ライフサイエンスコーパス: guide RNA

1 efficient genome editing in the presence of guide RNA.

2 es, insertions and deletions relative to the guide RNA.

3 m crystal structure of human Argonaute4 with guide RNA.

4 that precede the pairing of target DNA with guide RNA.

5 tivity but that this activity depends on the guide RNA.

6 arget loci by competition with the on-target guide RNA.

7 lation of RNA with specificity determined by guide RNAs.

8 tivity with the optimal 22-nucleotide length guide RNAs.

9 d A-to-I editing through the use of tailored guide RNAs.

10 ls, even when using single 20- to 27-nt-long guide RNAs.

11 complexes to target loci by modified single guide RNAs.

12 Our study sheds light on the role of tiny guide RNAs.

13 ng-optimized libraries of hybrid Cas9-Cas12a guide RNAs.

14 g on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs).

15 iated virus 9) to deliver multiplexed single guide RNAs against Ube2v1 in cardiac-specific Cas9 mice

16 cid interactions, and we show that caging of guide RNA allows for tunable and reversible control over

17 Targeting of dCas9 by a mismatch-containing guide RNA also increases CAN1 mutation frequency, partic

18 with BE3 or HF1-BE3 in the absence of single-guide RNA also results in the rise of genome-wide SNVs.

19 RISPRi transgene broadly expressing a single guide RNA and a catalytically dead Cas9 fused to the KRA

20 ic recognition of the amplicon by the CRISPR guide RNA and Cas12a enzyme improved specificity.

21 techniques, including mRNA, Cas9 mRNA/single guide RNA and Cas9 ribonucleoprotein complexes, and is e

22 omain (AceCas9-DeltaHNH) bound with a single guide RNA and DNA substrates, one with the correct and t

23 asX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an e

24 in 9 (Cas9) complexed with a specific single-guide RNA and immobilized on the transistor to yield a l

25 ucing the formation of an R-loop between the guide RNA and its genomic target.

26 stal structure of CdCas9 in complex with the guide RNA and its target DNA at 2.9 angstrom resolution.

27 en by complementary base pairing between the guide RNA and target DNA, Cas9-DNA interactions, and ass

28 e sites and end trimming varied by nuclease, guide RNA and the positions of mispaired nucleotides.

29 L) system to two genomic anchors with CRISPR guide RNAs and induce their spatial colocalization via l

30 ased on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by assay of transpos

31 achieved by electroporation of small single-guide RNAs and ssODN repair templates alone.

32 omic sites together with moderately modified guide RNA, and show its therapeutic potential in correct

33 a com aptamer preserves the functions of the guide RNA, and the com-modified sgRNA can package Cas9/s

34 rary that consisted of around 123,000 single-guide RNAs, and profiled genes whose loss in tumour cell

35 loop structure may explain the uniformity of guide RNA architecture and the single-active-site cleava

36 Since CRISPR guide RNAs are longer than transcription factor binding

37 Guide RNAs are privatized to be recognized only by the t

38 mbly, expression and processing of synthetic guide RNA arrays in vivo.

39 ntially easier to construct than Cas9 single-guide RNA arrays, facilitating multiplex genome engineer

40 Here we describe CRISPR Guide RNA Assisted Reduction of Damage (CRISPR GUARD) as

41 imized insertion of transposable elements by guide RNA-assisted targeting (INTEGRATE) system achieves

42 562 cells using the Cas9 nuclease and paired guide RNAs at high efficiencies, followed by high-throug

43 A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was develo

44 ogenitor cells through the use of Bcor small guide RNAs (Bcor sgRNAs).

45 identified membranous protein that directly guides RNA-binding protein into EVs.

46 structure of AcrIIA4 in complex with single-guide RNA-bound SpyCas9, thereby establishing that AcrII

47 Therefore, chemical modification of the guide RNA can be used to characterize structure-activity

48 However, we discover that short guide RNAs can also support base editing if they contain

49 A short RNA string (the CRISPR guide RNA) can guide the Cas9 endonuclease to specific l

50 dal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phen

51 Cas9 nucleases complexed with a guide RNA (Cas9-gRNA) find their targets by scanning and

52 , we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotein complexes into fertiliz

53 e Sleeping Beauty (SB) transposon and single guide RNA cassette are nested in an adeno-associated vir

54 B-mediated genomic integration of the single guide RNA cassette enables efficient gene editing in pri

55 Tn7-CRISPR-Cas elements evolved a system of guide RNA categorization to accomplish the same two-path

56 9 targeted to the CDKL5 promoter using three guide RNAs causes significant reactivation of the inacti

57 e locations of Cas9-guided cleavage for four guide RNAs, characterize associated deletions, and show

58 These RNAs, named cleavage-inducing tiny guide RNAs (cityRNAs), conversely lower the activity of

59 ome eggs were exposed to Cas9 complexed with guide RNA complementary to omega1 by electroporation or

60 e, which is targeted by a linked Cas protein-guide RNA complex(3,4).

61 do not prevent cleavage in vitro by the Cas9/guide RNA complex.

62 onic lipid-mediated in vivo delivery of Cas9-guide RNA complexes can ameliorate hearing loss in a mou

63 weight DNA targets with custom-designed Cas9-guide RNA complexes followed by sequencing with barcoded

64 ssociated protein 9) DNA nuclease and single guide RNA components, and differences in the relative ed

65 associated 9 from Staphylococcus aureus) and guide RNA constructs into an adeno-associated virus vect

66 ome-integrated NOT/NOR gates based on single guide RNAs (CRISPR-dCas9) to inform a Bt user constraint

67 i-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destru

68 Furthermore, using a single guide RNA, dCas9-SunTag-DNMT3A is able to methylate a 4.

69 After activation by guide RNA-defined inputs, Cas12a cleaves DNA in the gels

70 nactivated CRISPR-Cas variants might mediate guide RNA-dependent integration of the respective transp

71 e an open-source software tool (FLASHit) for guide RNA design.

72 mismatch with implications for mechanism and guide-RNA design.

73 We demonstrate that a dual-guide RNA (dgRNA) with a modified tracrRNA can improve r

74 ng off-targets sites by co-delivery of short guide RNAs directed against off-target loci by competiti

75 leotide small interfering RNAs (siRNAs) that guide RNA-directed DNA methylation.

76 ve been coopted multiple times in nature for guide RNA-directed transposition by Tn7-like elements.

77 forming a 20-nucleotide R-loop in which the guide RNA displaces one strand of a double-helical DNA s

78 ive network of contacts between REC3 and the guide RNA-DNA heteroduplex.

79 Multiplexing of several guide RNAs does not increase the efficiency of methylati

80 These results suggest that after guide RNA-driven conformational changes, water-mediated

81 These activatable guide RNAs enable temporal and post-transcriptional cont

82 An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromos

83 ce in eukaryotes, where they function as RNA-guided RNA endonucleases.

84 odifications of adenine base editor mRNA and guide RNA expand the applicability of CRISPR-associated

85 e a cardiac phenotype, irrespective of short guide RNA exposure or the level of Cas9 expression.

86 turbations because they can process multiple guide RNAs expressed as a single transcript, and subsequ

87 Finally, multiple guide RNA expression allows simultaneous inhibition of m

88 Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utili

89 Binding of guide RNA fastens the subdomains, thereby rearranging th

90 iological applications such as the design of guide RNA for CRISPR experiments.

91 e most efficient and less off-target effects guide RNAs for a given gene.

92 study will assist in the rational design of guide RNAs for ADAR-mediated RNA base editing.

93 We describe pgFARM (paired guide RNAs for alternative exon removal), a CRISPR-Cas9-

94 short hairpin RNAs for RNAi experiments and guide RNAs for CRISPR-mediated genome editing.

95 mation reveals promising avenues to engineer guide RNAs for enhanced CRISPR-Cas functionality for gen

96 ems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mob

97 tiple mechanisms allow functionally distinct guide RNAs for transposition: a conventional system capa

98 ments, including resources to design optimal guide RNAs for various modes of manipulation and to anal

99 DISCOVER-Seq works with multiple guide RNA formats and types of Cas enzymes, allowing cha

100 lity to join to specific genomic Cas9/single-guide RNA-generated bait DSBs.

101 cores for all guides, thereby offering rapid guide RNA generation and selection.

102 d mitochondrial genes and minicircle-encoded guide RNA genes is essential to maintain efficient respi

103 vered in a lentiviral vector with one CRISPR guide RNA (gRNA) achieved potent and specific PTEN repre

104 can tolerate up to seven mismatches between guide RNA (gRNA) and target DNA.

105 effects of combinatorial mismatches between guide RNA (gRNA) and target nucleotides, both in the see

106 RISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations

107 rred allele is selectively targeted for Cas9/guide RNA (gRNA) cleavage, and a more general approach,

108 Truncation of guide RNA (gRNA) from the 5' end enables the application

109 l library of NOR gates that directly convert guide RNA (gRNA) inputs into gRNA outputs, enabling the

110 CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the g

111 elop a method for rapid generation of custom guide RNA (gRNA) libraries using arrayed single-stranded

112 ins on a single polycistronic vector and the guide RNA (gRNA) on a separate plasmid.

113 ess the protospacer adjacent motif (PAM) and guide RNA (gRNA) requirements of 79 Cas9 proteins, thus

114 s improvements, such as modifications to the guide RNA (gRNA) scaffold and the development of gRNA on

115 wn as Cas12a) and Cas9, exhibit differential guide RNA (gRNA) sequence requirements for cleavage of t

116 em widely vary because of the differences in guide RNA (gRNA) sequences and genomic environments.

117 he CRISPR-Cpf1 system varies among different guide RNA (gRNA) sequences.

118 rotein 9 nuclease (Cas9) system depends on a guide RNA (gRNA) to specify its target.

119 njection of CRISPR components (Cas9 mRNA and guide RNA (gRNA)) into the oviducts of pregnant females

120 ession of the dead Cas9 (dCas9) effector and guide RNA (gRNA), which can vary substantially depending

121 ISPR-Cas12 enzyme complexes with a synthetic guide RNA (gRNA).

122 contain genetic clones harboring individual guide RNAs (gRNA), we identify RNA-binding proteins (RBP

123 eus, and Francisella novicida complexed with guide RNAs (gRNAs) (SpCas9-gRNA, SaCas9-gRNA, and FnCas9

124 ion lymphoma (PEL) cells by coexpressing two guide RNAs (gRNAs) and Cas9 from a single expression vec

125 as9 technology to simultaneously express two guide RNAs (gRNAs) and Cas9 from a single expression vec

126 xplore a homing system architecture in which guide RNAs (gRNAs) are multiplexed, increasing the effec

127 However, few published guide RNAs (gRNAs) are predicted to cleave the majority

128 and acts in conjunction with unconventional guide RNAs (gRNAs) designed to induce loops at the targe

129 ovide guidelines for the synthesis of Cas12a guide RNAs (gRNAs) for in vitro applications.

130 (rRNAs) and proteins and minicircles bearing guide RNAs (gRNAs) for mRNA editing.

131 ibe a CRISPR-based system that uses pairs of guide RNAs (gRNAs) to program thousands of kilobase-scal

132 The key components of CRISPR/Cas9 are guide RNAs (gRNAs) which determine specific sequence tar

133 els by targeting a haplolethal gene with two guide RNAs (gRNAs) while also providing a rescue allele.

134 by hundreds of species of minicircle-encoded guide RNAs (gRNAs), but the precise number of minicircle

135 To define rules for the design of Cas13d guide RNAs (gRNAs), we conducted massively parallel scre

136 One, germline-expressed Cas9 and guide RNAs (gRNAs)-the Cleaver-cleaves and thereby disru

137 bryos using photochemically activated, caged guide RNAs (gRNAs).

138 iated viral (AAV) vectors expressing Cas9 or guide RNAs (gRNAs).

139 specified by easily assembled gene-specific guide-RNA (GS-gRNA) vectors.

140 Cas9 protein (dCas9) and programmable single guide RNAs, has emerged as a powerful genetic tool to di

141 A homing guide RNA (hgRNA) scaffold directs the Cas9-hgRNA comple

142 y structure onto the spacer region of single guide RNAs (hp-sgRNAs) can increase specificity by sever

143 CRaft-ID; CRISPR-based microRaft followed by guide RNA identification).

144 Mapping top functional guide RNAs identified key protein interfaces where in-fr

145 Here, we present GRIBCG (Guide RNA Identifier for Balancer Chromosome Generation)

146 By expressing two guide RNAs in tandem to simultaneously knock down kinesi

147 etions, and will facilitate design of SpCas9 guide RNAs in therapeutically important primary human ce

148 that they can also induce transcriptome-wide guide-RNA-independent editing of RNA bases(5), and creat

149 that enables different sets of user-defined guide RNA inputs to program a single transcriptional reg

150 ts were observed but only with a promiscuous guide RNA intentionally designed to validate our approac

151 y challenges associated with the delivery of guide RNA into the mitochondria(4).

152 The CRISPR guide RNA is essential for gene editing systems.

153 Controlled by recombination, a single guide RNA is stochastically chosen from a set targeting

154 d virus serotype 9 to deliver a single short guide RNA is target dependent.

155 ts that these anti-CRISPRs manipulate single guide RNA length, loading or stability.

156 However, guide RNA libraries are costly to synthesize, and their

157 mputationally expensive for designing CRISPR guide RNA libraries from large genomes.

158 MultiGuideScan makes it possible to design guide RNA libraries from large genomes.

159 GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and

160 Using pooled lentiviral single-guide RNA libraries, we conducted a genome-wide loss-of-

161 eScan software is developed to design CRISPR guide RNA libraries, which can be used for genome editin

162 cale screening by combining Cas9 with pooled guide RNA libraries.

163 and facilitates the generation of optimized guide RNAs libraries.

164 MultiGuideScan speeds up the guide RNA library designing about 9-12 times on a 32-pro

165 Here, we present an efficient guide RNA library designing tool (MultiGuideScan) by imp

166 g using a ubiquitin regulator-focused single-guide RNA library in HL lines carrying either wild-type

167 Facile single-guide RNA library synthesis allows CRISPR-Cas screening

168 igment epithelial cells using a focused dual guide RNA library targeting 852 DDR-associated genes.

169 tly analyzes screens performed with the same guide RNA library.

170 Injection of Cas9-guide RNA-lipid complexes targeting the Tmc1(Bth) allele

171 s double-stranded DNA sequences specified by guide RNA molecules and flanked by a protospacer adjacen

172 te Expression) for quantifying the impact of guide RNAs on a target gene's expression in a pooled, so

173 ural networks (CNN) approach to predict Cpf1 guide RNAs on-target activity and off-target effects usi

174 iciency of CRISPR/Cas9-mediated editing with guide RNAs only 1-10 bp apart.

175 ere we describe two self-copying (or active) guide RNA-only genetic elements, called e-CHACRs and ERA

176 Targeting of MAP4K4 with single guide RNAs or a MAP4K4 inhibitor reduced migration and i

177 transferred to other systems with different guide RNAs or Cas9 ortholog proteins.

178 anscriptase, programmed with a prime editing guide RNA (pegRNA) that both specifies the target site a

179 iptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells

180 The design of prime-editing guide RNAs (pegRNAs), which must be customized for each

181 ond CRISPR library was made containing three guide RNAs per construct to target 18 putative transport

182 ion using electroporation of Cas9 and single guide RNA plasmids.

183 d tRNA scaffold that enables highly specific guide RNA production from a Pol-II promoter.

184 a double-CRISPR/Cas9 strategy, in which two guide RNAs promote replacement of a candidate gene with

185 t HLA-DRB, -DQB1, and -DPB1 through a single guide RNA recognizing a conserved region in exon 2.

186 eins are guided to specific targets by small guide RNAs, referred to as piRNAs or 21U RNAs in Caenorh

187 on to an optimized version of the Csy4-based guide RNA release system.

188 th terminal C/D and internal C'/D' motifs of guide RNAs, respectively.

189 ding of Cas9 plasmid as well as Cas9 protein/guide RNA ribonucleoprotein complex (RNP), while liposom

190 c acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and susp

191 ISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical inte

192 transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene

193 repeatedly at the same off-target sites in a guide-RNA-sequence-dependent manner, driven by the mecha

194 There exist a number of tools to design the guide RNA sequences and predict potential off-target sit

195 ion importance analysis, the key features of guide RNA sequences are identified, which determine the

196 a mechanism by which both the substrate and guide RNA sequences determine the degree of methylation.

197 e additionally report numerous highly active guide RNA sequences sharing minimal homology that may en

198 ctive Cas9 (dCas9) and by providing a single guide RNA (sgRNA) against the human Alu retrotransposon.

199 ith DNA when mismatches to the target single-guide RNA (sgRNA) are present in the sgRNA:DNA heterodup

200 g the adenine base editor (ABE) and a single-guide RNA (sgRNA) can correct an A>G splice-site mutatio

201 can be shielded from the active Cas9*single guide RNA (sgRNA) complex through the co-administration

202 nt CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) delivery is based on the premise that

203 Existing single-guide RNA (sgRNA) design tools mainly depend on a sgRNA

204 lizes a ribozyme-guide-ribozyme (RGR) single guide RNA (sgRNA) expression strategy with RNA polymeras

205 ined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers re

206 henotype imaging with high-throughput single guide RNA (sgRNA) identification in individual cells.

207 ity evaluation of 100nt long, used as single guide RNA (sgRNA) in CRISPR technology, and promoted as

208 tagged Cas9 and lentivirus encoding a single guide RNA (sgRNA) in primary human lung microvascular EC

209 is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the specific cell and organ.

210 ion of a mixture of Cas9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes.

211 a web tool for identifying CRISPR-Cas single guide RNA (sgRNA) targets.

212 r the delivery of Cas9 nuclease and a single-guide RNA (sgRNA) that enables the controlled stoichiome

213 Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacon

214 yogenes (SpCas9), with a complementary small guide RNA (sgRNA) to inactivate endogenous genes resulti

215 y sequences to constitutively express single-guide RNA (sgRNA) transcripts.

216 element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the D

217 (SBH) structure at the 5' end of the single guide RNA (sgRNA), which abrogates the function of CRISP

218 g efficacy is mainly dependent on the single guide RNA (sgRNA), which guides Cas9 for genome cleavage

219 utation to -124C was achieved using a single guide RNA (sgRNA)-guided and catalytically impaired Camp

220 ABE8s induce no significant levels of single guide RNA (sgRNA)-independent off-target adenine deamina

221 basic module consists of an inactive single-guide RNA (sgRNA)-like component that is converted to an

222 le cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released after CR

223 y simply changing the sequence of the single guide RNA (sgRNA).

224 al muscle myoblast cell lines using a single-guide RNA (sgRNA).

225 a purified protein in complex with a single guide RNA (sgRNA).

226 Using single-guide RNA (sgRNA)/dCas9 and small interfering RNA (siRNA

227 loxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotide

228 e dCas9 that recognizes two different single guide RNAs (sgRNA).

229 guanine nucleotides at the 5' ends of single guide RNAs (sgRNAs) account for diminished CRISPR-Cas9 a

230 Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large ge

231 ination (HR), we designed a series of single guide RNAs (sgRNAs) flanking the mutation and provided d

232 n addition, infected cells expressing single guide RNAs (sgRNAs) for both of these genes displayed lo

233 ess to a database of over 3.4 million single guide RNAs (sgRNAs) for iSTOP (sgSTOPs) targeting 97%-99

234 GRIBCG identifies single guide RNAs (sgRNAs) for use with Streptococcus pyogenes

235 PR)-based knockout by analysis of 373 single guide RNAs (sgRNAs) in 6 cells lines and show that the o

236 To address this challenge, we design single-guide RNAs (sgRNAs) integrated with up to 16 MS2 binding

237 eting SURF4 with multiple independent single guide RNAs (sgRNAs) resulted in intracellular accumulati

238 In MAD-DASH, Cas9 is complexed with single guide RNAs (sgRNAs) targeting adapter dimer ligation pro

239 F loop anchors in K562 cells were not single guide RNAs (sgRNAs) that disrupted gene expression near

240 say by showing that the expression of single guide RNAs (sgRNAs) that target genes encoding known thr

241 CRISPR/Cas9 system requires short guide RNAs (sgRNAs) to direct genome modification.

242 RISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic instability and phe

243 Three individual single guide RNAs (sgRNAs) were designed per gene to facilitate

244 ing CRISPR interference and series of single-guide RNAs (sgRNAs) with systematically modulated activi

245 Compared with single guide RNAs (sgRNAs), pegRNAs have an additional 3' exten

246 SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sit

247 igh quantities of biologically active single guide RNAs (sgRNAs).

248 d by reliance on indirect indexing of single-guide RNAs (sgRNAs).

249 Guide RNAs shorter than 16 nucleotides in length effecti

250 While our dCas9 circuits using 103-nt guide RNAs showed irregular fluctuations with a wide dis

251 eering aimed at altering catalytic function, guide RNA specificity, and PAM requirements and reducing

252 Guide RNA strands for directed DNA editing by ADAR were

253 dCas9-induced mutations cluster near the guide RNA target region and are comprised of single nucl

254 e of the most prevalent polymorphisms in the guide RNA target site in collections of colonized and wi

255 lex mutations, depending upon the particular guide RNA target.

256 a lentivirus expressing an ABE and a single-guide RNA targeting a de novo nonsense mutation in the R

257 ors encoding nuclease-dead Cas9 and a single-guide RNA targeting CUG repeats results in the expressio

258 All-in-one AAV delivery of Nme2Cas9 with a guide RNA targeting Pcsk9 in adult mouse liver produces

259 protein levels of CEBPE in cells expressing guide RNA targeting the +6-kb region.

260 (PigGeCKO) library containing 85,674 single guide RNAs targeting 17,743 protein-coding genes, 11,053

261 As a proof-of-concept, we delivered short guide RNAs targeting 3 genes critical for cardiac physio

262 ovirus to deliver the nickase Cas9(D10A) and guide RNAs targeting the breakpoint sequences, and anoth

263 We designed guide-RNAs targeting three loci (POLLED, H11, and ZFX) i

264 ryotic RNA interference, which also uses RNA-guided RNA targeting to silence actively transcribed gen

265 bled with a CRISPR RNA (crRNA) forms a crRNA-guided RNA-targeting effector complex.

266 The RNA-guided, RNA-targeting clustered regularly interspaced sh

267 degrade invasive genetic elements by an RNA-guided, RNA-targeting multisubunit interference complex.

268 We applied CHANGE-seq to 110 single guide RNA targets across 13 therapeutically relevant loc

269 editing than the wild-type CBE and, for most guide RNAs tested, no substantial reduction in editing e

270 A short Cas9 variant and guide RNA that target about 75 Snord115 genes were packa

271 target editing can be minimized by designing guide RNAs that are different from other genomic locatio

272 ministration of dead-RNAs (dRNAs), truncated guide RNAs that direct Cas9 binding but not cleavage.

273 Spacers are transcribed into guide RNAs that direct the Cas9 nuclease to its target o

274 as9 (dCas9), VP64 transactivators and single-guide RNAs that target the Lama1 promoter.

275 limited by signal variability from different guide RNAs that target the same gene, which confounds ge

276 ntified a pair of Staphylococcus aureus Cas9 guide RNAs that were highly active and specific to the h

277 hese nucleases readily utilized each other's guide RNAs, they exhibited distinct PAM profiles and app

278 analog found in prokaryotes, allows a single-guide RNA to direct a CRISPR-associated protein (Cas) wi

279 bioorthogonal click chemistry for remodeling guide RNA to display synthetic molecules on target genes

280 able genome engineering (CREATE), links each guide RNA to homologous repair cassettes that both edit

281 get-recognition sequence of the Cas12a-bound guide RNA to irreversibly inactivate the Cas12a complex.

282 destroy bacteriophages and plasmids, using a guide RNA to locate complementary RNA molecules from the

283 ng a 20-nucleotide section of its associated guide RNA to one DNA strand, forming an R-loop structure

284 Type III CRISPR-Cas systems employ guide RNA to recognize complementary RNA targets, which

285 ffector Cas nucleases can be programmed with guide RNAs to access desirable genomic sites.

286 highly complex RNA editing system that uses guide RNAs to direct the insertion and deletion of uridi

287 is process is the in silico design of single guide RNAs to efficiently and specifically target a site

288 delivering reagents such as Cas9 and single guide RNAs to explants in culture.

289 : a conventional system capable of acquiring guide RNAs to new plasmid and phage targets and a second

290 e systems have been naturally adapted to use guide RNAs to specifically direct transposition into the

291 isiae revealed that the Exo9 central channel guides RNA to either Rrp6 or Rrp44 using partially overl

292 We identified an efficient OCT4-targeting guide RNA using an inducible human embryonic stem cell-b

293 sites targeted by imperfectly matched single guide RNAs was observed, suggesting that while the prima

294 nd the folding stability of the whole single guide RNA, we devised a unified, physical model that can

295 mportant mismatches at the distal end of the guide RNA, we performed kinetic analyses on the high-fid

296 vidence of off-target cleavage activity when guide RNAs were bioinformatically predicted to be specif

297 use an active genetic element that encodes a guide RNA, which is embedded in the mouse tyrosinase (Ty

298 We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in th

299 e sampling complexity from 1012 to 3 million guide RNAs with only a small loss in accuracy (R2R2 ~ 0.

300 experiment design, allowing the selection of guide RNAs with predicted repair outcome signatures enri