 1-glycosylation sites and the Asn323-/Asn483-glycans, located in the MPO dimerisation zone, was found

182 The clustered and variable O-glycans make the IgA1 glycomic analysis challenging and

183 l((13)C(6))-Tn, which gives rise to a unique glycan mass.

184 The laminin-binding glycan (matriglycan) on alpha-dystroglycan (alpha-DG) en

185 Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, sh

186 ypes of glycans bound by CHIKV, we conducted glycan microarray analyses and discovered that CHIKV pre

187 and glycan-binding motif discovery for 5203 glycan microarray samples collected from the Consortium

188 pared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities o

189 ions in antibodies, as defined by binding to glycan microarrays and by affinity purification.

190 simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of th

191 ith these data, we investigated the level of glycan microheterogeneity within the urinary exosomes, f

192 s This could indicate that the nature of the glycan modification may have a role to play in maintaini

193 Glycans modify proteins required for regulation of immun

194 rays, we dissected IgE responses to specific glycan moieties and found that reactivity to classical C

195 e basic components of the glycocalyx and the glycan moieties implicated in cancer.

196 to program the most informative MS(3) on the glycan moiety itself.

197 tions allow for highly precise site-specific glycan monitoring with minimum sample prep.

198 gether, these data demonstrate that discrete glycan motifs expressed on CD11b/CD18 such as biantennar

199 e resources enable one to identify and study glycan motifs involved in immunogenicity, pathogenicity,

200 ation and the synthesis of complex antennary glycan motifs.

201 e presence or absence of galactose on the Fc glycan of IgG1 did not alter FcgammaRIIIa or FcRn bindin

202 ctivities involved in the synthesis of the N-glycan of the viral major capsid protein in PBCV-1 and e

203 owed glucose as the major component in the O-glycans of the three SLPs; however, some differences in

204 that are carried on both the N- and O-linked glycans of VWF.

205 ecifically synthesize a larger repertoire of glycan oligomers by partitioning promiscuous enzymes acr

206 it the secretory pathway to generate diverse glycans on cell surface and secreted glycoproteins.

207 The glycans on lubricin have also been suggested to be invol

208 In this work, we studied the effect of glycans on the binding kinetics of antiviral drugs to th

209 y of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.

210 can types as well as the specific individual glycans on the modification sites of the ectodomain and

211 The presence of host glycans on the proteins of microbial origin may prevent

212 at macaques can develop bNAbs against the V3-glycan patch that resemble human V3-glycan bNAbs.

213 Cetuximab harbors four glycans per molecule, two on each heavy chain, of which

214 the urinary exosomes, finding on average 5.9 glycans per site.

215 Mucin-type O-glycans play key roles in many cellular processes, and t

216 erized, the steps involved in terminating PG glycan polymerization remain poorly understood.

217 se treatment, which digests anionic sulfated glycan polymers, before exposure rendered cells insensit

218 We demonstrate that O-glycans positioned within the receptor binding motifs of

219 ensity gradient centrifugation, and N- and O-glycans present in each compartment were analyzed by LC-

220 two-step enzymatic procedure to transform Fc-glycans present on IgG mAbs into two site-specific ancho

221 BP receptors in live cells, where the native glycan presentation and glycoprotein expression are pres

222 nt increases in E6 size consistent with post-glycan processing in the endoplasmic reticulum.

223 Transcripts associated with N-glycan processing were downregulated while those associa

224 risation zone, was found to affect the local glycan processing, thereby providing a molecular basis o

225 We further explored its application in the N-glycan profile of a biotherapeutic monoclonal antibody a

226 No differences in IgG1 Fc glycan profiles and minimal differences in IgG2 Fc glyca

227 M method for examination of residue-specific glycan profiles of EPO was established.

228 ) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structu

229 labels, we demonstrate click-ExM on lipids, glycans, proteins, DNA, RNA and small molecules.

230 ach, we report novel functional roles of MPO glycans, providing new insight into neutrophil-mediated

231 is now possible to rapidly identify specific glycan receptors using different techniques, define atom

232 onic effects in dictating the specificity of glycan recognition by proteins.

233 Correction for 'The challenges of glycan recognition with natural and artificial receptors

234 tion/Michael addition, are not specific to O-glycan release and can also eliminate phosphoryl substit

235 dy responses to the site of vulnerability by glycan repositioning may be a step towards achieving cro

236 he negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acid

237 APOE structures with various glycan residues were predicted.

238 Coupling 2-AA to glycans results in significant increases of glycan anion

239 er than electron-driven dissociation because glycan-retaining peptide fragments would not be required

240 ion sites to ST6GAL1, we demonstrated that O-glycan's effect on Golgi export is probably additive.

241 However, the glycan's structure has not been described so far.

242 on of N- and O-linked glycopeptides and open glycan searches.

243 using both intact IMS and fragment-based IMS glycan sequencing experiments in positive ion mode, with

244 idered to inhibit antibody binding via their glycan shield.

245 izer, AMC011, in having a dense and complete glycan shield.

246 fferences in complex and oligomannose type N-glycans, sialylation (mono-, di-, and tetra-), fucosylat

247 Specific glycan sites and amino acids located at the tip of the H

248 hey naturally produce high quantities of the glycan-specific antibodies that can be protective agains

249 ctic activity of IgE may be downregulated by glycan-specific IgG anti-IgE autoantibodies.

250 The MHC-I-linked glycan steers a tapasin loop involved in peptide editing

251 ty by analyzing glycoproteomes with uncommon glycans stemming from the green alga Chlamydomonas reinh

252 pite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that contro

253 ilize two types of synthases that polymerize glycan strands and crosslink them: class A penicillin-bi

254 se proteins coordinate polymerization of new glycan strands with their crosslinking to the existing p

255 metry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to

256 Here, we quantified a glycan structural type using a recently developed biocat

257 lotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose d

258 ent the first mechanistic insight into how O-glycan structures on cell surface modulate their sensiti

259 We first quantify the Core 1 and 2 O-glycan structures on the carbohydrate recognition and ex

260 f O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.

261 distinguished by abundant and unique surface glycan structures that are rich in xylose.

262 Following injection, only afucosylated N-glycan structures were passed through enzyme zones that

263 ng them, we unambiguously characterized 25 O-glycan structures with exoglycosidase digestion to creat

264 e to the large number of naturally occurring glycan structures, the limited availability of glycans i

265 escence intensity and complexity in branched glycan structures.

266 er investigation of glycan-binding sites and glycan structures.

267 vity toward the N-glycan compared to another glycan substrate.

268 Our results also demonstrate that glycan subtype can be predicted at some glycosites based

269 SP-D, and that sequence analysis can predict glycan subtype, thus predicting the presence or absence

270 that would ideally be specific for distinct glycan subtypes.

271 protein is modified with the same heptameric glycan, suggesting a common N-glycosylation pathway.

272 nsferase that initiates complex and hybrid N-glycan synthesis.

273 of PSA interactions and achieving selective glycan targeting.

274 r cases they suggested the presence of novel glycan targets on many of the microorganisms.

275 distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass

276 ches also revealed many sulfated and complex glycans that remained hidden to the original search.

277 number of high mannose, hybrid and complex N-glycans that were localized to regions of mucus and alve

278 mass spectrometry imaging to spatially map N-glycans to distinct pathological alterations during the

279 Here, we add two artificial glycans to uniformly (15)N labeled prion protein using a

280 specific types of GAGs and potentially other glycans to which CHIKV binds and whether there are strai

281 ndo-acting glycoside hydrolase involved in N-glycan trimming and is located within the Golgi, where i

282 iations were observed in the distribution of glycan types as well as the specific individual glycans

283 The selectivity for different glycan types was studied using bovine fetuin, asialofetu

284 rtantly, direct elimination of one or more O-glycans under negative-mode MS(2) affords an easy way to

285 the quantitative comparison of site-specific glycans was achieved utilizing TMT reporter ions from HC

286 We recently reported that serum N-glycans were altered in mice displayed depressive-like b

287 70% of these N-glycans were anionic, carrying either sialic acid, sulfa

288 IgG Fc glycans were characterized in 225 healthy children and 4

289 O-Glycans were chemically released from isolated cell memb

290 Subsequently, three synthesized glycans were conjugated to DT protein to provide glycoco

291 profiles and minimal differences in IgG2 Fc glycans were noted, whereas the presence or absence of g

292 e imaged, revealing that high-mannose type N-glycans were predominantly expressed in the tumor region

293 Released O-glycans were purified by an optimized protocol to elimin

294 Cell surface O-glycans were then analyzed using a nanoLC-chip-QTOF mass

295 e modified with complex sugar structures (or glycans), which play an important role at the host-paras

296 rigin HAs contain predominately complex-type glycans, which have greater structural diversity.

297 le for generating beta1,6-branched complex N-glycans, which serve as a major ligand for this lectin.

298 Here, we engineer living cells to tag glycans with editable chemical functionalities while pro

299 tri-, and tetra-) between striatum and SN N-glycans with the detection of phosphorylated N-glycans i

300 and remove fucose from monoclonal antibody N-glycans, with significant impacts on their effector func

301 A highly conserved N-linked glycan within the IgG-Fc tail, which is essential for Ig