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2 e detected in diverse biological fluids from gnotobiotic and conventionally colonized mice and traced
3 ora in immunocompetent animals, we generated gnotobiotic and conventionally reared Ig allotype chimer
5 t and a model of Giardia mono-association in gnotobiotic and immunodeficient mice to propose an alter
6 ow in multiple specific pathogen-free (SPF), gnotobiotic, and germ-free murine models of GI GVHD that
7 iew summarizes the results of developmental, gnotobiotic, and in vitro studies that showed alteration
10 roughput anaerobic culturing techniques with gnotobiotic animal husbandry and metagenomics to show th
11 responses are experimentally delineated via gnotobiotic animal models or alternatively by antibiotic
12 ment microbiota from trial participants into gnotobiotic animals and re-enacting trial conditions all
14 and the latest progress in using axenic and gnotobiotic approaches to study insect-microbe interacti
15 and survival of congenitally immunodeficient gnotobiotic beige-athymic (bg/bg-nu/nu) and beige-euthym
16 Earlier results showed that conventional and gnotobiotic but not axenic larvae exhibit midgut hypoxia
18 postinfection, all H. saguini-monoassociated gnotobiotic C57BL/129 IL-10(-/-) mice were colonized and
19 undred seventy-nine mice including C57BL/6J, gnotobiotic C57BL/6J, and knockout strains for GPR41, GP
20 n orally inoculated into a BCoV-seronegative gnotobiotic calf, GiCoV-OH3 caused severe diarrhea and v
21 nomes from both the cell culture-adapted and gnotobiotic-calf-passaged strains were also sequenced an
22 gainst the virulent enteric BCoV DB2 strain, gnotobiotic calves (n = 4) were orally inoculated with H
23 e some differences of degree, all inoculated gnotobiotic calves (n = 6) showed abnormal feces between
25 ervations described previously in studies of gnotobiotic calves and pigs experimentally infected with
31 -free male and female MCAD(-/-) mice enabled gnotobiotic colonization combined with untargeted metabo
33 sequencing, in vitro functional assays, and gnotobiotic colonizations to define the microbial compos
34 ifestations under specific-pathogen-free and gnotobiotic conditions, notably increasing plasmacytoid
45 we colonize mice that are maintained in the gnotobiotic facility with O. formigenes, using either a
48 these interactions involved colonization of gnotobiotic Fut2(+) and Fut2(-) mice with Bacteroides th
50 such colonization by using antimicrobials or gnotobiotic germ-free mice overrides these protective be
55 idney cell line, and the WT PEC, passaged in gnotobiotic (Gn) pigs, were used to orally inoculate 13
58 pression in buccal and intestinal tissues of gnotobiotic (Gn) pigs; (ii) to determine if virus-like p
61 e degree of gastrointestinal inflammation in gnotobiotic HLA-B27 transgenic rats monoassociated with
64 study, we demonstrate that immunosuppressed gnotobiotic (IGB) piglets orally inoculated with wild-ty
71 onic acid, colitis induced by microflora (in gnotobiotic interleukin-10(-/-)), and colitis induced by
74 howed that bacteria in nonsterile larvae and gnotobiotic larvae inoculated with wild-type E. coli red
76 s the growth of only E. coli-to bi-colonized gnotobiotic mice abolished the colonization-resistance c
77 the underlying mechanisms, we introduce into gnotobiotic mice an artificial community composed of hum
78 d metabolites that circulate in the blood of gnotobiotic mice and are also detected in plasma from he
80 study UTI89 recovered from the distal gut of gnotobiotic mice and from IBCs harvested by laser captur
81 SAM and MAM microbiota were characterized in gnotobiotic mice and gnotobiotic piglets colonized with
82 tryptophanase, we can modulate IS levels in gnotobiotic mice and in the background of a conventional
85 bicans depleted simple sugars in the ceca of gnotobiotic mice but required oxygen to grow on these re
86 position of a diverse synthetic community in gnotobiotic mice can be quantitatively predicted from ba
88 lico, and using sequencing data derived from gnotobiotic mice colonized with a synthetic fecal microb
90 in vitro during growth in rich medium and in gnotobiotic mice colonized with defined communities of h
92 and lactulose increased luminal butyrate in gnotobiotic mice colonized with feces from an allergic i
93 in the gut of germ-free mice and restored in gnotobiotic mice colonized with members of the classes B
95 on sialylated substrates, we showed that-in gnotobiotic mice colonized with R. gnavus wild-type (WT)
96 n bacterial membranes, experiments employing gnotobiotic mice colonized with recombinant PG overprodu
99 n following Plasmodium infection compared to gnotobiotic mice colonized with the fecal samples of mal
100 onally, orally administering glycan beads to gnotobiotic mice confirmed specificity in glycan binding
101 eneChips to characterize their expression in gnotobiotic mice consuming polysaccharide-rich or -defic
102 We then show MDSINE's utility on two new gnotobiotic mice datasets, investigating infection with
105 gut bacterial strains introduced into adult gnotobiotic mice fed a polysaccharide-rich diet, and (ii
106 these fungal species on metabolic health in gnotobiotic mice fed standard (SD) or high-fat-high-sucr
107 ad SG preparation was orally administered to gnotobiotic mice harboring a consortium of 56 cultured,
108 matexigens in vitro and in the intestines of gnotobiotic mice harboring a prominent saccharolytic bac
110 re most obvious in the gut, where studies of gnotobiotic mice have disclosed that the microbiota affe
111 hich human gut communities are replicated in gnotobiotic mice have provided an opportunity to identif
112 Unique signatures of in vivo competition in gnotobiotic mice include an adhesin enriched in poor col
114 cation for controlling the gut microbiota of gnotobiotic mice infected with Clostridium difficile and
119 ransfer of a hypertensinogenic microbiota to gnotobiotic mice recapitulated the prebiotic-deprived hy
121 thetaiotaomicron population dynamics within gnotobiotic mice reveal that these populations are relat
122 analysis of intestinal epithelial cells from gnotobiotic mice revealed a previously unidentified mech
123 ota samples and purified fecal VLPs from the gnotobiotic mice revealed a reproducible nonsimultaneous
125 nd reuniting them in various combinations in gnotobiotic mice should facilitate preclinical studies d
127 to experiments in conventionally raised and gnotobiotic mice suggest that variations in gut microbia
129 M. smithii's transcriptome and metabolome in gnotobiotic mice that do or do not harbor Bacteroides th
130 eropathy of the small intestine developed in gnotobiotic mice that had been colonized with cultured d
131 file of C. difficile within the intestine of gnotobiotic mice to identify genes regulated in response
132 y underpinning such treatments, we have used gnotobiotic mice to model microbiota manipulations in th
133 species, were introduced simultaneously into gnotobiotic mice together with 11 other wild-type strain
140 epithelial progenitors (GEPs) in transgenic gnotobiotic mice with a ChAG-like phenotype harbor intra
145 les and microbial transplantation studies in gnotobiotic mice with defined synthetic communities show
146 les and microbial transplantation studies in gnotobiotic mice with defined synthetic communities show
147 Furthermore, we show that colonization of gnotobiotic mice with purine-degrading bacteria modulate
148 erm-free, deliberately colonized adult mice (gnotobiotic mice) were used to examine the efficacy of c
149 ing >90,000 isogenic transposon mutants into gnotobiotic mice, along with the other artificial commun
151 enced human gut bacteria was introduced into gnotobiotic mice, and changes in species abundance and m
152 odulate serum levels of these metabolites in gnotobiotic mice, and show that in turn this affects int
153 immunocompetent and defined immunodeficient gnotobiotic mice, by reverse-transcription polymerase ch
154 rocessed foods affect the microbiota, we fed gnotobiotic mice, colonized with 54 phylogenetically div
157 ences in beta -defensin expression in gf and gnotobiotic mice, they also suggest a role for these pep
158 ic pathways, some of which were confirmed in gnotobiotic mice, together with observed changes in the
160 ges across four human Bacteroides strains in gnotobiotic mice, we observed positive selection on thou
176 The generation of axenic (germ-free) and gnotobiotic model systems has been vital to dissecting t
177 microbiota and immune system, we employed a gnotobiotic model using human fecal transfers into ATG16
178 tudies of host-microorganism interactions in gnotobiotic models and in vitro systems, to molecular me
179 Combining antigenically defined diets with gnotobiotic models, we show that food and microbiota dis
181 NMRI mice were colonized at birth from their gnotobiotic mothers, who harbored this anaerobic Gram-ne
184 demonstrate that initial colonization of the gnotobiotic mouse intestine by B. fragilis requires that
186 and promoted MDCF-dependent weight gain in a gnotobiotic mouse model emulating the clinical trials.
187 act to regulate gut motility, we developed a gnotobiotic mouse model that mimics short-term dietary c
195 rthermore, the use of genetically engineered gnotobiotic mouse models may increase our understanding
198 a on enteric-associated neurons by combining gnotobiotic mouse models with transcriptomics, circuit-t
201 BSH enzymes in the gastrointestinal tract of gnotobiotic or conventionally raised mice significantly
202 rus vaccination regimens were evaluated in a gnotobiotic pig model of rotavirus infection and disease
206 ere constructed and subsequently included in gnotobiotic piglet challenge studies, and their pathogen
214 filaments in the tongues of immunosuppressed gnotobiotic piglets and when embedded in agar, demonstra
215 up population dynamics were recapitulated in gnotobiotic piglets as they transitioned from exclusive
216 a were characterized in gnotobiotic mice and gnotobiotic piglets colonized with age- and growth-discr
217 y, we evaluated the efficacy of MMV665917 in gnotobiotic piglets experimentally infected with Cryptos
218 e virulence of these strains was compared in gnotobiotic piglets expressing receptors for F4(+) fimbr
220 cted mice challenged with Stx2 and protected gnotobiotic piglets infected with STEC from fatal system
222 cked-cell volume and plasma total protein of gnotobiotic piglets inoculated with the LT-positive stra
224 ions or symptoms developed in 18 (90%) of 20 gnotobiotic piglets orally infected with strain 86-24, i
227 cytotoxin in the pathogenesis of gastritis, gnotobiotic piglets were colonized with either toxigenic
232 e orally inoculated five groups of 5-day-old gnotobiotic piglets with the three mutants, icPC22A, or
233 owever, in contrast to previous studies with gnotobiotic piglets, there was no evidence that the expr
244 in serum and intestinal contents of neonatal gnotobiotic pigs and IL-12, IFN-gamma, IL-4, and IL-10 c
246 n RV (AttHRV) and virulent human RV (HRV) in gnotobiotic pigs colonized with probiotics (Lactobacillu
250 inoculated pigs was confirmed by inoculating gnotobiotic pigs orally with pooled HRV-positive serum.
251 ptibility to human rotavirus (HRV) diarrhea, gnotobiotic pigs provide a useful model for rotaviral di
252 hese results suggest that GE immunodeficient gnotobiotic pigs serve as a novel model for biomedical r
253 evel serum and mucosal antibody responses in gnotobiotic pigs than those induced by the tissue cultur
254 pe 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuG1 or BoG2 oocysts.
258 ate that 2/6-VLP vaccines are immunogenic in gnotobiotic pigs when inoculated i.n. and that the adjuv
260 HuNoV infects B cells in vivo, we colonized gnotobiotic pigs with E. cloacae and inoculated pigs wit
261 in addition to gastrointestinal infection in gnotobiotic pigs, confirming previous reports of rotavir
263 r respiratory tract infections or viremia in gnotobiotic pigs, we inoculated them with attenuated or
264 lls were not a target cell type for HuNoV in gnotobiotic pigs, with or without E. cloacae colonizatio
271 treptococcus mutans increases virulence in a gnotobiotic rat model and also promotes in vivo accumula
272 stinguishable from its wild-type parent in a gnotobiotic rat model of caries but was significantly le
275 g gut-resident microorganisms and of rearing gnotobiotic rodents have made it possible to assess the
276 re limited to T follicular helper cells in a gnotobiotic setting, without appreciable induction of ot
279 Importantly, the development of several gnotobiotic systems is allowing causative and mechanisti
281 We combined gut commensal genetics with gnotobiotics to measure brivudine drug metabolism across
282 produced a fitness defect in the stomachs of gnotobiotic transgenic mice but not in wild-type litterm
284 e cancer-associated strain was less fit in a gnotobiotic transgenic mouse model of human ChAG and bet
285 tropism of H. pylori clinical isolates in a gnotobiotic transgenic mouse model of human chronic atro
292 rescent fatty acid (FA) analogs delivered to gnotobiotic zebrafish hosts, we reveal that microbiota s
293 These results demonstrate the utility of gnotobiotic zebrafish in defining the behavior and local
296 perimental evolution of Aeromonas veronii in gnotobiotic zebrafish, we identify bacterial traits prom