ライフサイエンスコーパス: glycocalyx

1 llaries from wear and infection (endothelial glycocalyx).

2 viable cell surface target within the tumor glycocalyx.

3 on epitope present on the human cell surface glycocalyx.

4 lution while sequestered within the hydrated glycocalyx.

5 nization and composition of the cell-surface glycocalyx.

6 ng of heparan fragments from the endothelial glycocalyx.

7 ranase, and hyaluronidase to the shedding of glycocalyx.

8 s ligand hyaluronan in the leukocyte surface glycocalyx.

9 nd nonsecretor antigens, distributing on the glycocalyx.

10 is and adaptive chemical modification of the glycocalyx.

11 by selective desialylation of the tumor cell glycocalyx.

12 lst depletion of ADAMTS6 induces a prominent glycocalyx.

13 ons to transfer cholesterol past a lysosomal glycocalyx.

14 lipoprotein-derived cholesterol across this glycocalyx.

15 induced damage to the glomerular endothelial glycocalyx.

16 ced disruption of the glomerular endothelial glycocalyx.

17 the predominant HS proteoglycan in the GEnC glycocalyx.

18 ription of the structure and dynamics of the glycocalyx.

19 lycoproteins that contribute to the cellular glycocalyx.

20 GEnCs expressed HA and CS in the glycocalyx.

21 king viral entry mediators on the epithelial glycocalyx.

22 iglec-E receptor protein bound to the neural glycocalyx.

23 glycocalyx is 2.2 faster than in the intact glycocalyx.

24 inds to sialic acid residues of the cellular glycocalyx.

25 iking down-regulation of components of their glycocalyx.

26 ssed as resulting from interactions with the glycocalyx.

27 s-linking transmembrane mucins on the apical glycocalyx.

28 ger 2 (AE2), and (3) an intact cholangiocyte glycocalyx.

29 lycosylation, and surface density of a model glycocalyx.

30 ed, at least in part, by the upregulation of glycocalyx.

31 degradation of, and for restoration of, the glycocalyx.

32 tional focus has extended toward endothelial glycocalyx.

33 syndecan-1, the backbone of the endothelial glycocalyx.

34 evented by repulsive forces generated by the glycocalyx, a dense and confluent layer of large and neg

35 Degradation of the endothelial glycocalyx, a glycosaminoglycan (GAG)-rich layer lining

36 are marked by degradation of the endothelial glycocalyx, a layer of glycosaminoglycans (including hep

37 To understand the function of the glycocalyx, a molecular level characterization is necess

38 The endothelial glycocalyx, a sieve-like structure located on the lumina

39 Cells are coated with a glycocalyx-a layer of carbohydrate-containing biomolecul

40 The diffusional hindrance of the glycocalyx along the cell surface on exocytotic peaks, o

41 cancer cells that express a sialic-acid rich glycocalyx also induced protease release, and this was p

42 Patients with ESLD suffer from glycocalyx alterations, and ischemia-reperfusion injury

43 ded the most reliable measure of endothelial glycocalyx anatomy, correlating with paired, numerically

44 1 inhibition restores glomerular endothelial glycocalyx and barrier function and reduces tissue infla

45 nd independently associated with endothelial glycocalyx and cell damage (ie, endotheliopathy) and fur

46 studies of the structure of the endothelial glycocalyx and dependent microvascular functions in vivo

47 erular microvascular endothelial cell (GEnC) glycocalyx and examined whether these are modified by va

48 Polysaccharides present in the glycocalyx and extracellular matrix are highly important

49 elf-enforcing feedback loop that expands the glycocalyx and furthers cancer progression.

50 that Siglec-E recognizes the intact neuronal glycocalyx and has neuroprotective function by preventin

51 or 2), the boundary covered with a layer of glycocalyx and membrane protrusions ("brush" models).

52 how they construct the extracellular complex glycocalyx and mucus is poorly understood and a future b

53 cation as well as fundamental studies of the glycocalyx and polymer physics.

54 une exclusion by trapping virions within the glycocalyx and preventing the virus from reaching immune

55 New concepts including the capillary glycocalyx and the 'baby lung' model have reshaped think

56 nt tools used to edit the composition of the glycocalyx and the future challenges in leveraging these

57 iew, we describe the basic components of the glycocalyx and the glycan moieties implicated in cancer.

58 herapeutic strategies to restore the damaged glycocalyx and to attenuate its deleterious consequences

59 tion of these cells with basement membranes, glycocalyx, and fibrils of collagen in copepods conforms

60 d' of 'solution-phase' chemokines within the glycocalyx, and that it is this soluble form of any give

61 lumen and walls of the axoneme, the membrane glycocalyx, and the intervening cytoplasm.

62 involvement in the impairment of endothelial glycocalyx are also addressed.

63 n diabetes, the size and permeability of the glycocalyx are altered.

64 sialic acid residues within the endothelial glycocalyx are critical regulators of microvascular perm

65 ymal transition (EndoMT) and shedding of the glycocalyx are early changes of endothelial dysfunction.

66 Although components of the glycocalyx are important in cell-cell interactions and o

67 aminoglycans (GAGs) as one major part of the glycocalyx are involved in many essential biological cel

68 In vertebrates, the terminal sugars of the glycocalyx are often sialic acids, 9-carbon backbone ani

69 gression, and offer a new view of the cancer glycocalyx as a major driver of disease progression.

70 We investigated the glioblastoma glycocalyx as a tumor-intrinsic immune suppressor.

71 paran sulfate, pointing to disruption of the glycocalyx as the mechanism likely mediating IS-induced

72 Systematic changes in the glycocalyx associated with specific glycoforms and glyco

73 mucin, MUC16, and a concomitant reduction in glycocalyx barrier function.

74 lammation had more significant damage to the glycocalyx barrier.

75 ysregulated cellular glucose in diabetes for glycocalyx biology and its implications for endothelial

76 bility of its sugar substrates, thus linking glycocalyx biology directly to cellular glucose metaboli

77 ovide molecular and functional insights into glycocalyx biology.

78 Increased endothelial glycocalyx breakdown is associated with severe disease a

79 Glycocalyx breakdown is increased in African children wi

80 Endothelial glycocalyx breakdown occurs in lung transplant donors an

81 Urine was assayed for glycocalyx breakdown products (glycosaminoglycans) using

82 his, we investigated the role of endothelial glycocalyx breakdown products in organ donors and recipi

83 Glycocalyx breakdown products may be useful biomarkers i

84 Endothelial glycocalyx breakdown products were measured in the perip

85 iomaterials are being developed to mimic the glycocalyx, but the spatial functionalization of these p

86 Degradation and shedding of the glycocalyx by enzymes, such as hyaluronidase 1 and hyalu

87 rs and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or di

88 l, Shurer et al. (2019) demonstrate that the glycocalyx can regulate cell shape from the outside in.

89 ucture provides visualization of an N-linked glycocalyx coat, and consideration of glycan dynamics re

90 A Sia-rich glycocalyx coats the surface of sperm, allowing them to

91 Here, we investigated the role of the glycocalyx component syndecan-1 (sdc-1) in controlling t

92 on is accompanied by shedding of one or more glycocalyx components into the blood.

93 ocal barrier of self-synthesized or acquired glycocalyx components on the macrophage surface shrouds

94 suggested differences in cancer cell-exposed glycocalyx components such as heparan sulphate.

95 ented NS1-induced degradation of endothelial glycocalyx components.

96 matory cells synthesize a rich extracellular glycocalyx composed of the chondroitin sulfate-proteogly

97 Specific glycocalyx compositions can also induce plasma membrane

98 The glycocalyx comprises glycosylated proteins and lipids an

99 s a molecularly diverse surface glycan coat (glycocalyx) comprising its interface with its cellular e

100 s, both in vivo and in vitro, show that this glycocalyx confers the ability for UMSCs to survive the

101 patients also had higher serum levels of the glycocalyx constituents hyaluronan and syndecan-1 and in

102 We hypothesized that marked shedding of the glycocalyx core protein, syndecan-1, occurs in end-stage

103 plantation, and interventions to protect the glycocalyx could improve transplant outcomes.

104 revealed that atrasentan treatment increases glycocalyx coverage in diabetic apoE KO mice from 40.7 +

105 n, however, increased glomerular endothelial glycocalyx coverage, with preservation of heparan sulfat

106 It is present in the endothelial glycocalyx covering the apical surface of endothelial ce

107 The negatively charged, brush-like glycocalyx covers the surface layer of endothelial cells

108 ine, noradrenaline), tissue/endothelial cell/glycocalyx damage (histone-complexed DNA fragments, anne

109 In addition, glycocalyx damage and vascular tone dysfunction impair m

110 grafts during preservation and the extent of glycocalyx damage correlates with the severity of hepato

111 Glycocalyx damage during I/R is evidenced by rising plas

112 ing recognized as a biomarker of endothelial glycocalyx damage in select pathologies.

113 on, the research that supports the view that glycocalyx damage is a key pathologic step in a diverse

114 ith high circulating syndecan-1 (endothelial glycocalyx damage marker) in plasma have an increased mo

115 Recipients of livers grafts with greater glycocalyx damage might be at higher risk for developmen

116 events under homeostatic conditions and how glycocalyx damage or removal will increase leukocyte adh

117 We aimed to quantify glycocalyx damage within human liver grafts after organ

118 decan-1 (Sdc-1) was measured as indicator of glycocalyx degradation in effluents of 38 liver grafts a

119 Clinical manifestations of glycocalyx degradation in unraveling acute respiratory d

120 mice rapidly induced pulmonary microvascular glycocalyx degradation via tumor necrosis factor-alpha (

121 Glycocalyx degradation was significantly associated with

122 ute coagulopathy of trauma, and endovascular glycocalyx degradation with microcirculatory compromise.

123 L secretion, thus reducing activation of the glycocalyx-degrading enzyme heparanase.

124 d, numerically smaller values of endothelial glycocalyx depth (0.078 +/- 0.016 mum) from electron mic

125 euraminidase perfusion decreased endothelial glycocalyx depth and increased apparent solute permeabil

126 A broad range of glycocalyx depth measurements (0.17-3.02 mum) were obtai

127 techniques yield measurements of endothelial glycocalyx depth that vary by over an order of magnitude

128 ensitive and reliable measure of endothelial glycocalyx depth.

129 rominent clinical situation characterized by glycocalyx derangement is ischemia-reperfusion (I/R) of

130 ar residue sialic acid of the mouse neuronal glycocalyx determines complement C1 binding and microgli

131 Our data indicate that glycocalyx differences affected the binding of cationic

132 otected against raised Ps'alb by endothelial glycocalyx disruption in glomeruli.

133 4 is likely to contribute to the endothelial glycocalyx disruption observed in diabetes and inflammat

134 d intestinal barrier function and epithelial glycocalyx disruption.

135 This review focuses on alterations of glycocalyx during I/R injury in general (to vital organs

136 st that HYAL1 contributes to endothelial and glycocalyx dysfunction induced by diabetes.

137 omedicines for many diseases associated with glycocalyx dysfunction.

138 vestigate vascular fragility and endothelial glycocalyx (ECG) in images of the healthy gingival crevi

139 Here, we report a chemoenzymatic glycocalyx editing strategy to introduce high-affinity a

140 Precision glycocalyx editing with antibody-enzyme conjugates is th

141 beneath the plasma membrane, the endothelial glycocalyx (eGC) is recognized as a vasoprotective nanob

142 om hyperbolic to sigmoidal for more rod-like glycocalyx elements.

143 polymers therefore expand the application of glycocalyx engineering in glycobiology.

144 A glycocalyx envelope consisting of proteoglycans and adhe

145 These measurements show that the glycocalyx exhibits nanoscale organization on both cell

146 tivity of orthoboric groups to diols in cell glycocalyx facilitated a rapid cross-membrane translocat

147 Our data revealed that a bulky glycocalyx facilitates integrin clustering by funnelling

148 encodes an X-linked chaperone important for glycocalyx formation, was recently identified as an IBD

149 ABSTRACT: The endothelial glycocalyx forms a continuous coat over the luminal surf

150 produce characteristic patterns of released glycocalyx fragments.

151 ely due to lateral migration of normal mucin glycocalyx from WT cells over KO crypts.

152 the roles of cell-surface carbohydrates (the glycocalyx), fundamental for cell-recognition events.

153 Endothelial glycocalyx (GCX) dysfunction may play a significant role

154 The glycocalyx (GCX), a carbohydrate-rich layer lining the e

155 wn of endomucin (EMCN), an integral membrane glycocalyx glycoprotein, prevents VEGF-induced prolifera

156 This review focuses on endothelial glycocalyx HA and highlights its key roles in the functi

157 izes the metabolic regulation of endothelial glycocalyx HA and its potential as a therapeutic target

158 Destruction of the endothelial glycocalyx has been observed within lung and kidney graf

159 ctural changes in the glomerular endothelial glycocalyx have been correlated with models of nephrotic

160 This approach enables two measurements: glycocalyx height and the distribution of individual sug

161 Additionally, we observe enhanced glycocalyx height in response to epithelial-to-mesenchym

162 Damage to endothelial glycocalyx impairs vascular barrier function and may con

163 l of atrasentan to stabilize the endothelial glycocalyx in diabetic apolipoprotein E (apoE)-deficient

164 The role of the glycocalyx in falciparum malaria and the association wit

165 introduces the theme reviews focusing on the glycocalyx in human disease.

166 or understanding the physical biology of the glycocalyx in human health.

167 In light of the importance of the glycocalyx in preserving endothelial cell integrity and

168 study to address the role of the endothelial glycocalyx in protein leakage over the glomerular filtra

169 r results suggest a fundamental role for the glycocalyx in regulating curved membrane features that s

170 the evidence for the role of the endothelial glycocalyx in regulating permeability in discrete areas

171 mortality, but the state of the endothelial glycocalyx in these patients is unknown.

172 ropose a detailed modeling framework for the glycocalyx incorporating important physical effects of b

173 The simulations show that the glycocalyx increases the effective concentration of FGFs

174 ures of sepsis as they relate to the loss of glycocalyx integrity and highlight the contribution of s

175 evaluate interstitial Na(+) and endothelial glycocalyx integrity.

176 The glycocalyx interacts directly with hemodynamic forces fr

177 The transformation of the GlcNAc "glycocalyx" into a Gal(beta1-4)GlcNAc "glycocalyx" relab

178 uronic acid (HA) is a major component of the glycocalyx involved in the vascular wall and endothelial

179 of small molecules in the partially digested glycocalyx is 2.2 faster than in the intact glycocalyx.

180 The glycocalyx is a carbohydrate-rich layer that lines the e

181 Thus, a bulky glycocalyx is a feature of tumour cells that could foste

182 The mammalian glycocalyx is a heavily glycosylated extramembrane compa

183 The endothelial surface glycocalyx is a hydrated mesh in which proteoglycans are

184 The glycocalyx is a layer coating the luminal surface of vas

185 The glycocalyx is a layer of glycoaminoglycans, proteoglycan

186 to a growing body of evidence that the tumor glycocalyx is a promising target for synthetic immunothe

187 The glycocalyx is a sugar-rich layer located at the luminal

188 The glycocalyx is a thick coat of proteins and carbohydrates

189 The glycocalyx is a thin layer of glycolipids, glycoproteins

190 The endothelial glycocalyx is a vital regulator of vascular permeability

191 Because the endothelial glycocalyx is also reduced in diabetic nephropathy, we h

192 The endothelial glycocalyx is critically involved in vascular integrity

193 Our data suggest that the glycocalyx is damaged within human liver grafts during p

194 other pathologic conditions, the endothelial glycocalyx is damaged, and its degradation is accompanie

195 ls will be less dependent upon NPC1 if their glycocalyx is decreased in density.

196 The turnover of the glycocalyx is dynamic to fine-tune these processes.

197 Degradation of the glycocalyx is now considered a cornerstone in I/R-relate

198 ctural integrity of a glomerular endothelial glycocalyx is required to prevent albuminuria.

199 Motility assays demonstrate that as their glycocalyx is shed in the female's sperm storage organs,

200 ecognition events, a fundamental role of the glycocalyx is the inhibition of nonspecific interactions

201 Characterization of the glycocalyx is thus essential to understanding cell physi

202 ey roles in the functions of the endothelial glycocalyx, its shedding in several pathologic condition

203 The anatomical distance between maximal glycocalyx label and maximal endothelial cell plasma mem

204 distance between peak cell membrane and peak glycocalyx label provided the most reliable measure of e

205 th fluorescent endothelial cell membrane and glycocalyx labels, and imaged with confocal microscopy.

206 the adjusted association of the endothelial glycocalyx layer (EGL) and tight and adherens junction m

207 O production; degradation of the endothelial glycocalyx layer (EGL) drastically reduces EC production

208 The endothelial glycocalyx layer (EGL), which consists of long proteogly

209 To explore the role of the glycocalyx layer of the endothelial extracellular matrix

210 short-term loss of integrity of the luminal glycocalyx layer resulted in perturbed glomerular filtra

211 esigned a simple coarse-grained model of the glycocalyx layer, or adhesive mucus layer (AML), covered

212 main structural component of the endothelial glycocalyx layer.

213 establish a framework for understanding how glycocalyx layers with different thickness and stiffness

214 ctionated heparin chains, coats cells with a glycocalyx-like layer and may inhibit (xeno)transplant-a

215 igands presented at the nanoscale within the glycocalyx mimicking layers on surfaces.

216 troduce the most detailed, to-date, all-atom glycocalyx model, composed of lipid bilayer, proteoglyca

217 ibes how to comprehensively characterize the glycocalyx N-glycans and O-glycans of glycoproteins, as

218 such as the actin-cytoskeleton network, the glycocalyx network, and nonequilibrium transport under A

219 Structural and functional damage to glycocalyx occurs in various disease states.

220 The pronounced glycocalyx of bladder cancer cells enhanced the internal

221 However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding

222 lected huNoV-like particles (VLPs) bound the glycocalyx of HIOs with matched HBGA phenotypes.

223 ecently shown that MUC16, a component of the glycocalyx of some mucosal barriers, has elevated bindin

224 ngly suggest a substantial remodeling of the glycocalyx of spermatozoa and epididymal epithelial cell

225 The glycocalyx of the cell is composed of highly hydrated sa

226 Our results indicate that the glycocalyx of tumour cells controls the binding and biol

227 tructural components (connective tissue, CT; glycocalyx) of donor organs, and engineering of new orga

228 cosylated glycoproteins that coat the apical glycocalyx on mucosal surfaces and represent the first l

229 e were no changes in measures of endothelial glycocalyx or microvascular function.

230 tion and preceded changes in the endothelial glycocalyx or microvascular function.

231 Endothelial glycocalyx participates in the maintenance of vascular i

232 This study shows that the glycocalyx plays an important role in the diffusion kine

233 Next, we examine the important role the glycocalyx plays in driving tension-mediated cancer cell

234 hts into the dynamic remodeling of the sperm glycocalyx prior to fertilization.

235 stigating whether bulky glycoproteins in the glycocalyx promote a tumour phenotype in human cells by

236 Here we report that a bulky glycocalyx promotes the expansion of disseminated tumor

237 se expression in the podocyte, damage to the glycocalyx, proteinuria, and renal failure.

238 IAV infection, and therefore this synthetic glycocalyx provides a useful reductionist model for stud

239 ns in particular), and on maneuvers aimed at glycocalyx recovery during I/R injury.

240 Sialic acid residues of the endothelial glycocalyx regulate glycocalyx structure and microvessel

241 The endothelial glycocalyx regulates blood vessel permeability and homeo

242 The glycocalyx regulates the interaction of mammalian cells

243 Glycocalyx regulation of vascular endothelial growth fac

244 cNAc "glycocalyx" into a Gal(beta1-4)GlcNAc "glycocalyx" relabeled these vesicles, making them suscep

245 sociated with degradation of the endothelial glycocalyx, releasing heparan sulfate fragments (of suff

246 e in both health and disease, studies of the glycocalyx remain hampered by a paucity of methods to sp

247 This glycocalyx remodeling strategy is versatile and may be a

248 s were observed following removal of the ECM/glycocalyx, replacement of these glycosylated asparagine

249 Furthermore, we study the glycocalyx response under shear flow and its role as a f

250 hed endothelial GAG network (the endothelial glycocalyx) results in increased vascular resistance and

251 min) and mitochondria (from 6 h) along with glycocalyx shedding (from 6 h), which were blocked by pr

252 rix metalloproteinase, alleviated UA-induced glycocalyx shedding and EndoMT.

253 aluated the effects of general anesthesia on glycocalyx shedding and its association with acute kidne

254 Volatile anesthetics did not attenuate glycocalyx shedding in human OLT.

255 iorated EndoMT; however, they did not change glycocalyx shedding in HUVECs.

256 cells via induction of oxidative stress with glycocalyx shedding, which could be one of the mechanism

257 cells via induction of oxidative stress and glycocalyx shedding.

258 hat lack the major anionic components of the glycocalyx, sialic acid and glycosaminoglycans, and in c

259 dothelium-dependent vasodilation, arteriolar glycocalyx size, and glomerular barrier properties in wi

260 al for the constitution of the cone-specific glycocalyx stained by the PNA (peanut agglutinin) lectin

261 as been claimed to represent the endothelial glycocalyx state of health.

262 ing cases for the structural elements of the glycocalyx: stiff beams and flexible chains.

263 including hyaluronic acid and mucins, in the glycocalyx strongly correlates with the aggression of ma

264 l-time in vivo quantification of endothelial glycocalyx structure and associated microvessel permeabi

265 increases glycocalyx thickness and maintains glycocalyx structure and HA content during diabetes; and

266 idues of the endothelial glycocalyx regulate glycocalyx structure and microvessel permeability to bot

267 in adult mice led to substantial loss of the glycocalyx structure, and analysis of their kidneys and

268 A polymer brush model of the glycocalyx successfully predicts the effects of polymer

269 s are more readily trapped by the glomerular glycocalyx than larger ones.

270 ngagement with hyaluronan assemblages in the glycocalyx that are large enough to cross-bridge the cor

271 Lysosomes are lined with a glycocalyx that protects the limiting membrane from the

272 tend to accumulate upstream of the podocyte glycocalyx that spans the slit, but none are observed up

273 ed mediator of tumor-ECM interactions is the glycocalyx, the sugar-decorated proteins and lipids that

274 ase 2, are responsible for regulation of the glycocalyx thickness and hence access of circulating cel

275 (EDH)-mediated vasorelaxation; 2) increases glycocalyx thickness and maintains glycocalyx structure

276 othelial heparanase expression and increased glycocalyx thickness in the presence of a diabetic milie

277 n heparan sulfate expression and endothelial glycocalyx thickness, and development of proteinuria obs

278 pecific epitope responses to localize to the glycocalyx through interaction with this specific mucin.

279 cells (ECs), previous studies have shown the glycocalyx to be a significant mediator of NO production

280 tions predict the mechanical response of the glycocalyx to compressive loads, which are imposed on ce

281 acellular forces are transmitted through the glycocalyx to initiate intracellular signaling pathways.

282 flu pathogenesis, we constructed a synthetic glycocalyx to investigate membrane-tethered mucins in th

283 of individual components of the endothelial glycocalyx to one critical vascular function, microvascu

284 ution of sialic acids within the endothelial glycocalyx to the structural and functional permeability

285 ld concentrate certain antibodies within the glycocalyx to trap pathogens before they can reach the u

286 measure the contribution of the endothelial glycocalyx to vessel wall permeability.

287 the lamina densa of the GBM and the podocyte glycocalyx, together with saturable tubular capture, det

288 sialic acid residues within the endothelial glycocalyx using neuraminidase perfusion decreased endot

289 r-infrared spectroscopy, and the endothelial glycocalyx was assessed by sublingual videomicroscopy an

290 An apical glycocalyx was identified on cholangiocytes in vitro by

291 y injured endothelium at sites where surface glycocalyx was reduced.

292 Inspired by glycoproteins present in the glycocalyx, we describe a new class of synthetic antifou

293 protective role of the cholangiocyte apical glycocalyx, wherein disruption of the glycocalyx with ne

294 umors have an increase in the density of the glycocalyx, which in turn increases the tension of the c

295 resence of repulsive polymers that mimic the glycocalyx, which points to two potential mechanisms for

296 a 110-nm-thick layer and even further for a glycocalyx with larger thickness.

297 apical glycocalyx, wherein disruption of the glycocalyx with neuraminidase increased the permeability

298 Partial digestion of the glycocalyx with neuraminidase led to the observation of

299 Tumor cells express a unique cell surface glycocalyx with upregulation of sulfated glycosaminoglyc

300 that sperm shed their entire outer coat, the glycocalyx, within 24 hours of being stored in the femal