ライフサイエンスコーパス: haemolytica

1 the leukotoxin operon in ovine strains of M. haemolytica.

2 ng the different evolutionary lineages of M. haemolytica.

3 ons, to study transcription initiation in M. haemolytica.

4 ast, the lktD gene is highly conserved in M. haemolytica.

5 after respiratory infection with Pasteurella haemolytica.

6 s somnus, Neisseria species, and Pasteurella haemolytica.

7 ) and CD18(-) cattle after inoculation of P. haemolytica.

8 antibody titers against RBCV and Pasteurella haemolytica.

9 t PlpE contribute to host defense against P. haemolytica.

10 elium after acute infection with Pasteurella haemolytica.

11 [32K and 35K, respectively]) of Pasteurella haemolytica.

12 eria meningitidis, and LpsA from Pasteurella haemolytica.

13 ges and PMNs of BHS and DS in response to M. haemolytica.

14 y the bovine respiratory pathogen Mannheimia haemolytica.

15 a function and 436 of which are unique to M. haemolytica.

16 kotoxin secreted by Mannheimia (Pasteurella) haemolytica.

17 st the R2 region are effective in killing M. haemolytica.

18 contributed to strain diversification in M. haemolytica.

19 5%), while fewer calves were positive for M. haemolytica (13%).

20 investigated in 31 Mannheimia (Pasteurella) haemolytica, 6 Mannheimia glucosida, and 4 Pasteurella t

21 nd lktD genes in 23 Mannheimia (Pasteurella) haemolytica, 6 Mannheimia glucosida, and 4 Pasteurella t

22 Mannheimia haemolytica, a commensal organism of the upper respirato

23 A Pasteurella haemolytica A1 gene was identified from a recombinant li

24 Mannheimia (Pasteurella) haemolytica A1 produces several virulence factors that p

25 mal CD18 expression) were inoculated with P. haemolytica A1 via a fiberoptic bronchoscope and euthani

26 The draft genome sequence of Mannheimia haemolytica A1, the causative agent of bovine respirator

27 otective antigen for GBM, E. coli K1, and P. haemolytica A2, protein conjugates of it are easy to pre

28 (GBMs), Escherichia coli K1, and Pasteurella haemolytica A2.

29 the family Pasteurellaceae indicates that M. haemolytica, Actinobacillus pleuropneumoniae, and Haemop

30 It is proposed that the OmpA protein of M. haemolytica acts as a ligand and is involved in binding

31 In this study, we demonstrated that M. haemolytica adhered to bovine bronchial epithelial cells

32 ndicating that PlpE is surface exposed in P. haemolytica and assumes a similar surface-exposed confor

33 d be useful for future genetic studies in P. haemolytica and could potentially be applied to other me

34 of all known Hsd aa sequences placed the P. haemolytica and H. influenzae proteins into a new group

35 n vectors that replicate both in Pasteurella haemolytica and in Escherichia coli were constructed bas

36 he important respiratory pathogen Mannheimia haemolytica and its leukotoxin (LKT).

37 nderstanding NET formation in response to M. haemolytica and its LKT provides a new perspective on ho

38 The OmpA proteins of M. haemolytica and M. glucosida contain four hypervariable

39 class III alleles were present in certain M. haemolytica and M. glucosida isolates.

40 A genes are highly diverged from those of M. haemolytica and M. glucosida, and evidence is presented

41 sses, classes I to IV, were identified in M. haemolytica and M. glucosida.

42 (zinc saline solution) induced killing of P. haemolytica and other bacteria comparable to defensins a

43 ed to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynami

44 We found that intact P. haemolytica and recombinant E. coli expressing PlpE are

45 culated with serotype A1 or A2 strains of M. haemolytica and the course of infection followed over a

46 our matched pairs of isolates of Pasteurella haemolytica and three matched pairs of isolates of Paste

47 uantitate leukotoxin promoter activity in P. haemolytica and to demonstrate that transcription was ma

48 od plasma from dairy calves infected with M. haemolytica and validates the spectral biochemistry usin

49 ociated with bovine M. haemolytica, ovine M. haemolytica, and M. glucosida strains, respectively, whe

50 and that adherence was inhibited by anti-M. haemolytica antibody.

51 inning, while their antibody responses to P. haemolytica antigens were delayed.

52 revealed that NETs formed in response to M. haemolytica are capable of trapping and killing a portio

53 i-Lpp1 antibodies significantly inhibited M. haemolytica binding to BBEC monolayers.

54 The leukotoxin (LktA) produced by Mannheimia haemolytica binds to bovine lymphocyte function-associat

55 kotoxin secreted by Mannheimia (Pasteurella) haemolytica binds to the intact signal peptide and cause

56 was surface exposed, was conserved among P. haemolytica biotype A serotypes, and had porin activity

57 PomB, respectively), were extracted from P. haemolytica by solubilization in N-octyl polyoxyl ethyle

58 d streptomycin (SmR) resistant plasmid of P. haemolytica called pYFC1.

59 lines of cattle genetically resistant to M. haemolytica-caused pneumonia, which inflicts an economic

60 demonstrate that the leukotoxin (LKT) of M. haemolytica causes NET formation by bovine neutrophils i

61 Mannheimia haemolytica causes pneumonia in domestic and wild rumina

62 hanced subsequent trapping and killing of M. haemolytica cells in bovine NETs.

63 e different bovine immune sera with whole P. haemolytica cells resulted in a reduction of bovine immu

64 onocytes, also formed METs in response to M. haemolytica cells.

65 o LKT trapped and killed a portion of the M. haemolytica cells.

66 duced NET formation in response to LKT or M. haemolytica cells.

67 istically with resistance to experimental P. haemolytica challenge in cattle.

68 A Pasteurella haemolytica cosmid clone that activates leukotoxin trans

69 Cattle vaccinated with live P. haemolytica developed a significant increase in serum an

70 Currently used vaccines against M. haemolytica do not provide complete protection against t

71 o previously described virulence factors, M. haemolytica encodes adhesins, including the filamentous

72 s recognize a protein in all serotypes of P. haemolytica except serotype 11.

73 consequence of the unusual codon usage in P. haemolytica genes.

74 The leukotoxin operon of M. haemolytica has a complex mosaic structure and has been

75 n of chromosomal gene fusions in Pasteurella haemolytica has been devised and used to create an lktC:

76 ) system of the bovine pathogen, Pasteurella haemolytica, have been identified immediately downstream

77 The P. haemolytica hsdMSR genes were mapped using transposon Tn

78 c lesions and mortality caused by Mannheimia haemolytica in bighorn sheep (BHS; Ovis canadensis) are

79 ole for the lipopolysaccharide (LPS) from P. haemolytica in the induction of proinflammatory cytokine

80 P. haemolytica incubated with H-DDDDDD-OH in zinc saline so

81 n plasma and feces in healthy and Mannheimia haemolytica infected calves.

82 Lesional lung tissue of M. haemolytica-infected BHS contained significantly higher

83 e for the severity of the lung lesions in M. haemolytica-infected BHS.

84 ression of PIC was induced at 2 h p.i. in P. haemolytica-infected cattle and continued to 4 h p.i.

85 in the bloodstream of dairy calves during M. haemolytica infection are reflected in the NIR spectral

86 indicate that the host-specific nature of M. haemolytica infection may result at least partially from

87 on of some PIC genes, as a consequence of P. haemolytica infection.

88 HS), since they are highly susceptible to M. haemolytica infection.

89 ral to PMN recruitment to the lung during M. haemolytica infection.

90 Genes identified in Mannheimia haemolytica infections (97) were involved in activating

91 and TNF-alpha genes were not increased in P. haemolytica-inoculated CD18(-) cattle lungs compared to

92 The induction of gene expression with P. haemolytica inoculation was more prominent in CD18(-) ca

93 emonstrated that serotype A1, but not A2, M. haemolytica invades differentiated BBECs by transcytosis

94 Mannheimia haemolytica is a key pathogen in the bovine respiratory

95 Mannheimia haemolytica is an important member of the bovine respira

96 Pasteurella haemolytica is an important respiratory pathogen of catt

97 The leukotoxin of Mannheimia haemolytica is an important virulence factor that contri

98 n (Lkt) secreted by Mannheimia (Pasteurella) haemolytica is an RTX toxin which is specific for rumina

99 The leukotoxin of Pasteurella (Mannheimia) haemolytica is believed to play a significant role in pa

100 Analysis of the genome indicates that M. haemolytica is naturally competent, as genes for natural

101 M. haemolytica is one of the causative agents of bovine res

102 M. haemolytica is surrounded by a polysaccharide capsule, a

103 Mannheimia haemolytica is the etiological agent of pneumonic pasteu

104 nant-specific leukotoxin (Lkt) of Mannheimia haemolytica is the key virulence factor contributing to

105 Mannheimia haemolytica is the primary bacterial species associated

106 The Gram-negative bacterium Mannheimia haemolytica is the primary bacterial species associated

107 Pasteurella haemolytica is the principal bacterial pathogen in the b

108 Mannheimia haemolytica is the principal bacterial pathogen of the b

109 iratory disease (BRD) linked with Mannheimia haemolytica is the principal cause of pneumonia in cattl

110 ns of OmpA proteins from bovine and ovine M. haemolytica isolates are very different but are highly c

111 of various pathogenic and non-pathogenic M. haemolytica isolates of bovine and ovine origin.

112 polysaccharide capsule, in a selection of M. haemolytica isolates of various serotypes and grown unde

113 ing specificities of these antibodies for M. haemolytica isolates representing different OmpA subclas

114 pecificity of OmpA among bovine and ovine M. haemolytica isolates, recombinant proteins representing

115 different ribotypes were observed for the P. haemolytica isolates, while only one ribotype was observ

116 cent reports have shown that the Pasteurella haemolytica leukotoxin (LKT) and other RTX toxins bind b

117 Pasteurella (Mannheimia) haemolytica leukotoxin (Lkt) causes cell type- and speci

118 S) preparations of the RTX toxin Pasteurella haemolytica leukotoxin (LKT) contained LKT and LPS as th

119 The effects of Pasteurella haemolytica leukotoxin (LKT) on the activity of phosphol

120 xposure of bovine neutrophils to Pasteurella haemolytica leukotoxin (LKT) stimulates the production o

121 inant leukocytes to Mannheimia (Pasteurella) haemolytica leukotoxin (Lkt).

122 wo other RTX toxin proteins, the Pasteurella haemolytica leukotoxin (LktA) and the enterohemorrhagic

123 To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological act

124 Incubation of bovine leukocytes with P. haemolytica leukotoxin caused marked cytoplasmic membran

125 at encodes a transcriptional activator of P. haemolytica leukotoxin expression.

126 there may be a specific binding site for P. haemolytica leukotoxin on bovine but not on porcine or h

127 should reveal whether the presentation of M. haemolytica leukotoxin peptides to T(h) cells by Ovca-DR

128 The related RTX toxin, the Pasteurella haemolytica leukotoxin structural protein (LktA), can be

129 lower titer of antibodies against Mannheimia haemolytica leukotoxin, in comparison to domestic sheep

130 n-toxin (RTX) toxin family related to the M. haemolytica leukotoxin.

131 ecise subunit of bovine LFA-1 utilized by M. haemolytica Lkt as the functional receptor.

132 Recent evidence suggests that M. haemolytica LKT binding to bovine leukocytes is mediated

133 Therefore, P. haemolytica LKT binds rapidly to susceptible and to repu

134 Recent studies indicate that P. haemolytica Lkt binds to bovine CD18, the common subunit

135 us studies by us and others indicate that M. haemolytica Lkt binds to CD18, the beta subunit of bovin

136 t necessary for the cytotoxic activity of M. haemolytica Lkt but that it enhances the potency of the

137 erferon (IFN-gamma) on the interaction of M. haemolytica LKT with bovine peripheral blood neutrophils

138 -integrins is the functional receptor for M. haemolytica Lkt.

139 the biological response of bovine PMNs to M. haemolytica LKT.

140 In this study, the P. haemolytica lktC mutant was shown to be less virulent th

141 Our findings support a role for P. haemolytica LPS and TNF-alpha in the induction of IL-8 f

142 d the kinetics of IL-8 mRNA expression in P. haemolytica LPS-stimulated bovine alveolar macrophages a

143 ratory syncytial virus (BRSV) and Mannheimia haemolytica (MH), to generate a well-defined metabolomic

144 ymal damage caused by factors released by P. haemolytica, neutrophils contribute to the pathologic ch

145 a protein band is recognized by both anti-M. haemolytica OmpA and anti-Lpp1 antibodies.

146 Until now, specific P. haemolytica OMPs which elicit antibodies that function i

147 less cat cassette and then delivered into P. haemolytica on a shuttle vector.

148 e monocyte-derived macrophages exposed to M. haemolytica or its LKT.

149 S and DS were stimulated with heat-killed M. haemolytica or LPS.

150 tis, bovine viral diarrhea virus, Mannheimia haemolytica or Mycoplasma bovis.

151 The presence of antibodies against P. haemolytica outer membrane proteins (OMPs) correlates st

152 Research with M. haemolytica outer membrane proteins (OMPs) has shown tha

153 represent an important mechanism by which P. haemolytica overwhelms host defenses, contributing to th

154 nd IV alleles were associated with bovine M. haemolytica, ovine M. haemolytica, and M. glucosida stra

155 ive, reproducible models for the study of M. haemolytica pathogenesis has hampered efforts to better

156 to determine the role of sRNA, if any, in M. haemolytica pathogenesis.

157 toxin expression may provide insight into M. haemolytica pathogenicity.

158 existing structural annotation of Mannheimia haemolytica PHL213 based on experimental evidence.

159 isit the structural annotation of Mannheimia haemolytica PHL213, a bovine respiratory disease pathoge

160 osome by Cre recombinase expressed from a P. haemolytica plasmid.

161 rophil infiltration into the lungs during P. haemolytica pneumonia are poorly characterized.

162 d from neonatal calves with acute Mannheimia haemolytica pneumonia showed that rapid up-regulation of

163 ramphenicol resistance gene (cat, CmR) in P. haemolytica (pNF2200).

164 than in Control calves (10.4%) on d2, and M. haemolytica prevalence on d7 as compared to control calv

165 Western blot analysis of M. haemolytica proteins that bind to BBEC showed a dominant

166 Expression of the P. haemolytica R-M genes in E. coli was inefficient and is

167 nfection of the bovine lung with Pasteurella haemolytica results in an acute respiratory disorder kno

168 ncern, including Bartonella spp., Mannheimia haemolytica, Rhodotorula spp., Piroplasmida spp., Toxopl

169 Of the six P. haemolytica ribotypes, two ribotypes predominated.

170 Plasmid pNF2176 carries the P. haemolytica ROB-1 beta-lactamase gene (blaP, ApR) and pN

171 Mannheimia haemolytica serotype 1 (S1) is the most common bacterial

172 toxin and endotoxin derived from Pasteurella haemolytica serotype 1 are the primary virulence factors

173 Pasteurella haemolytica serotype 1 is the bacterial agent responsibl

174 Pasteurella haemolytica serotype 1 is the bacterium most commonly as

175 Pasteurella haemolytica serotype A1 (bovine strain OK) was incubated

176 describes the genome sequences of Mannheimia haemolytica serotype A2 isolated from pneumonic lungs of

177 scribed from cattle in Europe, and Moraxella haemolytica sp. nov.

178 ic pro-LKT (produced by an lktC mutant of M. haemolytica) stimulated MET formation.

179 The P. haemolytica strain used was a mutant serotype A1 from wh

180 th divergent lineages of bovine and ovine M. haemolytica strains, respectively, indicating a history

181 phy-tandem mass spectrometry, matched two M. haemolytica surface proteins: heat-modifiable outer memb

182 lture supernatant from a mutant strain of P. haemolytica that does not produce any detectable leukoto

183 culture filtrates from a mutant strain of P. haemolytica that does not produce biologically active le

184 he explosive proliferation of serotype A1 M. haemolytica that occurs within the bovine respiratory tr

185 Pasteurella haemolytica, the causative agent of shipping fever pneum

186 hi and bronchioles of lungs infected with P. haemolytica, three Holstein calves homozygous for bovine

187 OmpA and Lpp1 contribute to adherence of M. haemolytica to bovine respiratory epithelial cells.

188 Cattle were infected with P. haemolytica via fiberoptic deposition of organisms into

189 e lungs of CD18(+) cattle inoculated with P. haemolytica was greater than that in lungs of the CD18(-

190 ural gene (lktA) of Mannheimia (Pasteurella) haemolytica was investigated by nucleotide sequence comp

191 the bovine respiratory pathogen Pasteurella haemolytica, was cloned, and its nucleotide sequence was

192 ro-LKT produced by an DeltalktC mutant of M. haemolytica, we show that binding of unacylated pro-LKT

193 ved a significant reduction in killing of P. haemolytica when bovine immune serum that was depleted o

194 y surfactant is bactericidal for Pasteurella haemolytica when surfactant and bacteria mixtures are in

195 nds of the operon are highly conserved in M. haemolytica, which suggests that multiple horizontal exc

196 little is known about the interactions of M. haemolytica with airway epithelial cells of the respirat