ライフサイエンスコーパス: guanylyltransferase

1 nct from the eukaryotic mRNA capping enzyme, guanylyltransferase.

2 ment in the binding of CaCet1p to the fungal guanylyltransferase.

3 n complex by linkage in cis to the mammalian guanylyltransferase.

4 hat the LEF-4 subunit of RNA polymerase is a guanylyltransferase.

5 enesis of Ceg1, the Saccharomyces cerevisiae guanylyltransferase.

6 ed structure as is formed by protein GTP:RNA guanylyltransferase.

7 inactivated the triphosphatase, but not the guanylyltransferase.

8 e capping enzymes RNA triphosphatase and RNA guanylyltransferase.

9 from that of eukaryotic mRNA capping enzyme, guanylyltransferase.

10 rase and also encodes the RNA capping enzyme guanylyltransferase.

11 iB synthetase, and to AdoCbi-GDP by the CobY guanylyltransferase.

12 h are catalyzed by an RNA triphosphatase and guanylyltransferase.

13 on factor-1alpha, heat-shock protein 60, and guanylyltransferase.

14 tains a submolar amount of cellular mRNA cap guanylyltransferase.

15 nd found that it encodes mannose-1-phosphate guanylyltransferase.

16 in the binding of Schizosaccharomyces pombe guanylyltransferase.

17 hate end that is then capped with GMP by RNA guanylyltransferase.

18 unction in concert with the endogenous yeast guanylyltransferase.

19 ided that it is coexpressed with the S.pombe guanylyltransferase.

20 s with the inherent thermal stability of the guanylyltransferase.

21 d in yeast and vaccinia virus capping enzyme guanylyltransferases.

22 activating mutation (K294A) of the mammalian guanylyltransferase active site in the fusion protein ha

23 es the phosphohydrolase active site from the guanylyltransferase active site.

24 and catalysis at both the triphosphatase and guanylyltransferase active sites.

25 nds on a bifunctional enzyme with kinase and guanylyltransferase activities (CobP in aerobic adenosyl

26 ns both the N7-guanine methyltransferase and guanylyltransferase activities and catalyzes the 5' end

27 quential action of RNA 5'-triphosphatase and guanylyltransferase activities in the bifunctional mamma

28 ucleoside triphosphate phosphohydrolase, and guanylyltransferase activities of the vaccinia virus mRN

29 Measurement of the relative ATPase and guanylyltransferase activities remaining in D1R carboxyl

30 zyme TbCe1 which possesses an RNA kinase and guanylyltransferase activities that can convert decapped

31 ossessing ATPase, RNA 5'-triphosphatase, and guanylyltransferase activities, was expressed in Escheri

32 acid protein with RNA triphosphatase and RNA guanylyltransferase activities.

33 proteins with both RNA 5'-triphosphatase and guanylyltransferase activities.

34 bifunctional domain with triphosphatase and guanylyltransferase activities.

35 regard to ATPase, RNA 5'-triphosphatase, and guanylyltransferase activities.

36 mRNA capping enzyme with triphosphatase and guanylyltransferase activities.

37 mRNA capping enzyme with triphosphatase and guanylyltransferase activities.

38 nces the stimulation of human capping enzyme guanylyltransferase activity and RNA cap formation by tr

39 o alanine caused only a partial reduction in guanylyltransferase activity at the autoguanylylation st

40 enzyme had GTP:adenosylcobinamide-phosphate guanylyltransferase activity but did not have the NTP:Ad

41 CTD peptides containing Ser-5-PO4 stimulate guanylyltransferase activity by enhancing enzyme affinit

42 1, but it also allosterically activates Ceg1 guanylyltransferase activity in the context of Pol II bi

43 of the effects of motif VI mutations on Mce1 guanylyltransferase activity in vitro highlights essenti

44 ammalian reovirus particles and contains the guanylyltransferase activity involved in adding 5' caps

45 Here we show that the guanylyltransferase activity of Ceg1 is highly thermolab

46 ith Ceg1 elicits >10-fold stimulation of the guanylyltransferase activity of Ceg1.

47 Cet1p binding to Ceg1p stimulates the guanylyltransferase activity of Ceg1p.

48 sphorylation of CTD by P-TEFb stimulates the guanylyltransferase activity of human capping enzyme and

49 n had no significant effect on the ATPase or guanylyltransferase activity of LEF-4 but resulted in a

50 le for enhancing both the RNA kinase and the guanylyltransferase activity of TbCe1.

51 first inhibitor that targets the GTP-binding/guanylyltransferase activity of the flavivirus RNA cappi

52 st, N6 methyladenosine severely inhibits the guanylyltransferase activity of the mammalian capping en

53 king to SAM but had no effects on either the guanylyltransferase activity of this protein or its conf

54 performed with open cores indicates that the guanylyltransferase activity of VP3 is nonspecific and i

55 e Cet1-Ceg1 interaction is to stabilize Ceg1 guanylyltransferase activity rather than to allosterical

56 the guanylyltransferase domain abolished the guanylyltransferase activity without affecting triphosph

57 eterminant of CTD binding and stimulation of guanylyltransferase activity, and of Mce function in viv

58 The LEF-4 subunit has guanylyltransferase activity, suggesting that baculoviru

59 ears to be both necessary and sufficient for guanylyltransferase activity.

60 eptide containing Ser-2-PO4 has no effect on guanylyltransferase activity.

61 ions at these sites retained binding but not guanylyltransferase activity.

62 mRNA capping enzyme (Mce1) and stimulate its guanylyltransferase activity.

63 The 5' cap, catalyzed by RNA guanylyltransferase and 5'-phosphatase (RNGTT), is a vit

64 The N-terminal domain of DENV NS5 has guanylyltransferase and methyltransferase (MTase), and t

65 the rotavirus VP3 enzyme, which encodes both guanylyltransferase and methyltransferase activities, is

66 The fungal guanylyltransferase and methyltransferase are structural

67 additional inserted domain, and a C-terminal guanylyltransferase and RNA 5'-triphosphatase domain.

68 In addition to TbCe1 guanylyltransferase and TbCmt1 (guanine N-7) methyltrans

69 tational studies of Saccharomyces cerevisiae guanylyltransferase and the crystal structures of Chlore

70 hosphorylation-dependent interaction between guanylyltransferase and the CTD is conserved from yeast

71 t has been proposed to function as the viral guanylyltransferase and to direct the capping of the 11

72 Tat stimulates the guanylyltransferase and triphosphatase activities of Mce

73 rs on the structure of murine capping enzyme guanylyltransferase and yeast studies of the recognition

74 the sequential action of RNA triphosphatase, guanylyltransferase, and (guanine-N-7)methyltransferase.

75 on of three enzymes: RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7)-methyltransfera

76 ctional protein with RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7-) methyltransfer

77 ast, Schizosaccharomyces pombe and mammalian guanylyltransferases are intrinsically thermostable in v

78 Guanylyltransferases are members of the nucleotidyltrans

79 the separate tunnel-type triphosphatase and guanylyltransferase as the aboriginal state of the cappi

80 Here we localize the guanylyltransferase-binding and guanylyltransferase-stim

81 The guanylyltransferase-binding domain is located on the pro

82 of a WAQKW motif within this segment abolish guanylyltransferase-binding in vitro and Cet1p function

83 We report that mammalian guanylyltransferase binds synthetic CTD peptides contain

84 nding and allosteric activation of mammalian guanylyltransferase by CTD Ser5-PO4, whereas alanine mut

85 This finding illustrates that mammalian guanylyltransferase can be used as a vehicle to deliver

86 lata, we have characterized and purified the guanylyltransferase (capping enzyme), which transfers GM

87 ed by the ceg1-25 mutation of the yeast mRNA guanylyltransferase (capping enzyme).

88 nit contains the signature motifs of GTP:RNA guanylyltransferases (capping enzymes).

89 al and bacterial NLA pathways, two different guanylyltransferases catalyze the activation of the corr

90 l in vivo in yeast in lieu of the endogenous guanylyltransferase Ceg1; (iii) the guanylyltransferase

91 cerevisiae RNA triphosphatase (Cet1) and RNA guanylyltransferase (Ceg1) interact in vivo and in vitro

92 unoblotting suggests that the capping enzyme guanylyltransferase (Ceg1) is stabilized in vivo by its

93 consists of separate triphosphatase (Cet1), guanylyltransferase (Ceg1), and methyltransferase (Abd1)

94 an RNA 5'-triphosphatase (Cet1) and an mRNA guanylyltransferase (Ceg1).

95 he RNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1).

96 e mRNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1).

97 of the methyltransferase, Abd1, but not the guanylyltransferase, Ceg1, suggesting that Abd1 contribu

98 t1(231-549)p binds in vitro to the yeast RNA guanylyltransferase Ceg1p to form a 7.1 S complex that w

99 erevisiae RNA triphosphatase (Cet1p) and RNA guanylyltransferase (Ceg1p) interact in vivo and in vitr

100 isting of RNA 5'-triphosphatase (Cet1p), RNA guanylyltransferase (Ceg1p), and Abd1p could be replaced

101 endogenous fungal triphosphatase (Cet1p) and guanylyltransferase (Ceg1p).

102 The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 cocrystallized with a carboxy-t

103 Candida albicans guanylyltransferase Cgt1 is also thermolabile and is sta

104 iphosphatase (CaCet1p), a 449-amino acid RNA guanylyltransferase (Cgt1p), and a 474-amino acid RNA (g

105 g bacteria, depends on a bifunctional kinase/guanylyltransferase (CobP) enzyme to convert adenosylcob

106 binamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) from Salmonella typhimurium b

107 binamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) from Salmonella typhimurium h

108 e three-dimensional crystal structure of the guanylyltransferase (CobY) enzyme from the archaeon Meth

109 , we report the crystal structure of the RNA guanylyltransferase component of mammalian capping enzym

110 targeted to pre-mRNAs through binding of the guanylyltransferase component of the capping apparatus t

111 1p homodimerization and Cet1p binding to the guanylyltransferase component of the capping apparatus.

112 iphosphatase and its physical linkage to the guanylyltransferase component of the capping apparatus.

113 us consisting of separate triphosphatase and guanylyltransferase components, which we characterize bi

114 This implies an allosteric change in guanylyltransferase conformation that is specified by th

115 carboxy-terminal half of the C. fasciculata guanylyltransferase containing the six signature motifs

116 Deletion analysis of the Candida guanylyltransferase demarcates an N-terminal domain, Cgt

117 n, Cgt1(1-367)p, suffices for binding to the guanylyltransferase docking site on yeast RNA triphospha

118 ely, mutation of the invariant lysine in the guanylyltransferase domain abolished the guanylyltransfe

119 pping enzyme consisting of an amino-terminal guanylyltransferase domain and a carboxyl-terminal methy

120 nonoverlapping functional domains; (ii) the guanylyltransferase domain Mce1(211-597) is catalyticall

121 Yet, fusion of Cth1p in cis to the guanylyltransferase domain of mammalian capping enzyme a

122 These results demonstrate that the guanylyltransferase domain of mammalian capping enzyme s

123 ed by fusion of D1(1-545)p to the C-terminal guanylyltransferase domain of mammalian capping enzyme,

124 fusion of the mutated triphosphatases to the guanylyltransferase domain of mammalian capping enzyme.

125 re we conducted a mutational analysis of the guanylyltransferase domain of the mouse capping enzyme M

126 49)p is completely restored by fusion to the guanylyltransferase domain of the mouse capping enzyme.

127 dogenous guanylyltransferase Ceg1; (iii) the guanylyltransferase domain per se binds to the phosphory

128 The guanylyltransferase domain was necessary and sufficient

129 id nematode protein consists of a C-terminal guanylyltransferase domain, which is homologous to Ceg1

130 riphosphatase component of LEF-4 but not the guanylyltransferase domain.

131 NTP:AdoCbi kinase (EC 2.7.7.62)/GTP:AdoCbi-P guanylyltransferase (EC 3.1.3.73) and is required for de

132 sisting of separate triphosphatase (EcCet1), guanylyltransferase (EcCeg1), and methyltransferase (Ecm

133 ve tunnel-type triphosphatase and a separate guanylyltransferase encoded by the red alga Cyanidioschy

134 mide kinase/GTP:adenosylcobinamide-phosphate guanylyltransferase enzyme (CobU in Salmonella enterica)

135 hoamide linkage, a characteristic feature of guanylyltransferase enzyme.

136 d physical linkage of the triphosphatase and guanylyltransferase enzymes that catalyze cap formation.

137 al linkage of the RNA triphosphatase and RNA guanylyltransferase enzymes that catalyze mRNA cap forma

138 eparately encoded RNA triphosphatase and RNA guanylyltransferase enzymes.

139 Guanylyltransferase experiments, however, showed that fo

140 7-aa mouse capping enzyme and the C-terminal guanylyltransferase fragment (residues 211-597), unlike

141 s hSPT5 did not increase the activity of the guanylyltransferase fragment.

142 The smaller size of RNA capping guanylyltransferases from other organisms suggested that

143 t functional conservation of eukaryotic mRNA guanylyltransferases from yeast to mammals and indicate

144 inhibition of capping enzyme GTP binding and guanylyltransferase function.

145 NS5 has 5'-RNA methyltransferase (MT)/guanylyltransferase (GT) activities within the N-termina

146 arrying both RNA triphosphatase (RTPase) and guanylyltransferase (GTase) activities.

147 Interactions between RNA guanylyltransferase (GTase) and the C-terminal domain (C

148 ne (m7G) cap structure is known to require a guanylyltransferase (GTase) as well as a 5' triphosphata

149 ds, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein

150 The enzyme guanylyltransferase (GTase) plays a central role in the

151 the 5' end of the viral genome and possesses guanylyltransferase, guanine-N7-methyltransferase, and n

152 stood but presumably require triphosphatase, guanylyltransferase, [guanine-N-7]- and [ribose-2'-O]-me

153 Although numerous guanylyltransferases have been identified, studies which

154 ; VP2, the core capsid protein; and VP3, the guanylyltransferase, have affinity for RNA but that only

155 hey express either the S. pombe or mammalian guanylyltransferase in lieu of Ceg1.

156 Stimulation of guanylyltransferase increases with the number of Ser-5-p

157 RNA guanylyltransferase is an essential enzyme that catalyze

158 though no interaction between Cet1 and mouse guanylyltransferase is detectable, both proteins are pre

159 Therefore, the yeast mRNA guanylyltransferase is regulated by allosteric interacti

160 ctose is catalyzed by the octose 1-phosphate guanylyltransferase, LmbO.

161 consisting of a bifunctional triphosphatase-guanylyltransferase Mce1p and the methyltransferase Hcm1

162 TbCgm1 (T. brucei cap guanylyltransferase-methyltransferase) is a novel bifunc

163 s further modified through the activity of a guanylyltransferase, MobA, which converts MoCo to bis-mo

164 binamide kinase/adenosylcobinamide-phosphate guanylyltransferase needed to convert cobinamide to aden

165 reaction indicating that the protein is the guanylyltransferase of the virus.

166 el active-site motif is proposed for the RNA guanylyltransferases of mammalian reoviruses and other R

167 Decreasing either guanylyltransferase or methyltransferase resulted in cas

168 The mRNA guanylyltransferase, or mRNA capping enzyme, cotranscrip

169 A 116-amino acid fragment of the guanylyltransferase Pce1 suffices for binding to the Spt

170 yeast whereby the triphosphatase (Pct1) and guanylyltransferase (Pce1) enzymes of the capping appara

171 e we show that the triphosphatase (Pct1) and guanylyltransferase (Pce1) enzymes of the fission yeast

172 show that P. falciparum encodes separate RNA guanylyltransferase (Pgt1) and RNA triphosphatase (Prt1)

173 gi and Chlorella virus encode monofunctional guanylyltransferase polypeptides that lack triphosphatas

174 ures of Chlorella virus and Candida albicans guanylyltransferases, provide a coherent picture of the

175 The guanylyltransferase reaction has been proposed to procee

176 lambda2 mediates the guanylyltransferase reaction in cap formation and was pr

177 tion of the GTP:adenosylcobinamide-phosphate guanylyltransferase reaction shows the covalent CobU-GMP

178 e ORF YGR024c (THG1) is responsible for this guanylyltransferase reaction.

179 omain which catalyzes the triphosphatase and guanylyltransferase reactions.

180 strains missing either RNA triphosphatase or guanylyltransferase required terminal sequences not pres

181 purified from Escherichia coli catalyzes the guanylyltransferase step of G(-1) addition using a ppp-t

182 g DNA viruses in that the triphosphatase and guanylyltransferase steps of cap formation are catalyzed

183 localize the guanylyltransferase-binding and guanylyltransferase-stimulation functions of Cet1p to a

184 g enzyme Mce1 (a bifunctional triphosphatase-guanylyltransferase) substitutes for Cet1 in vivo.

185 Although the guanylyltransferase subunit can bind alone to the CTD, i

186 03R is more closely related to the yeast RNA guanylyltransferases than it is to the multifunctional c

187 tional studies of the bifunctional bacterial guanylyltransferase that catalyze both ATP-dependent cor

188 osphate of triphosphate-terminated RNA and a guanylyltransferase that reacts with GTP to form a coval

189 To demonstrate that LEF-4 was the guanylyltransferase, the single subunit was overexpresse

190 tRNA(His) guanylyltransferase (Thg1) adds a single guanine to the

191 e activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no examples of eu

192 The tRNA(His) guanylyltransferase (Thg1) family comprises a set of uni

193 The tRNA(His) guanylyltransferase (Thg1) is a member of a unique enzym

194 The yeast tRNA(His) guanylyltransferase (Thg1) is an essential enzyme in yea

195 tRNA(His) guanylyltransferase (Thg1) post-transcriptionally adds a

196 tRNA(His) guanylyltransferase (Thg1) specifically adds the guanyly

197 and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well as Thg1-like protein

198 addition reaction catalyzed by the tRNA(His) guanylyltransferase (Thg1).

199 Yeast tRNA(His) guanylyltransferase, Thg1, is an essential protein that

200 essential Saccharomyces cerevisiae tRNA(His) guanylyltransferase (Thg1p) is responsible for the unusu

201 inamide ring and subsequently functions as a guanylyltransferase to form adenosylcobinamide.GDP.

202 tiating ribonucleotide triphosphate, we used guanylyltransferase to in vitro cap the replication inte

203 us D1(1-545)p, an RNA triphosphatase and RNA guanylyltransferase-to function in the budding yeast Sac

204 mechanism for mRNA cap formation in that the guanylyltransferase transfers GDP rather than GMP onto t

205 RNA guanylyltransferases typically have a KxDG motif in whic

206 RNA (dsRNA) genome, RNA polymerase VP1, and guanylyltransferase VP3 and are enclosed within a lattic

207 corporation of the polymerase enzyme VP1 and guanylyltransferase VP3 into the core of the virion.

208 Delta cells, thus proving that (i) the viral guanylyltransferase was active in vivo and (ii) the mamm

209 ain of Saccharomyces cerevisiae lacking mRNA guanylyltransferase was complemented for growth by the m

210 with mutations in CEG1, encoding the nuclear guanylyltransferase, were also synthetic lethal with xrn

211 adenosylcobinamide kinase/adenosylcobinamide guanylyltransferase where a P-loop is located at the end

212 PBCV-1 encodes its own mRNA guanylyltransferase, which catalyzes the addition of GMP

213 nsable when Ceg1 is substituted by the mouse guanylyltransferase, which does not require allosteric a

214 :adenosylcobinamide-phosphate [GTP:AdoCbi-P] guanylyltransferase) whose AdoCbi kinase activity is nec

215 D1 subunit that specifically inactivate the guanylyltransferase without affecting the triphosphatase

216 anisms suggested that the lambda2-associated guanylyltransferase would be only a part of this protein