ライフサイエンスコーパス: glyoxalase I

1 tivity relevant to Ni(II)-containing E. coli glyoxalase I.

2 planation for the stereochemical behavior of glyoxalase I.

3 ve inhibitor of the anticancer target enzyme glyoxalase I.

4 inhibit the methylglyoxal-detoxifying enzyme glyoxalase I.

5 ate that forms along the reaction pathway of glyoxalase I.

6 C is diminished in the absence of functional glyoxalase I.

7 ay for MG in E. coli and that the product of glyoxalase I activity, S-lactoylglutathione, is the acti

8 Glyoxalase-I activity in these cells was increased 28-fo

9 To evaluate directly the effect of glyoxalase-I activity on intracellular AGE formation, GM

10 Glyoxalase I alone among the core MG protective systems

11 ncient betaalphabetabetabeta fold motif with glyoxalase I and bleomycin resistance protein families,

12 is of published values for the activities of glyoxalase I and glyoxalase II in lysed erythrocytes and

13 MG is detoxified by glyoxalase I and II family proteins in plants.

14 Comparison with related enzymes (glyoxalase I and MnSOD) suggests that the change in the

15 or detoxification of reactive electrophiles: glyoxalase I and N-ethylmaleimide reductase.

16 ied proteins, whereas other targets, such as glyoxalase I and ribose 5-phosphate isomerase, detoxify

17 elated to at least two other metalloenzymes, glyoxalase I and the Mn2+- or Fe2+-containing extradiol

18 The structure of the active site of human glyoxalase I and the reaction mechanism of the enzyme-ca

19 Glyoxalase-I and gamma-glutamyl transpeptidase, both inv

20 strains contained the glutathione-dependent glyoxalases I and II, as well as the glutathione-indepen

21 Thus, glyoxalase I appears to be a novel example of a single p

22 ites of human, yeast, and Pseudomonas putida glyoxalase I, as the log K(i) values for these mechanism

23 llographic measurements, indicate that human glyoxalase I binds the syn-conformer of S-(N-aryl-N-hydr

24 For the glyoxalase-I-catalyzed isomerization of glutathione (GSH

25 Glyoxalase I catalyzes still another glutathione-depende

26 Glyoxalase-I catalyzes the conversion of MG to S-D-lacto

27 Overexpression of glyoxalase-I completely prevented both hyperglycemia-ind

28 s that are catalyzed include isomerizations (glyoxalase I), epimerizations (methylmalonyl-CoA epimera

29 Cells overexpressing glyoxalase I exhibit enhanced tolerance of methylglyoxal

30 um-1,2-diolate) , we observed both decreased glyoxalase I expression and activity relative to untreat

31 (GSH/GSSG) ratio through the upregulation of glyoxalase I expression, and resists oxidative stress.

32 nct superfamily of metalloenzymes containing glyoxalase I, extradiol dioxygenases, and methylmalonyl-

33 The glyoxalase I gene (gloA) of Escherichia coli has been cl

34 tion screening, we found that mutations in a glyoxalase I gene (named GERMINATION-IMPAIRED GLYOXALASE

35 ut we do find Alkbh7(-/-) mice have elevated glyoxalase I (GLO-1), a dialdehyde detoxifying enzyme.

36 olesterol-related genes, but also pointed to glyoxalase I (GLO1) as a gene implicated in cholesterol

37 on is performed by a trypanothione-dependent glyoxalase I (GLO1) containing a nickel cofactor; all ot

38 n of the methylglyoxal detoxification enzyme glyoxalase I (Glo1) potentiates methylglyoxal sensitivit

39 say of MGS by coupling of the MG produced to glyoxalase I (Glx I): MG + glutathione (GSH) --> (S)-lac

40 The BSH-dependent pathway utilizes glyoxalase I (GlxA, formerly YwbC) and glyoxalase II (Gl

41 The metalloenzyme glyoxalase I (GlxI) converts the nonenzymatically produc

42 Escherichia coli glyoxalase I (GlxI) is a metalloisomerase that is maxima

43 ed to study the catalytic mechanism of human glyoxalase I (GlxI).

44 Glyoxalase I (GLYI) constitute the first enzyme of the g

45 domain-swapped dimer but Pseudomonas putida glyoxalase I has been reported to be monomeric.

46 Human glyoxalase I has the structure of a domain-swapped dimer

47 The structures of human glyoxalase I in complexes with S-(N-hydroxy-N-p-iodophen

48 Overexpression of glyoxalase I in HRP protected against S-p-bromobenzylglu

49 the role of MGO and its metabolizing enzyme, glyoxalase I, in high glucose-induced apoptosis (annexin

50 c complexicity that have plagued the area of glyoxalase I inhibition.

51 se in the intracellular concentration of the glyoxalase I inhibitor (kapp = 1.41 +/- 0.03 min-1 (37 d

52 thylglyoxal metabolism with a cell permeable glyoxalase I inhibitor provoked these responses in normo

53 (N-aryl-N-hydroxycarbamoyl)glutathione-based glyoxalase I inhibitors culminated in the discovery of t

54 We show here that recombinant P. putida glyoxalase I is an active dimer (kcat approximately 500

55 Our findings indicate that glyoxalase I is critical for pericyte survival under hyp

56 data suggest that the glutathione-dependent glyoxalase I is the dominant detoxification pathway for

57 Glyoxalase-I is a glutathione-binding protein involved i

58 or the more active H-2d-linked allele at the Glyoxalase I locus.

59 Knowledge regarding the regulation of glyoxalase-I may provide insights into the importance of

60 hibition is due to competitive inhibition of glyoxalase I, preincubation of L1210 cells with 2(Et)2 i

61 on-dependent kinetic properties of the yeast glyoxalase I reaction have been measured by means of vis

62 e quantum motif for the investigation of the glyoxalase I reaction.

63 of the enzyme and about the mechanism of the glyoxalase I reaction.

64 To gain a better understanding of the glyoxalase-I regulation under normal physiological condi

65 lopentyl diester, a competitive inhibitor of glyoxalase I, resulted in apoptosis along with a dramati

66 In contrast, in glyoxalase-I-transfected cells, 30 mM glucose incubation

67 energies of Co(2+) and Ni(2+) to the enzyme glyoxalase I using an optimized 12-6-4 (m12-6-4) potenti

68 endothelial cells that stably express human glyoxalase-I were generated.